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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

The Amber of the Moment

Knippel, Mark Jacob, 1983- 06 1900 (has links)
1 score (vii, 56 p.) Includes one sound recording in AIFF (Audio Interchange File Format) / <italic>The Amber of the Moment</italic> is a thirteen-minute composition for orchestra. Inspiration for this piece is drawn from two sources: the novel <italic>Slaughterhouse Five</italic> by Kurt Vonnegut Jr., and my desire to utilize techniques derived from various musical cultures, including Balinese gamelan and African drumming and marimba playing. Although not directly narrative, much of the imagery portrayed in <italic>Slaughterhouse Five</italic> informed the emotional landscape of the piece. As to the use of techniques from other cultures, my aim is not to merely imitate them, but to utilize them in a manner appropriate to the tradition of orchestral concert music. / Committee in charge: Dr. David Crumb, Chairperson; Dr. Robert Kyr, Member; Dr. Stephen Rodgers, Member
2

Epidemiology of zoonoses in slaughterhouse workers in western Kenya

Cook, Elizabeth Anne Jessie January 2015 (has links)
Slaughterhouses are places where animals are slaughtered for food. In developing countries a lack of appropriate facilities and limited resources mean the slaughter industry is poorly regulated. Poor hygiene practices in slaughterhouses can result in the transmission of diseases from animals to people called zoonoses. Slaughterhouse workers are generally considered at increased risk of being exposed to such diseases due to their close contact with animals and animal products. The aims of this study were: to assess the current conditions in slaughterhouses and the knowledge, attitudes and practices of workers in ruminant and pig slaughterhouses in western Kenya; to determine the exposure of slaughterhouse workers to different zoonotic pathogens; to investigate the risk factors associated with exposure to these pathogens and to quantify the risk of zoonotic disease exposure for slaughterhouse workers compared to the general population. Slaughterhouses in western Kenya were visited between May 2011 and October 2012. Seven hundred and thirty-eight workers were recruited from 142 slaughterhouses. Overall, the slaughterhouses lacked facilities, with 65% (95% CI 63–67%) of slaughterhouses having a roof, cement floor and solid sides, 60% (95% CI 57–62%) had a toilet and 20% (95% CI 18–22%) hand-washing facilities. Less than half of workers 32% (95% CI 29–34%) wore personal protective clothing. Antemortem inspection was practiced at 7% (95% CI 6–8%) of slaughterhouses and 18% (95% CI 16–19%) of workers reported slaughtering sick animals. Slaughterhouse workers were screened for five zoonotic diseases. The unadjusted seroprevalence of the zoonotic diseases were: brucellosis 0.1% (95% CI 0.007– 0.8%); leptospirosis 13.4% (95% CI 11.1–16.1%); Q fever 4.5% (95% CI 3.2–6.2%); Rift Valley fever (RVF) 1.2% (95% CI 0.6–2.3%); taeniasis 1.8% (95% CI 1.0– 3.0%); and cysticercosis 2.6% (95% CI 1.7–4.0%). Risk factors for leptospirosis and Q fever were examined by multivariable logistic regression. Risk factors associated with leptospirosis seropositivity included: having wounds (OR 2.7; 95% CI 1.4–5.3); smoking at work (OR 1.8; 95% CI 1.1–3.0); eating at work (OR 2.1; 95% CI 1.2–3.6); and cleaning the intestines (OR 3.8; 95% CI 1.8–8.2). Protective factors were: working at a slaughterhouse where antemortem inspection was performed (OR 0.6; 95% CI 0.4–0.9). The risk factors significantly associated with Q fever seropositivity included: being intoxicated at work (OR 3.2; 95% CI 1.1–9.4). The odds ratio for leptospirosis seropositivity in slaughterhouse workers was determined to be 2.3 (95% CI 1.6–3.4) times that of the community. For Q fever the odds ratio for seropositivity in slaughterhouse workers was 1.9 (95% CI 1.0–3.8) times that of the community. This is the first report of a range of zoonotic pathogens in slaughterhouse workers in Kenya. This study indicated the potential risk factors for zoonotic disease exposure in slaughterhouses. The current working conditions in slaughterhouses in western Kenya are far below the recommended standard. Improvements need to be made to facilities and practices in all slaughterhouses. Training is recommended to improve awareness for workers, managers and inspectors of the risks of zoonotic disease exposure and methods to reduce it.
3

Avaliação sorológica e diagnóstico molecular para Leptospira spp. em bovinos abatidos em frigorífico do centro oeste paulista

Dutra, Marcelo Augusto Orsi January 2019 (has links)
Orientador: Simone Baldini Lucheis / Resumo: A leptospirose é uma zoonose de grande importância em saúde pública, considerada antropozoonótica com alta incidência e prevalência, negligenciada e de caráter ocupacional. Funcionários da linha de produção e manipuladores de sangue e vísceras em frigoríficos, podem infectar-se pelo contato direto com estes produtos, assim como os trabalhadores rurais nas propriedades, pelo contato e exposição direta a fômites e secreções destes animais. Esta enfermidade representa perdas econômicas importantes nos rebanhos, pois pode ocasionar abortos e fetos natimortos e, na sua forma subclínica, é comumente assintomática em bovinos. Amostras biológicas de sangue, fígado e rins de 144 bovinos abatidos em frigorífico localizado no centro-oeste paulista foram utilizadas para o diagnóstico da leptospirose com as técnicas de Soroaglutinação Microscópica (SAM) e Reação em Cadeia da Polimerase convencional (cPCR) para Leptospira spp. Os resultados sorológicos demostraram 71/144 (49,3%) animais reagentes à SAM para os seguintes sorovares: Djasiman (56,3%), Hardjoprajtino (40,8%), HardjoCTG (12,7%), Hardjo (11,3%), Hardjobovis (11,3%), Pyrogenes (9,8%), Wollfi (8,4%), Castellonis (7,0%), Pomona (4,2%), Grippotyphosa (1,4%), Hardjomini (1,4%), Icterohaemorrhagiae (1,4%) e Bratislava (1,4%). Os resultados moleculares evidenciaram a presença de DNA de Leptospira spp. em 25 animais (17,4%), sendo em rins de 21 animais, em fígado de cinco animais, em fígado e rins de dois animais e em sangue, de um (01)... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Leptospirosis is a zoonosis of great importance in public health, considered anthropozoonotic with high incidence and prevalence, neglected and of occupational character. Production line workers and handlers of blood and viscera in slaughterhouses can be infected by direct contact with these products, as well as rural workers on the farms, by contact and direct exposure to fomites and secretions of these animals. This disease represents important economic losses in the herds, since it can cause abortions and stillborn fetuses and, in its subclinical form, is commonly asymptomatic in cattle. Biological blood, liver and kidneys samples from 144 cattle slaughtered in a slaughterhouse located in central-western São Paulo were used for the diagnosis of leptospirosis using the Microscopic Agglutination Test (MAT) and Conventional Polymerase Chain Reaction (cPCR) techniques for Leptospira spp. The serological results showed 71/144 (49.3%) animals reactive to MAT for the following serovars: Djasiman (56.3%), Hardjoprajtin (40.8%), HardjoCTG (12.7%), Hardjo 3%), Hardjobovis (11.3%), Pyrogenes (9.8%), Wollfi (8.4%), Castellonis (7.0%), Pomona (4.2%), Grippotyphosa, Hardjomini (1.4%), Ichterohaemorrhagiae (1.4%) and Bratislava (1.4%). Molecular results evidenced the presence of Leptospira spp. in 25 animals (17.4%): the kidneys of 21 animals, in the liver of five animals, in the liver and kidneys of two animals and in blood of one (01) animal. These results indicate an alert to the sani... (Complete abstract click electronic access below) / Mestre
4

Isolamento e caracterização de Campylobacter spp. em amostras de fezes e carcaças de suínos provenientes de abatedouros do Estado de São Paulo / Isolation and characterization of Campylobacter spp. in samples of swine feces and carcasses collected in São Paulo State slaughterhouses

Campos, Fabíola Ribeiro 15 February 2007 (has links)
A importância da espécie suína na transmissão de Campylobacter spp. assemelha-se aos demais grupos de animais que se destinam à produção de carne, incluindo aves, bovinos e ovinos. Os objetivos deste estudo foram isolar Campylobacter spp. a partir de fezes e carcaças de suínos abatidos no Estado de São Paulo; identificar as espécies de Campylobacter spp. presentes nos animais abatidos; caracterizar os isolados obtidos através do Polimorfismo do Comprimento de Fragmentos Amplificados (AFLP). Para tal, foram utilizadas 120 amostras de fezes e 120 suabes de carcaças de suínos, colhidas de quatro diferentes abatedouros do Estado de São Paulo. Das 120 amostras de fezes analisadas, 30 foram positivas para o isolamento de Campylobacter coli (25%) e duas foram positivas para isolamento de Campylobacter jejuni (1,6%). Todas as amostras analisadas de suabes de carcaça foram negativas para Campylobacter spp. As estirpes isoladas que apresentaram características bioquímicas sugestivas de Campylobacter spp. foram submetidas ao teste de susceptibilidade ao ácido nalidixico e cefalotina, destas 19,16% (23/120) apresentaram resistência ao ácido nalidixico apesar de todas as características bioquímicas indicarem se tratar de Campylobacter coli. Foram selecionadas para a análise genotípica 38 amostras isoladas, sendo 36 de C. coli e dois de C. jejuni. A análise dos isolados através do AFLP revelou a presença de 28 perfis que foram designados P1 a P28. A técnica discriminou as cepas de acordo com a espécie, porém, uma cepa previamente caracterizada como C. coli foi agrupada com isolados de C. jejuni. Não foi possível estabelecer a correlação entre os isolados e o abatedouro de origem, no entanto observa-se uma forte tendência dos isolados resistentes ao ácido nalidixico em formar grupamentos de maior similaridade. / The importance of swine species in the transmission of Campylobacter spp. resembles to the other groups of animals that are destined to the meat production, including bovine and ovine animals. The objectives of this study were to isolate Campylobacter spp. from swine feces and carcasses slaughtered in São Paulo State; to identify the Campylobacter spp. species presents in the slaughtered animals; to characterize the isolated samples by amplified fragment length polymorphism (AFLP), for this, 120 swine feces samples and the same number of carcasses swabs were collected of four different slaughterhouses in São Paulo State. From 120 feces samples, 30 (25%) were positive for Campylobacter coli isolation and two (1,6%) were positive for Campylobacter jejuni. All the analyzed carcass swabs samples were negatives for Campylobacter spp. The isolated samples that presented suggestive biochemical characteristics of Campylobacter spp. were submitted to the susceptibility test to the nalidixic acid and cefalotine, from that 19.16% (23/120) presented resistance to the nalidixic acid in spite of all the biochemical characteristics indicate that they were Campylobacter coli. They were selected for the genotypic analysis 38 isolated samples, being 36 of C. coli and two of C. jejuni. The analysis of the 38 tested samples by AFLP showed the presence of 28 profiles that had been assigned P1 to P28. The technique discriminated the samples in agreement with the species, however, one sample previously characterized as C. coli was clustered as C. jejuni. It was not possible to establish the correlation between the isolated samples and the origin slaughterhouse, however a strong tendency of the isolated samples acid nalidixic resistant to create clusters with more similarity.
5

Mechanism of zeolite activity in biogas co-digestion

Hansson, Anna January 2011 (has links)
Biogas is a source of renewable energy and is produced at anaerobic conditions. The gas consists mainly of methane (55-70 %) and carbon dioxide (30-45 %). Biogas can be used as vehicle fuel after the gas has been upgraded to a methane content of approximately 97 %. There are several companies in Sweden producing biogas. Svensk biogas AB in Linköping is one of the largest. The company has two biogas production plants; one in Linköping and one in Norrköping. To meet the surge demand for biogas it is not only important to increase the volumetric capacity of the digesters, but also to optimize the process at the existing production plants in different ways. Zeolites, a clay mineral, have earlier been shown to have a positive effect on anaerobic digestion of certain substrates. The aim of this master’s thesis was to investigate if the organic loading rate could be increased and/or if the hydraulic retention time could be reduced by addition of zeolites to a reactor treating slaughterhouse waste as a substrate. The aim was further to investigate which substance/substances that zeolites possibly could affect. Addition of the zeolite clinoptilolite in a continuously stirred lab tank reactor showed a significantly lower accumulation of volatile fatty acids compared to that in a control reactor without zeolites added, when the hydraulic retention time was kept low (30 days) and the organic loading rate was high (4.8 kg VS/ (m3 × day)). The same results were observed upon zeolite addition in a batch experiment, which also showed a decreased lag phase. Neither the specific gas production nor the methane concentration was significantly affected by addition of zeolites. Furthermore, addition of a possible inhibitor, long-chain fatty acids (LCFA), increased the lag phase further when slaughterhouse waste was used as a substrate. The conclusion from the observed results is that a metabolite or metabolites produced during the anaerobic degradation is/are the reason to inhibition and an increased lag phase.
6

Análise das condições de trabalho em uma empresa do setor frigorífico a partir de um enfoque macroergonômico

Delwing, Eduardo Becker January 2007 (has links)
Centrada em uma análise macroergonômica, esta pesquisa teve como objetivo identificar os problemas mais importantes e propor soluções de melhoria nas condições de trabalho no setor de cortes de um frigorífico de aves, focando principalmente a redução dos Distúrbios Osteomusculares Relacionados ao Trabalho. O diagnóstico das atividades desenvolvidas na unidade teve como base a observação direta e os Itens de Demanda Ergonômica (IDE’s) identificados por uma amostra de trabalhadores. Os principais itens apontados envolvem aspectos do posto de trabalho como o acesso a botas térmicas, organização do rodízio de cadeiras, afiação de facas, quantidades de cadeiras, distância para chegar ao refeitório, além de se ter que caminhar por um caminho sem cobertura contra intempéries. Em seguida, são citados problemas ambientais tais como: o frio no local de trabalho (que é climatizado com temperatura entre 10 e 12°C, atendendo à legislação do Ministério da Agricultura) e o ruído elevado (até 92 dB(A)), originado pelos exaustores e ventiladores do sistema de refrigeração que foi destacado como causa de dores de cabeça. As questões relativas à organização do trabalho dizem respeito ao ritmo de produção da nórea transportadora, principalmente no final da jornada, quando os trabalhadores estão mais cansados, e ao acúmulo de produtos na esteira. Também foi salientada a pressão para maior produção por parte das chefias, falta de preparo das chefias, falta de organização do rodízio de funções e a cobrança de produção excessiva para os novatos que necessitam de um melhor treinamento. A dor nos braços, pernas e coluna, que pode estar associada ao trabalho repetitivo em posição estática, é o registro que mais aparece nos relatórios médicos, e foram apontadas no diagrama de desconforto/dor adaptado de Corlett e Bishop (1976). / Based on a macroergonomic analysis, this study identifyed the most significant problems of the work carried out in a cutting sector of a poultry slaughterhouse and proposed solutions for reducing Work Related Osteomuscular Disorders. Ergonomic diagnosis was based on direct observation as well as on the identification of the Ergonomic Demand Items by a sample of workers. The main items highlighted involve aspects regarding the workstation, such as the access to thermal boots, access to chair (for alternating standing and sitting postures during work), quality of knife sharpening, distance to reach the cafeteria, and also the need to walk on an uncovered path (therefore being exposed to bad weather). Environmental problems were also pointed out, such as the cold in the working site (where the temperature varies between 10 to 12ºC, in compliance with Agriculture Department legislation), and the loud noise (reaching 92 dB(A)), originated from exhausters and fans of the cooling system which is considered the cause of headache. Work organization problems are mainly related to the conveyor belt’s production speed (especially at the end of the shift, when workers are more tired), and to the accumulation of poultry on the belt. It was also mentioned the pressure for an increase in production (this is especially true in the case of new workers who require a better training), and the lack of managers ability for dealing with people and organizing the chair rotation scheme for work posture alternation. Sore arms, legs and spine, possibly associated to repetitive work in static position, are the most common complaints in medical records, and were shown on Corlet and Bishop’s adapted diagram of discomfort/pain (1976).
7

Avaliação qualitativa da água residuária de abatedouro de aves para fins de reúso em irrigação

Minghini, Israel [UNESP] 20 November 2007 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:26:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-11-20Bitstream added on 2014-06-13T19:14:05Z : No. of bitstreams: 1 minghini_i_me_botfca.pdf: 448467 bytes, checksum: b2a4f494cb357e662adbf52c2039ccbb (MD5) / O objetivo do presente trabalho consistiu na avaliação de parâmetros de qualidade da água residuária proveniente de abatedouro de aves, visando analisar a possibilidade de reutilizá-la na irrigação de culturas agrícolas, após sua caracterização. As águas residuárias do abatedouro de aves, localizado na cidade de Pereiras – SP, foram coletadas em diferentes dias e épocas do ano e analisadas quanto aos seguintes parâmetros: pH, temperatura, condutividade elétrica, fósforo total, nitrato, nitrito, sólidos sedimentáveis, ferro, alumínio, turbidez, Salmonellas sp, macro e micronutrientes. As temperaturas do ar e da água foram medidas no momento da coleta.A discussão dos resultados obtidos esteve de acordo com a Resolução do Conselho Nacional do Meio Ambiente (CONAMA) nº 357, de 17 de março de 2005, que estabelece os padrões para classificação dos corpos de água. A condutividade elétrica foi avaliada segundo padrões propostos pela Companhia de Tecnologia de Saneamento Básico (CETESB, 2005) e o alumínio, segundo valor estabelecido pelo Ministério da Saúde – Portaria nº 1 469. Os resultados obtidos permitem concluir que a água residuária analisada, não deve ser descartada diretamente em corpos hídricos naturais, porém, pode ser utilizada na irrigação de culturas agrícolas, após simples desinfecção. / The aim of this search consisted in comparing the quality of wastewater from a poultry slaughterhouse, trying to analyze the possibility of using it in the irrigation of agricultural growing, after its make-up. Samples were colleted from wastewater from a poultry slaughterhouse in Pereiras – SP, in different days or time of the year, analyzing the following: pH, temperature, electrical conductivity, total phosphorus, nitrate, nitrite, sedimentary solids, iron, aluminium, muddy, salmonella, macro and micro nutrients. The temperature of the air and of the water were measured at collection time.The results were evaluated according to the Resolution of the Environment National Council (CONAMA) nº 357, on March 17th- 2005, which establish the patterns to classify the water contents. The electrical conductivity was evaluated according to the amount suggested by CETESB/2005 and the aluminium according to the established amount by Health Ministry – Regulation nº 1.469. The obtained results allow to conclude that the analyzed wastewater, must not be rejected in natural water contents, however, it can be used in the irrigation of agricultural growing, after simple disinfection.
8

Análise das condições de trabalho em uma empresa do setor frigorífico a partir de um enfoque macroergonômico

Delwing, Eduardo Becker January 2007 (has links)
Centrada em uma análise macroergonômica, esta pesquisa teve como objetivo identificar os problemas mais importantes e propor soluções de melhoria nas condições de trabalho no setor de cortes de um frigorífico de aves, focando principalmente a redução dos Distúrbios Osteomusculares Relacionados ao Trabalho. O diagnóstico das atividades desenvolvidas na unidade teve como base a observação direta e os Itens de Demanda Ergonômica (IDE’s) identificados por uma amostra de trabalhadores. Os principais itens apontados envolvem aspectos do posto de trabalho como o acesso a botas térmicas, organização do rodízio de cadeiras, afiação de facas, quantidades de cadeiras, distância para chegar ao refeitório, além de se ter que caminhar por um caminho sem cobertura contra intempéries. Em seguida, são citados problemas ambientais tais como: o frio no local de trabalho (que é climatizado com temperatura entre 10 e 12°C, atendendo à legislação do Ministério da Agricultura) e o ruído elevado (até 92 dB(A)), originado pelos exaustores e ventiladores do sistema de refrigeração que foi destacado como causa de dores de cabeça. As questões relativas à organização do trabalho dizem respeito ao ritmo de produção da nórea transportadora, principalmente no final da jornada, quando os trabalhadores estão mais cansados, e ao acúmulo de produtos na esteira. Também foi salientada a pressão para maior produção por parte das chefias, falta de preparo das chefias, falta de organização do rodízio de funções e a cobrança de produção excessiva para os novatos que necessitam de um melhor treinamento. A dor nos braços, pernas e coluna, que pode estar associada ao trabalho repetitivo em posição estática, é o registro que mais aparece nos relatórios médicos, e foram apontadas no diagrama de desconforto/dor adaptado de Corlett e Bishop (1976). / Based on a macroergonomic analysis, this study identifyed the most significant problems of the work carried out in a cutting sector of a poultry slaughterhouse and proposed solutions for reducing Work Related Osteomuscular Disorders. Ergonomic diagnosis was based on direct observation as well as on the identification of the Ergonomic Demand Items by a sample of workers. The main items highlighted involve aspects regarding the workstation, such as the access to thermal boots, access to chair (for alternating standing and sitting postures during work), quality of knife sharpening, distance to reach the cafeteria, and also the need to walk on an uncovered path (therefore being exposed to bad weather). Environmental problems were also pointed out, such as the cold in the working site (where the temperature varies between 10 to 12ºC, in compliance with Agriculture Department legislation), and the loud noise (reaching 92 dB(A)), originated from exhausters and fans of the cooling system which is considered the cause of headache. Work organization problems are mainly related to the conveyor belt’s production speed (especially at the end of the shift, when workers are more tired), and to the accumulation of poultry on the belt. It was also mentioned the pressure for an increase in production (this is especially true in the case of new workers who require a better training), and the lack of managers ability for dealing with people and organizing the chair rotation scheme for work posture alternation. Sore arms, legs and spine, possibly associated to repetitive work in static position, are the most common complaints in medical records, and were shown on Corlet and Bishop’s adapted diagram of discomfort/pain (1976).
9

Manejo no pré-abate de bovinos: aspectos comportamentais e perdas econômicas por contusões

Almeida, Leonel Augusto Martins [UNESP] 08 1900 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:27:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-08Bitstream added on 2014-06-13T19:14:31Z : No. of bitstreams: 1 almeida_lam_me_jabo.pdf: 495255 bytes, checksum: d6d7d873f96a2cd34d98301e1c31627a (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O presente trabalho teve como objetivo, observar o panorama das condições de pré-abate de bovinos, as práticas com relação ao bem-estar animal e também fazer um levantamento da percentagem (freqüência de ocorrência) de contusões em carcaça e suas possíveis conseqüências. Para isso, durante o ano de 2004, foram monitorados cinco frigoríficos, sendo aplicados questionários e formulários de monitorações, para conhecer a estrutura e aplicação de procedimentos de pré-abate aos animais. Observou-se que apenas 86,00% dos animais abatidos recebiam atordoamento adequado, com eficiência da insensibilização de 96,00%. Ainda durante o manejo préabate, as quedas dos animais chegaram a 22,00% e as vocalizações ocorreram em até 30,00%. Houve uma média de 70,00% de contusões nos animais monitorados, com um peso médio de 147,11 g retirados de cada animal devido a lesões nas carcaças, o que representou 34.953,34 kg de perdas de carne ao ano. Esses aspectos indicam a importância e a necessidade de reformulação dos conceitos da cadeia produtiva para manter a condição (habilitação) do país como exportador de carne. / The present research had as aim: to observe how were the pre-slaughter conditions of bovines, the practices for animal welfare and also the incidence of bruises in carcass and its possible consequences. For that, during the year of 2004, five slaughterhouses were monitored, being applied questionnaires and monitoring forms to know the structure and application of pre-slaughter procedures to the animals. It was observed that 86,00% of the slaughtered animals just received appropriate stunning, causing efficiency of the insensible animals in 96,00%. Still during the pre-slaughter handling, the falls of the animals were 22,00% and the vocalizations happened in up to 30,00%. There was an average of 70,00% of bruises in the monitored animals, representing an average of 147,11 g retired of each animal due the lesions in the carcasses, that represented 34.953,34 kg of meat losses a year. Those aspects indicate the importance and need of reformulation of productive chain concepts to maintain the meat exporter condition.
10

Detecção de Escherichia coli shigatoxigênica (STEC) e enteropatogênica (EPEC) em carcaças e fezes de suínos abatidos na região de Ribeirão Preto – SP e perfil de susceptibilidade dos isolados frente a diferentes antimicrobianos

Borges, Clarissa Araújo [UNESP] 24 February 2011 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-24Bitstream added on 2014-06-13T18:31:28Z : No. of bitstreams: 1 borges_ca_me_jabo.pdf: 259288 bytes, checksum: aee47e190d42e2e6677cc13511880fb0 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Escherichia coli destaca-se entre os principais patógenos de importância na suinocultura e saúde pública e apresenta diversos patótipos, caracterizados pela presença de diferentes fatores de virulência. Os objetivos deste trabalho foram detectar genes de virulência e determinar a sua freqüência, bem como caracterizar o perfil de resistência às drogas antimicrobianas de estirpes de E. coli shigatoxigênica (STEC) e enteropatogênica (EPEC) isoladas de fezes e carcaças de suínos abatidos na região de Ribeirão Preto - SP. Esse estudo foi conduzido com 226 amostras de fezes e 215 amostras de carcaça de suínos colhidas de três diferentes abatedouros. As estirpes isoladas foram submetidas ao teste bioquímico para confirmação da espécie, teste de suscetibilidade a antimicrobianos, teste para determinação dos sorogrupos e PCR para detecção dos genes stx1, stx2, eae, ehxA, efa1, saa, lpfAO113, lpfAO157/OI-141, lpfAO157/OI-154, toxB, iha e bfp, foram isolados 3,4% de EPEC e 0,2% de STEC. Os fatores de virulência foram encontrados em quatro diferentes combinações: eae + lpfAO113 (1,6%); eae (1,4%); eae + toxB + lpfAO157/OI-141 + ehxA (0,4%) e stx2 (0,2%). Não houve isolados positivos para os genes lpfAO157/OI-154, saa, efa1, iha e bfp. Todos os isolados foram sensíveis à nitrofurantoína e 93,75% resistentes à tetraciclina. Os resultados mostraram que amostras provenientes de suínos da região de Ribeirão Preto-SP apresentaram E. coli que contêm genes de virulência associados a STEC e EPEC. Esse fato é um alerta para que esses animais sejam monitorados, pois podem ser potenciais reservatórios de STEC e EPEC causadoras de doenças em humanos / Escherichia coli stands out among the main pathogens of importance to the swine industry and public health and presents several pathotypes characterized by the presence of different virulence factors. The aims of this study were to determine the frequency, detect virulence genes and characterize the resistance profile to antimicrobial drugs of Shiga toxigenic Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains isolated from feces and carcasses of pigs slaughtered in the region of Ribeirão Preto – SP. This research was carried out with 226 fecal samples and 215 carcasses samples from swine collected from three different slaughterhouses. The strains isolated were submitted to antimicrobial susceptibility test, biochemical test to confirm the specie, test to determine the serogroups and PCR for detection of genes stx1, stx2, eae, ehxA, efa1, saa, lpfAO113, lpfAO157/OI-141, lpfAO157/OI-154, toxB, iha and bfp, 0,2% of STEC and 3,4% of EPEC were isolated. The virulence factors were found in four different combinations: eae + lpfAO113 (1,6%); eae (1,4%); eae + toxB + lpfAO157/OI-141 + ehxA (0,4%) and stx2 (0,2%). There were no positive isolate for genes lpfAO157/OI-154, saa, efa1, iha and bfp. All isolates were susceptible to nitrofurantoin and 93.7% resistant to tetracycline. The results showed that samples from swines in the region of Ribeirão Preto have E. coli containing virulence genes associated with EPEC and STEC strains. This fact is a warning that these animals should be monitored because they can be potencial reservoirs of STEC and EPEC that cause disease in human

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