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Showing 81 to 88 of 88 (0.022 seconds)| 81 |
Interactions of FCHo2 with lipid membranesChwastek, Grzegorz 06 February 2013 (has links)
Endocytosis is one of the most fundamental mechanisms by which the cell communicates with its surrounding. Specific signals are transduced through the cell membrane by a complex interplay between proteins and lipids. Clathrin depended endocytosis is one of important signalling pathways which leads to budding of the plasmalemma and a formation of endosomes. The FCHo2 is an essential protein at the initial stage of the this process. In is a membrane binding protein containing BAR (BIN, Amphiphysin, Rvs) domain which is responsible for a membrane binding. Although numerous valuable work on BAR proteins was published recently, the mechanistic description of a BAR domain functionality is missing. In present work we applied in vitro systems in order to gain knowledge about molecular basis of the activity of the FCHo2 BAR domain. In our studies we used supported lipid bilayers (SLBs) and lipid monolayers as s model membrane system.
The experiments were carried out with a minimal number of components including the purified FCHo2 BAR domain. Using SLBs we showed that the BAR domain can bind to entirely flat bilayers. We also demonstrated that these interactions depend on the negatively charged lipid species incorporated in the membrane. We designed an assay which allows to quantify the membrane tubulation. We found out that the interaction of the FCHo2 BAR domain with the lipid membrane is concentration dependent. We showed that an area of the bilayer deformed by the protein depends on the amount of the used BAR domain.
In order to study the relation between the mobility of lipids and the activity of FCHo2 BAR domain we designed a small-volume monolayer trough. The design of this micro-chamber allows for the implementation of the light microscopy. We demonstrated that the measured lipid diffusion in the monolayer by our new approach is in agreement with literature data. We carried out fluorescence correlation spectroscopy (FCS) experiments at different density of lipids at the water-air interface.We showed that the FCHo2 BAR domain binding affinity is proportional to the mean molecular area (MMA). We additionally demonstrated that the increased protein binding is correlated with the higher lipid mobility in the monolayer.
Additionally, by curing out high-speed atomic force microscopy (hsAFM) we acquired the structural information about FCHo2 BAR domains orientation at the membrane with a high spatio-temporal resolution. Obtained data indicate the BAR domains interact witheach other by many different contact sites what results in a variety of protein orientations in a protein assemble.
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Hot Brownian MotionRings, Daniel 18 February 2013 (has links) (PDF)
The theory of Brownian motion is a cornerstone of modern physics. In this thesis, we introduce a nonequilibrium extension to this theory, namely an effective Markovian theory of the Brownian motion of a heated nanoparticle. This phenomenon belongs to the class of nonequilibrium steady states (NESS) and is characterized by spatially inhomogeneous temperature and viscosity fields extending in the solvent surrounding the nanoparticle.
The first chapter provides a pedagogic introduction to the subject and a concise summary of our main results and summarizes their implications for future developments and innovative applications.
The derivation of our main results is based on the theory of fluctuating hydrodynamics, which we introduce and extend to NESS conditions, in the second chapter. We derive the effective temperature and the effective friction coefficient for the generalized Langevin equation describing the Brownian motion of a heated nanoparticle. As major results, we find that these parameters obey a generalized Stokes–Einstein relation, and that, to first order in the temperature increment of the particle, the effective temperature is given in terms of a set of universal numbers.
In chapters three and four, these basic results are made explicit for various realizations of hot Brownian motion. We show in detail, that different degrees of freedom are governed by distinct effective parameters, and we calculate these for the rotational and translational motion of heated nanobeads and nanorods. Whenever possible, analytic results are provided, and numerically accurate approximation methods are devised otherwise.
To test and validate all our theoretical predictions, we present large-scale molecular dynamics simulations of a Lennard-Jones system, in chapter five. These implement a state-of-the-art GPU-powered parallel algorithm, contributed by D. Chakraborty. Further support for our theory comes from recent experimental observations of gold nanobeads and nanorods made in the the groups of F. Cichos and M. Orrit. We introduce the theoretical concept of PhoCS, an innovative technique which puts the selective heating of nanoscopic tracer particles to good use.
We conclude in chapter six with some preliminary results about the self-phoretic motion of so-called Janus particles. These two-faced hybrids with a hotter and a cooler side perform a persistent random walk with the persistence only limited by their hot rotational Brownian motion. Such particles could act as versatile laser-controlled nanotransporters or nanomachines, to mention just the most obvious future nanotechnological applications of hot Brownian motion.
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Darstellung und Verwendung von Nucleolipiden zur Lipophilisierung von Nucleinsäuren sowie deren Wechselwirkung und Duplex-Bildung an horizontalen Lipid-Bilayers und Phasengrenzen zur Entwicklung einer neuartigen RNA/DNA-Analytik / Synthesis and Application of Nucleolipids for the Lipophilization of Nucleic Acids and Their Interaction and Duplex Formation at Horizontal Lipid-Bilayers and Phase Boundaries for the Development of a Novel RNA/DNA AnalyticsWerz, Emma 17 February 2016 (has links)
Ziel der vorgestellten Arbeit war die Synthese von Nucleolipiden zur Lipophilisierung von Oligonucleotiden sowie deren Untersuchung im Hinblick auf ihre Wechselwirkung und Duplex-Bildung an horizontalen Lipidmembranen und verschiedenen Phasengrenzen zur Entwicklung eines neuartigen Bio-Chips für die RNA/DNA-Analyse.
Mit der Synthese N(3)-prenylierter und 2’,3’-O-ketalisierter Pyrimidinbasen Uridin und Methyluridin wurden Nucleolipid-Bausteine dargestellt, die auch als terminale Kopfgruppen eines Oligonucleotid-Dodecamers den lipophilen Charakter dieser Oligonucleotid-Sequenz erhöhten. Für den Einsatz solcher LONs (Lipo-Oligonucleotide) in einer vereinfachten RNA/DNA-Analytik wurde eine Vielzahl von Lipo-Oligonucleotiden mit diversen Nucleolipid-Kopfgruppen synthetisiert und auf ihr Einlagerungsverhalten in künstliche Lipid-Bilayer untersucht. Fluoreszenz-spektroskopische Untersuchungen zeigten, dass alle Lipo-Oligonucleotide in der Lage sind, sich in künstliche Lipid-Bilayer einzulagern. Abhängig von der Struktur, der Länge und der Anzahl der C-Atom-Ketten dieser lipophilen Anker-Bausteine wurden die Geschwindigkeit und die Festigkeit der Verankerung im Lipid-Bilayer beeinflusst.
Des Weiteren wurde die Hybridisierung von LONs mit komplementären Oligomeren an Lipidmembranen untersucht. Es konnte gezeigt werden, dass die im Bilayer verankerten Lipo-Oligonucleotide mit komplementären Oligomeren DNA-Duplexe bilden. Die hybridisierte DNA wurde nicht nur über einen kovalent gebundenen Cy5-Fluorophor am Gegenstrang nachgewiesen, sondern auch über den DNA-Interkalator SYBR Green I (SG).
Am Beispiel von zwei Lipo-Oligonucleotiden (LON 20 und 23), die sich schnell und fest in der Bilayermembran verankern, konnte eine spontane Akkumulation dieser LONs an CHCl3/H2O sowie H2O/n-Decan Grenzflächen direkt nach der Probenzugabe beobachtet werden. Diese und andere Ergebnisse stützen den Einsatz von Lipo-Oligonucleotiden als Ziel-Oligomere in einem neuartigen RNA/DNA-Nachweisverfahren an Phasengrenzen.
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Hot Brownian MotionRings, Daniel 19 December 2012 (has links)
The theory of Brownian motion is a cornerstone of modern physics. In this thesis, we introduce a nonequilibrium extension to this theory, namely an effective Markovian theory of the Brownian motion of a heated nanoparticle. This phenomenon belongs to the class of nonequilibrium steady states (NESS) and is characterized by spatially inhomogeneous temperature and viscosity fields extending in the solvent surrounding the nanoparticle.
The first chapter provides a pedagogic introduction to the subject and a concise summary of our main results and summarizes their implications for future developments and innovative applications.
The derivation of our main results is based on the theory of fluctuating hydrodynamics, which we introduce and extend to NESS conditions, in the second chapter. We derive the effective temperature and the effective friction coefficient for the generalized Langevin equation describing the Brownian motion of a heated nanoparticle. As major results, we find that these parameters obey a generalized Stokes–Einstein relation, and that, to first order in the temperature increment of the particle, the effective temperature is given in terms of a set of universal numbers.
In chapters three and four, these basic results are made explicit for various realizations of hot Brownian motion. We show in detail, that different degrees of freedom are governed by distinct effective parameters, and we calculate these for the rotational and translational motion of heated nanobeads and nanorods. Whenever possible, analytic results are provided, and numerically accurate approximation methods are devised otherwise.
To test and validate all our theoretical predictions, we present large-scale molecular dynamics simulations of a Lennard-Jones system, in chapter five. These implement a state-of-the-art GPU-powered parallel algorithm, contributed by D. Chakraborty. Further support for our theory comes from recent experimental observations of gold nanobeads and nanorods made in the the groups of F. Cichos and M. Orrit. We introduce the theoretical concept of PhoCS, an innovative technique which puts the selective heating of nanoscopic tracer particles to good use.
We conclude in chapter six with some preliminary results about the self-phoretic motion of so-called Janus particles. These two-faced hybrids with a hotter and a cooler side perform a persistent random walk with the persistence only limited by their hot rotational Brownian motion. Such particles could act as versatile laser-controlled nanotransporters or nanomachines, to mention just the most obvious future nanotechnological applications of hot Brownian motion.:1 Introduction and Overview
2 Theory of Hot Brownian Motion
3 Various Realizations of Hot Brownian Motion
4 Toy Model and Numerical Methods
5 From Experiments and Simulations to Applications
6 Conclusion and Outlook
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Novel fabrication and testing of light confinement devicesRing, Josh January 2016 (has links)
The goal of this project is to study novel nanoscale excitation volumes, sensitive enoughto study individual chromophores and go on to study new and exciting self assemblyapproaches to this problem. Small excitation volumes may be engineered using light con-finement inside apertures in metal films. These apertures enhance fluorescence emissionrates, quantum yields, decrease fluorescence quenching, enable higher signal-to-noiseratios and allow higher concentration single chromophore fluorescence, to be studied byrestricting this excitation volume. Excitation volumes are reported on using the chro-mophore's fluorescence by utilising fluorescence correlation spectroscopy, which monitorsfluctuations in fluorescence intensity. From the correlation in time, we can find the res-idence time, the number of chromophores, the volume in which they are diffusing andtherefore the fluorescence emission efficiency. Fluorescence properties are a probe ofthe local environment, a particularly powerful tool due to the high brightness (quantumyield) fluorescent dyes and sensitive photo-detection equipment both of which are readilyavailable, (such as avalanche photodiodes and photomultiplier tubes). Novel materialscombining the properties of conducting and non-conducting materials at scales muchsmaller than the incident wavelength are known as meta-materials. These allow combi-nations of properties not usually possible in natural materials at optical frequencies. Theproperties reported so far include; negative refraction, negative phase velocity, fluorescenceemission enhancement, lensing and therefore light confinement has also been proposed tobe possible. Instead of expensive and slow lithography methods many of these materialsmay be fabricated with self assembly techniques, which are truly nanoscopic and otherwiseinaccessible with even the most sophisticated equipment. It was found that nanoscaled volumes from ZMW and HMMs based on NW arrays wereall inefficient at enhancing fluorescence. The primary cause was the reduced fluorescencelifetime reducing the fluorescence efficiency, which runs contrary to some commentatorsin the literature. NW based lensing was found to possible in the blue region of the opticalspectrum in a HMM, without the background fluorescence normally associated with a PAAtemplate. This was achieved using a pseudo-ordered array of relatively large nanowireswith a period just smaller than lambda / 2 which minimised losses. Nanowires in the traditionalregime lambda / 10 produced significant scattering and lead to diffraction, such that they werewholly unsuitable for an optical lensing application.
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Nanoscale Brownian Dynamics of Semiflexible BiopolymersMühle, Steffen 16 July 2020 (has links)
No description available.
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Algorithmes de conception de lois de commande prédictives pour les systèmes de production d’énergie / Control design algorithms for Model-Based Predictive Power Control. Application for Wind EnergyNgo, Van Quang Binh 22 June 2017 (has links)
Cette thèse vise à élaborer de nouvelles stratégies de commande basées sur la commande prédictive pour le système de génération d’énergie éolienne. La topologie des systèmes de production éolienne basées sur le Générateur Asynchrone à Double Alimentation (GADA) qui convient à des plateformes de génération dans la gamme de puissance de 1.5 à 6 MW est abordée. Du point de vue technologique, le convertisseur à trois niveaux et clampé par le neutre (3L-NPC) est considéré comme une bonne solution pour une puissance élevée en raison de ses avantages: capacité à réduire la distorsion harmonique de la tension de sortie et du courant, et augmentation de la capacité du convertisseur grâce à une tension réduite appliquée à chaque semi-conducteur de puissance. Une description détaillée de la commande prédictive à ensemble de commande fini (FCS-MPC) avec un horizon de prédiction de deux pas est présentée pour deux boucles de régulation: celle liée au convertisseur connecté au réseau et celle du convertisseur connecté au GADA. Le principe de la commande repose sur l’utilisation d’un modèle de prédiction permettant de prédire le comportement du système pour chaque état de commutation du convertisseur. La minimisation d’une fonction de coût appropriée prédéfinie permet d’obtenir la commutation optimale à appliquer au convertisseur. La thèse étudie premièrement les problèmes liées à la compensation du temps de calcul de la commande et au choix et aux pondérations de la fonction de coût. Ensuite, le problème de stabilité de la commande FCS-MPC est abordé en considérant une fonction de Lyapunov dans la minimisation de la fonction de coût. Finalement, une étude sur la compensation des effets des temps morts du convertisseur est présentée. / This thesis aims to elaborate new control strategies based on Model Predictive control for wind energy generation system. We addressed the topology of doubly fed induction generator (DFIG) based wind generation systems which is suitable for generation platform power in the range in 1.5-6 MW. Furthermore, from the technological point of view, the three-level neutral-point clamped (3L-NPC) inverter configuration is considered a good solution for high power due to its advantages: capability to reduce the harmonic distortion of the output voltage and current, and increase the capacity of the converter thanks to a decreased voltage applied to each power semiconductor.In this thesis, we presented a detailed description of finite control set model predictive control (FCS-MPC) with two step horizon for two control schemes: grid and DFIG connected 3L-NPC inverter. The principle of the proposed control scheme is to use system model to predict the behaviour of the system for every switching states of the inverter. Then, the optimal switching state that minimizes an appropriate predefined cost function is selected and applied directly to the inverter.The study of issues such as delay compensation, computational burden and selection of weighting factor are also addressed in this thesis. In addition, the stability problem of FCS-MPC is solved by considering the control Lyapunov function in the design procedure. The latter study is focused on the compensation of dead-time effect of power converter.
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Methodologies for FPGA Implementation of Finite Control Set Model Predictive Control for Electric Motor DrivesLao, Alex January 2019 (has links)
Model predictive control is a popular research focus in electric motor control as it allows designers to specify optimization goals and exhibits fast transient response. Availability of faster and more affordable computers makes it possible to implement these algorithms in real-time. Real-time implementation is not without challenges however as these algorithms exhibit high computational complexity. Field-programmable gate arrays are a potential solution to the high computational requirements. However, they can be time-consuming to develop for. In this thesis, we present a methodology that reduces the size and development time of field-programmable gate array based fixed-point model predictive motor controllers using automated numerical analysis, optimization and code generation. The methods can be applied to other domains where model predictive control is used. Here, we demonstrate the benefits of our methodology by using it to build a motor controller at various sampling rates for an interior permanent magnet synchronous motor, tested in simulation at up to 125 kHz. Performance is then evaluated on a physical test bench with sampling rates up to 35 kHz, limited by the inverter. Our results show that the low latency achievable in our design allows for the exclusion of delay compensation common in other implementations and that automated reduction of numerical precision can allow the controller design to be compacted. / Thesis / Master of Applied Science (MASc)
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