Muitas plantas apresentam crescimento indeterminado e são capazes de produzir novos órgãos e tecidos ao longo de todo seu ciclo de vida. Essa capacidade é devida parcialmente à expressão altamente regulada de genes específicos, tais como os genes SQUAMOSA Promoter-Binding Protein-Like (SPLs). SPLs codificam fatores de transcrição específicos de plantas que desempenham papéis importantes em diferentes aspectos do desenvolvimento, tais como mudança de fase juvenil-adulto, definição da arquitetura da planta e amadurecimento do fruto. A maioria dos genes SPLs são pós-transcricionalmente regulados pelo microRNA (miRNA) miR156. Apesar de alguns aspectos regulados pelas SPLs serem bem estudados, suas funções moleculares durante o desenvolvimento do fruto são pouco compreendidas. Neste trabalho, nós geramos 22 eventos transgênicos de Solanum lycopersicum cv. Micro-Tom (MT) superexpressando o precursor AtMIR156b. Plantas transgênicas exibiram morfologia foliar e floral anormal além de alteração na arquitetura vegetal. Interessantemente, a maioria dos eventos apresentou frutos de crescimento indeterminado, os quais não apresentam sementes sendo caracterizados pelo crescimento de frutos secundários além da presença de estruturas vegetativas e meristemas florais ectópicos. Fazendo uso da técnica de RT-qPCR, nós encontramos uma robusta correlação entre expressão do miR156 e o fenótipo dos frutos, sugerindo que esta rota regula o desenvolvimento e determinação do fruto de tomateiro. O mutante de tomateiro Mouse ears (Me) apresenta um fraco nível de indeterminação no fruto, sendo que os níveis do transcrito miR156 maduro são maiores no fruto mutante quando comparado aos controles MT, mas significativamente menores que nos frutos transgênicos. Oito SPLs de tomateiro foram silenciadas em diferentes níveis em frutos de diferentes eventos transgênicos e no mutante Me. O fator de transcrição do tipo MADS-box MACROCALYX (MC), o qual é ortólogo ao gene APETALA1 (AP1) de arabidopsis (alvo direto in vivo da proteína SPL3), afeta a determinação da inflorescência e o desenvolvimento das sépalas. A expressão de MC foi severamente reduzida nos frutos dos eventos transgênicos, mas não no mutante Me. Este dado sugere que a desregulação da expressão de MC deve ser responsável pelo forte fenótipo de indeterminação do fruto observados nos eventos transgênicos. Tomados em conjunto, nossos dados sugerem uma nova função para a via genética mir156/SPL na regulação do desenvolvimento e determinação de frutos carnosos, provavelmente através da regulação da expressão de MC. / Many plants have indeterminate growth and are capable of producing new organs and tissues throughout their life. This capability is partially due to the highly regulated expression of specific genes such as SQUAMOSA Promoter-Binding Protein-Like (SPL) genes. SPLs encode plant-specific transcription factors that play important roles in development, such as phase transition, plant architecture, and fruit ripening. Most SPL genes are post-transcriptionally regulated by the microRNA (miRNA) miR156. Although some developmental aspects regulated by SPLs have been well studied, their molecular roles during fruit development are poorly understood. In this work, we generated 22 transgenic events of Solanum lycopersicum cv. Micro-Tom (MT) overexpressing AtMIR156b precursor. Transgenic plants exhibit abnormal leaf and flower morphology and altered vegetative architecture. Interestingly, most events display navel-like fruits that are seedless and characterized by the growth of secondary fruits and present leaf-like structures as well as ectopic flowering meristems. By using RT-qPCR, we found a robust correlation between miR156/SPL expression and the fruit phenotype, suggesting that this pathway regulates tomato fruit development and determinacy. The tomato mutant Mouse ears (Me) displays a weak level of fruit indeterminacy. Levels of mature miR156 transcripts are higher in fruits from the mutant as comparing to MT fruits, but significantly lower than in transgenic fruits. Eight tomato SPLs were downregulated at variable levels in fruits from distinct transgenic events and Me plants. The MADS-type of transcription factor Macrocalyx (MC) gene is an orthologue of Arabidopsis AP1 (a direct in vivo target of SPL3) and affects tomato inflorescence determinacy and sepal development. MC was severely downregulated in fruits from transgenics but not in fruits from Me mutant. This data suggests that the MC misregulation may lead to the strong fruit indeterminacy phenotype observed in the transgenic events. Taken together, our data suggest a new function for miR156/SPL pathway in regulating fruit development and determinacy likely through the regulation of MC expression.

Muitas plantas apresentam crescimento indeterminado e são capazes de produzir novos órgãos e tecidos ao longo de todo seu ciclo de vida. Essa capacidade é devida parcialmente à expressão altamente regulada de genes específicos, tais como os genes SQUAMOSA Promoter-Binding Protein-Like (SPLs). SPLs codificam fatores de transcrição específicos de plantas que desempenham papéis importantes em diferentes aspectos do desenvolvimento, tais como mudança de fase juvenil-adulto, definição da arquitetura da planta e amadurecimento do fruto. A maioria dos genes SPLs são pós-transcricionalmente regulados pelo microRNA (miRNA) miR156. Apesar de alguns aspectos regulados pelas SPLs serem bem estudados, suas funções moleculares durante o desenvolvimento do fruto são pouco compreendidas. Neste trabalho, nós geramos 22 eventos transgênicos de Solanum lycopersicum cv. Micro-Tom (MT) superexpressando o precursor AtMIR156b. Plantas transgênicas exibiram morfologia foliar e floral anormal além de alteração na arquitetura vegetal. Interessantemente, a maioria dos eventos apresentou frutos de crescimento indeterminado, os quais não apresentam sementes sendo caracterizados pelo crescimento de frutos secundários além da presença de estruturas vegetativas e meristemas florais ectópicos. Fazendo uso da técnica de RT-qPCR, nós encontramos uma robusta correlação entre expressão do miR156 e o fenótipo dos frutos, sugerindo que esta rota regula o desenvolvimento e determinação do fruto de tomateiro. O mutante de tomateiro Mouse ears (Me) apresenta um fraco nível de indeterminação no fruto, sendo que os níveis do transcrito miR156 maduro são maiores no fruto mutante quando comparado aos controles MT, mas significativamente menores que nos frutos transgênicos. Oito SPLs de tomateiro foram silenciadas em diferentes níveis em frutos de diferentes eventos transgênicos e no mutante Me. O fator de transcrição do tipo MADS-box MACROCALYX (MC), o qual é ortólogo ao gene APETALA1 (AP1) de arabidopsis (alvo direto in vivo da proteína SPL3), afeta a determinação da inflorescência e o desenvolvimento das sépalas. A expressão de MC foi severamente reduzida nos frutos dos eventos transgênicos, mas não no mutante Me. Este dado sugere que a desregulação da expressão de MC deve ser responsável pelo forte fenótipo de indeterminação do fruto observados nos eventos transgênicos. Tomados em conjunto, nossos dados sugerem uma nova função para a via genética mir156/SPL na regulação do desenvolvimento e determinação de frutos carnosos, provavelmente através da regulação da expressão de MC. / Many plants have indeterminate growth and are capable of producing new organs and tissues throughout their life. This capability is partially due to the highly regulated expression of specific genes such as SQUAMOSA Promoter-Binding Protein-Like (SPL) genes. SPLs encode plant-specific transcription factors that play important roles in development, such as phase transition, plant architecture, and fruit ripening. Most SPL genes are post-transcriptionally regulated by the microRNA (miRNA) miR156. Although some developmental aspects regulated by SPLs have been well studied, their molecular roles during fruit development are poorly understood. In this work, we generated 22 transgenic events of Solanum lycopersicum cv. Micro-Tom (MT) overexpressing AtMIR156b precursor. Transgenic plants exhibit abnormal leaf and flower morphology and altered vegetative architecture. Interestingly, most events display navel-like fruits that are seedless and characterized by the growth of secondary fruits and present leaf-like structures as well as ectopic flowering meristems. By using RT-qPCR, we found a robust correlation between miR156/SPL expression and the fruit phenotype, suggesting that this pathway regulates tomato fruit development and determinacy. The tomato mutant Mouse ears (Me) displays a weak level of fruit indeterminacy. Levels of mature miR156 transcripts are higher in fruits from the mutant as comparing to MT fruits, but significantly lower than in transgenic fruits. Eight tomato SPLs were downregulated at variable levels in fruits from distinct transgenic events and Me plants. The MADS-type of transcription factor Macrocalyx (MC) gene is an orthologue of Arabidopsis AP1 (a direct in vivo target of SPL3) and affects tomato inflorescence determinacy and sepal development. MC was severely downregulated in fruits from transgenics but not in fruits from Me mutant. This data suggests that the MC misregulation may lead to the strong fruit indeterminacy phenotype observed in the transgenic events. Taken together, our data suggest a new function for miR156/SPL pathway in regulating fruit development and determinacy likely through the regulation of MC expression.

Human hearing is to collect information from the outside world and is one of the basic human senses. Part of this thesis is devoted to acoustics, properties of the human ear in terms of perception of sounds and methods, which human ears are investigating. Audiometer is an instrument which is most often used in healthcare. The main content of this thesis is to design pure tone audiometer, which is important for the subjective tests of human hearing non-invasive method.

No description available.

This study focuses on the novels of Quicksand by Nella Larsen and Invisible Man by Ralph Ellison to explore the phenomenon of poqéakh (פֹּקֵחַ) through the fictionalized lived experiences of their protagonists, Helga Crane and invisible man. Each novelist’s representation of poqéakh offers a portrait of the protagonists’ psyches. The narratives reveal an unsettling truth for the protagonists, who are members of a population often targeted, stigmatized, and fashioned or re-fashioned by Americans and various environs in American society, that they must assimilate—not only their bodies, but their psyches too to fit the “white man’s pattern” (Larsen 4). Their realities inform them that non-conformity and/or developing or utilizing their intellect is disadvantageous—perceiving is unfavorable. Each protagonist learns that she and he will not only be limited by their imaginations or abilities, but also by persons and constructs within American society keeping them witless and amenable. The environs presented in forms such as schools, jobs, even people who prepare each protagonist to accept all and any disparity (inequality and inequity), they are made to be persistently and surreptitiously instructive. As such, these environs are always educating (or training), always molding the psyches of the protagonists to live within a frame—the construct (American society). These ever informing boundaries thoroughly acquaint each protagonist on “how to scale down [their] desires and dreams so that they will come within reach of possibility” (Thurman 115). Poqéakh leads Helga Crane to perceive the boundaries while it prevents the invisible man from returning to unblissful ignorance, thus, for him, providing momentary periods of lucidity. This study utilizes a qualitative research design and method, and relies on phenomenological theory to successfully analyze the novels and explicate on the representations of poqéakh. As this study will illustrate, Larsen and Ellison offer as representative via their novels two narratives of the diasporic psyche (mind), wherein their protagonists’ experiences of poqéakh lead to some unmitigated facts and disturbing truths about their reality.

Made available in DSpace on 2016-01-26T12:51:55Z (GMT). No. of bitstreams: 1 josealvarogasques_tese (1).pdf: 475777 bytes, checksum: 596f8b3f62d5b000afd77aab0e98e39b (MD5) Previous issue date: 2004-02-05 / Objective: To appraise the psychosocial effects of otoplasty on children suffering from prominent ears. Casuistic and Method: A total of 15 male and 15 female patients were studied, with ages ranging from 6 to 14 years, clinical diagnosis of prominent ears and complaints related to the presence of this deformity. Pre-operative social competence and behavioral disturbance tests were applied to the children s guardians and teachers. Additionally, the inventory of anxiety trait-state for the child (Idate C) and the child depression inventory (CDI) were employed, as was a self-image study using drawings. Six months after corrective otoplasty by the Converse technique used by Tanzer, all the children were re-assessed. The statistical analysis was performed for pure scores in the descriptive phase using basic statistics, the mean, standard deviation, median and percentages. To compare the pre- and post-operative phases, the non-matched indication test of the median was used. Results: When the CBCL was applied to guardians, there were reductions in the anxiety and depression, aggressiveness and attention, thinking and social deficits. There were also improvements in both school and out-of-school activities. When the CBCL was applied to the teachers, reductions in anxiety and depression, aggressiveness, attention and social deficits, somatic complications and impulsiveness were observed. Improvements in academic performance, stable work, learning and happiness were also evidenced. Comparing the anxiety state assessed in the pre- and post-operative periods using Idate C, we observed that 18 had a reduction in anxiety in the post-operative period, 2 remained the same and 7 children had an increase. Comparing the anxiety traits of the children, we verified that in 8 the anxiety diminished, 14 had no changes and in 5 the anxiety trait increased in the post-operative period. Analysis using CDI demonstrated that all patients with moderate pre-operative depression suffered from mild depression after the surgery. In relation to the drawings in the pre- and post-operative periods, we noted that a new somatic image was formed, creating a new sensorial perception of the internal world and a better adaptation to the self and to others (relations, environments). The surgical technique used for the correction of protruding ears, otoplasty, is recognized and employed in several specialist services giving harmonious and satisfactory anatomical results in the post-operative period of all patients. / Objetivo: avaliar os efeitos psicossocias da otoplastia em crianças portadoras de orelhas proeminentes. Casuística e método: foram estudados 30 pacientes, 15 do sexo masculino e 15 do feminino, com idade entre 06 e 14 anos, com diagnóstico clínico de orelhas em abano, e queixas relativas à presença da deformidade. Utilizados no pré-operatório testes que avaliaram competência social e transtornos comportamentais - (CBCL) aplicado aos pais ou responsável e ao professor das crianças, aplicado o inventário de ansiedade traço-estado para criança (Idate C) e o inventário de depressão para crianças (CDI). Também utilizamos o estudo de auto-imagem, através de desenhos. após 6 meses da otoplastia, pela técnica de Converse aplicada por Tanzer, foram replicados os testes e colhidos os desenhos. A análise estatística foi efetuada para os escores puros, na fase descritiva por meio de estatísticas básicas: média, desvio padrão, mediana e percentuais. Para comparação das fases pré e pós-operatória, foi utilizado o teste não paramétrico do sinal para a mediana. Resultados: No CBCL aplicados aos pais ou responsável, houve diminuição da ansiedade e depressão, da agressividade, dos problemas de atenção, de pensamento e sociais; aumento nas atividades escolares e extra. No CBCL aplicado ao professo, observamos diminuição da ansiedade e depressão, da agressividade, dos problemas sociais e de atenção, nas compliacações somáticas e na impulsividade; aumento no desempenho acadêmico, trabalho firme, aprendizado e alegria. Aplicando Idate C: Comparando a ansiedade estado na fase pré com pós-operatória, verificamos que 18 diminuíram a evidência de ansiedade estado no pós-operatório, 2 permaneceram iguais e 7 crianças aumentaram. Comparando ansiedade traço na fase pré com pós-operatória: verificamos que 8 diminuíram a evidência de ansiedade traço no pós-operatório, 14 permaneceram semelhantes e 5 crianças aumentaram. O CDI mostrou um desaparecimento completo dos pacientes com depressão Nota de Resumo moderada no pós-operatório, transformando em depressão leve. Com relação aos desenhos no pré e pós-operatório, notamos que com uma nova imagem somática formada, criou-se uma nova percepção sensorial do mundo interno e uma melhor adequação ao self (eu) e ao outro (relações, ambientes). A técnica cirúrgica aplicada para a correção da orelha em abano é de conhecimento e aplicabilidade em vários serviços da especialidadem, trazendo-nos resultados anatômicos harmônicos e satisfatórios no pós-operatório de todos os pacientes.

L’analyse du vocabulaire grec du visage dans l’ensemble des romans de Chariton, de Xénophon, de Longus, d’Héliodore et d’Achille Tatius a pour dessein de montrer l’intérêt certain, à la fois esthétique et sensoriel, porté à cette partie souveraine du corps. Quelle est donc l’importance accordée au visage du héros ou de l’héroïne ? Et de quelle manière le discours rend-il compte de son incarnation, de sa réalité organique ? Comment les visages des personnages interagissent-ils ? Parce que le visage se révèle une interface entre l’intime et le social, entre l’intériorité et l’expressivité, on peut se demander en quoi ce lieu privilégié du corps, à travers la description de l’aspect physique des personnages, caractérise leur éthos permanent ou communique au lecteur leurs émotions fugitives. Le visage s’offre aux regards et interpelle. Ses traits sont autant de signes à interpréter pour celui ou celle qui le regarde et dont il mobilise le système de reconnaissance et de représentation. Certes, la description physique des héroïnes comme celle des jeunes hommes ne se limite pas au visage. Mais, seul le visage, qui n’a rien d’incertain, d’irrégulier, de disharmonieux, est appelé à refléter les vertus des personnages mais aussi ses plus grandes souffrances. La mise en icônes de traits représentatifs des personnages s’inscrit dans la logique des procédures de description physique qui caractérise la culture romanesque. Le roman aime ainsi à représenter la beauté, en alliant aux manifestations physiques les émotions de l’âme. Les visages des héros romanesques grecs sont dévoilés dans une sorte de mosaïque à la fois anatomique et littéraire, évoquant les éléments fondamentaux qui les constituent. Ainsi, sans confondre visage et portrait, nous avons déconstruit le visage romanesque pour en montrer les diverses facettes, la palette des couleurs, les références littéraires intertextuelles et mythologiques mais aussi certains invariants, pour enfin mieux le reconstruire. Nous avons donc procédé à l’étude et à l’analyse du visage, non seulement comme entité mais en tant que visage morcelé, voire éclaté. L’étude approfondie des sens s’est attachée à souligner la passion, ses effets et les émotions du corps, entre plaisir et souffrance, entre affection et violence. Cette recherche a permis de souligner les éléments communs aux différents romanciers, mais aussi leur originalité d'écriture. L'importance accordée au visage et plus généralement au corps dans la narratologie laisse apparaître le reflet des valeurs de la société grecque de leur temps. / The analysis of Greek vocabulary about the face in Chariton, Xenophon, Longus, Heliodorus and Achilles Tatius’s novels as a whole plans to show the definite interest, both aesthetic and sensory focused on this sovereign part of the body. So what is the importance attached to the hero or heroine’s faces? And how does the discourse explain its incarnation and organical reality? The face proves to be an interface between the private and social world, between interiority and expressiveness. So we can wonder how this privileged part of the body characterizes their permanent ethos ; we can wonder how it transmits their fleeting emotions to the reader, through the description of the physical look of the characters. The face catches attention. Its features mobilize the system of recognition and representation. Indeed the physical description of heroines as well as heroes is not limited to the face. But only the face, with nothing uncertain, irregular, disharmonious, is assigned to reflect the characters’ virtues but also their greatest suffering. « La mise en icônes »of characters’ representative features is part of the procedures of physical description that characterize the culture of the novel. Thus the novel likes to represent beauty by combining physical expressions with soul feeling. The faces of Greek novelistic heroes are revealed in a kind of mosaic at once anatomical and literary, evoking the basic elements that constitute them. Thus, without mixing up face and portrait, we have deconstructed the novelistic face to show its various facets, colour palette, intertextual literary and mythological references ; but also to show some invariants to, at last, rebuild it in a better way. We have therefore conducted a thorough study and analysis of the face not only as an entity but as a fragmented even blown up face. The detailed study of senses has endeavoured to emphasize passion and its effects, and show the emotions of the body between pleasure and suffering, affection and violence. On the one hand this research has permitted to highlight the elements common to the different novelists, their original writing and the importance granted to face and more generally to body in narratology. On the other hand it has led us to analyze the reflection of the values of the Greek society of their days.

The theses presents results of experiments with spring barley started in years 2017 and 2018. The experiments were put into practice at Mr Jiří Nepovím's (BSc) place in Opatovice near Světlá nad Sázavou. Laudis 550 was used as the experimental variety of spring barley and the objective of the experiment was to observe the increasing amount of nitrogen and its effect on the resulting quantity and quality. The experimental amounts of nitrogen were set to 60 kg.ha-1, 75 kg.ha-1, 90 kg.ha-1, 105 kg.ha-1 and 120 kg.ha-1. With the increasing amount of nitrogen the crop increased of 0,2 - 0,4 t.ha-1. The share of nitrogen substances in grain increased of 0,2-0,5 %.