Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Fusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA. / Fusarium spp. é o agente causal de podridões radiculares em diversas culturas. No sistema plantio direto, áreas compactadas favorecem a incidência dessas doenças. Esses fungos podem também infectar os grãos e produzir micotoxinas. Trichoderma spp. vem apresentando resultados promissores e pode ser utilizado no manejo integrado de doenças provocadas por patógenos de solo. Os objetivos deste trabalho foram: quantificar e correlacionar populações de Fusarium spp., Trichoderma spp. e outros (fungos e bactérias) em um solo com características físicas indicativas de compactação, nos cultivos de aveia preta e soja; identificar morfológica e molecularmente os isolados de Fusarium spp.; caracterizar geneticamente isolados de Trichoderma spp.; testar a eficiência de controle in vitro e in vivo de Trichoderma spp. versus Fusarium spp.; e quantificar a produção de deoxinivalenol (DON) e zearalenona (ZEA) pelos isolados de Fusarium spp. Amostras de solo foram coletadas no município de Victor Graeff (RS) em uma área previamente mapeada quanto à compactação do solo. As coletas foram feitas em três profundidades (0-5; 5-10 e 10-15 cm) e em seis níveis de compactação estabelecidos pelas medidas de resistência à penetração (Rp) (pontos com maior Rp: 3,4; 4,6 e 5,0 MPa; e menor Rp: 0,3; 1,3 e 2,2 MPa). Nas amostras coletadas foram realizadas avaliações de população fúngica e microbiana (diluições seriais) e de características físicas do solo. Fungos dos gêneros Fusarium e Trichoderma, quando presentes nas diluições seriais, foram isolados para posterior identificação morfológica e molecular (baseada nas regiões TEF-1α e ITS, respectivamente). Foram realizados testes in vitro (confrontação direta) e in vivo (em aveia preta e soja) para avaliar a eficiência de controle de Trichoderma spp. versus Fusarium spp. A produção de DON e ZEA foi verificada pelo método Elisa e colunas de imunoafinidade, respectivamente. Em aveia preta cultivada após a soja, as características físicas e populacionais do solo se correlacionaram mais pronunciadamente, sendo que maiores populações de Fusarium spp. e Trichoderma spp. foram encontradas nas profundidades 5-10 e 10-15 cm, respectivamente. Identificaram-se 13 espécies de Fusarium e a região TEF-1α foi eficiente para sua distinção. T. koningiopsis, T. tomentosum e T. asperellum foram identificados, totalizando cinco isolados e todos eles apresentaram bom potencial de controle de Fusarium spp. em soja. Em aveia preta, destacaram-se como promotores de crescimento radicular, incrementando o peso fresco de plântulas. Em soja, isolados de F. oxysporum e F. proliferatum foram patogênicos e provocaram tombamento de plântulas. Para aveia, os isolados de F. graminearum não proporcionaram a observação desse sintoma. F. graminearum e F. solani produziram tanto DON quanto ZEA, enquanto que F. proliferatum e F. oxysporum produziram apenas ZEA.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第21097号 / 工博第4461号 / 新制||工||1693(附属図書館) / 京都大学大学院工学研究科都市環境工学専攻 / (主査)教授 伊藤 禎彦, 教授 田中 宏明, 准教授 西村 文武 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

Made available in DSpace on 2016-08-31T13:24:16Z (GMT). No. of bitstreams: 1 FrancicleitonFC_DISSERT.pdf: 2264503 bytes, checksum: 32bac5ee4fa11da99750236b5690bdc7 (MD5) Previous issue date: 2013-07-26 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / The utilization of treated domestic wastewater for irrigation is an alternative to control pollution of surface and groundwater, as well as a way to provide water and nutrients for plants. Thus, the present work was to study the changes in soil characteristics and performance of irrigation (Bubbler) operating system with wastewater from domestic sources. To this end, was mounted an experimental area of 576 m2 in the Miracles settlement in Apodi-RN, endowed with a system for primary treatment of domestic sewage, and four subunits of the irrigation system "Bubbler". The treatments were made up of different proportions of primary domestic sewage (PDS) and water supply (WS) were: T1 (0% of PDS and 100% of WS, more chemical fertilizer [witness]), T2 (33 % of PDS and 67% of WS), T3 (67% of PDS and 33% of WS) and T4 (100% of PDS and 0% of WS). To analyze the performance of the irrigation system, it was used a split plot arrangement having the plots and subplots evaluation times (0, 120 and 240 days of operation). In the analysis of soil physic-chemical characteristics was used split split plot scheme with the treatments on plots, the sampling depths on subplots (0 to 0.20 m and 0.20 to 0.40 m) and the evaluation periods on subsubplots (0, 120 and 240 days after transplanting). The experimental design used was the completely randomized blocks with four treatments and six repetitions. The Data were submitted to analyzes of variance and regression and tests in the average. During the experimental period was determined physico-chemical and microbiological characteristics of the PDS and WS, and the results indicated that the quality of PDS and WS do not compromise the use for the agricultural crop, and may be used to management criteria. The highest levels of emitter clogging were found in T4 and T3. The T2 provides better performance of the irrigation system, showing better uniformity coefficient (CUC and CUD) and application efficiency (AE). With respect to soil characteristics, it was observed that the values of EC, P, K, Ca and Al there were no statistically significant changes in treatments, altering with only soil depth and the time of treatment application. The characteristics pH, V, SB and Zn tended to reduce with increasing proportion of PDS and the time of application. The characteristics N, MO, Mg, Na, PST, H + Al, m, t, CTC, Cu, Fe and Mn tended to be greater with increasing proportion of PDS and the time of application. The total coliforms and fecal coliform tended to be greater with increasing proportion of PDS and reduced with increasing soil depth / A utilização de esgoto doméstico tratado na irrigação é uma alternativa para controle da poluição das águas superficiais e subterrâneas, além de uma maneira de disponibilizar água e nutrientes para as plantas. Diante disso, o presente trabalho teve o objetivo de estudar as alterações nas características do solo e no desempenho do sistema de irrigação (Bubbler) operando com água residuária de origem doméstica. Para tal, foi montada uma área experimental de 576 m2 no assentamento Milagres em Apodi-RN, dotada de um sistema para tratamento primário do esgoto doméstico, e quatro subunidades do sistema de irrigação Bubbler . Os tratamentos utilizados foram compostos de diferentes proporções do esgoto doméstico primário (EDP) e de água de abastecimento (AA), sendo: T1(0% de EDP e 100% de esgoto AA, mais adubação química [testemunha]); T2(33% de EDP e 67% de AA); T3(67% de EDP e 33% de AA); e T4(100% de EDP e 0% de AA). Para análise do desempenho do sistema de irrigação, utilizou-se um esquema de parcelas subdivididas tendo nas parcelas os tratamentos e nas subparcelas os tempos de avaliação (0, 120 e 240 dias de operação). Na análise das características físico-químicas do solo foi utilizado o esquema de parcelas subsubdivididas tendo nas parcelas os tratamentos, nas subparcelas as profundidades de amostragem (0 a 0,20 m e 0,20 a 0,40m) e nas subsubparcelas os períodos de avaliação (0, 120 e 240 dias após o transplantio). O delineamento experimental utilizado foi o de blocos inteiramente casualizado com quatro tratamentos e seis repetições. Os dados foram submetidos à análise de variância e de regressão e testes de média. Durante o período experimental determinou-se as características físico-químicas e microbiológicas do EDP e da AA, e os resultados indicaram que a qualidade do EDP e da AA não comprometem o uso para o cultivo agrícola, podendo ser utilizado com critérios de manejo. Os maiores níveis de entupimento de emissores foram encontrados nos tratamentos T4 e T3. O T2 proporcionou melhor desempenho do sistema de irrigação, apresentando melhores coeficientes de uniformidades (CUD e CUC) e eficiência de aplicação (EA). Com relação às características do solo, observou-se que os valores da CE, P, K, Ca e Al não sofreram alterações estatisticamente significativas dos tratamentos, alterando-se somente com a profundidade do solo e com o tempo de aplicação dos tratamentos. As características pH, V, SB e Zn apresentaram tendência de redução com o aumento da proporção de EDP e com o tempo de aplicação. As características N, MO, Mg, Na, PST, H+Al, m, t, CTC, Cu, Fe e Mn apresentaram tendência de aumento com o aumento da proporção de EDP e com o tempo de aplicação. Os coliformes totais e os coliformes termotolerantes apresentaram tendência de aumento com o aumento da proporção de EDP e redução com o aumento da profundidade do solo

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / Restos de culturas e outros materiais vegetais tÃm sido usados como cobertura morta no sistema de plantio direto em vÃrias regiÃes do Brasil. No Nordeste essa prÃtica à pouco comum e existe desconhecimento sobre o comportamento dessas coberturas nas condiÃÃes do semiÃrido quanto à degradaÃÃo e seus efeitos sobre a produtividade das culturas e caracterÃsticas fÃsico-quÃmicas do solo. Uma pesquisa foi conduzida na Fazenda Lavoura Seca, QuixadÃ, CearÃ, com o objetivo de avaliar a produtividade de grÃos de milho (Zea mays L) e feijÃo-caupi (Vigna unguiculata L Walp.) e algumas caracterÃsticas de um Argissolo Vermelho Amarelo quando submetido a um sistema de plantio direto em cinco tipos de cobertura morta provenientes de: vegetaÃÃo natural; milheto (Pennisetum americanum sin. tiphoydes); braquiÃria (Brachiaria brizantha); sorgo forrageiro (Sorghum bicolor L. Moench); e do plantio convencional, em dois sistemas de rotaÃÃo de culturas SPD1 (feijÃo-caupi em sucessÃo ao milho) e SPD2 (milho em sucessÃo ao feijÃo-caupi). As coberturas mortas foram avaliadas quanto à duraÃÃo, taxa de decomposiÃÃo e seus efeitos sobre as caracterÃsticas do solo densidade, densidade de partÃculas, porosidade total, micro porosidade, macro porosidade e teor de umidade, na profundidade de zero a 20 cm, nos anos agrÃcolas 2008 e 2009. NÃo foram constatadas diferenÃas significativas entre a biomassa produzida pela vegetaÃÃo natural e a proporcionada pela braquiÃria, milheto e sorgo forrageiro no SPD1 e SPD2. A degradaÃÃo dos resÃduos no perÃodo de avaliaÃÃo foi maior onde o feijÃo-caupi foi sucedido pelo milho. A produtividade do milho nÃo diferiu entre as coberturas e plantio convencional em 2008 e 2009. A produtividade de grÃos do feijÃo-caupi foi maior no plantio convencional do que nos tratamentos com cobertura morta podendo ser atribuÃdo ao melhor controle das plantas daninhas no sistema convencional. As diferentes coberturas mortas proporcionaram aumento na densidade do solo e diminuiÃÃo na porosidade total e macro porosidade / Crops and others vegetal residues are used as death cover in minimum tillage in several regions of Brazil. In the Northeast this practice is not yet generalized and there is a poor knowledge about the plant residues degradation under semi-arid conditions and its effects on crop productivity and the physical and chemical soil characteristics. This work was carried out at Dry Farm Experimental Station, Quixada, Ceara, in order to evaluate the corn and cowpea yield growing under minimum tillage with two crop rotation systems, SPD1 (cowpea following corn) and SPD2 (corn following cowpea) and five types of death cover originated from natural vegetation, millet (Pennisetum americanum sin. tiphoydes); brachiaria grass (Brachiaria brizantha); forage sorghum, (Sorghum bicolor L. Moench) and conventional tillage. It was also evaluated the effects of these rotation systems and deaths covers over soil density, particle density, micro and macro porosity, percentage of soil humidity at 20cm depth of a Red Yellow Argissol during 2008 and 2009. It was not found significant differences between the biomass yields of brachiaria grass, millet and sorghum in SPD1 and SPD2. The deaths cover degradation during the two years of evaluation was higher where the cowpea was followed by corn. The corn yield was not significantly different between the death covers and conventional tillage. The better cowpea yield under conventional tillage could be caused by a better weed control. The death cover tested increased the soil density and decreases the macro and total soil porosity

This thesis is a contribution to the work of the Texas Environmental Observatory (TEO), which provides environmental information from the Greenbelt Corridor (GBC) of the Elm Fork of the Trinity River. The motivation of this research is to analyze the short-term water dynamic of soil in response to the substantial rainfall events that occurred in North Texas in 2007. Data collected during that year by a TEO soil and weather station located at the GBC includes precipitation, and soil moisture levels at various depths. In addition to these field measurements there is soil texture data obtained from lab experiments. By comparing existing water dynamic models, water balance equations were selected for the study as they reflect the water movement of the soil without complicated interrelation between parameters. Estimations of water flow between soil layers, infiltration rate, runoff, evapotranspiration, water potential, hydraulic conductivity, and field capacity are all obtained by direct and indirect methods. The response of the soil at field scale to rainfall event is interpreted in form of flow and change of soil moisture at each layer. Additionally, the analysis demonstrates that the accuracy of soil characteristic measurement is the main factor that effect physical description. Suggestions for model improvement are proposed. With the implementation of similar measurements over a watershed area, this study would help the understanding of basin-scale rainfall-runoff modeling.

The thesis deal with the soil sealing and the rehanding with the uncovering of soil cultural layers. This problem is describe on the example of the industrial zone Kolín - Ovčáry. In the thesis there is the evaluation of the soil characteristics and their changes in areas where due to the trensfer of the cultural layer was created completely new structure of soil cover compact of soil units with artificial stratigraphy of profile. For this area the new classification subsumtion is suggested. The overall impacat of the soilsealig and implementation of the uncovering of soil cultural layers, despite of local incerase of soil fertility parameters, we evaluate as the degradation of soil cover.

No description available.

En région méditerranéen, les incendies de forêt sont un problème majeur vis-à-vis de la désertification du milieu. L'utilisation de composts est considérée comme une solution pour la régénération du sol et de la végétation.Tout d'abord, en laboratoire, les effets de trois types de compost et de leur mode d'application ont été testés : un compost de déchets verts (DV), un compost de boues de station d'épuration (B) et un compost d'ordures ménagères résiduelles (OMR). Les différents paramètres du sol ont été améliorés de façon plus importante par le compost OMR (le moins mature) alors que le compost DV (le plus mature) n'a que peu d'effet par rapport au contrôle. De plus, lorsque le compost est enfoui dans le sol, la réduction du ruissellement est faible alors qu'appliqué en surface, elle est importante et favorise la percolation et la rétention principalement avec le compost OMR. Lors d'une expérimentation in situ, l'évolution du sol et de la végétation a été étudiée après un apport de compost de boues de station d'épuration. Lors des premiers mois, une hausse de la biomasse des espèces herbacées a été observée. Bien qu'aucun effet n'ait été observé pour les autres espèces, la phytomasse était plus hétérogène sur les parcelles amendées alors que la biodiversité y était plus homogène. Un transfert rapide et durable d'éléments nutritifs du compost vers le sol a été observé ainsi qu'une hausse de la teneur en eau du sol des parcelles amendées. Les teneurs en nutriments des plantes augmentent aussi mais varient selon l'espèce et l'élément considérés. De plus, aucun effet négatif n'a été observé quant aux teneurs en éléments traces métalliques du sol et des végétaux. / In Mediterranean region, forest fires are a major problem leading to the desertification of the environment. Use of composts is considered as a solution for soil and vegetation rehabilitation.First, we observed the effects of three urban composts and their mode of application (laid on the soil surface or mixed into the soil) on soil restoration after fire: a municipal waste compost (MWC), a compost of sewage sludge (SSC) and a green waste compost (GWC) under laboratory conditions. Input of composts increased organic matter and soil nutrient content, and enhanced carbon and nitrogen mineralisation and total microbial biomass throughout the incubations. MWC induced the highest improvement while GWC input had no significant effect compared to the control. Composts mixed with soil weakly limited runoff whereas composts laid at the soil surface significantly reduced runoff and increased percolation and retention, particularly with the MWC.Then, we studied in situ the effects of a SSC on soil and vegetation regeneration after fire. The first months after amendment, this input increased grass species biomass. Although no significant effect was observed on other studied plants, plant biomass was more heterogeneous on amended plots while plant biodiversity was more homogeneous. The use of compost increased rapidly soil nutrient content and soil water content but plant nutrition was improved more or less durably depending species. Compost effects on microbial activities were low and only sporadic. Moreover, no negative effect was observed during the two years of experimentation.

Made available in DSpace on 2017-07-10T17:37:33Z (GMT). No. of bitstreams: 1 Luciana_Cleci_de_Oliveira.pdf: 753219 bytes, checksum: cc1a5575b73ca9b97e749c6fa03d3cd3 (MD5) Previous issue date: 2009-06-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Due the lack of informatios about fertilization on lettuce (Lactuca sativa L.) in organic growth, this work aimed to study the effect of organic fertilizers on chemical and biological character of soil and on lettuce productivity. The green manure velvet bean (Mucuna aterrima) received two types of management: incorporation into the soil or not incorporation. These both areas received the solid organic fertilizer bovine manure, commercial organic fertilizer and organic compound and a control treatment without fertilization. The samples were obtained at the time of management of velvet bean, and at 15, 55 and 105 days after this process, representing the moments of the distribution of fertilizes, plant and harvest of lettuce, respectively. Were evaluated chemical and biological characteristics of soil, and agronomic characteristics of the lettuce cultivar Vanda. The results showed that the commercial organic fertilizer followed by the organic compound, provided that highest amorents of organic matter, P, K, Ca, Mg, CTC, pH and V, and the two types of management of velvet bean, incorporated or not has similar results. For microbial biomass, better results were fownd with velvet bean not incorporated into soil plus treatment with organic compound. The population of bacteria was increased in the area where velvet bean was incorporated, except for the treatment with organic compound. For fungi, not incorporated velvet bean associated with commercial organic fertilizer provided the higher rate of population. The incorporation of velvet bean associated with organic compound increased the population of mycorrhizal fungi. The bovine manure associated with the incorporation of velvet bean and treatment with organic compound without incorporation of velvet bean increased the population of saprophytic nematodes. The area of aerial part, number of leaves and total fresh and dry weights of aerial part of lettuce, higher values were observed without the incorporation of velvet bean associated with the use of commercial organic fertilizer or bovine manure treatmentes / Em função da carência de informações técnicas sobre adubação da cultura da alface (Lactuca sativa L.), em sistema de cultivo orgânico, este trabalho teve o objetivo de estudar práticas mais convenientes de adubação que auxiliem na manutenção da fertilidade do solo e produtividade da cultura. O delineamento experimental foi o de blocos ao acaso em parcelas subdivididas onde o adubo verde mucuna preta (Mucuna aterrima) recebeu dois tipos de manejo: incorporação ao solo seguida do levantamento de canteiros, e não incorporação. As subparcelas receberam os adubos orgânicos sólidos: esterco bovino, adubo orgânico comercial e composto além da testemunha sem adubação, com cinco repetiçiões, sendo utilizada a alface cultivar Vanda. As épocas de avaliação ocorreram no momento do manejo da mucuna, e aos 15, 55 e 105 dias após esse manejo, representando momentos da distribuição dos adubos, plantio e colheita da alface, respectivamente. Foram avaliadas características químicas e biológicas do solo, e características fitométricas da planta. Pelos resultados observou-se que o adubo orgânico comercial seguido do composto, foram os que proporcionaram índices superiores de matéria orgânica, P, K, Ca, Mg, CTC, V e pH, e o manejo diferenciado do adubo verde, com ou sem incorporação possibilitou resultados semelhantes. Para a biomassa microbiana, resultados superiores foram observados com a mucuna sem incorporação e o tratamento com composto. A população de bactérias foi aumentada para o manejo da mucuna com incorporação, exceto para o tratamento composto. Para fungos, o manejo sem incorporação associado ao adubo orgânico comercial proporcionou maiores índices populacionais. O manejo com incorporação associado ao composto se mostraram favoráveis para aumento da população de fungos micorrízicos. O esterco bovino associado a incorporação da mucuna e o tratamento com composto sem incorporação da mucuna aumentaram a população de nematóides saprófitas. Para a área de projeção de saia, número de folhas e massa fresca e seca totais da parte aérea, foram observados valores superiores sem a incorporação da mucuna preta e para os tratamentos adubo orgânico comercial e esterco bovino

This diploma thesis deals with research and study of soil impedance measurement and soil resistivity. Currently, the issue of measuring and determining soil resistance is ensured through the gradual measurement of certain soil parameters at individual points of the surface (or depth of the ground). This thesis focuses on the idea of measuring soil resistance on a certain area using a network of electrodes through a suitably designed test, measurement and evaluation system. Such an approach may find application in the need to determine soil parameters (such as resistivity) on a specific demarcated area (or depth). The prospect of such an application can be seen in saving time, energy and money needed to measure the soil resistivity of a certain area (as opposed to gradual point measurements). The configuration possibilities of OMICRON CPC 100 measuring instrument were used for the design and implementation of the measuring system for the mentioned purpose. The work is completed by verification of the proposed solution by real measurement with evaluation of the results.