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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
141

Natural Fibers and Fiberglass: A Technical and Economic Comparison

Zsiros, Justin Andrew 11 June 2010 (has links) (PDF)
Natural fibers have received attention in recent years because of their minimal environmental impact, reasonably good properties, and low cost. There is a wide variety of natural fibers suitable for composite applications, the most common of which is flax. Flax has advantages in tensile strength, light weight, and low cost over other natural fibers. As with other natural and synthetic fibers, flax is used to reinforce both thermoset and thermoplastic matrices. When flax is used in thermoplastic matrices, polypropylene and polyethylene are the main resins used. Although at first glance flax may seem to be a cheaper alternative to fiberglass, this may not necessarily be as advantageous as one would hope. A full economic valuation should be based on raw material costs and full processing costs. Although flax fibers used in composites are generally a waste product from linen flax, they require additional processing which can significantly reduce flax's economic advantage over glass. This paper attempts to place some measure of economic comparison coupled with property comparisons between natural (mainly flax) fibers and glass fibers. Our tests compare tensile, flexural, and drop impact properties, as well as heat sensitivity, and colorant acceptance.
142

Identification of Genomic Regions Involved in Stress Responsiveness in Flax by Genetic Mapping

Bickel, Cory Lyn 20 April 2011 (has links)
No description available.
143

Novel Genomic Remodeling Events In Response to Environmental Stress:Clues from Transgenic Arabidopsis and Flax

BASTAKI, NASMAH K. 03 June 2015 (has links)
No description available.
144

Development of novel bio-derived polymer composites reinforced with natural fibres and mineral fillers

Shakoor, Abdul January 2013 (has links)
Biocomposites exhibit properties like many petrochemical-based polymers composites. They have the potentials be used in the automotive and decking industries and as biodegradable packaging. However, the high cost as well as, poor mechanical and thermal properties have restricted their widespread use. There are a number of technical issues that need to be addressed before bio-composites can be widely used. In this research Polylactic acid (PLA) composites, reinforced with natural fibres (wood, flax) and mineral fillers (talc) were investigated. The thermal and mechanical properties of the composites were studied by means of Differential Scanning Calorimetry (DSC), Tensile Testing and Dynamic Mechanical Analysis (DMA), while morphology and crystallization processes of the composites were studied by hot stage optical microscopy. The experimental results are also compared with different theoretical models of the response of the composites. PLA / wood composites were developed by mixing PLA with wood in different ratios using a melt compounding process. PLA/wood (90/10. 80/20, 60/40), PLA/wood/copolymers (85/10/05, 80/10/10, 75/20/05, 70/20/10, 55/40/05, 50/40/10) and PLA/wood/coupling agent (80/20/silane coating) were the three different composite systems that were developed. Adding increasing amount of wood into the PLA, the thermal properties remain unchanged but the mechanical properties increased significantly, bringing a stiffening effect to the composites. Tensile modulus increased from 4.1± 0.6 to 9.8 ± 1.2 (GPa) as the wood content increased from 0 to 40 (wt %), but the tensile strength at break reduced from 43.8 ± 3.1 to 31.8 ± 2.8 MPa. The experimental results of the PLA-wood composites were modelled according to the Halpin-Tsai equation. The addition of copolymer affected the thermal properties considerably by decreasing the glass transition temperature of the composite. The glass transition temperature dropped from 54 ± 0.7 (0C) to 48 ± 0.36 (0C) when the content of copolymer was increased from 0 to 10 (wt %). The cold crystallization temperature also decreased from 127 ± 1.41 (0C) to 103 ± 2.58 (0C) when the copolymer was incorporated into the PLA/wood composites. The significant aspect was the occurrence of a double peak in the melting endotherm. The degree of crystallinity also increased from 2 ± 0.83 (%) to 11 ± 1.23 (%) when the amount of copolymer was increased to 10 (wt %). PLA, flax and expoidizied natural rubber (ENR) composites were also developed using a melt compounding process. The mechanical properties were affected significantly when the flax fibres were mixed with PLA in the ratios of 10, 20 and 30 (wt %). Addition of flax fibres increased the elastic modulus significantly but reduced the tensile strength and strain at break. To improve the toughness of the PLA- Flax composites, ENR was incorporated into the PLA- Flax composites. In order to balance the modulus of the reinforcement and the matrix, the PLA- Flax and ENR composites were annealed above the glass transition temperature and the degree of crystallinity increased from 2 to 35 (%). The integral blending of PLA, Flax and ENR did not affect the brittle fracture but introducing a masterbatch of flax fibres and ENR into the PLA matrix during melt processing had a considerable effect on the fracture behaviour of the composites. The elastic modulus of the composites decreased due to the elastomeric content in the composites and there was an increase in elongation-to-break. The effect of talc on the crystallinity and mechanical properties of a series of polylactic acid (PLA) / talc composites was investigated. PLA talc composites were developed by incorporating different types of the talc into the PLA in the ratios of 10, 20 and 30 (wt %). The composites were prepared by melt blending followed by compression moulding. It was found that talc acted as a nucleating agent and increased the crystallinity of the PLA from 2% to 25%. There was significant improvement in Young s modulus of the composites with increasing talc addition and these results were found to fit the Halpin Tsai model. Thermo-mechanical tests confirmed that the combination of increased crystallinity and storage modulus leads to improvement in the heat distortion properties.
145

Natūralaus pluošto įtaka polimerinių kompozitų savybėms / Natural fibre composites on the mechanical properties of polymer

Žymantas, Andrius 16 June 2014 (has links)
Tyrimo tikslas – įvertinti skirtingo natūralaus pluošto įtaką kompozicinės medžiagos polietilenas – pluoštas“ mechaninėms savybėms. Bandinių gamyba bei medžiagų mechaninių savybių tyrimai atlikti 2013/2014 m. Aleksandro Stulginskio universitete, Jėgos ir transporto mašinų inžinerijos instituto ir Žemės ūkio inžinerijos ir saugos instituto laboratorijose. Tyrinėjami kompozitai armuoti trimis skirtingai natūraliais pluoštais: kanapių, dilgėlių ir linų. Polietileno klijų kompozitas armuojamas keturiais kiekiais pluošto: 5 %,7 %, 10 % ir12 %. Tempimo ir lenkimo bandymai atlikti „Instron“ medžiagų savybių tyrimo stendu, nustatyta apkrova, kurią atlaiko bandinys ir deformacija. Atlikus lenkimo ir tempimo tyrimus nustatyta, kad geriausia armuojanti medžiaga yra kanapių pluoštas. Tempiant atlaikė didžiausią apkrovą – 753,7 N ir deformavosi 4 mm. Lenkiant kanapių pluoštu armuotas bandinys atlaikė 24,3 N apkrovą ir deformavosi 11,6 mm. / The aim - to evaluate the influence of different natural fiber composite polyethylene - fibers' mechanical properties. Specimen fabrication and mechanical properties of the materials were tested in 2013/2014 year. Alexander Stulginskio University, Power and Transport Machine Engineering and the Institute of Agricultural Engineering and Safety Institute laboratories. Reinforced composites are studied in three different natural fibers: hemp, ramie and flax. Polyethylene adhesive composite reinforced with four volumes of fiber: 5%, 7%, 10%, and 12%. Tensile and flexural tests were examined by using "Instron" material properties testing machine down load the sample and can withstand deformation. After bending and tensile tests showed that the best reinforcement material is hemp. Tensile withstood the maximum load - 753.7 N and deformed 4 mm. bending of hemp fiber reinforced sample withstood 24.3 N load and 11.6 mm.
146

Les toiles d'Armentières. Entreprises et entrepreneurs du lin (XIXe-XXIe siècles) / The city of linen ». Entrepreneurs and company of Armentières (Nineteenth-twenty-first century)

Smaghue, Nicolas 25 September 2015 (has links)
Longtemps appelée la « cité de la toile », Armentières a vu sa prospérité reposer, un siècle durant, sur la fabrication et la commercialisation des tissus majoritairement en lin. Pourtant, si cette ville manufacturière a largement contribué à la renommée textile de la région lilloise, elle est restée en retrait par rapport à Lille, Roubaix et Tourcoing. C’est si vrai qu’aujourd’hui encore, on peine à retrouver les traces du riche patrimoine industriel dont elle disposait il y a trois ou quatre décennies. Contrairement à ses prestigieuses voisines, faute de réhabilitation et de reconversion de son bâti usinier, elle a tourné le dos à son passé comme si celui-ci ne valait pas la peine que l’on s’y attarde. Aussi, entre requalification des friches et absence de volonté pour valoriser ce qui reste du patrimoine industriel, on oublierait presque qu’Armentières a été de temps immémoriaux un centre manufacturier non pas secondaire mais second. Grâce aux nouvelles techniques numériques et aux ressources désormais mises à la disposition du chercheur, il est malgré tout possible de reconstituer l’empreinte que l’industrie a laissée dans le tissu urbain. Emprise foncière des bâtiments dévolus à la fabrication ou à la commercialisation des toiles, procédés constructifs et matériaux, organisation des lieux productifs : il a fallu exhumer et agencer toute la documentation disponible pour faire ressurgir un passé en quasi-totalité disparu. Pourtant, quand il s’est agi, dès le milieu du XIXe siècle, de répondre aux défis des marchés, les entrepreneurs locaux ont réussi à développer la convoitise de la clientèle pour le linge de maison et de table. Ils ont également répondu avec succès aux demandes toujours plus exigeantes de l’armée et des administrations. Bref, en valorisant la tradition tout en répondant aux exigences de produits « techniques », les industriels d’Armentières sont parvenus, autour du travail du lin, à imposer la localité comme une véritable marque de fabrique pour mieux soutenir la comparaison avec leurs puissants voisins. Mais cette conquête des marchés n’aurait pas eu lieu sans une indéniable capacité à maîtriser le travail d’une fibre longtemps rétive à la mécanisation. Aussi, pénétrer dans les usines, recenser les matériels et retrouver les gestes du fileur et du tisseur étaient indispensable pour comprendre comment, par delà les vicissitudes de la conjoncture, les défis techniques ont été relevés afin de conjuguer production de masse, qualité de la marchandise et coûts de fabrication. Histoire des lieux de la production, des marchandises et de la fabrication des toiles, ce travail entend également revenir sur le milieu des patrons armentiérois déjà étudié, non sans complaisance envers son milieu d’origine, par Lambert-Dansette. Les sources de l’Enregistrement et les actes notariés, entre autres, ont permis de revenir sur les origines géographiques et sociales des entrepreneurs puis de s’interroger sur les montages juridiques, les financements et les modes de gestion qui ont rendu possible cette aventure industrielle. C’était là l’occasion de mesurer la spécificité du monde des liniers au sein du patronat régional et, plus encore, de comprendre leur étonnante capacité à repousser, à partir des années 1930, les échéances d’une mort annoncée. Soucieux de souscrire à la « main invisible du marché » et de sanctifier tous les dogmes libéraux, les patrons d’Armentières ne s’arrangent-ils pour bénéficier de l’aide de l’État dont ils attendent à la fois une relative souplesse à leur égard et, singulièrement une protection infaillible ? En définitive, le choix délibéré de faire l’histoire en longue durée des entreprises et des entrepreneurs du lin à une échelle locale permet de réinterroger les modes de coordinations et les combinaisons multiples qui, en mariant le local et le global, autorisent à saisir d’un seul tenant tous les déterminants spatiaux du capitalisme. / Armentières was called “the city of linen” for a long time, and it prospered for a century thanks to the manufacture and trade of fabric, mainly linen. But even if this manufacturing town greatly contributed to making the Lille area famous for its textiles, it always remained in the background compared to Lille, Roubaix and Tourcoing. This is so true that today it is still difficult to find memories of the rich industrial heritage that Armentières had three or four decades ago. Unlike its prestigious neighbours, Armentières turned its back on its past as if it was not worth any attention, and this was reflected notably in a lack of renovation and renewal of its industrial buildings. Entire industrial wastelands are being reshaped and no one seems willing to make what is left of the town’s industrial heritage more attractive, which could make us forget that since ancient times Armentières was a major manufacturing centre. However, thanks to the new digital technologies and the resources that are available available to researchers, it is now possible to recreate the imprint that the industry has left on the urban network. Whether it was about the impact on property of buildings devoted to fabric manufacturing and trade, about building processes and materials, or about the organization of manufacturing facilities, it was necessary to find and organize the whole range of documents available in order to bring back to life a past that had almost completely disappeared. Local entrepreneurs managed to stimulate customer desire for household and table linen when they had to face the challenges of the market in the middle of the 19th Century. They also responded successfully to the increasingly demanding requests from the army and administration. Putting tradition forward while also providing technically demanding products, the manufacturers from Armentières focused on linen and managed to turn the area into a label that guaranteed authenticity and quality, ensuring that Armentières was fully able to compete with its powerful neighbours. But they would never have conquered the market if they had not mastered the ability to work with a type of fabric that was hard to manufacture industrially. Thus, taking a close look at the factories, listing the machines and tools that were used, and studying precisely how the spinner and the weaver worked were necessary steps to face up tothe the technical challenges in a difficult economic context, so as to handle mass production, product quality and manufacturing costs at the same time. This dissertation proposes to study the history of the manufacturing facilities, the manufacture of goods and fabrics but also to deal with the social class of Armentières’ factory owners, which was already studied by Lambert-Dansette, not without some self-indulgence towards the social class he came from. Various sources have allowed me to take a close look at the geographical and social origins of these entrepreneurs and then to look into the various legal, financial and management condidtions which made this industrial adventure possible. This allowed me to confront the experience of the linen makers with that of the other industrialists in the region, and to understand their amazing ability to push back and delay the inevitable death of their industry from the 1930s onwards. Willing to obey “the invisible hand of the market” and to follow all liberal theories wholeheartedly, can we not say that the industrialists of Armentières did everything they could to benefit from state assistance? Not only did they expect to receive state support, they also sought to have their sector benefit from considerable state protection.
147

Modification of flax fibres for the development of epoxy-based biocomposites : Role of cell wall components and surface treatments on the microstructure and mechanical properties / Modification de fibres de lin pour le développement de bio-composites à matrice époxy : Rôle des composants des parois cellulaires et des traitements de surface sur la microstructure et les propriétés mécaniques

Acera Fernandez, José 15 December 2015 (has links)
Les fibres végétales peuvent être considérées comme une alternative intéressante aux fibres de verre pour la fabrication de matériaux composites. En effet, elles présentent des caractéristiques physiques intéressantes, telles que leur faible densité et leurs bonnes propriétés mécaniques spécifiques, qui peuvent rivaliser avec les composites renforcés de fibres de verre. En outre, les fibres végétales sont obtenues à partir de ressources renouvelables, et présentent généralement moins d'impacts environnementaux lors de leurs phases de production, d’utilisation et en fin de vie. Contrairement aux fibres de verre, les fibres végétales, telles que des fibres de lin, présentent des structures hiérarchiques complexes composées essentiellement de cellulose, hémicellulose, lignine, ciments peptiques et extractibles lipophiliques (cires, acides gras, etc.). Cette composition varie selon les espèces, le lieu et les conditions de croissance, la maturité de la plante, etc. De la même façon, la composition biochimique et la structure des produits et des sous-produits issus du lin sont soumis à de grandes variations selon les étapes successives de transformation réalisées à partir des tiges de lin jusqu’aux fils et tissus. Cela influence fortement les propriétés finales des fibres de lin et de leurs biocomposites. La première partie de cette étude se concentre sur la caractérisation de fibres de lin au cours de leurs étapes successives de transformation. Une homogénéisation de la composition chimique est observée dans les étapes finales de transformation, ainsi qu’une augmentation des propriétés en traction longitudinale des mèches de fibres de lin. La deuxième partie traite de l'utilisation de différents traitements de lavage appliqués sur des tissus d’étoupes de lin et leur influence sur l'extraction des composants de la paroi cellulaire des fibres, ainsi que sur la microstructure et les propriétés mécaniques de biocomposites époxy/lin. Il est montré que les composants de la paroi cellulaire jouent un rôle clé dans la dispersion des mèches et des fibres élémentaires de lin et sur le comportement mécanique transversal de leurs biocomposites. Enfin, l'application de différents traitements de fonctionnalisation sur des tissus de fibre de lin est étudiée afin d'améliorer l'adhérence interfaciale entre les fibres et la matrice. L'utilisation de molécules de type organosilanes (aminosilane, époxysilanes) et de molécules biosourcés (acides aminés et polysaccharides) est étudiée. Une augmentation de la rigidité en traction longitudinale et de la rigidité et de la contrainte maximale en traction transverse est observée en raison de l'amélioration de l'adhésion interfaciale par la fonctionnalisation de surface des fibres avec des molécules d'origine biosourcé et non-biosourcé. / Natural fibres can be considered as a relevant alternative to glass fibres in the manufacture of composite materials. Indeed, they present interesting physical characteristics, such as low density and good specific mechanical properties, which can compete with glass fibre reinforced composites. Moreover, natural fibres are obtained from renewable resources, and generally present lower environmental impacts during their production and use phases and their end of life. Unlike glass fibres, natural fibres, such as flax fibres, are complex hierarchical materials composed essentially of cellulose, hemicellulose, lignin, peptics cements and lipophilic extractives (waxes, fatty acids, etc.). This composition varies among species, collection site, plant maturity, batches, etc. Besides, the biochemical composition and structure of flax products and sub-products undergo wide variations according to the transformation steps from stems to yarns and fabrics. This influences greatly the final properties of flax fibres and their biocomposites. The first part of this study is focused on the characterization of flax fibres during their successive transformation steps. A homogenization of the chemical composition is observed at the final transformation steps, as well as an increment of the longitudinal tensile properties of flax yarns. The second part deals with the use of different washing treatments applied on flax tow fabrics and their influence on the extraction of flax cell wall components and the resulting microstructure and mechanical properties of epoxy/flax fibres reinforced biocomposites. It is shown that cell wall components play a key role in the flax yarns and elementary fibres dispersion and transverse mechanical behaviour of biocomposites. Finally, the application of different functionalization treatments onto flax fibres fabrics is investigated in order to improve the interfacial adhesion between fibres and matrix. The use of non-bio-based organosilane molecules (aminosilane, epoxysilane) and bio-based molecules (amino-acids and polysaccharides) is studied. Improvedstiffness in longitudinal tension test and stiffness and tensile strength in transverse tension test are observed due to the improvement of interfacial adhesion by surface functionalization of the fibres with both bio-based and non-bio-based molecules.
148

Compréhension du métabolisme central et lipidique chez les plantes et les levures oléagineuses : approche fluxomique / Understanding of central and lipid metabolism in oleaginous plants and yeasts : fluxomic approach

Degournay, Anthony 19 October 2018 (has links)
Une population mondiale croissante et l’épuisement des ressources fossiles a conduit à une augmentation de la demande alimentaire et énergétique. Si les plantes oléagineuses sont majoritairement exploitées pour leurs fruits et leurs graines riches en huiles dans le secteur agroalimentaire, elles sont également valorisées comme alternative aux produits pétrosourcés (biolubrifiants, biocarburants). La production de lipides et d’acides gras inhabituels a rapidement suscité un intérêt envers les organismes unicellulaires : les levures. L’objectif de ce travail consiste à étudier deux modèles biologiques : la graine de lin (Linum usitatissimum), dont l’huile est constituée à 57% d’oméga-3, et la levure oléagineuse Yarrowia lipolytica, exploitée comme châssis biotechnologique. L’approche utilisée pour appréhender le métabolisme lipidique est la fluxomique. De plus, la conception d’un modèle prédictif reposant sur un marquage isotopique (MFA) ou la contrainte (FBA) permet une analyse dynamique du métabolisme. L’étude comparative de trois lignées de lin (teneurs en huile et oméga-3 différentes) a permis une meilleure compréhension des mécanismes menant à l’accumulation des lipides (jusqu’à 44,2 g.100g-1 MS). Ainsi, nous avons pu montrer que l’assimilation du saccharose et la remobilisation de l’amidon sont essentiels à la synthèse des précurseurs et du NADPH nécessaires à la synthèse des AG. Une forte implication de la glycolyse cytosolique et de la voie des pentoses phosphate plastidiale a pu être notée, tandis que la synthèse des protéines et de la paroi cellulaire a été une étape plutôt limitante. De plus, la PDAT semblerait être une enzyme essentielle à l’incorporation d’acides gras polyinsaturés dans les TAG. L’étude de trois souches de Yarrowia lipolytica a également permis d’appréhender le métabolisme de la levure. L’assimilation d’une source de carbone alternative au glucose (glycérol) a entraîné une redirection métabolique majeure vers la néoglucogénèse. Le flux majoritaire pour la synthèse des TAG emprunte la glycolyse et une partie du cycle de Krebs, afin de convertir le citrate en acétyl-CoA. L’optimisation de la voie Kennedy (GPD1 et DGA2) a permis une amélioration du contenu en lipides : +72% par rapport à une souche optimisée pour la synthèse des acides gras inhabituels (expression du gène LRO1, codant pour une PDAT). Les principales voies compétitives sont la synthèse de glucides de réserve et la sécrétion de citrate, réprimée ici grâce à une assimilation de glucose modérée. La PDAT est là encore impliquée dans l’accumulation des acides gras inhabituels. / Growing world population and depletion of fossil resources have led to an increasing food and energy demand. While oleaginous plants are mostly cultivated for their fruits or their seeds in food industry, they are also valued in as an alternative to petrochemicals (biolubricant, biofuels). The production of lipids and unusual fatty acids increased the interest for unicellular organisms: yeasts. The aim of this work is to study two biological models: flax seed (Linum usitatissimum), whose oil is made up of 57% omega-3, and yeast Yarrowia lipolytica, exploited as a biotechnological chassis. The approach used to understand lipid metabolism is fluxomics. In addition, the development of a predictive model based on isotopic labelling (MFA) or constraint-based one (FBA) allows a dynamic analysis of the metabolism. The comparative study of three flax lines (with different oil and omega-3 levels) provided a better understanding of the mechanisms leading to lipid accumulation (up to 44.2 g.100 gDW-1). Therefore, we have been able to show that sucrose assimilation and starch remobilization are essential for fatty acid precursors and cofactors synthesis. Strong involvements of cytosolic glycolysis (G3P, acetyl-CoA) and pentose phosphate pathway (NADPH) have been noted, while protein and cell wall synthesis are limiting steps. In addition, PDAT would be a central enzyme for the incorporation of PUFA into TAGs. The study of three Yarrowia lipolytica strains also helped us to better understand yeast metabolism. The assimilation of an alternative carbone source to glucose, glycerol, led to a major metabolic redirection towards gluconeogenesis. The TAG synthesis flux especially uses glycolysis and a part of TCA cycle to convert citrate into acetyl-CoA. Kennedy pathway optimizations (GPD1 and DGA2 gene overexpression) allowed a lipid content improvement: +72% compared to a strain optimized for the synthesis of unusual fatty acids (LRO1 gene expression, encoding for a PDAT enzyme). The main competitive pathways are carbohydrate synthesis (glycogen) and citrate secretion (here repressed thanks to slow glucose assimilation. PDAT (LRO1 gene) also led to unusual fatty acid accumulation.
149

Potencial quimiopreventivo de lipídios estruturados obtidos por interesterificação da tributirina com o óleo de linhaça na hepatocarcinogênese / Chemopreventive potential of structured lipids obtained by interesterification of tributyrin with flaxseed oil in hepatocarcinogenesis.

Heidor, Renato 11 February 2016 (has links)
O carcinoma hepatocelular (HCC) apresenta mau prognóstico o que torna importante sua quimioprevenção. Nesse sentido, a tributirina (TB), um inibidor de desacetilases de histonas (HDACi), mostrou-se um quimiopreventivo promissor da hepatocarcinogênese. Avaliaram-se aqui efeitos quimiopreventivos de lipídios estruturados (EST) obtidos por interesterificação enzimática a partir da TB com o óleo de linhaça (LIN). Ratos foram tratados com EST (grupo EST; 165 mg/100g peso corpóreo [p.c]), TB (grupo TB; 200 mg/100g p.c), LIN (grupo LIN; 133 mg/100g p.c), mistura de TB com LIN (grupo LIN; 165 mg/100g p.c) ou maltodextrina (MD) (grupo MD; controle isocalórico; 300 mg/100g p.c) diariamente durante 8 semanas consecutivas por gavagem. Duas semanas após início dos tratamentos, os animais foram submetidos ao modelo de hepatocarcinogênese do \"hepatócito resistente\" (RH). Os grupos EST e TB apresentaram atividade quimiopreventiva bloqueadora e supressora, respectivamente, da hepatocarcinogênese. TB induziu a apoptose, ao contrário dos EST. O tratamento com TB resultou na acetilação e trimetilação da H3K9 e H3K27, enquanto EST atuaram somente na trimetilação das mesmas. Quando analisada a expressão de genes envolvidos com modificações em histonas, EST e TB reduziram a expressão de Ezh2 e de Hdac4. Por outro lado, somente os EST aumentaram a expressão de Hdac6. Tal efeito por parte dos EST merece ser mais investigado, uma vez que esta desacetilase vem sendo sugerida como alvo potencial para o desenvolvimento de fármacos. Em conclusão, a atividade quimiopreventiva de EST e da TB envolve na hepatocarcinogênese experimental mecanismos epigenéticos que podem ou não ser distintos. / Hepatocellular carcinoma (HCC) has a poor prognosis, which makes its chemoprevention important. Tributyrin (TB), which is a histone deacetylase inhibitor (HDACi), is a promising chemopreventive agent of hepatocarcinogenesis. The chemopreventive effects of structured lipids (STLs) that were obtained by the enzymatic interesterification of TB with flaxseed oil (FSO) were evaluated in the present study. Rats were treated with STLs (STL group, 165 mg/100 g body weight (bw)), TB (TB group, 200 mg/100 g bw), FSO (FSO group, 133 mg/100 g bw), TB mixed with FSO (BLD group, 165 mg/100g bw) or maltodextrin (MD) (MD group; isocaloric control; 300 mg/100 g bw) daily for eight consecutive weeks by gavage. Two weeks after the initiation of treatment, the animals were subjected to the resistant hepatocyte hepatocarcinogenesis model (RH). The STL and TB groups developed blocker and suppressive chemopreventive activity against hepatocarcinogenesis, respectively. TB treatment induced apoptosis, unlike the STL treatment. Additionally, TB treatment resulted in the acetylation and trimethylation of H3K9 and H3K27, whereas the STLs acted only in the trimethylation of these histones. When analyzing the expression of genes involved in histone modifications, the STLs and TB reduced enhancer of zeste homolog 2 (Ezh2) and histone deacetylase 4 (Hdac4) gene expression. Conversely, only the STLs increased Hdac6 gene expression. This effect of the STLs warrants further investigation because this deacetylase has been suggested as a potential drug development target. In conclusion, the chemopreventive activities of the STLs and TB in experimental hepatocarcinogenesis involve epigenetic mechanisms that may be distinct.
150

Approche de l'impact des matériaux bio-sourcés sur la qualité perçue des produits : cas de la fibre de lin / Approach of bio-sourced materials impact on product's perceived quality : the case of flax fiber

Avramescu, Ana-Maria 13 December 2013 (has links)
Dans un contexte d’éco-conception, les efforts d’innovation amènent de nouveaux matériaux dits bio-sourcés qui participent à réduire l’impact des produits sur l’environnement. S’agissant de nouveaux matériaux, l’innovation porte également sur l’amélioration de la qualité perçue par le consommateur. Le challenge est donc de concevoir des produits respectueux de l’environnement à l’aide de nouveaux matériaux écologiques tout en garantissant une qualité perçue au moins identique à celle des matériaux synthétiques classiques. Le design sensoriel, par l’utilisation de l’évaluation sensorielle, est l’une des disciplines qui permet de prendre en compte le ressenti sensoriel des consommateurs. Cependant, dans la littérature, peu de travaux portent sur l’application des méthodes d’évaluation sensorielle à des matériaux bio-sourcés. Face à ce constat, nos travaux se proposent d’étudier la possibilité d’évaluer, avec des méthodes d’analyse sensorielle, un matériau bio-sourcé à base de fibres de lin de la même manière qu’un synthétique à base de fibres de verre. Plus précisément, nos travaux proposent une approche basée sur une évaluation sensorielle tactile et une évaluation instrumentale objective de la surface de ces deux matériaux. Nos résultats ont permis de valider l’application des méthodes d’évaluation sensorielle tactile sur un matériau bio-sourcé. Nos travaux ont permis la définition de profils sensoriels pour le toucher concernant le matériau à base de fibres de lin. Les résultats obtenus ont aussi permis de valider la possibilité de corréler certains indicateurs tactiles subjectifs avec des mesures instrumentales objectives. L’ensemble des connaissances développées dans le cadre de ces travaux offre aux concepteurs une aide précieuse dans le choix des matériaux bio-sourcés en éco-conception et plus généralement en conception de produits. / In the eco-design context, innovation efforts come with new bio-sourced materials that are meant to reduce products’ environmental impact. Insofar as these products are made with new materials, the innovation is also concerned about their perceived quality by consumers. More precisely, the challenge is to reach a compromise between environmental and marketing issues. Indeed, to be satisfying, the perceived quality of the environmental-friendly products has to be similar to the one of products made with classical synthetic materials. Sensorial design, through sensorial evaluation, is the academic discipline that takes into account the sensorial consumers’ perception. However, very few previous studies were led concerning the application of sensorial evaluation methods to bio-sourced materials. The present work proposes to make up for this lack of knowledge. More precisely, we evaluate both a flax fiber bio-sourced material and a glass fiber synthetic material using the same sensorial analysis methods. This evaluation combines a subjective sensorial tactile evaluation to an objective instrumental evaluation of both these materials’ surfaces. The results validated the application of tactile sensorial evaluation methods to a bio-sourced material. Moreover, tactile sensorial profiles were defined for flax fiber bio-sourced materials. Results also enabled to correlate subjective tactile measures with tactile objective instrumental measures. The present gathered knowledge offer, to designers concerned with eco-design and more generally by product design process, a new valuable bio-sourced material decision making aid.

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