In the last decades, miniaturized flow chemistry has promised to bring the benefits of process intensification, continuous manufacturing and greener chemistry to the fine chemical industry. However, miniaturized catalytic processes where gas, liquid, and solids are involved have always been impeded by two main drawbacks: multiphase-flow maldistribution (i.e. gas channeling) and clogging of capillary reactors. In this thesis, first principle models have been used to capture the complexity of multiphase flow in micropacked-bed reactors, which can suffer from poor and unpredictable mass-transfer performance. When the particle size ranges 100 µm in diameter, capillary and viscous forces control the hydrodynamics. Under such conditions, the gas —and not the liquid— flows creating preferential channels that cause poor radial dispersion. Experimental observations from the literature were reproduced to validate a physical-based modeling approach, the Phase Field Method (PFM). This simulation strategy sheds light on the impact of the micropacked-bed geometry and wettability on the formation of preferential gas channels. Counterintuitively, to homogenize the two-phase flow hydrodynamics and reduce radial mass-transfer limitations, solvent wettability of the support needs to be restricted, showing best performance when the contact angle ranges 60° and capillary forces are still dominant. Visualization experiments showed that ultrasound irradiation can also be used to partially fluidized the bed and modify the hydrodynamics. Under sonication, residence time distributions (RTD) in micropacked-bed reactors revealed a two-order-of-magnitude reduction in dispersion, allowing for nearly plug-flow behavior at high gas and liquid flow rates. At a reduced scale, surfaces vibrating with a low amplitude were shown to fluidize, prevent and solve capillary tube blockage problems, which are commonly found in the fine chemical industry for continuous product synthesis. The modeling and simulation strategy used in this thesis, enables a fast prototyping methodology for the proper acoustic design of sonoreactors, whose scale-up was achieved by introducing slits in sonotrodes. In addition, a patent-pending helicoidal capillary sonoreactor has shown to transform longitudinal vibrating modes into radial and torsional modes, pioneering a new range of chemistry able to handle a high concentration of particles. The contributions of this thesis made in the fields of reaction engineering and process intensification have demonstrated how computational methods and experimental techniques in other areas of research can be used to foster innovation at a fast pace.

Background: “Scale-ups”, characterised by their high growth and significant contribution to economic development, receive increasing attention in today's economies. They are often young and innovative SMEs that grow rapidly across international borders. “Scale-ups” achieve growth and improved performance through an accelerated organisational development process, which is based on their knowledge resources. Purpose: The purpose of this study is to explore and theorise what constitutes a scaling capability. Specifically, we will study knowledge acquisition and integration in SMEs that scale across international markets. Method: This study has an abductive research approach that considers the current theories on scaling and the knowledge-based view on strategic management and international business. To this end, we completed a multiple-case study of 4 innovative and internationalising scale-ups to accomplish our purpose. Conclusion: The study contributes with a view of scaling as a “scaling capability” that captures how innovative scale-ups manage the internal organisation of experiential knowledge. We develop frameworks that give a generalised understanding of how scale-ups in this particular context acquire and integrate knowledge resources during episodes of growth and improved performance. These frameworks show how scale-ups start their scaling journey with a full acquisition of specialised resources in technical, business and internationalisation knowledge, which they integrate through providing direction and organisational routines to organisational members. We address the importance of having different knowledge capital in symmetry to increase the common knowledge, and we suggest that the scaling capability enables a scale-up to adapt for their changing organisational needs, which is why we liken scaling to developing organisational capability. / Contexto: Las "scale-ups", caracterizadas por su elevado crecimiento y su importante contribución al desarrollo económico, reciben cada vez más atención en las economías de hoy. Suelen ser PYMES jóvenes e innovadoras que crecen rápidamente más allá de las fronteras internacionales. Las scale-ups logran crecer y mejorar su rendimiento mediante un proceso acelerado de desarrollo organizativo, basado en sus recursos de conocimiento. Propósito: El propósito de este estudio es explorar y teorizar lo que constituya una capacidad de escalamiento empresarial. En concreto, estudiaremos la adquisición e integración de conocimientos en las PYME que escalan por los mercados internacionales. Método: Este estudio tiene un enfoque de investigación abductiva que considera las teorías actuales sobre el concepto de escalamiento empresarial, o "scaling", y la teoría de la gestión estratégica y los negocios internacionales basada en el conocimiento. Para lograr nuestro propósito, realizamos un estudio de casos múltiples de cuatro scale-ups innovadoras que se internacionalizan. Conclusiones: El estudio contribuye con un enfoque del escalamiento como "capacidad de escalamiento" que capta cómo las scale-ups innovadoras gestionan la organización interna del conocimiento experiencial. Desarrollamos modelos que ofrecen una comprensión generalizada de cómo las scale-ups en este contexto adquieren e integran recursos de conocimiento durante los episodios de crecimiento y mejora del rendimiento. Los modelos muestran cómo las scale-ups inician su proceso de crecimiento con una adquisición completa de recursos especializados en conocimientos técnicos, empresariales y de internacionalización, que integran proporcionando dirección y rutinas organizativas a los miembros de la organización. Abordamos la importancia de contar con diferentes capitales de conocimiento en simetría para aumentar el conocimiento común, y sugerimos que la capacidad de escalamiento permite a una scale-up adaptarse a sus necesidades organizativas cambiantes, razón por la cual vemos el escalamiento como el desarrollo de la capacidad organizativa.

Solvent-free extrusion emulsification (SFEE) is a recently developed process for producing submicron particles with high viscosity polymers inside a twin-screw extruder without the use of hazardous solvents. Its dependency on a catastrophic phase inversion makes the process knowingly sensitive to a variety of formulation and operational variables, causing a narrow window of production. The purpose of this thesis was to investigate and improve process stability as well as widening operational window. Transient effects of the start-up procedure was investigated by considering the process stability and particle size distribution. The transient sensitivity corresponded to the residency of material in the dispersion zone. When a sub-optimal water/surfactant fraction was allowed to produce an undesired polymer-water (thick lamella) morphology, this morphology continued to persist until the critical first half of the dispersion zone was purged of existing mass. Lot to lot variability of polyester resin was used to investigate the sensitivities of the SFEE process more deeply to better understand the mechanism involved. In this case, acid number was shown to have a significant effect on the initial amount of water needed in the dispersion zone for phase inversion, resulting in an emulsification boundary dependent on the resin acid number. In fact, a significant correlation was found between the acidic end groups of the resin and the maximum amount of water content that could be used in the dispersion zone. The effect of feed rate, screw speed, dispersion length, and surfactant concentration were studied for their individual influence on widening the emulsification boundary. The most significant improvement was observed by applying a longer dispersion length or lower feed rate because both significantly increase the residence time. The effect of residence time on the emulsification boundary was attributed to the total strain imposed on the polymer/water mixture which was related to interfacial growth in the dispersion zone. / Dissertation / Doctor of Philosophy (PhD)

No description available.

When scaling a company it is important to implement customer insights to achieve growth of revenue. Understanding and defining a suitable B2B sales process has also been shown to play an important part in enhancing sales, and traditional processes include multiple steps performed by sales representatives. One step revolves around the presentation of the offered product or service. For sales representatives to present a product or service successfully they must acquire or have deep knowledge of the customer, such as their industry trends and general business. This can be achieved by acquiring customer insights that are data driven. Adopting data driven customer insights has also been proven to increase sales. Therefore, this research investigates the connection between the B2B sales process and the generation and implementation of data driven customer insights. In particular, this research explores the steps included in a B2B sales process at a high technology scaleup and hence how data driven customer insights can enhance the presentation step in the B2B sales process.  The research is carried out through a case study at a case company labelled as a high technology scaleup. Interviews were conducted with sales representatives working in the commercial team at the case company. The result from this research finds that six steps are included in the B2B sales process at high technology scaleups. The steps are as follows: Lead generation, First meeting, Assessment, Contract proposal, Negotiation and Closed deal. The second step includes presenting the offered product or service, which this research identified as most challenging for the sales representatives to execute successfully due to the technical complexity of the product/service. Findings from this research shows that data driven customer insights can be used to simplify this step in the process. For example, data driven customer insights can help personalize presentation material and enable rapport building. In addition, data driven customer insights help align expectations between buyers and sellers during the first meeting, thus increasing the likelihood of reaching a closed deal. / När ett företag ska skalas upp är det viktigt att implementera kundinsikter för att uppnå ökad omsättning. Att förstå och definiera en passande B2B-försäljningsprocess har också visats spela en viktig roll för nå ökade intäkter, och traditionella säljprocesser innehåller flera steg som säljpersonal utför. Ett steg kretsar kring presentationen av den erbjudna produkten eller tjänsten. För att säljpersonal ska kunna presentera en produkt eller tjänst med framgång behöver de förvärva eller ha djup kunskap om kunden, såsom branschtrender och generell verksamhet. Detta kan uppnås genom att anskaffa kundinsikter som är datadrivna. Att använda datadriven kundinsikt har också visats öka försäljningssiffror. Med detta som bakgrund undersöker därför den här forskningen sambandet mellan B2B-försäljningsprocessen och generering och implementering av datadriven kundinsikt. I synnerhet undersöker denna forskning stegen som ingår i en B2B-försäljningsprocess i ett högteknologiskt scale up och därmed hur datadriven kundinsikt kan förbättra presentationssteget i B2B-försäljningsprocessen.  Forskningen utförs genom en fallstudie på ett fallföretag som räknas som ett högteknologiskt scale up. Intervjuer genomfördes med försäljningsmedarbetare som jobbar i det kommersiella teamet på företaget. Resultatet från denna forskning visar att sex steg ingår i B2B-försäljningsprocessen vid högteknologiska scale ups. Dessa sex stegen är: Leadsgenerering, Första möte, Utvärdering, Kontraktsförslag, Förhandling och Avslutad affär. Det andra steget innebär att den erbjudna produkten eller tjänsten presenteras, och detta steg identifieras som mest utmanande för försäljningsmedarbetarna att utföra med framgång på grund av produktens/tjänstens tekniska komplexitet. Vidare visar resultat från denna forskning att datadriven kundinsikt kan användas för att förenkla detta steg i processen. Datadriven kundinsikt kan till exempel hjälpa till att personalisera presentationsmaterial och möjliggöra förtroendebyggande. Dessutom möjliggör datadrivna kundinsikter att köpare och säljare delar gemensamma förväntningar på det första mötet, vilket ökar sannolikheten att uppnå en sluten affär.

<p><a href="" target="_blank">Synthetic microbial communities (SynComs) are an important focus in modern microbiology. SynComs are used for studying the dynamics of naturally occurring microbial communities ranging from soil to the human gut. Synthetic refers to the assemblage of some or all the members of these communities in a laboratory setting. SynComs allow for communities difficult to study <i>in situ</i> to be studied in a controlled environment, or they may be used to create beneficial products like biofertilizers. When SynComs are used as products, the focus shifts to optimizing a desired outcome of a culture. For biofertilizers, a high diversity is key to producing a functionally redundant product for stimulating plant growth. Media manipulation is a common approach for inducing community changes in a SynCom. Amino acids (AA) are a media supplement that soil microbes (often the components of biofertilizers) are particularly fond of to support their metabolic activities. This study took a scale-up approach to assess the changes in growth dynamics of a SynCom (Environoc© 401) when supplemented with different concentrations of commercially available AA products from plant and animal sources. Expanding from microplates, to shake flasks, then into a 4L bioreactor, Environoc© 401 cultures were compared for their maximum growth rate, time in lag phase, and final growth (optical density or viable cell density) when supplemented with these AA products at various concentrations. Furthermore, Illumina sequencing of the 16S rRNA gene was used to evaluate community-level changes from these treatments based on taxonomic, alpha (Chao 1 and Shannon indices), and beta diversity (Generalized UniFrac) of shake flask and bioreactor samples. Quantitative PCR was also used to assess the relative change of three select species of the SynCom in each AA treatment. Shake flask data revealed significant changes in the growth dynamics of the SynComs within AA treatment groups. Whether the AA was animal or plant derived, generally as the concentration of AA increased, the maximum growth rate decreased, lag time increased, and final growth readings increased. The best AA supplement and rate according to the growth metrics and <i>Curveball </i>analysis was Stimtide at a 33% supplementation rate. This was compared with the control at the bioreactor scale where it showed higher overall final growth and a higher taxonomic and alpha diversity. The scale-up approach to this study was successful at selecting the best amino acid supplement type and rate despite having less sophisticated control and monitoring compared to larger scales (i.e. the bioreactor). Use of the <i>Curveball</i> modeling program was useful for treatment group selection but did not always predict the outcomes seen in the live cultures. The use of AA as a media supplement can increase growth and diversity of a SynCom, though not all AA supplements or rates affect growth and community dynamics in the same way</a>.</p>

Dans le contexte énergétique et climatique mondial, le coût élevé des enzymes Cellulolytiques (cellulases) freine le développement des bioraffineries lignocellulosiques, pour produire des biocarburants et composés chimiques à partir d'une matière première végétale renouvelable. L'objectif de ce travail est de caractériser et de modéliser le métabolisme du micro-organisme Trichoderma reesei, afin d'optimiser le protocole industriel de production de cellulases. Cette étude a été réalisée sur des milieux modèles représentatifs de ceux attendus à l'échelle industrielle. Tout d'abord, la stoechiométrie des réactions de croissance et de production a été établie, puis une étude cinétique a été menée pour mesurer précisément le comportement du micro-organisme à forte induction de la production de cellulases. Le modèle résultant a été utilisé pour optimiser le protocole industriel de production. Ensuite, l'intégration de cette étape dans une bioraffinerie lignocellulosique a été étudiée, avec l'effet sur le métabolisme i) des mélanges de sucres disponibles, ii) des composés inhibiteurs issus de la dégradation de la lignocellulose, et iii) du changement d'échelle. Ces travaux ont fait progresser de façon substantielle les connaissances du métabolisme de T. reesei en ce qui concerne la production de cellulases, et les modèles développés sont des outils d'aide rationnelle à la définition d'un procédé de production de cellulases intégré dans une bioraffinerie lignocellulosique. / In the global energetic and climatic context, the high cost of the cellulolytic enzymes (cellulases) postpones the development of lignocellulosic biorefineries, dedicated to produce biofuels and chemical compounds from renewable vegetable feedstocks. The aim of this work was to measure and model the metabolism of the micro-organism Trichoderma reesei, in order to optimize the industrial protocol for the production of cellulase. This study was carried out using synthetic media representative of industrial ones. First, the stoichiometries of growth and protein production reactions were determined. Then, a kinetic study was conducted to precisely measure the specific rates of T. reesei at high induction of cellulase production. The resulting model was used to optimize the industrial production protocol. Finally the integration of this step in a lignocellulosic biorefinery was studied by determining the impacts on the metabolism of i) available sugar mixtures, ii) inhibitory compounds from lignocellulosic biomass degradation, and iii) scale-up. These results significantly contributed to improve the knowledge of T. reesei metabolism on cellulase production. The developed models are rational tools for the optimization of a cellulase production protocol suited to lignocellulosic biorefineries.

O desenvolvimento de fontes de energia renováveis tem se tornado cada vez mais necessário e, nesse contexto, os biocombustíveis têm um importante papel. Este trabalho teve como objetivo otimizar a produtividade de biomassa e de lipídios da microalga Nannochloropsis gaditana, em cultivos de baixo-custo visando à produção de biodiesel. Um sistema de cultivo foi concebido com materiais acessíveis e os experimentos testaram o efeito de diferentes exposições das células ao claro-escuro, sob irradiância de 800?E m-2 s-1. Foram testadas três profundidades de cultivo (2, 4 e 8 cm) e duas taxas de aeração (0,25 e 1,00 L min-1 por L de cultivo). A penetração de luz nos cultivos e o rendimento de biomassa seca foram monitorados diariamente. Altos rendimentos de biomassa e lipídios foram alcançados. Os valores máximos foram observados no experimento com 4 cm de profundidade e maior taxa de aeração: 13,83 g m-2 d-1 de produtividade de biomassa e 20,50% de teor lipídico, resultando em uma produtividade lipídica de 2,84 g m-2 d-1. Estes resultados indicam o potencial da utilização de N. gaditana para aplicação em cultivos de larga escala, uma vez que o rendimento lipídico observado foi superior ao da soja, principal matéria-prima vegetal para produção de biodiesel. O sistema de cultivo desenvolvido pode ser inserido em um processo de scaling up em cultivos abertos de grande escala. / The development of renewable energy sources has becoming an increasing necessity and, in this context, biofuels have an important role. This study aimed to optimize the productivity of biomass and lipids of the microalgae Nannochloropsis gaditana in a low-cost system, focusing biofuel production. A culture system was designed with low-cost and accessible materials and the experiments tested the effect of different degrees of exposition of cells to light-dark, under 800?E m-2 s-1 irradiance. Three different culture depths (2, 4 e 8 cm) and two aeration ratios (0.25 e 1.00 L min-1 per L of culture) were tested. Light penetration into the cultures and the dry biomass yield were monitored daily. High yields of biomass and lipids were attained in the experiment with 4 cm depth and the high aeration ratio: 13.83g m-2 d-1 of biomass productivity and 20.50% lipid content, resulting in a lipid productivity of 2.84 g m-2 d-1. These results indicate the potential of N. gaditana for application in large-scale cultivation, since its lipid yield is higher than the soybean, the main feedstock plant for biodiesel production. The culture system developed can be inserted in a process of biomass scaling up for large-scale open microalgae cultures.

"The next generation of aluminum alloys will have to operate at temperatures approaching 300°C. Traditional aluminum alloys cannot perform at these temperatures, but aluminum alloys reinforced with fine ceramic particles can. The objective of this research is to develop a process to synthesize Al-TiC composites by the Rotating Impeller Gas-Liquid In-situ method. This method relies on injecting methane into molten aluminum that has been pre-alloyed with titanium. The gas is introduced by means of a rotating impeller into the molten alloy, and under the correct conditions of temperature, gas flow, and rotation speed, it reacts preferentially with titanium to form titanium carbide particles. The design of the apparatus, the multi-physics phenomena underlying the mechanism responsible for particle formation and size control, and the operation window for the process are first elucidated. Then a parametric study that leads to the synthesis of aluminum reinforced with TiC microparticles and nanoparticles is described. Finally, potential technical obstacles that may stand in the way of commercializing the process are discussed and ways to overcome them are proposed. "

O desenvolvimento de fontes de energia renováveis tem se tornado cada vez mais necessário e, nesse contexto, os biocombustíveis têm um importante papel. Este trabalho teve como objetivo otimizar a produtividade de biomassa e de lipídios da microalga Nannochloropsis gaditana, em cultivos de baixo-custo visando à produção de biodiesel. Um sistema de cultivo foi concebido com materiais acessíveis e os experimentos testaram o efeito de diferentes exposições das células ao claro-escuro, sob irradiância de 800?E m-2 s-1. Foram testadas três profundidades de cultivo (2, 4 e 8 cm) e duas taxas de aeração (0,25 e 1,00 L min-1 por L de cultivo). A penetração de luz nos cultivos e o rendimento de biomassa seca foram monitorados diariamente. Altos rendimentos de biomassa e lipídios foram alcançados. Os valores máximos foram observados no experimento com 4 cm de profundidade e maior taxa de aeração: 13,83 g m-2 d-1 de produtividade de biomassa e 20,50% de teor lipídico, resultando em uma produtividade lipídica de 2,84 g m-2 d-1. Estes resultados indicam o potencial da utilização de N. gaditana para aplicação em cultivos de larga escala, uma vez que o rendimento lipídico observado foi superior ao da soja, principal matéria-prima vegetal para produção de biodiesel. O sistema de cultivo desenvolvido pode ser inserido em um processo de scaling up em cultivos abertos de grande escala. / The development of renewable energy sources has becoming an increasing necessity and, in this context, biofuels have an important role. This study aimed to optimize the productivity of biomass and lipids of the microalgae Nannochloropsis gaditana in a low-cost system, focusing biofuel production. A culture system was designed with low-cost and accessible materials and the experiments tested the effect of different degrees of exposition of cells to light-dark, under 800?E m-2 s-1 irradiance. Three different culture depths (2, 4 e 8 cm) and two aeration ratios (0.25 e 1.00 L min-1 per L of culture) were tested. Light penetration into the cultures and the dry biomass yield were monitored daily. High yields of biomass and lipids were attained in the experiment with 4 cm depth and the high aeration ratio: 13.83g m-2 d-1 of biomass productivity and 20.50% lipid content, resulting in a lipid productivity of 2.84 g m-2 d-1. These results indicate the potential of N. gaditana for application in large-scale cultivation, since its lipid yield is higher than the soybean, the main feedstock plant for biodiesel production. The culture system developed can be inserted in a process of biomass scaling up for large-scale open microalgae cultures.