Global ETD Search

1	Structural examination of voltage gated potassium channels by voltage clamp fluorometry Vaid, Moninder 05 1900 (has links) Voltage clamp fluorometry (VCF) was first developed in the mid 1990s by Isacoff and his colleagues. In this approach fluorophores are attached to substituted cysteine residues that are engineered by site-directed mutagenesis. Changes in the dielectric environment of the fluorophore report local transitions that are associated with electrically-related and electrically-silent transitions. VCF provides a powerful technique to observe real time reports of ion channel gating conformations. It has proven to be a useful technique because it adds insight that is not available using other techniques. X-ray crystallography studies give a predominantly static picture of the channel, while patch clamping of channels gives information only about residues that effect ionic current flow. Similarly, gating current provides insight only about residues that are charged and move across the membrane electric field. In this thesis we examined the structural rearrangements of the Shaker channel and the effect of 4-AP on channel gating. We also examined for the first time the structural rearrangements of the Kv1.5 gating and the how the channel responds to depolarization pulses. This work is instrumental in the examination of the potassium channel gating. VCF Potassium channel gating
2	Structural examination of voltage gated potassium channels by voltage clamp fluorometry Vaid, Moninder 05 1900 (has links) Voltage clamp fluorometry (VCF) was first developed in the mid 1990s by Isacoff and his colleagues. In this approach fluorophores are attached to substituted cysteine residues that are engineered by site-directed mutagenesis. Changes in the dielectric environment of the fluorophore report local transitions that are associated with electrically-related and electrically-silent transitions. VCF provides a powerful technique to observe real time reports of ion channel gating conformations. It has proven to be a useful technique because it adds insight that is not available using other techniques. X-ray crystallography studies give a predominantly static picture of the channel, while patch clamping of channels gives information only about residues that effect ionic current flow. Similarly, gating current provides insight only about residues that are charged and move across the membrane electric field. In this thesis we examined the structural rearrangements of the Shaker channel and the effect of 4-AP on channel gating. We also examined for the first time the structural rearrangements of the Kv1.5 gating and the how the channel responds to depolarization pulses. This work is instrumental in the examination of the potassium channel gating. VCF Potassium channel gating
3	Structural examination of voltage gated potassium channels by voltage clamp fluorometry Vaid, Moninder 05 1900 (has links) Voltage clamp fluorometry (VCF) was first developed in the mid 1990s by Isacoff and his colleagues. In this approach fluorophores are attached to substituted cysteine residues that are engineered by site-directed mutagenesis. Changes in the dielectric environment of the fluorophore report local transitions that are associated with electrically-related and electrically-silent transitions. VCF provides a powerful technique to observe real time reports of ion channel gating conformations. It has proven to be a useful technique because it adds insight that is not available using other techniques. X-ray crystallography studies give a predominantly static picture of the channel, while patch clamping of channels gives information only about residues that effect ionic current flow. Similarly, gating current provides insight only about residues that are charged and move across the membrane electric field. In this thesis we examined the structural rearrangements of the Shaker channel and the effect of 4-AP on channel gating. We also examined for the first time the structural rearrangements of the Kv1.5 gating and the how the channel responds to depolarization pulses. This work is instrumental in the examination of the potassium channel gating. / Medicine, Faculty of / Cellular and Physiological Sciences, Department of / Graduate VCF Potassium channel gating
4	Structural examination of voltage gated potassium channels by voltage clamp fluorometry Vaid, Moninder 05 1900 (has links) Voltage clamp fluorometry (VCF) was first developed in the mid 1990s by Isacoff and his colleagues. In this approach fluorophores are attached to substituted cysteine residues that are engineered by site-directed mutagenesis. Changes in the dielectric environment of the fluorophore report local transitions that are associated with electrically-related and electrically-silent transitions. VCF provides a powerful technique to observe real time reports of ion channel gating conformations. It has proven to be a useful technique because it adds insight that is not available using other techniques. X-ray crystallography studies give a predominantly static picture of the channel, while patch clamping of channels gives information only about residues that effect ionic current flow. Similarly, gating current provides insight only about residues that are charged and move across the membrane electric field. In this thesis we examined the structural rearrangements of the Shaker channel and the effect of 4-AP on channel gating. We also examined for the first time the structural rearrangements of the Kv1.5 gating and the how the channel responds to depolarization pulses. This work is instrumental in the examination of the potassium channel gating. voltage clamp fluorometry VCF channel gating
5	Simulerad effektivisering av genotypdataanalys genom poolade data / Simulated optimization of genotype data analysis bypooling data Strömstedt Hallberg, Simon, Giek, Jonas January 2016 (has links) Målet med projektet är att undersöka om det går att effektivisera hur man undersöker människors gener. Detta görs genom att skapa ett program i Java. Resultatet är ett program som sorterar genotypdata från 1000 Genomes Project och utvärderar nyttan av att undersöka genotyper från flera individer samtidigt. genotype allele optimization microarray 1000genomesproject variantcontext variantcall vcf genotyp genotypdata effektivisering allele microarray 1000genomesproject minor allele frequency variantcontext variantcall vcf Computer Sciences Datavetenskap (datalogi)
6	A study of Kv channel dynamics using a fluorescent unnatural amino acid Kalstrup, Tanja 10 1900 (has links) No description available. Canaux Kv acides aminés non-naturels Anap fluorimétrie en voltage imposé Kv channels voltage clamp fluorometry VCF Unnatural amino acids UAA
7	An Investigation into the Evolution of Nucleotide Composition in the Human Genome Paudel, Rajan 06 September 2019 (has links) No description available. Bioinformatics Biology evolution human genome composition Mid-Range Inhomogeneity genomic-MRI GC AT nucleotide composition Biased gene conversion natural selection 1000 Genomes Project Simon Genome Diversity Project VCF genomic evolution
8	Demography of Birch Populations across Scandinavia Sendrowski, Janek January 2022 (has links) Boreal forests are particularly vulnerable to climate change, experiencing a much more drastic increase in temperatures and having a limited amount of more northern refugia. The trees making up these vast and important ecosystems already had to adapt previously to environmental pressures brought about by the repeated glaciations during past ice ages. Studying the patterns of adaption of these trees can thus provide valuable insights on how to mitigate future damage. This thesis presents and analyses population structure, demo- graphic history and the distribution of fitness effects (DFE) of the diploid Betula pendula and tetraploid B. pubescens across Scandinavia. Birches–being widespread in boreal forests as well as having great economical importance–constitute superb model species. The analyses of this work confirm the expectations on postglacial population expansion and diploid-tetraploid introgression. They furthermore ascertain the presence of two genetic clusters and a remarkably similar DFE for the species. This work also contributes with a transparent, reproducible and reusable pipeline which facilitates running similar analyses for related species. birch betula pubescens pendula betula pubescens betula pendula silver birch downy birch demography population structure distribution of fitness effects DFE dadi UMAP PCA ADMIXTURE polyDFE ice age glaciation tree boreal forest climate change adaption tetraploid diploid-tetraploid introgression Scandinavia genetic cluster pipeline snakemake bioinformatics reproducible population genetics postglacial population expansion demographic history FEEMS site-frequency spectrum SFS last glacial maximum LGM workflow python population expansion population growth EST-SFS 0-fold degenerate 4-fold degenerate maximum likelihood estimation MLE variant call format VCF single-nucleotide polymorphism SNP Bioinformatics (Computational Biology) Bioinformatik (beräkningsbiologi)

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