Made available in DSpace on 2015-03-26T13:46:59Z (GMT). No. of bitstreams: 1 texto completo.pdf: 2273628 bytes, checksum: 5b1679c5e47e66a87032a8293b4946e0 (MD5) Previous issue date: 2011-08-15 / Swine meat is one of main sources of animal protein consumed in the world and strong demand for swine meat in the Asian continent has stimulated the development of the internal market, however, has been demanded a production system aimed at animal welfare and organic production. Even though, in given situations intestinal parasites remain a problem for breeding these animals, especially when this is done through the extensive management system. Among these, stands out the parasitic nematodes, Ascaris suum and Oesophagostomum spp, whose infective forms are present in the environment. By the way, the objectives of this study were to evaluate the ovicidal activity of fungus Pochonia chlamydosporia on A. suum eggs and predatory activity of the Duddingtonia flagrans, Monacrosporium sinense and Artrhobotrys robusta on infective forms of Oesophagostomum spp. For this purpose, four experimental trials were set up, where it was evaluated (in vitro) activity of these fungi on the infectious forms of these nematodes and (in vivo) test which evaluated the ability of P. chlamydosporia and D. flagrans to support the passage through the gastrointestinal tract of pigs. Isolates fungal of P. chlamydosporia were able to destroy A. suum eggs in vitro and capable of supporting the passage through the gastrointestinal tract of pigs without losing its capacity to destroy eggs. D. flagrans, M. sinense and A. robusta predated Oesophagostomum spp L3 in vitro. D. flagrans kept its predatory ability after passing through the gastrointestinal tract of pigs. Results presented in this study suggest that the fungi P. chlamydosporia and D. flagrans can be used to assist in the control of infectious forms of the nematodes A. suum and Oesophagostomum spp, respectively. / A carne suína é uma das principais fontes de proteína animal consumida no mundo e a forte demanda por carne suína no continente Asiático tem estimulado o desenvolvimento do mercado interno, entretanto, têm se exigido um sistema de produção que vise o bem estar animal e a produção orgânica. Contudo, em dadas situações as parasitoses intestinais continuam a ser um problema para criação destes animais, principalmente quando essa se faz pelo sistema de manejo extensivo. Entre estas parasitoses destacam-se os nematoides, Ascaris suum e Oesophagostomum spp, cujas formas infePigsctantes encontram-se no ambiente. Dessa forma, os objetivos do presente trabalho foram: avaliar a atividade ovicida do fungo Pochonia chlamydosporia sobre ovos de A. suum e atividade predatória do fungo Duddingtonia flagrans, Monacrosporium sinense e Artrhobotrys robusta sobre formas infectantes e Oesophagostomum spp. Para isso, foram montados quatro ensaios, onde foi avaliada a atividade in vitro dos referidos fungos sobre as formas infectantes destes nematoides e um teste in vivo onde se avaliou a capacidade de P. chlamydosporia e D. flagrans suportar a passagem pelo trato gastrointestinal de suínos. Isolados fúngicos de P. chlamydosporia destruíram ovos de A. suum no teste in vitro e foram capazes de suportar a passagem pelo trato gastrointestinal de suínos sem perder sua capacidade de destruição de ovos. D. flagrans, M. sinense e A. robusta predaram as larvas l3 de Oesophagostomum spp. nos testes in vitro. D. flagrans manteve esta habilidade após a passagem pelo aparelho gastrointestinal dos suínos. Os resultados apresentados no presente trabalho sugerem que os fungos Pochonia chlamydosporia e Duddingtonia flagrans podem ser utilizados para auxiliar no controle das formas infectantes dos nematoides Ascaris suum e Oesophagostomum spp, respectivamente.

Neste trabalho, investigamos os mecanismos da resposta imune estimulados por PAS-1 para o desencadeamento do seu efeito modulatório na inflamação alérgica pulmonar. Para tanto, camundongos C57BL/6 selvagens, IL-12-/-, IFN-<font face=\"Symbol\">g-/- e IL-10-/- foram imunizados e desafiados com OVA ou PAS-1 ou OVA + PAS-1. Ainda, camundongos C57BL/6 imunizados e desafiados com OVA receberam transferência adotiva de células CD19+, B220+, CD3+, CD4+, CD8+, CD4+CD25-, CD4+CD25+ PAS-1-primadas. Nossos resultados demonstraram que o efeito imunomodulatório de PAS-1 é mediado por IFN-<font face=\"Symbol\">g e IL-10, mas não por IL-12, e que somente camundongos que receberam células CD8+ ou CD4+CD25+ não desenvolveram inflamação pulmonar e produziram, respectivamente, IFN-<font face=\"Symbol\">g e IL-10/TGF-ß. Em conjunto, estes resultados demonstraram que o efeito imunomodulatório de PAS-1 é devido à estimulação de células T CD8+ produtoras de IFN-<font face=\"Symbol\">g e por CD4+CD25+ secretoras de IL-10 e TGF-ß. / In this work, we investigate the immune response mechanisms triggered by PAS-1 to promote its immunomodulatory effect in the lung allergic inflammation. For that, wild type, IL-12-/-, IFN-<font face=\"Symbol\">g-/- and IL-10-/- C57BL/6 mice were immunized and challenged with OVA or PAS-1 or OVA + PAS-1. Moreover, wild type C57BL/6 mice were adoptively transferred with PAS-1-primed CD19+, B220+, CD3+, CD4+, CD8+, CD4+CD25-, and CD4+CD25+ lymphocytes. Our results demonstrated that the immunomodulatory effect induced by PAS-1 is mediated by IFN-<font face=\"Symbol\">g and IL-10, but not by IL-12, and only mice which received CD8+ or CD4+CD25+ T cells did not present lung allergic inflammation and produced IFN-<font face=\"Symbol\">g and IL-10/TGF-ß, respectively. Taken together, these results demonstrated that the imunomodulatory effect induced by PAS-1 is due to stimulating CD8+ T cells, which secrete IFN-<font face=\"Symbol\">g, and CD4+CD25+ T cells, which secrete IL-10 and TGF-ß.

Monoclonal antibodies (MAbs) have been raised against $ beta$-tubulin of B. pahangi and A. suum. Anti-B. pahangi MAbs were used to investigate the heterogeneity of tubulins from nematodes and mammals. One-dimensional SDS-PAGE showed that MAbs P3D and 1B6 react with $ beta$-tubulin from a number of filarial and intestinal nematodes, but not with tubulin from protozoan and mammalian cells. Two-dimensional SDS-PAGE demonstrated that MAb P3D recognizes two isoforms of $ beta$-tubulin and 1B6 recognizes one. Limited proteolysis showed that MAb 1B6 reacted with the amino-terminal fragments and MAb P3D with the carboxyl-terminal fragments of $ beta$-tubulin. The effect of anti-B. pahangi MAbs on the viability of adult B. pahangi was assessed using MTT assay. It was found that MAbs P3D and 1B6 caused an 80% and 40% reduction respectively, in worm viability, whereas anti-chick MAb 357 or mebendazole drug had no effect. Immunogold labelling of B. pahangi demonstrated the presence of tubulin in the median and basal layers of the cuticle, hypodermal layer and somatic muscle blocks, as well as the uterus of B. pahangi. The reduction in the viability of worms may, therefore, be due to the disruption of microtubules in the body wall muscle of B. pahangi. The total MBZ binding was highest in the intestine followed by the body wall muscle and in the reproductive tract extracts of A. suum. Electron microscopy of A. suum tissues demonstrated that the tubulin content decreased from the intestine through the body wall muscle to the reproductive tract. One dimensional SDS-PAGE revealed the presence of $ alpha,$ $ beta sb1$ and $ beta sb2$ tubulin subunits in all tissues of A. suum. This data confirmed the reduction of tubulin from the intestine through the body wall muscle to the reproductive tract. Two dimensional SDS-PAGE followed by Western blotting demonstrated that $ alpha$ and $ beta$ tubulin isoform patterns are dissimilar in different tissues of A. suum. Body wall muscle, inte

PINTO, A. C. M. D. Efeito anti-inflamatório e imunomodulador de componentes de ascaris suum em artrite experimental, 2017.118 f.Tese (Doutorado em Ciências Médicas) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2017 / Submitted by Ivone Sousa (ppgcm.ufc@gmail.com) on 2017-07-21T13:27:56Z No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) / Approved for entry into archive by Erika Fernandes (erikaleitefernandes@gmail.com) on 2017-07-21T15:51:59Z (GMT) No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) / Made available in DSpace on 2017-07-21T15:51:59Z (GMT). No. of bitstreams: 1 2017_tese_acmdpinto.pdf: 10927136 bytes, checksum: 4648aa6dab4dc5fb388eac8229d92bb9 (MD5) Previous issue date: 2017-07-10 / Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease with multifactorial etiopathogenesis, involving genetic, immunological and environmental mechanisms, whose diagnosis and delayed treatment have a negative influence on the prevalence and severity of the disease. Therefore, patients residing in low-income countries should show increased morbidity and / or mortality from these diseases. Interestingly, the evolution seems to be similar, if not less severe, to that found in populations with better socioeconomic indexes. The hygienic hypothesis suggests an inverse relationship between the global distribution of autoimmune diseases and parasitic infections, where helminths tend to stimulate the development of Th2 responses and suppress Th-1 and Th-17 responses. The aim of this study was to isolate components in Ascaris suum extract and characterize its anti-inflammatory mechanism in experimental arthritis models. Ascaris suum crude extract was separated into fractions > 30 and <30 kDa. Four smaller fractions (1-4) were obtained, in the fraction <30 kDa, by native electrophoresis. In fraction 1, the ABA-1 protein was identified by mass spectrometry and after protein digestion with trypsin, seven peptides were isolated (Aba-AG). The genomic sequence of the Aba-B and Aba-F peptides was inserted into a commercial plasmid (VR2 -001-TOPO ™). Plasmids were cloned and expanded in E. coli and extraction of plasmid DNA was performed by commercial kit (Qiagen ™). Male Swiss mice received crude extract of A. suum (1mg), > 30kDa, <30kDa (50ng) or fractions 1, 2, 3 and 4 (1μL), diluted in sterile saline (200 μL), p.o., 30min before of the injection of Zy (0.1mg) i.a. Hypernociception was evaluated by the electronic von Frey and the cellular influx was evaluated in the joint fluid 6h or seven days after zymosan i.a. Swiss and Balb/c male mice, submitted to ZyA and AIA, respectively, received recombinant plasmids of Aba-B or Aba-F (50μg/30μL) at the back of each hind leg. The cell influx was evaluated 6h or seven days (ZyA) and 7h or 14 days (AIA) after the i.a stimulus. Cytokines were evaluated in the synovial fluid. Synovitis and joint damage were evaluated by H&E and safranin O, respectively, and mast cell by toluidine blue. Levels of cytokines (CXCL-1, IL-1β, IFN-γ, IL- 5, IL-6 and IL-10) were assessed by ELISA and the expression of iNOS, CD11b, F4/80 and CD206 by immunohistochemistry. The administration of fractions <30 kDa, 1 and 4 reduced hypernociception and acute and chronic cellular influx in the ZyA model. Transfection with both plasmids, Aba-B or F, significantly reduced the influx of cells in both the zymosan and acute and chronic mBSA arthritis models. Reduction of synovitis, mast cell numbers, joint damage and iNOS expression were observed. Levels of CXCL1, IFN-γ, IL-1β and IL-6 were reduced. However, IL-10 levels did not increase. Surprisingly, IL-5 levels were significantly reduced after plasmid injection. Plasmids increased the expression of M2 macrophages in the synovium. A qPCR analysis showed the expression of peptides in the thigh muscles of mice that received the plasmids. This is the first demonstration that peptide sequences derived from A. suum promote anti-inflammatory activity in arthritis models, and that it is associated with reduced release of inflammatory cytokines. Peptides promoted a change in the differentiation of macrophages in the synovial membrane at the same time, decreasing the release of IL-5. Despite the complex modulating mechanisms of helminths of inflammation, the data still support a protective effect of helminth products in chronic arthritis. / A artrite reumatoide (AR) é uma doença inflamatória autoimune crônica com etiopatogenia multifatorial, envolvendo mecanismos genéticos, imunológicos e ambientais, cujo diagnóstico e tratamento tardio influenciam negativamente na prevalência e gravidade da doença. Portanto, pacientes que residem em países com baixa renda deveriam apresentar um aumento da morbidade e / ou mortalidade por essas doenças. Curiosamente, a evolução parece ser semelhante, se não menos grave, à encontrada em populações com melhores índices socioeconômicos. A hipótese higiênica sugere relação inversa entre a distribuição global de doenças autoimunes e infecções parasitárias, onde helmintos tendem a estimular o desenvolvimento de respostas Th2, além de suprimir respostas Th-1 e Th-17. O objetivo deste estudo foi isolar componentes no extrato de Ascaris suum e caracterizar seu mecanismo antiinflamatório em modelos de artrite experimental. O extrato bruto de Ascaris suum foi separado em frações > 30 e <30 kDa. Foram obtidas quatro frações menores (1-4), na fração <30 kDa, por eletroforese nativa. Na fração 1, foi identificada a proteína ABA-1, por espectrometria de massa, e após digestão proteica com tripsina, sete peptídeos foram isolados (Aba-A – G). A sequência genômica dos peptídeos de Aba-B e Aba-F foi inserida em um plasmídeo comercial (VR2-001-TOPO ™). Os plasmídeos foram clonados e expandidos em E. coli e a extração do DNA plasmidial foi realizada por kit comercial (Qiagen™). Camundongos Swiss machos receberam extrato bruto de A. suum (1mg), >30 kDa , <30kDa (50ng) ou frações 1, 2, 3 e 4 (1μL), diluídos em salina estéril (200 μL), v.o., 30 min antes da injeção de Zy (0,1mg) i.a. Hipernocicepção foi avaliada pelo von Frey eletrônico e o influxo celular foi avaliado no fluido articular 6h ou sete dias após zymosan i.a. Camundongos Swiss e Balb/c machos, submetidos a artrite induzida por zymosan AZy e induzida por antígeno (AIA), respectivamente, receberam plasmídeos recombinantes de Aba-B ou Aba-F (50μg/30μL) na parte posterior de cada coxa traseira. O influxo celular foi avaliado 6h ou sete dias (AZy) e 7h ou 14 dias (AIA) após o estímulo i.a. Citocinas foram avaliadas no líquido sinovial. Sinovite e dano articular foram avaliados por H&E e safranina O, respectivamente, e número de mastócitos por azul de toluidina. Níveis de citocinas (CXCL-1, IL-1β, IFN-γ, IL-5, IL-6 e IL-10) foram avaliadaos por ELISA e a expressão de iNOS, CD11b, F4/80 e CD206 por imunohistoquímica. A administração das frações <30 kDa, 1 e 4 reduziu a hipernocicepção e influxo celular agudo e crônico no modelo de AZy. A transfecção com ambos os plasmídeos, Aba-B ou F, reduziu significativamente o influxo de células em ambos os modelos de artrite por zymosan e mBSA agudo e crônico. Foram observados a redução da sinovite, número de mastócitos, do dano articular e expressão de iNOS. Níveis de CXCL1, IFN-γ, IL-1β e IL-6 foram reduzidos. No entanto, os níveis de IL-10 não aumentaram. Surpreendentemente, os níveis de IL-5 foram significativamente reduzidos após a injeção de plasmídeos. Plasmídeos aumentaram a expressão de macrófagos M2 na sinóvia. Uma análise quantitativa por PCR mostrou a expressão de peptídeos Aba-B e Aba-F nos músculos da coxa de camundongos que receberam os plasmídeos. Esta é a primeira demonstração de que sequências peptídicas derivadas de A. suum promovem a atividade anti-inflamatória em modelos de artrite, e que está associada à redução da liberação de citocinas inflamatórias. Esses peptídeos promoveram mudança na diferenciação de macrófagos na membrana sinovial ao mesmo tempo, diminuindo a liberação de IL-5. Apesar dos complexos mecanismos de modulação de helmintos de inflamação, os dados suportam ainda um efeito protetor dos produtos de helmintos em modelos de artrite crônica.

Submitted by Helena Bejio (helena.bejio@unioeste.br) on 2017-11-16T18:52:34Z No. of bitstreams: 2 Fábio HR Silveira 2016.pdf: 2555885 bytes, checksum: 29051e06e2f268f821a9b563e9878402 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-11-16T18:52:34Z (GMT). No. of bitstreams: 2 Fábio HR Silveira 2016.pdf: 2555885 bytes, checksum: 29051e06e2f268f821a9b563e9878402 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-03-14 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This study aims to assess the farm size effect and parturition order on the occurrence and the logarithm of the egg count per gram of endo- stool in commercial sows housed in the maternity and pregnancy in situated farms in West micro-region Paraná. The 43 Production Units Piglets, set in eight micro-regions, classified into four farm sizes: small (100 to 250 arrays), medium (251-510 arrays), large (511-1000 arrays) and very large (more than 1,000 dies ) and three orders calving up to two deliveries, 3 to 5 and more than 5 deliveries. The feces samples were processed through flotation technique saturated salt solution. Statistical analysis was based on the theory of generalized linear models, the observed data variables expressed by binary values were adjusted to the binomial and the normal distributions. The existing significance farm size (TG), birth order (PO), interaction between TG and OP was verified by the deviance analysis Of the 1,596 fecal samples, 4.64% were positive for Ascaris suum, 0, Trichuris suis to 56% and 8.27% for oocysts of coccidia. Properties with small and medium farm size have a higher percentage of parasites when compared with large and very large, especially in farrowing order between zero and two deliveries. The younger females removed a greater amount of Ascaris suum and Trichuris suis. / O presente estudo tem por objetivo avaliar o efeito de tamanho de granja e ordem de parição sobre a ocorrência e o logaritmo da contagem de ovos por grama de fezes de endoparasitas em matrizes suínas comerciais alojadas na maternidade e gestação em granjas situadas na microrregião do Oeste do Paraná. As 43 Unidades Produtoras de Leitões, inseridas em oito microrregiões, classificadas em quatro tamanhos de granja: pequena (100 a 250 matrizes), média (251 a 510 matrizes), grande (511 a 1.000 matrizes) e muito grande (mais que 1.000 matrizes) e em três ordens de parição: até 2 partos, 3 a 5 e mais que 5 partos. As amostras de fezes foram processadas por meio da técnica de flutuação em solução saturada de sal. A análise estatística foi realizada com base na teoria dos modelos lineares generalizados, os dados observados de variáveis expressas por valores binários foram ajustados às distribuições binomial e normal. A significância existente de tamanho de granja (TG), ordem de parto (OP), interação entre TG e OP foi verificada por meio da análise de deviance, Das 1.596 amostras fecais coletadas, 4,64% foram positivas para Ascaris suum, 0,56% para Trichuris suis e 8,27% para oocistos de coccídeos. As propriedades com tamanho de granja pequeno e médio possuem uma porcentagem mais elevada de parasitas quando comparada com as grandes e muito grandes, principalmente nas de ordem de parição entre zero e dois partos. As fêmeas mais novas eliminaram uma maior quantidade de ovos de Ascaris suum e Trichuris suis.

Serotonin (5-hydroxytryptamine, 5-HT) has been shown to activate carbohydrate metabolism in adult female Ascaris suum. Serotonin may be either absorbed directly from the environment or synthesized de novo from the absorbed L-tryptophan in adult female A. suum. The enzymes necessary for the synthesis of 5-HT have been identified in both intestine and muscle tissues. The serotonin absorbed from the environment is obtained either from the host's gastrointestinal contents or from the 5-HT producing bacteria in the intestine of A. suum. Numerous 5-HT producing bacteria were identified in the intestinal microflora. The physiological contributions of 5-HT producing bacteria to the 5-HT level, turnover and binding sites in the intestinal tissue of A. suum were investigated.

The studies described in this dissertation examine the effects of F-2,6-P2 and AMP or phosphorylation on the kinetic mechanism of d-PFK. The effect of varied pH on the activation by F-2,6-P2 is also described.

No description available.

BACKGROUND: Both endo- and ectoparasites pose a great challenge to the health of pigs worldwide, placing a significant burden on low-resource countries where veterinary care is minimal. As part of a larger clinical trial assessing the use of ivermectin (IVM) mass drug administration to humans and pigs for the control of malaria vectors in the Mopeia district in Mozambique, a longitudinal study to assess the impact of IVM administration on pig health was performed. METHODS: Beginning in March 2022, IVM was administered to pigs in the intervention area once a month for three consecutive months. Seventy pigs from the treatment group and 70 pigs from the control group were randomly selected for inclusion in the study. Fecal samples were collected monthly for three months and analyzed for the presence of strongyle eggs, strongyle eggs in the larval stage (strongyles – larval) and Ascaris suum using the modified McMaster test. Fecal samples were also collected two weeks after each dose of IVM was given to pigs in the treatment group for determination of a fecal egg reduction count. Juvenile pigs were measured twice a month for the first 3 months of the study, then once monthly for another three months. Visual exam for ectoparasites was performed on all pigs for the presence of ticks, lice or scabies at the same time points. RESULTS: Overall, 55% [95% CI: 48-62%] of pigs were positive for Ascaris suum, 95.2% [95% CI: 91-98%] were positive for strongyle eggs, and 72.5% [95% CI: 65.5-79%] were positive for strongyle – larval. A significant difference in the ivermectin treatment group was only seen one month after the second dose of ivermectin was administered: pigs in the treatment group had a 23.6% lower prevalence of strongyles (p = 0.003) and 18% lower prevalence of strongyles – larval (p = 0.03). Pigs in the treatment group also had lower EPG for Ascaris suum (diff = 102 EPG; p = 0.006), strongyles (diff = 642 EPG; p < 0.001), and strongyles - larval (diff = 217 EPG; p < 0.001). Analysis of covariance regression found no significant difference(P>0.05) in average daily weight gain (ADG) between the treatment and control groups. CONCLUSION: IVM delivered once monthly for three months has a small impact on pig health. To counteract the multiple health challenges pigs face in these settings, different dosing schedules along with education on husbandry issues related to nutrition and sanitation should be investigated in order to maximize impact on pig health. / Master of Science / A study was conducted in rural Mozambique in the district of Mopeia to determine the effectiveness of ivermectin against common parasites of swine when administered to pigs. The study began in March of 2022, at the beginning of rainy season, and ivermectin was given to pigs once a month for three months. Pigs were visited twice a month for the first three months, and then once a month for another three months. At various time points, fecal samples were collected, pigs were examined for evidence of ectoparasites (ticks, lice and scabies infestation), and young pigs were measured to determine their rate of growth. Fecal samples were analyzed for the presence of common internal parasites (endoparasites) affecting pigs in the area. The burden of endo and ectoparasites was estimated before any ivermectin was administered, and then compared in treated and untreated pigs over the course of the study. Similarly, the effect of ivermectin on growth rates in young animals was determined. The results of this study found that there was a high burden of common endoparasites in pigs in the Mopeia district, which was only minimally affected by the use of ivermectin delivered once a month. Among the treated pigs, a fecal egg count reduction test suggests that these parasites are potentially resistant to ivermectin, although other issues may be responsible for these results. The burden of ectoparasites was generally low (<10%), with ivermectin only significantly reducing the prevalence of ticks. Young animals that received ivermectin had a 15% increase in their growth rate, but this was not statistically significant. In conclusion, the use of ivermectin once a month for three months in pigs, as part of a malaria intervention, has some minimal positive health effects on treated pigs. Given the poor management practices, poor nutrition and lack of veterinary care in these pigs, it is likely that to have a greater impact on pig health, ivermectin will need to be delivered under a different dosing schedule and alongside owner education on pig management practices.

Antígenos de alta massa molecular (PI) do extrato de Ascaris suum exercem efeito supressor sobre a resposta imune a antígenos heterólogos. PI atua diretamente sobre as DCs, diminuindo a expressão das moléculas coestimuladoras, MHC de classe II e assim, a proliferação de linfócitos T. Esse efeito é independente de TLR2, TLR4 e da molécula MyD88. Nesse trabalho estudamos a participação dos receptores DC-SIGN e MR, na modulação da atividade das DCs. PI contém oligossacarídeos N-ligados com cadeias de alta manose e do tipo complexa e contém resíduos de fosforilcolina. Os componentes do PI contendo as cadeias glicosídicas N-ligadas inibem a maturação de DCs incubadas com LPS. Receptores DC-SIGN e MR estão envolvidos no reconhecimento e internalização dos componentes do PI pelas DCs. O bloqueio desses receptores foi capaz de abolir o efeito inibitório do PI sobre as DCs e a resposta proliferativa de linfócitos T in vitro. Portanto, os resultados mostram a participação do DC-SIGN e MR no reconhecimento de componentes glicosilados do PI e na sua ação imunomoduladora. / High molecular weight components (PI) of Ascaris suum extract exert suppressive effect on the immune response to OVA. PI exert direct effect on DCs, decreasing the T lymphocyte proliferation. This effect is independent of TLR2 and 4 as well as MyD88 molecule. In this work we studied the glycoconjugates in PI and the participation of DC-SIGN and MR, in the modulation of the functional activity of DCs. PI components contain high mannose- and complex-type N-linked oligosaccharides and phosphorylcholine residues. PI components containing N-linked glycans inhibited the DCs maturation induced by LPS. The previous incubation of DCs with mannan, anti-DC-SIGN and anti-MR antibodies abolished the modulatory effect of PI on the DCs maturation. It was also observed that the blockage of DC-SIGN and MR in DCs reversed the inhibitory effect of PI in the in vitro T cells proliferative response. Taking together these results show the involvement of DC-SIGN and MR in the recognition of glycosylated components of PI by DCs and in its modulatory effect.