This study examined the effectiveness of social media marketing on college students attending a recreation special event. Facebook and Twitter were assessed via an experimental design. To measure the effectiveness of these applications (through updating statuses) on a college student’s awareness, interest, and intent of attending a special event, three groups were employed (Facebook, Twitter, and Control). A total of 134 participants were recruited via six undergraduate courses and were assigned into three groups based on their social media consumption. Subjects responded to a pre-survey, joined their respective treatment group, and completed a post-survey. Descriptive statistics revealed that awareness increased for those who received Facebook status updates and tweets. The interest level of the treatment groups decreased from the pre to the post-test and the intention of participants to participate increased slightly for the Facebook treatment group, but the intent level of participants in the Twitter and Control groups decreased.

Escherichia coli has a proven track record for successful production of anything from small molecules like organic acids to large therapeutic proteins, and has thus important applications in both R&amp;D and commercial production. The versatility of this organism in combination with the accumulated knowledge of its genome, metabolism and physiology, has allowed for development of specialty strains capable of performing very specific tasks, opening up opportunities within new areas. The work of this thesis has been devoted to alter membrane transport proteins and the regulation of these, in order for E. coli to find further application within two such important areas. The first area was vaccine development, where it was investigated if E. coli could be a natural vehicle for live vaccine production. The hypothesis was that the introduction and manipulation of a protein surface translocation system from pathogenic E. coli would result in stable expression levels of Salmonella subunit antigens on the surface of laboratory E. coli. While different antigen combinations were successfully expressed on the surface of E. coli, larger proteins were affected by proteolysis, which manipulation of cultivation conditions could reduce, but not eliminate completely. The surface expressed antigens were further capable of inducing proinflammatory responses in epithelial cells. The second area was biorefining. By altering the regulation of sugar assimilation, it was hypothesized that simultaneous uptake of the sugars present in lignocellulose hydrolyzates could be achieved, thereby improving the yield and productivity of important bio-based chemicals. The dual-layered catabolite repression was identified and successfully removed in the engineered E. coli, and the compound (R)-3-hydroxybutyric acid was produced from simultaneous assimilation of glucose, xylose and arabinose. / <p>QC 20150508</p>

Thesis (M.A.)--Baylor University, 2009. / Includes bibliographical references (p. 155-161).

Vår studie belyser hur informationsspridning både muntligt och via internet kan påverka turisters reseplaneringsprocess. Studien undersöker vilka känslomässiga attityder individer upplever genom word of mouth och electronic word of mouth och hur vida dessa attityder påverkar valet av ett nytt resmål. Syftet med studien är att undersöka hur word of mouth kan påverka turisters val av nya resmål. För att möjliggöra detta har vi genomfört intervjuer med respondenter utifrån förutbestämda kriterier: personer som reser, använder sociala medier och är mellan 20-60 år gamla. Vi lyfter fram huvudbegreppet word of mouth som det finns mycket forskning kring där även ett nytt begrepp har framkommit, electronic word of mouth då internetutvecklingen bidrog till att sociala medier har blivit en ny plattform för information. Genom dessa begrepp lyfts fenomenet fram och genom begreppen turistblicken och reseplaneringsprocessen förklaras människans beteende som turist. Vår studie visar att respondenterna blir påverkade av word of mouth och eletronic word of mouth. Resultatet av studien konstaterar att word of mouth är en informationskälla och electronic word of mouth är en inspirationskälla som båda upplevs som en viktig del i reseplaneringsprocessen. Majoriteten av respondenterna har rest till ett nytt resmål utifrån electronic word of mouth och anser att word of mouth är ett viktigt komplement i reseplaneringsprocessen.

With the help of various advertising strategies this study addresses the Swedish, plant-basedfood-production company Oatly, and their advertisements to see how the discourse onsustainability is approached. By using critical discourse analysis, and primarily Fairclough’sthree-dimensional-model for analysing discourse (1989, 1995) as well as the marketingframework AIDA, these advertisements have been analysed to see how the companymanages to tempt and persuade their consumers into consumption. This paper seeks tounderstand how Oatly portrays their products as the “right” choice, by acting on and creatingsocial, public understandings. But who decides what is “correct” and what is not, and howdoes a company act on contemporary social conventions to portray themselves as the “good”choice? Through a textual analysis of Oatly’s product descriptions on their website as well asof the product packaging in-store, this report has established that Oatly acts on publicunderstandings of environmental sustainability to persuade their audience into consumption.

The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. One of the more commonly used autotransporters is the Adhesin Involved in Diffuse Adherence (AIDA) of pathogenic Escherichia coli. The surface expression of enzymes and vaccine epitopes offer several advantages. Surface expressed enzymes gain similar properties to immobilised enzymes, mainly simplified handling and separation using centrifugation. Surface expressed vaccine epitopes can have longer half-lives inside the animal that is to be immunized and surface groups on the host cell can act as adjuvants, increasing the immune response and leading to a better immunisation.    However, while much basic research is directed towards mechanisms of surface expression using autotransporters there are few reports regarding production of surface expressed protein. Thus the aim of this work was the optimisation of the yield and productivity of surface expressed protein. Protein Z, an IgG-binding domain of Staphylococcal protein A, was used as a model protein for the investigation of which cultivation parameters influenced surface expression. The choice of cultivation medium gave the largest impact on expression, which was attributed to effects based on the induction of the native promoter of AIDA. The AIDA system was then used for the expression of two Salmonella surface proteins, SefA and H:gm, with potential for use as vaccine epitopes. SefA was verified located on the cell surface, and H:gm was found in the outer membrane of the host cell, though only in proteolytically truncated forms lacking the His6-tag used for detection. This proteolysis persisted in E. coli strains deficient for the outer membrane protease OmpT and was concluded to be dependent on other proteases. The removal of proteolysis and further optimisation of the yield of surface-expressed protein are important goals of further work. / QC 20111123 / Vinnova: BIO-AMINES / SIDA Vietnam: Production of viral proteins for vaccine development

Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. In this thesis, a modular surface expression system, utilizing dual tag detection, was therefore created. It was based on the adhesin involved in diffuse adherence (AIDA-I) autotransporter, and was here used to express the model proteins SefA and H:gm on the cell surface of Escherichia coli. Due to the dual tag detection system, proteolysed H:gm could be successfully verified on the cell surface. By optimizing cultivation conditions, surface expression yield of SefA was increased by 300 %, and proteolysis reduced by 33 %. While proteolysis could not be eliminated completely, the work presented in this thesis is a major step towards a general system for surface expression of a wide range of proteins in varied applications. / <p>QC 20130506</p>

The purpose of the study is to investigate how men and women experience personalized advertising, whether it affects the purchase decision and whether there are differences between men and women. A qualitative method has been used with the help of structured interviews within a delimitation of the Stockholm area. The theories used in this study consisted of the AIDA model, Customer Relationship Management (CRM), Relationship Marketing (RM), Customization and Personalization and Consumer Culture Theory. Results of the study show that there are clear differences in how men and women experience Instagram. The main difference is that women have a more positive view of personalized advertising, which leads to actual purchases to a greater extent than men. The men in the study, on the other hand, show that it is negative to individualized advertising to a greater extent.

This master's thesis explores the impact of marketing communication tools on consumer behavior in the Russian alcohol market. Russia is a dark market. The dark market is a market where outdoor advertising is prohibited, as well as advertising on TV and radio. As such, communications with consumers in these markets are strongly regulated by the government. The study provides an overview of current trends in the alcohol market in the world and in Russia. The purpose of this study is to analyse Russian consumers' perceptions of marketing tools and the impact of these promotion tools on the purchase of strong alcohol under the AIDA model. For this purpose, the study examines the legislation governing the Russian alcohol market. The paper provides a description and classification of channels and instruments used in communications with consumers in the dark market conditions. Further, the perception of these methods by Russian consumers under the AIDA model is considered. The study identified the most effective channels of communication for marketing companies. Moreover, the ethical aspect of promoting alcohol products was discussed. The survey results showed that Russian consumers are loyal to both alcohol advertising and the market in general, as well as to their own brands. These results can be used by companies to create marketing campaigns and plan their budgets.

I denna studie analyseras filmen Quo Vadis, Aida? (2020) utifrån dess potential att utveckla narrativ kompetens, som ett uttryck för historiemedvetande, hos elever i en undervisningskontext. Filmen analyseras utifrån en narrativ analysmetod med fokus på vad filmen berättar samt hur det berättas. Därefter används Rüsens teoretiska begrepp erfarandets, tolkandets samt orienterandets kompetens för att belysa filmens potential att utveckla narrativ kompetens. Rüsens teoretiska begrepp används som utgångspunkt men vävs också ihop med fler teoretiska analysverktyg, som den dubbla tankeoperationen och historisk empati. Utifrån tidigare forskning diskuteras sedan filmens möjligheter samt utmaningar att användas i historieundervisningen i den svenska gymnasieskolan. Resultatet visar att det finns potential för Quo Vadis, Aida? att kunna användas på ett meningsfullt sätt i historieundervisningen för att utveckla elevers narrativa kompetens, som uttryck för historiemedvetande. Möjligheterna utgörs av filmens sätt att gestalta och väva samman ”små” och ”stora” berättelser, dess potential att beröra eleverna känslomässigt, samt öppna upp för värdeladdade frågor och diskussioner kring moraliska dilemman. Det finns också ett par utmaningar som behöver tas i beaktande för att filmen ska kunna leva upp till sin fulla potential. Det är ett tidkrävande och komplext arbete för läraren att se till att det finns en balans mellan känslor och kognitiva processer i undervisningen, för att inte elevernas känslor ska ta över och riskera att utvecklandet av historisk empati och historiemedvetandet blir lidande.