abstract: Over the last century, X-ray crystallography has been established as the most successful technique for unravelling the structure-function relationship in molecules. For integral membrane proteins, growing well-ordered large crystals is a challenge and hence, there is room for improving current methods of macromolecular crystallography and for exploring complimentary techniques. Since protein function is deeply associated with its structural dynamics, static position of atoms in a macromolecule are insufficient to unlock the mechanism. The availability of X-ray free electron lasers presents an opportunity to study micron-sized crystals that could be triggered (using light, small molecules or physical conditions) to capture macromolecules in action. This method of ‘Time-resolved serial crystallography’ answers key biological questions by capturing snapshots of conformational changes associated with multi-step reactions. This dissertation describes approaches for studying structures of large membrane protein complexes. Both macro and micro-seeding techniques have been implemented for improving crystal quality and obtaining high-resolution structures. Well-diffracting 15-20 micron crystals of active Photosystem II were used to perform time-resolved studies with fixed-target Roadrunner sample delivery system. By employing continuous diffraction obtained up to 2 A, significant progress can be made towards understanding the process of water oxidation. Structure of Photosystem I was solved to 2.3 A by X-ray crystallography and to medium resolution of 4.8 A using Cryogenic electron microscopy. Using complimentary techniques to study macromolecules provides an insight into differences among methods in structural biology. This helps in overcoming limitations of one specific technique and contributes in greater knowledge of the molecule under study. / Dissertation/Thesis / Doctoral Dissertation Biochemistry 2018

Este artículo analiza las construcciones verbales seriales (CVS) del mapudungun, lengua indígena hablada en el sur de la República Argentina, sobre todo en las provincias de La Pampa, Neuquén, Río Negro y Chubut. Los estudios tradicionales del mapudungun han tratado los verbos conformadospor dos o más raíces verbales como lexemas compuestos. A partir de la perspectiva tipológica, que define las construcciones verbales seriales como una secuencia de verbos que funciona como un predicado único, sin presentar elementos coordinantes ni subordinantes entre ellos, se las analiza estableciendo los distintos tipos de CVS, sus valores semánticos, así como su frecuencia de aparición en los corpora sobre los que se basanuestro trabajo. -- This article analyzes the serial verb constructions (SVC) of Mapudungun, an indigenous language spoken in the southern region of Argentina —mainly in the provinces of La Pampa, Neuquén, Río Negro y Chubut.Traditional investigations of Mapudungun have considered verbs constituted by two or more verbal roots as complex lexemes. From a typological trend, which defines SVC as a sequence of verbs that functions as a unique predicate, without presenting coordination or subordination elements betweenthem, we analyze Mapudungun SVC and, thus, establish a typology, their semantic values and the appearance frequency in the corpora we gathered for this investigation.

abstract: This dissertation examines the discursive construction of the trope of the ideal girl in popular American girls' series in the twentieth century. Girls' cultural artifacts, including girls' literature series, provide sites for understanding girls' experiences and exploring girlhood itself as a socially constructed identity, yet are often overlooked due to their presumed insignificance. Simple dismissal of these texts ignores the weight of their popularity and the processes through which they reach such status. This project challenges the derisive attitude towards girls' culture and begins with the assumption that these cultural texts do ideological work and therefore require consideration. The dissertation traces the development of the ideal and non-ideal girl over time, taking into account the cultural, political, and economic factors that facilitate the production of the discourses of girlhood. I include analysis of texts from six popular American girls' series as primary texts; visual elements or media productions related to the series; and supporting historical documents such as newspapers, "expert" texts, popular parents' and girls' magazines, film; and advertising. Methodological approach incorporates elements of literary criticism and discourse analysis, combining literary, historical, and cultural approaches to primary texts and supporting documents to trace the moments of production, resistance, and response in the figure of the ideal girl. Throughout the project, I pay particular attention to the intersections of race, class, gender, sexuality, and nationality in the figure of the ideal girl and her non-ideal counterparts. I argue that girls' series, slipping under the radar as a denigrated cultural medium, capture and perpetuate cultural anxieties around heterosexuality, whiteness and American identity, appropriate gender roles, and class mobility. These texts discipline the non-ideal girl toward the ideal, always with the expectation of failure. / Dissertation/Thesis / Ph.D. Gender Studies 2013

Orientador: Jaime Portugheis / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica e de Computação / Made available in DSpace on 2018-08-06T08:08:11Z (GMT). No. of bitstreams: 1 Sharma_Manish_M.pdf: 1001397 bytes, checksum: 04250e6b88e19bb784d3b68313ace258 (MD5) Previous issue date: 2006 / Resumo: Nesta dissertação abordamos o problema de como construir codificadores bit-geometricamente uniformes (BGU) para a utilização como codificadores internos em sistemas com concatenação serial de códigos. A utilização destes codificadores implica na facilidade de determinação de parâmetros necessários para a análise do desempenho dos sistemas. Há um grande controle sobre estes parâmetros no projeto destes codificadores utilizando o método descrito neste trabalho, o que sugere que bons codificadores e conseqüentemente bons sistemas podem ser obtidos desta maneira. Além disso, os códigos gerados por estes codificadores possuem a propriedade de uniformidade de erro de bit, o que facilita bastante sua análise / Abstract: This thesis approaches the problem of building bit-geometrically uniform (BGU) encoders to be used as inner encoders in systems with serially concatenated codes. By using this type of encoders, certain parameters that are used to analyze the system's performance are easily determined. There is a great control over these parameters when building encoders using the method described in this work, suggesting that good encoders and subsequently good systems can be obtained. Besides, the codes generated by these encoders posses the uniform bit error property, that greatly facilitates their analysis / Mestrado / Engenharia de Telecomunicações / Mestre em Engenharia Elétrica

Submitted by Erika Demachki (erikademachki@gmail.com) on 2016-08-04T17:58:59Z No. of bitstreams: 2 Dissertação - Victor Hugo de Carvalho Caldas - 2016.pdf: 1875779 bytes, checksum: 16e88aef5001741aa908ab366c41da8b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2016-08-04T18:04:43Z (GMT) No. of bitstreams: 2 Dissertação - Victor Hugo de Carvalho Caldas - 2016.pdf: 1875779 bytes, checksum: 16e88aef5001741aa908ab366c41da8b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-08-04T18:04:43Z (GMT). No. of bitstreams: 2 Dissertação - Victor Hugo de Carvalho Caldas - 2016.pdf: 1875779 bytes, checksum: 16e88aef5001741aa908ab366c41da8b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-06-27 / In this work we investigate the construction of the character of the journalist in HBO series The Newsroom, comparing it with its cinematographic representation. In addition, we present an overview of the emergence and evolution of the TV series in USA, very popular today and available to the public through a variety of channels, which implies the importance of their understanding. / Neste trabalho investigamos a construção da personagem do jornalista em The Newsroom, série de TV do canal norte-americano HBO, comparando-a com a representação desse profissional no cinema. Além disso, apresentamos um panorama do surgimento e evolução das próprias séries televisivas nos Estados Unidos, hoje muito populares e disponíveis para o público por meio de uma variedade de canais, o que implica na importância do seu entendimento.

No procedimento de discriminação condicional de origem skinneriana, geralmente, usa-se o procedimento de matching-to-sample (MTS). Esse procedimento também é utilizado para se estudar a equivalência de estímulos. Sidman e Tailby (1982) estabeleceram três testes para verificar se estímulos usados em tarefas de discriminação condicional podem ser considerados equivalentes entre si: reflexividade, simetria e transitividade. No procedimento de discriminação condicional de origem pavloviana, os estímulos podem ser apresentados de duas formas: simultânea e seriada. Na apresentação simultânea, os estímulos são apresentados juntos ou sucessivamente, sem intervalo entre suas apresentações (XA). Na apresentação seriada há um intervalo entre as apresentações dos estímulos (XA). Dependendo da forma de apresentação dos estímulos, diferentes associações entre os estímulos e o estímulo incondicionado (US) são formadas. Quando a apresentação é simultânea, forma-se uma associação entre o estímulo X e o US. Quando a apresentação é seriada, forma-se uma associação entre o estímulo A e o US e o estímulo X estabelece a ocasião para a ocorrência da relação entre A e US ocorre (Ross & Holland, 1981). Na discriminação condicional de origem pavloviana, a liberação do US não depende do comportamento do rato. Entretanto, o procedimento também pode ser usado com comportamento operante. No presente trabalho, buscou-se estudar o efeito da apresentação seriada dos estímulos sobre o desempenho de ratos durante os testes de equivalência de estímulos em um procedimento de discriminação condicional. Foram utilizados 32 ratos machos Wistar. O experimento envolveu diversas fases: (a) treino ao bebedouro, (b) modelagem do comportamento de pressão à barra, (c) CRF, (d) discriminação simples, (e) discriminação condicional, (f) testes de transferência e os de equivalência de estímulos (reflexividade, simetria e transitividade). Os ratos foram divididos em 2 grupos: o Simultâneo e o Seriado. No grupo Simultâneo, os estímulos foram apresentados sucessivamente, não havendo intervalo de tempo entre suas apresentações, enquanto que no grupo Seriado, houve um intervalo de 5 segundos entre as apresentações dos estímulos. Cada um desses grupos foram subdivididos em 2 grupos: grupo Tom e grupo Luz. No primeiro grupo, o estímulo característica usado foi um Tom, enquanto que no segundo grupo o estímulo foi uma Luz. Os resultados mostraram que os ratos aprenderam a tarefa de discriminação condicional. No teste de transferência observou-se que houve transferência em praticamente todos os 4 grupos. Durante o teste de equivalência, foi observadas a emergência de algumas relações. Na discussão, os dados foram discutidos a partir das literaturas de discriminação condicional de origem skinneriana e de origem pavloviana. / In a Skinnerian conditional discrimination procedure generally the procedure of matching-to-sample (MTS) is used. This procedure is also used to study the stimulus equivalence. Sidman and Tailby (1982) established three tests to verify whether stimuli used in conditional discrimination tasks can be considered equivalent to each other: reflexivity, symmetry and transitivity. In a Pavlovian conditional discrimination procedure, the compounds of stimuli may be in two forms: simultaneous and serial. In the simultaneous compounds, the stimuli are presented together or successively, without an interval between their presentations (XA). In the serial compounds there is an interval between stimuli presentations (X A). Depending on the form of presentation of the compounds of stimuli, different associations between stimuli and the unconditioned stimulus (US) are formed. In a simultaneous compound of stimuli an association between stimulus X and the US is formed. In a serial compound of stimuli an association between the stimulus A and the US is formed and the stimulus X sets the occasion for the occurrence of the association between stimulus A and the US (Ross & Holland, 1981). In Pavlovian conditional discrimination, the US presentation does not depend on the behavior of the rat. However, the procedure can also be used with operant behavior. In this study, we sought to study the effect of serial presentation of stimuli on the performance of rats during tests of equivalence of stimuli in a conditional discrimination procedure. We used 32 male Wistar rats. The rats were divided into two groups: Simultaneous Compounds and Serial Compounds. In the Simultaneous Compounds Group, stimuli were presented successively with no interval between their presentations. In the Serial Compounds Group, there was an interval of 5 seconds between the presentations of stimuli. Each of these groups was subdivided into two groups: Tone and Light. In the Tone Group, the feature stimulus used was a tone, and in the Light Group the feature stimulus was a light. The results of the experiment showed that the rats learned the task of conditional discrimination. In the transfer test was observed substantial transfer in almost all four groups. During the equivalence test were observed emergence of certain relationships. In the discussion, the data were discussed using the literature from Skinnerian conditional discrimination and from Pavlovian conditional discrimination.

With the vigorous development of Internet of things, IoT gateways as the communication bridge of the whole IoT system has attracted more and more people’s attention. So the objective of this project is to measure and evaluate the performance of two kinds of IoT gateways including Arduino Uno and Vinnter IoT gateway. In order to reach this objective, the concrete implementation is to create a demonstrator via JAVA program to establish serial communication and measure the latency and throughput of respective IoT gateways. The measurement is implemented under a specific scenario which is to control the action of an actuator such as a LED connecting with IoT gateways via GPIO pins. Finally, the results will be presented via statistical data and line charts. Through analyzing the results, the performance of these two IoT gateways can be compared in terms of latency and throughput easily. Besides implementing the measurement in practice, this paper also involves the scientific research of many different industrial-level IoT gateways and their performance are compared on both hardware and software sides. Consequently, this thesis will show you the comparison of various IoT gateways both in theory and in practice.

The Internet of Things is going to bring the Internet into every objectsaround us. To enable this ambitious idea, tiny devices have to be connected within the global Internet. Such devices are extreme small so it’sbecoming a challenge to connect it to the Internet via TCP/IP. The thesispresents a way of connecting microcontrollers with other devices to jointlyform a distributed network.The thesis investigates and takes advantage of Internet of Things platform to implement the connection. SensibleThings is used as the platform. Limited by the hardware, microcontroller can’t run such a bloatedplatform. The thesis investigates different microcontrollers characteristics and chooses Arduino as a representative in the work. Then it realizes a bridge connection between Arduino and SensibleThings. Arduinois connected with a single-board computer, Raspberry Pi by a USB cable.SensibleThings is running on Raspberry Pi to process the network messages. The channel throughput, latency and general usability are measured and interoperated. As a result, the data indicates this is a promising, flexible, cost effective network topology. Microcontroller can join adistributed network by the bridge. Comparing to dedicate hardware solution, the bridge connection cuts down the implementation difficultiesand cost. The thesis also covers possible problems in such connection andproposes future work.

Context. According to Swedish National Council for Crime Prevention, there is an increase of 19% in residential burglary crimes in Sweden over the last decade and only 5% of the total crimes reported were actually solved by the law enforcement agencies. In order to solve these cases quickly and efficiently, the law enforcement agencies has to look into the possible linked serial crimes. Many studies have suggested to link crimes based on Modus Operendi and other characteristic. Sometimes crimes which are not possible to travel spatially with in the reported times but have similar Modus Operendi are also grouped as linked crimes. Investigating such crimes could possibly waste the resources of the law enforcement agencies. Objectives. In this study, we investigate the possibility of the usage of travel distance and travel duration between different crime locations while linking the residential burglary crimes. A filtering method has been designed and implemented for filtering the unlinked crimes from the estimated linked crimes by utilizing the distance and duration values. Methods. The objectives in this study are satisfied by conducting an experiment. The travel distance and travel duration values are obtained from various online direction services. The filtering method was first validated on ground truth represented by known linked crime series and then it was used to filter out crimes from the estimated linked crimes. Results. The filtering method had removed a total of 4% unlinked crimes from the estimated linked crime series when the travel mode is considered as driving. Whereas it had removed a total of 23% unlinked crimes from the estimated linked crime series when the travel mode is considered as walking. Also it was found that a burglar can take an average of 900 seconds (15 minutes) for committing a burglary. Conclusions. From this study it is evident that the usage of spatial and temporal values in linking residential burglaries gives effective crime links in a series. Also, the usage of Google Maps for getting distance and duration values can increase the overall performance of the filtering method in linking crimes.

Something that has recently gained popularity in the leading car manufacturing companies is the integration of an auto-reverse assistance system to improve the customer experience. The undeniable spread of smartphones and their significant role in human life in recent years, gave rise to the idea of designing an application to be used for reverse driving. As a result, the car company Volvo proposed an idea for this project: to implement an Android based application to facilitate reverse navigation in their trucks. From a technical point of view, the most crucial obstacle that should be addressed is implementing a secure and reliable communication link between the smartphone and the truck's control centre. Hence, the primary goal of this thesis work is to provide a secure channel to transmit data and computing reliability of the com-munication link. Another objective of this project is to define a solution for auto-converting path plan function, currently developed in Matlab, to run on Android devices. In addition, this solution should enable the developer to modify the path plan function in Matlab without having to consider Android programming. The differential method has been proposed for transferring secure data transfer using Bluetooth technol-ogy. This solution not only increases the security of the communication link but also improves transmission time. Another objective has been reached by developing a middleware function using C programming language and Android Native Development Kite (NDK) between An-droid and Matlab. The result of these solutions provides a reliable communication link and runs the developed path plan function in Android phone.