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The effect of kinetin concentrations (10⁻⁶M and 10⁻⁴M) on distribution of free fatty acids and sterols in plant parts and root exudates of 57-day-old peanut plants grown in nutrient solutions under gnotobiotic conditions was studied. Kinetin was applied to the roots in the culture medium five days before harvest. Upon harvesting root exudates were collected and plants divided into leaves, stems, and roots. The extracted lipid fraction was partitioned using silica gel thin-layer chromatography. Quantitation and identification of free fatty acids and sterols were accomplished by isothermal gas-liquid chromatography. On the basis of ug fatty acid/mg tissue and ug fatty acid/mg lipid kinetin caused no significant changes in total fatty acid concentration. The only significant increase in total sterol concentration was observed in the 10⁻⁴M treatment (ug sterol/mg lipid) in the stems. Use of individual component concentrations, relative percentages, and ratios helped distinguish trends. Shifts in quantity and quality of the free fatty acids and sterols may have been a result of one or a combination of effects attributed to kinetin. Decrease in root exudation in the 10⁻⁶M treatment may have been due to altered membrane integrity. Increase in the sitosterol/ stigmasterol ratio in stems of treated plants may have been a result of delayed senescence. Trends showed transport of free fatty acids and sterols to the roots in 10⁻⁶M treated plants. Kinetin may have effected sterol concentration through changes in HMGCoA reductase activity by unsaturated fatty acids. / Master of Science

The effect of dietary fats on reproductive performance, egg quality, fatty acid composition of tissues and egg yolk, and prostaglandin levels in embryonic tissues in Japanese quail (Coturnix, coturnix japonica) were evaluated. The addition of 5.0% chicken fat (CHX), hydrogenated soybean oil (HSBO), menhaden fish oil (MENH) or soybean oil (SBO) to the maternal and paternal diet altered tissue and yolk composition of the hens and tissue composition of the males to reflect the dietary source. Comparisons were made to chicken fat which served as a control. Feeding MENH increased the omega three fatty acid concentrations in liver, heart, testicle, and yolk. Feeding HSBO and SBO increased the omega six fatty acid concentrations in yolk. Feeding MENH and SBO increased the total monosaturated fatty acids in yolk. The concentrations of polyunsaturated fatty acids were increased by feeding MENH and SBO. Feeding MENH decreased egg production. Feeding MENH and HSBO decreased hatchability. Feeding MENH decreased specific gravity of eggs at day 30, 60, and 90. Feeding CHX increased specific gravity at day 30, 60 and 120. In addition, feeding CHX increased chick weight. Feeding SBO decreased early embryonic death. There were no consistent differences noted in tissue prostaglandin levels of embryos from hens on the differing diets. / Master of Science

The present study sought for practical ways to improve the frying performance of oils without compromising the availability of the essential fatty acids and nutraceuticals. To this end, the influence of temperature, oxygen concentrations, and compositions of minor components on frying performance was investigated. A novel frying protocol, utilizing carbon dioxide blanketing, was developed and found to significantly improve the performance of the frying oil. Optimizing both the amounts and the compositions of endogenous minor components also improved the performance of the frying oil. Twenty one novel antioxidants were synthesized and evaluated under frying and storage conditions. Antioxidant formulations consisting of a combination of endogenous and synthesized antioxidants were developed and tested in an institutional frying operation. A rapid and effective frying test was developed to assess the frying performance of oils and applied antioxidants. Furthermore, a novel procedure for direct hydroxynonenal analysis in frying oil was developed. / xx, 249 leaves; 29 cm

Orientador: Marilisa Berti de Azevedo Barros / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-26T19:46:46Z (GMT). No. of bitstreams: 1 Barbosa_LhaisdePaula_M.pdf: 791897 bytes, checksum: 09592e243e99dc95526c900c976e262a (MD5) Previous issue date: 2015 / Resumo: O consumo de gorduras é essencial na alimentação humana. Os lipídios contribuem ativamente como precursores na síntese de hormônios, fazem parte da estrutura das membranas celulares, compõe a bile, participam da resposta autoimune e do transporte de vitaminas lipossolúveis e garantem o aporte energético da dieta. Contudo, o excesso na ingestão deste nutriente representa risco para o desenvolvimento de uma série de doenças crônicas e o aumento da ingestão de gorduras na dieta das populações foi uma característica marcante no processo de transição nutricional. O objetivo desta dissertação foi analisar o perfil de ingestão de gordura segundo escolaridade e sexo na população adulta residente no município de Campinas. Trata-se de estudo transversal de base populacional que avaliou a dieta de 949 adultos residentes na área urbana do município de Campinas por meio do instrumento Recordatório de 24 horas (R24h). A composição nutricional da dieta foi calculada a partir do software NDS-R versão 2007 e para a análise dos dados foram calculadas as proporções e estimadas as médias com seus respectivos intervalos de confiança de 95% utilizando os modelos lineares generalizados, com base na distribuição do tipo Gamma, para variáveis assimétricas. Como resultados, pode-se observar que a dieta apresentou-se normoglicídica, normolipídica e normoproteica, segundo as recomendações do IOM de 2005, tanto para homens quanto para mulheres e em todos os estratos de escolaridade. Sobre o consumo dos diferentes tipos de gordura, quando analisados por sexo e escolaridade, foi verificado para o sexo masculino maior consumo de gordura total, gordura saturada, gordura monoinsaturada e gordura trans entre os mais escolarizados. Entre as mulheres, foi observado maior consumo de gordura saturada e menor consumo de gorduras poliinsaturadas nos estratos de maior escolaridade. Os resultados do estudo mostraram que, em linhas gerais, o consumo de determinados tipos de gordura é elevado na população estudada e que as gorduras mais consumidas foram àquelas com potencial de prejudicar a saúde: gordura saturada e gordura trans. Pode-se dizer diante dos resultados encontrados que o nível de escolaridade está associado à maior ingestão de gorduras na alimentação já que os indivíduos mais escolarizados, de um modo geral, apresentaram maior consumo médio de gorduras. Estes resultados podem sinalizar uma etapa do processo de transição nutricional em que as populações mais favorecidas apresentam piores indicadores para doenças crônicas não transmissíveis. Por esta razão faz-se necessária a realização de inquéritos alimentares periódicos a fim de monitorar a tendência deste comportamento na população / Abstract: The intake of fat is essential in human nutrition. Lipids actively contribute as precursors in the synthesis of hormones; they are part of the structure of cell membranes and bile, participate in autoimmune response and in the transport of fat-soluble vitamins and ensure the energy intake of the diet. However, the excess intake of this nutrient represents a risk for the development of several chronic diseases and the increased intake of fats in the population diet was a remarkable feature in the nutritional transition process. The aim of this dissertation was to analyse the fat intake profile according to the education and sex in the adult population living in the city of Campinas. This is a cross-sectional population-based study which evaluate the diet of 949 adults living in the urban area of Campinas through the instrument 24-hours Recall (R24h). The nutritional composition of the diet was calculated with the NDS-R software version 2007 and for the data analysis was calculated the proportions and estimated the means with its respective confidence interval of 95% using the generalized linear models, based in the distribution of Gamma type, for the asymmetric variables. As results, it could be observed the diet was normal for carbohydrates, fats and proteins, according to the 2005 IOM recommendations, for both men and women and in all educational strata. For the intake of different types of fat, when analysed by sex and education, it was verified for male a greater intake of total fat, saturated fat, monounsaturated fat and trans fat among the more educated. Among women, it was observed a greater intake of saturated fat and lower intake of polyunsaturated fat in the more educated strata. The results of the study show that, in general, the intake of certain types of fat is high in the population evaluated and the most consumed fats were those with potential to jeopardize health: saturated and trans fat. Thus, the results affirm that education is associated with a greater intake of fat since the most educated, in general, had a greater mean intake of fat. This results can signalize a stage in the process of nutritional transition where the most rich population have the worst indicators for noncommunicable chronic diseases. Therefore it is necessary to perform regular food surveys to monitor this behavior trend in the population / Mestrado / Epidemiologia / Mestra em Saúde Coletiva

Call number: LD2668 .T4 1978 A44 / Master of Science

Call number: LD2668 .T4 1985 C64 / Master of Science

Master of Science / Department of Grain Science and Industry / Jon M. Faubion / Charles E. Walker / Shortening is a major ingredient used in high-ratio layer cakes. Plastic shortenings are most commonly used by the U.S. baking industry, but high levels of trans- or saturated fats cause health concerns. Compared to plastic shortenings, liquid shortenings could significantly reduce the dependence on high melting point fats and the emulsifiers used would enhance the shortening’s functionality. The objective of this research was to compare the influence of different types of fats on the texture and shelf-life of high-ratio layer cakes. Cakes were baked with soybean oil to evaluate the function of three emulsifiers (PGMS, GMS, and Lecithin) on layer cake quality, including volume, cake score, interior visual texture (C-Cell), and firmness (Voland-Stevens). An optimum emulsifier combination was chosen (PGMS 1.8%, GMS 1.0% and Lecithin 0.8%) for addition to the liquid oil. Four groups of layer cakes were baked using: plastic shortening, liquid shortening, liquid oil, or liquid oil plus emulsifier combination. Cake performance and firming over-time were evaluated. The liquid shortening provided the best fresh cake characteristics and cake firmness performance. Liquid oil combined with a combination of added emulsifiers performed very similar in terms of firmness, as did the liquid shortening. This indicated that emulsifiers played an important role on the improvement of cake firmness shelf-life.

Master of Science / Food Science Institute - Animal Science & Industry / Kelly J. K. Getty / Fats and oils are an important topic in the margarine and spreads industry. The selection of these ingredients can be based on many factors including flavor, functionality, cost, and health aspects. In general, fat is an important component of a healthy diet. Fat or oil provides nine calories per gram of energy, transports essential vitamins, and is necessary in cellular structure. Major shifts in consumption of fats and oils through history have been driven by consumer demand. An example is the decline in animal fat consumption due to consumers’ concern over saturated fats. Also, consumers’ concern over the obesity epidemic and coronary heart disease has driven demand for new, lower calorie, nutrient-rich spreads products. Fats and oils can be separated into many different subgroups. “Fats” generally refer to lipids that are solid at room temperature while “oils” refer to those that are liquid. Fatty acids can be either saturated or unsaturated. If they are unsaturated, they can be either mono-, di-, or poly-unsaturated. Also, unsaturated bonds can be in the cis or trans conformation. A triglyceride, which is three fatty acids esterified to a glycerol backbone, can have any combination of saturated and unsaturated fatty acids. Triglycerides are the primary components of animal and vegetable fats and oils. The ratio of saturated to unsaturated fatty acids in these fats and oils has a great impact on their functionality. Common fats and oils include butterfat, beef tallow, fish oils, soybean oil, rapeseed (canola) oil, corn oil, cottonseed oil, olive oil, sunflower oil, palm oil, palm kernel oil, coconut oil, linseed (flax) oil, and safflower oil. Typical fat and oil modification techniques include hydrogenation, interesterification, alternative hydrogenation, fractionation, blending, farm/field practices and genetic modification, and the use of fat replacers. There are many processing techniques that can be utilized in margarine and spreads production. The process can be optimized for each margarine or spreads product. There are many fats and oils in margarine and spreads formulations. Familiarity with commonly used fats and oils in regards to availability, cost, nutrition, chemistry, and functionality are important when creating a cost-effective, functional margarine or spreads product.

Submitted in fulfilment for the requirements for the degree of Doctor of Technology: Biotechnology, Durban University of Technology, 2010. / South African edible oil processing plants produce approximately 3 x 105 tonnes of oil annually with up to 3 tonnes of water for every tonne of oil produced. Wastewater that contains oil extracts varies in organic loading from 30,000 to 60,000 mg.l-1 COD. This wastewater can be used to grow oleophilic fungi to produce valuable industrial products. The global vitamin B market is approximately R25.5 billion with 4500 metric tonnes being produced. A large proportion of this is produced using the fungus Eremothecium gossypii using oil substrates. The aim of this study was to to develop a novel method to produce riboflavin with the aid of fungi, using edible oil effluent (EOE) as substrate, and to optimize the production thereof by statistical experimental design. Four fungi were surveyed for their growth potential on EOE and two, E. gossypii (CBS109.51) and C. famata (ATCC 208.50) were found to produce sufficient riboflavin for further study. Mutation of these organisms using ethylmethane sulphonate (EMS) increased riboflavin production from 3.52 mg.l-1 to 38.98 mg.l-1, an 11-fold increase. An enzyme pathway responsible for this was found to involve isocitrate lyase and comparison of this enzyme’s activity in the mutant against the wild-type using Michaelis-Menten kinetics showed a higher reaction velocity (Vmax) with a reduced substrate affinity (Km) indicating that the mutation was associated with this enzyme. Biomass comparisons were fitted to the sigmoid Gompertz model which was used to compare the wild-type to the mutant and increased specific growth rates and doubling times were observed in mutated cultures of E. gossypi. A strategy of statistical experimental design was pursued to optimize media components and iterative fractional factorial experiments culminating in a central composite optimization experiment were conducted. Statistically verified mathematical models were developed at each stage to identify important media components, predict media interactions, show directions for improvement and finally, predict maximum riboflavin production. An eight-factor resolution IV fractional factorial increased riboflavin production to 112 mg.l-1 followed by a four-factor resolution V experimental design which increased riboflavin production to 123 mg.l-1. A two-factor (yeast extract and NaCl) central composite experimental design predicted a maximum riboflavin production of 136 mg.l-1 which was a 3.5-fold increase from the mutant, and 38.6-fold higher than the E. gossypii wild-type. The optimized value was achieved within predicted confidence intervals in confirmatory experiments. Cost implications for production of riboflavin on EOE were calculated and a 10% technology uptake by the edible oil industry could yield a riboflavin industry with a 63.65 million rand turnover and a potential 24.96 million rand gross profit margin. / National Research Fund.