The background to the large-scale planting of Pinus contorta in northern Sweden is reviewed with an account of the distribution and characteristics ofPi nus contorta within its natural range in western North America. The threatsto successful planting of exotics are discussed in relation to the historicalbackground. Attention is also drawn to parasitic fungi which are infectingPi nus contorta in western Canada, and to the potential threat they representto the indigenous Pi nus sylvestris in Sweden.During a seven-year-period 100 provenances of Pi nus contorta have been investigatedannually with respect to different kinds of damage, primarilythose by parasitic fungi. The study indicates that damage to Pi nus contortaprimarily occurs during the first ten years after planting. Northern provenancesof Pi nus contorta are generally more resistant to pathogens than southernprovenances. Weather damage occurs almost every year among trees ofsouthern and coastal provenance. Even trees of northern provenance have sufferedfrom weather damage due to temperature oscillations during shoot elongation.Severe weather damage is a predisposing factor to infection by secondarypathogens primarily.Gremmeniella abietina. There is a minor correlation betweensevere weather damage and Phacidium infestans. Even northern provenancesof Pinus contorta are infected by Phacidi um infestans in high altitude standsin northern Sweden. Snow b light infection is, however, of a minor importanceto lodgepole pine than to Scots pine due to the rapid early growth of the former.The most productive plants of both Pinus contorta and Pinus sylvestrisare attacked by Phacidi um infestans. Plants not infected by snow b light havea lower height growth than those infected.Severe infection by Gremmeniella abietina has been recorded after voleattack, even among northern provenances of lodgepole pine. So far Pinus contortahas mainly been infected by the same fungi as Pinus sylvestris, with the |exception of Melampsora pinitorqua and Lophodermella sul ci gena. Pinus contorta iis, however more susceptible to infection by Gremmeniella abietina in connec- !tion with vole damage, depending on the more severe injuries to lodgepole pinethan to Scots pine.So far vole damage has been the most severe threat to Pinus contorta innorthern Sweden. Voles prefer lodgepole pine to Scots pine providing vole populationis moderate. At times of high vole populations even Scots pine suffersdamage. Voles attack Pinus contorta even 14 years after planting. The differencein frequency of vole damage among provenances strongly decreased with increasedvole population and repeated attacks from year to year.Tree tilting was first noted five to eight years after planting on sites exposedto strong winds and severe icing.In the central parts of northern Sweden most provenances of Pinus contortaare less attacked by pathogens than the indigenous Pinus sylvestris, and inorthern provenances of lodgepole pine are remarkably productive in thenorthernmost site, despite a relatively high frequency of Phacidium infestar^.Later investigations indicate, however, more severe damage to Pinuscontorta with increasing latitude and altitude in northern Sweden. / digitalisering@umu

Effects of stand type on ground lichen height and species richness in boreal forests.   P. contorta as an alternative to P. sylvestris in terms of providing a suitable habitat   Maria Johansson     Abstract     Lichen-rich forests are essential to reindeer but up to 50 % of the lichen-rich areas in Sweden have been lost since the 1950s. Ground lichens thrive in pine-heaths and with an increasing area of plantations of the non-native tree species Pinus contorta, as an alternative to the native P. sylvestris, it is important to investigate if P. contorta can provide such an optimal habitat to ground lichens. The purpose of this study was to assess if forest characteristics, such as tree density, canopy cover and production capacity, affect the abundance and growth of five lichen species (Cladonia rangiferina, C. arbuscula/mitis, C. stygia, C. stellaris and Cetraria islandica) and whether these characteristics differ between forests dominated by P. contorta and forests dominated by P. sylvestris. Fieldwork was conducted in Norrbotten and Västerbotten on sample plots previously used by the Swedish National Forest Inventory (SNFI). The statistical analysis was based on data collected from 22 sample plots, 11 of each forest type, visited during July and September 2015. No statistical significant differences were found between forest characteristics of the two forest types, and none of the forest characteristics were found to relate to lichen height. Forest age did not seem to have an effect on ground lichens, while both canopy cover and production capacity were found to negatively relate to the proxy for lichen biomass as well as the abundance of the most common lichen species, C. rangiferina and C. arbuscula/mitis. The result suggests that a shadier canopy as well as a higher production capacity contributes to a reduced distribution of ground lichens.   Keywords: Ground lichens, Pinus contorta, Pinus sylvestris, forest characteristics, forestry, reindeer husbandry

Contorta (Pinus contorta) är ett barrträdslag som naturligt finns i västra Nordamerika och är inplanterat främst i norra Sverige. Syftet med detta arbete är att utvärdera hur långt och i vilken omfattning contorta spridit sig från äldre bestånd i södra Sverige. Resultatet skulle kunna användas vid bedömning om det finns en spridningsrisk i södra Sverige. De tre bestånden som användes vid detta arbete ligger i södra Östergötland och norra Småland. Datainsamlingen utfördes i tre steg i respektive bestånd: 1. Bedömning om contorta kan spridas och etableras från bestånd i södra Sverige. 2. Inventering av contortaplantor i subjektivt valda ytor. 3. Kvantitativ inventering av contortaplantor i systematiskt utlagda linjer, 2 meter breda och 250 meter långa, i fyra riktningar utgående från beståndskant. I inventeringen av subjektivt utvalda ytor hittades contortaplantor 350 meter från contortabestånd och i den kvantitativa inventeringen 50 meter från contortabestånd. Totalt hittades 27 contortaplantor (motsvarande 450 st/ha) i subjektivt utlagda ytor och 18 contortaplantor (45 st/ha) hittades i den kvantitativa inventeringen. Alla funna contortaplantor hittades på störd mark och där trädkronans täckning var noll, dvs. där ljustillgången var högst. Det är däremot inte möjligt att, utifrån denna studie, säga vilken faktor som är primär för contortaplantornas etablering, markstörning eller ljustillgång. / Contorta (Pinus contorta) is a coniferous tree species from western North America that has been introduced mainly in northern Sweden. The purpose of this work was to evaluate how far and to what extent (number of self-dispersal seedlings/ha) Contorta has spread from older populations in southern Sweden. The result can be used in assessing the risk of spreading in southern Sweden. The three forest stands used for this work were located in southern Östergötland and northern Småland, Sweden. The data collection was done in three stages in each stand: 1. Assessment if contorta can be disseminated and established from stocks in southern Sweden. 2. Subjective inventory of Contorta plants in selected areas. 3. Quantitative inventory of contorta plants along defined corridors, 2 meters wide and 250 meters long, in four directions from the edge of the stock. In the inventory of subjectively selected areas, contorta plants were found 350 meters from a contorta stand and in the objective inventory 50 meters from a contorta stand. A total of 27 contorta plants (equivalent to 450 plants/ha) was found in subjectively inventory and 18 contorta plants (45 plants/ha) were found in the quantitative inventory. All Contorta seedlings were found on disturbed ground and where the canopy cover of trees was zero, i.e. where the access to light was highest. On the other hand, based on this study, it is not possible to say which factor is most important for the establishment, field disturbance or light supply.

Pinus Contorta was introduced to Sweden as a tree that would grow fast with a promising future, also a promise in increased production. What could not be anticipated was the common Swedish weather conditions, such as snow and wind, would have negative impact on Pinus contorta due to its instability connected to fast-growing trunk and wide crown. Those predictions were hard to estimate since these weather conditions also is common in Canada, where it has its origins. The overall damage caused by snow and wind generates an annual loss of approximately hundred million Swedish crowns (SEK), which has a great impact on the Swedish forestry economy. The awareness of the risks and damages on Pinus contorta makes it possible to both prevent and avoid these to some extent. A more detailed study has been done in the Swedish region Norrbotten, where the level of the damage on Pinus contorta was compared to the Swedish pine. The study included inventory of un-thinned stands and quantified data was collected. The conclusions indicated that the level of damage on Pinus contorta stands was more than twice the damage of the Pinus sylvestris stands. However, since Pinus contorta is relatively new in Sweden, the results regarding the future of the contorta pine are therefore insufficient. Furthermore, it is not possible to predict if the promised increase of production will ever be achieved. To achieved reliable results, further studies are suggested where both loss of profit, compared to the Pinus sylvestris stands, but also the full turnaround time of Pinus contorta are included.

Lodgepole pine (Pinus contorta Douglas ex Louden) is the most widespread pine in North America and the single most abundant tree species in British Columbia (BC). Its vast distribution, diversity and economic value make it an important species for timber harvest and subsequent reforestation. Climate change raises serious concerns over the adaptability and effective management of BC’s future forests. The majority of lodgepole pine seedlings requested for replanting are produced from seed obtained from wild stands, but the relationship between climate variation and the seed production of natural populations has yet to be assessed. The purpose of this study is to determine if variation in P. contorta seed yield is related to the climate of BC. Historical cone collection data were obtained from archived records of 1948 seedlots in 22 different natural stand seed planning zones (SPZs) of BC. Collections were made between 1963 and 2013 and seed yield (kg fresh seed/hL cone) was determined for each seedlot. First, natural variation in seed production of lodgepole pine was examined in 18 different SPZs. The Nass Skeena Transition (NST) represents a unique intersection between continental and maritime ecosystems and was found to have a significantly higher mean seed yield compared to all other zones, with the exception of Hudson Hope (HH). However, variance in seed yield for NST was found to be an order of magnitude higher than that of other SPZs, indicating that seed production in this region is exceptionally variable. These findings provide a valuable geographic baseline for the reproductive fitness of lodgepole pine, suggesting that climate adaptation and mitigation strategies for some areas of the province may need to be region-specific. In addition, the relationship between climate variation and the seed production of P. contorta in BC was evaluated. The climate of each region was described using dynamically-downscaled Global Circulation Model (GCM) and reanalysis climate output from the Canadian Regional Climate Model (CRCM). Annual, winter, and summer means were explored for each of the climate variables of interest: total precipitation (mm) and surface air temperature (°C). Temporal correlations between the mean annual seed yield anomaly and the anomaly of both climate variables were significant under a variety of climate schemes in a number of SPZs. Significant overall trends in climate variables were also captured using GCM-driven CRCM output. While these two analyses independently highlighted significant relationships between seed yield and climate, their joint implications were unclear. Shifts in the CRCM boundary conditions revealed that the results lacked robustness during the historical period, inhibiting the investigation of future projections. Ambiguous age ranges for each cone collection and temporal restrictions of the seed collection data may be partially responsible for these inconclusive results. Results from the first half of this thesis suggest that, with few exceptions, seed production is relatively stable across SPZs spanning a wide range of climate regimes. Thus, the investigation of the relationship between reproductive fitness and climate may be complicated by the extraordinary adaptability of lodgepole pine and the high genetic variation in natural populations. / Graduate

Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2015-12-02T09:38:29Z No. of bitstreams: 1 texto completo.pdf: 3145359 bytes, checksum: 37020cf2346e9e4fdb1512c81bcfc5e2 (MD5) / Made available in DSpace on 2015-12-02T09:38:29Z (GMT). No. of bitstreams: 1 texto completo.pdf: 3145359 bytes, checksum: 37020cf2346e9e4fdb1512c81bcfc5e2 (MD5) Previous issue date: 2015-07-10 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Cicatrização de ferida é um processo sistêmico e dinâmico que envolve as etapas de inflamação, proliferação e remodelação e, tem por objetivo restaurar a continuidade do tecido. Embora a reparação tecidual seja um processo sistêmico, é necessário favorecer condições locais por meio de terapia tópica adequada para viabilizar o processo fisiológico. Na medicina popular, tinturas das cascas das espécies arbóreas de Inga subnuda (ingá) e Pseudopiptadenia contorta (angico) são utilizadas no tratamento de feridas cutâneas. Tendo em vista esse uso popular, este trabalho tem como objetivo avaliar o efeito destas plantas na cicatrização de feridas cirúrgicas induzidas em coelhos. Foram preparados extratos hidroalcoólicos das cascas de ingá e angico por maceração, e realizadas análises de prospecção fitoquímica e atividade antioxidante pelo método de DPPH. Preparados, os extratos de ingá e angico foram adicionados a Creme Lanette 24% até atingir as concentrações de 1% e 5%. Foram utilizados 36 coelhos machos, da raça Albino Nova Zelândia, 60 dias de vida e peso médio de 2,8 Kg (CEUA/UFV, no064/2012). Duas feridas cirúrgicas de dimensão 1,5 x 1,5 cm foram produzidas na região dorsal-torácica dos animais e tratadas durante 14 dias. A primeira foi confeccionada com lâmina de bisturi e denominada Ferida e a segunda com bisturi elétrico, denominada Queimadura. Os animais, divididos em grupos de seis animais, foram tratados com solução salina; extrato de ingá 1%; extrato de ingá 5%, extrato de angico 1%; extrato de angico 5% e pomada Fitoscar®. Índice de contração da ferida (IC) foi medido no 7o, 10 e 14o dia pós-operatório. Após o 14o dia de tratamento os animais foram eutanasiados e amostras de sangue coletadas para análise bioquímica e hematológica. Em seguida, amostras das feridas e queimaduras foram extraídas cirurgicamente, processadas rotineiramente e coradas com hematoxilina-eosina e picrosirrius. O parâmetro estereológico de densidade volumétrica foi calculado para contagem de fibroblasto, vaso sanguíneo, células inflamatórias, e índice de maturação do colágeno (IMaC). Os resultados foram analisados pela ANOVA, seguido pelo teste de Tukey. Resultados da prospecção fitoquímica e de DPPH mostram presença de flavonoides, taninos e saponinas, e, atividade antioxidante dos extratos de ingá e angico. Os resultados hematológicos e bioquímicos mostram que os animais tratados com os extratos vegetais não apresentaram infecção sistêmica e nem alterações hepáticas e renais. Os parâmetros estereológicos indicam que os extratos de ingá e angico contribuíram para a promoção da fibroplasia, angiogênese e maturação do colágeno nas feridas cirúrgicas. Pelo índice de contração, conclui-se que os extratos de ingá e angico apresentaram efeito cicatrizante tanto na ferida quanto na queimadura, sendo mais efetivos na fase proliferativa. Este estudo forneceu evidências do efeito cicatrizante dos extratos de ingá e angico e do potencial uso como medicamento fitoterápico. Porém, mais estudos são necessários para elucidar o mecanismo de ação destes extratos no processo terapêutico. / Wound-healing is a systemic and dynamic process involving the steps of inflammation, proliferation and remodeling, and aims to restore the continuity of the tissue. Although the tissue repair is a systemic process, it is necessary to offer local conditions by appropriate topical therapy to enable the physiological process. Traditionally, bark extract of Inga subnuda (inga) and Pseudopiptadenia contorta (angico) has been used on the treatment of skin wounds. The objective of this study was to investigate the healing effect of the extract inga and angico on the surgicals wounds induced in rabbits. Barks extract of inga and angico was extracted by maceration, and they were carried out analysis of phytochemical prospecting and antioxidant activity by DPPH method. Thirty-six male rabbits, type Albino New Zealand, 60 days, weighing 2,8Kg, from the Federal University of Viçosa were used (CEUA/UFVn°064/2012). Two surgical wounds of 1.5 x 1.5 cm were made in the dorsal thoracic region. The first wound was made by removing the skin with a scalpel blade, called Wound, and the second wound with an electric scalped, called Burn. Animals were treated for 14 days. The animals were randomized in groups of six rabbits and were treated with saline solution; extract of inga 1%; extract of inga 5%, extract of angico 1%; extract of angico 5% and Fitoscar® ointment. Wound contraction index (WCI) was measured on the 7th, 10th and 14th post-wounding. After 14 days of treatment the animals were euthanized and blood samples collected for biochemical and hematological analysis. Samples of wounds and burns were taken, processed routinely, and stained with hematoxylin-eosin and picrosirrius. The stereological parameters of bulk density was calculated by counting of fibroblasts, blood vessels and inflammatory cells and collagen maturation index.The results were analyzed by ANOVA and by Tukey test. The results of phytochemical prospecting and DPPH show the presence of flavonoids, tannins, saponins and antioxidant activity. The hematological and biochemical results show that animals treated with plant extracts showed no systemic infection or hepatic and renal disorders.The stereological parameters indicate that inga and angico extracts contributed to promoting fibroplasia, angiogenesis and maturation of collagen in surgical wounds. by contraction index, it is concluded that inga and angico extracts has healing activity on the wound and burn, significantly accelerating healing in the proliferative phase. This study provided evidence of the healing effect of the extracts inga and angico and potential use as herbal medicine, but more studies are needed to elucidate the mechanism of action of these extracts in the therapeutic process.

Native plant communities and agricultural land are commonly converted to urban areas as cities across the Western United States continue to grow and expand. This expansion is typically accompanied by afforestation where a common goal among communities is to maximize shade tree composition. Planted forests in these regions are commonly composed of introduced tree species native to mesic environments and their ability to persist is dependent on consistent irrigation inputs. Many potential ecosystem services may be derived from planting trees in urban and suburban areas; however, there are also costs associated with extensive afforestation, and shade tree cover may have significant implications on municipal water budgets. In this study I evaluate variation in daily and seasonal water use of regionally common suburban landscape tree species in the Heber Valley (Wasatch County, Utah). I had two primary objectives: (1) to identify and understand the differences in transpiration between landscape tree species in a suburban setting and (2) to assess the sensitivity of sap flux and transpiration to variation in vapor pressure deficit, wind speed, and incoming shortwave radiation. I used Granier's thermal dissipation method to measure the temperature difference (ΔT) between two sap flux probes. The empirical equation developed by Granier was used to convert ΔT into sap flux density (Jo) measurements, which were then scaled to whole-tree transpiration. There were consistent and substantial differences in sap flux between tree species. I found that Picea pungens under irrigated growing conditions, on average, had Jo rates that were 32% greater and whole tree water use (ET) rates that were 550% greater than all other species studied. The findings of Jo may be partially explained by xylem architecture and physiological control over stomatal aperture. However, the rate of water flux in the outermost portion of sapwood does not necessarily determine the magnitude of whole tree transpiration. Rather, ET in this study was largely explained by the combined effects of irrigation, tree size, and sapwood to heartwood ratio.

Nutrient dynamics in a lodgepole pine forest at Bootleg Mountain, B.C., were investigated through the sampling of soil, snow and groundwater in six one-ha blocks. Nitrogen (NO3-, NH4+, TIN, TDN, TN), phosphorus (PO43-, TDP, TP), and DOC were analyzed in addition to N mineralization and nitrification. Position and dispersion statistics were computed for each variable and correlations (Pearson and Spearman) were computed for each pair of variables. The overall heterogeneities of soil, snow, and groundwater were generally lower between 1-ha blocks than between plots. Productivity in the soil was generally N-limited with low input from snow precipitation. Very little N leached from soil to groundwater. Phosphorus contents were highly variable and were the limiting nutrient in the groundwater. Rates of net and gross N mineralization and nitrification were determined using buried bags and 15N isotope dilutions. Gross rates were greater than net rates and nitrification was low relative to high immobilization rates. The N cycle appears to be tightly regulated, thus further study will be needed to monitor the impact of harvesting on N cycling.

Nutrient dynamics in a lodgepole pine forest at Bootleg Mountain, B.C., were investigated through the sampling of soil, snow and groundwater in six one-ha blocks. Nitrogen (NO3-, NH4+, TIN, TDN, TN), phosphorus (PO43-, TDP, TP), and DOC were analyzed in addition to N mineralization and nitrification. Position and dispersion statistics were computed for each variable and correlations (Pearson and Spearman) were computed for each pair of variables. The overall heterogeneities of soil, snow, and groundwater were generally lower between 1-ha blocks than between plots. Productivity in the soil was generally N-limited with low input from snow precipitation. Very little N leached from soil to groundwater. Phosphorus contents were highly variable and were the limiting nutrient in the groundwater. Rates of net and gross N mineralization and nitrification were determined using buried bags and 15N isotope dilutions. Gross rates were greater than net rates and nitrification was low relative to high immobilization rates. The N cycle appears to be tightly regulated, thus further study will be needed to monitor the impact of harvesting on N cycling.

I dagsläget finns ca 600 000 ha Contortaskog i Sverige varav en stor del planterades runt 70- till 80-talet. Contorta har sitt ursprung i västra USA och Kanada och introducerades i Sverige som en lösning på en förutspådd fibersvacka. I dagsläget börjar en stor del av den inplanterade Contortan börjar bli så pass gammal att det går att ta fram sågade trävaror ur stocken men exakt vilka egenskaper svenskvuxen Contorta har inte helt redogjort. I denna studie har korrelationen mellan medelårsringsbredden, sommarvedsandelen, svällning och böjhållfastheten, e-modulen och densiteten undersökts. Dessutom undersöktes det om det finns en statistisk skillnad mellan de fyra växtplatserna hos Contortan i studien och hur Contorta skiljer sig mot svensk tall och gran. Korrelationen mellan medelårsringsbredden och böjhållfastheten var -0,33, densiteten -0,18 och E-modulen -0,42. För sommarvedsandelen var korrelationen till böjhållfastheten 0,11, densiteten 0,21 och E-modulen 0,06. För radiell respektive tangentiell svällning var korrelationen till böjhållfastheten -0,12 och -0,15, densiteten 0,01 och 0,11, E-modulen -0,29 och 0,27. För alla fyra växtplatser fanns det en statiskt signifikant skillnad mellan medelvärdena för alla undersökta variabler. Svensk tall och Gran har liknande samband. / Currently there is about 600 000 hectares of lodgepole pine forests in Sweden which of most were planted during the 1970s and -80s. Lodgepole pine is native species to western USA and Canada and was introduced in Sweden as an answer to an expected pulp-fiber volume depression. A big portion of these stands are now reaching maturing age for sawn timber product production, but little is known about lodgepole pine grown on Swedish soil. In this study, the correlation between the mean tree ring width, latewood ratio, swelling and the bending strength, modulus of elasticity (MOE) and density have been studied, if there is a statistically significant difference between the four different geographical stands the lodgepole pine in this study are stemming from and lastly how Swedish grown lodgepole pine differs from the traditional Swedish construction lumbers Scots pine and Norway spruce. The correlation between the mean ring width and the bending strength was -0,33, the density -0,18 and the MOE -0,42. For the latewood ratio and the bending strength it was 0,11, the density 0,21 and the MOE 0,06. For the radial and tangential swelling respectively it was -0,12 and -0,15 for the bending strength, 0,01 and 0,11 for the density and -0,29 and 0,27 for the MOE. For all four different growth stands there was a statistically significant difference between the means for all studied variables. The relationships for both Scots pine and Norway spruce show similar magnitudes and directions.