A prevenção das doenças periimplantares baseia-se em procedimentos de rotina na clínica, com a remoção mecânica do biofilme associada ou não de agentes antimicrobianos locais e sistêmicos. No entanto, o controle do biofilme oral sobre a superfície de implantes é dificultado particularmente sobre as superfícies rugosas do titânio, que apesar de facilitarem a osseointegração, também favorecem a formação do biofilme. Objetivos: Objetivou-se analisar in vitro o efeito da TFD (Terapia fotodinâmica) usando curcumina como fotossensibilizador sobre o biofilme formado na superfície de discos de titânio com superfície rugosa e lisa. Material e Métodos: Biofilme mono espécie de Streptococus gordonii, colonizador inicial do biofilme dental, foi formado em superfícies lisas (usinadas) e rugosas (tratadas) de discos de titânio por 72 horas. Os discos lisos e rugosos foram então distribuídos em 3 grupos: tratados por 5 minutos com curcumina (60?M), clorexidina 0,12% como controle positivo e solução de DMSO (dimetilsufóxido), etanol e água como controle negativo (veículo da curcumina). As amostras foram divididas em 2 sub-grupos: ativado por LED azul (455-492nm) por 7 minutos e não-ativado. A atividade metabólica do biofilme foi determinada pelo método de redução do Sal tetralisol XTT e os resultados analisados estatisticamente pela análise da variância (ANOVA) seguida pelo teste de Tukey (p < 0,05). Resultados: Na ausência de luz, apenas o tratamento com clorexidina promoveu redução microbiana (controle positivo). O tratamento com curcumina ativada pela luz azul reduziu o número de bactérias viáveis no biofilme em 71% nos discos lisos e em 77% nos rugosos, não havendo diferença estatística com o controlepositivo.Conclusão: Os dados in vitro indicam que TFD associada à curcumina tem atividade sobre biofilme microbiano, sugerindo seu potencial no controle do biofilme oral sobre a superfície de titânio, e que este efeito independe da topografia da superfície. / Prevention of periimplant diseases is based on routine procedures in the clinic, with the mechanical removal of biofilm associated or not with local and systemic antimicrobial agents. However, the control of oral biofilm on the surface of implants is particularly difficult on the rough surfaces of titanium, which, although facilitating osseointegration, also favors biofilm formation. Objectives: The objective of this study was to analyze in vitro the effect of PDT using curcumin as a photosensitizer on the biofilm formed on the surface of titanium discs with a rough and smooth surface. Material and Methods: Biofilm mono species of Streptococus gordonii, initial colonizer of the dental biofilm, was formed on smooth (machined) and rough (treated) surfaces of titanium discs for 72 hours. The smooth and rough discs were then divided into 3 groups: treated for 5 minutes with curcumin (60 ?M), 0.1% chlorhexidine as a positive control and DMSO, ethanol and water solution as a negative control. The samples were divided into 2 subgroups: activated by blue LED (455-492nm) for 7 minutes and non-activated. The metabolic activity of the biofilm was determined by the reduction XTT salt method and the results analyzed statistically by ANOVA followed by the Tukey\'s test (p <0.05) Results: In the absence of light, only the chlorhexidine treatment promoted microbial reduction (positive control). The blue light-activated curcumin treatment reduced the number of viable bacteria in the biofilm in 71% in the smooth discs and in 77% in the wrinkles, and there was no statistical difference with the positive control. Conclusion: In vitro data indicate that PDT associated with curcumin has activity on microbial biofilm, suggesting its potential in the control of oral biofilm on the titanium surface, and that this effect is independent of surface topography.

Les crises énergétiques, en particulier celles liées aux combustibles fossiles, conduisent à une utilisation accrue des nouvelles énergies renouvelables. De plus, les recherches visent également à utiliser des matériaux de stockage de l'énergie qui sont plus efficace, tels que les batteries, et à utiliser des matériaux économes en énergie. Une OLED (diode électroluminecente organique)) est une diode émettrice la lumière (LED) qui sont connues grâce leur potentiel élevé dans les applications d'affichage, de signalisation et d'éclairage. Parmi ces matériaux organiques, les caroténoïdes constituent une classe importante de molécules linéaires conjuguées qui présentent un degré de délocalisation électronique élevé et de dynamique ultra-rapide. Le colorant joue un rôle important dans le fonctionnement des cellules solaires sensibilisées par colorant (CSSCs) ou d'autres matériaux bi fonctionnels. Dans cette recherche, nous avons étudié la photo stabilité des caroténoïdes (-carotène et fucoxanthine) à l'aide du kaolin modifié et de l'antioxydant. La photostabilité du -carotène et de la fucoxanthine peut être améliorée par la kaolinite modifiée et l'antioxydant. La fabrication OLED utilisant la fucoxanthine n'est pas suffisante à cause de la bande interdite de la fucoxanthine (Fx) qui est très grande et peut être un isolant. NPD (50 nm) / Fx (1 nm) / Alq3 (85 nm) a un EQE de 0,12% et un CIE (0,416 ; 0,5302). Les dispositifs OLEDs en utilisant le ß-carotene comme couche de transport du trou et la curcumine comme couche émettrice ont donné une couleur jaune avec un EQE de 0,02%. / Energy crises, especially fossil fuel-based energy, lead to increased use of new energy and renewable energy. In addition, research is also directed towards more efficient energy use through the development of energy storage materials such as batteries, and the use of energy-efficient materials. An OLED (organic light-emitting diode) is a light-emitting diode (LED) and is known for their high potential in a display, signage, and lighting applications. Carotenoids constitute an important class of linear -conjugated molecules that exhibit a high degree of electronic delocalization and ultrafast dynamic. The dye that is used as a photosensitizer plays an important role in the operation of DSSCs or other bifunctional material. In this research, we studied the photostability of the carotenoid compound (-carotene and fucoxanthin) using natural antioxidant and modified kaolinite. In this work, the carotenoid stability by the carotenoid compound/antioxidant binary is studied for the first time. Photostability of -carotene can be enhanced by antioxidant and modified kaolinite. Photoprotection efficiency of -carotene by curcumin was higher than fucoxanthin. Modified kaolinite decreased photodegradation of - carotene by shielded and protected from direct UV irradiation. Antioxidant fucoxanthin and curcumin decreased the electrochemical gap of the binary material. The electrochemical gap of carotene/curcumin is -1.61 eV, carotene/fucoxanthin is -1.75 eV compare to the only -carotene - 2.04 eV. Fucoxanthin can keep the first oxidation stage of -carotene. Therefore at the binary compound, electron was still reversible, but not for curcumin. Fucoxanthin in OLED devices NPD/Fx/ETL reduced significanly the EQE (%) almost 80% in Alq3 and 76% in BAlq. OLED device: NPD(50nm)/Fx(1nm)/Alq3(85nm) yielded an effeciency quantum external yield, EQE = 0.12% and CIE (0.4160, 0.5302). Fabrication OLED using -carotene as HTL and curcumin as EL layer resulted yellow color , EQE = 0.02%, with the composition MoO3(15nm)/NPD(40nm)/Car(10nm)/ Cur(15nm)/Alq3(70nm)/Ca(100nm). For application of - carotene in OLED device, its perfomance can be improved by using curcumin at the fabrication.

Submaximal exercise performance is, in part, limited by the accumulation of metabolic byproducts and energy system capacities. Curcumin and the combination of curcumin and fenugreek soluble fiber (CurQfen®) have been shown to increase endogenous antioxidants and metabolic byproduct clearance as well as reduce inflammation and lipid peroxidation, and therefore, may enhance submaximal aerobic thresholds. In addition, there is evidence that the galactomannan component of fenugreek, used to enhance bioavailability of curcumin, may also have potential physiological effects related to the up regulation of free fatty acid oxidation Therefore, the purpose of this study was to examine the effects of curcumin and fenugreek soluble fiber supplementation on the ventilatory threshold (VT), respiratory compensation point (RCP), maximal oxygen consumption (O2 peak), and time to exhaustion (Tlim)derived from a graded exercise test (GXT). Forty-five untrained, college-aged, male (n = 24) and female (n = 21) subjects (mean age ± SD: 21.2 ± 2.5 yr) were randomly assigned to one of three supplementation groups; placebo (PLA, n=13), 500 mg·day-1 CurQfen® (CUR, n=14), or 300 mg·day-1 fenugreek soluble fiber (FEN, n=18). All of the subjects completed a maximal GXT on a cycle ergometer to determine the VT, RCP, O2 peak, and Tlim before (PRE) and after (POST) 28 days of daily supplementation. The VT and RCP were determined from the V-slope method for the ventilation (E)vs. O2 and E vs. CO2, respectively. Separate, one-way ANCOVAs were used to examine the between group differences for adjusted POST VT, RCP, O2 peak, and Tlim values, with the respective PRE test value as the covariate. The adjusted POST VT-O2 for the CUR (mean ± SD= 1.593 ± 0.157 L·min-1) and FEN (1.597 ± 0.157L·min-1) groups were greater than (p= 0.04 and p= 0.03, respectively) the PLA (1.465 ± 0.155L·min-1) group, but the FEN and CUR groups were not different (p = 0.94). The one-way ANCOVAs for RCP (F = 3.177, p = 0.052), O2 peak (F = 0.613, p = 0.547), and Tlim (F = 0.654, p = 0.525) indicated there were no significant differences among groups. These findings suggested that CurQfen® and/or fenugreek soluble fiber may improve submaximal, but not maximal, aerobic performance indices in untrained subjects.

The food that we consume contain many dietary compounds which are biologically active. In this thesis we will discuss the biological effects of dietary compounds and the mechanisms behind their activities. First, we studied on the anti-metastatic effects of curcumin, a dietary compound derived from turmeric, through lymphangiogenesis inhibition. Curcumin inhibited vascular endothelial growth factor-C (VEGF-C)-induced lymphangiogenesis in vivo and in vitro. Curcumin inhibited lymphangiogenesis, in part through suppression of proliferation, cell cycle progression and migration of lymphatic endothelial cells. Curcumin inhibited expressions of VEGF receptors (VEGFR2 and VEGFR3), as well as down-stream signaling such as phosphorylation of ERK and FAK. Finally, curcumin sulfate and curcumin glucuronide, two major metabolites of curcumin in vivo, had little inhibitory effect on proliferation of HMVEC-dLy cells. Our results demonstrate that curcumin inhibits lymphangiogenesis in vitro and in vivo, which could contribute to the anti-metastatic effects of curcumin. Next, we investigated the mechanisms underlying the cytotoxic activity of tert-butylhydroquinone (TBHQ), a widely used synthetic food antioxidant. Here we found that the biological effects of TBHQ are mainly mediated by its oxidative conversion to a quinone metabolite tert-butylquinone (TBQ). Co-addition of cupric ion (Cu2+) enhanced, whereas ethylenediaminetetraacetic acid (EDTA) suppressed the oxidative conversion of TBHQ to TBQ, and the biological activities of TBHQ in MC38 colon cancer cells. Finally, a structure and activity relationship study was done and together, these results suggest that the biological activities of TBHQ and other para-hydroquinones are mainly mediated by their oxidative metabolism to generate more biologically active quinone metabolites.

No description available.

Orientador: Iguatemy Lourenço Brunetti / Resumo: O diabetes mellitus (DM) é uma síndrome metabólica crônica, caracterizada principalmente por hiperglicemia. A hiperglicemia crônica é um dos principais fatores envolvidos na instalação do estresse glico-oxidativo, o qual está implicado no desenvolvimento das complicações micro e macrovasculares do DM, as quais resultam em morbidade e mortalidade significativas. Portanto, o tratamento da doença tem como principal objetivo a manutenção dos níveis glicêmicos próximos a normalidade e a atenuação do estresse glico-oxidativo. A curcumina, apresenta potencial efeito antidiabético e antioxidante. Já a aminoguanidina é um dos agentes mais utilizados para prevenir a formação de produtos finais da glicação avançada (AGE), in vitro e em modelos animais. Esse estudo investigou em ratos, o potencial antidiabético da curcumina e/ou aminoguanidina incorporadas no iogurte, avaliando parâmetros relacionados às alterações do controle glicêmico, estresse glico-oxidativo (plasma, fígado e rim) e parâmetros da função neutrofílica (exsudato peritoneal). Ratos Wistar, não diabéticos e diabéticos (estreptozotocínico) não tratados, receberam apenas iogurte e os diabéticos tratados receberam: insulina (4U/dia); 90 mg/kg de curcumina; 50 mg/kg de aminoguanidina; 90 mg/kg de curcumina e 50 mg/kg de aminoguanidina; 100 mg/kg de aminoguanidina; e 90 mg/kg de curcumina e 100 mg/kg de aminoguanidina; os tratamentos foram realizados durante 45 dias. Os parâmetros analisados foram: (i) fisiológicos: peso corpo... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Diabetes mellitus (DM) is a chronic metabolic syndrome characterized mainly by hyperglycemia. Chronic hyperglycemia is one of the main factors involved in the installation of glycoxidative stress, and it is implicated in the development of micro and macrovascular complications of DM, which result in significant morbidity and mortality. Therefore, the treatment of the disease has as main objective the maintenance of glycemic to levels near to normality and the attenuation of glycoxidative stress. Curcumin has potential antidiabetic and antioxidant effects. Aminoguanidine is one of the most used agents to prevent the formation of advanced glycation end products (AGE), in vitro and animal models. This study investigated in rats the antidiabetic potential of curcumin and/or aminoguanidine incorporated into yoghurt, evaluating parameters related to changes in glycemic control, glycoxidative stress (plasma, liver and Kidney) and parameters of neutrophil function (peritoneal exudate). Rats Wistar, non-diabetic and diabetic (streptozotocinic) untreated received alone yoghurt and treated-diabetic received: insulin (4U/day); 90 mg/kg curcumin; 50 mg/kg aminoguanidine; 90 mg/kg curcumin and 50 mg/kg aminoguanidine; 100 mg/kg aminoguanidine; and (vi) 90 mg/kg curcumin and 100 mg/kg aminoguanidine; the treatments were performed for 45 days. The parameters analyzed were: (i) physiological: body weight; (ii) biochemicals: glycemia, glycated hemoglobin (HbA1c), fructosamine, total cholestero... (Complete abstract click electronic access below) / Doutor

Orientador: Morgana Rodrigues Guimarães Stabili / Resumo: Curcumin é um composto ativo derivado da planta Curcuma longa que exibe uma variedade de atividades biológicas, potencial anti-inflamatório e imunomodulatório, que pode ser associado ao tratamento de uma série de condições, como doenças inflamatórias intestinais, artrite reumatoide, câncer, diabetes e periodontite. Recentemente, a literatura também tem demonstrado o potencial do composto sobre o reparo ósseo, entretanto, os dados ainda são contraditórios. O objetivo deste estudo é avaliar o efeito da administração tópica do curcumin veiculado em nanopartículas de PLGA sobre a formação/reparo ósseo de defeitos críticos in vivo, e investigar seu potencial biológico in vivo e in vitro. Defeitos ósseos confeccionados na calvária de ratos machos foram preenchidos com solução salina (controle), curcumin nanoparticulado 0,05mg/ml ou veículo utilizado na diluição do composto (nanopartícula vazia) por 7, 14 e 28 dias. A fração de volume ósseo no interior do defeito nos diferentes grupos foram avaliados por microtomografia óssea (μCT); expressão e imunolocalização dos marcadores do turnover tecidual (RANKL, OPG, PCNA e RUNX2) foram investigados por imuno-histoquímica, enquanto as características teciduais do tecido neoformado foram observadas através da análise histológica descritiva e estereometria. In vitro, o potencial do curcumin nanoparticulado em estimular a diferenciação osteogênica de células estromais derivadas da medula óssea (BMSCs) de ratos foi avaliado determinando-se a at... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Curcumin is an active compound derived from the Curcuma longa plant that exhibits a variety of biological activities, anti-inflammatory and immunomodulatory potential, which can be associated with the treatment of a number of conditions, such as inflammatory bowel diseases, rheumatoid arthritis, cancer, diabetes and periodontitis. Recently, the literature has also demonstrated the potential of the compound on bone repair, however, the data are still contradictory. The aim of this study is to evaluate the effect of topical administration of curcumin-loaded PLGA nanoparticles on bone formation / repair of critical defects in vivo, and to investigate its biological potential in vivo and in vitro. Bone defects made in the calvaria of rats were filled with saline solution (control), nanoparticulate curcumin 0,05mg/ml, or vehicle used in the dilution of the compound (empty nanoparticle) for 7, 14 and 28 days. The fraction of bone volume within the defect in the different groups was assessed by microcomputed tomography (μCT); expression and immunolocation of tissue turnover markers (RANKL, OPG, PCNA and RUNX2) were investigated by immunohistochemistry, while the tissue characteristics of the newly formed tissue were observed through descriptive histological analysis and stereometry. In vitro, the potential of nanoparticulate curcumin in stimulating osteogenic differentiation of rat bone marrow-derived stromal cells (BMSCs) was assessed by determining the activity of alkaline phospha... (Complete abstract click electronic access below) / Mestre

Orientador: Amanda Martins Baviera / Resumo: Introdução: Ativos naturais têm sido estudados por suas ações antioxidantes, anti-inflamamatórias e antiglicação, que podem ser interessantes para prevenir/atenuar as complicações da obesidade e do diabetes mellitus (DM). A curcumina (polifenol isolado de Curcuma longa L.) e a trigonelina (alcaloide isolado de Trigonella foenum-graecum L.) apresentam ações benéficas contra os sintomas e desordens da obesidade e do DM, e combatem as consequências deletérias relacionadas ao estresse glicoxidativo. Objetivo: Investigar, em camundongos submetidos ao modelo experimental de obesidade/resistência à insulina, os efeitos dos tratamentos com trigonelina e/ou curcumina em iogurte, avaliando alterações em parâmetros fisiológicos e bioquímicos, sinalização insulínica (AKT), programa termogênico e status energético [sirtuína 1 (SIRT 1), proteína quinase ativada por adenosina monofosfato (AMPK), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), proteína desacopladora mitocondrial 1(UCP 1)], níveis de marcadores de estresse glicoxidativo [substâncias reativas ao ácido tiobarbitúrico (TBARS), produtos finais de glicação avançada (AGEs), receptor de AGEs (RAGE)], marcadores da inflamação [fator nuclear kappa B (NF-кB), interleucinas (IL)-6 (IL-6) e 10 (IL-10), fator de necrose tumoral (TNF-α)], potencial antioxidante [atividades de superóxido dismutase (SOD), catalase (CAT), glutationa peroxidase (GSH-Px), nuclear factor (erythroid-derived 2)-like-2 factor (NRF 2)]... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Introduction: Natural actives have been studied for their antioxidant, anti-inflammatory, and antiglycation potentials, which may be interesting to prevent/attenuate the complications of obesity and diabetes mellitus (DM). Curcumin (polyphenol isolated from Curcuma longa L.) and trigonelline (alkaloid isolated from Trigonella foenum-graecum L.) have beneficial actions against the symptoms and disorders of obesity and DM, and act against the deleterious consequences related to glycoxidative stress. Objectives: To investigate the effects of treatments of mice submitted to the experimental model of obesity/insulin resistance with trigonelin and/or curcumin in yoghurt, evaluating the changes in physiological and biochemical parameters, insulin signaling (AKT), thermogenic program and energetic status [sirtuin 1 (SIRT 1), adenosine monophosphate-activated protein kinase (AMPK), peroxisome proliferator-activated gamma coactivator 1-alpha receptor (PGC-1α), mitochondrial uncoupling protein 1 (UCP 1), advanced glycation end products (AGEs), receptor for AGEs (RAGE)], markers of inflammation [nuclear factor kappa B (NF-кB), interleukins (IL) IL-6 and IL-10, tumor necrosis factor (TNF-α)], antioxidant defenses [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), nuclear factor (erythroid-derived 2)-like-2 factor (NRF2)], AGEs detoxification machinery [glyoxalase 1 (GLO 1), AGE receptor 1 (AGE-R1)]. Methods: Male C57BL/6 mice were fed a standard diet (P, 3.85 kc... (Complete abstract click electronic access below) / Doutor

Orientador: Luis Octavio Regasini / Resumo: As micoses superficiais e cutâneas são infecções fúngicas que acometem a camada mais externa do estrato córneo da pele e seus anexos. São as micoses mais frequentes dentre todas as infecções fúngicas e estão distribuídas por todo o mundo, afetando todas as faixas etárias. O impacto primário destas micoses é seu aspecto visual com estética negativa. Contudo, não se restringem apenas ao aspecto estético, vindo a ser um grave problema de saúde na população neonatal, senescente e de imunocomprometidos. Além disso, muitos fármacos exibem toxicidade alta, interações medicamentosas e alto custo econômico. As recidivas por meio do tratamento convencional costumam ser frequentes, as quais podem ser ocasionadas por cepas resistentes. Esse cenário torna premente a busca por antifúngicos inovadores. No presente estudo avaliou-se atividade antifúngica de 19 curcuminoides, provenientes da composteca do Laboratório de Antibióticos e Quimioterápicos (LAQ), contra fungos filamentosos dermatofíticos dos gêneros Trichophyton, Epidermophyton e Microsporum e leveduriformes (Candida spp.), de cepas de referência ATCC e de isolados clínicos, provenientes da coleção do Laboratório de Microbiologia da Faculdade de Medicina de São José do Rio Preto (FAMERP), bem como avaliou-se toxicidade dos curcuminoides mais potentes contra células de queratinócitos humanos (HaCat) e larva G. mellonella. Afim de verificar a atividade antifúngica dos compostos, foram determinados os valores: Concentração Inibitória ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Superficial and cutaneous mycoses are fungal infections that affect the outermost layer of the stratum corneum of the skin and its attachments. They are the most frequent mycoses among all fungal infections and are distributed all over the world. The first impact of these mycoses is their visual appearance with a negative aesthetic. However, they are not restricted to the aesthetic aspect, becoming a serious health problem in the neonatal, senescent and immunocompromised population. In addition, many drugs exhibit high toxicity, drug interactions and high economic cost. Relapses through conventional treatment are often frequent, which can be caused by resistant strains. This scenario demands the development of potent but safer compounds. Herein, we described the antifungal activity of a novel curcuminoid against dermatophyte and Candida species. A series of nineteen curcuminoid analogs was submitted to antifungal assay to assess MIC (minimum inhibitory concentration) and MFC (minimum fungicidal concentration) determined by the broth microdilution method. First, all compound were tested at 31.2 µg mL-1 in order to select the most active compound. The compound 3, 3'-Dimethoxycurcumin (DMC) showed the highest growth-inhibitory activity the largest spectrum of action against dermatophyte clinical isolates and reference strains of Candida tropicalis ATCC and Trichophyton rubrum CBS, with MIC values range of 1.9±31.2 µg mL-1. DMC exhibited fungistatic activity. Inverted microscope ... (Complete abstract click electronic access below) / Mestre

Orientador: Luis Octávio Regasini / Resumo: O câncer é caracterizado por uma proliferação celular descontrolada, desencadeada por diversas mutações genéticas, fatores epigenéticos e ambientais. Apesar dos avanços terapêuticos, esta doença continua a ser responsável por índices de mortalidade elevados em todo o mundo, que é agravado pela grande resistência aos fármacos. Desta forma, se faz necessária a busca por novas substâncias potentes, seletivas e capazes de agir sobre células tumorais resistentes. Os produtos naturais têm despertado interesse científico devido suas inúmeras bioatividades. Dentre estes, a curcumina, isolada dos rizomas de Curcuma longa (popularmente conhecida como açafrão), o cinamaldeído, responsável pelo odor e sabor da canela (Cinnamomum sp.) e as chalconas, flavonoides abundantes em frutas, legumes e plantas, possuem potentes atividades antitumorais contra diversos tipos de câncer. O objetivo deste trabalho foi: a) avaliar a capacidade de inibição da proliferação celular de doze híbridos moleculares de curcumina-cinamaldeído contra células de câncer de mama MCF-7 e MDAMB-231, câncer de colo do útero C33, HeLa, SiHa e CaSki e câncer de vulva A431; b) selecionar o híbrido mais ativo e investigar os possíveis mecanismos celulares e moleculares em linhagens de osteossarcoma U2OS (p53 selvagem) e SAOS (p53 ausente). Pelo ensaio de MTT foi observado que os híbridos foram mais potentes que o cinamaldeído, sendo que híbridos hidroxilados foram mais ativos do que os híbridos metoxilados. O híbrido 5a apr... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Cancer is characterized by uncontrolled cell proliferation, triggered by several genetic mutations. Despite the therapeutic advances, this disease continues to be responsible for high mortality rates worldwide. This fact is aggravated by high drug resistance and combination therapy. Thus, it is necessary to search for new substances potent, selective and able to act on tumor cells resistant. Natural products have aroused scientific interest due to their innumerable bioavailabilities. Among these, curcumin, isolated from Curcuma longa rhizomes (popularly known as saffron) and cinnamaldehyde, responsible for the cinnamon odor and flavor (Cinnamomum sp.) and chalcones, flavonoids abundant in fruits, vegetables and plants, have potent anti-tumor activities against various cancers. The objective of this study was: a) evaluate the ability of inhibit cell proliferation of twelve curcumincinnamaldehyde molecular hybrids against MCF-7 and MDA-MB-231 breast cancer cells, C33, HeLa, SiHa and CaSki cervical cancer and A431 vulvar cancer; b) select the most active hybrid and investigate possible cellular and molecular mechanisms in osteosarcoma cell lines U2OS (p53 wild) and SAOS (p53 null). By the MTT assay it was observed that the hybrids were more potent than the cinnamaldehyde, with hydroxyl hybrids being more active than the methoxylated hybrids. Hybrid 5a presented higher antiproliferative potency against the cells evaluated for women cancer except for MCF-7, with IC50 values rangin... (Complete abstract click electronic access below) / Doutor