Thesis (M.A.)--Miami University, Dept. of Comparative Religion, 2004. / Title from second page of PDF document. Includes bibliographical references (p. 64-77).

Syftet med denna studie är att undersöka vad som skett i överföringen av Roald Dahls bok Kalle och chokladfabriken (1984) till filmatiseringen med samma namn regisserad av Tim Burton (2005). Det som mer specifikt fokuseras på i analysen är vad som skett med karaktärerna, om genren har påverkats och vad som har lagts till och tagits bort. Vidare diskuterar denna studie, med utgångspunkt i läroplan för grundskola, förskoleklass och fritidshem (2011), de värden och normer som framkommer i verken. För att se hur dessa skulle kunna ligga till grund för värdegrundskopplad undervisning i mellanstadiet. Detta har undersökts genom en komparativ analys av de båda verken. Det vi har kommit fram till är att en av karaktärerna i överföringen till film har fått en mer fördjupad funktion. Detta är något som påverkar filmens genre och därigenom också hela filmens budskap. Filmen har en tydlig psykologisk vinkel som inte finns med i boken. De andra karaktärerna är förhållandevis lika i adaptionen. De normbrytande och säregna karaktärsdragen gör detta till ett bra underlag för diskussion med elever i mellanstadiet.

Conserved residues in protein are crucial for maintaining structure and function, either by direct involvement in chemistry or indirectly, by being essential for folding, stability and oligomerisation and are mostly clustered near active sites. The variability of sequences of the same protein from diverse organisms is a reflection of the selective pressures of evolution. Sequence conservation analysis with 3397 bacterial triosephosphate isomerase (TIM) sequences using Plasmodium falciparum (Pf) TIM as template, showed full conservation of ten residues, K12, T75, H95, E97, C126, E165, P166, G209, G210 and G228. The integrity of the enzyme active site, which lies near the dimer interface, makes TIM an obligatory dimer. Attempts to engineer active monomeric TIM have not been successful. The present study assesses the effects of mutations at fully conserved position 75 (Thr) and the highly conserved position 64 (Q: 3011, E: 383) near the dimer interface, using the recombinant Plasmodial enzyme. Residue 64, Gln in Pf, and T75 interact with the catalytic E97 and K12, respectively. Preliminary analysis of available crystal structures showed that Gln 64 takes part in a single intersubunit interaction and maintains the obligatory strained backbone angles of the catalytic K12 residue, while Thr 75 is involved in four intersusunit hydrogen bond interactions. This led to the hypothesis that mostly, Gln at position 64 is crucial for enzyme activity and Thr at position 75 for the integrity of the dimer. Biophysical and kinetic data are reported for four T75 (T75S/V/C/N) and two Q64 (Q64N/E) mutants. The major findings revealed that the mutations at position 64 have a significant effect on dimer integrity with a 1000 fold increase in the dimer dissociation constant compared to the wild type enzyme, while dimer stability was unimpaired for the T75 mutants. Concentration dependence of activity yielded an estimate of dimer dissociation constant (Kd) values (Q64N 73.7±9.2 nM and Q64E 44.6±8.4 nM). Enzyme activity values of the T75 mutants are comparable to the wild type, except for T75N which shows a 4-fold drop in activity. All four T75 mutants show a dramatic fall in activity between 35 °-45 °C. Crystal structure determination of the T75S/V/N mutant offers insights into the variation in local interactions with T75N showing the largest changes. These results were unanticipated emphasising the uncertainties involved in inferring functional and structural role for individual residues based only on analysis of interactions observed in crystal structures. Nanospray ionisation mass spectrometric studies has also been used to probe the oligomeric properties of the three mutant proteins Q64N, Q64E and T75S and the wild type enzyme in the gas phase. The gas phase distributions of dimeric and monomeric species have been examined under a wild range of collision energies (40 – 160 eV). The order in the gas phase, PfTIM wild type > T75S > Q64E ~ Q64N, together with the solution phase experiments described above establish the importance of Q64 and T75 in influencing stability and activity. Inhibition studies with a 27 residue synthetic dimer interface peptide and the Q64 mutants establish that the interaction between the protein and the peptide was facilitated in the case of monomeric species.

C-uppsatsen baseras på en fallstudie över ABB Communication Centers internkommunikation, den övergripande kommunikativa enheten på ABB Sverige AB. Syftet är att beskriva informationsdesignens roll i såväl den dagliga verksamheten som den övergripande inställningen till det internkommunikativa arbetet. Samt jämföra teorier inom organisations- och kommunikationsvetenskap samt informationsdesign och resultaten från den empiriska undersökningen. / This thesis is based on a case study carried out at ABB Communication Center, the comprehensive department for communication within ABB Sweden AB. The purpose of the work is to describe how internal communications are accomplished in a real organisation and which role information design has in the process, and then to compare this with the theories within communications, organisation and information design and to evaluate the results.

Abstract The aim of this PhD-study was to address some of the overlapping bottlenecks in protein engineering and metagenomics by developing or applying new tools which are useful for both disciplines. Two enzymes were studied as an example: Triosephosphate Isomerase (TIM) and Uridine Phosphorylase (UP). TIM is an important enzyme of the glycolysis pathway and has been investigated via means of protein engineering, while UP is a key enzyme in the pyrimidine-salvage pathway. In this thesis TIM was used to address protein engineering aspects, while UP was used in regards to some metagenomic and bioinformatic aspects. The aspects of a structural driven rational design approach and its implications for further engineering of monomeric TIM variants are discussed. Process development based on a new technology, EnBase®, addresses the relative instability of new variants, compared to its ancestors, for further studies. EnBase® is then applied for the production of 15N isotope labeling of a monomeric TIM variant, A-TIM. Systematical function- and engineering studies on dimeric TIM and monomeric TIM in regards to the hinges of the catalytic loop-6 were conducted to investigate enzyme activity and stability. Both the A178L and P168A were proposed to induce loop-6 closure, a wanted feature for A-TIM variants. The P168A mutants are hardly active, but gave great insight into the catalytic machinery, while the A178L mutants did induce partial loop-6 closure, however in addition, monomeric A178L was destabilized. Homology driven genome mining and subsequent isolation- high throughput (HTP) overexpression of a thermostable UP from the Archaea Aeopyrum pernix was carried out as an example for the production of recombinant proteins. In addition an alternative kinetic method to study the kinetics of UP by means of NMR directly from cell lysate is discussed. The combination of expression libraries and EnBase® in a HTP manner may relieve up the gene-to-product bottleneck. The structural aspects of A. pernix UP are explored by means of simple bioinformatic tools in the last section of this thesis. A thermostable, truncated version of UP was created and its use for protein engineering in the future is explored. The long N-terminal and C-terminal ends of A. pernix UP seem to be involved in stabilizing the dimeric and hexameric structures of UP. However, deletion of the N-terminal end of A. pernix UP yielded a thermostable protein. Overall, the finding in regards to process optimization and HTP expression and optimization and the underlying methods used in the TIM studies and the UP studies are interchangeable.

Interaction of various components in rotating machinery like gearboxes may generate excitation forces at various frequencies. These frequencies may sometimes overlap with the frequencies of the forces generated by other components in the system. Conventional vibration spectrum analysis does not attenuate noise and spectral frequency band overlapping, which in many applications masks the changes in the structural response caused by the deterioration of certain components in the machine. This problem is overcome by the use of time domain averaging (dsynchronous averaging). In time domain averaging, the vibration signal is sampled at a frequency that is synchronized with the rotation of the gear of interest and the samples obtained for each singular position of the gear are ensemble-averaged. When sufficient averages are taken, all the vibration from the gearbox, which is asynchronous with the vibration of the gear, is attenuated. The resulting time synchronously averaged signal obtained through this process indicates the vibration produced during one rotation of the monitored gear. This direct time domain averaging process essentially acts as a broadband noise synchronous filter, which filters out the frequency content that is asynchronous with the vibration of the gear of interest provided that enough averages are taken. The time domain averaging procedure requires an enormous amount of vibration data to execute, making it very difficult to develop online gearbox condition monitoring systems that make use of time domain averaging to enhance their diagnostic capabilities since data acquisition and analysis cannot be done simultaneously. The objective of this research was to develop a more efficient way for calculating the time domain average of a gear vibration signal. A study of Artificial Neural Networks (ANNs) and Support Vector Machines (SVMs) was conducted to assess their suitability for use in time domain averaging. Two time domain averaging models that use ANNs and SVMs were developed. Model 1 uses a single feedforward network configuration to map the input which are rotation synchronized gear vibration signals to the output which is the time domain average of the gear vibration signal, using only a section of the input space. Model 2 operates in two stages. In the first stage, it uses a feedforward network to predict the instantaneous time domain average of the gear vibration after 10 inputs (10 rotation synchronized gear vibration signals) to predict the instantaneous average of the gear rotation. The outputs from the first state are used as inputs to the second stage, where a second feedforward network is used to predict the time domain average of the entire vibration signal. When ANNs and SVMs were implemented, the results indicated that the amount of gear vibration date that is required to calculate the time domain average using Model 1 can be reduced by 75 percent and the amount of gear vibration data that needs to be stored in the data acquisition system when Model 2 is used can be reduced by 83 percent. / Dissertation (M Eng (Mechanical and Aeronautical Engineering))--University of Pretoria, 2006. / Mechanical and Aeronautical Engineering / unrestricted

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In this essay i examine how five persons from the city of Piteå who have moved to big cities in Sweden identifies with different places. My aim is to show a more ​heterogeneous image of Norrland and its inhabitants. In the essay I examine how my informants imbue certain words and practises, such as dialects or the act of visiting their hometown, with meaning and how this affects their identification towards the places that they call home. In this essay I understand places not as merely physical and material but also as socially constructed. Therefore I see the construction of places as an important aspect of my informants identification.

Bostadsmarknaden i Sverige blir allt mer osäker. För studenter som söker bostad på sin studieort har det blivit vanligare att man måste hyra i andra hand. Vad innebär detta för studenterna? Hur kan de bo och skapa ett hem inom dessa villkor på en bostadsmarknad med tillfälliga boendeformer? Följande uppsats har att analyserat hur man kan skapa ett hem på denna typ av bostadsmarknad genom att tillämpa Tim Ingolds begrepp ”dwelling perspective”. Utifrån Rolands Paulsen bok ”En studie i oro” (2020) tar uppsatsen även upp en diskussion om hur vi hanterar att leva i en samtid där oro och en känsla av otrygghet är ständigt närvarande. Genom ett antropologiskt perspektiv på hemmet och att bo är denna uppsats en analys av hur studenter bor och skapar ett hem på en osäker bostadsmarknad.

Hepatitis B virus (HBV) vaccination is recommended for individuals with hepatitis C virus (HCV) infection given their shared risk factors and increased liver-related morbidity and mortality upon super-infection. Vaccine responses in this setting are often blunted, with poor response rates to HBV vaccinations in chronically HCV-infected individuals compared to healthy subjects. In this study, we investigated the role of T cell immunoglobulin mucin domain-3 (Tim-3)-mediated immune regulation in HBV vaccine responses during HCV infection. We found that Tim-3, a marker for T cell exhaustion, was over-expressed on monocytes, leading to a differential regulation of IL-12/IL-23 production which in turn TH17 cell accumulation, in HCV-infected HBV vaccine non-responders compared to HCV-infected HBV vaccine responders or healthy subjects (HS). Importantly, ex vivo blockade of Tim-3 signaling corrected the imbalance of IL-12/IL-23 as well as the IL-17 bias observed in HBV vaccine non-responders during HCV infection. These results suggest that Tim-3-mediated dysregulation of innate to adaptive immune responses is involved in HBV vaccine failure in individuals with chronic HCV infection, raising the possibility that blocking this negative signaling pathway might improve the success rate of HBV immunization in the setting of chronic viral infection.