The objectives of this research were to select new strawberry clones for the estate of Rio Grande do Sul, Brazil, and to develop methods of multiplication to obtain disease-free transplants with high physiological quality. Five advanced strawberry clones from the Breeding Program and two controls were evaluated in an annual hill system in low tunnels from April to December, 2006. A closed soilless system was developed, based on a growing bed with substrate over a cement tile. A nutrient solution was delivered from a reservoir to the upper end of the tile and drained off back by gravity. An inert substrate (sand) and an organic substrate (Plantmax®) and two advanced strawberry clones were tested. In another experiment fruit yield of plug transplants of different sizes was compared to that of bare-root transplants. Plug transplants were produced rooting runner tips in plastic trays with different volumes of organic substrate. Bare-root transplants were produced in the closed soilless growing system described above. Clones LBD 15.1, LBH 27.2, LBD 35.2 and LBG 121.4 were identified as having potential to be used in the estate of RS. These clones combine earliness, high yield and fruit quality, high content of bioactive compounds and resistance to diseases. A high number of healthy bare-root and runner tips with high quality were obtained with both substrates and both clones. A higher early fruit yield during fall and winter was obtained with plug transplants. Both plug and bare-root transplants reached a high total yield. It was concluded that selected strawberry clones of this research can be recommended to substitute commercial cultivars now planted in the RS or used in combination with them and that disease-free bare-root transplants and runner tips for plug transplants, both with high physiological quality can be produced in the closed soilless system, providing a sustainable alternative for nurseries. / Os objetivos deste trabalho foram selecionar novos clones de morangueiro para o RS e desenvolver um sistema de multiplicação para a obtenção de mudas com alta qualidade fisiológica e sanitária. Foram avaliados cinco clones avançados do Programa de Melhoramento e duas testemunhas, em túneis baixos, durante os meses de abril e dezembro de 2006. Para a multiplicação foi utilizado um sistema fechado sem solo, baseado em um leito de cultivo com substrato sobre telhas de fibrocimento. A circulação da solução nutritiva foi feita a partir de um reservatório até a extremidade mais alta da telha, drenando por gravidade. Como substrato, testou-se a areia na categoria inerte e o Plantmax® na categoria orgânica, com dois clones. Em outro experimento foi comparada a produtividade de mudas com torrão de diferentes tamanhos e com raízes nuas. As mudas com torrão foram produzidas a partir de pontas de estolão enraizadas em bandejas com diferentes volumes de substrato orgânico. As mudas com raízes nuas foram provenientes do sistema fechado sem solo. Foram identificados os clones LBD 15.1, LBH 27.2, LBD 35.2 e LBG 121.4 com potencial para serem cultivados no RS. Esses clones combinam alta produtividade precoce e total, qualidade de fruta, conteúdo de componentes bioativos na fruta e resistência às doenças. Um alto número de mudas e pontas de estolão sadias e de alta qualidade foi obtido no sistema fechado sem solo com ambos os substratos e clones. As mudas com torrão apresentaram maior produtividade precoce no outono e inverno. Tanto as mudas com torrão como as de raízes nuas alcançaram elevada produtividade total. Concluiu-se que os novos clones selecionados podem ser indicados em substituição ou em combinação com as cultivares atualmente em uso no RS e que o sistema fechado sem solo é uma alternativa sustentável para ser empregada na produção de mudas com raízes nuas e de pontas de estolão para mudas com torrão.

Made available in DSpace on 2015-03-26T13:40:04Z (GMT). No. of bitstreams: 1 texto completo.pdf: 721255 bytes, checksum: 77be2e89e014b1cf674143a721d65f78 (MD5) Previous issue date: 2005-03-17 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / This research had the objective of evaluating the organic fertilization effects on the production, quality, post-crop conservation and acceptability of fruits of the yellow passion fruit plant (Passiflora edulis, Sims f. flavicarpa Degener). The experiment was established in March 2004, in a red yellow Latosol of Sementeira Experimental Farm - UFV, in Visconde do Rio Branco, MG. The experimental design was completely randomized with 4 repetitions. The treatments were formed by three types of fertilizations applied in the orchard: Mineral (MIN) suitable for the culture (control); Organic (ORG), equivalent to the potassic fertilization suitable for the culture and 2 times organic (2XORG), where was used the ORG treatment double dose. The organic fertilizer used was the bovine fertilizer. In May 2005, fruits from each treatment were transported to the Agro-ecology Laboratory UFV, for quality and post-crop conservation evaluation. In June 2005 fruits from each treatment were selected and transported to the Sensorial Analysis Laboratory of the Food Technology Department UFV, for juice acceptability and fruits appearance evaluation. The yellow passion fruit plant production fertilized with 2XORG and MIN was 26% superior when compared to the plants fertilized with ORG production. The fruits classification (mass and number of defects), was not influenced by the fertilizations. The fruits mass loss from plants fertilized with bovine manure (ORG and 2XORG) was smaller than fruits from plants fertilized with MIN. The fruits fertilized with bovine manure chemicals characteristics (pH, SST, ATT, Vitamin C) were similar to the one of the fruits from plants fertilized with MIN. The fruits juice acceptability from the 3 fertilizations was similar, however, with relation to the appearance, the acceptability was larger for the fruits of the treatment 2XORG. / A pesquisa teve o objetivo de avaliar os efeitos da adubação orgânica sobre a produção, qualidade, conservação pós-colheita e aceitabilidade de frutos do maracujazeiro amarelo (Passiflora edulis, Sims f. flavicarpa Degener). O experimento foi estabelecido em março de 2004, num latossolo vermelho amarelo da Fazenda Experimental da Sementeira UFV, situada em Visconde do Rio Branco, MG. O delineamento experimental foi inteiramente ao acaso com 4 repetições. Os tratamentos foram formados por três tipos de adubações aplicados no pomar: Mineral (MIN) indicada para a cultura (testemunha); Orgânica (ORG), equivalente à adubação potássica indicada para a cultura e 2 vezes orgânica (2XORG), onde foi utilizado o dobro da dose do tratamento ORG. O adubo orgânico utilizado foi o esterco bovino. Em maio de 2005, frutos provenientes de cada tratamento foram transportados ao Laboratório de Agroecologia da UFV para avaliação da qualidade e conservação pós- colheita. Em junho 2005 foram selecionados frutos de cada tratamento e transportados ao Laboratório de Análise Sensorial do Departamento de Tecnologia de Alimentos da UFV para avaliação da aceitabilidade do suco e aparência dos frutos. A produção do maracujazeiro amarelo adubado com 2XORG e MIN foi 26% superior quando comparada com a produção de plantas adubadas com ORG. A classificação dos frutos (massa e No de defeitos), não foi influenciada pelas adubações. A perda de massa dos frutos provenientes de plantas adubadas com esterco bovino (ORG e 2XORG) foi menor do que frutos provenientes de plantas adubadas com MIN. As características químicas (pH, SST, ATT, Vitamina C) dos frutos adubados com esterco bovino foram semelhantes às dos frutos de plantas adubadas com MIN. A aceitabilidade do suco dos frutos provenientes das 3 adubações foram semelhantes, no entanto, com relação a aparência, a aceitabilidade foi maior para os frutos do tratamento 2XORG.

Made available in DSpace on 2016-12-23T14:37:43Z (GMT). No. of bitstreams: 1 Sara Coser Morra.pdf: 1157047 bytes, checksum: ca26cc99cfaca69da596f91375dc0dcb (MD5) Previous issue date: 2012-07-12 / A goiabeira (Psidium guajava L.) é uma das fruteiras de maior importância econômica da família Myrtaceae. O Brasil é um dos maiores produtores de goiaba do mundo, sendo esta uma cultura potencial em expansão e rentabilidade. A polinização cruzada da espécie e a existência de pomares heterogêneos de propagação seminal resultam em variabilidade, permitindo a seleção de genótipos para o melhoramento da cultura. O objetivo deste trabalho foi estudar a diversidade genética em genótipos de P. guajava selecionados em pomar de origem seminal e cultivares por características morfológicas e químicas de qualidade de fruto, também associar dados de conteúdo de DNA nuclear (2C), cariótipo, morfológicos e de marcadores moleculares microssatélites. Verificou-se a existência de divergência entre as Cortibel com relação às características de fruto, com genótipos apresentando performance superior e genótipos com desempenho semelhante à cultivares, potenciais para uso em hibridações ou como cultivares. As análises cariotípica e de conteúdo de DNA nuclear (2C) mostraram que os genótipos possuem um genoma estável, pequeno e diplóide e características cariotípicas relacionadas a grupos ancestrais de angiospermas. O dendrograma UPGMA baseado em dados morfológicos e SSR evidenciaram diversidade entre os genótipos, com melhor discriminação pelos dados de SSR. Como a maioria dos genótipos mostraram similaridade morfológica para as características de frutos, aliada a dissimilaridade molecular, estes se mostraram interessantes para o uso em hibridações em programas de melhoramento. O conjunto de dados gerados contribuiu para expandir o conhecimento sobre o genoma e a diversidade genética em P. guajava. Também é importante na estruturação de programas de melhoramento para a cultura, além de contribuir para estudos evolutivos / Guava (Psidium guajava L.) is one of the most economically important fruit crop from Myrtaceae family. Brazil is one of the largest producers of guava in the world, which is a potential crop in growth and profitability. Cross-pollination of the species and the existence of heterogeneous seminal propagation orchards result in variability, allowing the selection of genotypes for crop improvement. The aim of this study was to evaluate genetic diversity between P. guajava L genotypes selected from seminal origin orchard and cultivars, by morphological and fruit quality chemical characteristics, also associate data from nuclear 2C-value, karyotypic, morphological and simple sequence repeat (SSR) marker. There were divergences between Cortibel selections by fruit characteristics, with genotypes showing superior and similar performance when compared with cultivated genotypes, potential for use in hibridation and as cultivars. Karyotype and nuclear 2C-value analyses showed that all genotypes have a stable and very small diploid genome (2n = 2X = 22; 2C = 0.95 pg), and karyotypic characteristics related to ancestral angiosperm groups. UPGMA dendrogram based on morphological and SSR data evidenced diversity among the genotypes, with better discrimination by SSR data. Since most genotypes showed morphological similarity for fruit characteristics, combined with molecular dissimilarity, the use of these genotypes in hybridation breeding programs could be of interest. The obtained data set contributed to expand the knowledge about genome and genetic diversity of P. guajava. Also are important to structure crop improvement programs and contribute to evolutionary approaches

Made available in DSpace on 2014-08-20T14:33:03Z (GMT). No. of bitstreams: 1 dissertacao_gabriel_natchigall_marques.pdf: 1272294 bytes, checksum: bc35ce0c7d238bbde2ae6127a45b29a6 (MD5) Previous issue date: 2013-02-18 / Mini watermelons production in hydroponics and greenhouse conditions can be a viable alternative for obtaining high fruit yield and quality jointly with a better productive resources use and reduced environmental impact. In this sense, the analysis of growth and the study on water consumption are tools that can be used to produce knowledge and information regarding crop behavior and very important for a better irrigation management, allowing to choose the most suitable genotype to the proposed crop system. Thus, two experiments were conducted with mini watermelon crop in hydroponic system under greenhouse and spring-summer crop-season at Universidade Federal de Pelotas , in Capão do Leão, RS . The first one aimed to characterize the growth dynamics of mini watermelon hybrid genotypes Beni Kodama® , Extasy® , Ki Kodama® and Taiyo® at 11 sampling dates (0, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 days after transplantation). Based on dry mass (DM) and leaf area data, it can be concluded that the genotypes have expressed growing tendency in relation to the accumulation of total plant DM, with a sigmoidal type growth curve. Genotype Extasy has shown higher vegetative growth from the half to the end of the crop cycle and lower intensity of fruit growth in the early and higher intensity of fruit growth in the end of the crop cycle than others. In relation to the fruit DM production, all genotypes presented a linear response to the time course of the crop cycle. Despite differences among fruit growth rates during the time course of the crop cycle, all genotypes showed similar patterns of fruit DM production at the end of the crop-season. For all genotypes, the crop relative growth rate decreased in relation to the age of the plant. Concerning the plant dry mass partitioning, leaves were the main drains of assimilates of the plant until the middle of the crop cycle. Fruits comprised from 1 to 5 % of the plant DM production at the beginning of fruiting and from 70 to 76 % of the plant DM at the end of the crop cycle. The second experiment aimed to characterize the duration of plant phenological phases, quantify the water consumption, determine the water use efficiency (WUE), as well as fruit yield and quality of two mini watermelon hybrids, 'Rapid Fire®' and 'Smile® . The obtained results indicated that 'Rapid Fire®' and 'Smile® showed similar total and daily volume of water consumption during the crop cycle, WUE, fruit yield and fruit chemical characteristics. For both genotypes, the maximum daily water consumption was 1.5 liter plant-1 day-1; the total water consumption was about 73-76 liters plant-1 and 80 % of the demand occurred during phenological stage III (maximum fruiting). The hybrids 'Rapid Fire®' and 'Smile®' showed fruit yield of 12.56 and 9.37 kg m-2, respectively, and an average solid soluble content of 10.3oBrix. 'Rapid Fire®' highlighted by its very high WUE for fruit production, 52.77 g L-1 on average. 'Rapid Fire®' and 'Smile® are potentially high fruit yielding genotypes, which present a high WUE and chemical characteristics compatible to the market requirements. / A produção de minimelancias em cultivo hidropônico sob ambiente protegido pode ser uma alternativa viável para a obtenção de alto rendimento e elevada qualidade de frutos, além da melhor otimização dos recursos produtivos e do reduzido impacto ambiental. Neste sentido, a análise do crescimento e o estudo do consumo hídrico são ferramentas que podem ser utilizadas para produzir conhecimentos e informações referentes ao comportamento da cultura e importantes para o manejo da irrigação, permitindo escolher os genótipos mais adaptados ao sistema de cultivo proposto. Assim, dois experimentos foram realizados com a cultura da minimelancia em sistema hidropônico e ambiente protegido em ciclo de primavera/verão no Campus da Universidade Federal de Pelotas, no município de Capão do Leão, RS. O primeiro experimento objetivou caracterizar a dinâmica do crescimento dos genótipos Beni Kodama® , Extasy® , Ki Kodama® e Taiyo® em 11 épocas de avaliação (0, 7, 14, 21, 28, 35, 42, 49, 56, 63 e 70 dias após o transplante). Com base nos dados de massa seca (MS) e área foliar, concluiu-se que os genótipos expressaram tendência crescente em relação ao acúmulo de MS total da planta, com crescimento do tipo sigmoidal. O genótipo Extasy apresentou maior crescimento vegetativo aéreo a partir da metade do ciclo de cultivo, com menor intensidade de crescimento dos frutos no inicio e maior intensidade ao final do ciclo de cultivo que os demais. Em relação à produção de MS de frutos, todos os genótipos seguiram comportamento linear em função do período de cultivo e as diferenças na taxa de crescimento absoluto de frutos não acarretaram em diferenças no acúmulo final de massa seca nestes órgãos. Para os quatro genótipos, a taxa de crescimento relativo da cultura decresceu em função da idade da planta. No que diz respeito à dinâmica de partição da MS na planta, até a metade do ciclo de cultivo, as folhas foram os principais drenos de assimilados. Os frutos representaram de 1 a 5 % da MS da planta no inicio da frutificação e de 70 a 76 % da MS da planta no fim do ciclo. O segundo experimento objetivou caracterizar a duração das fases fenológicas, quantificar o consumo hídrico, determinar a eficiência no uso da água (EUA), bem como o rendimento e qualidade de frutos dos genótipos Rapid Fire® e Smile® . Os resultados obtidos indicam que os genótipos apresentaram similaridade em relação ao volume total e diário de água consumida durante o ciclo de cultivo, à EUA, ao rendimento e às características químicas dos frutos. Para ambos os genótipos, a demanda máxima diária de água foi de 1,5 litro planta-1 dia-1; a demanda total foi de cerca de 73-76 litros planta-1 e 80% desta ocorreu durante a fase fenológica III (plena frutificação). Os híbridos Rapid Fire® e Smile® apresentaram rendimento de frutos de 12,56 e 9,37 kg m-2, respectivamente, e média do teor de sólidos solúveis totais de 10,3oBrix. Rapid Fire® salientou-se pela elevada EUA na produção de frutos, da ordem de 52,77 g L-1. Assim, Rapid Fire® e Smile® são genótipos potencialmente de alto rendimento de frutos, com alta eficiência no uso da água e com características químicas compatíveis às exigências do mercado.

Made available in DSpace on 2014-08-20T13:59:11Z (GMT). No. of bitstreams: 1 dissertacao_tiago_zanatta_aumonde.pdf: 573942 bytes, checksum: 3c1ab4ac502409888cd80ead135d58f4 (MD5) Previous issue date: 2010-03-02 / The work was carried in the field between 2008 and 2009 at the Federal University of Pelotas and aimed to evaluate the mini watermelon cultivar Smile ® grafted and ungrafted. In the seedling stage were evalueted the survival rates and lignification. The rootstocks that provided the best results were transplanted to the field with ungrafted plants and where analyzed the growth, dry matter partitioning, production and fruit quality. The access Irai and C314, in the seedling stage, provided the best results. In the field, plants ungrafted obtained more accumulation total of dry matter, produticon rate of dry matter, relative growth rate, net assimilation rate, relative growth rate of leaf area, leaf area ratio and leaf weight. But, the specific leaf area was higher in grafted plants. Allied to this, the maximum plant height was 1.5 meters to 49 days after transplanting (DAT) in grafted plants and 42 DAT in ungrafted. The leaf dry matter (Wf) were increased. Regarding Wf, increased to 70 DAT in grafted plants (52.91 g m-2) and to 63 DAT in ungrafted (71.55 g m-2). Concomitantly, the number of leaves increased to 63 DAT. Regarding the dry matter of stem (Wc), the maximum was 6.56 g m-2 in grafted plants and 7.85 g m-2 in ungrafted, at 70 DAT. The dry matter of fruit (Wfr) started to compose the total dry matter from 42 DAT and was growing by the end of the cycle (70 DAT). For the grafted plant, Wfr maximum was 47.29 g m-2 and to ungrafted plants was 57.20 g m-2. The growth rate of leaf (Cf) increased until the end of the cycle with the Cf of 1.31 g m-2 d-1. Already, in ungrafted the Cf maximum was of 1.92 g m-2 d-1, at 49 DAT. The production rate of dry matter of stem (Cc) in the grafted watermelon was the maximum of 0.72 g m-2 d-1 (70 DAT), and grafted plants was 0.48 g m-2 d-1 (56 DAT). The growth rate maximum of fruit (Cf) was 3.66 g m-2 d-1 (70 DAT), in grafted plants. Already, the Cf in grafted plants was 2.97 g m-2d-1 (56 DAT). Grafted plants produced less than ungrafted, however, provided the best color of pulp and higher amount of phenols in the fruits did not differ in other variables when compared to ungrafted showing the potential of this material as a rootstock for hybrid mini watermelon Smile®. / O trabalho foi realizado a campo entre no período de 2008 a 2009 na Universidade Federal de Pelotas e objetivou avaliar a cultivar de mini melancia Smile® na condição enxertada e não enxertada (pé-franco). Na fase de muda foram avaliados os índices de pega e lignificação. O porta-enxerto que proporcionou os melhores resultados foi transplantado para o campo juntamente com pé-franco onde foram analisados o crescimento, a partição de matéria seca, a produção e a qualidade dos frutos. Na fase de muda, os acessos Irai e C314 proporcionaram os melhores resultados. Na fase de campo, pé-franco obteve maior acumulo de matéria seca total, taxa de produção de matéria seca, taxa de crescimento relativo, taxa assimilatória líquida, taxa de crescimento relativo de área foliar, razão de área foliar e razão de massa foliar. Entretanto, a área foliar específica foi superior em plantas enxertadas. Aliado a isso, altura a altura máxima foi de 1,5 metros aos 49 dias após o transplante (DAT) em plantas enxertadas e aos 42 DAT em pé-franco. A matéria seca foliar (Wf) sempre foi crescente. No que tange a Wf, foi crescente até os 70 DAT em plantas enxertadas (52,91 g m-2) e até os 63 DAT em pé-franco (71,55 g m-2). Concomitantemente, o número de folhas aumentou até os 63 DAT. No que concerne a matéria seca de caule (Wc), o máximo foi de 6,56 g m-2 em plantas enxertadas e de 7,85 g m-2 em pé-franco, aos 70 DAT . A matéria seca dos frutos (Wfr) iniciou a compor a matéria seca total a partir dos 42 DAT e foi crescente até o final do ciclo de cultivo (70 DAT). Para planta enxertada o Wfr máximo foi de 47,29 g m-2 e para pé-franco foi de 57,20 g m-2. A taxa de crescimento de folha (Cf) foi crescente durante todo o ciclo com o Cf máximo de 1,31 g m-2 d-1. Já, em pé-franco o Cf máximo foi de 2,16 g m-2 d-1, aproximadamente aos 42 DAT. Quanto a taxa de produção de matéria seca de caule (Cc), em melancia enxertada o Cc máximo foi de 0,72 g m-2 d-1 (70 DAT) e, em pé-franco o Cc máximo foi de 0,48 g m-2 d-1 (56 DAT). A taxa de crescimento máxima de fruto (Cfr) em planta enxertada foi de 3,66 g m-2 d-1 (70 DAT). Já, a Cfr pé-franco foi de 2,97 g m-2d-1 (56 DAT). Plantas enxertadas produziram menos do que pé-franco, entretanto, proporcionaram melhor coloração de polpa e maior quantidade de fenóis totais nos frutos, não diferindo nas demais variáveis analisadas quando comparadas ao pé-franco mostrando a potencialidade desse material como porta-enxerto para o híbrido de mini melancia Smile®.

Le dernier rapport du GIEC confirme clairement des projections climatiques prévoyant une augmentation des températures et de leur variabilité à la fin du XXIème siècle. Les effets de ces changements sur la production et la qualité des fruits ont été étudiés dans le cadre du projet CAQ40(INRA). Les expérimentations menées sur des pêchers en pot placés sous différents climats (témoin,+2°C et +5°C), ont permis d’identifier les processus (phénologie, développement, croissance,photosynthèse, métabolisme) les plus sensibles à l’élévation des températures et leurs conséquences sur le développement et la composition des fruits et la pérennité de la production.L’élévation de température a augmenté la demande climatique entraînant une diminution du potentiel hydrique des arbres, ce qui a pu entrainer une concentration des composés d’intérêt dans le fruit. La réduction du taux de photosynthèse des feuilles dans des conditions de forte température est liée à une inhibition de la photosynthèse par les températures élevées et à un contrôle stomatique lié au statut hydrique.Après floraison, l’élévation de température accélère la croissance végétative, induisant une mise en place de la surface foliaire anticipée. Cette croissance végétative précoce a eu pour conséquence (i) une dynamique très rapide d’élongation des axes en pousse longue (ii) une dominance apicale plus marquée, (iii) une diminution de la ramification des axes axillaires,contrairement aux traitements thermiques plus tardifs qui ont eu un effet défavorable sur l’initiation et la différenciation des bourgeons floraux réduisant le potentiel de production l’année suivante.En outre, l’augmentation de température après floraison a entraîné un raccourcissement très marqué de la durée de croissance du fruit, avec une date de récolte anticipée de près de 3semaines. Ce raccourcissement de la durée de croissance du fruit a entraîné une diminution du flux entrant de carbone dans le fruit, pénalisant sa croissance et sa qualité. Le climat très différent entre les deux années expérimentales a entrainé une forte variabilité de la composition des fruits entre les deux années d’expérimentation. En 2014, une élévation de température précoce ou continue a conduit à récolter des fruits de petites tailles ayant des concentrations et des teneurs en saccharose plus importantes. En 2015, la durée de développement entre floraison et maturité était encore plus courte qu’en 2014 ce qui pouvait être lié au climat plus chaud de 2015 et les fruits ont été moins sucrés et plus acides que ceux de 2014. Toutefois, l’élévation des températures en fin de développement en 2015 a augmenté les teneurs et les concentrations en hexoses et en sorbitol liées à un effet concentration mais également à un effet sur le métabolisme. L’élévation des températures en milieu et en fin de cycle a également favorisé l’accumulation d’acide malique et citrique. Les fortes températures n’ont pas eu beaucoup d’effet sur la vitamine C et ont soit augmenté ou diminué les teneurs en composés phénoliques. Les effets d’une élévation de la température sur le métabolisme sont donc très dépendants du stade de développement du fruit.L’utilisation de modèle à l’échelle du fruit (Virtual Fruit) et à l’échelle de l’arbre (QualiTree)pourrait permettre de simuler à la fois l’effet de l’environnement et des pratiques culturales sur la croissance et la qualité du fruit, et donner une vision plus intégrée du fonctionnement de la plante sous contraintes environnementales. / The latest IPCC report clearly confirms the climate projections for increasing temperaturesand their variability at the end of the 21st century. The effects of climate changes in fruit yield andquality have been studied in a project funded by INRA (project CAQ40, Metaprogramme ACCAF).Experiments carried out on potted peaches placed in different climates (control, +2 ° C and + 5 ° C),allowed the identification of the processes (phenology, development, growth, photosynthesis,metabolism) most sensitive to rising temperatures and their consequences on the development andcomposition of fruits and the sustainability of production.Higher temperature has increased the demand for water, leading to a decrease in the waterpotential of the trees, which may have led to a concentration of the compounds of interest in thefruit. The reduction of leaf photosynthesis under high temperature conditions was related to theinhibition of photosynthesis by high temperatures and stomatal control related to water status.After flowering, the rise in temperature accelerates the vegetative growth, triggering a more rapidestablishment of leaf area. This early vegetative growth resulted in: (i) very rapid dynamics ofelongation of the axes in long shoot (ii) a more pronounced apical dominance, (iii) a decrease in theaxillary axial branching. In contrast the later heat treatment had an adverse effect on the initiationand differentiation of floral buds reducing the production potential in the following year.In addition, the increase in temperature after flowering resulted in a marked shortening ofthe fruit growth period, with an expected harvest date almost 3 weeks earlier. This shortening offruit growth duration has led to a decrease in the flow of carbon entering the fruit, penalizing itsgrowth and quality. The very different climates between the two experimental years resulted in ahigh variability in fruit composition between the two years of experimentation. In 2014, increasedtemperature during the early stage of fruit development or continuously led to the harvest ofsmaller fruit with higher concentrations and higher sucrose content. In 2015, the time durationbetween flowering and maturity was even shorter than in 2014, which could be linked to thewarmer climate of 2015. In 2015 fruits were less sweet and acidic than those of 2014. However, therise of temperatures at the end of fruit development in 2015 increased the levels andconcentrations of hexoses and sorbitol; this increase was partially due to a concentration effect butalso to an effect on fruit metabolism. Increased temperatures in the middle and at the end of fruitdevelopment also favoured the accumulation of malic and citric acid. The high temperatures did nothave much effect on vitamin C and either increased or decreased the levels of phenolic compounds.The effects of an increase in temperature on the metabolism are therefore very dependent on thestage of fruit development.The use of a Fruit‐scale model and a tree‐level (QualiTree) model could simulate both the effect ofthe environment and cultural practices on the growth and quality of the fruit, and give a moreintegrated view of the plant's functioning under environmental constraints.

En horticulture, les effets négatifs du déficit hydrique (WD) sur le rendement peuvent être en partie compensés par des effets positifs sur la qualité des fruits et la stimulation des défenses de la plante face à d’autres stress. L'objectif de la thèse était d'explorer un certain nombre de pistes permettant d'évaluer les effets positifs et négatifs du WD, et d'appréhender les facteurs de variations. Après avoir établi un état des connaissances actuelles des effets du WD sur la qualité des fruits, plusieurs questions ont été abordées: 1) quels sont les effets de WD appliqués à des stades précis du développement de la plante ou du fruit, 2) existe-t-il une mémoire du stress en cas d’alternance de période de WD et de récupération, 3) les défenses de la plante sont-elles stimulées par un WD lors d'une infection par un pathogène, et enfin 4) quelle est la variabilité génétique associée aux réponses au WD au travers des objectifs précédents. Pour cela, plusieurs expérimentations ont été réalisées en serres et en phytotrons. Les parents de la population MAGIC de la tomate (ayant une forte variabilité allélique) ont été étudiés, ainsi que deux tomates commerciales (pour l’étude de l’interaction WD – Botrytis cinerea). Les mécanismes de réponses induits lors du WD ont été caractérisés aux stades végétatif et reproductif à l’échelle de la plante, puis lors des phases de développement du fruit (division cellulaire, expansion cellulaire et maturation). Les stades végétatif et reproductif se distinguent par les mécanismes de gestion du stress oxydatif engendré par le WD. Les plantes végétatives seraient plus affectées au niveau des photosystèmes II alors que les plantes reproductives modulent plutôt leur besoin en eau et sont plus sensibles à l’approche des photosystèmes I. L’impact du WD sur la qualité des fruits diffère en fonction de leurs phases de développement. De forts effets génotypes ont été observés avec une amélioration de la qualité dite gustative (par rapport aux teneurs en sucres et acides) pour un génotype dont les caractéristiques initiales étaient plus faibles que le second génotype testé. L’alternance de périodes de WD et de récupérations constitue un bon compromis entre économie d’eau et adaptation des plantes (via une bonne gestion du stress oxydatif). Cette alternance de stress a des effets neutres à positifs sur les teneurs en sucres des fruits en fonction des génotypes et des réponses très variables pour les teneurs en acides organiques, caroténoïdes et acide ascorbique en fonction des génotypes. L’interaction entre un WD et B. cinerea génère une diminution du ratio C/N associé à une stimulation des défenses de la plante. Cependant l'interaction favorise le développement du pathogène dans les tiges. Cet effet délétère de l'interaction semble lié aux régulations hormonales mises en jeu avec la stimulation de l'ABA (par le WD) et de la voie SA (par B. cinerea et indirectement par le WD) qui sont antagonistes aux voies JA/ET de réponse aux pathogènes nécrotrophes. Toutefois, un effet positif a été observé pour les infections sur feuilles détachées qui constituent un instantané des défenses de la plante, et ce, uniquement pour les plantes déjà infectées sur tige. Ces résultats suscitent de nouvelles interrogations sur la stimulation des défenses de la plante. Ainsi, le WD permet une augmentation du pool de sucres solubles, sans impact notable sur le rendement, avec des effets variables sur les composés secondaires. Le WD permet également dans une certaine mesure de stimuler les défenses de la plante mais de nouvelles recherches sont nécessaires. Enfin, la variabilité génétique des réponses à l’échelle de la plante et du fruit est importante et interagit avec le WD. / In horticulture, yield losses associated to water deficit (WD) could be compensated by positive effects on fruit quality and stimulation of plant to others stresses. The aim of the thesis was to explore a number of questions to assess the positive and negative effects of the WD, and to understand factors variations after setting a current knowledge of the effects of WD quality fruit. The questions were: 1) to characterize the effects of WD applied at specific stages of development of plant or fruit, 2) to understand the stress memory effect by alternating period of WD and recovery, 3) to test the stimulation of plant defences by WD during an infection by a pathogen, and 4) to study the genetic variability associated with responses to WD through the previous objectives. For this, several experiments were conducted in greenhouses and growth chambers. The parents of the MAGIC population of tomato (with rich allelic variability) were studied, as well as two commercial tomatoes (for the study of the interaction between WD and Botrytis cinerea). Plant response mechanisms induced during a WD were characterized in both plant vegetative and reproductive stages, and then during the fruit development stages (cell division, cell expansion and maturation). The plant vegetative and reproductive stages are distinguished by different mechanisms of oxidative stress management generated by WD. Vegetative plants are more affected at the photosystem II while reproductive plants rather modulate their water needs and responses at the photosystem I. The impact of WD on fruit quality differs according to fruit developmental stages. Strong genotypes effects were observed with an important increase in fruit taste quality (according to the concentrations in sugars and acids) for one genotype, which had poorer initial fruit quality than the second. Alternating periods of WD and recovery is a good compromise between water saving and plant adaptation (through proper management of oxidative stress). This alternation of stress has neutral to positive effects on fruit sugar content according to the genotypes and highly variable responses for contents of organic acids, carotenoids and ascorbic acid according to the genotypes. The interaction between a WD and B. cinerea generates a decrease of C/N ratio that can be associated to a stimulation of the pool of plant defence. However, the interaction between B. Cinerea and this WD promote pathogen development in stems. This deleterious effect of interaction seems attributable to hormonal regulation with the stimulation of the ABA pathway (by WD) and the SA pathway (by B. cinerea), which are antagonistic to the JA / ET pathway involved in response to necrotroph pathogens. However, a positive effect was observed for infections in detached leaves of infected plants, detached leaves represent a snapshot of plant defences and these results raise new questions. Overall, these data provide that WD improve fruit taste (sugars and acids) without yield losses but had variables effects on health promoting compounds. WD could permit the stimulation of plant defence but more research is needed. To finish, the genetic variability induced important variability in plant and fruit responses and interact with WD.

Total soluble solids (TSS) is a reliable indicator of melon eating quality, with a minimum standard of 10% recommended. The state of Australian melon production with respect to this quality criterion was considered within seasons, between growing districts and over seasons. It was concluded that improvement in agronomic practice and varietal selection is required to produce sweeter melons. The scientific literature addressing melon physiology and agronomy was summarised, as a background to the work that is required to improve melon production practices in Australia. The effect of source sink manipulation was assessed for commercially grown and glasshouse grown melon plants. The timing of fruit thinning, pollination scheduling, the application of a growth inhibitor and source biomass removal were assessed in relation to fruit growth and sugar accumulation. Results are interpreted against a model in which fruit rapidly increase in weight until about two weeks before harvest, with sugar accumulation continuing as fruit growth ceases. Thus treatment response is very dependant on timing of application. For example, fruit thinning at 25 days before harvest resulted in further fruit set and increased fruit weight but did not impact on fruit TSS (at 9.8%, control 9.3%), while thinning at 5 days before harvest resulted in a significant (Pless than 0.05) increase in fruit TSS (to 10.8%, control 9.3%) and no increase in fruit weight or number. A cost/ benefit analysis is presented, allowing an estimation of the increase in sale price required to sustain the implementation of fruit thinning. The effect of irrigation scheduling was also considered with respect to increasing melon yield and quality. To date, recommended practice has been to cause an irrigation deficit close to fruit harvest, with the intent of 'drying out' or 'stressing' the plant, to 'bring on' maturity and increase sugar accumulation. Irrigation trials showed that keeping plants stress-free close to harvest and during harvest, facilitated the production of sweeter fruit. The maintenance of a TSS grade standard using either batch based (destructive) sampling or (non-invasive) grading of individual fruit is discussed. On-line grading of individual fruit is possible using near infrared spectroscopy (NIR), but the applicability of the technique to melons has received little published attention. Tissue sampling strategy was optimised, in relation to the optical geometry used (in commercial operation in Australia), both in terms of the diameter and depth of sampled tissue. NIR calibration model performance was superior when based on the TSS of outer, rather than inner mesocarp tissue. However the linear relationship between outer and middle tissue TSS was strong (r2 = 0.8) in immature fruit, though less related in maturing fruit (r2 = 0.5). The effect of fruit storage (maturation/senescence) on calibration model performance was assessed. There was a negligible effect of fruit cold storage on calibration performance. Currently, the agronomist lacks a cost-effective tool to rapidly assess fruit TSS in the field. Design parameters for such a tool were established, and several optical front ends compared for rapid, though invasive, analysis. Further, for visualisation of the spatial distribution of tissue TSS within a melon fruit, a two-dimensional, or hyper-spectral NIR imaging system based on a low cost 8-bit charge coupled device (CCD) camera and filter arrangement, was designed and characterised.

Tesis por compendio / [ES] En las últimas dos décadas, la producción de caqui en España ha aumentado exponencialmente y la superficie de cultivo se ha multiplicado casi por ocho. En la actualidad, la producción de caqui está centralizada en el cultivar 'Rojo Brillante', que es mundialmente apreciado. Sin embargo, esta centralización supone una importante limitación comercial. En la presente Tesis se abordan dos objetivos principales: 1) Optimización de la tecnología pre y postcosecha con el fin de garantizar la calidad del caqui 'Rojo Brillante' durante la frigoconservación y el transporte a mercados internacionales. 2) Evaluación del comportamiento bajo condiciones mediterráneas de variedades introducidas de otras regiones productoras con el fin de ampliar el rango varietal de caqui. El primer objetivo de esta Tesis se logró a través de los estudios presentados en los Capítulos I al V. El segundo objetivo se abordó en los Capítulos VI y VII. En el Capítulo I se esclarecieron las causas del pardeamiento interno del fruto en los envíos a los mercados internacionales. La temperatura a la que se expone la fruta inmediatamente tras la aplicación del tratamiento de desastringencia con altas concentraciones de CO2 se identificó como el principal factor implicado en esta alteración. Los resultados obtenidos mostraron que esta alteración se puede evitar mediante la implementación de un período de atemperado de 24 h después del tratamiento con CO2, antes de conservar las frutas a bajas temperaturas. En el Capítulo II se evaluó la eficacia de un tratamiento de desastringencia recientemente patentado basado en la aplicación de una nueva cera que contiene etanol en su formulación. Los resultados mostraron que esta cera podría ser una alternativa al tratamiento convencional de desastringencia con altas concentraciones de CO2 cuando la fruta se envía a mercados lejanos a bajas temperaturas. La aplicación de la nueva cera antes de conservación en frío provocó la pérdida de astringencia de la fruta tras 30 días a 0 °C, preservando la firmeza y la calidad interna. Los resultados del Capítulo III demostraron que la realización de dos aplicaciones de ácido giberélico (AG) en precosecha permite retrasar el proceso de maduración de la fruta en el árbol con respecto a una sola aplicación. La combinación de AG en precosecha y 1-metilciclopropeno (1-MCP) en postcosecha resultó en una mayor calidad del fruto durante la frigoconservación en comparación con la aplicación únicamente de 1-MCP. Además, la fruta tratada con múltiples aplicaciones de AG mostró una firmeza ligeramente mayor tras de conservación a baja temperatura. En el Capítulo IV se demostró que, paralelamente al retraso de la maduración, el tratamiento con AG también retrasa la senescencia del cáliz, incrementando la calidad externa del fruto. La senescencia del cáliz durante la maduración del fruto se relacionó con una disminución de los parámetros de fluorescencia de clorofila (Fo, Fm y Fv/Fm). En el Capítulo V se estudiaron las condiciones óptimas de almacenamiento del caqui producido bajo cultivo ecológico. Según los resultados, no se deben almacenar los frutos cosechados con firmeza inferior a 30 N. La calidad del fruto durante la conservación dependió de las condiciones de almacenamiento, momento de aplicación del tratamiento de deastringencia y estado de madurez inicial. Por último, en los Capítulos VI y VII se abordó la evaluación bajo condiciones mediterráneas de 14 variedades de caquis no-astringentes procedentes de otros países. Se identificaron las variedades tempranas y tardías que pueden ser potencialmente interesantes para ampliar el período de cosecha en nuestra región. Los estudios postcosecha mostraron que todas las variedades estudiadas presentan una buena aptitud para ser comercializadas en los mercados nacionales y europeos. Sin embargo, la sensibilidad a los daños por frío que presentaron algunas de las variedades estudiadas podría limitar su comercialización a países de ultramar. Además, se observó que la respuesta al tratamiento con 1-MCP, para el control de los daños por frío, dependió en gran medida de la variedad. / [CA] En les últimes dues dècades, la producció de caqui a Espanya ha augmentat exponencialment i la superfície de cultiu s'ha multiplicat quasi per huit vegades. En l'actualitat, la producció de caqui està centralitzada en el la cultivar 'Rojo Brillante', que és mundialment apreciat. Malgrat això, aquesta centralització suposa d'una banda una important limitació comercial, ja que la producció es concentra en un període de collita relativament curt i, d'altra banda implica un alt risc fitosanitari. En la present Tesi s'aborden dos objectius principals: 1) Optimització de la tecnologia pre i postcollita amb la finalitat de garantir la qualitat del caqui 'Rojo brillante' durant la frigoconservació i el transport a mercats internacionals. 2) Avaluació del comportament de varietats introduïdes d'altres regions productores sota condicions mediterrànies amb la finalitat d'ampliar el rang varietal de caqui. En el Capítol I es van esclarir les causes de l l'enfosquiment intern. La temperatura a la qual s'exposa la fruita immediatament després de l'aplicació del tractament de desastringència amb altes concentracions de CO¿ es va identificar com el principal factor implicat en aquesta alteració. Els resultats obtinguts van mostrar que aquesta alteració es pot evitar mitjançant la implementació d'un període de temperat de 24 h després del tractament amb CO¿, abans de transferir la fruita a baixes temperatures. En el Capítol II es va avaluar l'eficàcia d'un tractament de desastringència recentment patentat basat en l'aplicació d'una nova cera que conté etanol en la seua formulació en caquis 'Rojo Brillante' i 'Triumph'. Els resultats van mostrar que aquesta cera podria ser una alternativa al tractament convencional de desastringència amb altes concentracions de CO¿ quan la fruita s'envia a mercats llunyans a baixes temperatures. L'aplicació de la nova cera abans de conservació en fred va provocar la pèrdua d'astringència de la fruita després de 30 dies a 0 °C, preservant la fermesa i la qualitat interna. Els resultats del Capítol III van demostrar que l'aplicació de dos tractaments d'àcid giberèlic (AG) en precollita permet retardar el procés de maduració de la fruita en l'arbre respecte a una sola aplicació. La combinació de AG en precollita i 1-metilciclopropeno (1-MCP) en postcollita va incrementar la qualitat del fruit durant la frigoconservació en comparació amb l'aplicació únicament de 1-MCP. A més, la fruita tractada amb múltiples aplicacions de AG va mostrar una fermesa lleugerament major després de conservació a baixa temperatura; no obstant això, una sola aplicació de AG també va ser eficaç per a previndre la manifestació de danys per fred en la fruita. En el Capítol IV es va demostrar que, paral·lelament al retard de la maduració, el tractament amb AG també retarda la senescència del calze, incrementant la qualitat externa del fruit. La senescència del calze durant la maduració del fruit es va relacionar amb una disminució dels paràmetres de fluorescència de clorofil·la (Fo, Fm i Fv/Fm). En el Capítol V es van estudiar les condicions òptimes d'emmagatzematge del caqui produït baix cultiu ecològic. D'acord amb els resultats obtinguts, no s'han d'emmagatzemar els fruits collits amb fermesa inferior a 30 N. La conservació del fruit va dependre de les condicions d'emmagatzematge, moment d'aplicació del tractament de desastringència i estat de maduresa de la fruita. Finalment, en els Capítols VI i VII es va abordar l'avaluació de 14 varietats de caquis no-astringents. Es van identificar les varietats primerenques i tardanes que poden ser potencialment interessants per a ampliar el període de collita. D'altra banda, els estudis postcollita van mostrar que totes les varietats estudiades presenten una bona aptitud per a ser comercialitzades en els mercats nacionals i europeus. No obstant això, la sensibilitat als danys per fred que van presentar algunes de les varietats estudiades podria limitar la seua comercialització a països d'ultramar. A més, es va observar que la resposta al tractament amb 1-MCP, per al control dels danys per fred, va dependre en gran manera de la varietat. / [EN] In the last two decades, persimmon production in Spain has increased exponentially and the cultivation area has expanded almost eight-fold. The current production of persimmon is focused on the cultivar 'Rojo Brillante', which is appreciated worldwide due to the high quality of the fruit. Nevertheless, centralized production of this single cultivar implies a major commercial limitation as its high volume of production is concentrated in a relative short harvesting period. Furthermore, it also implies a high phytosanitary risk. In this context, this Thesis addresses two main objectives: 1) Optimization of pre- and postharvest technology in order to guarantee the quality of 'Rojo Brillante' persimmon after cold storage and transportation to overseas markets. 2) Evaluation of the behavior of foreign varieties of persimmon under Mediterranean conditions in order to extend the varietal range. The first objective of this Thesis was tackled through the studies presented in Chapters I to V. The second objective was covered in Chapters VI and VII. Chapter I dealt with the causes of internal browning disorder manifested after cold shipment of 'Rojo Brillante' persimmons to overseas markets. The temperature to which fruit is exposed immediately after CO2 deastringency treatment was identified as the main factor involved in this alteration. Our results showed that this disorder can be avoided by implementing an attemperation period of 24 h after the CO2 treatment, before transferring fruit to cold storage. In Chapter II, the effectiveness of a recently patented deastringency treatment based on applying a new wax containing ethanol was assayed in 'Rojo Brillante' and 'Triumph' persimmons. The use of this new ethanol-based wax could be a potential alternative to the conventional CO2 deastringency treatment when the fruit are sent to distant overseas markets at low temperatures. Application of the new wax before cold storage led to loss of fruit astringency after 30 days at 0 °C while preserving fruit firmness and internal quality. The results of Chapter III demonstrated that two applications of gibberellic acid (GA) made it possible to delay the fruit maturity process on the tree more than with just a single application. The combination of GA and 1-methylciclopropene (1-MCP) preserved the fruit quality better than with the application of 1-MCP alone during cold storage. Moreover, the fruit treated by multiple GA applications showed a slightly higher firmness after cold storage. However, a single GA application was also effective in preventing the manifestation of chilling injury (CI) in fruit. Chapter IV demonstrated that in parallel to delaying maturity, the GA treatment also delayed calyx senescence, which meant that the fruit preserved a good appearance. The calyx lobe senescence during fruit ripening was linked to a decrease in all Chlorophyll Fluorescence Imaging (CFI) parameters (Fo, Fm, and Fv/Fm). Chapter V addressed the optimization of storage conditions for organic persimmons. According to our results, fruit harvested with a firmness lower than 30 N must not be stored. Fruit harvested with a higher level of firmness could be stored for up to three weeks depending on the storage conditions, the moment of application of the CO2 deastringency treatment, and the stage of fruit maturity. The longest storage period (3 weeks) was achieved when fruit were harvested with a firmness of around 45 N, submitted to CO2 treatment and then stored at 15 °C. Finally, 14 foreign varieties grown under Mediterranean conditions were evaluated to identify early- and late-season varieties with potential to extend the harvesting period. This is presented in Chapters VI and VII. In general, all the evaluated varieties fulfilled the quality criteria to be commercialized in domestic and European markets. However, sensitivity to CI was variety dependent and response to 1-MCP treatment was also observed to depend on the variety. / This study has been supported by the Instituto Nacional de Tecnología Agraria y Alimentaria de España (INIA) and Fondo Europeo de Desarrollo Regional (FEDER) through the projects RTA 2013-00043-C02-01 and RTA2017-00045-C02-01. In addition, it has been supported by the Valencian Institute for Agricultural Research (Project N° 51910). This study has also been founded by the Consellería de Educación, Investigación, Cultura y Deporte de la Comunidad Valenciana (project GV/2016/183). Ayoub Fathi Najafabadi thanks the INIA for grant FPI-INIA #43 (CPD2015-0151). The authors thank Fomesa Fruitech S.L.U. and Natural Hand S. L. for their technical support, Cooperativa Agrícola Ntra Sra de Loreto for its technical support and for making the plots and fruit used in this study available and Plant Production Department (Instituto Valenciano de Investigaciones Agrarias) for providing the fruit weight data and the photographs of the different varieties. / Fathi Najafabadi, A. (2021). Optimization of postharvest Technology for "Rojo brillante" Persimmon and Introduction of New Varieties in the Mediterranean Area [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/171252 / TESIS / Compendio