The present study involves the determination of traces of thallium and mercury in aqueous solution by forming ion-association complexes of these metals with rhodamine 6G (R6G) in the presence of iodide. Thallium(lll) and mercury(Il) are able to quench the fluorescence of R6G in the presence of iodide by forming ion—association complexes, and the degree of quenching correlates with the thallium(III) or mercury(II) concentrations . By using this method, the minimum amount of thallium- (III) that can be determined in aqueous solutions is approximately 4.00 X 10-6m, while the minimum amount of mercury(II) is approximately 3.00 X 10-6m. Thallium(I) cannot be determined by this method because it is not possible to form ion-association complexes under similar experimental conditions therefore, this method can be used to differentiate between Tl(I) and Tl(III) in aqueous solution. To elucidate the composition of the ion-association complexes in aqueous solutions two spectrophotometric methods are used: the mole-ratio method and the continuous- variation method. The experimental results seem to indicate that two complexes are formed for the mercury(II)-R6G-iodide system. The empirical compositions of these complexes are tentatively determined to be [(R6G)HgI3] and [(R6G)2HgI4).

We address the problem of all-pairs shortest time communication of messages in futuregeneration 6G networks by modeling the highly dynamic characteristics of the network using a temporal graph. Based on this model, an elegant technique is proposed to devise an algorithm for finding the all-pairs shortest time paths in the temporal graph that can be used for all-pairs internodes communication of messages in the network. The proposed algorithm basically involves computations similar to only two matrix multiplication steps, once in the forward direction and then in the backward direction.

Future 6G wireless communications network will increase the data capacity to unprecedented numbers and thus empower the deployment of new real-time applications. Millimeter-Wave (mmWave) band and Massive MIMO are considered as two of the main pillars of 6G to handle the gigantic influx in data traffic and number of mobile users and IoT devices. The small wavelengths at these frequencies mean that more antenna elements can be placed in the same area. Thereby, high spatial processing gains are achievable that can theoretically compensate for the higher isotropic path loss. The propagation characteristics at mmWave band, create sparse channels in typical scenarios, where only few paths convey significant power. Considering this feature, Hybrid (analog-digital) Beamforming introduces a new signal processing framework which enables energy and cost-efficient implementation of massive MIMO with innovative smart arrays. In this setup, the analog beamalignment via beam selection in link access phase, is the critical performance limiting step. Considering the variable operating condition in mmWave channels, a desirable solution should have the following features: efficiency in training (limited coherence time, delay constraints), adaptivity to channel conditions (large SNR range) and robustness to realized channels (LOS, NLOS, Multipath, non-ideal beam patterns). For the link access task, we present a new energy-detection framework based on variable length channel measurements with (orthogonal) beam codebooks. The proposed beam selection technique denoted as composite M-ary Sequential Competition Test (SCT) solves the beam selection problem when knowledge about the SNR operating point is not available. It adaptively changes the test length when the SNR varies to achieve an essentially constant performance level. In addition, it is robust to non-ideal beam patterns and different types of the realized channel. Compared to the conventional fixed length energy-detection techniques, the SCT can increase the training efficiency up to two times while reducing the delay if the channel condition is good. Having the flexibility to allocate resources for channel measurements through different beams adaptively in time, we improve the SCT to eliminate unpromising beams from the remaining candidate set as soon as possible. In this way, the Sequential Competition and Elimination Test (SCET) significantly further reduces training time by increasing the efficiency. The developed ideas can be applied with different codebook types considered for practical applications. The reliable performance of the beam selection technique is evident through experimental evaluation done using the state-of-the-art test-bed developed at the Vodafone Chair that combines a Universal Software Radio Peripheral (USRP) based platform with mmWave frontends.

Investigation of tumor growth has traditionally been studied focusing only on the cancer cells. However, tumors consist of a complex tissue organization where heterotypic signaling occurs between different cell types. The cross-talk between tumor cells and other surrounding cell types may ultimately prove to be as important for the tumor cell behavior as the internal signaling cascades in the tumor cell itself.Myeloid cells, such as granulocytes and monocytes, and thrombocytes play an important role in the tumor tissue, as a tumor can be compared to a wound healing process without the normal regulation mechanisms. Platelets are thought to facilitate tumor cell extravasation by binding to the tumor cell and recruiting myeloid cells that secrete factors aiding tumor migration through the endothelial cells. Studying the content of granules and vesicles of the platelets and myeloid cells can provide important knowledge about how the tumor interactions are mediated and which key proteins that controls these processes.Serglycin is an intracellular proteoglycan that attaches chains of negatively charged glycosaminoglycans. It is thought to have a function in retaining and storing proteins in hematipoietic cells. In this project the impact of the loss of serglycin on platelets and myeloid cells was investigated, using a spontaneous insulinoma serglycin knockout mouse model. The results suggests that serglycin does not affect the amount of neutrophil granulocytes and monocytes in peripheral blood, nor does it seem to affect the amount of platelets sequestered to the tumor tissue. A co-staining for platelets and MMP9 positive granulocytes was also performed in order to assess if granulocyte-platelet interactions in the tumor were affected by loss of serglycin. Interactions between these cells were observed in both genotypes. Von Willebrand factor levels in the tumor tissue also remained unchanged upon loss of serglycin. However, preliminary experiments indicated that serglycin seems to play a role in the intracellular amounts of vimentin and VEGFB in undifferentiated primary bone marrow derived monocytes.

This diploma thesis was focused on study of barrier and transport properties of selected polyelectrolytes in hydrogel matrices by using diffusion techniques. The study of these properties was performed in horizontal diffusion cells where is observed the change in diffusion probe concentration over time. Diffusion experiments were performed on an agarose hydrogel with the addition of alginate, hyaluronic acid, polystyrene sulfonate, humic acids and as a model probe rhodamine 6G was used. Important parts of this thesis are also the methods which characterize the substances and hydrogel matrices such as rheology and potentiometric titration. The main aim of this diploma thesis was to investigate the effect of interactions between passing model dye (rhodamine 6G) and the appropriate gel (agarose + polyelectrolyte) on the fundamental diffusion parameters (effective diffusion coefficient, lag time, etc.).

Yes / As 5th Generation research reaches the twilight, the research community must go beyond 5G and look towards the 2030 connectivity landscape, namely 6G. In this context, this work takes a step towards the 6G vision by proposing a next generation communication platform, which aims to extend the rigid coverage area of fixed deployment networks by considering virtual mobile small cells (MSC) that are created on demand. Relying on emerging computing paradigms such as NFV (Network Function Virtualization) and SDN (Software Defined Networking), these cells can harness radio and networking capability locally reducing protocol signalling latency and overhead. These MSCs constitute an intelligent pool of networking resources that can collaborate to form a wireless network of MSCs providing a communication platform for localized, ubiquitous and reliable connectivity. The technology enablers for implementing the MSC concept are also addressed in terms of virtualization, lightweight wireless security, and energy efficient RF. The benefits of the MSC architecture towards reliable and efficient cell-offloading are demonstrated as a use-case. / This project has received funding from the European Union's H2020 research and innovation program under grant agreement H2020-MCSAITN- 2016-SECRET 722424 [2].

Driven by the surging need for seamless connectivity, research in the wireless communication area has dramatically evolved over the years to meet the increasing demand for data rate and seamless coverage. Such evolvement concurs with a notable increase in data traffic and the widespread of data-hungry devices, thereby inflicting stringent requirements on terrestrial networks. Despite the tremendous advances achieved through the past generations of wireless systems, almost half of the world's population remains unconnected, leading to an accentuated digital divide problem. Therefore, this work invigorates a new connectivity solution that integrates aerial and terrestrial communications with a high-altitude platform station (HAPS) to promote a sustainable connectivity landscape. The connectivity solution adopted in this thesis specifically integrates terrestrial base stations with hot-air balloons under the framework of a cloud-enabled HAPS via a data-sharing fronthauling strategy. The aerial (hot-air balloons) and terrestrial base stations, grouped into disjoint clusters, coordinate their mutual transmission to serve aerial (i.e., drones) and terrestrial users. This work studies the downlink communication from the cloud-enabled HAPS to the aerial and terrestrial users under practical system considerations, namely the limited transmit power and the limited-capacity fronthaul link, per-base station. To this end, the first part of the thesis devises a specific optimization problem that maximizes the network sum-rate while accounting for system design constraints to determine the user association strategy, i.e., user to terrestrial clusters or user to air clusters, and the associated beamforming vectors. The second part of the thesis, then, designs a different resource allocations optimization problem that accounts for the fairness among the users, thus adopting a proportionally fair scheduling scheme to assign users on frequency tones to maximize the log of the long-term average rate. On this account, the work solves a handful of non-convex intricate optimization problems using techniques from optimization theory, namely, fractional programming and $\ell_0$-norm approximation. The work consequently outlines the gains realized by providing on-demand coverage in crowded and unserved areas. Moreover, the thesis illustrates the benefits of coordinating the operations of aerial and terrestrial base stations for interference management, load-balancing, and fairness measures.

Im Mittelpunkt dieser Arbeit steht die selektive Funktionalisierung von Siliziumoxidnanostrukturen auf alkyl-passivierten Siliziumoberflächen welche durch rasterkraftmikroskopisch induzierte lokale anodische Oxidation (LAO) erzeugt werden. Bei der gezielten Immobilisierung von funktionalen Molekülen auf den Strukturen werden zwei verschiedene Routen verfolgt – Anbindung von ionischen Farbstoffen über elektrostatische Wechselwirkungen sowie stufenweise kovalente chemische Anbindung von bi-funktionalen Verbindermolekülen und Farbstoffen. Eine Untersuchung der hergestellten funktionalen Strukturen erfolgt mittels Rasterkraftmikroskopie, Raster-Kelvin-Mikroskopie sowie zeitaufgelöster Fluoreszenzmikroskopie und-spektroskopie. Durch zwei unabhängige Methoden kann gezeigt werden dass die Ladungen im lokalen Oxide vergleichsweise stabil sind und die elektrostatische Anbindung somit auch noch nach Tagen möglich sein sollte. Das Verhalten der elektrostatisch angebundenen Farbstoffe hängt stark von deren Art ab. Während es bei Rhodamin 6G nur zu einer minimalen spektralen Änderung im Vergleich zur Lösung kommt so zeigen spermin-funktionalisierte Perylenbisimidfarbstoffe eine deutliche H-Aggregation und Ausbildung von Excimerzuständen. Diese Zustände sind eindeutig thermisch aktiviert und zeigen eine wesentlich höhere Aktivierungsenergie als bei allen anderen bisher untersuchten Perylenaggregaten sowie eine Hysterese bei Temperaturveränderung. Die physikalische Ursache für dieses Phänomen liegt allem Anschein nach in der elektrostatischen Anbindung selbst welche ein instabiles Gleichgewicht mit der Wechselwirkung der Moleküle untereinander bildet. Eine geordnete kovalente Anbindung von funktionalen Silanmolekülen an die mittels LAO erzeugten Strukturen erfordert sehr definierte Prozessparameter. Die spektroskopische Untersuchung von an die funktionalen Silane chemisch angebundenen Fluoresceinfarbstoffen lässt indirekte Schlüsse auf deren Belegungsdichte und damit die Qualität der Silanmonolage zu.

Durch alternierende oder simultane Plasmapolymerisation und Farbstoffsublimation wurden Plasmapolymerschichten mit eingelagerten Farbstoffmolekülen hergestellt. Als Farbstoff wurde Rhodamin 6G verwendet. Die Absorptions- und Fluoreszenzcharakteristika der Schichten wurden untersucht und mit denen einfacher Farbstoffschichten verglichen. Dabei wurde die Menge der eingelagerten Farbstoffmoleküle variiert. Es zeigt sich, daß sich die Absorptionsbande des eingelagerten Farbstoffs bei den unterschiedlichen Schichten kaum verändert. Die Fluoreszenzspektren der Schichten mit wenigen eingelagerten Farbstoffmolekülen zeigen dasgleiche Verhalten wie Rhodamin 6G in Lösung. Mit steigender Farbstoffmenge wird die Fluoreszenzbande rotverschoben und es entsteht eine weitere Fluoreszenzbande. Diese wird mit Hilfe eines sequentiellen Energietransfers zu modifizierten Molekülen beschrieben. Mit Hilfe der zeitaufgelösten Fluoreszenzspektroskopie konnte der zeitliche Verlauf der Fluoreszenzintensität gemessen werden. Dabei ist der Fluoreszenzzerfall deutlich nicht-exponentiell. Die Zerfallskurven wurden mit einer gestreckten Exponentialfunktion angepaßt.

[ES] La estandarización de la Quinta Generación de redes móviles o 5G, ha concluido este año 2020. No obstante, en el año 2014 cuando la ITU empezó el proceso de estandarización IMT-2020, una de las principales interrogantes era cuál sería la forma de onda sobre la cual se construiría la capa física de esta nueva generación de tecnologías. El 3GPP se comprometió a entregar una tecnología candidata al proceso IMT-2020, y es así como dentro de este proceso de deliberación se presentaron varias formas de onda candidatas, las cuales fueron evaluadas en varios aspectos hasta que en el año 2016 el 3GPP tomó una decisión, continuar con CP-OFDM (utilizada en 4G) con numerología flexible. Una vez decidida la forma de onda, el proceso de estandarización continuó afinando la estructura de la trama, y todos los aspectos intrínsecos de la misma. Esta tesis acompañó y participó de todo este proceso. Para empezar, en esta disertación se evaluaron las principales formas de onda candidatas al 5G. Es así que se realizó un análisis teórico de cada forma de onda, destacando sus fortalezas y debilidades, tanto a nivel de implementación como de rendimiento. Posteriormente, se llevó a cabo una implementación real en una plataforma Software Defined Radio de tres de las formas de onda más prometedoras (CP-OFDM, UFMC y OQAM-FBMC), lo que permitió evaluar su rendimiento en términos de la tasa de error por bit, así como la complejidad de su implementación. Esta tesis ha propuesto también el uso de una solución armonizada como forma de onda para el 5G y sostiene que sigue siendo una opción viable para sistemas beyond 5G. Dado que ninguna de las forma de onda candidatas era capaz de cumplir por sí misma con todos los requisitos del 5G, en lugar de elegir una única forma de onda se propuso construir un transceptor que fuese capaz de construir todas las principales formas de onda candidatas (CP-OFDM, P-OFDM, UFMC, QAM-FBMC, OQAM-FBMC). Esto se consiguió identificando los bloques comunes entre las formas de onda, para luego integrarlos junto con el resto de bloques indispensables para cada forma de onda. La motivación para esta solución era tener una capa física que fuese capaz de cumplir con todos los aspectos del 5G, seleccionando siempre la mejor forma de onda según el escenario. Esta propuesta fue evaluada en términos de complejidad, y los resultados se compararon con la complejidad de cada forma de onda. La decisión de continuar con CP-OFDM con numerología flexible como forma de onda para el 5G se puede considerar también como una solución armonizada, ya que al cambiar el prefijo cíclico y el número de subportadoras, cambian también las prestaciones del sistema. En esta tesis se evaluaron todas las numerologías propuestas por el 3GPP sobre cada uno de los modelos de canal descritos para el 5G (y considerados válidos para sistemas beyond 5G), teniendo en cuenta factores como la movilidad de los equipos de usuario y la frecuencia de operación; para esto se utilizó un simulador de capa física del 3GPP, al que se hicieron las debidas adaptaciones con el fin de evaluar el rendimiento de las numerologías en términos de la tasa de error por bloque. Finalmente, se presenta un bosquejo de lo que podría llegar a ser la Sexta Generación de redes móviles o 6G, con el objetivo de entender las nuevas aplicaciones que podrían ser utilizadas en un futuro, así como sus necesidades. Completado el estudio llevado a cabo en esta tesis, se puede afirmar que como se propuso desde un principio la solución, tanto para el 5G como para beyond 5G, la solución es la armonización de las formas de onda. De los resultados obtenidos se puede corroborar que una solución armonizada permite alcanzar un ahorro computacional entre el 25-40% para el transmisor y del 15-25% para el receptor. Además, fue posible identificar qué numerología CP-OFDM es la más adecuada para cada escenario, lo que permitiría optimizar el diseño y despliegue de las redes 5G. Esto abriría la puerta a hacer lo mismo con el 6G, ya que en esta tesis se considera que será necesario abrir nuevamente el debate sobre cuál es la forma de onda adecuada para esta nueva generación de tecnologías, y se plantea que el camino a seguir es optar por una solución armonizada con distintas formas de onda, en lugar de solo una como sucede con el 5G. / [CA] L'estandardització de la Quinta Generació de xarxes mòbils o 5G, ha conclòs enguany 2020. No obstant això, l'any 2014 quan la ITU va començar el procés d'estandardització IMT-2020, uns dels principals interrogants era quina seria la forma d'onda sobre la qual es construiria la capa física d'esta nova generació de tecnologies. El 3GPP es va comprometre a entregar una tecnologia candidata al procés IMT-2020, i és així com dins d'este procés de deliberació es van presentar diverses formes d'onda candidates, les quals van ser avaluades en diversos aspectes fins que l'any 2016 el 3GPP va prendre una decisió, continuar amb CP-OFDM (utilitzada en 4G) amb numerología flexible. Una vegada decidida la forma d'onda, el procés d'estandardització va continuar afinant la frame structure (no se m'ocorre nom en espanyol), i tots els aspectes intrínsecs de la mateixa. Esta tesi va acompanyar i va participar de tot este procés. Per a començar, en esta dissertació es van avaluar les principals formes d'onda candidates al 5G. És així que es va realitzar una anàlisi teòrica de cada forma d'onda, destacant les seues fortaleses i debilitats, tant a nivell d'implementació com de rendiment. Posteriorment, es va dur a terme una implementació real en una plataforma Software Defined Radio de tres de les formes d'onda més prometedores (CP-OFDM, UFMC i OQAM-FBMC), la qual cosa va permetre avaluar el seu rendiment en termes de la taxa d'error per bit, així com la complexitat de la seua implementació. Esta tesi ha proposat també l'ús d'una solució harmonitzada com a forma d'onda per al 5G i sosté que continua sent una opció viable per a sistemes beyond 5G. Atés que cap de les forma d'onda candidates era capaç de complir per si mateixa amb tots els requeriments del 5G, en compte de triar una única forma d'onda es va proposar construir un transceptor que fóra capaç de construir totes les principals formes d'onda candidates (CP-OFDM, P-OFDM, UFMC, QAM-FBMC, OQAM-FBMC). Açò es va aconseguir identificant els blocs comuns entre les formes d'onda, per a després integrar-los junt amb la resta de blocs indispensables per a cada forma d'onda. La motivació per a esta solució era tindre una capa física que fóra capaç de complir amb tots els aspectes del 5G, seleccionant sempre la millor forma d'onda segons l'escenari. Esta proposta va ser avaluada en termes de complexitat, i els resultats es van comparar amb la complexitat de cada forma d'onda. La decisió de continuar amb CP-OFDM amb numerología flexible com a forma d'onda per al 5G es pot considerar també com una solució harmonitzada, ja que al canviar el prefix cíclic i el número de subportadores, canvien també les prestacions del sistema. En esta tesi es van avaluar totes les numerologías propostes pel 3GPP sobre cada un dels models de canal descrits per al 5G (i considerats vàlids per a sistemes beyond 5G), tenint en compte factors com la mobilitat dels equips d'usuari i la freqüència d'operació; per a açò es va utilitzar un simulador de capa física del 3GPP, a què es van fer les degudes adaptacions a fi d'avaluar el rendiment de les numerologías en termes de la taxa d'error per bloc. Finalment, es presenta un esbós del que podria arribar a ser la Sexta Generació de xarxes mòbils o 6G, amb l'objectiu d'entendre les noves aplicacions que podrien ser utilitzades en un futur, així com les seues necessitats. Completat l'estudi dut a terme en esta tesi, es pot afirmar que com es va proposar des d'un principi la solució, tant per al 5G com per a beyond 5G, la solució és l'harmonització de les formes d'onda. dels resultats obtinguts es pot corroborar que una solució harmonitzada permet aconseguir un estalvi computacional entre el 25-40% per al transmissor i del 15-25% per al receptor. A més, va ser possible identificar què numerología CP-OFDM és la més adequada per a cada escenari, la qual cosa permetria optimitzar el disseny i desplegament de les xarxes 5G. Açò obriria la porta a fer el mateix amb el 6G, ja que en esta tesi es considera que serà necessari obrir novament el debat sobre quina és la forma d’onda adequada per a esta nova generació de tecnologies, i es planteja que el camí que s’ha de seguir és optar per una solució harmonitzada amb distintes formes d’onda, en compte de només una com succeïx amb el 5G. / [EN] The standardization of the Fifth Generation of mobile networks or 5G is still ongoing, although the first releases of the standard were completed two years ago and several 5G networks are up and running in several countries around the globe. However, in 2014 when the ITU began the IMT-2020 standardization process, one of the main questions was which would be the waveform to be used on the physical layer of this new generation of technologies. The 3GPP committed to submit a candidate technology to the IMT-2020 process, and that is how within this deliberation process several candidate waveforms were presented. After a thorough evaluation regarding several aspects, in 2016 the 3GPP decided to continue with CP-OFDM (used in 4G) but including, as a novelty, the use of a flexible numerology. Once the waveform was decided, the standardization process continued to fine-tune the frame structure and all the intrinsic aspects of it. This thesis accompanied and participated in this entire process. To begin with, this dissertation evaluates the main 5G candidate waveforms. Therefore, a theoretical analysis of each waveform is carried out, highlighting its strengths and weaknesses, both at the implementation and performance levels. Subsequently, a real implementation on a Software Defined Radio platform of three of the most promising waveforms (CP-OFDM, UFMC, and OQAM-FBMC) is presented, which allows evaluating their performance in terms of bit error rate, as well as the complexity of its implementation. This thesis also proposes the use of a harmonized solution as a waveform for 5G and argues that it remains a viable option for systems beyond 5G. Since none of the candidate waveforms was capable of meeting on its own with all the requirements for 5G, instead of choosing a single waveform, this thesis proposes to build a transceiver capable of building all the main waveforms candidates (CP-OFDM, P-OFDM, UFMC, QAM-FBMC, OQAM-FBMC). This is achieved by identifying the common blocks between the waveforms and then integrating them with the rest of the essential blocks for each waveform. The motivation for this solution is to have a physical layer that is capable of complying with all aspects of beyond 5G technologies, always selecting the best waveform according to the scenario. This proposal is evaluated in terms of complexity, and the results are compared with the complexity of each waveform. The decision to continue with CP-OFDM with flexible numerology as a waveform for 5G can also be considered as a harmonized solution, since changing the cyclic prefix and the number of subcarriers, changes also the performance of the system. In this thesis, all the numerologies proposed by the 3GPP are evaluated on each of the channel models described for 5G (and considered valid for beyond 5G systems), taking into account factors such as the mobility of the user equipment and the operating frequency. For this, a 3GPP physical layer simulator is used, and proper adaptations are made in order to evaluate the performance of the numerologies in terms of the block error rate. Finally, a sketch of what could become the Sixth Generation of mobile networks or 6G is presented, with the aim of understanding the new applications that could be used in the future, as well as their needs. After the completion of the study carried out in this thesis, it can be said that, as stated from the beginning, for both 5G and beyond 5G systems, the solution is the waveform harmonization. From the results obtained, it can be corroborated that a harmonized solution allows achieving computational savings between 25-40% for the transmitter and 15-25% for the receiver. In addition, it is possible to identify which CP-OFDM numerology is the most appropriate for each scenario, which would allow optimizing the design and deployment of 5G networks. This would open the door to doing the same with 6G, i.e., a harmonized solution with different waveforms, instead of just one as in 5G. / Flores De Valgas Torres, FJ. (2020). Study on Air Interface Variants and their Harmonization for Beyond 5G Systems [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/164442 / TESIS