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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Termofilia e termogênese pós-prandiais em Bothrops moojeni (Serpentes:Viperidae) em cativeiro / Thermogenesis and post-prandial termophly in Bothrops moojeni (Serpentes: Viperidae) in captivity

Daniel Rodrigues Stuginski 12 May 2009 (has links)
O estudo das temperaturas preferenciais e comportamento termofílico é essencial na compreensão da termobiologia das serpentes, todavia, a maioria dos trabalhos tem focado somente os animais diurnos e de clima temperado e pouco se sabe a respeito das serpentes noturnas tropicais e sub-tropicais. O presente estudo utilizou uma arena com gradiente térmico monitorada por vídeo para mensurar o intervalo térmico preferencial procurado durante os períodos de atividade e inatividade (Tset) e a resposta termofílica pós-prandial frente a diferentes quantidades de ingesta em 29 serpentes Bothrops moojeni . Os resultados obtidos mostraram que o intervalo térmico preferencial de B.moojeni diferiu conforme o fotoperíodo (20,93°C a 22,20°C durante o dia e 22,81°C a 24,42°C durante a noite) e foi similar a outros intervalos encontrados para outras serpentes crotalinae. Os dados mostraram que há uma correlação inversa entre intensidade da resposta termofílica pós-prandial e quantidade de alimento ingerido, uma vez que os animais alimentados com uma porção correspondente a 10% de seu peso corporal apresentaram maior resposta termofílica pós-prandial quando comparados àqueles que foram alimentados com porções maiores (20 e 40% do peso corporal). Esta diferença parece estar relacionada com a diminuição de mobilidade e a maior SDA (Specific Dynamic Action) nos animais que ingeriram a maior quantidade de alimento. As serpentes crotalinae são tidas como animais preponderantemente noturnos sendo que a maioria dos dados a respeito dessas atividades foram obtidos em pesquisas de campo. A detecção do período de atividade, assim como o conhecimento de como o estado nutricional influencia a taxa de trânsito, são extremamente importantes na compreensão da ecofisiologia destes animais. No estudo foram utilizadas 29 exemplares de Bothrops moojeni, monitoradas quanto ao horário e o tempo de deslocamento durante o jejum e nos 5 dias subseqüentes a alimentação. A alimentação das serpentes variou entre 10, 20 e 40% do peso da própria serpente. Os resultados confirmaram Bothrops moojeni como espécie essencialmente noturna, sendo que a atividade foi aumentada em mais de 45 vezes durante o período. O pico de atividade ocorreu entre as 19:00 e 20:00 horas, não havendo diferença estatística entre machos e fêmeas. Com relação à atividade pós-prandial foi notada diferença estatística nas taxas de deslocamento dos animais que comeram as maiores proporções (20-40%). Os resultados obtidos corroboram com os dados prévios obtidos em estudos de campo que pontuam Bothrops moojeni como um animal noturno e de baixo grau de mobilidade. A interação entre a quantidade de alimento ingerido pela serpente, o balanço energético e o grau de mobilidade dos animais é bastante complexa e discutível. As serpentes foram consideradas por muito tempo como incapazes de gerar calor através de seu metabolismo aeróbico, à exceção das pythons no choco. Entretanto, estudos recentes mostraram que algumas espécies são capazes de aumentar a temperatura corporal sem recorrer a fontes externas de calor ou contrações musculares. Esta termogênese ocorre devido aos enormes níveis metabólicos atingidos durante a digestão (Specific Dynamic Action - SDA). Em geral as serpentes têm um baixo custo energético de manutenção e a maioria das espécies é capaz de permanecer longo período em jejum e depois se alimentar de uma grande ingesta. A energia produzida durante a digestão destas grandes refeições pode gerar um significativo aumento na temperatura corpórea dos animais. O presente estudo investigou a termogênese pós-prandial em Bothrops moojeni, através de imagens térmicas tomadas em um ambiente termoestável a 30°C. As 12 serpentes foram divididas em 2 grupos e acompanhadas pelas 72 horas após ingerirem refeições equivalentes a 10-60 20% e 30-40% de seu próprio peso, respectivamente. Os resultados mostraram que a resposta termogênica pós-prandial levou a um aumento de 1,6°C da temperatura da pele das serpentes. O pico térmico ocorreu entre 33 e 36 horas após a alimentação em ambos os grupos e a duração da termogênese variou conforme o tamanho da ingesta. Os resultados mostraram uma alta correlação entre a termogênese pós-prandial e a SDA das serpentes. O aumento significativo da temperatura corporal e a sua manutenção por certo tempo sugerem que estes animais são capazes de manter altas taxas metabólica durante a digestão independente da temperatura do ambiente. / The study of the preferred temperatures and thermophilic behavior is essential to understand the snakes thermal biology. Although some studies have been reported, most of them are focused in temperate and diurnal species. Thus, little is known about the tropical and sub-tropical nocturnal species. In the present study , a video-monitored arena with a thermal gradient was used to investigate the preferred thermal range during activity and inactivity (Tset) and the post-prandial thermophilic response after different levels of food intake in 29 Brazilian lanceheads (Bothrops moojeni). The results showed that the preferred thermal range of B.moojeni changes depending on the photoperiod (20.93°C to 22.20°C in daytime and 22.81°C to 24.42°C in night time), being similar to other crotalinae snakes. Our data suggests that there is an inverse correlation between the post-prandial thermophilic response intensity and food intake, as animals fed with a portion corresponding to 10% of its bodyweight presented a higher thermophilic response in comparison to those who were fed with larger portions (20 and 40% of bodyweight). This difference may be related to the decreased mobility and increased SDA (Specific Dynamic Action) in animals that consumed the largest amount of food. Crotalinae are considered to be mainly nocturnal and most of the gathered data on these snakes came from field work. The knowledge of activity pattern and how nutritional status affect wandering rate are determinant to understand the ecophysiology of the snakes. In this study the daily activity pattern was followed in 29 subjects of the lancehead Bothrops moojeni in order to record displacement rate in fasting and feed snakes for five days after feeding meal of 10, 20 and 40% of their own body weight. The results show that B.moojeni is prevalently nocturnal, and activity increased 45 times at night, peaking between 19 to 20 h, in both males and females. Activity level changed significantly after feeding in snakes which ate a larger meal (20-40%). The results confirm previous field data for B.moojeni as a nocturnal species with low mobility. The complex interactions between the amount of food taken by the snake, energetic balance and mobility are discussed. Snakes were for long considered incapable to generate heat fueled by aerobic metabolism, except for brooding pythons. However, recent studies proved that some species are able to elevate their body temperature without muscle contraction or external heat source. Such thermogenic capacity is supported by high metabolic rate that follows digestion and assimilation of food (Specific Dynamic Action - SDA). Snakes have a low maintenance cost and most species can stand for long fasting time, particularly after ingesting a great meal. The energy produced during digestion of such meal can generate significant increase in body temperature. The present study investigated thermogenic response after feeding in the Brazilian lancehead, Bothrops moojeni, using thermal images taken in temperature controlled environment at 30°C. The 12 snakes were divided into two groups and followed for 72 hours after fed a meal representing 10-20% and 30-40% of their body weight, respectively. The results showed thermogenic digestive response with an increase up to 1.6 °C of skin temperature. Thermal heat production peak occurred between 33 to 36 hours after feeding in both groups, and the duration of thermogeny varied with the meal size. The result showed high correlation between thermogenic data and SDA in snakes. The significant increase of body temperature after feeding and its maintenance for extended time suggest a physiological advantage to keep high metabolic rate despite of environmental temperature.
22

The art of maintaining a successful marriage in the Seventh-Day Adventist Church

Tembo, Lysant Molly Langwell 08 1900 (has links)
Making a successful Christian marriage is a major challenge that faces the Seventh Day Adventist church (SDA Church) of Malawi. The colonial government of Nyasaland (Malawi) created weak marriages, promoting high divorce rates by its own practices during its era, which have remained to this day. The failure of secular marriages endangers the success of SDA Christian marriages. Little has been done by the Church to educate its members concerning successful Christian marriage. This study focuses on educating the church to deal with the problems that cause marriage failure in the SDA Church. The Malawi government is another tool that the church could use to address marriage failure. I have used the Bible, and scientific research methods to suggest workable solutions for Christian marriage. / Practical Theology / M.Th. (Practical Theology)
23

Contribution à l'introduction de concepts mécaniques dans les Micro et Nano Systèmes

Buchaillot, Lionel 29 March 2004 (has links) (PDF)
Le groupe Microsystèmes Silicium de l'Institut d'Électronique, de Microélectronique du Nord (IEMN - maintenant N=Nanotechnologie) a axé sa recherche sur les micro-actionneurs et leur environnement. Cela nous permet d'étudier des problématiques amont liées au micro-actionnement, à son interaction technologique, ainsi que des voies applicatives telles que les microsystèmes pour les télécommunications, la microrobotique, entre autres... <br />Dans la suite de cette introduction, quelques précisions sont données sur la terminologie de notre domaine de recherche, puis un bref historique retrace le développement de la recherche sur les microsystèmes dans le monde et en France.<br />Outre cette introduction, ce rapport se compose principalement de trois chapitres. Le premier, consacré aux micro-actionneurs, présente une partie des travaux effectués sur des études conceptuelles exploitant le potentiel de différents modes d'actionnement : transition de phase; électrostatique et thermique. <br />Le second chapitre décrit les activités de recherche effectuées dans le thème applicatif des microsystèmes pour les télécommunications. Il s'agit de travaux soutenus par de nombreux contrats de recherche nationaux ou européens. Deux composants en particulier ont été étudiés par trois doctorants. Emmanuel Quévy a étudié des résonateurs électromécaniques destinés à une application de filtrage dans la gamme intermédiaire de fréquence de la téléphonie mobile. Vincent Agache a étudié des résonateurs dont les fréquences propres se situent au voisinage du GHz. Les premières recherches effectuées à l'IEMN sur des microcommutateurs pour les signaux hyperfréquences sont également présentées<br />Le troisième chapitre est dédié aux résultats obtenus par Olivier Millet sur l'évolution de la raideur de microstructures soumises à un chargement cyclique. Il s'agit d'une partie de l'étude transversale des mécanismes de défaillance des microsystèmes destinée à la modélisation du phénomène de fatigue dans les microstructures.<br />Enfin, quelques perspectives de recherche impliquant les micro-actionneurs dans les microsystèmes seront présentées, suivies d'une conclusion
24

Studies on Baltic Sea mysids

Ogonowski, Martin January 2012 (has links)
Mysid shrimps (Mysidacea, Crustacea) are efficient zooplanktivores in both marine and freshwater systems as well as lipid rich prey for many species of fish.  Although some efforts have been made to study the role of mysids in the Baltic Sea, very few studies have been carried out in recent time and there are still knowledge gaps regarding various aspects of mysid ecology. This thesis aims to explore some of these gaps by covering a mixture of topics. Using multifrequency hydroacoustics we explored the possibility to separate mysids from fish echoes and successfully established a promising and effective method for obtaining mysid abundance/biomass estimates (paper I). An investigation of the current mysid community in a coastal area of the northern Baltic proper (paper II) demonstrated that the formerly dominant, pelagic mysid Mysis mixta had decreased substantially (~50%) in favor for phytoplanktivorous, juvenile Neomysis integer and Mysis relicta sp. By examining different aspects of mysid behavior, we studied the vertical size distribution of mysids in the field and found that size increased with depth/declining light, irrespective of temperature; indicating that their vertical size distribution primarily is a response to predation (paper II). In paper III, a combination of ecological and genetic markers was used to investigate intraspecific differences in migratory tendency. Both marker types indicated that some part of the Mysis salemaai population is sedentary on the bottom and that this strategy is a phenotypically plastic but persistent trait, analogous to the partial migrations seen in many birds and fishes. In paper IV a temperature and weight specific respiration model was developed for the littoral Praunus flexuosus. Routine respiration was moreover elevated by post-prandial effects (specific dynamic action) for longer times than previously suggested. Consequently, ignoring such effects could significantly bias respiration measurements. / At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper  2: Accepted; Paper 3: Submitted; Paper  4: Accepted
25

Making it happen prevention of mother to child transmission of HIV in rural Malawi /

Kasenga, Fyson, January 2009 (has links)
Diss. (sammanfattning) Umeå : Umeå universitet, 2009. / Felaktigt serienummer 1251. Härtill 4 uppsatser.
26

The pedagogical use of ICTs for teaching and learning within grade eight mathematics in South African schools / V. Cassim

Cassim, Verona January 2010 (has links)
Information and communication technology (ICT) has become part of education as it has, in many cases, become the mode of choice of communication with people in all spheres of life. It provides teachers with the opportunity to access information from a vast array of resources that assists them in their teaching practices. Education in South Africa is constantly transforming to new requirements from the National Department of Education (NDoE). The fundamentals of Outcomes Based Education are lifelong learning and the development of 21st century skills that allow learners to use information for different contexts. ICT enables teachers and learners to access computer systems to develop skills, interact with their peers, colleagues, and the global society. Even though teachers know the value of ICT in teaching and learning, the pedagogical use of ICT in South African schools remains limited. In the SITES 2006, South African teachers acknowledged that they were enthusiastic to explore new ways to make teaching and learning more interesting, but that they encountered many barriers that hinder the pedagogical use of ICT for mathematics. This research has determined that the teachers’ ICT pedagogical knowledge contributed towards more effective teaching and learning practices of mathematics in South African schools. The study also describes how insufficient ICT pedagogical knowledge affected teachers’ confidence to explore ICT tools. This study followed a secondary data analysis (SDA) of the Second International Information Technology in Education Study of 2006 (SITES 2006) data from the 640 participating mathematics teachers in South Africa. The correlated data describes the technological pedagogical content knowledge (TPCK) of mathematics teachers while making use of ICT. Continuous professional teacher development is required to focus on the attainment of information technology pedagogical knowledge to further the use of ICT on the teaching of Mathematics. The study also indicates that South Africa lags far behind the other 22 countries that participated in SITES 2006. / Thesis (M.Ed.)--North-West University, Potchefstroom Campus, 2011.
27

The pedagogical use of ICTs for teaching and learning within grade eight mathematics in South African schools / V. Cassim

Cassim, Verona January 2010 (has links)
Information and communication technology (ICT) has become part of education as it has, in many cases, become the mode of choice of communication with people in all spheres of life. It provides teachers with the opportunity to access information from a vast array of resources that assists them in their teaching practices. Education in South Africa is constantly transforming to new requirements from the National Department of Education (NDoE). The fundamentals of Outcomes Based Education are lifelong learning and the development of 21st century skills that allow learners to use information for different contexts. ICT enables teachers and learners to access computer systems to develop skills, interact with their peers, colleagues, and the global society. Even though teachers know the value of ICT in teaching and learning, the pedagogical use of ICT in South African schools remains limited. In the SITES 2006, South African teachers acknowledged that they were enthusiastic to explore new ways to make teaching and learning more interesting, but that they encountered many barriers that hinder the pedagogical use of ICT for mathematics. This research has determined that the teachers’ ICT pedagogical knowledge contributed towards more effective teaching and learning practices of mathematics in South African schools. The study also describes how insufficient ICT pedagogical knowledge affected teachers’ confidence to explore ICT tools. This study followed a secondary data analysis (SDA) of the Second International Information Technology in Education Study of 2006 (SITES 2006) data from the 640 participating mathematics teachers in South Africa. The correlated data describes the technological pedagogical content knowledge (TPCK) of mathematics teachers while making use of ICT. Continuous professional teacher development is required to focus on the attainment of information technology pedagogical knowledge to further the use of ICT on the teaching of Mathematics. The study also indicates that South Africa lags far behind the other 22 countries that participated in SITES 2006. / Thesis (M.Ed.)--North-West University, Potchefstroom Campus, 2011.
28

Energética alimentar em Rã-manteiga, Leptodactylus latrans (Anura, Leptodactylidae) / Food energy of leptodatylid frog, Leptodactylus latrans (Anura, Leptodactylidae)

Timpone, Luá Taibo [UNESP] 31 October 2016 (has links)
Submitted by Luá Taibo Timpone null (101010913@rc.unesp.br) on 2017-01-18T20:52:13Z No. of bitstreams: 1 Dissertação_VersãoFinal_Completa_Luá.pdf: 1544050 bytes, checksum: 3ff859d190848f5e447a8680e9b509cb (MD5) / Rejected by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br), reason: Solicitamos que realize uma nova submissão seguindo as orientações abaixo: No campo “Versão a ser disponibilizada online imediatamente” foi informado que seria disponibilizado o texto completo porém no campo “Data para a disponibilização do texto completo” foi informado que o texto completo deverá ser disponibilizado apenas 12 meses após a defesa. Caso opte pela disponibilização do texto completo apenas 12 meses após a defesa selecione no campo “Versão a ser disponibilizada online imediatamente” a opção “Texto parcial”. Esta opção é utilizada caso você tenha planos de publicar seu trabalho em periódicos científicos ou em formato de livro, por exemplo e fará com que apenas as páginas pré-textuais, introdução, considerações e referências sejam disponibilizadas. Se optar por disponibilizar o texto completo de seu trabalho imediatamente selecione no campo “Data para a disponibilização do texto completo” a opção “Não se aplica (texto completo)”. Isso fará com que seu trabalho seja disponibilizado na íntegra no Repositório Institucional UNESP. Por favor, corrija esta informação realizando uma nova submissão. Agradecemos a compreensão. on 2017-01-24T13:40:19Z (GMT) / Submitted by Luá Taibo Timpone null (101010913@rc.unesp.br) on 2018-03-02T20:05:16Z No. of bitstreams: 1 Dissertação_VersãoFinal_Completa_Luá.pdf: 1544050 bytes, checksum: 3ff859d190848f5e447a8680e9b509cb (MD5) / Approved for entry into archive by Vivian Rosa Storti null (vstorti@reitoria.unesp.br) on 2018-04-04T12:42:54Z (GMT) No. of bitstreams: 1 timpone_lt_me_rcla.pdf: 1544050 bytes, checksum: 3ff859d190848f5e447a8680e9b509cb (MD5) / Made available in DSpace on 2018-04-04T12:42:54Z (GMT). No. of bitstreams: 1 timpone_lt_me_rcla.pdf: 1544050 bytes, checksum: 3ff859d190848f5e447a8680e9b509cb (MD5) Previous issue date: 2016-10-31 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O tamanho do alimento e a temperatura corpórea são dois fatores que reconhecidamente influenciam a resposta metabólica pós-prandial dos vertebrados ectotérmicos. Leptodactylus latrans é uma espécie generalista que consome desde invertebrados até pequenos vertebrados, de forma que sua dieta é composta por itens de tamanho variável, os quais sempre são ingeridos inteiros. A temperatura corpórea de L. latrans está sujeita a disponibilidade de nichos térmicos adequados nos ambientes onde ocorre, os quais são possivelmente explorados através da termorregulação comportamental. No presente estudo, nós avaliamos os efeitos do tamanho da presa e da temperatura corpórea sobre a resposta metabólica pós-prandial, em indivíduos adultos da rã-manteiga, Leptodactylus latrans (Anura, Leptodactylidae) através da quantificação das taxas de consumo de oxigênio antes e após a alimentação. A resposta metabólica pós-prandial de L. latrans pode ser caracterizada por um rápido aumento nas taxas de consumo de oxigênio logo após a ingestão do alimento, até atingir valores máximos entre 85,93 ± 9,92 e 224,54 ± 26,08 mLO2.kg-1. h-1, dentro de 1-2 dias. Após este pico, houve um retorno paulatino da taxa metabólica aos níveis de jejum entre 3 a 7 dias após a alimentação. De maneira geral, encontramos que a ingestão de presas maiores causa um maior incremento metabólico e aumenta a duração da digestão. Já o incremento da temperatura é acompanhado pela diminuição da duração da digestão às expensas de taxas metabólicas pós-prandiais mais elevadas. Tanto em um caso como no outro, não encontramos indicações de que fatores cardiorrespiratórios pudessem atuar na limitação do tamanho da presa ou na temperatura na qual a digestão pode ocorrer. No entanto, é plausível que outros fatores de cunho ecológico sejam relevantes na consideração dos efeitos do tamanho do alimento e da temperatura na digestão de L. latrans. Em especial, um maior retorno energético absoluto ao se alimentar de presas maiores e a diminuição do tempo de digestão em temperaturas mais elevadas, ambos aspectos documentados em L. latrans, podem representar benefícios ecológicos importantes. / Meal size and body temperature affect markedly the post-prandial metabolic response of ectothermics vertebrates. Leptodactylus latrans is a generalist anuran species feeding on diverse prey itens, from invertebrates to small vertebrates, with considerable size variation and that are always ingested whole. The body temperature of L. latrans is largely dependent of the availability of thermal niches in the environment, which are possibly explored by means of behavioral thermoregulation. In the present study, we investigated the effects of meal size and body temperature on the post-prandial metabolic response of adults of the Neotropical leptodatylid frog, Leptodactylus latrans (Anura, Leptodactylidae) by quantifying the rates of oxygen consumption before and after meal ingestion. The post-prandial metabolic response of L. latrans can be characterized by the rapid increase in the rates of oxygen consumption shortly after meal ingestion, until reach peak between 85,93 ± 9,92 e 224,54 ± 26,08 mLO2.kg-1. h-1, within 1-2 days post-feeding. Thereafter, metabolism gradually return to the pre-feeding values within 3 to 7 days post-feeding. In general, we found that the ingestion of larger meals were followed by greater metabolic increment and by longer digestion duration. Temperature increment caused digestion duration to decrease at the expenses of greater post-prandial metabolic rates. In both cases, we did not found evidences that cardiorespiratory compromises could limit meal size or the temperature in which meal digestion could occur. On the other hand, some ecological considerations seems to be relevant in considering the effects of meal size and body temperature on the digestion of L. latrans. More specifically, the greater gross energetic gain when feeding on larger prey and the shortening of digestion duration at higher temperatures, both observed in L. latrans, are likely to yield substantial ecological advantages. / Capes Demanda Social
29

The art of maintaining a successful marriage in the Seventh-Day Adventist Church

Tembo, Lysant Molly Langwell 08 1900 (has links)
Making a successful Christian marriage is a major challenge that faces the Seventh Day Adventist church (SDA Church) of Malawi. The colonial government of Nyasaland (Malawi) created weak marriages, promoting high divorce rates by its own practices during its era, which have remained to this day. The failure of secular marriages endangers the success of SDA Christian marriages. Little has been done by the Church to educate its members concerning successful Christian marriage. This study focuses on educating the church to deal with the problems that cause marriage failure in the SDA Church. The Malawi government is another tool that the church could use to address marriage failure. I have used the Bible, and scientific research methods to suggest workable solutions for Christian marriage. / Philosophy, Practical and Systematic Theology / M.Th. (Practical Theology)
30

Efeitos da temperatura e do estado metabólico sobre a função cardíaca do jacaré-de-papo-amarelo (Caiman latirostris)

Lopes, André Guelli 28 January 2014 (has links)
Made available in DSpace on 2016-06-02T19:22:10Z (GMT). No. of bitstreams: 1 5716.pdf: 1902370 bytes, checksum: 9a5b25a55be97bb37553eeb1f8130b09 (MD5) Previous issue date: 2014-01-28 / Financiadora de Estudos e Projetos / Phenomena as feeding and thermoregulation can challenge both the cardiac output and heart rate (fH). Postprandial changes in many physiological patterns are typically recorded for ectotherms, although predominantly studied in snakes. Furthermore, thermoregulation is a critical process to ensure different survival and reproduction strategies from reptiles. Ventricular preparations in vitro were used to investigate the inotropic plasticity at 30 ºC and 25 ºC and during fasting (30 days fasting group) and after feeding (2 days digesting group). No significant differences were verified in the relative ventricular mass (RVM) after feeding. The peak tension (Fc) was significantly higher and rates (dFc/dt) of contraction (TC) and relaxation (TR) were faster at 25° C than at 30° C for both experimental groups. However, feeding caused significant increase in peak tension (PT) at both temperatures (396.7 % at 30 ºC and 416.7 % at 25 ºC). A significant post-rest potentiation was recorded for digesting group at 25 ºC (121.0 ± 6.4 %), which was inhibited by ryanodine (86.0 ± 2.9 %). At 30 ºC, myocytes from fasting group were able to support higher frequencies (up to 1.6 Hz) than at 25 ºC (up to 1.0 Hz), even with the sarcoplasmic reticulum (SR) blocked by 10 &#956;M ryanodine (RYA). Data from digestion group showed greater values of the Fc and reached higher frequencies than fasting group, at both temperatures. However, pre-treatment with RYA decreased PT in frequencies between 1.4 and 1.8 Hz for digesting group at 30 ºC. Sarcolemma mechanisms were more representative in the fasting group at 25 ºC, but the SR must contribute to increased speed of contractility under those conditions. During SDA, the SR seem representative at 25 ºC, however the positive inotropic effect caused by SDA enlarged sarcolemma mechanisms representativeness. The SR significance at 30 °C seems critical during high frequencies. At this temperature, the SDA increases the contractility efficiency, even with the RS blocked, despite the relaxation and Fc development at high frequencies been present impaired. Our results indicate that Caiman latirostris myocytes show large inotropic plasticity at different temperatures, frequencies and metabolic states. / A digestão e a termorregulação são processos que podem desafiar tanto o débito quanto a frequência cardíaca (fH). Alterações pós-prandiais em diferentes padrões fisiológicos são comumente identificadas para ectotérmicos, embora sejam predominantemente estudados em serpentes. Além disso, a termorregulação é um processo crítico para garantir diferentes estratégias de sobrevivência e reprodução nos répteis. Preparações ventriculares in vitro foram utilizadas para investigar a plasticidade inotrópica dos miócitos ventriculares do Caiman latirostris a 25 e 30 °C, durante o período pós- absortivo (30 dias - após a alimentação) e após a alimentação (2 dias - grupo digestão). A massa ventricular relativa (MVR) após 2 dias da alimentação não apresentou diferenças significativas em relação ao grupo pós-absortivo. A força de contração (Fc) foi significativamente maior e as taxas (dFc/dt) de contração (TC) e relaxamento (TR) foram mais rápidas a 25 °C do que a 30 °C, para ambos os grupos experimentais. No entanto, a alimentação causou aumento significativo na força de contração (Fc) em ambas as temperaturas (316.7% a 30 °C e a 296.7% 25 °C). Uma significativa potenciação pós-pausa foi identificada no grupo digestão a 25 °C (121.0 ± 6.4 %), que foi inibida pela rianodina (86.0 ± 2.9 %). No grupo pós-absortivo a 30 °C, os miócitos foram capazes de suportar frequências de estimulação superiores (até 1.6 Hz) às suportadas a 25 °C (até 1.0 Hz), mesmo com a função do retículo sarcoplasmático (RS) bloqueada pela adição de 10 &#956;M de rianodina (RYA). As preparações ventriculares do grupo digestão apresentaram valores maiores de Fc e alcançaram frequências superiores do que o grupo pós-absortivo, em ambas as temperaturas. Contudo, o pré-tratamento com RYA reduziu a Fc em frequências entre 1,4 e 1,8Hz no grupo digestão a 30 °C. Os mecanismos sarcolemais são mais representativos no grupo pós-absortivo a 25 °C, porém o RS pode contribuir com o aumento da velocidade da contratilidade nessas condições. Durante a SDA, o RS parece representativo nessa condição de temperatura, porém a SDA causou efeito inotrópico positivo e ampliou a representatividade dos mecanismos sarcolemais. A importância do RS a 30 °C parece crítica apenas em altas frequências de estimulação. Nessa temperatura, a SDA garante a eficiência da contratilidade mesmo com o RS bloqueado, apesar do prejuízo significativo no desenvolvimento da Fc e do relaxamento em altas frequências. Nossos resultados indicam que os miócitos ventriculares do Caiman latirostris apresentam ampla plasticidade inotrópica, capaz de assegurar o débito cardíaco em diferentes temperaturas, frequências e estados metabólicos.

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