<p>Instability in the administration of municipalities is a particularly serious problem in the Western Cape because of its large number of coalition councils. Coalitions have led to<br /> frequent changes in local governance and to constant changes in political and administrative leadership. Due to the fluidity in local government, the politics of the day have become synonymous with back-stabbing, secret agreements and arrangements between politicians and political parties wishing to align themselves in such a way as to gain control of the councils.1 A municipality is required by the Constitution2 to structure and manage its administration and budgets, budgeting and planning processes so as to give priority to the basic needs of the community and to promote the social and economic development of the community.3 Legislation further prescribes that &ldquo / a municipality must within its administrative and financial capacity establish and organize its administration in a manner that would enable the municipality to establish clear relationships, facilitate coordination, cooperation and communication between (i) its political structures and political office bearers and its administration / (ii) its political structures, political office bearers and administration and the local community&rdquo / .4 It may therefore be argued that a municipality subscribing to the abovementioned prescripts should be functioning effectively. However, in practice continuous administrative and political instability adversely impacts on a municipality‟s capacity to provide service delivery to the community. The 2006 local government elections resulted in only four out of 30 municipalities in the Western Cape having a single party with more than 50% of the seats (outright majority). The remaining 26 municipalities were governed by coalitions of two or more parties. In 2001, Parliament introduced floor-crossing legislation which allowed Members of Parliament, Members of Provincial Legislatures and local government councillors to change their political party (or form a new party) and retains their seats when they did so.5 As a result of the 2007 floor-crossing legislation, the number of municipalities with an outright majority increased to 7. Power changes continued to occur even after the 2007 floor-crossing as a result of by-election outcomes or new internal coalition arrangements. Coalition government in the Western Cape remains a reality following the 18 May 2011 local government elections: the Democratic Alliance won 12 municipalities outright, the African National Congress won 1, and in 12 municipalities there was no outright winner. Of the 12 last-mentioned municipalities, 7 municipalities produced hung municipalities, i.e. Bitou, Witzenberg, Laingsburg, Hessequa, Theewaterskloof, Matzikama and Prince Albert.</p>

Made available in DSpace on 2015-03-26T13:44:21Z (GMT). No. of bitstreams: 1 texto completo.pdf: 645287 bytes, checksum: 08e09df8d79cf20b4b597198af6c3f95 (MD5) Previous issue date: 2011-02-15 / Salman Rushdie, currently one of the great names of contemporary literature in English is recognized for his contribution on representations in which the concept of identity, local and global intertwine to language as an ideological construct. Literature is thus a proper space for the questioning gaze on the inherent authoritarianism of the former. However, rather than a speech explicitly whistleblower or propaganda, the author establishes a fictional game on the paths of History in his books, destabilizing so-called official truths. This way, he prints his autobiographical experience towards censorship. In this work, then we aim at analyzing the constitution of the subject who is undermined by Power in Haroun and the Sea of Stories (1991) from two points: in what it concerns to the fluidity of language and to the inherent voices of the speech. These, on their turn, are linked to the issue of the authorship and the place of formation of this subject, seen inside his contextual complexity which involves other issues, such as gender. / Salman Rushdie, atualmente um dos grandes nomes da literatura contemporânea em língua inglesa, é uma figura reconhecida pela sua contribuição às representações em que o conceito de identidade, local e global se entrelaçam aos da linguagem enquanto uma construção ideológica. A literatura revela-se, assim, um espaço propício ao olhar que questiona o uso autoritário que pode ser feito daquela. Contudo, ao invés de um discurso explicitamente denunciador ou panfletário, o autor estabelece um jogo ficcional em torno dos meandros da História em seus livros, desestabilizando pretensas verdades oficiais. Dessa forma, imprime sua experiência autobiográfica face à censura para incitar a desvalorização das ficções a serviço da opressão. Neste trabalho, analisaremos, portanto, a constituição do sujeito suscetível às mazelas do Poder em Haroun e o Mar de Histórias (1990) a partir de dois pontos: quanto à da fluidez da linguagem e das vozes inerentes do discurso. Estas, por sua vez, se ligam à questão da autoria e à reflexão do espaço de formação desse sujeito, visto dentro de sua complexidade contextual que envolve também outros parâmetros, tais como o gênero.

[pt] A performance de protocolos geralmente empregados na caracterização reológica de pastas de cimento é investigada. Para este fim duas pastas de cimento foram utilizadas, Pasta A e Pasta B, com a mesma densidade e razão água-cimento, diferindo quanto aos aditivos empregados. O procedimento API (American Petroleum Institute), tal qual, sua modificação é estudada. Curvas de escoamento são determinadas com o auxílio de um reômetro rotacional através de três diferentes métodos (ramp up/ramp down, ramp down e viscosidade mínima). O procedimento API gerou curvas de escoamento que superestimaram (Pasta A) ou subestimaram (Pasta B) a viscosidade dentro da faixa de cisalhamento estudada. Os desvios tornam-se particulamente maiores em baixas taxas, e para Pasta B, por causa de efeitos tixotrópicos, o regime permanente não é atingido. A modificação do protocolo API investigada resultou em curvas de escoamento similares ao método de viscosidade mínima. Um novo modelo constitutivo fenomenológico irreversível é proposto para descrição reológica de pastas de cimento (voltadas para poços de petróleo) sob escoamento de cisalhamento simples. Para esse objetivo uma série de experimentos foram realizados, como taxa de deformação constante, construção e destruição da microestrutura. O modelo proposto utiliza a própria fluidez, recíproca da viscosidade, para descrever a evolução da estrutura da pasta. O efeito macroscópico do fenômeno de hidratação na reologia é introduzido por meio de uma função exponencial para tensão limite de escoamento. Todos os dez parâmetros necessários no modelo são experimentalmente obtíveis. Os resultados mostraram boa concordância entre os dados experimentais e a modelagem, embora pequenas diferenças tenham sido observadas para taxas de deformação abaixo de 7,1 s(−1). / [en] The performance of reological protocols usually employed for the rheological characterization of oil well cement slurries is investigated. To this end two cement slurries were employed, namely Paste A and Paste B. The API procedure is addressed and a modification to it is investigated. Flow curves are determined with the aid of a rotational rheometer using three different methods (ramp up/down, ramp down, and minimum-viscosity). The API procedure yielded flow curves that significantly overestimate (Paste A) or underestimate (Paste B) the viscosity throughout the whole range of shear rate. The deviations become particularly dramatic in the low end of this range and for Paste B, clearly because the steady state is not attained due to a thixotropic behavior. The investigated modification to the API procedure resulted in flow curves similar to the ones based on the minimum-viscosity method. A novel irrevesible phenomenological constitutive model is proposed to describe oil well cement slurry rheology under simple shear flow. To this end several rheological experiments were conducted, namely constant shear rate, construction and microstructural destruction experiment. The proposed model employ the fluidity itself, reciprocal of viscosity, to describe the paste s structure evolution. The macroscopical rheological effect of hydration phenomena is introduced in the model by means of a yield stress exponential function. All ten parameters needed in the model are experimentally obtainable. The present results have shown a good agreement between experimental data and modeling, albeit minor differences were observed for low shear rates below 7.1 s(−1).

Pseudomonas aeruginosa é uma proteobactéria do grupo gama muito versátil, capaz de colonizar ambientes variados e infectar hospedeiros filogeneticamente distintos, incluindo humanos imunocomprometidos. Os fatores sigma de função extracitoplasmática (ECF) são membros de sistemas de sinalização de superfície celular (CSS), abundantes em P. aeruginosa. Vinte genes codificando fatores sigma ECF estão presentes nos genomas sequenciados de P. aeruginosa, a maioria fazendo parte de sistemas TonB relacionados à captação de ferro. Neste trabalho, seis fatores sigma pobremente caracterizados foram superexpressos na linhagem PA14 a partir de um promotor induzível por arabinose para investigar seu papel na expressão dos sistemas de dois componentes PvrSR e RcsCB, que atuam na regulação da fímbria CupD, além de sua influência no crescimento de culturas de P. aeruginosa. Não foi observado efeito positivo de nenhum dos fatores sigma testados na expressão dos sistemas de dois componentes e a superexpressão de cinco deles tampouco levou a qualquer alteração no crescimento, porém a produção de piocianina foi alterada na superexpressão de PA14_55550 e a superexpressão de PA14_26600 e PA14_46810 levou a um discreto aumento no início da formação de biofilme em PA14. Por outro lado, culturas superexpressando &#963;x (ALB04) apresentaram um perfil alterado de lipopolissacarídeo e uma curva de crescimento bifásica, alcançando precocemente uma fase estacionária seguida de uma recuperação do crescimento até uma segunda fase estacionária. Durante a primeira fase estacionária, a maior parte das células aumenta de tamanho e morre, mas as células remanescentes retornam à morfologia selvagem e seguem para a segunda fase de crescimento exponencial. Isso não acontece devido a mutações compensatórias, uma vez que células coletadas de pontos tardios da curva e diluídas em meio novo repetem este comportamento. Apesar de trabalhos com a linhagem PAO1 associarem &#963;x à transcrição de oprF, que codifica a principal porina não específica de Pseudomonas, nas condições dos nossos ensaios em PA14 a expressão dessa porina não foi induzida pela superexpressão de &#963;x. Assim, os efeitos observados nessa superexpressão também não podem ser atribuídos a OprF. A transcrição de oprF em PA14 mostrou-se majoritariamente dependente da região promotora a que se atribui a ligação de &#963;70, ao contrário dos relatos na literatura da dependência da região de ligação a &#963;x. Análises proteômicas foram realizadas para investigar os elementos envolvidos nesses efeitos de superexpressão de &#963;x, o que revelou a indução de diversas enzimas envolvidas na via de biossíntese de ácidos graxos. As células superexpressando &#963;x apresentam uma maior proporção de ácidos hexadecanoico (C16) e hexadecenoico (C16:1) e dados de anisotropia mostram uma maior fluidez da(s) membrana(s). Este trabalho é o primeiro relato de um fator sigma ECF envolvido em biossíntese de lipídeos em P. aeruginosa. / Pseudomonas aeruginosa is a very versatile gammaproteobacteria, able to colonize different environments and to infect phylogenetically distinct hosts, including immunocompromised humans. The extracytoplasmic function sigma factors (ECFs) are members of cell signaling systems (CSS), abundant in P. aeruginosa. Twenty genes coding for ECF sigma factors are present in the sequenced genomes of P. aeruginosa, most of them being part of TonB systems related to iron uptake. In this work, six poorly characterized sigma factors were overexpressed in strain PA14 from an arabinose inducible promoter to investigate their role in the expression of the two-component systems PvrSR and RcsCB, which regulates CupD fimbria, and their influence in P. aeruginosa cultures growth. None of the tested sigma factors led to two-component systems upregulation and overexpression of five of them caused no change in the growth profile, but pyocyanin production was altered in PA14_55550 overexpression and PA14_26600 and PA14_46810 overexpression led to a slight increase in biofilm initiation in PA14. By the other side, cultures overexpressing &#963;x (ALB04) presented an altered lipopolysaccharide profile and a biphasic growth curve, reaching an early stationary phase followed by a growth resuming untill a second stationary phase. During the early stationary phase, most cells swells and dies, but the remaining cells return to wild type morphology and proceed to the second exponential phase of growth. This is not due to compensatory mutations, since cells collected from late points of the curve and diluted in fresh medium repeat this behavior. Although studies with strain PAO1 associate &#963;x with transcription of oprF, encoding the major nonspecific porin of Pseudomonas, under our experiments conditions with PA14, this porin expression is not induced by &#963;x overexpression. Thus, the effects observed in this overexpression cannot be attributed to OprF. Transcription of oprF in PA14 proved to be mainly controlled by the &#963;70-dependent promoter region instead of the &#963;x-dependent promoter region reported in the literature. Proteomic analyses were performed to investigate the elements involved in these effects of &#963;x overexpression, which revealed the induction of several enzymes involved in fatty acids biosynthesis. Cells overexpressing &#963;x exhibit a greater proportion of hexadecanoic (C16) and hexadecenoic (C16: 1) acids and anisotropy data show higher fluidity of the membrane (s). This work is the first report of an ECF sigma factor involved in lipid biosynthesis in P. aeruginosa.

O peso crescente do agronegócio na economia brasileira e as discussões em torno do modelo de desenvolvimento ideal para as áreas de expansão da fronteira agrícola têm sido um dos focos de debate relevantes para o futuro do País. Em um mundo ditado por um sistema econômico cada vez mais complexo, que exige respostas rápidas e adequadas à sua lógica, torna-se um imperativo distribuir a produção com eficiência, otimizando custos e valorizando os lugares circunscritos pela atuação das grandes empresas. A expansão dessas zonas, a partir da década de 1950, proporcionou uma complexidade territorial susceptível à análise geográfica, configurando um subsistema de circulação que estreita o vínculo de fluxos de partes das regiões Centro-Oeste e Norte ao restante do País. A proposta deste trabalho, então, parte da hipótese de que uma rede estruturadora de fluxos materiais, composta pelo entrelaçamento de vetores hidroviários e rodoviários em pontos nodais estratégicos, está se configurando para atender aos anseios da inserção e do crescimento de relevantes circuitos produtivos (principalmente soja, madeira, recursos minerais e pecuária bovina) nessas áreas, tidas até recentemente como desprovidas de movimento. À luz da teoria do espaço geográfico, procuramos direcionar o foco de nosso objeto de estudo para a fluidez territorial nessas \"porções\" do território nacional e analisamos a constituição das redes técnicas mediante uma periodização condizente com as transformações do meio geográfico. Por trás da instalação do meio técnico-científicoinformacional no período atual, desenha-se um quadro composto por elementos relacionados ao binômio psicosfera - tecnosfera que tenta justificar os eventos portadores de racionalidades exógenas ao lugar. Além de tentarmos demonstrar e explicar a configuração da rede supracitada (\"quadrilátero\" Manaus - Belém - DF/Goiânia - Porto Velho), como preocupação analítica paralela pretendemos refletir sobre a emergência de uma coesão territorial que possa fortalecer o ainda tênue perfil do mercado nacional. Assim, cabe perguntarmos de que maneira o avanço da fronteira agrícola está se desenvolvendo e a quem realmente beneficiará. / The emerging importance of agribusiness in the Brazilian economy and the discussions of the ideal development model for the expansion areas in the agricultural frontier has been one of the main topics of debate for the future of the country. In a world ruled by an economic system each time more complex which demands fast and adequate answers to its logic, it is imperative the efficient distribution of the production, optimizing costs and valuing the places circumscribed by the performance of the big companies. The expansion of these areas, which began in the 50\'s, has provided a territorial complexity susceptible to geographic analysis, configuring a circulation subsystem that narrows the bond of flows in parts of the Middle-West and North regions to the rest of the country. The proposal of this work starts from the hypothesis that a settling net of the material flows, composed by the interlacement of roads and river roads vectors in strategical points, is being set up to attend the insertion willings and the growth of productive circuits (mainly soy, wood, mineral resources and bovine cattle) in these areas, yet known as unprovided of movement. Based on the geographic space theory, we set the focus of our study object on the territorial fluidity in these \"portions\" of the national territory and analyze the constitution of the technical nets which faces a frequent schedule related with the transformations in the geographic environment. Behind this technician-scientific-informacional installation in the current period, a picture composed by elements related to the psycosphere - technosphere duality is drawn, wich try to justify the outside rationality events to the place. Besides trying to demonstrate and explain the configuration of the so mentioned net (\"quadrilateral\" Manaus - Belém - DF/Goiânia - Porto Velho), as a parallel analytical concern we intend to make a reflection on the emergence of a possible territorial cohesion that still fortifies the tenuous profile of the national market. Thus, it is suitable to ask how the advance of the agricultural frontier is being developed and who will it really benefit.

Moçambique é historicamente conhecido por ser um território de escoamento da produção do hinterland da África Austral, principalmente dos seus vizinhos África do Sul, Suazilândia, Zimbabwe e Malawi. A orientação de suas ferrovias, construídas ao longo do período colonial da região, mostra um território divido por três redes ferroviárias distintas, ligadas cada uma delas a um dos principais portos moçambicanos (Maputo, Beira e Nacala), mas não interligadas entre si, conformando assim um território aparentemente pouco integrado e voltado ao seu exterior. Ainda que parte importante da circulação contemporânea de mercadorias em Moçambique continue a respeitar essa lógica extravertida, desde a independência do país, em 1975, o governo moçambicano vem reunindo esforços na tentativa de integrar seu território e configurar uma economia eminentemente nacional. Tal tarefa cabe, no que tange à circulação de mercadorias, ao modal rodoviário, através de inúmeras rodovias (quase nunca pavimentadas) e de diferentes operadores de transporte (desde empresas até motoristas autônomos e transportadores informais), que atuam não apenas na circulação interna de mercadorias, mas também naquela voltada ao exterior. Assim, com base na proposta de Milton Santos de compreender o espaço geográfico a partir de suas dimensões técnicas e político-normativas, esta dissertação busca fornecer um quadro da circulação de mercadorias em Moçambique, a partir de sua estruturação histórica e dos dois sentidos que a orientam no período contemporâneo a circulação extravertida e a circulação interna. / Mozambique is historically known for being a drain region for the inner production of Southern Africa, especially its neighbors South Africa, Swaziland, Zimbabwe and Malawi. The course of its railways, built throughout the colonial period in the region, shows a territory divided by three distinct rail networks, each one linked to a major Mozambican port (Maputo, Beira and Nacala) but not interconnected. This constitutes a territory apparently little integrated and turned to its exterior. Even though an important part of the contemporary circulation of goods in Mozambique stills follows this extraverted logic, since the countrys independence in 1975 the Mozambican government has been making an effort to integrate its territory and set up a national economy. When it comes to the circulation of goods, this is a task that depends on road transport by means of numerous roads (mostly unpaved) and different transport operators (such as companies, autonomous drivers and informal carriers). These operate not only internally but also outwards. Thus, based on Milton Santos\' proposal to understand the geographical space through its technical and political-normative dimensions, this dissertation attempts at providing a framework for the circulation of goods in Mozambique regarding its historical constitution and the two directions that orientate it in the contemporary period: the extraverted and the internal circulation.

Os condomínios empresariais são produtos imobiliários do período contemporâneo capazes de influenciar a decisão locacional de atividades econômicas, em especial, indústria e serviços. Esta dissertação tem como objetivo permitir a compreensão da relação entre a produção imobiliária e a localização industrial. Com base em entrevistas com agentes imobiliários responsáveis por estes empreendimentos e representantes de empresas que se instalaram nos condomínios, foi possível analisar alguns aspectos desta relação. A necessidade de caracterizar este fenômeno exigiu o monitoramento da produção e dispersão dos condomínios empresariais nas áreas metropolitanas do Estado de São Paulo, que permitiu ser feito seu mapeamento, bem com a elaboração de sua definição, classificação e periodização. Estes empreendimentos são expressão de um novo processo no que tange à localização de indústrias, pois implica considerar o agente imobiliário, interessado em influenciar estas decisões, diferentemente de momentos anteriores, quando estavam alicerçadas apenas no âmbito microeconômico ou a partir da ação do Estado. Participando deste processo, os condomínios intensificam o uso corporativo do território, já que permitem flexibilizar as localizações e dispõem de funcionalidades que tendem a facilitar a ação das empresas. Dessa forma, apontam para fatores de localização contemporâneos que permitem pensar a relação entre as empresas e o território no período técnico-científico-informacional. Além disso, estes empreendimentos são expressão de um processo de urbanização no qual as formas são fragmentadas por meio do estabelecimento de espaços exclusivos e de acesso controlado, resultando na conexão muito mais forte com o exterior por meio da elevada fluidez territorial de que dispõem, do que em relação à sua vizinhança adjacente, o que evidencia contradição na tendência da produção de espaços exclusivos em detrimento do espaço público. / Business condominiums are real estate products of the contemporary period that can be an influence to the locational decisions about economic activities, in particular to the industry and services activities. This dissertation aims to enable understanding the relation between real estate production and industrial location. Based on interviews with real estate agents responsible for these enterprises and representatives of companies that settled in the condominiums, was possible to analyze some aspects of this relationship. The need to characterize this phenomenon required the monitoring of production and dispersion of these enterprises in metropolitan areas of São Paulo, has allowed it mapping as well as the preparation of its definition, classification and periodization. These enterprises are an expression of a new process regarding industries location, because it implies considering the real estate agent, interested in influencing these decisions, unlike in previous occasions when they were grounded in microeconomic scope or only from the state action. By participating in this process, the condominiums enhance the corporate use of the territory, as they allow flexible locations and have features that tend to facilitate the actions of companies. Thus, point to contemporary location factors that allow the understanding of the relation between companies and territory in the technical-scientific-informational period. Moreover, these enterprises are an expression of urbanization process in which the forms are fragmented through the establishment of enclosed spaces and controlled access, resulting in much stronger connection with the outside through the territorial fluidity at their disposal, than in respect to their surrounding environment, which shows contradiction in the trend of production of exclusive areas at the expense of public space.

Magister Philosophiae - MPhil / Instability in the administration of municipalities is a particularly serious problem in the Western Cape because of its large number of coalition councils. Coalitions have led to frequent changes in local governance and to constant changes in political and administrative leadership. Due to the fluidity in local government, the politics of the day have become synonymous with back-stabbing, secret agreements and arrangements between politicians and political parties wishing to align themselves in such a way as to gain control of the councils.1 A municipality is required by the Constitution2 to structure and manage its administration and budgets, budgeting and planning processes so as to give priority to the basic needs of the community and to promote the social and economic development of the community.3 Legislation further prescribes that “a municipality must within its administrative and financial capacity establish and organize its administration in a manner that would enable the municipality to establish clear relationships, facilitate coordination, cooperation and communication between (i) its political structures and political office bearers and its administration; (ii) its political structures, political office bearers and administration and the local community”.4 It may therefore be argued that a municipality subscribing to the abovementioned prescripts should be functioning effectively. However, in practice continuous administrative and political instability adversely impacts on a municipality‟s capacity to provide service delivery to the community. The 2006 local government elections resulted in only four out of 30 municipalities in the Western Cape having a single party with more than 50% of the seats (outright majority). The remaining 26 municipalities were governed by coalitions of two or more parties. In 2001, Parliament introduced floor-crossing legislation which allowed Members of Parliament, Members of Provincial Legislatures and local government councillors to change their political party (or form a new party) and retains their seats when they did so.5 As a result of the 2007 floor-crossing legislation, the number of municipalities with an outright majority increased to 7. Power changes continued to occur even after the 2007 floor-crossing as a result of by-election outcomes or new internal coalition arrangements. Coalition government in the Western Cape remains a reality following the 18 May 2011 local government elections: the Democratic Alliance won 12 municipalities outright, the African National Congress won 1, and in 12 municipalities there was no outright winner. Of the 12 last-mentioned municipalities, 7 municipalities produced hung municipalities, i.e. Bitou, Witzenberg, Laingsburg, Hessequa, Theewaterskloof, Matzikama and Prince Albert. / South Africa

Products manufactured by aluminium casting have become very popular and already replaced many parts that were once produced by iron and steel casting. This trends upwards especially in the automotive industry as it has become extremely important to reduce vehicle weight due to environmental requirements and economical aspects. This popularity of aluminium alloys could be ascribed to their light weights and many other advantages including excellent castability, good corrosion resistance, good thermal and electrical conductivity, good machinability, low melting temperatures and minimal gas solubility with the exception of hydrogen. The most important alloy group among casting alloys is Aluminium Silicon (Al - Si).   Al-Si alloys must undergo a specific melt treatment procedure prior to casting. This treatment consists of several steps including degassing of hydrogen, grain refinement and eutectic modification. The aim of this study is to make an assessment of the metal treatment process of an (Al-Si) casting alloy at Unnaryd Modell AB for the purpose of improving the melt conditions and thus the quality of the final product. A rotary degasser provided by Foseco is also tested instead of the traditional tablet degassing method to see if this technique would result in any significant improvement of the melt quality. The results show that Unnaryd modell AB follows a proper treatment routine. It shows moreover that the rotary degassing is superior to the tablet degassing in many aspects including the level of degassing achieved, time efficiency, environmental consideration and personnel security.

Cold adaptation in stationary phase in Bacillus subtilis One of the most important abiotic factor which influences life of bacterial cells is the ambient temperature. A decrease of this temperature is usually accompanied usually with the loss of the fluidity of bacterial cytoplasmatic membrane. While the mechanisms of the responses to the cold shock during the exponential phase of growth are well known for Bacillus subtilis, the responses of stationary phase cells had not been studied yet (despite the stationary phase is the most common state of microorganism in the nature). There are two independent mechanisms which restores much needed fluidity in Bacillus subtilis - short-term adaptation and long-term adaptation. Short-term adaptation is based on the function of fatty acid desaturase coded by des gene. Long-term adaptation relies on the change in ratio of iso- and anteiso- branched fatty acids. In this work we examinated membrane adaptation during stationary phase under two different conditions, namely under cultivation at stable low temperature and after cold shock. The highest activity of Pdes was observed for cultivation at 25 řC and for the cold shock applied from cultivation in 37 řC to 25 řC. Anisotropy measurements and fatty acids analysis were also performed. Results indicated, that the...