In the late spring of 2008, the Chevron-led Gulf of Mexico Gas Hydrate Joint Industry Project (JIP) expects to conduct an exploratory drilling and logging campaign to better understand gas hydrate-bearing sands in the deepwater Gulf of Mexico. The JIP Site Selection team selected three areas to test alternative geological models and geophysical interpretations supporting the existence of potential high gas hydrate saturations in reservoir-quality sands. The three sites are near existing drill holes which provide geological and geophysical constraints in Alaminos Canyon (AC) lease block 818, Green Canyon (GC) 955, and Walker Ridge (WR) 313. At the AC818 site, gas hydrate is interpreted to occur within the Oligocene Frio volcaniclastic sand at the crest of a fold that is shallow enough to be in the hydrate stability zone. Drilling at GC955 will sample a faulted, buried Pleistocene channel-levee system in an area characterized by seafloor fluid expulsion features, structural closure associated with uplifted salt, and abundant seismic evidence for upward migration of fluids and gas into the sand-rich parts of the sedimentary section. Drilling at WR313 targets ponded sheet sands and associated channel/levee deposits within a minibasin, making this a non-structural play. The potential for gas hydrate occurrence at WR313 is supported by shingled phase reversals consistent with the transition from gas-charged sand to overlying gas-hydrate saturated sand. Drilling locations have been selected at each site to 1) test geological methods and models used to infer the occurrence of gas hydrate in sand reservoirs in different settings in the northern Gulf of Mexico; 2) calibrate geophysical models used to detect gas hydrate sands, map reservoir thicknesses, and estimate the degree of gas hydrate saturation; and 3) delineate potential locations for subsequent JIP drilling and coring operations that will collect samples for comprehensive physical property, geochemical and other analyses

Recent observations have identified a new species of leucothoid amphipod, Leucothoe “sp. F,” associated with the sponge Cliona varians. This project examined the relationship between this amphipod and its sponge host at three sites in the Florida Keys with differing hydrodynamic regimes. Ninety-eight sponge samples with a total of 2,030 amphipods were collected between December 2011 and September 2012. Leucothoe “sp. F” is currently a common species in the Florida Keys strongly associated with C. varians; its distribution strongly coincides with open tidal currents from the Gulf of Mexico. Seasonality, depth, and tidal regimes not only influence population dynamics and sexual characteristics of Leucothoe “sp. F,” but also the abundance and volume of its host.

The Veracruz Reef System (VRS) is located in the southwestern Gulf of Mexico. It is comprised of 28 coral reefs in various stages of development and conservation. They are protected under the Parque Nacional Sistema Arrecifal Veracruzano National Park created in 1992. There are many threats to the reefs of the VRS, including the Port and city of Veracruz, which hosts half a million inhabitants and Mexico’s oldest active port. The inhabitants of Veracruz have used reef resources for thousands of years, as evidenced in archaeological sites on Sacrificios island, and constructions throughout the city, most notably in the San Juan de Ulúa Fort which was built entirely of coral skeletons. Despite the usage and protection given under the National Park, there is relatively little known about the health and condition of the stony corals in the System. There has only been one large scale study of 21 reefs conducted in the VRS in the late 1980’s. Since then, the National Park was created and 28 reefs are now recognized. This study performed point-intercept transects on 24 of these reefs including five reefs added to the official list in 2012. Point-intercept transects were surveyed at 63 sites between 2007 and 2014. Percent cover was calculated for seven functional groups. Additionally, demographic data of a subset of individual stony coral colonies were assessed on each transect. The functional group with the greatest cover in the VRS was crustose coralline algae (mean ± S.E.: 28.9% ± 1.97), stony corals had the second highest cover (21.5% ± 1.24). The Jamapa river divides the VRS into two groups the Veracruz group to the North and the Anton Lizardo group to the south of the river mouth. The Veracruz group had lower crustose coralline algae cover (28.1% ± 2.71) and coral cover (17.8% ± 1.55) than the Anton Lizardo group (29.6% ± 2.87 CCA and 25.3% ± 1.86 coral cover). The highest average coral cover on a reef was recorded at Ahogado Chico (45.5% ± 5.58), and the highest cover recorded on a single transect was 70% at Santiaguillo reef. The lowest coral cover was recorded at the fringing reefs on the north of the VRS, Punta Gorda and Punta Brava which had less than 1% coral cover. Coral colonies averaged 69.1 cm ± 3.10 in length at the VRS, 56.8 cm ± 2.98 in the Veracruz group and 81.7 cm ± 5.11 in the Antón Lizardo group. Old partial mortality was 25% ± 1.05 overall and similar between groups, recent partial mortality was 1.2% ± 0.21 and 1% at both groups. Disease prevalence was 3.9% for the VRS, 2.9% ± 0.88 in the Veracruz group and 4.9% ± 1.11 in the Antón Lizardo group. Overall, these reefs are faring slightly better than other reefs in the Caribbean having higher coral cover and larger colonies. However, the great variability in the health and condition of these reefs demands added attention and clear management goals to ensure their persistence in the face of ever growing threats. It is important to decrease the sources of stress, such as construction and poor waste water management in the area, better regulate fishing and approach a watershed wide management plan which takes into account upstream effects from the rivers that discharge into the Veracruz Reef System.

Climate has historically been recognized as an influence on sediment flux and deposition. The North American Monsoon is suggested as the forcing mechanism of deltaic progradational events of the Rio Grande River delta. Interpretations of reflection seismic profiles reveal that eustatic rise in sea-level from the Last Glacial Maximum to present is accompanied by several regressional events of the Rio Grande delta 5.5, 9.5, and 11.5 ka BP. Much of the migration of depositional facies within a delta system is forced by hinterland tectonics and base-level rise and fall. However, we suggest that the movement of facies within the Rio Grande delta system represent climate forcing as the most dominant influence on sediment deposition during this short time period. While dominance of climate influence is possible, the sensitivity of an increase in monsoon precipitation and its effect on sediment flux has not yet been tested. We test monsoonal effects using relationships between sediment flux, river discharge, and precipitation. Heavy water management and withdrawal and complexity of precipitation timing and events within the region make the relationship between precipitation and sediment flux difficult to quantify using modern data sources. Therefore, it is necessary to numerically simulate stream discharge to test potential sensitivities of the system to monsoonal precipitation using a stream discharge model. Precipitation input into the stream discharge model is gathered from a suite of climate model simulation outputs. Suspended sediment flux is derived from the outputs of the flow models using empirically derived sediment rating curves. Results of sediment modeling show that increased precipitation during the monsoon months of July-September, 6 ka BP increased monthly suspended sediment flux by 79 percent. The suite of climate models does not include 9 or 11 ka BP, but we suggest the monsoon may have been stronger during this time based on greater received insolation at these times. This study also shows that duration and intensity of monsoonal precipitation events can more greatly affect stream discharge and sediment flux than increased precipitation with constant storm intensity.

No description available.

River discharge leads to extensive phytoplankton blooms often observed in ocean color satellite images to extend far into the open ocean as high chlorophyll plumes. We investigated diversity, distribution and ecology of phytoplankton populations in the Mississippi River plume, both spatially and in the water column using molecular tools. A method was developed for the quantification of diatom/pelagophyte rbcL (large subunit of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase) mRNA using quantitative PCR and applied to cultures and in the plume. The vertical structure of phytoplankton species in the Mississippi River plume was described by flow cytometry, pigments, rbcL mRNA and rbcL cDNA libraries. High productivity in the plume was associated with a large population of Synechococcus and elevated levels of cellular form IA rbcL mRNA. rbcL cDNA libraries indicated two vertically separated clades of Prochlorococcus (high-light and low-light adapted) in addition to a diverse group of prymnesiophytes and a microdiverse clade of prasinophytes, which may have dominated the SCM (Subsurface Chlorophyll Maximum). In situ sampling and satellite image analysis were used to estimate that the plume accounted for 41% and 13% of all surface water column ix productivity in the oligotrophic Gulf of Mexico, while covering less than 3% of its area. Coastally the plume is dominated by diatoms, which are replaced by a bloom of Synechococcus as the plume moves offshore. Diatoms as indicated by pigments and rbcL clone libraries again dominated the offshore, least productive plume. 15N uptake measurements indicated that rapid recycling of ammonium despite higher levels of nitrate primarily drives production in the offshore plume. rbcL mRNA levels and photosynthetic capacity displayed strong diel patters in three out of four time series sampled during the GRIST (Geochemical Rate/mRNA Integrated Study). In addition it was demonstrated that transcriptional regulation of the global nitrogen regulatory protein NtcA in Synechococcus WH7803 may involve a small cis-encoded anti-sense mRNA. Methods for the generation of large insert BAC (Bacterial Artificial Chromosome) from cultures and the environment were refined. Partial sequencing and genomic comparison of an ntcA containing BAC clone obtained from Synechococcus WH7803 indicated that ntcA is not part of a larger nitrogen assimilation operon in cyanobacteria.

No description available.

Gas hydrate formation was studied in Gulf of Mexico (GOM) sediments. Sediments studied were from six-meter long cores from Mississippi Canyon Block 118 and a 27-meter core from a cruise in 2002 of the Marion Dufresne. These sediments retained their in situ seawater before testing. Hydrate formation rate and induction times were measured. The hydrate memory effect was studied in GOM sediments with and without in situ seawater. Hydrate induction time was short when in situ seawater was present. Bioproducts adsorbed on particles in the sediments are postulated to shorten the induction times by maintaining seawater structuring around coated particles. Hydrate nucleation was studied by Dynamic Light Scattering and Scanning Electron Microscopy. Particles around 50 to 100 nm nucleated hydrate formation. These small nucleating particles appeared to be clays or surfactant molecules and interactions thereof. Hydrate capillaries were studied and found to be at least 100 nm in diameter because the sediment nucleating particles with bioproducts diffused through the hydrate capillaries. Large complexes of nontronite smectite clay and Emulsan, an anionic biosurfactant, were found to facilitate hydrate formation. It was determined that Emulsan entered the interlayer of nontronite. The clay contents of the GOM sediments were determined. All sediments contained smectite, illite, chlorite, and kaolinite in different proportions. The study gave new insight into the gas hydrate formation mechanism in seafloor sediments.

No description available.

A field survey was conducted following the Deepwater Horizon blowout and it was noted that resulting coastal petroleum deposits possessed distinct geometries, ranging from small tar balls to expansive horizontal oil sheets. A laboratory study evaluated the effect of oil deposit geometry on localized gradients of electron acceptors and microbial community composition, factors that are critical to accurately estimating biodegradation rates. One-dimensional top-flow sand columns with 12-hour simulated tidal cycles compared two contrasting geometries (isolated tar "balls" versus horizontal "sheets") relative to an oil-free control. Significant differences in the effluent dissolved oxygen and sulfate concentrations were noted among the columns, indicating presence of anaerobic zones in the oiled columns, particularly in the sheet condition. Furthermore, quantification of genetic markers of electron acceptor and catabolic conditions via quantitative polymerase chain reaction of dsrA (sulfate-reduction), mcrA (methanogenesis), and cat23 (oxygenation of aromatics) genes in column cores suggested more extensive anaerobic conditions induced by the sheet relative to the ball geometry. Denaturing gradient gel electrophoresis similarly revealed that distinct gradients of bacterial communities established in response to the different geometries. Thus, petroleum deposit geometry impacts local redox and microbial characteristics and may be a key factor for advancing attenuation models and prioritizing cleanup. / Master of Science