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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
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Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 11 to 20 of 25 (0.011 seconds)| 11 |
STED nanoscopy of the living brain / STED-Mikroskopie des intakten GehirnsBerning, Sebastian 13 December 2011 (has links)
No description available.
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Entwicklung und Einsatz der Immun-SERS-Mikroskopie zur Gewebe-basierten TumordiagnostikSalehi, Mohammad 09 September 2013 (has links)
Surface-enhanced Raman scattering (SERS) microscopy is a novel method of optical imaging for the localization and quantification of target molecules in cells and tissue specimens. The major advantages of SERS over fluorescence are quantification and spectral multiplexing due to the small line width of vibrational Raman bands. The position of the plasmon band of both hollow gold/silver nanoshells and silica-encapsulated gold nanoclusters can be tuned for maximum SERS enhancement upon red laser excitation, which is optimal for minimizing the disturbing autofluorescence of tissue. In this work, silica-encapsulated and non-encapsulated SERS particles were used for the localization of target proteins in prostate tissue specimens. Two different biofunctionalization methods were established for each type of SERS particles. The cross-linking method based on s-NHS/EDC chemistry was modified for covalently conjugating proteins to hollow gold/silver nanoshells and gold nanostars in order to minimize the aggregation of SERS nanoparticles during and after cross-linking. As an alternative to covalent conjugation chemistry, the noncovalent binding of antibodies to the SERS particles via an adapter protein (protein A/G) was established. The influence of several factors that determine the quality of results obtained by SERS imaging, such as the number of immuno-SERS conjugates, incubation time, antigen retrieval and blocking buffer, were investigated. Rapid SERS microscopy with 30 msec acquisition time per pixel was enabled by using silica-encapsulated gold nanoclusters for the localization of p63 proteins on prostate tissue specimens from healthy donors. Two-color SERS experiments for the parallel localization of PSA and p63 were performed with silica-encapsulated and non-encapsulated nanoshells. The quality of the results depends less on the nature of the surface chemistry of the nanoparticles (with or without silica encapsulation), but more on the blocking buffer and the antigen retrieval method. Silica-encapsulated gold nanoclusters were also used for the simultaneous quantification of three cytokines (IL1, IL8 and TNF- α) in a SERS-based sandwich immunoassay with a detection limit of ca. 0.3 pM.
Keywords: Raman, SERS microscopy, biocompatibility of nanoparticles, cross-linking, antigen unmasking methods, antigen detection, immunohistochemistry, immunoassay.
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Faseroptische Gemischbildungsanalyse in Otto-Motoren bei direkteinspritzenden Brennverfahren / Fiberoptical analysis of the mixture formation process in gasoline direct injection combustion enginesThiele, Olaf 26 October 2004 (has links)
No description available.
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The significance of coherent flow structures for the turbulent mixing in wall-bounded flows / Die Bedeutung kohärenter Strukturen für die turbulente Vermischung in WandgrenzschichtenKähler, Christian Joachim 01 July 2004 (has links)
No description available.
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Simulationsrechnungen anisoplanatischer Übertragungsfunktionen für solare Adaptive Optik / Simulation of anisoplanatic transfer functions for solar Adaptive OpticsSailer, Markus Josef 03 August 2006 (has links)
No description available.
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Cavitation and shock wave effects on biological systems / Kavitation und Stoßwelleneffekte in biologischen SystemenWolfrum, Bernhard 10 February 2004 (has links)
No description available.
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Dielektrische Wellenleitergitter in Resonanz / Theorie, Charakterisierung und Anwendung / All-dielectric Resonant Waveguide Gratings / Theory, Characterization and ApplicationSelle, André 19 November 2008 (has links)
No description available.
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The Scanning Transmission X-Ray Microscope at BESSY II / Das Rasterröntgenmikroskop bei BESSY IIWiesemann, Urs 09 December 2003 (has links)
No description available.
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Multilagenbasierte Transmissionsoptiken für die Röntgenmikroskopie / Multilayer based transmission optics for x-ray microscopyLiese, Tobias 15 May 2012 (has links)
No description available.
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Mutual interactions of femtosecond pulses and transient gratings in nonlinear optical spectroscopyNolte, Stefan 16 November 2018 (has links)
This work is dedicated to a comprehensive experimental study on the interaction of femtosecond laser pulses with the nonlinear optical medium lithium niobate. The nonlinear optical response in the nanosecond regime was already studied extensively with a variety of techniques, whereas femtosecond pulses were mainly used in transient absorption or transient grating experiments. Naturally, the temporal resolution of these measurements depends on the pulse duration, however, dynamics during the pulse excitation were barely investigated.
The motivation of this work is to widen the limits of femtosecond spectroscopy, not only to temporally resolve faster nonlinear optical processes, but further to show a sensitivity to other coupling mechanisms between the pulses and the material. Especially, the role of transient, dynamic holographic gratings is investigated with a careful determination of the pulse duration, bandwidth and frequency chirp. A basis of this work is established in the first part by studying the material response via light-induced absorption before focusing on the main topic, the pulse interaction with elementary (holographic) gratings, both self-induced and static, in the second part. By this detailed study, several features of femtosecond laser pulses, holographic gratings and the ultrafast material response can be revealed: (i) grating recording is feasible even with pulses of different frequencies, provided that their pulse duration is sufficiently short, (ii) grating based pulse coupling causes a pronounced energy transfer even in a common pump-probe setup for transient absorption measurements with (non-)degenerated frequencies, (iii) beyond expectation, oscillations in the phonon frequency range become apparent in different measurements. The presented results point towards appropriate future experiments to obtain a more consistent, microscopic model for the ultrafast response of the crystal, involving the interplay between photo-generated polarons, self-induced gratings, and phonons.
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