This study consisted of three parts. We first used a video observation method and statistical analysis to identify benign behaviors that occurred before the problem behavior. These benign behaviors are known as precursor behaviors. We then used a precursor functional analysis to identify the function of the precursor behaviors. Lastly, we developed and implemented an intervention based on the results of the precursor functional analysis. The interventions effectively reduced problem behavior for the participants in the study. The participants engaged in minimal instances of problem behavior throughout the study. The results of this study may be useful to teachers and caregivers of children who engage in severe problem behavior that would be unsafe to reinforce in an assessment. Results may also help when working with children whose caregivers are concerned with their children engaging in excessive problem behavior. Further research is needed to look at modifying the precursor analysis. Future research should also look at the social validity of the precursor assessment.

Magister Artium (Psychology) - MA(Psych) / The purpose of the present study was to gather further external validity evidence towards the validity argument for an instrument designed to measure individual and contextual factors associated with adolescent substance use in low socio-economic status communities in the Western Cape, South Africa. The South African Substance Use Contextual Risk Instrument (SASUCRI) measures adolescents’ subjective experiences of their own psycho-social and their communities’ functioning. The present study uses secondary data analysis in order to further evaluate its external validity. Both content and structural evidence for the instrument has been gathered in the larger study in which the present study is located. Validity theory was used as the theoretical framework for the gathering of the different types of evidence in support of the validity argument for this instrument. The study employed non-probability purposive sampling to select schools from three education districts from which twenty-six schools were selected where the sample total was N=1959. English and Afrikaans versions of the instrument were administered to English- and Afrikaans home language, school-going adolescents, aged 12 to 21 years. All ethical standards were maintained throughout the research process. External evidence procedures were conducted using Discriminant Function Analysis (DFA) to evaluate the extent to which the instrument could discriminate between substance using and non-using adolescents. The DFA revealed that nine SASUCRI sub-scales totals can act as significant predictors to substance use among adolescents based on the predictive validity of sub-scales.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第21425号 / 工博第4535号 / 京都大学大学院工学研究科合成・生物化学専攻 / (主査)教授 浜地 格, 教授 杉野目 道紀, 教授 秋吉 一成 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

Made available in DSpace on 2016-04-04T18:27:16Z (GMT). No. of bitstreams: 1 ana claudia.pdf: 454849 bytes, checksum: 2ec7642700236761ec5d3bf8ff3aebc8 (MD5) Previous issue date: 2006-02-08 / Pontif?cia Universidade Cat?lica de Campinas / Cancer is a disease characterized by the loss of the control of the cellular division and by the capacity of these disorganized cells to invade others organic structures. In Brazilian women, the breast cancer is the type of cancer that causes more deaths, but if it is detected early, there are large chances of cure. Many times it is discovered late which leads to mutilation treatment to the woman, as surgical removal of one or both breasts, partially or completely (mastectomy) followed by chemotherapy and/or radiotherapy. Undergoing a mastectomy because of a chronic disease has a very complex connotation to the women because their symbol of femininity, seduction and maternity has been removed. The changes in these women lives are in the personal, familiar and professional field. Therefore, it is extremely important that researches are made, seeking to know about the behaviors that contribute to the promotion of the psychological well-being of these women. This research had for objective to accomplish a functional analysis of the women's behaviors after the mastectomy surgery. Were interviewed four women that went by the mastectomy, with ages varying among 34 and 49 years old, leaded by a mastologist. The interviews were recorded and completely transcribed. The women also maintained a written registration, during fifteen days, in which they specified the context, the actions, feelings and thoughts corresponding to the situation, as well as their consequences of the existences related to the cancer. Categories of behaviors were identified and analyzed in their functions. The categories more mentioned by that sample were: believes (faith and faith in God and behaviors optimists), received social and family support and reflections regarding the own life. For each category of behavior, a functional analysis was accomplished as well as some were given examples of the participants verbalizations that demonstrate the antecedents, the behaviors and the consequences of these. It was verified that having faith and believing in God were behaviors that evoke positive consequences that can contribute to face the cancer. Optimistic behaviors increase the occurrence probability of the adhesion behaviors to the treatment (as taking medications, to going to the doctor, for instance). In the participants verbalization, we can observe that the social and familiar support is considered extremely important to the women. The context which women with breast cancer are involved also provides reflections about their own lives, contributing to self-knowledge. The diagnosis of the cancer, its treatment and its consequences are considered antecedent stimulus that evoke the behaviors included by the mentioned categories. Those behaviors generate diversified consequences, as positive and negative reinforcement. With this procedure it was possible to know some variables that act in the post-mastectomy atmosphere, which contributes to the elaboration of analyticalbehavior intervention programs that could benefit women with breast cancer. / O c?ncer ? uma doen?a que se caracteriza pela perda do controle da divis?o celular e pela capacidade destas c?lulas desorganizadas invadirem outras estruturas org?nicas. Nas mulheres brasileiras, o c?ncer de mama ? o tipo de neoplasia que mais causa mortes, por?m se detectado precocemente, h? alta chances de cura. Muitas vezes, ele ? descoberto tardiamente, gerando tratamento mutilantes ? mulher, como a retirada parcial ou total da mama e a realiza??o da quimioterapia e/ou radioterapia. Extirpar a mama em decorr?ncia de uma enfermidade cr?nica possui uma conota??o extremamente complexa para a mulher, pois o s?mbolo de feminilidade, sedu??o e maternidade foi amputado. As mudan?as na vida desta mulher englobam as esferas pessoal, profissional e familiar. Assim, ? de extrema import?ncia que pesquisas sejam feitas, visando conhecer os comportamentos que contribuem com a promo??o do bem-estar psicol?gico. Para tanto, esta pesquisa teve por objetivo realizar uma an?lise funcional dos comportamentos de mulheres ap?s a cirurgia de mastectomia. Foram entrevistadas quatro mulheres que passaram pela mastectomia, com idades variando de 34 a 49 anos, encaminhadas por um mastologista. As entrevistas foram gravadas e transcritas em sua ?ntegra. As mulheres mantiveram tamb?m um registro escrito, durante quinze dias, em que especificaram o contexto, as a??es, sentimentos e pensamentos correspondentes ? situa??o, bem como suas conseq??ncias, das viv?ncias relacionadas ao c?ncer. Categorias de comportamentos foram identificadas e analisadas em suas fun??es. As categorias mais citadas por essa amostra foram: cren?as (m?sticas e otimistas), receber apoio social e familiar e busca pelo auto-conheimento. Para cada categoria de comportamento foi realizada uma an?lise funcional bem como foram dados alguns exemplos de verbaliza??es das participantes que demonstram os antecedentes, os comportamentos e as conseq??ncias destes. Verificou-se que acreditar em um ser superior que possa trazer a cura s?o comportamentos que desencadeiam conseq??ncias positivas que podem contribuir para o enfrentamento da enfermidade. Os comportamentos otimistas aumentam a probabilidade de ocorr?ncia dos comportamentos de ades?o ao tratamento (como tomar medica??es, ir ao m?dico, por exemplo). Nas verbaliza??es das participantes observa-se que o apoio social e familiar recebido ? considerado de extrema import?ncia no contexto vivenciado. O contexto ao qual encontram-se as mulheres com c?ncer de mama tamb?m proporcionaram reflex?es ? respeito da pr?pria vida, contribuindo com o autoconhecimento. O diagn?stico do c?ncer, seu tratamento e suas conseq??ncias s?o considerados est?mulos antecedentes, que evocam os comportamentos englobados pelas categorias citadas. Esses comportamentos, por sua vez, geram conseq??ncias diversificadas, como refor?o positivo e negativo. Desta maneira, com esse procedimento foi poss?vel conhecer algumas vari?veis que atuam no ambiente p?s-mastectomia, o que contribui para elabora??o de programas de interven??o anal?tico-comportamentais que poderiam beneficiar as mulheres acometidas por c?ncer de mama

Made available in DSpace on 2014-06-11T19:28:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-24Bitstream added on 2014-06-13T18:58:15Z : No. of bitstreams: 1 oliveira_j_me_bauru.pdf: 823647 bytes, checksum: aa43b296bcb60ebe028233da39c36d84 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A literatura em Análise do Comportamento tem demonstrado aumento nos estudos sobre procedimentos metodológicos para o ensino de parte dos repertórios que definem a avaliação funcional para não-especialistas. O objetivo deste trabalho foi verificar se as condições metodológicas delineadas poderiam favorecer o ensino de repertórios de interpretação funcional para uma professora (P) da 3ª série do Ensino Fundamental para conteúdos curriculares do ensino de Ciências Naturais, bem como, averiguar possíveis alterações em suas práticas de ensino em sala de aula que poderiam estar relacionadas às atividades executadas neste estudo. O procedimento foi dividido em 3 etapas. Na Etapa 1 foi realizada uma entrevista inicial de rapport para conhecer a descrição de P sobre a turma. Em seguida foram gravados dois conjuntos de aulas lecionadas por P sobre sobre temas distintos e consecutivos (UDI e UD2. Ao final desta etapa, P foi submetida a um Roteiro de Entrevistas que continha questões acerca de suas próprias aulas. O Roteiro foi aplicado na ausência e na presença dos episódios selecionados da UD1. Durante a Etapa 2, P foi exposta aos modelos de interpretação funcional elaborados pelo pesquisador sobre os mesmos episódios selecionados da UD1, por conseguinte, a professora foi solicitada a executar suas próprias tentativas de interpretações funcionais para os episódios da UD2. A Etapa 2 foi finalizada com a exposição para P dos modelos de interpretação funcional elaborados pelo pesquisador para os episódios da UD2. Na Etapa 3 ocorreu a gravação de um novo conjunto de aulas (UD3). Nesta etapa foram editados novos episódios da UD3. Tais episódios foram exibidos a P conjuntamente ao Roteiro de Entrevistas utilizado nas etapas anteriores, ao final de cada episódio o roteiro foi reapresentado. Por fim foi solicitado... / The literature on behavior analysis has shown an increase in studies on methodological procedures for teaching the repertories that define the functional assessment for non-specialists. The goal of this study was to verify whether the outline methodological conditions could favor the functional interpretation repertoire teaching for a teacher (P) in the 3rd grade of elementary school for curriculum content of natural sciences discipline, as well as verify whether possible changes in their classroom teaching practices could be connected to activities performed in this study. The experiment had three steps. In step 1 was held an initial rapport interview to know P's description of her class. Then it were recorded two sets of lessons (UD1 and UD2) taught by P about distinct and consecutive themes. At the end of this step, P was submitted to n interview script with questions about her classes. The interview script was applied both in the absence or presence of UD1's selected episodes. During Stage 2, P was exposed to functional interpretation models developed by the researches onn the same UD1's selected episodes, then the teacher was asked to run her own functional interpretation of UD2 episodes. Step 2 ended with the exposure for P of the functional interpretation models developedby the researcher for UD2 episodes. In Step 3 a new set of classes (UD3) were recorded. In this step new episodes were then shown to P along with the interview scripts from previous steps, and at the end of each episode the script was presented again. Finally P was asked to compare UD2 and UD3. P reports about her practices proved to be different than functional interpretation proposal, forgone possible control relations between the operating properties of the provided teaching conditions and students performance in light of such practices. These characteristics were... (Complete abstract click electronic access below)

Orientador: Jair Lopes Júnior / Banca: João dos Santos Carmo / Banca: Paulo Sergio Teixeira do Prado / Resumo: A literatura em Análise do Comportamento tem demonstrado aumento nos estudos sobre procedimentos metodológicos para o ensino de parte dos repertórios que definem a avaliação funcional para não-especialistas. O objetivo deste trabalho foi verificar se as condições metodológicas delineadas poderiam favorecer o ensino de repertórios de interpretação funcional para uma professora (P) da 3ª série do Ensino Fundamental para conteúdos curriculares do ensino de Ciências Naturais, bem como, averiguar possíveis alterações em suas práticas de ensino em sala de aula que poderiam estar relacionadas às atividades executadas neste estudo. O procedimento foi dividido em 3 etapas. Na Etapa 1 foi realizada uma entrevista inicial de "rapport" para conhecer a descrição de P sobre a turma. Em seguida foram gravados dois conjuntos de aulas lecionadas por P sobre sobre temas distintos e consecutivos (UDI e UD2. Ao final desta etapa, P foi submetida a um Roteiro de Entrevistas que continha questões acerca de suas próprias aulas. O Roteiro foi aplicado na ausência e na presença dos episódios selecionados da UD1. Durante a Etapa 2, P foi exposta aos modelos de interpretação funcional elaborados pelo pesquisador sobre os mesmos episódios selecionados da UD1, por conseguinte, a professora foi solicitada a executar suas próprias tentativas de interpretações funcionais para os episódios da UD2. A Etapa 2 foi finalizada com a exposição para P dos modelos de interpretação funcional elaborados pelo pesquisador para os episódios da UD2. Na Etapa 3 ocorreu a gravação de um novo conjunto de aulas (UD3). Nesta etapa foram editados novos episódios da UD3. Tais episódios foram exibidos a P conjuntamente ao Roteiro de Entrevistas utilizado nas etapas anteriores, ao final de cada episódio o roteiro foi reapresentado. Por fim foi solicitado... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The literature on behavior analysis has shown an increase in studies on methodological procedures for teaching the repertories that define the functional assessment for non-specialists. The goal of this study was to verify whether the outline methodological conditions could favor the functional interpretation repertoire teaching for a teacher (P) in the 3rd grade of elementary school for curriculum content of natural sciences discipline, as well as verify whether possible changes in their classroom teaching practices could be connected to activities performed in this study. The experiment had three steps. In step 1 was held an initial rapport interview to know P's description of her class. Then it were recorded two sets of lessons (UD1 and UD2) taught by P about distinct and consecutive themes. At the end of this step, P was submitted to n interview script with questions about her classes. The interview script was applied both in the absence or presence of UD1's selected episodes. During Stage 2, P was exposed to functional interpretation models developed by the researches onn the same UD1's selected episodes, then the teacher was asked to run her own functional interpretation of UD2 episodes. Step 2 ended with the exposure for P of the functional interpretation models developedby the researcher for UD2 episodes. In Step 3 a new set of classes (UD3) were recorded. In this step new episodes were then shown to P along with the interview scripts from previous steps, and at the end of each episode the script was presented again. Finally P was asked to compare UD2 and UD3. P reports about her practices proved to be different than functional interpretation proposal, forgone possible control relations between the operating properties of the provided teaching conditions and students performance in light of such practices. These characteristics were... (Complete abstract click electronic access below) / Mestre

Submitted by Erika Demachki (erikademachki@gmail.com) on 2015-05-22T16:58:51Z No. of bitstreams: 2 Dissertação - Jorge Hernan Garcia Homez - 2014.pdf: 6822156 bytes, checksum: ee54639ad53f9e91bb703af1cb86d568 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2015-05-22T18:14:52Z (GMT) No. of bitstreams: 2 Dissertação - Jorge Hernan Garcia Homez - 2014.pdf: 6822156 bytes, checksum: ee54639ad53f9e91bb703af1cb86d568 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2015-05-22T18:14:52Z (GMT). No. of bitstreams: 2 Dissertação - Jorge Hernan Garcia Homez - 2014.pdf: 6822156 bytes, checksum: ee54639ad53f9e91bb703af1cb86d568 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-08-27 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Studies developed by the state road agency (AGETOP) in 2010, regarding the road infrastructure in the state of Goiás, indicated a need for rebuild 60% of the road network to reestablish its service level. To this end, in 2011, was create the program of road restoration "Rodovida", which aims to restore road infrastructure in the state along four years. In order to obtain parameters for evaluation of performed services, was signed an agreement between the government inspection agency (GO-CGE) and the federal university (UFG). This investigation was developed with the goal to made structural and functional analysis of restored pavements using full depth reclamation technique with gravel addition, to evaluate if the solutions defined in the projects enable the restored pavements meet, or not, the technical parameters of the current road standards, and therefore provide parameters for the incorporation of these reconstruction techniques in future projects. For this study were select three roads with pavements reconstruction in progress, in which procedures for recycling and compacting base monitored by sand bottle test, Speedy Moisture Test and the Dynamic Cone Penetrometer (DCP). Also deformed samples of recycled material for laboratory testing were collect, aiming to propose a model that relates California Bearing Ratio (CBR) with the Penetration Index (DN) for bases added crushed gravel, obtained from the analysis with the DCP in compacted specimens. For structural analysis, were performed field DCP tests to determine the resistance to dynamic penetration and stratigraphic evaluation. As well as testing with Benkelman beam for determining deflections. With the stratigraphy data collected from DCP and displacement basins with the Benkelman beam were determined resilience modulus through back analysis with the aid of the KENLAYER iterative tool. The results obtained with DCP showed thickness variation in the recycled layers, showing the need for greater control during restoration process. Respect to the penetration index determined for the tested bases, these values had increased variability that may be associated with loss control in the executive process, now for sub-base and subgrade layers were determined heterogeneous values, concluding that although high strength present in the recycled bases, this resistance, as well as the thickness of the bases, is heterogeneous. In terms of deflection, the three studied roads showed values within a satisfactory variation range, although some parts were identify with large values of deflection. In terms of back calculated modules, the obtained results can be considered heterogeneous, which may be associated to the recycling technique used or the fact that were adopt different restoration solutions along the evaluated section on a highway. As such, is concluded that even being adopted similar recycling solutions in the three studied roads, the analysis shows distinct behaviors connected mainly to executive processes used. / Estudos desenvolvidos pela Agência Goiana de Transportes e Obras (AGETOP) a respeito da infraestrutura rodoviária do estado de Goiás, no ano 2010, indicaram a necessidade de reconstrução de 60% para recompor seu nível de serviço. Para tanto, em 2011, foi criado o programa de restauração rodoviário “Rodovida”, o qual visa recuperar a infraestrutura viária do estado ao longo de quatro anos. Com a finalidade de obter parâmetros para avaliação dos serviços executados, foi firmado um convênio entre a Controladoria Geral do Estado de Goiás (CGE-GO) e a Universidade Federal de Goiás (UFG). Esta dissertação foi desenvolvida com o objetivo de realizar análises estruturais e funcionais de rodovias restauradas com emprego da técnica de reciclagem profunda a frio “in situ” e incorporação de brita, visando avaliar se as soluções definidas nos projetos possibilitam que os pavimentos restaurados atendam, ou não, parâmetros técnicos das normas rodoviárias atuais, e assim fornecer parâmetros para a incorporação dessas técnicas de reconstrução em futuros projetos. Para tanto foram selecionadas três obras de reconstrução de pavimentos em andamento, nas quais foram monitorados os procedimentos de reciclagem e compactação de base através de ensaios de frasco de areia, “Speedy Moisture Test” e o Penetrômetro Dinâmico de Cone (DCP). Também foram coletadas amostras deformadas do material reciclado para ensaios de laboratório, visando propor um modelo que relacione o Índice de Suporte California (ISC) com o Índice de Penetração (DN) para bases de cascalho com acréscimo de brita, obtido a partir do ensaio com o DCP em corpos de prova compactados. Para a análise estrutural, foram realizados ensaios de DCP em campo para determinação da resistência à penetração dinâmica e avaliação estratigráfica, assim como ensaios com a viga Benkelman, para a determinação das bacias de deflexões. Com os dados de estratigrafia coletados com o DCP e as bacias de deslocamento com a viga Benkelman, foram determinados os módulos de resiliência por meio de retroanálise interativa com auxílio do programa KENLAYER. Os resultados obtidos com o DCP mostraram a variação nas espessuras das camadas recicladas, gerando a necessidade de realizar um maior controle durante a execução. Com relação ao índice de penetração determinado para as bases ensaiadas, esses valores apresentaram maior variabilidade que pode estar associada à falta de controle no processo executivo; já nas camadas de sub-base e subleito foram determinados valores heterogêneos, concluindo que embora as bases recicladas apresentem elevada resistência, essa resistência, assim como a espessura das bases, é heterogênea. Em termos de deflexão, as três rodovias apresentaram valores dentro de uma faixa de variação considerada satisfatória, apesar de serem identificadas algumas regiões com elevados valores de deflexão. Em termos de módulos retroanalisados, os resultados obtidos podem ser considerados heterogêneos, o qual pode ser associado ou com a técnica de reciclagem utilizada ou ao fato de terem sido adotadas diferentes soluções de restauração ao longo do trecho avaliado numa rodovia. Sendo assim, conclui-se que mesmo sendo adotadas soluções de reciclagem semelhantes nas três rodovias estudadas, as análises realizadas mostram comportamentos distintos vinculados, principalmente, a processos executivos utilizados.

Le programme d’élimination du paludisme de l’OMS est menacé par l’émergence etla propagation potentielle de parasites de l’espèce Plasmodium falciparum résistants à l’artémisinine. Récemment il a été montré que (a) des SNPs dans une région du chromosome 13 subissaient une forte sélection positive récente au Cambodge,(b) plusieurs sous-populations de parasites de P. falciparum résistants et sensibles à l’artémisinine étaient présentes au Cambodge, (c) des mutations dans le domaine Kelch du gène k13 sont des déterminants majeurs de la résistance à l’artémisinine dans la population parasitaire cambodgien et (d) des parasites de sous-populations du nord du Cambodge près de la Thaïlande et du Laos sont résistants à la méfloquine et portent l’allèle R539T du gène de k13.Il est donc nécessaire d’identifier la base génétique de la résistance dans le but de surveiller et de contrôler la transmission de parasites résistants au reste du monde, pour comprendre le métabolisme des parasites et pour le développement de nouveaux médicaments. Ce travail a porté sur la caractérisation de la structure de la population de P. falciparum au Cambodge et la description des propriétés métaboliques des sous-populations présentes ainsi que des flux de gènes entre ces sous-populations. Le but est d’identifier les bases génétiques associées à la transmission et l’acquisition de résistance à l’artémisinine dans le pays.La première approche par code-barre a été développée pour identifier des sous-populations à l’aide d’un petit nombre de loci. Une approche moléculaire de PCR-LDR-FMA multiplexée et basée sur la technologie LUMINEX a été mise au point pour identifier les SNP dans 537 échantillons de sang (2010 - 2011) provenant de 16centres de santé au Cambodge. La présence de sous-populations le long des frontières du pays a été établie grâce à l’analyse de 282 échantillons. Les flux de gènes ont été décrits à partir des 11 loci du code-barre. Le code-barre permet d’identifier les sous-populations de parasites associées à la résistance à l’artémisinine et à la méfloquine qui ont émergé récemment.La seconde approche de caractérisation de la structure de la population de P.falciparum au Cambodge a été définie sur la base de l’analyse de 167 génomes de parasites (données NGS de 2008 à 2011) provenant de quatre localités au Cambodge et récupérés à partir de la base de données ENA. Huit sous-populations de parasites ont pu être décrites à partir d’un jeu de 21257 SNPs caractérisés dans cette étude. La présence de sous-populations mixtes de parasite apparait comme un risque majeur pour la transmission de la résistance à l’artémisinine. L’analyse fonctionnelle montre qu’il existe un fond génétique commun aux isolats dans les populations résistantes et a confirmé l’importance de la voie PI3K dans l’acquisition de la résistance en aidant le parasite à rester sous forme de stade anneau.Nos résultats remettent en question l’origine et la persistance des sous-populations de P. falciparum au Cambodge, fournissent des preuves de flux génétique entre les sous-populations et décrivent un modèle d’acquisition de résistance à l’artémisinine.Le processus d’identification des SNPs fiables a été ensuite appliqué au génome de Babesia microti. Ce parasite est responsable de la babésiose humain (un syndrome de type malaria) et est endémique dans le nord-est des Etats-Unis. L’objectif était de valider la position taxonomique de B. microti en tant que groupe externe aux piroplasmes et d’améliorer l’annotation fonctionnelle du génome en incluant la variabilité génétique, l’expression des gènes et la capacité antigénique des protéines. Nous avons ainsi identifié de nouvelles protéines impliquées dans les interactions hôte-parasite. / The undergoing WHO Malaria elimination program is threatened by the emergenceand potential spread of the Plasmodium falciparum artemisinin resistant parasite.Recent reports have shown (a) SNPs in region of chromosome 13 to be understrong recent positive selection in Cambodia, (b) presence of P. falciparum parasiteresistant and sensitive subpopulations in Cambodia, (c) the evidence that mutationsin the Kelch propeller domain of the k13 gene are major determinants ofartemisinin resistance in Cambodian parasite population and (d) parasite subpopulations in Northern Cambodia near Thailand and Laos with mefloquine drugresistance and carrying R539T allele of the k13 gene.Identifying the genetic basis of resistance is important to monitor and control thetransmission of resistant parasites and to understand parasite metabolism for the development of new drugs. This thesis focuses on analysis of P. falciparum population structure in Cambodia and description of metabolic properties of these subpopulations and gene flow among them. This could help in identifying the genetic evidence associated to transmission and acquisition of artemisinin resistance over the country.First, a barcode approach was used to identify parasite subpopulations using smallnumber of loci. A mid-throughput PCR-LDR-FMA approach based on LUMINEXtechnology was used to screen for SNPs in 537 blood samples (2010 - 2011) from 16health centres in Cambodia. Based on successful typing of 282 samples, subpopulations were characterized along the borders of the country. Gene flow was described based on the gradient of alleles at the 11 loci in the barcode. The barcode successfully identifies recently emerging parasite subpopulations associated to artemisinin and mefloquine resistance.In the second approach, the parasite population structure was defined based on167 parasite NGS genomes (2008 - 2011) originating from four locations in Cambodia,recovered from the ENA database. Based on calling of 21257 SNPs, eight parasite subpopulations were described. Presence of admixture parasite subpopulation couldbe supporting artemisinin resistance transmission. Functional analysis based on significant genes validated similar background for resistant isolates and revealed PI3K pathway in resistant populations supporting acquisition of resistance by assisting the parasite in ring stage form.Our findings question the origin and the persistence of the P. falciparum subpopulations in Cambodia, provide evidence of gene flow among subpopulations anddescribe a model of artemisinin resistance acquisition.The variant calling approach was also implemented on the Babesia microti genome.This is a malaria like syndrome, and is endemic in the North-Eastern USA. Theobjective was to validate the taxonomic position of B. microti as out-group amongpiroplasmida and improve the functional genome annotation based on genetic variation, gene expression and protein antigenicity. We identified new proteins involved in parasite host interactions.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第18303号 / 工博第3895号 / 新制||工||1598(附属図書館) / 31161 / 京都大学大学院工学研究科合成・生物化学専攻 / (主査)教授 濵地 格, 教授 森 泰生, 教授 跡見 晴幸 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

Made available in DSpace on 2016-07-27T14:21:19Z (GMT). No. of bitstreams: 1 DAISY CLEIA OLIVEIRA DOS SANTOS.pdf: 558943 bytes, checksum: 70bada0301e7f7b0f1140b3df01ae600 (MD5) Previous issue date: 2007-03-09 / Since the initial definition of schizophrenia, the psychotic speech has been a constant goal of analysis. In this study, it was investigated the relation between three different interventions on valuation of the verbal behavior of a person who had been diagnosed with schizophrenia. For all the interventions it has established the social reinforcement to the appropriated speech. Besides a procedure of interruption of the social attention for the psychotic speech in the Intervention 1, the functional analysis of the contents of the psychotic speech in the Intervention 2, and a verbal skills training in the Intervention 3. The program of intervention was effective on the diminution of the psychotic speech and on the amplitude of the appropriated speech. With principal effects in the Interventions 2 and 3. In consequence, the psychotic speech shows modifications according on the programmed contingences. / Desde a definição inicial da esquizofrenia, a fala psicótica tem sido alvo constante de análise. Neste estudo investigou-se a relação entre três diferentes intervenções na avaliação do comportamento verbal de uma pessoa com o diagnóstico de esquizofrenia. Em todas as intervenções, estabeleceu-se o reforçamento social para as falas apropriadas. Além de um procedimento de suspensão da atenção social para as falas psicóticas na Intervenção 1, a análise funcional dos conteúdos da fala psicótica na Intervenção 2, e um treinamento de habilidade verbal na Intervenção 3. O programa de intervenção foi efetivo na diminuição das falas psicóticas e na ampliação das falas apropriadas. Com modificações comportamentais significativas nas Intervenções 2 e 3. Conseqüentemente, a fala psicótica mostrou-se modificável de acordo com as contingências programadas.