Dans le but d'accéder à un radioimmunodosage simultané des 25-hydroxyvitamines D2 et D3, qui sont un bon indicateur de la quantité de vitamine D disponible dans l'organisme, 5 haptènes de la vitamine D de chaînons variables à fonction acide carboxylique terminale ont été préparés par hémisynthèse à partir de la chaîne latérale de la vitamine D2. Ces 5 haptènes ont été couplés à la sérum albumine bovine pour immuniser des souris, des rats et des lapins ou bien à l'histamine pour donner des traceurs radioactifs, marqués par l'iode (125I). Les animaux ont bien répondu en sécrétant des anticorps dirigés contre les deux 25-hydroxyvitamines D2 et D3 avec des reconnaissances presque identiques. Les cellules spléniques de 3 de ces rats ont pu être fusionnées avec un myélome de souris afin de les immortaliser. Deux hybridomes sécréteurs d'anticorps monoclonaux qui reconnaissent les deux vitamines similairement et ne croisent pas avec l'hormone 1α,25-dihydroxyvitamine D ont pu être sélectionnés.

Vitamin D deficiency and insufficiency are now seen as a contemporary health problem in Australia with possible widespread health effects not limited to bone health1. Despite this, the Vitamin D status (measured as serum 25-hydroxyvitamin D (25(OH)D)) of ambulatory adults has been overlooked in this country. Serum 25(OH)D status is especially important among this group as studies have shown a link between Vitamin D and fall risk in older adults2. Limited data also exists on the contributions of sun exposure via ultraviolet radiation and dietary intake to serum 25(OH)D status in this population. The aims of this project were to assess the serum 25(OH)D status of a group of older ambulatory adults in South East Queensland, to assess the association between their serum 25(OH)D status and functional measures as possible indicators of fall risk, obtain data on the sources of Vitamin D in this population and assess whether this intake was related to serum 25(OH)D status and describe sun protection and exposure behaviors in this group and investigate whether a relationship existed between these and serum 25(OH)D status. The collection of this data assists in addressing key gaps identified in the literature with regard to this population group and their Vitamin D status in Australia. A representative convenience sample of participants (N=47) over 55 years of age was recruited for this cross-sectional, exploratory study which was undertaken in December 2007 in south-east Queensland (Brisbane and Sunshine coast). Participants were required to complete a sun exposure questionnaire in addition to a Calcium and Vitamin D food frequency questionnaire. Timed up and go and handgrip dynamometry tests were used to examine functional capacity. Serum 25(OH)D status and blood measures of Calcium, Phosphorus and Albumin were determined through blood tests. The Mean and Median serum 25-Hydroxyvitamin D (25(OH)D) for all participants in this study was 85.8nmol/L (Standard Deviation 29.7nmol/L) and 81.0nmol/L (Range 22-158nmol/L), respectively. Analysis at the bivariate level revealed a statistically significant relationship between serum 25(OH)D status and location, with participants living on the Sunshine Coast having a mean serum 25(OH)D status 21.3nmol/L higher than participants living in Brisbane (p=0.014). While at the descriptive level there was an apparent trend towards higher outdoor exposure and increasing levels of serum 25(OH)D, no statistically significant associations between the sun measures of outdoor exposure, sun protection behaviors and phenotypic characteristics and serum 25(OH)D status were observed. Intake of both Calcium and Vitamin D was low in this sample with sixty-eight (68%) of participants not meeting the Estimated Average Requirements (EAR) for Calcium (Median=771.0mg; Range=218.0-2616.0mg), while eighty-seven (87%) did not meet the Adequate Intake for Vitamin D (Median=4.46ug; Range=0.13-30.0ug). This raises the question of how realistic meeting the new Adequate Intakes for Vitamin D is, when there is such a low level of Vitamin D fortification in this country. However, participants meeting the Adequate Intake (AI) for Vitamin D were observed to have a significantly higher serum 25(OH)D status compared to those not meeting the AI for Vitamin D (p=0.036), showing that meeting the AI for Vitamin D may play a significant role in determining Vitamin D status in this population. By stratifying our data by categories of outdoor exposure time, a trend was observed between increased importance of Vitamin D dietary intake as a possible determinant of serum 25(OH)D status in participants with lower outdoor exposures. While a trend towards higher Timed Up and Go scores in participants with higher 25(OH) D status was seen, this was only significant for females (p=0.014). Handgrip strength showed statistically significant association with serum 25(OH)D status. The high serum 25(OH)D status in our sample almost certainly explains the limited relationship between functional measures and serum 25(OH)D. However, the observation of an association between slower Time Up and Go speeds, and lower serum 25(OH)D levels, even with a small sample size, is significant as slower Timed Up and Go speeds have been associated with increased fall risk in older adults3. Multivariable regression analysis revealed Location as the only significant determinant of serum 25(OH)D status at p=0.014, with trends (p=>0.1) for higher serum 25(OH)D being shown for participants that met the AI for Vitamin D and rated themselves as having a higher health status. The results of this exploratory study show that 93.6% of participants had adequate 25(OH)D status-possibly due to measurement being taken in the summer season and the convenience nature of the sample. However, many participants do not meet their dietary Calcium and Vitamin D requirements, which may indicate inadequate intake of these nutrients in older Australians and a higher risk of osteoporosis. The relationship between serum 25(OH)D and functional measures in this population also requires further study, especially in older adults displaying Vitamin D insufficiency or deficiency.

Univ., Diss.--Mainz, 2002.

Made available in DSpace on 2015-12-06T23:44:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2006 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Objetivos: Avaliar os efeitos de 6 meses de suplementação com colecalciferol e cálcio sobre o metabolismo mineral e sobre os parâmetros de força muscular de membros inferiores, oscilação postural e mobilidade funcional. Desenho do Estudo: Ensaio clínico prospectivo, randomizado, duplo-cego, placebocontrolado. Local de realização: Duas instituições de longa permanência para idosos, em São Paulo - SP, Brasil. Participantes: 56 idosos de ambos os sexos (12 homens e 44 mulheres), com 60 anos de idade ou mais (mediana=77,6; limites=62-94 anos). Métodos: Os pacientes foram randomizados em Grupo-Ca (n=28) para placebo, ou Grupo-Ca+D (n=28) para colecalciferol. Todos os participantes receberam 1000 mg/dia de cálcio. O Grupo-Ca+D recebeu colecalciferol oral nas doses de 150.000 UI/ mês durante os 2 primeiros meses de estudo e 90.000 UI/mês nos 4 meses subseqüentes, correspondendo a uma dose mensal de 3670 UI/dia em média, de Dezembro-2004 a Maio-2005. Níveis séricos de 25-Hidroxivitamina D (25OHD), paratormônio intacto (PTH) e cálcio foram mensurados no início do estudo (M1), 2 meses (M2) e 6 meses (M3) após tratamento. Os testes neuromusculares foram realizados antes do início da intervenção e repetidos após o fim do tratamento. A força muscular dos membros inferiores foi avaliada através de um índice de força muscular (IFM), incluindo a força dos músculos flexores do quadril e extensores do joelho, mensurada por dinamômetro mecânico portátil. Para avaliar a oscilação postural foi criado um índice (IOP) a partir da mensuração da oscilação do corpo nos diâmetros sagital e frontal ao nível da cintura. A mobilidade funcional foi mensurada através dos testes “Timed Up&Go” (TUG) e alcance funcional (TAF). Resultados: A 25OHD sérica aumentou em ambos os grupos no M2, porém mais no Grupo-Ca+D do que no Grupo-Ca (OR=2,2; 95%IC=1,98-2,4 vs. OR=1,76; 95%IC=1.55-1.99, respectivamente). No M3, os níveis de 25OHD declinaram apenas no Grupo-Ca, contudo, o PTH sérico diminuiu no M2 (p<0.0001) e retornou aos valores basais no M3 (p<0.0001) igualmente nos dois grupos. Antes do tratamento, deficiência/insuficiência de 25OHD (<50 nmol/L) afetava 67,9% do total de participantes. No M3, nenhum paciente do Grupo-Ca+D, mas 40% dos pacientes do Grupo-Ca tinham deficiência/insuficiência de 25OHD. Hipercalcemia não foi detectada em nenhum paciente. Apenas no Grupo-Ca+D, o IFM teve um aumento de 20% no M3 (OR=1,20; 95%IC=1,12-1,29), enquanto que IOP e TAF aumentaram igualmente nos dois grupos, provavelmente porque os pacientes de ambos os grupos aumentaram sua exposição solar durante o verão. Conclusões: A suplementação com colecalciferol e cálcio foi segura e efetiva em aumentar os níveis séricos de 25OHD, reduzir a prevalência de deficiência/insuficiência de 25OHD e aumentar a força muscular de membros inferiores nos idosos do grupo tratado. Palavras-chave: 25-Hidroxivitamina D, colecalciferol, idosos, força muscular, oscilação postural, mobilidade funcional. / Objectives: To assess the effects of a 6-month supplementation with vitamin D and calcium on mineral metabolism and parameters of lower-extremity muscle-strength, body sway (BS) and functional mobility, measured by the Functional Reach Test (FRT) and Timed Up&Go test (TUG). Design: Prospective, double-blind, placebo-controlled trial. Setting: Institutionalized elderly of two long-stay geriatric care units of São Paulo-SP, Brazil. Participants: 56 elderly volunteers of both genders (12 men and 44 women) of ages 60 and older (median=77.6; range=62-94 years). Methods: Subjects were randomized into a Ca-group (n=28) to receive placebo or a Ca+D-group (n=28) to receive cholecalciferol. All participants received 1,000 mg/day of calcium. Laboratory measurements were performed at baseline (M1), 2 moths (M2) and 6 months (M3) after intervention. The Ca+D-group received oral cholecalciferol on a monthly basis (3670 IU/day on average, from December-2004 to May-2005). Neuromuscular measurements were performed at baseline and 6 months. Results: Serum 25(OH)D increased in both groups at M2, but more so in the Ca+Dgroup than in the Ca-group (OR=2.2, 95%CI=1.98-2.4 vs. OR=1.76, 95%CI=1.55- 1.99, respectively). At M3, 25(OH)D levels declined only in the Ca-group. Nevertheless, serum PTH diminished at M2 (p<0.0001) and went back to baseline levels at M3 (p<0.0001) equally in both groups. Before treatment, 25(OH)D deficiency/insufficiency (<50 nmol/liter) affected 67.9% of the entire group. At M3, no patient in the Ca+D-group, but 40% of the Ca-group patients had 25(OH)D deficiency/insufficiency. Hypercalcemia was not detected at any time. The odds of improving lower-extremity muscle strength increased by 20% (OR=1.20, 95%CI=1.12-1.29) only in the Ca+D-group, whereas BS and FRT increased equally in both groups, probably because the study was conducted during the summer. Conclusions: The supplementation with calcium and supra-physiological doses of cholecalciferol was safe and effective in enhancing 25(OH)D levels, reducing the prevalence of 25(OH)D insufficiency, and increasing lower-extremity muscle strength in institutionalized elderly. / FAPESP: 03/13194-6 / BV UNIFESP: Teses e dissertações

A crescente complexidade das redes de comunicação de dados tem como conseqüência direta tornar cada vez mais complexas as tarefas de projetar seu dimensionamento e evolução. Um passo preliminar vitalmente importante no projeto de uma rede é a coleta de dados relacionados ao uso esperado da rede. Os padrões de tráfego estimados são usados nos cálculos de dimensionamento dos recursos. Fortemente relacionada a estas atividades está a previsão da performance em termos de throughput da rede, tempos de resposta, probabilidade de congestionamento, entre outras. Uma análise de performance efetiva depende de se representar precisamente a configuração da rede e da carga de tráfego a ela submetida. Técnicas de modelagem analítica ou de simulação podem ser usadas para determinar a performance esperada do sistema. Neste trabalho a usada a técnica de simulação para modelar o tráfego da rede usando dados coletados em uma rede real por monitoração. Trabalhando-se com o modelo validado e possível ajustar a carga de tráfego para representar mudanças esperadas nos volumes de tráfego, e testar o efeito na performance de diferentes configurações de rede. Isso possibilita que os processos de planejamento e projeto sejam executados com confiança, alem dos custos da rede poderem ser otimizados. O sistema apresentado neste trabalho e orientado à gerência de performance de redes, apoiando as atividades de gerência e planejamento de capacidade. Defende-se a idéia de que, a partir do perfil dos usuários de uma rede, derivado do tráfego por eles gerado, pode-se construir um modelo de tráfego significativamente representativo para a análise e previsão do desempenho da rede. O modelo foi desenvolvido para redes de comutação de pacotes com serviço orientado a conexão, e validado sobre o tráfego de uma rede X.25 através de um modelo de simulação, implementado em GPSS. O protótipo do sistema é composto por três processos principais que incorporam as etapas da análise de performance: monitoração do tráfego, análise do tráfego e simulação do tráfego. Na monitoração, são contabilizadas uma série de variáveis para cada conexão gerada na rede. Para cada tipo de aplicação são calculados os respectivos fatores de carga de tráfego. As aplicações são classificadas em dois grandes grupos: interativas e não-interativas. O processo de análise do tráfego gera os fatores de carga de tráfego na forma de tabelas e gráficos. As conexões e as métricas de performance produzidas na simulação do tráfego podem ser analisadas e validadas graficamente. O sistema proposto foi especificado em SDL (Specification and Description Language). / The increasing complexity of communication networks turns each time more complex the task to plan its expansion and evolution. A vitally important preliminar step in computer network design is the gathering of data relating to the expected use of the network. The estimated traffic patterns are used in resource usage computations. Closely related to these activities is the estimation of performance in terms of network throughput, response times, congestion probability, among others. An efective performance analysis requires the accurate representation of computer network configuration and traffic load. Analytical or simulation techniques can be used to establish the expected system performance. This work makes use of simulation to model the network traffic, based on collected data of a real network. network. Working with the validated model is possible to adjust the traffic load to represent future demand, and to test the performance impact under distinct network configurations. This enable a trustful execution of network planning and design, besides of the cost optimization. The system presented at this work is network performance management oriented, giving support to capacity management and capacity planning. The central idea is that, through network users profile achieved from generated traffic, it's possible to build a representative traffic model to aim at the network performance analysis and forecast. The model was developed to connection oriented packet switched networks, and validated by means of a simulation model to X.25 network traffic, implementd in GPSS. The system prototype comprises three process: traffic monitoring, traffic analysis and traffic simulation. In monitoring, a set of variables are registered for each network connection generated. The traffic load factors are computed according the application type. The applications are classified in two groups: interactive and non-interactive. The traffic analysis process produces traffic load factors in form of tables and graphics. Connections and performance metrics generated at traffic simulation can be graphically analyzed and validated. The proposed system was specified in SDL (Specification and Description Language).

A crescente complexidade das redes de comunicação de dados tem como conseqüência direta tornar cada vez mais complexas as tarefas de projetar seu dimensionamento e evolução. Um passo preliminar vitalmente importante no projeto de uma rede é a coleta de dados relacionados ao uso esperado da rede. Os padrões de tráfego estimados são usados nos cálculos de dimensionamento dos recursos. Fortemente relacionada a estas atividades está a previsão da performance em termos de throughput da rede, tempos de resposta, probabilidade de congestionamento, entre outras. Uma análise de performance efetiva depende de se representar precisamente a configuração da rede e da carga de tráfego a ela submetida. Técnicas de modelagem analítica ou de simulação podem ser usadas para determinar a performance esperada do sistema. Neste trabalho a usada a técnica de simulação para modelar o tráfego da rede usando dados coletados em uma rede real por monitoração. Trabalhando-se com o modelo validado e possível ajustar a carga de tráfego para representar mudanças esperadas nos volumes de tráfego, e testar o efeito na performance de diferentes configurações de rede. Isso possibilita que os processos de planejamento e projeto sejam executados com confiança, alem dos custos da rede poderem ser otimizados. O sistema apresentado neste trabalho e orientado à gerência de performance de redes, apoiando as atividades de gerência e planejamento de capacidade. Defende-se a idéia de que, a partir do perfil dos usuários de uma rede, derivado do tráfego por eles gerado, pode-se construir um modelo de tráfego significativamente representativo para a análise e previsão do desempenho da rede. O modelo foi desenvolvido para redes de comutação de pacotes com serviço orientado a conexão, e validado sobre o tráfego de uma rede X.25 através de um modelo de simulação, implementado em GPSS. O protótipo do sistema é composto por três processos principais que incorporam as etapas da análise de performance: monitoração do tráfego, análise do tráfego e simulação do tráfego. Na monitoração, são contabilizadas uma série de variáveis para cada conexão gerada na rede. Para cada tipo de aplicação são calculados os respectivos fatores de carga de tráfego. As aplicações são classificadas em dois grandes grupos: interativas e não-interativas. O processo de análise do tráfego gera os fatores de carga de tráfego na forma de tabelas e gráficos. As conexões e as métricas de performance produzidas na simulação do tráfego podem ser analisadas e validadas graficamente. O sistema proposto foi especificado em SDL (Specification and Description Language). / The increasing complexity of communication networks turns each time more complex the task to plan its expansion and evolution. A vitally important preliminar step in computer network design is the gathering of data relating to the expected use of the network. The estimated traffic patterns are used in resource usage computations. Closely related to these activities is the estimation of performance in terms of network throughput, response times, congestion probability, among others. An efective performance analysis requires the accurate representation of computer network configuration and traffic load. Analytical or simulation techniques can be used to establish the expected system performance. This work makes use of simulation to model the network traffic, based on collected data of a real network. network. Working with the validated model is possible to adjust the traffic load to represent future demand, and to test the performance impact under distinct network configurations. This enable a trustful execution of network planning and design, besides of the cost optimization. The system presented at this work is network performance management oriented, giving support to capacity management and capacity planning. The central idea is that, through network users profile achieved from generated traffic, it's possible to build a representative traffic model to aim at the network performance analysis and forecast. The model was developed to connection oriented packet switched networks, and validated by means of a simulation model to X.25 network traffic, implementd in GPSS. The system prototype comprises three process: traffic monitoring, traffic analysis and traffic simulation. In monitoring, a set of variables are registered for each network connection generated. The traffic load factors are computed according the application type. The applications are classified in two groups: interactive and non-interactive. The traffic analysis process produces traffic load factors in form of tables and graphics. Connections and performance metrics generated at traffic simulation can be graphically analyzed and validated. The proposed system was specified in SDL (Specification and Description Language).

Bakgrund: Vitamin D finns i två olika former, det animaliska D3 (kolekalciferol) och det vegetabiliska D2 (ergokalciferol). Det har rått olika åsikter bland läkarkåren och allmänheten om vilken av de två formerna som är mest potent för att höja 25(OH)D-statusen i blodet, det värde som mäts för att avgöra vitamin D-halten i kroppen. Då vitamin D-brist är vanligt förekommande bland befolkningen i Norden är det viktigt att veta vilken form som har bäst effekt och som därför bör användas för att behandla och förebygga vitamin D-brist. Det har även betydelse för veganer som inte äter det animaliska D3, där rekommendationen kanske behöver ändras. Syfte: Syftet med studien var att ta reda på om det finns någon skillnad i potensen av D2 respektive D3 för att höja 25(OH)D-statusen i blodet och i så fall, att hitta möjliga orsaker till denna skillnad. Metod: Sex relevanta vetenskapliga originalartiklar, som har undersökt effekten av D2 vs. D3 på 25(OH)D-statusen i blodet, hittades i databasen PubMed. Studierna genomfördes mellan 2008 och 2017. Studiedeltagarna var vuxna friska människor. Resultat: Fyra av studierna pekade på att D3 var mer effektivt än D2 för att höja 25(OH)D-statusen. En studie kom fram till att det inte fanns någon skillnad i potensen mellan D2 och D3 och en studie visade att D2 var mer effektivt jämfört med D3 när det gällde daglig behandling med låga doser men att D3 uppvisade bättre effekt vid behandling med höga doser med två eller fyra veckors avstånd. Slutsats: Majoriteten av studierna visade en bättre effekt av D3 än D2 för att höja 25(OH)D-nivåer i blodet. De blandade resultaten samt det begränsade antalet studier och deltagare gör att det inte är möjligt att kunna komma fram till en tydlig slutsats. / Background: Vitamin D comes in two different forms, D3 from animals (cholecalciferol) and D2 from plants (ergocalciferol). There has been different opinions among physicians and the general public about which of the calciferols is more potent to raise 25(OH)D-levels in the blood, which is the value that is measured to determine the vitamin D-status in the body. Since vitamin D deficiency is common among the people of the Nordic countries it is important to know which form has the best effect and should be used to treat and prevent vitamin D deficiency. Furthermore, it is relevant for vegans who do not eat the animalic D3, where recommendations might have to be changed. Aim: The aim of the study was to find out if there were differences in potency of D2 vs. D3 to raise 25(OH)D status in the blood and if so, to find possible explanations for those differences. Methods: Six relevant original articles that examined the effect of D2 vs. D3 on 25(OH)D status in the blood, were found in the database PubMed. The studies where published between the years 2008 and 2017. The participants were healthy adults. Results: Four of the studies suggested that D3 is more effective than D2 in order to raise the 25(OH)D status. One study concluded that there is no difference in the effectiveness of D2 vs D3 and one study showed that D2 is more effective than D3 when it comes to daily treatment but that D3 has a better effect than D2 when treatment happens on a two or four weekly basis with large doses. Conclusion: Most of the articles suggested a better effectiveness of D3 than D2 to raise 25(OH)D levels in the blood. However, besides the mixed results, the number of studies and participants was too small to come to a clear conclusion.

I nuläget finns det begränsat med forskning hur det går för de personer som opererar bort ett öga. Detta kan medföra att ögonsjuksköterskan ger otillräcklig information till patient och anhöriga i samband med att det är aktuellt att bära protes. Därför var det av intresse att undersöka den synrelaterade livskvaliteten hos personer som genomgått en ögonamputation. Syftet var att belysa synrelaterad livskvalitet hos personer som genomgått en ögonamputation och har en ögonprotes. Studien är en tvärsnittsstudie med deskriptiv design. Datainsamlingen omfattade frågeformuläret NEI-VFQ-25 med tilläggsfrågor, där deltagarna var vuxna personer som genomgått en ögonamputation de senaste fem åren. Resultatet visade att deltagarna hade lägre synrelaterad livskvalitet än personer i kontrollgruppen från H70-studien 2014/2015 med och utan påverkan på synförmågan. Mest besvär hade deltagarna med sidoseende och aktiviteter både på nära och långt håll. Resultatet av studien kan ge ögonsjuksköterskan fördjupad kunskap som kan användas i personcentrerad vård som stöd för att uppmuntra personen som genomgått en ögon amputation och att på bästa sätt hantera sin nya livssituation. Forskning omfattande synrelaterad livskvalitet hos samtliga vuxna personer i Sverige som genomgått en ögonamputation och har ögonprotes vore av intresse, eftersom resultatet baseras på en grupp patienter vid en ögonklinik. / At the moment there is little research about the outcome of people who have had an eye surgically removed. This may result in an ophthalmic nurse giving insufficient information to patients and relatives. Therefore, it would be interesting to investigate the vision related quality of life in relation to the eyesight of persons who have undergone an eye amputation. The purpose was to illustrate the vision related quality of life of people who have been subject to an eye amputation and have an eye prothesis. The study is a cross-sectional study with a descriptive design. The data collection was conducted using the questionnaire NEI-VFQ-25 together with supplementary questions which was sent to adults who have undergone an eye amputation in the last five years. The results showed that eye amputees had lower vision-related quality of life than participants in a control group from the H70 study 2014/2015 with and without visual symptoms. The biggest inconvenience for the participants was peripheral vision and both near activities and distance activities. The results of the study can provide the ophthalmic nurse with a deeper knowledge to be used working with personcentered care and provide thourough information to support persons how has went through an eye amputation to manage their new life situation. Further research on eye amputees vision-related quality of life nationally is needed because the result is based on a limited study group.

The Description of the control system Heidenhain iTNC 530 and the machine FV 25 CNC A. Detailed analysis of the impact of single programming functions for more effectivity machining by the help of specified machine.

A project developed during a magister´s study of a program M-STM Mechanical engineering and industrial management brings in a proposal of a technology of cold forging part out of 12 040 steel. The forging on a multi-stage processual automatic machine TPZK 25 with nominal forming power 5000 kN was projected based on consultations, study of literary resources a calculations.