In the last two decades or so, hybrid structures from dissimilar materials and/or sheet gauges have been developed to achieve weight reduction while maintaining or even improving structural performance such as stiffness, crash and impact behavior. In particular, welded and brazed sheet materials in the form of tailor blanks (TBs) are being increasingly used or considered for future applications in different applications such as automotive, aerospace and marine constructions as they offer attractive combination of strength and performance in applications where weight reduction is desirable. However, technical problems are often encountered during forming of TBs from dissimilar base sheet materials with different thickness and/or strength. These include weld line movement and non-uniform deformation. Additionally, there are premature weld failures due to the presence of softening zone (as in TBs made from advanced high strength steels), and brazed interface failure due to insufficient bonding and wetting (as in TBs made from steel and aluminum). These areas of forming of TBs need to be scientifically studied to advance the use of dissimilar materials. The current research involves an understanding of deformation and forming behavior of steel-to-steel tailor welded blanks (TWBs) made from advanced high strength steel (AHSS) such as dual phase (DP780) steel. The research also involves a study of deformation behavior of steel-to-aluminum tailor brazed blanks (TBBs). TWBs have been successfully joined using a relatively new welding techniques such as defocused fiber laser welding. TBBs, on the other hand, have been successfully produced by fiber Laser/MIG hybrid brazing and Cold Metal Transfer brazing (CMT). In addition, the formability of TWBs of different gauges and/or strengths was tested by using a new, simple and reproducible method of formability testing using a double-layer blank method. This method was devised and assessed for testing various steel combinations in different strain paths such that the weld line stayed in position with respect to forming tools and is subjected to the same stress and strain state as the parent material in the weld and its vicinity. Moreover, results from conventional stretch forming tests, single-layer blank, and the double-layer method were compared at the macroscopic level (such as weld line movement, forming limit etc.) as well as at the microscopic level (such as failure location within the weld and failure mode) to isolate the advantages of the proposed double-layer method. With regard to TBBs made by fiber Laser/MIG and CMT brazing methods, a fundamental knowledge and understanding of the local deformation behavior and material plastic ow in and around the brazed steel-aluminum interfaces were obtained by conducting miniature tensile mechanical tests that focus on continuous observation of the brazed region under a high magnification optical microscope to assess the ductility of the brazed joint and its capacity to carry the load during a material shaping process. / Thesis / Doctor of Philosophy (PhD) / In the last two decades or so, hybrid structures from dissimilar materials and/or sheet gauges have been developed to achieve weight reduction while maintaining or even improving structural performance such as stiffness, crash and impact behavior. In particular, welded and brazed sheet materials in the form of tailor blanks (TBs) are being increasingly used or considered for future applications in different applications such as automotive, aerospace and marine constructions as they offer attractive combination of strength and performance in applications where weight reduction is desirable. However, technical problems are often encountered during forming of TBs from dissimilar base sheet materials with different thickness and/or strength. These include weld line movement and non-uniform deformation. Additionally, there are premature weld failures due to the presence of softening zone (as in TBs made from advanced high strength steels), and brazed interface failure due to insufficient bonding and wetting (as in TBs made from steel and aluminum). These areas of forming of TBs need to be scientifically studied to advance the use of dissimilar materials.

Cedar, represented by Western redcedar (Thuja plicata) and Yellow Cedar (Chamaecyparis nootkatensis) was known as the “Tree of Life” to the Nuu-chah-nulth on Vancouver Island’s west coast, and most other groups of the Pacific Northwest. This thesis investigates the Culturally Modified Trees (CMTs), or more specifically Tapered Bark Strips (TBS), created through the extraction of cedar bark removed for all manner of material goods. CMTs are now the most common archaeological site type within British Columbia. Current regional chronologies have inherent biases that make interpretations difficult. The chronologies created through Archaeological Impact Assessments (AIAs) are weighted heavily to the contact period and the highest frequency of use corresponds with indigenous population collapse rather than peak. Investigations are made into the true distribution of existing CMT features. This thesis details the survey of 16 recent old growth cedar clearcuts which found extensive unrecorded CMT features that have recently been logged throughout the southern Nuu-chah-nulth study region. Half of all TBS scars in exposed stumps were found embedded within healed trees, otherwise invisible to archaeologists. Comparing all AIA report dates (surveyed prior to logging activity) with all post-impact assessments surveys it was found the latter contain a greater and older distribution of scarring events corresponding to high First Nations populations before the contact period. The study also compares CMT chronologies with local histories, investigates the antiquity of Northwest Coast CMTs and the indigenous management of cedar trees to maximize bark harvests. The findings of this research hint at the expanded extent of anthropogenic forests in the Northwest Coast, the inadequate recording and heritage protections of CMTs, and what it all means for Aboriginal Land Rights in British Columbia. / Graduate / 0324 0740 0329 / kinze.earnshaw@gmail.com

THESIS ABSTRACT Name: Bc. Lenka Košnarová Supervisor: Mária Krivošíková, M.Sc. Opponent: Title of thesis: Memory disorders assessment in patients with Alzheimer's disease Abstract: Objective: The main objective of the thesis was to investigate the impact of memory disorders on self-care in patients with Alzheimer's disease (AD). Another objective of the thesis was to determine whether age of patients with AD affects occupational performance in daily living activities. Methods: The sample was consist of 50 probands (11 men, 39 women), who were diagnosed AD by physician. For data collection was used a questionnaire Bristol Activities of Daily Living scale (BADLS) evaluating self-care and Contextual Memory Test (CMT) for evaluation of memory disorders. Testing the hypothesis was performed by correlation the corrected Spearman correlation coefficient and regression analysis. Results: The thesis results confirmed a significant correlation between BADLS scores and CMT scores - immediate recall (rSp = .51; p<0.05), delay recall (rSp = .42; p<0.05), total (rSp = .48; p<0.05). Furthermore, research results suggest that memory impairment occur more with PADLS. There also was no statistically significant relationship between age and performance of activities of daily living (ADL) coefficient (rSp = .07; p<0.05)....

As Neoplasias endócrinas múltiplas (NEMs) são síndromes herdadas de modo dominante e causadas por mutações germinativas em genes específicos. Caracterizam-se pela presença de tumores em um conjunto de glândulas endócrinas, conjunto este típico de cada tipo-específico de NEM. Dentre os diferentes tipos de NEMs, há a neoplasia endócrina múltipla tipo 2 (NEM2) que envolve os fenótipos, carcinoma medular de tireóide (CMT), hiperparatiroidismo primário (HPT), feocromocitoma (FEO) e megacólon congênito (doença de Hirschsprung, HSCR). Apesar da prevalência da NEM2 na população em geral ser baixa (~ 1:30.000), o número de casos afetados por família pode ser expressivo, uma vez que sua penetrância é praticamente completa (~100%). A doença de HSCR ou aganglionose intestinal congênita quando ocorre está geralmente associada à mutações RET nos códons 609, 618 e 620; apresenta ampla variação fenotípica, padrão de herança complexa e baixa penetrancia. Poucos casos de HSCR podem apresentar mutações em outros genes. Mutações no gene RET são responsáveis por aproximadamente metade (~50%) dos casos familiares de HSCR e alguns casos esporádicos (~10-20%), sugerindo fortemente que a HSCR seja doença poligenica. Tem-se também sugerido que polimorfismos genéticos no RET podem influenciar o fenotipo da NEM2/HSCR. No presente estudo, analisamos o gene RET no sentido de investigar se o desenvolvimento de megacólon em pacientes com a mutação germinativa RET p.C620R estaria associado à presença de ou a) a uma segunda mutação germinativa ou b) a um SNP, ou c) a um haplótipo informativo, que possivelmente poderia estar potencialmente interagindo genicamente com a mutação RET principal e eventualmente modulando o fenótipo HSCR / The multiple endocrine neoplasias (MENs) are inherited multi-tumoral conditions caused by germline mutations in specific genes. Specifically, the multiple endocrine neoplasia type 2 (NEM2) is a hereditary endocrine disorder transmitted dominantly and involving three main tumors, medullary thyroid carcinoma (CMT), primary hyperparathyroidism (HPT) and pheochromocytoma (PHEO). Despite the low prevalence of MEN2 in general population, the number of affected individuals per family can be significant as the penetrance of MEN2 is almost complete (~100%). In addition to CMT, PHEO and HPT, other conditions as congenital megacólon (Hirschsprung disease, HSCR or congenital intestinal aganglionosis) may occur in MEN2 (HSCR/MEN2). HSCR/MEN2 usually is due to RET mutations in codons 609, 618 and 620. HSCR has a wide phenotypic variation; is a complex multigenic disease; and has a low penetrance. Mutations in the RET gene are responsible for approximately 50% of the familial HSCR cases and ~10% of the sporadic HSCR cases, supporting that HSCR is a polygenic disease and this is confirmed by a few HSCR cases associated with mutations in the EDNRB and EDN3 genes. In the present study, we focused in the analysis of the RET gene in order to investigate whether the development of congenital megacólon in patients with RET mutation p.C620R is associated with the presence of, a) a second RET germline mutation, b) a SNP, or with a haplotype that co-segregate with the disease

Η παρούσα διπλωματική εργασία αποτελεί μια προσπάθεια διερεύνησης της Συναισθηματικής Νοημοσύνης, της Ηγεσίας και της Διοίκησης Συγκρούσεων σε Ομάδες Διαχείρισης Κρίσεων (ΟΔΚ) και πώς όλα τα παραπάνω διαμορφώνονται με βάση τη χρονική στιγμή σε σχέση με την κρίση, δηλαδή τι ισχύει πριν, κατά τη διάρκεια και μετά την κρίση. Στην εμπειρική έρευνα που πραγματοποιήθηκε χρησιμοποιήθηκαν 117 δομημένα ερωτηματολόγια τα οποία συμπληρώθηκαν από στελέχη που είχαν συμμετάσχει τουλάχιστον μια φορά σε ΟΔΚ. Tο μεγαλύτερο ποσοστό των συμμετεχόντων στην έρευνα προέρχεται από τα Σώματα Ασφαλείας (Αστυνομία, Πυροσβεστική, Λιμενικό). Επίσης συμμετείχαν μέλη ΟΔΚ από Υπουργεία, Οργανισμούς του Ευρύτερου Δημοσίου Τομέα και Ένοπλες Δυνάμεις. Η παρούσα έρευνα οδήγησε σε ενδιαφέροντα συμπεράσματα, αναδεικνύοντας τους παράγοντες εκείνους που επηρεάζουν σημαντικά τη διαχείριση κρίσεων από τις εργασιακές ομάδες. Η διαχείριση κρίσεων αποτελεί μια δυναμική διαδικασία αλληλένδετων φάσεων, όπου η μια επηρεάζει την άλλη σημαντικά, καθορίζοντας σε μεγάλο βαθμό την εξέλιξή της. Παρατηρούμε λοιπόν ότι όταν η κάθε οργάνωση έχει την ικανότητα να εντοπίσει έγκαιρα τα σημάδια της επερχόμενης κρίσης, αυτό οδηγεί στην καλύτερη αντιμετώπισή της κατά τη δεύτερη φάση και εν συνεχεία όσο πιο αποτελεσματική είναι η αντιμετώπιση κατά τη δεύτερη φάση τόσο πιο γρηγορα θα επιστρέψει ο οργανισμός σε ομαλή λειτουργία. Συγκεκριμένα εξετάζοντας τη φάση πριν την κρίση καταλήγουμε στο συμπέρασμα ότι η φάση αυτή εξαρτάται σημαντικά από την κατηγορία της κρίσης που ξεσπά στην οργάνωση. Oι δυσεπίλυτες κρίσεις επηρεάζουν σε πολύ μεγάλο βαθμό την ανίχνευση, την πρόληψη και την προετοιμασία για την αντιμετώπιση τους, δηλαδή τη φάση πριν την κρίση, ενώ με ένα μικρότερο ποσοστό ακολουθούν οι συνηθισμένες κρίσεις. Έτσι καταλήγουμε στο ότι αυτοί οι τύποι κρίσεων διευκολύνουν τη Δημόσια Διοίκηση να είναι σε θέση να αναγνωρίζει πιθανά σημάδια έλευσής τους, να είναι προετοιμασμένη και να έχει την ικανότητα να τις αποτρέψει εν τη γενέσει. Σε ότι αφορά την αυτοεπίγνωση ως μερος της συναισθηματικής νοημοσύνης του επικεφαλής της ομάδας, θα μπορούσαμε να πούμε ότι με το να έχει ο τελευταίος αυτοεπίγνωση και επίσης να διατηρεί τον έλεγχο των αντιδράσεών του είναι δυνατό να προετοιμάσει την ομάδα του καλύτερα για μια κατάσταση κρίσης. Οι συνηθισμένες κρίσεις όπως και στην πρώτη φάση έτσι και κατά τη διάρκεια της κρίσης επηρεάζουν σημαντικά την αντιμετώπισή της. Επιπρόσθετα οι απρόσμενες κρίσεις φαίνεται να επηρεάζουν αρνητικά την αντιμετώπιση μιας κρίσης, προκαλώντας προβλήματα κατά τη φάση της κρίσης. Επίσης η επικοινωνία του επικεφαλής με τα μέλη της ομάδας του μέσα κυρίως από την ενσυναίσθηση και τις κοινωνικές του δεξιότητες και η ικανότητα του να λαμβάνει γρήγορες αποφάσεις στη διάρκεια της κρίσης συμβάλλει στην συνεργασία. Όσο καλύτερα οι ομάδες διαχειρίζονται τις κρίσεις, τόσο θα διασφαλίζεται η αποκατάσταση της ομαλότητας του οργανισμού και εν συνεχεία η αποκόμιση γνώσης και εμπειρίας. Η συναισθηματική νοημοσύνη και η επικοινωνία του ηγέτη επιδρά θετικά τόσο στην οργανωσιακή μάθηση, όσο και στη διαχείριση του άγχους των μελών της ομάδας. Αναφορικά με τη διοίκηση συγκρούσεων στο πλαίσιο της ομάδας αποδεικνύεται ότι η επιλογή της ενσωμάτωσης ή του συμβιβασμού επηρεάζεται σημαντικά από την ικανότητα των μελών της ομάδας να λαμβάνουν αποφάσεις. Όταν η ομάδα είναι προετοιμασμένη σωστά και έχει την ικανότητα να εντοπίσει έγκαιρα τα σημάδια της επερχόμενης κρίσης, αυτό οδηγεί στην επιλογή από το μέλος της ομάδας της εναρμόνισης με τον επικεφαλής ή έστω του συμβιβασμού για τη διαχείριση διαφωνιών. Η ανεπίσημη επικοινωνία μεταξύ των μελών της ομάδας επηρεάζει σε μικρό βαθμό αλλά αρνητικά τον συμβιβασμό μεταξύ των μελών της ομάδας και του επικεφαλής μιας και η προφορική επικοινωνία ή η καταγεγραμμένη ενημέρωση δημιουργούν πολλές φορές εντάσεις και τα άτομα δεν είναι διατεθειμένα να «μοιράσουν τη διαφορά» και να αναλάβουν ευθύνη φτάνοντας σε κοινές αποφάσεις. Σχευικά με τους παράγοντες εκείνους που επηρεάζουν σημαντικά τη διαχείριση κρίσεων δεν συναντήσαμε την αποτελεσματική ηγεσία του επικεφαλής μέσα από την ικανότητα του να εκτιμά πληροφορίες και να λαμβάνει αποφάσεις σε συνθήκες καθημερινής πίεσης και υψηλού ρίσκου καθώς και την βαθύτερη ανάπτυξη της συναισθηματικής του νοημοσύνης σχετικά με τα μέλη της ομάδας. Αυτό συνδέεται σε ορισμένες περιπτώσεις με την έλλειψη ή/και τοποθέτηση μη κατάλληλων στελεχών ικανών να ηγηθούν ομάδων σε συνθήκες κρίσης. Η αναπολεσματική διοίκηση σε πολλές περιπτώσεις ειδικά σε θέματα οργανωσιακών αλλαγών (δομές, διαδικασίες) ενισχύεται από την οργανωσιακή κουλτούρα καθώς και τους περιορισμούς από το πολιτικό και οικονομικό περιβάλλον (κεντρική διακυβέρνηση, μνημόνια). / This study aims to investigate the relationships between Emotional Intelligence, Leadership and Conflict Management in Crisis Management Teams (CMT). Participants were 117 individulas involved in CMT working at various fuctional units and different hierarchical levels in Police Force, Fire Service, Public Health Care organizations, Ministries and Army forces. This study contributes to our understanding of the defining factors that lead to successful crisis handling by teams. Crisis Management is a dynamic process that consists of three related stages: pre crisis, crisis and post crisis stage. Results provide support for the model since signal detection and preparation leads organization to a better response during crisis stage and subsequently to recovery in post crisis stage. Predictability of the crisis (intractable, conventional crises) is positively associated with handling during pre crisis stage. Also, leaders’ intrapersonal competencies (self awareness, self regulation) help them in order to face a crisis situation effectively on team basis. Research findings indicate that conventional crises are also related positively with appropriate handling during crisis stage. On the other hand, unexpected crises have a negative impact on crisis management process since public administration is not well prepared for the prescribed type of crisis. Overall leader-team member communication using interpersonal competencies (empathy, social skills) contributes effectively to cooperation. Team leader’s communication with members also enhance organizational learning during post crisis stage. On another but related issue crisis management is a group decision-making process. Results suggest that team members’ ability to make decisions is associated positively with the utilization of integrating or comprosing as conflict management style. Especially when CMT follows signal detection, prevention and crisis preparation, members have the willingness to integrate goals or to compromise with supervisor in order to reach a positive outcome for teamwork. Crisis leadership is another crucial factor for effective handling. According to empirical findings CMT leader’s ability in public administration to assess information and make decisions in the face of ambiguity, high stakes, and urgency is limited. Poor management in some cases maybe is due to internal factors (lack of human resources, selection process, organizational culture) as well as external factors (political leadership, economic crisis).

Title: Numerical modeling of free oscillations applied to superconducting-gravimeter data in a low-frequency seismic range Author: Eliška Zábranová Department: Department of Geophysics Supervisor: Doc. RNDr. Ctirad Matyska, DrSc. Abstract: Deformations and changes of the gravitational potential of prestressed selfgravitating elastic bodies caused by free oscillations are described by means of the momentum and Poisson equations and the constitutive relation. For spheri- cally symmetric bodies we transform the equations and boundary conditions into ordinary differential equations of the second order by the spherical harmonic de- composition and further discretize the equations by highly accurate pseudospectral difference schemes on Chebyshev grids. We thus receive a series of matrix eigenvalue problems for eigenfrequencies and eigenfunctions of the free oscillations. Since elas- tic parameters are frequency dependent, we solve the problem for several fiducial frequencies and interpolate the results. Both the mode frequencies and the eigen- functions are benchmarked against the output from the Mineos software package based on Runge-Kutta integration techniques. Subsequently, we use our method to calculate low-frequency synthetic accelerograms of the recent megathrust events and compare them with the observed...

As Neoplasias endócrinas múltiplas (NEMs) são síndromes herdadas de modo dominante e causadas por mutações germinativas em genes específicos. Caracterizam-se pela presença de tumores em um conjunto de glândulas endócrinas, conjunto este típico de cada tipo-específico de NEM. Dentre os diferentes tipos de NEMs, há a neoplasia endócrina múltipla tipo 2 (NEM2) que envolve os fenótipos, carcinoma medular de tireóide (CMT), hiperparatiroidismo primário (HPT), feocromocitoma (FEO) e megacólon congênito (doença de Hirschsprung, HSCR). Apesar da prevalência da NEM2 na população em geral ser baixa (~ 1:30.000), o número de casos afetados por família pode ser expressivo, uma vez que sua penetrância é praticamente completa (~100%). A doença de HSCR ou aganglionose intestinal congênita quando ocorre está geralmente associada à mutações RET nos códons 609, 618 e 620; apresenta ampla variação fenotípica, padrão de herança complexa e baixa penetrancia. Poucos casos de HSCR podem apresentar mutações em outros genes. Mutações no gene RET são responsáveis por aproximadamente metade (~50%) dos casos familiares de HSCR e alguns casos esporádicos (~10-20%), sugerindo fortemente que a HSCR seja doença poligenica. Tem-se também sugerido que polimorfismos genéticos no RET podem influenciar o fenotipo da NEM2/HSCR. No presente estudo, analisamos o gene RET no sentido de investigar se o desenvolvimento de megacólon em pacientes com a mutação germinativa RET p.C620R estaria associado à presença de ou a) a uma segunda mutação germinativa ou b) a um SNP, ou c) a um haplótipo informativo, que possivelmente poderia estar potencialmente interagindo genicamente com a mutação RET principal e eventualmente modulando o fenótipo HSCR / The multiple endocrine neoplasias (MENs) are inherited multi-tumoral conditions caused by germline mutations in specific genes. Specifically, the multiple endocrine neoplasia type 2 (NEM2) is a hereditary endocrine disorder transmitted dominantly and involving three main tumors, medullary thyroid carcinoma (CMT), primary hyperparathyroidism (HPT) and pheochromocytoma (PHEO). Despite the low prevalence of MEN2 in general population, the number of affected individuals per family can be significant as the penetrance of MEN2 is almost complete (~100%). In addition to CMT, PHEO and HPT, other conditions as congenital megacólon (Hirschsprung disease, HSCR or congenital intestinal aganglionosis) may occur in MEN2 (HSCR/MEN2). HSCR/MEN2 usually is due to RET mutations in codons 609, 618 and 620. HSCR has a wide phenotypic variation; is a complex multigenic disease; and has a low penetrance. Mutations in the RET gene are responsible for approximately 50% of the familial HSCR cases and ~10% of the sporadic HSCR cases, supporting that HSCR is a polygenic disease and this is confirmed by a few HSCR cases associated with mutations in the EDNRB and EDN3 genes. In the present study, we focused in the analysis of the RET gene in order to investigate whether the development of congenital megacólon in patients with RET mutation p.C620R is associated with the presence of, a) a second RET germline mutation, b) a SNP, or with a haplotype that co-segregate with the disease

This diploma thesis deals with the structural design and production of a oscillating head for surface welding of a protective layer of Inconel® 625 superalloy on membrane walls. The design is preceded by a research for possible variants of the solution. Of the two designs, one was successfully manufactured and assembled, and incentives for future improvements were set. For easy optimization and testing of the device, an oscillating motion program was created in the LinMoT Talk 6.9 software. The production costs for the manufactured equipment were then calculated and the recommended selling price was determined, based on a general calculation formula. The result of the project is a functional device, a proposal for its control and a technical - economic evaluation of the production process.

No description available.

A partir de 280 isolados fúngicos de cana-de-açúcar, 18 cepas foram avaliadas quanto á presença de genes da policetídeo sintase por meio da técnica do PCR. Estes fungos foram identificados taxonomicamente por uma abordagem polifásica, classificando-os dentro de quatro ordens e nove gêneros. A avaliação da atividade biológica demonstrou a presença de metabolitos com propriedades antibióticas quando enfrentados a micro-organismos patogênicos. Segundo a análise de correspondência múltipla, esta atividade poderia estar associada com a local de isolamento dos fungos. Foram detectadas 36 seqüências similares a genes PKS a partir de 17 destes fungos. A análise filogenética do domínio KS, conduzida pelo método de neighbor-joining, indicou que 16 seqüências se acomodaram dentro do grupo monofilético dos PKS envolvidos na produção de policetídeos não reduzidos e as outras 10 seqüências se acomodaram dentro do grupo monofilético dos PKS envolvidos na produção de policetídeos reduzidos. A análise do domínio CMT também apontou que as seqüências podiam se acomodaram em grupos de PKS dependendo do grau de redução do policetídeo, todas as seqüências CMT se relacionaram com PKS envolvidos na produção de policetídeos reduzidos. As análises dos modelos estruturais também demonstraram que as seqüências estavam altamente relacionadas com estruturas protéicas da família das enzimas de condensação, destacando a presença de uma hélice característica que carrega o resíduo de cisteína, responsável pela atividade de condensação. Extratos orgânicos obtidos de cultivos dos fungos foram avaliados parar detectar a presença de compostos tipo lovastatina. Por meio de cromatografia CCDS, detectaram-se bandas de 10 extratos com o mesmo deslocamento que a lovastatina padrão, mas apenas 6 destas foram confirmados por CLAE. O isolado A. flavus CBMAI 1023, foi selecionado para a realização de experimentos de produção a maior escala onde foi possível isolar e caracterizar um novo policetídeo. / From a group of 280 sugarcane-derived fungi 18 strains were assessed for the presence of polyketide synthase genes by PCR approaches. These fungi were identified taxonomically by a polyphasic approach classifying into four orders and nine genres. Biological activity tests showed the presence of antibiotic metabolites against pathogenic microorganisms and the relationship of this activity might be linked with the fungal isolate location by multiple correspondence analyses. 36 sequences similar to PKS genes fragments were detected from 17 of these fungi. A neighbor-joining phylogenetic analysis of the KS domain showed that 16 sequences fit on the monophyletic group of PKS evolved with production of non reduced polyketides, and the other 10 sequences fit on the monophyletic group of PKS evolved with the production of reduced polyketides. CMT domain analysis also pointed that the sequences fit with groups of PKS depending on polyketide reduction grade, all ten related to PKS evolved with the synthesis of reduced polyketides. Protein structural analysis also pointed out that these sequences are closely related with proteins from condensing enzyme family, highlighting the presence of a characteristic helix elbow that bears the cysteine residue responsible for the condensation activity. The fungi were also tested for their capacity of producing lovastatin compounds where chromatographic TLC detected bands from 10 extracts with the same dislocation compared to a lovastatin, but only 6 were confirmed by HPLC. The A. flavus CBMAI (1023) were selected for upscale production experiments, from where it was possible isolate and characterize a new polyketide compound.