An assay for catheptic activity of lamb gastric tissue extract has been proposed which involves the use of a pH 3.5 hemoglobin substrate following activation of zymogens at pH 2.0, 25C for 30 min.; and inactivation at pH 8.0, 40C for 30 min thereby eliminating the effects of pepsin and rennin. Cathepsin was isolated and purified by ammonium sulfate fractionation, acetone precipitation and gel viii filtration. The purified cathepsin represented approximately 50 fold increase in specific activity over the original extract and a recovery of 15% of the original activity. Degree of purity was monitored by isoelectric focusing in polyacrylamide gels. Some characteristics of the cathepsin were determined. The purified cathepsin hydrolyzed urea-denatured hemoglobin readily at pH 3.5, but it had no activity on substrates specific for cathepsins A, B or c. a-N-benzoyloxycarbonyl- L-gutamyl-L-tyrosine, a-N-benzoyl-L-argininamide hydrochloride and a-N-acetyl-L-tyrosinamide. Approximate isoelectric point was pH 5.6. The purified enzyme was similar to cathepsin D. Parmesan, Romano, and Cheddar cheese manufactured with lamb pregastric esterase and gastric extracts added to the curd or milk were superior in flavor when both were employed, and either extract alone made better cheese than the uninoculated control.

Pagrindinis darbo tikslas yra vadovo, kaip lyderio, bruožų analizė ir remiantis atliktu tyrimu aprašyti galimus lyderystės vystymo metodus šiuolaikinėje organizacijoje. Darbas sudarytas iš trijų dalių : teorinės analizės, situacijos analizės ir projektinių sprendimų. Atliktas vadovų, kaip lyderių bruožų skirtingo tipo organizacijose tyrimas atskleidė, jog skirtinga situacija lemia skirtingą vadovo elgesį, ir norint būti efektyviu vadovu lyderiu būtina mokėti pritaikyti skirtingus valdymo metodus skirtingose situacijose. Savo ruožtu, kiekviena situacija organizacijoje reikalauja skirtingų vadovo kompetencijų ir savybių pritaikymo, kurias reikia nuolat ugdyti ir tobulinti, net ir sėkmingai vykdant veiklą. Trečiojoje darbo dalyje pateikėme metodus ir priemones ugdyti lyderio savybes naudojant ugdančiojo vadovavimo metodiką. / The main target of this diploma paper is analysis of skills of manager as a leader and basing on investigation results to provide possible measures and tools to improve leadership skills in contemporary organisation. There are three parts of diploma paper: theoretical analysis, research and solutions proposal part. The investigation of manager skills of different type of organisation displayed that different situation needs different management skills, and to be effective leader it is needed to adapt skills according to situation. Only when leaders come to see themselves as incomplete – as having boh strenghs and weakness, then they will be able to improve their skills. The points for improvement we had provided in tha last part of our diploma paper, which we found the most important after the analysis that we have made.

The aim of this essey is to find out to what extend reservation affects developement of the countryside and to see how the citizens themselves percieve the landmark protection and what advantages and disadvantages they think it brings to them. Another aim was to find out relations between the villages council and landmark protection authorities in Mělník district.

According to a study made at Linköping University, there are few options besides self-catered food and buying food at a restaurant. According to the same survey, a majority of the students seek further options in the market. A proposed solution, tested here, is to develop a platform for selling and buying homemade food through a web application. Development takes place in iterations where design and functionality are supported by scientific theory. Changes are based on qualitative opinions from user tests at the end of each iteration. The work is specifically aimed at creating a design with associated features that allow quick navigation to the desired transaction while the user experiences a good navigability. The balance between navigability and time aspects has been central to the report and influenced all aspects of the web application's design. The report shows that it is possible to develop a web application according to the above criteria and identify what is essential for the users. / Enligt en undersökning vid Linköpings universitet finns det få alternativ till egenlagad mat och att köpa mat på restaurang. Enligt samma undersökning eftersträvar en majoritet av studenterna ett ytterligare alternativ på marknaden. En föreslagen lösning, som testas här, är att genom utvecklingen av en webbapplikation skapa en plattform för försäljning och köp av hemlagad mat. Utveckling sker i iterationer där design och funktionalitet underbyggs med vetenskaplig teori. Förändringar baseras på kvalitativa åsikter från användartester i slutet av varje iteration. Arbetet är speciellt inriktat på att skapa en design med tillhörande funktioner som tillåter en snabb navigering till den önskade transaktionen samtidigt som användaren upplever en god navigerbarhet. Avvägningen mellan navigerbarhet och tidsaspekter har varit centralt i rapporten och påverkat samtliga delar av webbapplikationens utformning. Rapporten visar att det är möjligt att utveckla en webbapplikation efter ovan nämnda kriterier och identifierar vad som upplevs centralt för användarna.

The theme of this diploma thesis is employee training and developement in the company Mondi Štětí PLC. The aim of the diploma thesis is to analyze the system of the employee training and developement, the evaluation of the conducted analysis and to design potential recommendations which causes to improve the whole system of the employee training and developement. The theoretical part defines basic terms in the field of the employee training and developement. It describes the process of the systematic employee training and developement, commonly used training methods, the process of the managers development and it mentions some modern trends of training. The practical part contains the brief description of the company, the analysis of present system of the employee training and developement and the evaluation results of the conducted analysis. The final part contains designs and recommendations to improve the process of the employee training and developement and to contribute to prosperity of the whole company.

This essay will exlopre the character of one of our times most beloved fictional characters, Harry Potter. The purpose of this esay is to investigate Harry's character in J.K. Rowling's first novel and to show that it is his his character that is one of the chief reasons for the popularity of the book, and indeed, of the entire series. The various different passages in Harry's role are well-illustrated by close reading of the significant passages in different parts of the novel.

The project is a solution of the Moravske square public space.

Le foncier économique contribue activement au développement des entreprises sur les territoires en tant que traduction spatiale de l’activité économique et que forme d’intervention des acteurs publics en France, et dans plusieurs pays européens.La localisation des activités économiques se définit en économie spatiale en fonction de la distance au centre urbain, fictif ou réel. La valeur que les agents économiques accordent à une localisation s’établit à partir du niveau de rente différentielle perçue par les propriétaires fonciers. Cette rente est une fonction décroissante de la distance au centre urbain qui varie selon les différentes activités (logements, bureaux, commerces, industries). La concentration des activités dépendrait des mécanismes de rente foncière et résulterait d’un arbitrage entre proximité et distance au centre que réaliseraient les entreprises. Cependant, ces explications sont incomplètes à nos yeux pour expliquer les trajectoires économiques divergentes entre les territoires. En économie spatiale, le foncier devient une variable d’ajustement pour l’entreprise liée au niveau de rente foncière supporté. Cette approche perd néanmoins de sa pertinence lorsque les entreprises tirent le développement économique d’un territoire sans conduire à des niveaux de rente foncière élevée. Ainsi, la concentration des activités économiques et la rente foncière comme mode de valorisation d’une localisation optimale ne fournissent pas des explications satisfaisantes. Le modèle explicatif y est à la fois dépendant de la disponibilité des données, de l’homogénéité de l’espace et du comportement modélisé des agents économiques.Afin d’apporter des explications nouvelles, nous avons mobilisé d’autres approches théoriques et des méthodes davantage empiriques afin d’analyser les mécanismes à l’œuvre sur des territoires variés et moyennement denses, où le foncier est pourtant très convoité par les investisseurs privés. Ces territoires sont ceux de la Plaine Saint-Exupéry, espace d’interface métropolitain de la métropole lyonnaise, d’une part, et de métropole Savoie, espace de plaines habitées entre Genève et Grenoble, d’autre part.En faisant appel à la notion de ressource territoriale nous utilisons les apports de l’économie territoriale pour comprendre les mécanismes qui relient le foncier et les activités économiques. La ressource foncière territoriale met en évidence des choix de localisation d’activités tertiaires et industrielles. Ceux-là sont structurés par des coopérations dans lesquelles interviennent les acteurs de l’aménagement et de la promotion immobilière, aux côtés des collectivités territoriales et des entreprises. Celles-ci recherchent des écosystèmes favorables au développement de leur activité et porteurs de proximité, institutionnelle et géographique.La ressource territoriale souligne le rôle des actifs spécifiques et de la coordination, ainsi que les conditions foncières du développement. La constitution de stratégies foncières, et les modes de coordination entre acteurs publics et privés qui en dépendent, explicitent les fonctions de redéployabilité et d’interface du foncier. Ces deux fonctions désignent, d’une part, le fait que la ressource foncière facilite la mutation des activités sur le territoire, et, d’autre part, que le foncier est une condition d’existence d’autres ressources pour les activités sur ce même territoire. Sans interface, des terrains seront affectés à des fonctions sans rapport avec les actifs spécifiques du territoire. Sans redéployabilité, le foncier sera commercialisé sans considération pour sa valeur réelle de long terme mais uniquement pour sa valeur marchande de court terme. À partir d’observations empiriques sur la Plaine de Saint-Exupéry et sur Métropole Savoie, nous illustrons ces notions et montrons que la maîtrise publique du foncier économique révèle la ressource foncière territoriale à l’heure où les territoires doivent inventer leur propre mode de développement pérenne. / Economic land contributes actively to the development of companies in the territories as a spatial translation of economic activity and as a form of intervention by public bodies in France and in several European countries.The location of economic activities is defined in spatial economics according to the distance to the urban center. The value that economic agents attribute to a central or peripheral location is based on the level of differential rent perceived by landowners. This income, assimilated to the land use cost incorporated in property prices, is a decreasing function of distance to the urban center, which varies according to the different activities (housing, office, retail, industry). The concentration of activities depends on the mechanisms of urban land rent and explains the differences in economic wealth between areas with high concentration of activities and jobs and spaces with low density. The land rent achieved by landowners will result in a trade-off between proximity and distance to the center that the companies would achieve through the market law. However, these explanations are incomplete to explain the divergent economic paths between territories. In spatial economics, land use is adjusted by the firm according to the level of land use they can put up with. However, this approach lacks of relevance when firms derive economic development from a territory without leading to high land rents or when development becomes a strategic issue for the territory. Thus, the concentration of economic activities and land rent are considered as a way to explain optimal locations, but they do not explain all kind of situations. The explanatory model is both dependent on the availability of data, the homogeneity of space and the modeled behavior of economic agents.In order to provide new explanations, we offer an other theoretical approach that is less econometric and more empirical in order to analyze the mechanisms for moderately dense territories where land is highly coveted by private investors. These territories are those of the Plaine de Saint-Exupéry, a metropolitan interface area of ​​the Lyon urban zone, and Metropole Savoie, an area of inhabited plains between Geneva and Grenoble.Using the notion of territorial resource we consider territorial economy to understand the relationships between land and economic activities. The territorial land resource highlights choices of localization for services and industrial activities. These are structured by cooperative relations involving actors in land development and real estate development, alongside local authorities and companies that seek for local ecosystems that are conducive to the development of their business. They may also seek for institutional and geographical proximity, which are different from the concentration of activities.The territorial resource emphasizes the diversity of development models and specific assets. The development of land use strategies, and the methods of coordination between public and private actors that depend on them, explain the functions of redeployment and interface of land. These two functions refer, on the one hand, to the fact that the land use resource facilitates the transformation of activities on the territory and, on the other hand, that land is a condition for the existence of other resources for activities on this territory. In other words, without an interface, land can be allocated to functions unrelated to the specific assets of the territory. Without redeployability, land will be marketed without consideration for its long-term value, but only for its short-term market value. Based on empirical observations on the Plaine de Saint-Exupéry and on Metropole Savoie, we illustrate these notions and show that public management over economic land use, in particular, makes it possible to reveal territorial land resources at a time when the territories have to invent their own sustainable development path.

Résumé Dissertation analysis raising of phenomenon of automobilism in the american city of Detroit in first half of 20. century. Analysis is focus primarily on the "Big Three" of car producers: Ford Motor Company, General Motors a Chrysler LLC. Analysis is temporalily delimitated with two moments, closely conected with developing of automobilism: Increase of popularity of cars at the begin of 20. century and Great depression. Important moments in delimitated time period were especially: founding of Ford Motor Company in 1903, founding of General Motors in 1908, implementation of assembly line by Henry Ford in 1913, founding of Chrysler LLC in 1925 and of course the Great Depresion from 1929. Special priority is focus on the personality od Henry Ford and his style of organization of work, company development and inovations, that were introduced mostly by his company, followed by others. Henry Ford has absolutely special position, mostly because of his complex care of his employes.Objective of this work is not only to analyze unprecedent increase and development of the city of Detroit, but also to describe demographical change of society, due by the development of automobile industry in the state of Michigan. Major question is: How the city of Detroit was changed in connection with industrial development,...

Goda möjligheter till rörelse och medveten motorisk träning skapar goda möjligheter för barn att utveckla motoriska färdigheter, vilket kan leda till bättre skolprestationer. Forskning visar att dagens stillasittande aktiviteter så som tv, surfplattor och datorer m.m. har negativ inverkan på hälsan och kan leda till en rad olika negativa hälsoeffekter. Denna studie fokuserar på pedagogers medvetenhet, syn och kunskap angående barns motoriska utveckling samt hur de arbetar för att stimulera förskolebarnen till att utveckla goda motoriska färdigheter. Metoden som använts i undersökningen är semistrukturerade intervjuer med sex stycken förskollärare. Deras svar har behandlats i studiens i resultat- och analysdel. Tidigare forskning har visat att motorisk träning i tidig ålder ger en god hälsa och bra förutsättningar för kognitiv inlärning. Motoriken anses ha betydelse får såväl motoriska som sociala färdigheter samt en god självkänsla. Resultatet i den här studien visar att det skiljer sig ganska mycket mellan de olika förskolorna och att mycket hänger på pedagogens ambitionsnivå och intresse för just motorisk utveckling. Det framkommer att några av pedagogerna jobbar aktivt och medvetet med motoriska övningar, medan andra anser att barnen automatiskt får mycket motorisk träning på förskolan genom exempelvis utegården. Förskolegården och skogen lyfts fram som betydelsefulla platser för motorisk träning enligt många av förskollärarna. Det framkommer även att flera utav förskollärarna anser att det finns för lite kunskap kring ämnet motorik i förskolan. I den här studiens diskussionsdel diskuteras resultatet i förhållande till tidigare forskning. / A good possibility for movement and conscious motor training creates opportunities for children to develop motor skills, which can lead to better school performance. Previous research shows that today's sedentary activities such as TV, tablets and computers etc. have negative impact on health and may lead to a variety of adverse health effects. This study focuses on finding out what grade of awareness, vision and knowledge preschool teachers have about child motor development, and how they work to stimulate the children in pre-school so that they develop good motor skills. The method used in the study is semi-structured interviews with six pre-school teachers. Their answers will be processed in the results and analysis section of the work. Previous research has shown that motor training at an early age gives good health and good opportunities for cognitive learning. Motor skills are considered pertinent in order to develop both social skills and a good self-esteem. The results of this study show that there are big differences between the various pre-schools and much depends on the pre-school teachers level of ambition and interest in the development of motor skills. It appears that some of the teachers are working actively and consciously with motor exercises, while others believe that children automatically get a lot of motor skill training in preschool through, for instance, the preschool playground. The pre-school playground and the forest were pointed out as important sites for motor training by many of the preschool teachers. It also reveals that several pre-school teachers believe that the knowledge of motor skills in pre-schools is small. In the last part of this study the results are discussed in relation to previous research.