Danos mitocondriais e diminuição nos níveis de ATP têm sido recentemente atribuídos à sertralina. Nós investigamos o efeito da sertralina em plaquetas humanas, já que estas células demonstram várias similaridades funcionais com neurônios e astrócitos. Os efeitos da sertralina em diferentes parâmetros foram investigados em plaquetas lavadas de 18 voluntários saudáveis do sexo masculino, depois de 24h de exposição ao fármaco. A toxicidade da sertralina foi observada apenas nas concentrações mais altas, 30 e 100μM, nas quais a viabilidade das plaquetas foi significantemente reduzida a 76 ± 3% e 20 ± 2 %, respectivamente. As mesmas concentrações reduziram o ATP a 73 ± 3% e 13 ± 2 %, respectivamente. Os valores basais de glicogênio não foram afetados significativamente pelo tratamento com sertralina. A captação de glutamato foi reduzida de forma significativa após o tratamento com 3, 30 e 100 μM, em 28 ± 6 %, 32 ± 5% e 54 ± 4 %, respectivamente. Nosso estudo demonstra que a sertralina em concentrações terapêuticas não compromete a viabilidade plaquetária, porém seu uso não pode ser considerado isento de risco, pois em um cenário no qual os níveis de glutamato extracelular estão potencialmente aumentados, a sertralina poderia agravar uma condição excitotóxica / Mitochondrial damage and fall in ATP levels have been recently attributed to sertraline. We investigated the effect of sertraline on human platelets, since these cells display several functional similarities with neurons and astrocytes. The effects of sertraline on different parameters were investigated in washed platelets from 18 healthy male volunteers, after 24 h of drug exposure. Sertraline toxicity was observed only at the highest concentrations, 30 and 100 μM, which significantly reduced platelet viability to 76 ± 3% and 20 ± 2%, respectively. The same concentrations significantly decreased total ATP to 73 ± 3% and 13 ± 2%, respectively. Basal values of glycogen were not significantly affected by sertraline treatment. The glutamate uptake was significantly reduced after treatment with 3, 30 and 100 μM, by 28 ± 6%, 32 ± 5% and 54 ± 4%, respectively. Our study showed that sertraline at therapeutic concentrations does not compromise platelet viability, but its use may not be without risk, since in a scenario where extracellular glutamate levels are potentially increased, sertraline might aggravate an excitotoxic condition.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-22T12:42:37Z No. of bitstreams: 1 2016 - Ericson Ramos de Mello.pdf: 4027891 bytes, checksum: 7ce6fcb724a96319669aef0ec318a39e (MD5) / Made available in DSpace on 2017-09-22T12:42:37Z (GMT). No. of bitstreams: 1 2016 - Ericson Ramos de Mello.pdf: 4027891 bytes, checksum: 7ce6fcb724a96319669aef0ec318a39e (MD5) Previous issue date: 2016-09-29 / The Centers for Wild Animals Screening (CETAS) on jurisdiction of the Brazilian Institute of Environment and Renewable Resources (IBAMA), are responsible for the receipt and disposal of wild animals that are victims of trafficking throughout the national territory. The CETAS of Serop?dica (CETAS-RJ) is the only place responsible for the receipt and disposal of wild animals that are victims of trafficking in the state of Rio de Janeiro. This study aimed to characterize the species of wildlife, seized or voluntarily surrendered, 2008-2014 in CETAS of Serop?dica ? Rio de Janeiro. The total effective entries in the Wild Animal Screening Center was 39.777 (thirty-nine thousand, seven hundred seventy-seven) animals during the years 2008 to 2014, with the annual average of the last 7 years, 5.682 entries. Among the animals received, 91,25% (36.295) were birds, 5,47% (2.177) reptiles, 3,14% (1.248) mammals, 0,13% (53) invertebrates; between crustaceans, insects and arachnids and 0,01% (4) indeterminate information from seizures, deliveries or redemptions. The seizure was the origin most represented during the study period, with 88,75% (35.302) animals and an average of 5.043 animal per year. The Environmental Police Command (CPAM), along with other battalions of military and Civilian Police were the most representative bodies in the fight against wildlife trafficking. Receipt of birds between the years, identified 24 orders, 54 families and 255species of birds. The order Passeriformes was the one that had the highest number of incoming animals (92,22%), having predominance of Thaupidae family with 85,54% (28.634), followed by family Icteridae 3,41% (1.143). The collared (Sporophila caerulescens), the Saffron Finch (Sicalis flaveola), the Crack-iron-true (Saltator similis), the Songbird (Sporophila angolensis), accounted for about 78,18% of passerines, 61,15% of the seized birds and 56,60% of total birds received in CETAS ? RJ, during the study period / Os Centros de Triagem de Animais Silvestres (CETAS), sob jurisdi??o do Instituto Brasileiro de Meio Ambiente e Recursos Renov?veis (IBAMA), s?o respons?veis pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico em todo territ?rio Nacional. O CETAS de Serop?dica (CETAS-RJ), localizado na Floresta M?rio Xavier ? o ?nico local respons?vel pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico no Estado do Rio de Janeiro. Objetivou-se caracterizar as esp?cies, da fauna silvestre, apreendidas ou entregues voluntariamente, de 2008 a 2014 no CETAS de Serop?dica ? Rio de Janeiro. O total de entradas efetivas no Centro de Triagem de Animais Silvestres foi de 39.777 (Trinta e nove mil, setecentos e setenta e sete) animais, durante os anos de 2008 a 2014, tendo como m?dia anual, dos ?ltimos 7 anos, 5.682 entradas. Dentre os animais recebidos, 91,25% (36.295) foram aves, 5,47% (2.177) r?pteis, 3,14% (1.248) mam?feros, 0,13% (53) invertebrados; entre crust?ceos, insetos e aracn?deos provenientes de apreens?es, entregas ou resgates. A apreens?o foi a proced?ncia com maior representa??o durante o per?odo estudado, com 88,75% (35.302) animais e uma m?dia de 5.043 animais por ano. O Comando de Pol?cia Ambiental (CPAM), juntamente com outros batalh?es da pol?cia militar e civil, foram os ?rg?os mais representativos no combate ao tr?fico de animais silvestres. O recebimento de aves entre 2008 e 2014 representou cerca de 91,25% (36.295) das entradas em rela??o ao total de todas as classes entre os anos, sendo identificadas 24 ordens, 54 fam?lias e 255 esp?cies de aves. A ordem Passeriformes foi a que teve o maior n?mero de animais recebidos (92,22%), havendo predomin?ncia das fam?lias, Thaupidae com 85,54% (28.634) e Icteridae 3,41% (1.143). O coleirinho (Sporophila caerulescens), o can?rio-da-terra-verdadeiro (Sicalis flaveola), o trinca-ferro-verdadeiro (Saltator similis), o curi? (Sporophila angolensis), o tiziu (Volatinia jacarina), o tico-tico (Zonotrichia capensis), corresponderam a cerca de 78,18% do total de Passeriformes, 61,15% apreendidos e 56,60% das aves recebidas no CETAS - RJ, no per?odo do estudo.

Severe Traumatic Brain Injury (sTBI) is a major cause of mortality and morbidity. At the Department of Neurosurgery Umeå University Hospital subjects with sTBI are treated with an intracranial pressure (ICP) guided therapy based on physiological principles, aiming to optimise the microcirculation of the brain so avoiding secondary brain injuries. The investigations in this thesis are unique in the sense that all patients with sTBI were treated according to the guidelines of an ICP targeted therapy based on the “Lund concept”. As the treatment is based on normalisation of the ICP, the accuracy and reliability of the measuring device is of outmost importance. Therefore the accuracy, drift, and complications related to the measuring device was prospectively studied (n=128). The drift was 0,9 ± 0,2 mmHg during a mean of 7,2 ± 0,4 days and the accuracy high. No clinical significant complications were noted. In 1997 uni- or bilateral decompressive hemi-craniectomy (DC) was introduced into the treatment guidelines. The effect of DC on the ICP and outcome was retrospectively analysed for subjects with sTBI treated 1998-2001. In the subjects who underwent DC the ICP was 36,4 mmHg immediately before and 12,6 mmHg immediately after the DC. The ICP then levelled out at just above 20 mmHg. The ICP was significant lower during the 72 hours following DC. The outcome did not differ between subjects who had undergone DC or not. Subclinical electroencephalographic seizures and status epilepticus have been reported to be common in subjects treated for traumatic brain injury (TBI). This can negatively influence the outcome giving rise to secondary brain injuries. The occurrence of seizures in subjects treated for TBI using continuous EEG monitoring was therefore prospectively studied. During 7334 hours of EEG recording in 47 patients no electroencephalographic seizures were observed. Theoretically, and based on animal studies, prostacyclin (PGI2) can improve the microcirculation of the brain, decreasing the risk for secondary ischaemic brain injury. PGI2 was introduced to the treatment in a prospective randomised double blinded study (epoprostenol 0,5 ng/kg/min). The effect of PGI1 pkt was analysed using the lactate/pyruvate ratio (L/P) measured by cerebral microdialysis in order to study the energy metabolism in the brain. The outcome was measured as Glasgow Outcome Scale (GOS) at 3 months follow-up. Forty-eight subjects were included. The L/P was pathological high during the first day, thereafter decreasing. There was no significant difference in L/P or outcome between the treated and non-treated group. At 3 months the mortality was 12,5% (95,8% was discharged alive from the ICU), and favourable outcome (GOS 4-5) was 52%. In the same study the brain injury biomarkers S-100B and NSE were followed twice a day for five days to evaluate brain injury and investigate the possible use of these biomarkers for outcome prediction. Initially the biomarkers were elevated to pathological levels which decreased over time. The biomarkers were significant elevated in subjects with Glasgow Coma Scale 3 (GCS) and GOS 1 compared with subjects with GCS 4-8 and GOS 2–5, respectively. A correlation to outcome was found but this correlation could not be used to predict clinical outcome. It is concluded that the ICP measurements are valid and the treatment protocol is a safe and solid protocol, yielding among the best reported results in the world, in regard to favourable outcome as well as in regard to mortality. Epoprostenol in the given dose was not shown to have any effects on the microdialysis parameters nor the clinical outcome. In sTBI L/P and brain injury biomarkers can not be used to predict the final outcome.

Includes vita. / Title from PDF of title page. / Document formatted into pages; contains 137 pages. / Thesis (Ph.D.)--University of South Florida, 2003. / Includes bibliographical references. / Text (Electronic thesis) in PDF format. / ABSTRACT: The researcher examined the relationships between tuberous sclerosis, a multi-system genetic disorder, and school functioning through the use of a parent questionnaire and behavior rating scale. Information was gathered on the typical school experiences of children with tuberous sclerosis, including educational placement and services, behavioral functioning, parent involvement, and parent satisfaction. The results indicated that the majority of students with tuberous sclerosis are in special education and receiving related services. Three-quarters received one or more related services through the public school, and 30% received private related services paid for by their parents. Parent involvement was positively correlated with parent satisfaction, and negatively correlated with t-scores on the Withdrawn/Depressed subscale of the CBCL. / ABSTRACT: Parents of children receiving Autism services were generally less satisfied with their children's school experiences than other parents. Parent satisfaction was negatively correlated with the Attention Problems scale of the CBCL. Student age was negatively correlated with time in inclusion and with related services. This information forms the basis for a discussion of school psychologists' roles in the educational success of students with tuberous sclerosis and the critical areas towards which interventions should be directed. / System requirements: World Wide Web browser and PDF reader. / Mode of access: World Wide Web.

Nors Rolando epilepsijai (RE) būdinga gerybinė eiga, dalis sergančiųjų turi miego bei elgesio problemų. Nuosekliai ištyrėme 75 RE sergančius bei 32 lyginamosios grupės nesergančius epilepsija pacientus. Grupės nesiskyrė pagal amžių ir lytį. Suskirstėme sergančiuosius RE į dvi grupes pagal priepuolių pasikartojimą per paskutinius 6 mėnesius. Elgesio sutrikimai buvo vertinami pasitelkiant CBCL (angl. Child Behavior Checklist) klausimyną, miego sutrikimai-pagal vaikų miego sutrikimų skalę (SDSC) (angl. Sleep Disturbance Scale for Children). Vertinome miego ir elgesio problemas bei jų sąsajas su klinikiniais, EEG duomenimis bei vizualinės-motorinės reakcijos laiko duomenimis. Bendradarbiaudami su Vilniaus universiteto Matematikos ir informatikos fakultetu rankiniu ir automatiniu būdu tyrėme pacientų EEG. Tik tiems RE pacientams, kuriems buvo priepuolių per paskutinius 6 mėn., nustatėme patikimai aukštesnius SDSC klausimyno įverčius (padidintą mieguistumą, kvėpavimo sutrikimus miego metu, miego- budrumo ritmo sutrikimus, ilgesnę miego latenciją) bei patikimai aukštesnius CBCL klausimyno rodiklius (socialinių sunkumų, mąstymo sunkumų, dėmesio sunkumų, agresyvaus elgesio bei bendrų sunkumų skalių įverčius). Elgesio problemos buvo susiję su ilgesne epilepsijos trukme, sunkesniais ir dažnesniais priepuoliais, miego problemomis bei miego EEG pakitimais (>35/min pikų kiekiu bei pikų lokalizacija ir kitose nei centrotemporalinės srityse). Budrumo EEG nustatytas >17/min pikų kiekis buvo... [toliau žr. visą tekstą] / Although patients with benign Rolandic epilepsy (RE) exhibit a benign course of the disease, some of them display sleep and behavioural problems. Seventy five patients with RE, aged 6–11 years, were included in this study. The patients were divided into two subgroups according to the presence of seizures over the preceding 6 months. The comparison group comprised 32 patients without epilepsy and with similar characteristics in terms of age and sex. All patients underwent evaluation of sleep (Sleep Disturbance Scale for Children) and behaviour (Lithuanian version of Child Behaviour Checklist). We examined the sleep and behavioural problems in correlation with the clinical data, EEG data, and simple visual-motor reaction time data. We automatically and manually analysed EEG in the collaboration with Vilnius University Faculty of Mathematics and Informatics. Only patients who had had seizures over the preceding 6 months displayed significantly higher scores for sleep problems (disorders of excessive daytime sleepiness, disorders of sleep breathing, and disorders of sleep-wake transition, longer sleep onset latency), and behavioural problems (social problems, thought problems, attention problems, and aggressive behavior) than the patients of the comparison group. Behavioural problems were associated with the longer epilepsy duration, more frequent and more severe seizures, sleep problems and sleep EEG data (spike frequency >35/min and spike focus, spreading to the other than... [to full text]

Nors Rolando epilepsijai (RE) būdinga gerybinė eiga, dalis sergančiųjų turi miego bei elgesio problemų. Nuosekliai ištyrėme 75 RE sergančius bei 32 lyginamosios grupės nesergančius epilepsija pacientus. Grupės nesiskyrė pagal amžių ir lytį. Suskirstėme sergančiuosius RE į dvi grupes pagal priepuolių pasikartojimą per paskutinius 6 mėnesius. Elgesio sutrikimai buvo vertinami pasitelkiant CBCL (angl. Child Behavior Checklist) klausimyną, miego sutrikimai-pagal vaikų miego sutrikimų skalę (SDSC) (angl. Sleep Disturbance Scale for Children). Vertinome miego ir elgesio problemas bei jų sąsajas su klinikiniais, EEG duomenimis bei vizualinės-motorinės reakcijos laiko duomenimis. Bendradarbiaudami su Vilniaus universiteto Matematikos ir informatikos fakultetu rankiniu ir automatiniu būdu tyrėme pacientų EEG. Tik tiems RE pacientams, kuriems buvo priepuolių per paskutinius 6 mėn., nustatėme patikimai aukštesnius SDSC klausimyno įverčius (padidintą mieguistumą, kvėpavimo sutrikimus miego metu, miego- budrumo ritmo sutrikimus, ilgesnę miego latenciją) bei patikimai aukštesnius CBCL klausimyno rodiklius (socialinių sunkumų, mąstymo sunkumų, dėmesio sunkumų, agresyvaus elgesio bei bendrų sunkumų skalių įverčius). Elgesio problemos buvo susiję su ilgesne epilepsijos trukme, sunkesniais ir dažnesniais priepuoliais, miego problemomis bei miego EEG pakitimais (>35/min pikų kiekiu bei pikų lokalizacija ir kitose nei centrotemporalinės srityse). Budrumo EEG nustatytas >17/min pikų kiekis buvo... [toliau žr. visą tekstą] / Although patients with benign Rolandic epilepsy (RE) exhibit a benign course of the disease, some of them display sleep and behavioural problems. Seventy five patients with RE, aged 6–11 years, were included in this study. The patients were divided into two subgroups according to the presence of seizures over the preceding 6 months. The comparison group comprised 32 patients without epilepsy and with similar characteristics in terms of age and sex. All patients underwent evaluation of sleep (Sleep Disturbance Scale for Children) and behaviour (Lithuanian version of Child Behaviour Checklist). We examined the sleep and behavioural problems in correlation with the clinical data, EEG data, and simple visual-motor reaction time data. We automatically and manually analysed EEG in the collaboration with Vilnius University Faculty of Mathematics and Informatics. Only patients who had had seizures over the preceding 6 months displayed significantly higher scores for sleep problems (disorders of excessive daytime sleepiness, disorders of sleep breathing, and disorders of sleep-wake transition, longer sleep onset latency), and behavioural problems (social problems, thought problems, attention problems, and aggressive behavior) than the patients of the comparison group. Behavioural problems were associated with the longer epilepsy duration, more frequent and more severe seizures, sleep problems and sleep EEG data (spike frequency >35/min and spike focus, spreading to the other than... [to full text]

Techn. Universiẗat, Diss., 2006--Dresden.

Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisors: Maxwell B. Stinchcombe and R. Preston McAfee. Vita. Includes bibliographical references.

Danos mitocondriais e diminuição nos níveis de ATP têm sido recentemente atribuídos à sertralina. Nós investigamos o efeito da sertralina em plaquetas humanas, já que estas células demonstram várias similaridades funcionais com neurônios e astrócitos. Os efeitos da sertralina em diferentes parâmetros foram investigados em plaquetas lavadas de 18 voluntários saudáveis do sexo masculino, depois de 24h de exposição ao fármaco. A toxicidade da sertralina foi observada apenas nas concentrações mais altas, 30 e 100μM, nas quais a viabilidade das plaquetas foi significantemente reduzida a 76 ± 3% e 20 ± 2 %, respectivamente. As mesmas concentrações reduziram o ATP a 73 ± 3% e 13 ± 2 %, respectivamente. Os valores basais de glicogênio não foram afetados significativamente pelo tratamento com sertralina. A captação de glutamato foi reduzida de forma significativa após o tratamento com 3, 30 e 100 μM, em 28 ± 6 %, 32 ± 5% e 54 ± 4 %, respectivamente. Nosso estudo demonstra que a sertralina em concentrações terapêuticas não compromete a viabilidade plaquetária, porém seu uso não pode ser considerado isento de risco, pois em um cenário no qual os níveis de glutamato extracelular estão potencialmente aumentados, a sertralina poderia agravar uma condição excitotóxica / Mitochondrial damage and fall in ATP levels have been recently attributed to sertraline. We investigated the effect of sertraline on human platelets, since these cells display several functional similarities with neurons and astrocytes. The effects of sertraline on different parameters were investigated in washed platelets from 18 healthy male volunteers, after 24 h of drug exposure. Sertraline toxicity was observed only at the highest concentrations, 30 and 100 μM, which significantly reduced platelet viability to 76 ± 3% and 20 ± 2%, respectively. The same concentrations significantly decreased total ATP to 73 ± 3% and 13 ± 2%, respectively. Basal values of glycogen were not significantly affected by sertraline treatment. The glutamate uptake was significantly reduced after treatment with 3, 30 and 100 μM, by 28 ± 6%, 32 ± 5% and 54 ± 4%, respectively. Our study showed that sertraline at therapeutic concentrations does not compromise platelet viability, but its use may not be without risk, since in a scenario where extracellular glutamate levels are potentially increased, sertraline might aggravate an excitotoxic condition.

In die studie is besin oor die wyse waarop die opvoedkundige sielkundige die probleme wat deur die epileptiese adolessente dogter ondervind word, kan aanspreek. 'n Groepterapeutiese begeleidingsprogram is ten doel gestel sodat die intra- en inter-psigiese effek daarvan op die epileptiese adolessente dogter bepaal kon word. Agt epileptiese adolessente dogters is vir die doel van hierdie studie geselekteer, waama hulle aan 'n reeks psigometriese toetse onderwerp is. Voorts het die onderskeie vakonderwysers 'n evalueringsvraelys voltooi. Daar is bevind dat die dogters in die algemeen 'n negatiewe selfkonsep het. Die meeste proefpersone was angstig en depressief. Verhoudingsprobleme en 'n gebrek aan doelstellings het ook onder hulle voorgekom. Volgens die literatuurstudie blyk dit 66k dat die epileptiese kind oor die algemeen 'n hoe voorkoms van psigo-sosiale probleme toon en <lat <lit dikwels lei tot 'n negatiewe selfkonsep. Faktore wat die psigo-sosiale wording van die epileptiese kind kan bei'nvloed, is neuroIV biologiese faktore (byvoorbeeld die tipe toeval), psigo-sosiale faktore (byvoorbeeld stigmatisering) en farmakologiese faktore (byvoorbeeld newe-effekte van medikasie ). Die proefpersone is onderwerp aan 'n groepterapeutiese begeleidingsprogram van tien sessies. Die program het gefokus op intra-psigiese aspekte soos die regstelling van negatiewe selfspraak, asook op die bewusmaking en oefening van interpersoonlike vaardighede. Uit die resultate van die na-toetsing kan afgelei word dat persoonlike groei plaasgevind het - in die algemeen was daar 'n verhoging ten opsigte van die selfkonsep. Interpersoonlike verhoudinge het skynbaar ook verbeter, terwyl angs afgeneem en depressie verminder het. Voorts het die proefpersone se skolastiese prestasie met 5,1 persent verbeter. Die groepterapeutiese begeleidingsprogram het dus 'n positiewe intra- en inter-psigiese effek op die epileptiese adolessente dogter gehad. / This study addressed the ways in which the educational psychologist can address the problems experienced by epileptic adolescent girls. Eight epileptic adolescent girls were subjected to a series of psychometric tests. The results indicated that in general, the majority of the girls were suffering from a negative self-concept, depression and anxiety. They experienced problems with relationships and reflected a lack of goals. The epileptic adolescent girls were subjected to a group therapeutic guidance programme. The programme focussed on intra- and interpsychological aspects such as the modification of negative self-talk and the exercising of interpersonal skills. The results of follow-up tests revealed that in general there had been an improvement of the self-concept. Apparently inter-personal relations had improved, whereas anxiety and depression had declined. At the same time, school performance had increased. Hence, the group therapeutic guidance programme had a positive intra- and interpsychological effect on the epileptic adolescent girls. / Psychology of Education / M.Ed.