Physically active females, or female athletes, are at risk for the condition knownas the female athlete triad (Triad). The Triad is made up of three components that are distinctly separate, but intertwined: consuming inadequate energy for how much they are exercising, poor bone health, and problems with their menstrual periods. When female athletes don’t consume enough energy for how much they exercise, there can be hormonal imbalances, causing the body to conserve energy from some important functions including menstruation, and increased bone turnover resulting in a higher risk of stress fractures and early osteoporosis. Female athletes at risk for the Triad have ahigher risk of being injured, and don’t have the energy that they need to perform physically. To combat the Triad, we performed assessments to see how many female athletes were at risk for the Triad in Division I female athletes, and were surprised at the high number that were at risk for the Triad. We provided nutrition information and interventions aimed to decrease female athlete’s risk for the Triad. We also developed anew, quick method for female athletes, or busy individuals, to track their diet using the video feature on their cell phones. The benefits of providing nutrition information to the female athletes included: increasing their knowledge about the Triad, and things that they could do to prevent the Triad. The interventions did cause behavior change in some female athletes to increase how much energy they were consuming, decreasing their risk for the Triad.

Syftet med denna uppsats är att undersöka hur samspelet mellan föräldrar avspeglas i samspelet mellan föräldrar och barn, utifrån ett intersubjektivt perspektiv. Uppsatsen är genomförd som en pilotstudie, med ett begränsat urval, för att pröva tillämpningen av intersubjektivitet som utgångspunkt för metod och tillämpning i samspelsstudier. Fem familjer har ingått i undersökningen och filmer av deras samspel har kodats och analyserats. Uppsatsen ger intressanta utgångspunkter för fortsatt forskning på området. En slutsats som dras är att samspelet avspeglas genom att hög grad av delad uppmärksamhet, intentionalitet och synkronicitet i föräldrardyaden har starkt samband med detsamma i föräldra/barn-triaden, vilket kan anses vara användbar kunskap inom familjerådgivande och föräldrastödjande verksamheter.

The subsurface environmental contamination investigation, assessment and remediation of petro-chemical contaminated site have been the most challenging environmental issues of environmental protection. If subsurface environmental mediums like soil and groundwater are contaminated, the investigation and remediation will always be time-consuming and costly even though they could not be cleaned up completely. Groundwater contaminated by chlorinated volatile solvents is more difficult to remedy owing to its property of dense non-aqueous phase liquid (DNAPL). This study focuses on petro-chemicals contaminated site whose subsurface environment is contaminated by chlorinated ethene. The study will also find out the efficient, effective and economic ways to identify and concretely delineate the characterization of subsurface environment and contamination, conduct human health risk management and take risk-based corrective action, so as to formulate an appropriate risk management strategy and solution. Firstly, this study applies a Triad approach to accelerate and streamline the investigation process of contaminated site. Introduction of project systematic planning, dynamic work strategy and in situ, real time survey or direct-push sampling like membrane interface probe, together with off-site measures like physical measurement and chemical analytic testing in laboratory, could reduce the key uncertain characteristics of subsurface environmental contamination and establish the conceptual cite models for health risk assessment cost-effectively. It is estimated that this method could save 6 million NTD and shorten 3 years¡¦ time in site characteristics delineation and remediation. If we wish to make the site defensible via managing the uncertain decision, the integration of Triad approach which is developed by United States Environmental Protection Agency, and Environmental Site Assessment, Accelerated Site Characterization, Health Risk Assessment and Risk-based Corrective action which were developed by American Society for Testing and Materials (ASTM), are proved in this study that they could help streamline the site characterization and remediation. This study is based on the conceptual site models of Triad Approach, the Risk-based corrective action of ASTM, the Health Risk Assessment and Evaluation Methods for Soil and Groundwater Contaminated Site, and also the software developed by the Taiwan Environmental Protection Administration. Through the health risk assessment of chlorinated ethenes contaminated site, this research aims to evaluate the quantitative health risk like chemical of concern, environmental medium, and human exposure pathway etc. The result of evaluation showed that the carcinogenic risk of tier 2 is 1.080E-01 which exceeds the acceptable risk-based standard, 1E-06; tier 2¡¦s non-carcinogenic risk, 1.819E+03, also exceeds the acceptable hazard index which is 1. Groundwater poses the highest risk among environmental media, followed by inhalation of vapor from contaminated soil and/or groundwater. The risk of inhalation of top soil particulates is the lowest. The most dangerous carcinogenic exposure of groundwater is using groundwater for shower and washing whose risk is 8.064E-02; the second highest carcinogenic pathway is groundwater ingestion. Trichloroethylene is the key chemical of concern which poses 95.6% of carcinogenic risk among all chemicals of concern. Groundwater ingestion poses the highest non-carcinogenic risk (8.919E+02), and the main chemical of concern is cis-1, 2-dichloroethylene. The main chemical of concern of soil volatilization is cis-1, 2-dichloroethylene, followed by trichloroethylene. If the site in this study wishes to reduce the health risk of human beings, it is suggested to simultaneously implement integrated risk-based corrective actions including institutional control, engineering control and remediation. For institutional control, prohibition of pumping groundwater and pumping well installation is the most cost-effective choice. For engineering control, concrete capping on uncovered surface soil is feasible when dealing with soil contamination. When it comes to groundwater contamination, steel sheet pile containment or pumping well installation could help intercept the migration of groundwater. Regarding remediation, in-situ chemical reduction injection or permeable reactive barrier, incorporated together with monitored natural attenuation are more practicable alternatives, however, they are time-consuming and costly. The process and result of this study could be the basis for managers and/or decision makers of contaminated site to conduct risk-based management and decision making.

Background: The School Health Program (SHP) in the Republic of Panama includes preventive healthcare services delivery and referral issuing at elementary schools nationwide. Despite these early prevention efforts, a majority of referrals are apparently not achieving their desired outcome. This idea is supported by the SHP data showing preventable diseases on the rise. Thus, learning the outcome rates of particular referral outcomes may provide a basis for appropriately targeted action. Methods: Three focus groups were conducted with health workers, medical records staff, and teachers, respectively. Following systems thinking and framework analysis, a pilot plan for referral tracking and referral outcome rates obtainment was developed. Finally, the SHP team was surveyed for their perception on the effectiveness and feasibility of the plan, for future implementation. Results: Themes related to referral tracking led directly to the development of a referral tracking pilot plan (RTPP). Survey data analysis revealed that the SHP team perceived the RTPP as an effective way to obtain complete referral tracking and referral outcome rates, and they also found it feasible to implement. Conclusion: Keeping referral records and tracking the SHP referrals is perceived, by those that will be involved in its delivery, as achievable by implementing a RTPP developed from their own recommendations. Once implemented, the resulting obtainment of referral outcome rates may allow them to know if the SHP preventive objective for issuing these referrals is being properly achieved, and to prioritize for targeted action where needed.

Aromatic stacked salt bridges are increasingly observed to play an important role in biology, suggesting that the two separate weak interactions cooperate with each other to mediate molecular recognition in a biological solution. In this thesis an in depth study was carried out in attempt to find the contribution of the guanidinium-carboxylate-aromatic triad in biological systems. Two different small molecule systems are used to carry out the study. From the results of the two chapters I proposed here that stacking aromatic ring enhances the salt bridge through desolvation effect. This hypothesis was also tested in a protein-protein interaction (Grb2 SH3 domain/SOS interaction). The most ideal peptide inhibitor cannot be obtained due to the synthetic difficulties. Limited result showed that increasing the hydrophobic area of the hot spot in this protein-protein interaction enhances the interaction. In researching the guanidinium-carboxylate-aromatic triad, we were inspired to study the pre-organization effect of 1,3,5-triethyl-2,4,6-trisubstituted benzene template. A computational and literature study done in this thesis showed that the installation of ethyl or methyl groups at 1,3,5 positions leads to consistent increases in binding affinity relative to unsubstituted hosts, but the amount of increase is non-trivial and varies with different substitutes. The installation of ethyl or methyl groups at 1,3,5 positions leads to consistent but relatively small increases in binding affinity relative to unsubstituted hosts. / Graduate

La contraction musculaire est provoquée par un relâchement massif de calcium à partir du reticulum sarcoplasmique (RS) des cellules musculaires. Ce relâchement de calcium réalisé par le récepteur de la ryanodine (RyR1), s'effectue dans des structures membranaires spécialisées et très organisées : les triades. Cette architecture spécifique est essentielle à l'activité correcte de RyR1. Cependant, les mécanismes moléculaires mis en jeu dans la formation et le maintien des triades ne sont pas connus. La triadine, qui est une protéine localisée dans la membrane du RS et qui est associée à RyR1, pourrait jouer un rôle dans la structure du reticulum sarcoplasmique pour permettre un relâchement de calcium efficace. L'équipe a montré que l'ablation du gène de la triadine chez la souris induisait une altération des relâchements de calcium et une modification de la forme des triades.Nous avons montré que la triadine pouvait indirectement interagir avec les microtubules et qu'elle pourrait ancrer le RS aux microtubules (Fourest-Lieuvin, J Cell Science, 2012). Par analyse en spectrométrie de masse des protéines co-immunoprécipitées avec la triadine, nous avons identifiéun nouveau partenaire de la triadine, CLIMP-63 qui pourrait être impliqué dans cette fonction. CLIMP-63 est décrite comme une protéine capable d'ancrer le reticulum aux microtubules et de maintenir la forme du reticulum endoplasmique. Nous avons ensuite confirmé son interaction avec la triadine par différentes approches dans différents modèles cellulaires. L'étude et la caractérisation de CLIMP-63 dans le muscle sont tout à fait innovantes et nous avons étudié les conséquences de l'association triadine/CLIMP-63 pour la fonction du muscle et dans la formation ou la maintenance des triades. / Muscle contraction is achieved when an efficient excitation signal at the plasma membrane triggers intracellular calcium release. This process called “excitation-contraction (E-C) coupling” relies on a macromolecular protein complex, spanning the plasma membrane and the sarcoplasmic reticulum (SR), containing the calcium channel of the SR, the ryanodine receptor (RyR1). This calcium release complex is present exclusively in highly organized membrane structures called triads. A triad is composed of two SR terminal cisternae surrounding a plasma membrane transverse-tubule.This architecture is essential to sustain the activity of the calcium channel RyR1, which is located in the membrane of SR terminal cisternae. However, little is known about the molecular mechanisms allowing the formation and maintenance of SR terminal cisternae. Triadin is a member of this complex, present in the SR membrane and interacting with RyR1. Deletion of the triadin gene leads to partial disorganisation of SR membranes in skeletal muscles, with abnormal orientation of part of the triads. Triadin could play a role in the structure of sarcoplasmic reticulum to allow efficient E-C coupling. We have shown that triadin could indirectly interact with the microtubules, and therefore anchor the sarcoplasmic reticulum to the microtubule network (Fourest-Lieuvin, J Cell Science, 2012). Using mass spectrometry analysis of proteins co-immunoprecipitated with triadin, we have identified a new partner of triadin, CLIMP-63 which could be involved in this function. CLIMP-63 is a shaping protein able to mediate the anchoring of the reticulum to microtubules and to maintain the shape of endoplasmic reticulum. We have dissected the interacting domains between CLIMP-63 and triadin, and study the consequences of this association for muscle function, and triad formation or maintenance.

The Female Athlete Triad, consisting of the interrelated conditions of low energy availability, leading to menstrual disturbances and low bone mineral density, is commonly diagnosed amongst excessively exercising women. The American College of Sports Medicine emphasizes that the underlying factor of the Triad is a discrepancy between dietary energy intake and the energy requirements needed to support high levels of physical activity in addition to other homeostatic and physiological bodily processes. Although low energy availability is largely recognized as a causative factor for amenorrhea and low bone density, no studies to date have examined specific macro- and micro-nutrient intake relating to bone mineral density in the female athlete population. The hypothesis to be tested was that a difference in the intake of specific nutrients between athletes with menstrual disturbances (amenorrheic) and regularly menstruating (eumenorrheic) athletes contributes to low bone mineral density in female athletes exhibiting symptoms of the Triad. Methods: 4-day food records were collected from 118 females, ages 14-23 years, who exhibited weight within the normal range. 68 participants were amenorrheic athletes (AA), 24 participants were eumenorrheic athletes (EUM), and 26 participants were non-exercising females within the same age group (non-athletes: NA). Serum levels of vitamin D, phosphorus, calcium, and estradiol were also collected. Results: In contrast to previous studies, there was no difference in energy availability between the AA, EUM, and NA groups. The groups did differ in their intake of several macro-and micro-nutrients, and many of these nutrients correlated significantly with lumbar spine BMD. In a multivariate model that included vegetable and total proteins, soluble, insoluble and total dietary fiber, pectins, phytic acid, natural folate, calcium intake, vitamin D intake, serum vitamin D levels, and % calories from saturated fatty acids (SFA), only dietary fiber remained negatively associated and % calories from SFA positively associated with lumbar spine BMD. Conclusions: Dietary fiber has a significant inverse association and % calories from SFA a positive association with lumbar spine BMD, even after controlling for other nutrient intake and serum levels and intake of Vitamin D and calcium. Therefore, fiber and saturated fat may exert effects unrelated to vitamin D status and overall energy availability to impact bone density. Nutrition guidelines for female athlete triad patients need to be reassessed.

Experimentos em mesocosmos, por se tratarem de estudos in-situ, possibilitam extrapolar para o campo, investigações realizadas em menor escala, com maior seguridade. Em fevereiro de 2002, foram instalados às margens da represa do Lobo (22º15\'S e 47º49\'W) nove mesocosmos, construídos com plástico (PVC) em forma de hexágono com 2 metros de altura, com fundo aberto em contato com o sedimento e volume variando entre 4,5 e 5,1 m3, em função da declividade do terreno. De acordo com a resolução 20/86 do Conselho Nacional do Meio Ambiente, as concentrações máximas permitidas para os metais cobre (0,02 mg/L) e cromo (0,5 mg/L) em corpos d\'água Classe 2 foram adicionadas aos mesocosmos, originando-se triplicatas, adotando-se outros três mesocosmos como controles. O objetivo principal deste trabalho foi verificar as possíveis alterações no ambiente sedimento, particularmente o enriquecimento e a indução de toxicidade, em função da adição dos metais mencionados. Para tanto, amostras foram coletadas através de dragas e testemunhos nos tempos 0 dia (antes da contaminação), 10 dias, 20 dias e 30 dias após a contaminação (final do experimento). Em seguida, as amostras foram preparadas e submetidas às análises físicas e químicas, caracterização da macrofauna bentônica e diversos ensaios toxicológicos. A partir dos dados gerados, efetuou-se o estudo integrativo da tríade de qualidade de sedimentos. Os resultados obtidos mostraram um discreto aumento na concentração total dos metais cobre e cromo nos sedimentos dos mesocosmos contaminados em comparação aos controles, sendo verificada diminuição na abundância de organismos bentônicos e efeitos tóxicos sobre os organismos-teste submetidos a estes sedimentos. As respostas da tríade foram essenciais para a caracterização da qualidade dos sedimentos dos mesocosmos controle em relação aos dos mesocosmos contaminados. / In-situ mesocosms experiments allow the extrapolation of laboratory data to the field with high reliability. In the present study, nine hexagonal PVC mesocosms with 2 m height and 4.5 to 5.1 m3 volume were installed in contact with the sediment near the Lobo reservoir dam (22º15\'S e 47º49\'W) in february of 2002. In accordance with the National Environmental Council (CONAMA) 20/86 resolution for Class 2 water bodies, the maximum allowed concentrations of copper (0,02 mg/L) and chromium (0,5 mg/L) were added to the mesocosms (triplicate), and three other ones were used as controls. The main purpose of this study was to evaluate the possible changes in the sediment environment, particularly the enrichment and induction of toxicity, by the contamination of these metals. The sediment was sampled by means of Eckman-Birge and core samplers on the 0 (before the contamination), 10th, 20th and 30th days (end of the experiment). Afterwards, physical and chemical analyses, benthic macrofauna characterization and several toxicity assays were conducted with the samples. In order to better evaluate these results, the sediments quality triad was considered. A discrete increase in the total concentration of copper and chromium was observed in the sediments of the contaminated mesocosms when compared to the controls, as well as a reduction in the abundance of benthic organisms and toxic effects over the organisms tested with these sediments. The triad responses were essential for quality characterization of the control mesocosms sediments in comparison with the contaminated ones.

This dissertation examines the iconographical motif of the Apollonian triad in Attic art of the sixth and fifth centuries BC. Attic vase paintings constitute the chief evidence for this study, but other evidence, such as inscriptions, literary sources, sculptures and coins is considered, as well. My thesis focus on scenes without a clear mythological context, where the triad appears alone or accompanied by other, mostly, divine figures, and on what messages or information these images of the Apollonian triad convey. This study contributes to the ongoing discussion of the iconography and iconology of Attic vases, which enriches our understanding of Athenian socio-political and religious life and of Greek culture, more generally.

Several hundred chemical analyses of early Proterozoic lavas of the Witwatersrand triad (incorporating the Dominion Group, Witwatersrand Supergroup and Ventersdorp Supergroup) in the Klerksdorp area, have revealed the presence of various distinct magma types. These essentially correspond to formally defined lithostratigraphic units, but several inconsistencies have necessitated the use of informal nomenclature. The lavas have been regionally metamorphosed to low-grade, greenschist facies assemblages. Original igneous textures are preserved, despite a metamorphic overprint. Metamorphism has resulted in a certain degree of random chemical remobilization. Ba, Sr, Rb, K₂0, Na₂0 and CaO have been highly mobile, and their usefulness in petrogenetic modelling is extremely limited. In contrast, Zr, Nb, Y, LREE's, Cr, Ni, Ti0₂ P₂0₅ and Al₂0₃ have remained immobile. Ti/Zr and Ti/P ratios together constitute efficient discriminating variables for characterizing the different magma types. Lava compositions range from primitive Mg-rich tholeiites to rhyolites, the bulk being tholeiitic andesites. Al₂0₃ contents do not exceed 15%, a feature which reflects the tholeiitic, as opposed to calcalkaline, character of these lavas. Two magma-types are present within the Dominion Group, which is a typical example of bimodal volcanism. The Dominion basic lavas are overlain by the Dominion acid porphyries, with a limited amount of interfingering. The basic lava suite is highly fractionated, with compositions ranging from Mg-, Cr- and Ni-rich tholeiites (close to primary mantle melts) to evolved tholeiitic andesites. The most primitive liquids evolved by 45% fractional crystallization of hornblende, followed by a further 70% crystallization of an orthopyroxene-plagioclase assemblage containing up to 3% sulphides. The Dominion porphyries are rhyolitic, display very limited compositional variation, and probably represent a crustal melt related to the same magmatic event which produced the basic lavas. The only lavas from the Witwatersrand Supergroup present in the Klerksdorp area are those of the Crown Formation (Jeppestown amygdaloid). These are tholeiitic dacites which display extremely limited compositional variation, and are unrelated to any of the other magmas of the Witwatersrand triad. The Ventersdorp Supergroup comprises 4 magma-types: The Kliprivierberg Group lavas at the base are subdivisible into 3 sub-types on the basis of Zr contents. (Zr>11Oppm) are the most evolved. They are tholeiitic andesites which display fairly limited compositional variation. It is likely that more evolved compositions are present in other areas where the porphyritic lavas which characterize this unit are better developed. The overlying Orkney lavas are characterized by 110ppm>Zr>90ppm. They are tholeiitic andesites of similar composition to the Alberton lavas, but have lower incompatible element levels, higher siderophile element levels, and are of extremely uniform composition. The uppermost Loraine/Edenville lavas range from magnesian tholeiites to tholeiitic andesites. They are distinguished by Zr< 90ppm, and contain the most primitive magmas af the Witwatersrand triad, with up to 17,5% MgO, 2600ppm Cr, 600ppm Ni and M-values up to 77. The most primitive liquids evolved by 38% fractional crystallization of orthopyroxene ∓ chromite, followed by 35% fractional crystallization of an extract containing clinopyroxene and plagioclase. The absence of olivine precipitation is a result of the inherently high Si0₂ content of the magma. The Loraine/Edenville, Orkney and Alberton lavas do not lie on a common liquid line of descent, but are probably consanguinous. The Platberg Group overlies the Kliprivierberg Group, and has a coarse-clastic sedimentary unit, the Kameeldoorns Formation, at the base. Three petrographically distinct porphyritic lava sequences overlie the Kameeldoorns Formation, namely the informal "Goedgenoeg formation", the Makwassie quartz-feldspar porphyries and the Rietgat Formation. Despite petrographic differences, the Goedgenoeg and Rietgat lavas are chemically indistinguishable and thus form a single magma-type. The Makwassie porphyries are dacitic in composition with a high proportion of feldspar and quartz phenocrysts. Rational variation trends are attributed to a nett loss of Si0₂ during secondary alteration. The porphyries are probably of crustal origin. The Goedgenoeg/Rietgat lavas display unusual chemistry and a broad, irrational compositional spectrum. They contain very high incompatible element levels, high nonnative quartz, as well as high MgO, M-values, Cr and Ni relative to the other tholeiitic andesites of the Witwatersrand triad. It is tentatively suggested that they are hybrid magmas containing both crust and mantle components, the former possibly represented by the Makwassie porphyries. Field evidence suggests that Platberg volcanism commenced directly after Klipriviersberg volcanism ceased, and was accompanied by a period of enhanced tectonic activity. The Platberg lavas thus probably reflect a crustal melting cycle associated with the Klipriviersberg magmatic event. The Allanridge lavas are the youngest rocks of the Witwatersrand triad. They are separated from the Platberg Group by a unit of flat-lying sediments, the Bothaville Formation, which was deposited after an extended period of peneplanation. The Allanridge lavas form a separate magma-type. They are tholeiitic andesites of similar composition to the Alberton lavas, but have higher incompatible element levels and are not consanguinous. The compositional similarities amongst the basic magma-types of the Witwatersrand triad suggests that all were generated in an hydrous mantle. Interelement ratio differences between the various magma-types nevertheless support the concept that the mantle was chemically heterogeneous during the early Proterozoic.