Orientadores: Fernando Ferreira Costa, Anderson Ferreira da Cunha / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-22T17:06:48Z (GMT). No. of bitstreams: 1 Branco_DianaSantos_D.pdf: 12860329 bytes, checksum: 5c5da209d2a41c9596907283c3c9e5e8 (MD5) Previous issue date: 2013 / Resumo: Os mecanismos moleculares envolvidos no perfil de expressão durante a eritropoese tem sido objeto de numerosas investigações como, por exemplo, o estudo da regulação gênica em células de linhagem eritroide. Esses estudos permitem a identificação de novos genes com potencial participação nesse processo e, adicionalmente, possibilitam um melhor entendimento dos genes já identificados na maturação das células eritroides e que possam estar envolvidos na produção de hemoglobinas. Nosso grupo de pesquisa identificou os diversos genes diferencialmente expressos durante a eritropoese. Dentre eles, os fatores de transcrição, EYA3 e HES6 e a latexina, LX, apresentaram maior expressão na fase final da eritropoese in vitro. Nossos dados sugerem participação dos genes EYA3 e LX, nas fases intermediaria e final da diferenciação eritroide, na expressão dos genes das globinas alfa e gama e na produção de HbF in vitro. Adicionalmente, no modelo in vivo zebrafish, os genes eya1, eya2, eya3, eya4, hes6 e hes13 apresentaram padrão de expressão ubíquo, enquanto que o gene lxn apresentou expressão especifica na ICM, tornando-o o candidato mais promissor para ser silenciado. O silenciamento desse gene em zebrafish apresentou fenótipo anêmico em embriões 72hpf, mas não em 48hpf, sugerindo que a anemia e decorrente de um processo no final da diferenciação eritroide, corroborando os dados encontrados em cultura in vitro. Estudos adicionais são necessários para compreensão dos mecanismos e vias envolvidos na participação do gene LX, durante o processo de diferenciação eritroide. Outros genes com potencial participação no processo de eritropoese são CLPX, TRAK2 E GFI. O gene CLPX codifica a caseinolytic peptidase X, uma proteína xvii altamente conservada durante a evolução e que apresenta função de chaperona dependente de ATP. Os dados deste trabalho mostram que o silenciamento do gene clpx1 reduziu significativamente os níveis de hemoglobinizacao e produção de eritrócitos em zebrafish. Contudo, estudos adicionais para o gene clpx2 precisam ser realizados para melhor compreender a possível função desses genes na produção de Heme. O gene TRAK2, por sua vez, e uma Trafficking Protein, Kinesin-Binding 2, envolvida no movimento da mitocôndria ao longo dos microtubulos. Os resultados obtidos em colaboração com o pesquisador Jeffrey Miller, M.D. (NIH/NIDDK) mostraram envolvimento desse gene na eritropoese em modelo in vitro de cultura primaria. No presente estudo, dentre os ortologos para o gene TRAK2 humano avaliados, apenas o trak1.1 parece ter sua função conservada nos teleósteos. O silenciamento desse gene gerou fenótipo anêmico nos embriões avaliados, corroborando os dados obtidos originalmente em cultura de células primaria. Finalmente, os fatores de transcrição de zebrafish gfi1aa, gfi1ab e gfi1b, ortologos aos fatores de transcrição da família Grow Factor Independence (GFI) em humanos também tiveram seu papel avaliado na hematopoese. Nossos dados mostram participação de gf1aa fase inicial de hematopoese e de gf1b na hematopoese definitiva. Também foi determinada a relação epistática entre os fatores gfi e os fatores de transcrição chave hematopoiéticos, mostrando que gfi1aa e gfi1b, juntamente com lmo2, scl, runx-1 e c-myb atuam como reguladores de HSPC em teleósteos / Abstract: Molecular mechanisms involved in expression profile during erythropoiesis have been the subject of numerous investigations such study of gene regulation in erythroid cell culture. These studies allow us to identify new genes potentially involved in erythroid differentiation and additionally to investigate genes already known as regulators of red blood cells and hemoglobin production. Our research group identified several genes differentially expressed during erythropoiesis. Among them, the transcription factors, EYA3 and HES6 and the latexin, LX, were found to have higher expression in the final phase of the in vitro erythropoiesis. Our data suggest that EYA3 and LX, are involved in the intermediate and final stages of erythropoiesis, expression pattern of alfa and gama globin and HbF production in vitro. Additionally in zebrafish model, eya1, eya2, eya3, eya4, hes6 and hes13 showed a ubiquitous expression pattern, while lxn showed specific expression in the ICM, making it the most promising candidate to be knockdowned. lxn knockdown in zebrafish showed anemic phenotype at 72hpf embryos, but not at 48hpf, suggesting that the anemia results is due to a process in the end of the erythroid differentiation, corroborating the results found for in vitro cultures. Additional studies are necessary to understand the mechanisms and pathways involved in the participation of the LX, gene during the process of erythroid differentiation. CLPX, TRAK2 and GFI transcription factors are also potentially candidates to be involved in erythropoiesis. CLPX gene codes for caseinolytic peptidase X, a protein highly conserved during evolution, which presents an ATP-dependent chaperone function. Data from this study showed that clpx1 knockdown reduced significantly hemoglobinization levels and erythroid production in zebrafish. However, future studies xv for the clpx2 gene is needed to better understand the function of these genes in the heme production. TRAK2 gene, in turn, is a Trafficking Protein, Kinesin-Binding 2, involved in mitochondrial movement along microtubules. Results obtained in collaboration with the researcher Jeffrey Miller, M.D. (NIH/NIDDK), showed the involvement of this gene in erythropoiesis in primary culture in vitro models. In this study, from the orthologs for the human TRAK2 gene analyzed, only trak1.1 appears to have its function conserved in teleosts. The silencing of this gene generated anemic phenotype in the embryos tested, corroborating the original results obtained in primary cell culture. Finally, gfi1aa, gfi1ab and gfi1b zebrafish transcription factors, orthologous to the Grow Factor Independence (GFI) family transcription factors in humans, also had their function evaluated in hematopoiesis. Our data suggest is involved in the initial phase of hematopoiesis while gf1b has a role in the definite hematopoiesis. The epistatic relation between the gfi and the hematopoietic key transcription factors was also determined, showing that gfi1aa and gfi1b, together with lmo2, scl, runx-1 and c-myb also act as regulators of HSPC in teleosts / Doutorado / Genetica Vegetal e Melhoramento / Doutora em Genética e Biologia Molecular

This work focuses on the design circularly polarized serial antennas array operate in the ISM band (5.725 GHz to 5.875 GHz). Design of antennas is divided into three parts. In the first part is designed feeding system, followed by two types of circularly polarized antennas and at the last point, these components are integrated. Furthermore, these antennas are fabricated, measured their parameters and used for experimental verification of the influence of multipath transmission in-vehicle.

The Swedish Defence Materiel Administration provides tests and evaluations of military Aircrafts and their systems as well as provide services in connection with military exercises. Testing aircraft against a radar antenna and training crews with this radar is part of that offering. The radar is deployed in a container rig and controlled by a computer running Windows 2000. The current option to control this computer is a mouse and keyboard. In this thesis, a system will be designed that is able to improve the ease of use of this rig while minimizing any need to modify the radar rig’s already established hardware and software. The resulting system designed used a commercially available joystick and off the shelf single board micro-controllers in combination with a graphical user interface to supply the radar rig with a converted input from the joystick in the form of mouse and keyboard commands, simplifying the end-user experience. / Försvarets materielverk tillhandahåller test och evaluering av militära flygsystem. De tillhandahåller även tjänster rörande militära övningar såsom belysning av flygplan med en radar för att öva piloter. Denna radar är monterad i en container och kontrolleras av en styrdator som kör operativsystemet Windows 2000. Denna dator styrs med hjälp av en mus och tangentbord. I denna rapport kommer ett system designas som kan förbättra användarupplevelsen av denna dator samtidigt som förändringar av hårdvara eller mjukvara i styrdatorn undviks. Resultatet av rapporten var ett system bestående av en kommersiellt tillgänglig joystick och två microkontrollers i kombination med ett grafiskt användargränssnitt som omvandlar knapptryck och styrutslag från joysticken till mus och tangentbords kommandon i styrdatorn.

Jitter requirements have become more stringent with higher speed serial communication links. Reducing jitter, with the main focus on reducing data dependant jitter (DDJ), is presented by employing adaptive finite impulse response (FIR) filter pre-emphasis. The adaptive FIR pre-emphasis is implemented in the IBM 7WL 0.18 µm SiGe BiCMOS process. SiGe heterojunction bipolar transistors (HBTs) provide high bandwidth, low noise devices which could reduce the total system jitter. The trade-offs between utilising metal oxide semiconductor (MOS) current mode logic (CML) and SiGe bipolar CML are also discussed in comparison with a very high fT (IBM 8HP process with fT = 200 GHz) process. A reduction in total system jitter can be achieved by keeping the sub-components of the system jitter constant while optimising the DDJ. High speed CML circuits have been employed to allow data rates in excess of 5 Gb/s to be transmitted whilst still maintaining an internal voltage swing of at least 300 mV. This allows the final FIR filter adaptation scheme to minimise the DDJ within 12.5 % of a unit interval, at a data rate of 5 Gb/s implementing 6 FIR pre-emphasis filter taps, for a worst case copper backplane channel (30" FR-4 channel). The implemented integrated circuit (IC) designed as part of the verification process takes up less than 1 mm2 of silicon real estate. In this dissertation, SPICE simulation results are presented, as well as the novel IC implementation of the proposed FIR filter adaptation technique as part of the hypothesis verification procedure. The implemented transmitter and receiver were tested for functionality, and showed the successful functional behaviour of all the implemented CML gates associated with the first filter tap. However, due to the slow charge and discharge rate of the pulse generation circuit in both the transmitter and receiver, only the main operational state of the transmitter could be experimentally validated. As a result of the adaptation scheme implemented, the contribution in this research lies in that a designer utilising such an IC can optimise the DDJ, reducing the total system jitter, and hence increasing the data fidelity with minimal effort. / Dissertation (MEng)--University of Pretoria, 2011. / Electrical, Electronic and Computer Engineering / unrestricted

This research is a qualitative art-based study exploring trauma markers found in the artwork of serial killers through the participation of licensed art therapists trained in the identification and treatment of trauma. The literature review covers a broad range of variables that influence the development of mass murderers, and how art therapy may be useful in working with serial killer populations. The study offers a unique, non-verbal exploration of the trauma experienced by serial killers through the art therapy lens. The three participants provided invaluable data through their responses to a questionnaire and creation of response art that demonstrates evidence of complex trauma, military trauma and traumatic brain injury; all of which were supported by previous research found in the literature. Themes identified through the analysis of the data included: communication, representation, chaos and violence, life and death, and illusion of power. This study demonstrates the importance of understanding serial killers’ psychological makeup, the traumas and other experiences that have profound impacts on them, and the contexts in which they develop. This may lead to a better understanding in how to look for the warning signs in their development and artwork; allowing for the potential to prevent such violent behavior with early intervention.

ITC/USA 2006 Conference Proceedings / The Forty-Second Annual International Telemetering Conference and Technical Exhibition / October 23-26, 2006 / Town and Country Resort & Convention Center, San Diego, California / We describe the design and development of a baseband telemetry system for multistage launch vehicles. The system is organized as a three tier one with remote data acquisition and processing units and a centralized control unit. The front-end Data Acquisition Units (DAUs) feature software programmable amplification, offset, filtering and sensor excitation and thus are flexible to interface directly to a variety of sensors used in launch vehicles. The Data Processing Units (DPUs) gather data from DAUs through a serial link compatible to RS-485 standards and carry out a variety of data analysis and data compression functions on selected channels under software control. The central Telemetry Control Unit (TCU) receives this data through a transformer isolated link compatible to MIL-1553B standards and performs the functions of data delay, data storage, onboard computer data monitoring, PCM formatting and pre-modulation signal conditioning to achieve miniaturization. The configuration and features of this telemetry system make its integration simple without compromising on data integrity and reliability and suit the adoption of futuristic technologies and concepts such as smart sensor networks, adaptability, reconfiguration and vehicle health management.

ITC/USA 2008 Conference Proceedings / The Forty-Fourth Annual International Telemetering Conference and Technical Exhibition / October 27-30, 2008 / Town and Country Resort & Convention Center, San Diego, California / A previous paper was presented detailing the design and testing of the first networked demonstration system (ITC 2006) for iNET. This paper extends that work by testing a commercial off the shelf (COTS) solution for the wireless network connection of the Telemetry Network System (TmNS). This paper will briefly discuss specific pieces of the airborne and ground station system but will concentrate on the new wireless network link, how it was tested, and how well it performed. Flight testing results will be presented accessing the performance of the wireless network link.

This thesis aims to investigate how well associative learning can account for human sequence learning under incidental conditions. It seems that we can learn complex sequential information about events in our environment, for example language or music, incidentally, without being aware of it. Awareness is, however, a complex issue with arguments for (Dienes, 2012) and against (Shanks, 2005) the existence of implicit learning processes. A dual process account proposes that there exist two different learning systems, one based on conscious, controlled reasoning and rules, and the other based on automatic association formation, which can take place outside of awareness (McLaren, Green, & Mackintosh, 1994). This thesis attempts to use the predictions of an associative account in conjunction with a suitable method for investigating implicit learning: sequence learning (Destrebecqz & Cleeremans, 2003). The research involves a collection of serial reaction time (SRT) tasks whereby participants respond to on-screen stimuli that follow a sequence that they were (intentional learning) or were not (incidental learning) informed of. Following on from the experimental design of Jones and McLaren (2009) this thesis provides evidence that humans differ in their ability to learn different sequential contingencies. After training sequences of trials where the current trial location was twice as likely to be either: the same as (Same rule); or different to (Different rule) the location two trials before this, participants were far better at learning the latter rule. I found that this result was not adequately simulated by the benchmark associative model of sequence learning, the Augmented SRN (Cleeremans & McClelland, 1991), and present a revised model. This model, amongst other attributes, represents all the stimuli experienced by participants and can therefore learn stimulus-response contingencies. These seem to block learning (to some extent) about the Same rule thus providing an associative explanation of the advantage for acquisition of the Different rule. Further predictions regarding the role of additional stimuli alongside sequence learning were then derived from this associative account and tested on human participants. The first of these was that additional stimuli within the task will interact with sequence learning. I found that human participants show increased Same rule learning when additional, concurrently presented stimuli follow the previous element in the sequence. I demonstrate that when participants perform an SRT task where responses are predicted by the colour of a cue, they are able to learn about this relationship in the absence of awareness. Using this cue-response learning I further investigate cue-competition between sequences and colours under incidental conditions and find evidence that suggests between cue associations may alter the influence of cue competition. These results altogether suggest that stimuli – both simple and sequential – can be learned under incidental conditions. This thesis further proposes that learning about simple and more complex relationships between stimuli interacts according to the predictions of an associative account and provides evidence that contributes to a dual process understanding of human learning.

This study tests for the market efficiency of the Nairobi Securities Exchange (NSE) after the year 2000 to determine the effect of technological advancements on market efficiency. Data that is used is the NSE 20 share index over the period 2001 to 2015; and the NSE All Share Index (NSE ASI) from its initiation during 2008 to 2015. We cannot accept the Efficient Market Hypothesis (EMH) for the NSE using the serial correlation test, the unit root tests and the runs test. However, we can accept the EMH for the more robust variance ratio test. Overall, the results of the market efficiency are mixed. The most significant finding is that the efficiency of the NSE has increased since the year 2000 which suggests that advancements in technology have contributed to the increase in the market efficiency of the NSE. / Business Management / M. Com. (Business Management)

International Telemetering Conference Proceedings / October 17-20, 1994 / Town & Country Hotel and Conference Center, San Diego, California / The Data Acquisition, Analysis, and Simulation System (DAAS) is a computer system designed to allow data sources on spacecraft in the Flight System Testbed (FST) to be monitored, analyzed, and simulated. This system will be used primarily by personnel in the Flight System Testbed, flight project designers, and test engineers to investigate new technology that may prove useful across many flight projects. Furthermore, it will be used to test various spacecraft design possibilities during prototyping. The basic capabilities of the DAAS involve unobtrusively monitoring various information sources on a developing spacecraft. This system also provides the capability to generate simulated data in appropriate formats at a given data rate, and to inject this data onto the communication line or bus, using the necessary communication protocol. The DAAS involves Serial RS232/RS422, Ethernet, and MIL-STD-1553 communication protocols, as well as LabVIEW software, VME hardware, and SunOS/UNIX operating systems.