Sabe-se que TRAIL é capaz de matar células tumorais de forma seletiva e que TRAIL tem sua expressão reduzida em diversos tumores, porém pouco se sabe sobre os mecanismos responsáveis pela sua inibição. Tendo em vista que a expressão de TRAIL pode ser regulada pelo Ácido Retinóico; que PRAME é capaz de inibir a via do ácido retinóico através da proteína EZH2 e que nós observamos anteriormente que a expressão de TRAIL esta diminuída em pacientes com LMC, nós decidimos investigar a associação entre PRAME, EZH2 e TRAIL na LMC. Nós demonstramos que PRAME, mas não EZH2, tem sua expressão aumentada em células BCR-ABL+ e sua expressão está associada com a progressão da LMC. Alem disto, existe uma correlação positiva entre PRAME e BCR-ABL e negativa entre PRAME e TRAIL nestes pacientes. A inibição da expressão de PRAME ou EZH2 por RNAi induziu um aumento da expressão de TRAIL. Estes dados revelam um novo mecanismo de regulação responsável por diminuir a expressão de TRAIL, e geram novos possíveis alvos para a terapia da LMC e, possivelmente, também para outros tumores. / TRAIL was shown to selectively kill tumor cells. Not surprisingly, TRAIL is down-regulated in a variety of tumor cells, but the mechanism responsible for TRAIL inhibition remains elusive. Because TRAIL can be regulate by retinoic acid; PRAME was shown to inhibit transcription of retinoic acid receptor target genes through the polycomb protein EZH2; and we have found that TRAIL is inversely correlated with BCR-ABL in CML patients, we decided to investigate the association of PRAME, EZH2 and TRAIL in BCR-ABL-positive leukemia. Here, we demonstrate that PRAME, but not EZH2, is up-regulated in BCR-ABL cells and is associated with the progression of disease in CML patients. In addition, PRAME expression is positively correlated with BCR-ABL and negatively with TRAIL in these patients. Importantly, knocking down of PRAME or EZH2 by RNA interference restores TRAIL expression. Our data reveal a novel regulatory mechanism responsible for lowering TRAIL expression and provide the basis of alternative targets for combined therapeutic strategies for CML.

Bien que constitué anatomiquement et physiologiquement pour la course d’endurance, l’Homme est considérablement exposés à diverses blessures musculo-squelettiques liées à la répétition de contraintes mécaniques. Le coureur de trail running par exemple est soumis à de nombreux impacts ainsi qu’à une fatigue et des dommages musculaires sévères. Ces chocs répétitifs et dommages musculaires réduiraient la tolérance du coureur face aux contraintes mécaniques le poussant ainsi à altérer sa cinématique de course. Par conséquent, minimiser les dommages musculo-squelettiques serait déterminant pour la performance en trail running. Des évidences montrent que la pose de pied altère la localisation et l’intensité des contraintes appliquées au système musculo-squelettique. L’objectif de ce travail de thèse a été d’étudier l’influence du pattern de pose de pied sur l’impact et la fatigue neuromusculaire en trail running. Les phases de descente ont été tout particulièrement étudiées du fait qu’elles sont les plus traumatisantes. En effet, ce travail de thèse a mis en évidence qu’en situation de trail running, l’intensité de l’impact augmente lorsque la pente diminue, et que la fatigue neuromusculaire périphérique est aussi sévère à la suite d’une descente isolée qu’après un ultra-trail de plusieurs heures. En étudiant l’influence de la pose de pied adoptée au cours d’une descente en situation de trail running, il a été observé qu’attaquer le sol par l’avant du pied augmentait la fatigue neuromusculaire aux extenseurs du genou. Cependant, une importante variabilité dans les patterns de pose de pied adoptés au cours de la descente a été associée à une baisse de la fatigue neuromusculaire aux extenseurs du genou et fléchisseurs plantaires. L’influence de la pose de pied sur l’intensité du choc et le contenu vibratoire le long des axes axial et transversal a également été démontrée : adopter une attaque talon diminue la sévérité du choc axial mais réduit l’intensité du choc transversal. La principale conclusion est qu’aucun pattern de course ne saurait être universellement recommandé du fait que « changer de pose de pied » est synonyme de « changer la localisation et la magnitude des contraintes appliquées au système musculo-squelettique ». En ce sens, alterner entre différents patterns de course serait une stratégie efficiente en trail running / Although Humans are “born” anatomically and physiologically adapted to long distances run, they are substantially exposed to various musculoskeletal overuse injuries. Trail runners sustain a high number of foot-to-ground contacts and develop severe muscle fatigue and damages. Repetitive shocks and muscle damages would reduce the runners’ tolerance to mechanical strains leading to changes in running kinematics. Minimizing musculoskeletal damages is therefore considered paramount for performance in trail running. Numerous studies highlighted that the foot strike pattern alters the localization and magnitude of the mechanical strains applied on the musculoskeletal system. The main purpose of this thesis was to study the influence of the foot strike pattern on impact and neuromuscular fatigue in trail running. Downhill sections were mainly investigated since they are the most mechanically stressful. Indeed, it was observed from this thesis’ work that, in real trail running practice, the impact intensity increases as the slope decreases, and that the neuromuscular fatigue induced by a single downhill run is as severe as the one induced by an ultratrail race that lasts several hours. Investigating the effect of the foot strike pattern adopted during a downhill trail run on fatigue, it was observed that forefoot striking increases the neuromuscular fatigue at knee extensors. However, a high variability in foot strike patterns adopted was associated to a lower neuromuscular fatigue at both knee extensors and plantar flexors. The effect of the foot strike pattern on axial and transversal shock and vibration content was also demonstrated: heel striking was correlated to a lower impact severity along the axial axis of the skeleton but a greater one along its transversal axis. The main conclusion of this thesis is that no single foot strike pattern should be universally advised due to “changing of foot strike” means “changing the localization and magnitude of the mechanical stress applied on the musculoskeletal system”. Switching between different running patterns might be an efficient strategy in trail running

The diploma thesis deals with treatment of project documentation of the corridor for pedestrians and bicyclists on road Blansko ČKD – Nový Hrad. It is a common two-way pedestrian and bicycle trail along the Svitava River and railway line No. 260 Brno - Česká Třebová and the regional road II / 379. Part of the design will be any footbridges across the Svitava River and other construction solutions to overcome difficult terrain in the problematic section. Part of the diploma thesis is the concept of a variant directional solution of the route and justification of the selection of a more detailed variant.

Title: Natural and Historical view of the area Dolní Poohří between Libochovice and Budyně nad Ohří. Abstract: This presented thesis deals with detailed characteristics of Dolní Poohří in the area between Libochovice and Budyně nad Ohří. The theoretical part presents a general research of the area from the natural scientific and historical points of view. It outlines geological and pedological situation of the area which influences plant composition. Majority of the thesis is dedicated to the ecosystem of bottomland woods and to their historical evolution in the area of Dolní Poohří. It focuses on the flora and dendroflora of the observed locality and links them to mutual connection. It also summarizes individual local species of vegetation. This thesis also describes the history of the area and points out especially castle area Libochovice and Budyně nad Ohří from the historical point of view. It offers a brief historical overview of modifications of castle garden and park, as well as their current situation and plant composition. In the practical part, an educational tourist trail from Libochovice to Budyně nad Ohří has been designed. Route description, graphics and contents of the information panels, as well as work-lists for elementary and high school students are parts of the project design. An...

This diploma thesis has aimed to elaborate a nature trail in the area of Klánovice Forest, which is the part of a nature park Klánovice - Čihadla in the eastern part of Prague and to complete the trail with electronic educational materials. The nature trail itself will consist of tagg stops enabling visitors to download texts and materials into their cell phones or tablets. The texts of the stops are supplemented with educational presentations, school work sheets and field work sheets. The content of the stops and related teaching materials have been prepared in accordance with systematic teaching of natural history and biology at primary schools and lower grades of grammar schools. The thesis also contains the results of a questionnaire survey of pupils' attitudes towards the use of modern technology at class; 213 questionnaires have been evaluated in total in the years 2008, 2009, 2011 and 2013. The last part of the thesis and appendices contain own texts of stops and related educational materials. In spring 2014 this trail will be realized and educational materials will become a part of the texts on the individual stops and will be available to wide public. Key words: Klánovice Forest, field trip, nature trail, electronic educational materials

Master of Landscape Architecture / Department of Landscape Architecture, Regional and Community Planning / Jason Brody / The Mid America Regional Council, (MARC) is the official metropolitan planning organization for the Greater Kansas City region. MARC received a 4.25 million dollar grant from the US Department of Housing and Urban Development in 2010 to advance the region’s vision of sustainability through vibrant, green, and connected centers and corridors. From which the CSP (Creating Sustainable Places) initiative was established. Responding to this initiative, eight grant funded Kansas State University students worked in a Rock Island Corridor group. This group showed and described ways of achieving MARC’s and the US Department of Housing and Urban Development’s goals through the development of Master’s Reports. Reports include design ideas and proposals which MARC and city planning departments might not have developed otherwise. Student collaboration between public and city planning departments informed the development of student ideas. Green trail systems and tourism specifically addresses how the quality of life in the Kansas City Metro Area could be enhanced with the implementation of a cultural trail network. This network would provide access to the Rock Island Corridor, create greenspaces, and establish mixed-use housing districts for potential home and business owners. The Greater Kansas City region population is increasing. Trails, parks, and mixed-use buildings for residents in KC could improve the quality of life for future residents. Adding greenspaces, pedestrian mobility, and entertainment districts in which to find region specific activities could help attract people to redeveloping areas. Green trail systems and tourism’s project area is located between the Sprint Center and the Truman Sports Complex. To resolve problems and dilemmas within this region of Kansas City, a routing plan of a cultural trail network is outlined. This cultural trail features new trail heads, cultural centers, and proposed zoning overlays for park developments and mixed-use housing districts. The trail network created connects the Sprint Center and Truman Sports Complex in Kansas City, Missouri as a tourism link. Tourism destinations and historic sites add to the economic success of KC. Sites are connected to in the routing plan for the cultural trail. This proposal shows one approach for creating sustainable centers in KC.

As áreas protegidas são importantes espaços de conservação da natureza e de práticas turísticas e recreativas. A categoria de parques nacionais apresenta uma oferta natural, principalmente de trilhas para caminhadas, que proporciona ao visitante distintas experiências, porém nem sempre são garantidas qualidade e segurança como elementos inerentes à visitação turística. As trilhas abertas à visitação precisam apresentar sinalização e orientações mínimas, por meio de placas, folhetos, mapas e demais materiais informativos, para contribuir com a qualidade da experiência do visitante, ou seja, as trilhas devem ser planejadas, administradas e manejadas de maneira adequada. Neste contexto, esse trabalho discute um dos elementos essenciais da visitação em trilhas que é a necessidade de existir um sistema de classificação quanto ao grau de dificuldade, como parte integrante das ações de manejo da visitação turística, garantindo informações e contribuindo para a segurança do visitante. O principal objetivo do trabalho é elaborar um modelo para classificar trilhas de caminhada quanto ao grau de dificuldade, com o uso de geotecnologias. Para tanto, a área de estudo nesta pesquisa foi o Parque Nacional do Itatiaia (PNI), localizado na divisa entre Rio de Janeiro e Minas Gerais. Foram selecionadas para análise três trilhas localizadas na Parte Alta do parque, região conhecida como Planalto, quais sejam: Agulhas Negras, Prateleiras e Couto Prateleiras. O estudo apresenta uma abordagem qualitativa, com caminho metodológico delineado a partir de três etapas principais: 1) pesquisa bibliográfica e documental, e entrevistas com gestores do PNI, o que permitiu a seleção dos critérios utilizados para a análise do grau de dificuldade dessas trilhas: declividade, condições do terreno, cobertura vegetal e drenagem; 2) levantamento de dados geográficos em campo, com base nos critérios selecionados, a partir do mapeamento das trilhas com uso de receptor de Sistema Global de Posicionamento (GPS); e 3) aplicação de um modelo de classificação, com uso de técnicas de sensoriamento remoto, operações de álgebra de mapas e ferramentas de análises espaciais em um Sistema de Informações Geográficas (SIG), para produzir os mapas temáticos de acordo com o grau de dificuldade das trilhas. Na revisão de literatura foram identificados poucos estudos no Brasil; e ao mesmo tempo no mundo há diversos sistemas de classificação de trilhas, porém na maioria dos casos não há explicação clara sobre o método adotado, bem como não há referência quanto ao uso de geotecnologias na análise de dados; destaca-se o caso da Austrália por possuir um sistema nacional de classificação de trilhas bem estruturado. Por fim, identificou-se na pesquisa que a realização de análises espaciais deve ser considerada um dos principais alicerces do planejamento e gestão de recursos naturais e do turismo em áreas protegidas, sendo que os mapas servem tanto de apoio aos visitantes quanto para o manejo dos parques / Protected areas are important zones of nature conservation and tourism and recreational practices. The category of national parks has a natural supply mainly of hiking trails that provide the visitor with different experiences; however, quality and safety are not always guaranteed as inherent elements in tourist visitation. Trails open to visitors must present minimum signaling and guidelines through plates, brochures, maps and other informative materials, to contribute to the quality of the visitor experience, i.e., trails must be planned, managed and handled properly. In this context, one of the essential elements of visitation trails is discussed in this study, which is the need of a classification system for trails by the degree of difficulty, as part of the management activities of tourist visits, providing information and contributing to the safety of visitors. The main objective of this study is to develop a model to rank hiking trails by the degree of difficulty with the use of geotechnologies. Therefore, the area of study in this research was the Itatiaia National Park (PNI), located on the border between Rio de Janeiro and Minas Gerais. Three trails located on the upper zone of the park, region known as Plateau, were selected for analysis, which were: Agulhas Negras, Prateleiras and Couto Prateleiras. The study presents a qualitative approach, with a methodological path outlined from three main steps: 1) bibliographical and documentary research, and interviews with PNI managers, which allowed the selection of the criteria for analyzing the degree of difficulty of these trails: slope, terrain conditions, vegetation cover and drainage; 2) field spatial data collection, based on selected criteria, from mapping the trails with a Global Positioning System (GPS); and 3) application of a classification model, using remote sensing techniques, map algebra and spatial analysis tools in a Geographic Information System (GIS), to produce thematic maps according to the degree of difficulty of trails. In the literature review, it were identified few studies in Brazil; and at the same time in the world, there are several trails classification system, but in most cases there is no clear explanation of the method adopted, and there is no reference regarding the use of geotechnologies in data analysis. It is highlighted the case of Australia for having a well-structured national classification system of trails. In conclusion, it was identified in the research that performing spatial analysis should be considered one of the main pillars for planning and management natural resources and tourism in protected areas as maps serve both to support the visitors as well as for parks management

É crescente a necessidade de prover segurança às informações trocadas nos mais diversos tipos de redes. No entanto, redes amplamente dependentes de dispositivos com recursos limitados (como sensores, tokens e smart cards) apresentam um desafio importante: a reduzida disponibilidade de memória, capacidade de processamento e (principalmente) energia dos mesmos dificulta a utilização de alguns dos principais algoritmos criptográficos considerados seguros atualmente. É neste contexto que se insere o presente documento, que não apenas apresenta uma pesquisa envolvendo projeto e análise de algoritmos criptográficos, mas também descreve um novo algoritmo simétrico denominado CURUPIRA. Esta cifra de bloco baseia-se na metodologia conhecida como Estratégia de Trilha Larga e foi projetada especialmente para ambientes onde existe escassez de recursos. O CURUPIRA possui estrutura involutiva, o que significa que os processos de encriptação e decriptação diferem apenas na seqüência da geração de chaves, dispensando a necessidade de algoritmos distintos para cada uma destas operações. Além disto, são propostas duas formas diferentes para seu algoritmo de geração de chaves, cada qual mais focada em segurança ou em desempenho. Entretanto, ambas as formas caracterizam-se pela possibilidade de computação das sub-chaves de round no momento de sua utilização, em qualquer ordem, garantindo uma operação com reduzido uso de memória RAM. / The need for security is a great concern in any modern network. However, networks that are highly dependent of constrained devices (such as sensors, tokens and smart cards) impose a difficult challenge: their reduced availability of memory, processing power and (more importantly) energy hinders the deployment of many important cryptographic algorithms known to be secure. In this context, this document not only presents the research involving the design and analysis of cryptographic algorithms, but also proposes a new symmetric block cipher named CURUPIRA. The CURUPIRA follows the methodology known as theWide Trail Strategy and was specially developed having constrained platforms in mind. It displays an involutional structure, which means that the encryption and decryption processes differ only in the key schedule and, thus, there is no need to implement them separately. Also, two distinct scheduling algorithms are proposed, whose main focus are either on tight security or improved performance. In spite of this difference, both of them allow the keys to be computed on-the-fly, in any desired order, assuring a reduced consumption of RAM memory during their operation.

A indagação central que norteou esta investigação, conduzida no município de São José do Rio Pardo (SP), refere-se à incorporação de abordagens participativas na construção de estratégias educativas contextualizadas e significativas para a comunidade envolvida, criando oportunidades para reflexão, ação e disseminação de idéias e práticas conservacionistas. Assim, esta pesquisa objetivou planejar um processo participativo de desenvolvimento de uma trilha interpretativa como instrumento de educação ambiental, e analisar como a aplicação de metodologias participativas contribui para um maior envolvimento dos participantes nas diversas etapas deste processo. Representantes das secretarias municipais de Educação, de Cultura, de Turismo e de Agricultura, assim como organizações não governamentais, empresas e estudantes participaram da pesquisa. Todos envolveram-se, em maior ou menor grau, nas várias etapas de construção da trilha interpretativa: no diagnóstico prévio e escolha do local e do público-alvo; na elaboração do roteiro interpretativo; na realização e avaliação das atividades de visita à trilha por estudantes de ensino fundamental; e na avaliação do processo como um todo. As técnicas utilizadas para coleta de dados no diagnóstico e na construção da trilha foram o diagnóstico rural participativo e os grupos focais, já que com ambas as técnicas é possível lidar com a dimensão interativa de um grupo. A trilha interpretativa tem sido considerada como uma estratégia educativa capaz de transcender os aspectos cognitivos da aprendizagem, proporcionando oportunidades de desenvolvimento dos aspectos afetivos e habilidades dos educandos, podendo, portanto, ser considerada um instrumento efetivo de educação ambiental. Contudo, ela deve ser planejada e considerada como parte de um processo mais amplo e, não, apenas como um evento educativo pontual. A construção da trilha constituiu-se em uma oportunidade de reflexão individual e coletiva a respeito de temas ambientais relevantes. A metodologia participativa possibilita lidar com diferentes níveis de convívio em grupo, como o respeito pelas diferenças, a capacidade de negociação e a tomada de decisões em conjunto. O interesse inicial pelo tema, as afinidades pessoais e a experiência prévia de trabalho em grupo por parte de alguns dos participantes facilitaram o envolvimento em todas as etapas do projeto. Algumas das dificuldades que podem ser encontradas na continuidade de projetos participativos vão desde a seqüência das atividades - quando a pesquisadora se afasta do grupo - até mudanças no cenário político nos quais estes projetos tenham sido iniciados, o que justifica a importância da participação de representantes também da iniciativa privada e de ONGs. A metodologia participativa, além de gerar uma autonomia dos integrantes do grupo, proporciona maiores possibilidades de continuidade do projeto e possibilita novas iniciativas de ações de conservação e educação ambiental por parte dos envolvidos / The central issue of this investigation, which was conducted in the city of São José do Rio Pardo (SP), is the incorporation of participatory approaches to developing contextualized and significant educational strategies for the community involved, thus creating opportunities for reflection, action and spread of conservationist ideas and practices. This research, therefore, aimed to plan a participatory process of developing an interpretive trail as a tool for environmental education and assess the application of such methodologies in the engagement of the local participants in the different steps of this process. Representatives from the municipal departments for Education, Culture, Tourism and Agriculture as well as representatives of non-governmental organizations, companies and students participated in the research. All of them, to a greater or lesser extent, took part in the different steps in the process of creating the interpretive trail: the early diagnosis of the place; the choice of the place and target public; the creation of the interpretive itinerary; the accomplishment and assessment of the activities involved in the visit to the trail made by secondary school students; and the assessment of the process as a whole. Since techniques of participatory rural appraisal and focus groups can provide an interactive dimension of a group, they were used for collecting data from the diagnosis and the development of the trail. Interpretive trail has been shown to be an educational strategy capable of transcending the cognitive aspects of the learning and providing students with opportunities to develop their affective aspects and skills. Thus, it can be considered as an effective tool for environmental education. Nevertheless, it should be first considered as a part of a wider process rather than a prompt educational event. The process consisted of opportunities for individual and collective reflection on the relevant environmental themes. The group life, the respect for differences, and the ability to compromise and make collective decisions were evolved. The initial interest in the theme, the personal affinities, and the previous experience some of the participants had in working group facilitated the engagement, participation and articulation. Some of the difficulties in continuing participatory projects range from the sequence of activities – when the researcher leaves the group – to changes in the political context in which they were initiated. It can explain the importance of the participation of representatives from the private and service sectors. The participatory methodology generates the autonomy of the participants, thus promoting further possibilities for continuing the project and allowing new initiatives for conservationist actions and environmental education from the participants

Grande parte das ameaças à biodiversidade dos ambientes marinhos e costeiros é resultado direto da população humana e das tendências de aumento demográfico. Uma das dificuldades para a proteção dos ambientes naturais está na existência de diferenças nas percepções dos valores e importância dos mesmos entre os indivíduos de culturas diferentes ou de diferentes grupos. Consideramos que uma importante etapa para a conservação da biodiversidade é entender a Percepção Ambiental dos sujeitos que compõe a população local. Entendemos como Percepção Ambiental a relação que indivíduo estabelece com o meio no qual está inserido, que ocorre através de mecanismos perceptivos e cognitivos. O presente trabalho visa contribuir na ampliação do conhecimento de tal temática, principalmente enfocando Ambientes Marinhos e Costeiros. Para isso, investigamos a Percepção Ambiental de um grupo de professores de uma escola pública do litoral norte de São Paulo (Ubatuba, SP) que participaram de uma vivência formativa em atividades de Educação Ambiental. Este trabalho apresenta como objetivos específicos: 1) verificar se e como a participação nas atividades educacionais influenciou a Percepção Ambiental de tais professores; 2) investigar as concepções que os professores apresentam sobre as possibilidades e desafios da prática docente ao abordar o ambiente marinho e costeiro a partir dos subsídios fornecidos pela vivência formativa. Os professores participaram das atividades do Projeto Trilha Subaquática que foram desenvolvidas ao longo de dois dias no Parque Estadual da Serra do Mar e no Parque Estadual Ilha Anchieta, ambos em Ubatuba, São Paulo. Utilizamos instrumentos de coleta de dados que foram aplicados tanto no início quanto no final da vivência (questionário e redação), além do registro audiovisual referente às Reflexões Coletivas realizadas ao final de cada dia. A partir da análise dos dados coletados, que foi feita por categorização aberta e por análise de conteúdo categorial, foi possível notar que participar das atividades de Educação Ambiental influenciou, em alguns aspectos a Percepção Ambiental dos professores. Tal influência ficou mais evidente em aspectos específicos, como, por exemplo, em relação à biodiversidade destes ambientes. Ainda que a percepção dos professores em relação a causarem impactos ao ambiente durante a realização das atividades não tenha sido expressa por todos, quando tais impactos foram percebidos, estavam bastante relacionados aos organismos do costão rochoso, especificamente ao pisoteio dos mesmos. Verificamos ainda aspectos interessantes sobre as representações sociais desses sujeitos que, apesar da participação na vivência, continua sendo majoritariamente antropocêntrica. Sobre as possibilidades e desafios da prática docente ao abordar os ambientes marinhos e costeiros, pudemos notar que para os professores sujeitos desta pesquisa, as atividades de campo são as mais significativas para se trabalhar tal tema. Além disso, pudemos notar a grande importância que a troca de experiências entre os pares, como os proporcionados pela vivência, apresentam na composição das práticas docentes. / Much of the threats to marine and coastal biodiversity are directly result of human population and tendency of demographic increase. One of the difficulties to protect natural environments relies in the differences in perceptions of the values and importance of those between individuals of different cultures or different groups. We consider that understand Environmental Perception from local population is an important step for biodiversity conservation. We understand as Environmental Perception the relation that people establish with the environment in which it is inserted, that occurs through perceptual and cognitive mechanisms. The aim of this work is contribute to expansion of the knowledge about the concept, mainly focusing in Marine Environment e Coastal Environment. For that, we investigated the Environmental Perception of a group of teachers from a public school in north coast of São Paulo State (Ubatuba) who participated in a formative experience in Environmental Education activities. This work presents as specifics goals: 1) verify whether and how the participation in educational activities influenced the environmental perception of such teachers; 2) investigate the conceptions that teachers have about the possibilities and challenges of teaching practice in addressing marine and coastal environment from the subsidies provided by the training experience. The teachers participated of Underwater Trail Project activities that are developed along two days in Serra do Mar State Park and in Ilha Anchieta State Park, both in Ubatuba, São Paulo State. We use data collection instruments which were applied at the beginning and at the end of the experience (a questionnaire and a composition), besides the transcripts of the audiovisual material related to Collective Reflections made at the end of each day. From the analysis of the data collected, it was done by open categorization and categorical content analysis, it was noticeable that participate in environmental education activities influenced, even subtly, teacher\'s Environmental Perception. This effect was more evident in specific aspects, such as, for example, in relation to biodiversity of these environments. Although the perception of teachers in relation to cause impacts on the environment during the course of the activities it has not been expressed by all, when such impacts were perceived, were closely related to the rocky shore organisms, specifically to trampling them. We also found interesting aspects about the social representations of those guys who, despite participating in the experience, remains largely anthropocentric. About the possibilities and challenges of teaching practice in addressing marine and coastal environments, we noticed that for subject teachers of this research, field activities are the most significant to work with such a theme. In addition, we noted the great importance that the exchange of experiences among peers, as provided by the experience, present in the composition of teaching practices.