Large-scale land uses such as residential wastewater discharge and coal mining practices, particularly surface coal extraction and associated valley fills, are of particular ecological concern in central Appalachia. Identification and quantification of both large-scale land use and microhabitat alterations to ecosystems are a necessary first-step aid in mitigation of negative consequences to biota. In central Appalachian headwater streams absent of fish, salamanders are the dominant, most abundant vertebrate predator providing a significant intermediate trophic role. Stream salamander species are considered to be sensitive to aquatic stressors and environmental alterations with past research having shown linkages among microhabitat parameters, and large-scale land use such as urbanization and logging to salamander abundances. However, there is little information examining these linkages in the coalfields of central Appalachia. In the summer of 2013, I visited 70 sites (sampled three times each) in the southwest Virginia coalfields to collect salamanders and quantify stream and riparian microhabitat parameters. In an information-theoretic framework I compared the effects of microhabitat and large-scale land use on salamander occupancy and abundances. My findings indicate that dusky salamander (Desmognathus spp.) occupancy and abundances are more correlated to microhabitat parameters such as canopy cover than to subwatershed land uses. Brook salamander (Eurycea spp.) occupancy show negative associations to large-scale land uses such as percent recent mining and percent forested. Whereas Eurycea spp. abundances are negatively influenced by suspended sediments, stream bank erosion and stream substrate embeddedness. Management implications of these findings include erosion prevention and control as well as protection and management of riparian habitats. However, quantifying physical environmental quality such as stream and riparian habitat often can be quite difficult, particularly when there are time or fiscal limitations. In order to accurately assess stream and riparian habitat in a time- and cost- effective manner, the U.S. Army Corps of Engineers (USACE) developed a functional condition index (FCI) assessment for streams that measures 11 stream and riparian parameters along with watershed land use to calculate three different scores: a hydrology score, biogeochemical score, and habitat score (Noble et al 2010). Using the salamander data from 2013, I then analyzed the FCI scores using collected occupancy and abundance analyses. Both analyses supported the Habitat FCI score as it had strong correlations with both occupancy and abundance of three Desmognathus spp., and support the use of the USACE protocol for stream and riparian habitat assessment. / Ph. D.

Prior studies have demonstrated Appalachian coal mining often causes elevated specific conductance (SC) in streams, and others have examined SC effects on benthic macroinvertebrate communities using point-in-time SC measurements. However, both SC and benthic macroinvertebrate communities exhibit temporal variation. Twelve Appalachian headwater streams with minimally impacted physical habitat and reference-quality physicochemical conditions (except elevated SC) were sampled ten to fourteen times each for benthic macroinvertebrates between June 2011 and November 2012. In situ loggers recorded SC at 15-minute intervals. Streams were classified by mean SC Level (Reference 17-142 S/cm, Medium 262-648 S/cm, and High 756-1,535 S/cm). Benthic macroinvertebrate community structure was quantified by the Virginia Stream Condition Index and other metrics. Structural metric differences among SC Levels and month of sampling were explored. Reference-SC streams exhibited significantly higher scores on most metrics, supporting previous findings that SC may act as a biotic stressor, even in streams lacking limitations from degradation of physical habitat or other physicochemical conditions. Temporal variation was greatest in Medium-SC streams, which had the most metrics exhibiting significant differences among months and the greatest range of monthly means for six metrics. Metrics involving % Plecoptera and/or % Trichoptera were not sensitive to elevated SC, as Leuctridae and Hydropsychidae exhibited increased abundance in streams with elevated SC. Best scores for benthic macroinvertebrate community metrics differed based on selected metric, SC Level, and month. Consequently, timing of sampling is important, particularly in streams with elevated SC because community metric scores may be impacted by dominant taxa life history patterns. / Master of Science

One of the primary concerns associated with timber harvesting is the production of sediments from stream crossings. While research has shown that using improved haul road crossings can mitigate sediment production in perennial streams compared to the use of unimproved crossings little research has been undertaken on temporary skidder crossings of headwater streams, a situation common to a significant percentage of ground skidding operations. This experiment consisted of a controlled replicated testing of the effectiveness of four types of temporary skidder stream crossings (unimproved ford, corrugated culvert, wood panel skidder bridge, and PVC pipe bundle) relative to bedload and suspended sediment production. Automated samplers were used to monitor sediment and bedload production during the construction, use, removal, and post-removal phases associated with the use of these temporary crossings. Results showed that elevated crossings mitigated total sediment production compared to unimproved fords. Further, wood panel bridges yielded lower amounts of sediment than culverts but PVC pipe bundles show no difference between bridges or culverts. Sediment production varied by crossing type and use phase. While no differences were found among crossings types during construction, there was a difference between improved crossings and fords during use. Further, bridges and PVC pipe bundle crossings produced significantly less sediments than culverts during both their removal and during post-removal sampling and fords produced the largest amount of sediments during these phases.

Inland waters are important systems for transforming, storing and transporting carbon along the aquatic continuum, but also by emitting carbon dioxide (CO2) and methane (CH4) to the atmosphere. In light of the last decades observed increase in dissolved organic carbon (DOC) in many inland waters across the northern hemisphere, a logical question arise whether other aquatic carbon species display similar trends. This study examined the measured concentrations of dissolved inorganic carbon (DIC) in a boreal catchment over a 14-year period. The objectives were to determine changes in DIC concentration over time and try to explain the causes for the observed changes. Data from 15 mostly forested sub-catchments were analyzed, both over the full time period, and grouped by season. Over the full 14-year period, only two of the sites exhibited significant trends in DIC concentration, both being negative. However, by seasonally grouping the data distinct patterns for the different seasons emerged. The autumn and winter data displayed no significant trends, whereas the spring flood data showed significant negative trends for almost all sites (14 out of 15). The summer data showed significant negative trends for seven sites, and positive for one site. The DIC concentration data were expectedly positively correlated with pH across most sites (13 out of 15). The correlation with DOC was negative for most sites (11 out of 15), possibly indicating different origins of the different carbon species. The DIC concentration was also negatively correlated with discharge for most sites (13 out of 15), suggesting a diluting effect with increased discharge. In conclusion, significant negative trends were observed during the spring flood and summer periods. Although the cause of these trends will require further investigation, the correlation analysis showed that the DIC concentration was closely related to the catchment hydrology. This suggests changes in terrestrial source areas where DIC is mobilized during spring and summer, and that these changes might continue during altered hydrometeorological conditions. The differences in DIC trends between sub-catchments further show the variability of the boreal landscape and highlight the need for local-scale process understanding when scaling to larger landscape units. We further conclude that trends in DIC concentration do not follow observed DOC changes over time, suggesting that DIC and DOC exports are mechanistically decoupled.

Master of Arts / Department of Geography / Melinda Daniels / Large woody debris, (LWD), defined as pieces measuring ≥ 1 meter in length and ≥ 10 centimeters in diameter (Swanson and Lienkaemper, 1978; Marston, 1982) is an influential stream component. Once stable LWD obstructs streamflow and regulates key processes, causing increases in storage capacity, scouring, and variations to the bed, the extent contingent upon LWD’s average length of residence time within a system. Several North American studies have acknowledged the effects of interactions between wood, sediment, and flow regimes (Bilby, 1981; Keller, E.A., and Swanson, F.J., 1979; Montgomery et al., 1995; Wohl, E., 2008), linking the triad to geomorphic changes, the redistribution of bed materials, and ecological benefits. A consensual baseline reference for LWD’s function over time does not exist however, partly due to previous research being primarily conducted in the Northeast and Pacific Northwest regions where historic actions of humans, particularly riparian logging and stream clearing, have greatly impacted the condition of the watersheds. Researchers having long-overlooked the Great Plains and other regions not commonly associated with woody vegetation has increased the ambiguity regarding the transferability of LWD findings between regions. By shifting the focus to a non-forested region, the goal of this thesis is to measure the dynamics and influence of a prairie stream’s wood load on sediment storage and bed morphology. The Kings Creek network study area is located on the Konza Prairie Biological Station in northeastern Kansas, and drains one of few remaining unaltered North American watersheds. Results document the ongoing forest expansion into the surrounding pristine grassland, and provide a temporal context of the regions changing climate representative of atypical stream conditions caused by drought. In total, 406 individual pieces of wood were measured. The wood load was lower than most forest streams referenced (13.05 m[superscript]³/100 m), though higher than expected resulting from the absence of streamflow. LWD stored 108 m[superscript]³ of sediment within the channel, and the cumulative volume of LWD-formed pools was 169 m[superscript]³. Additionally, statistical analysis showed longitudinal bed variations to be strongly associated to LWD abundance, further indicating that LWD influences prairie stream processes similarly to those in a forest stream.

In boreal catchments, stream water chemistry is influenced and controlled by several landscape factors. The influence of spatially distributed variables is in turn dependent on the hydrological scale. Headwater streams have larger variability of water chemistry, and thus together represent a large biodiversity, and therefore need to be monitored in official environmental assessments. One objective of this study was, using Geographical Information Systems (GIS), to analyse co-variation between landscape variables and water chemistry and to determine which of the landscape variables have a major influence on the concentration of dissolved organic carbon (DOC) in headwater streams. Another objective was to find a simple method for predicting sources of DOC, using official map data and publically available GIS applications. Totally 85 headwater catchments (0.1-4 km2) in the county of Värmland, western south Sweden, were used in the study. Water chemistry was analysed for water sampled at low, medium and high flows, and landscape variables were extracted from official map data sources: topographic maps, a digital elevation model (DEM, 50 m grid), and vegetation data. Statistical analyses showed that topography (mean slope and mean topographic wetness index (TWI)) and wetland cover often correlated well with DOC in headwater catchments. Official map data could satisfactorily extract landscape variables (mean slope, mean TWI) that were useful in predicting stream water chemistry (DOC). A high-resolution elevation model, which was generated by interpolation of photogrammetric data, was used to calculate and evaluate two different wetness indices and their ability to predict the occurrence of wetlands in six catchments of different sizes and topography. The SAGA (System for Automated Geoscientific Analyses) wetness index (SWI) gave substantially better results than the TWI. The effects of resolution of DEMs on calculations of the SWI were investigated using 5, 10, 25 and 50 m grids. The results showed that SWI values increased with increasing cell size. The near linear increment of mean values for resolutions 10-50 m suggests a independence of terrain type and catchment size, which supported previous findings that indicated that mean slope and mean wetness index calculated from coarse elevation models may be used for prediction of DOC in headwater streams.

Made available in DSpace on 2014-06-11T19:35:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-02Bitstream added on 2014-06-13T20:46:50Z : No. of bitstreams: 1 souza_up_dr_rcla.pdf: 976950 bytes, checksum: b3702264109c8e9210e947b1e4df6b90 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo geral do presente estudo foi analisar a biologia e o ciclo de vida de Astyanax cf. scabripinnis paranae no ribeirão Grande, um riacho de cabeceira localizado no Parque Estadual da Serra do Mar (PESM), Núcleo Santa Virgínia, SP. Os exemplares foram coletados mensalmente de janeiro a dezembro em 2004 e 2006, utilizando-se redes de espera, redinha de mão e covo. Foram coletados dados de temperatura da água, oxigênio dissolvido e pH. Dados de pluviosidade, umidade relativa e temperatura do ar foram obtidos junto à Estação Meteorológica do PESM. Os peixes foram fixados em formalina a 10% e conservados em álcool 70%. No laboratório foram mensurados quanto ao comprimento total e padrão, peso e registrados os graus de repleção estomacal, de gordura acumulada, o sexo e os estádios de maturação gonadal. As correlações entre as variáveis ambientais e os meses de coletas foram verificadas por uma análise de componentes principais, que indicou uma separação entre períodos mais chuvosos (outubro a abril) e menos chuvosos (maio a setembro). A dieta foi analisada pelo grau de preferência alimentar. Diferenças no consumo de itens autóctones e alóctones e possíveis mudanças ontogenéticas ao longo do desenvolvimento da espécie foram verificadas por uma análise de correspondência. Para o estudo da reprodução foram analisadas as variações nos estádios de maturação, relação gonadossomática, grau de gordura acumulada, condição corporal, fecundidade e tipo de desova. O comprimento médio de primeira maturação gonadal foi estimado pelo ajuste não-linear e a condição corporal foi avaliada por modelos de análise de covariância. A fecundidade foi estimada pelo método volumétrico e relacionada ao comprimento padrão, ao peso total, ao peso das gônadas e à relação gonadossomática. No estudo do crescimento, a separação das coortes... / The present study aimed to analyze the biology and ecology of Astyanax cf. scabripinnis paranae in the Ribeirão Grande, a headwater stream located in the Parque Estadual da Serra do Mar (PESM), Núcleo Santa Virgínia, SP. The fishes were sampled monthly from January to December in 2004 and 2006, using gillnets, sieves and funnel traps. Temperature, dissolved oxygen and pH of the water were measured. Pluviometric data, relative humidity and temperature of the air were obtained in the Meteorological Station of PESM. The fishes were fixed in 10% formalin and conserved in 70% alcohol. In the laboratory they were measured as for the total and standard length, weighed and registered the degrees of stomach repletion, of accumulated fat, the sex and the stadiums of gonad maturation. The correlation structure among the environmental variables measured in the sampling collections was verified by a principal components analysis (PCA), which indicated a separation among rainier (October to April) and the less rainy periods (May to September). Differences in the consumption of autochthonous and allochthonous items and possible ontogenetic changes along the specimens development were verified by a correspondence analysis (CA). For the study of the reproduction the variations were analyzed at the maturation stadiums, gonadossomatic relationship, degrees of accumulated fat, corporal condition, fecundity and spawning type. The mean length of first gonad maturation was assessed by a no-linear adjustment. The corporal condition was evaluated by analysis of covariance. The fecundity was estimate by the volumetric method and related to the standard length, to the total weight, to the gonad weight and to the gonadossomatic relationship. In the study of the fish growth, the cohorts’ separation and their mean lengths were obtained using the package MIXDIST and the Bhattacharya method... (Complete abstract click electronic access below)

Orientador: Francisco Manoel de Souza Braga / Banca: Leandro Müller Gomiero / Banca: Lilian Casatti / Banca: Mauricio Cetra / Banca: Lucia Ap. de Fatima Mateus / Resumo: O objetivo geral do presente estudo foi analisar a biologia e o ciclo de vida de Astyanax cf. scabripinnis paranae no ribeirão Grande, um riacho de cabeceira localizado no Parque Estadual da Serra do Mar (PESM), Núcleo Santa Virgínia, SP. Os exemplares foram coletados mensalmente de janeiro a dezembro em 2004 e 2006, utilizando-se redes de espera, redinha de mão e covo. Foram coletados dados de temperatura da água, oxigênio dissolvido e pH. Dados de pluviosidade, umidade relativa e temperatura do ar foram obtidos junto à Estação Meteorológica do PESM. Os peixes foram fixados em formalina a 10% e conservados em álcool 70%. No laboratório foram mensurados quanto ao comprimento total e padrão, peso e registrados os graus de repleção estomacal, de gordura acumulada, o sexo e os estádios de maturação gonadal. As correlações entre as variáveis ambientais e os meses de coletas foram verificadas por uma análise de componentes principais, que indicou uma separação entre períodos mais chuvosos (outubro a abril) e menos chuvosos (maio a setembro). A dieta foi analisada pelo grau de preferência alimentar. Diferenças no consumo de itens autóctones e alóctones e possíveis mudanças ontogenéticas ao longo do desenvolvimento da espécie foram verificadas por uma análise de correspondência. Para o estudo da reprodução foram analisadas as variações nos estádios de maturação, relação gonadossomática, grau de gordura acumulada, condição corporal, fecundidade e tipo de desova. O comprimento médio de primeira maturação gonadal foi estimado pelo ajuste não-linear e a condição corporal foi avaliada por modelos de análise de covariância. A fecundidade foi estimada pelo método volumétrico e relacionada ao comprimento padrão, ao peso total, ao peso das gônadas e à relação gonadossomática. No estudo do crescimento, a separação das coortes... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The present study aimed to analyze the biology and ecology of Astyanax cf. scabripinnis paranae in the Ribeirão Grande, a headwater stream located in the Parque Estadual da Serra do Mar (PESM), Núcleo Santa Virgínia, SP. The fishes were sampled monthly from January to December in 2004 and 2006, using gillnets, sieves and funnel traps. Temperature, dissolved oxygen and pH of the water were measured. Pluviometric data, relative humidity and temperature of the air were obtained in the Meteorological Station of PESM. The fishes were fixed in 10% formalin and conserved in 70% alcohol. In the laboratory they were measured as for the total and standard length, weighed and registered the degrees of stomach repletion, of accumulated fat, the sex and the stadiums of gonad maturation. The correlation structure among the environmental variables measured in the sampling collections was verified by a principal components analysis (PCA), which indicated a separation among rainier (October to April) and the less rainy periods (May to September). Differences in the consumption of autochthonous and allochthonous items and possible ontogenetic changes along the specimens development were verified by a correspondence analysis (CA). For the study of the reproduction the variations were analyzed at the maturation stadiums, gonadossomatic relationship, degrees of accumulated fat, corporal condition, fecundity and spawning type. The mean length of first gonad maturation was assessed by a no-linear adjustment. The corporal condition was evaluated by analysis of covariance. The fecundity was estimate by the volumetric method and related to the standard length, to the total weight, to the gonad weight and to the gonadossomatic relationship. In the study of the fish growth, the cohorts' separation and their mean lengths were obtained using the package "MIXDIST" and the Bhattacharya method... (Complete abstract click electronic access below) / Doutor

Carbon Dioxide (CO2) emissions from streams are a significant component of the global carbon cycle.Terrestrial export of CO2 through runoff is increasingly recognized as a major source of CO2 in boreal headwater streams. However, the spatial and temporal distribution of soil water CO2 within theterrestrial landscape remains poorly quantified, contributing to large uncertainties about the origin of CO2 in headwater streams. The riparian zone (i.e. the area with fine sediments and organic rich soils closest to the stream) is accepted as a main contributor of organic carbon to streams, but its importanceas a source of CO2 is less evident. Here I evaluate the riparian zone as a main source by quantifying the contribution of lateral CO2 export from the riparian and hillslope zones to a headwater stream in a Swedish boreal catchment. Hourly measurements of CO2 concentration, conductivity, soil temperature and water table levels were taken in the riparian zone and the hillslope from June 2014 to October 2015. The riparian zone accounted for 58-89 % (August 2014 and March respectively) of the total terrestrial CO2 export from the slope to the stream. The hillslope, in turn, became a progressively larger source of CO2 to the stream during high flow events. To identify the drivers behind these zone-dependent and seasonal patterns in CO2 export, the CO2 production dissolved in the groundwater (groundwater- absorbed carbon) was estimated by taking the temporarily stored CO2  into account. The highest groundwater-absorbed carbon was observed during April and May (5.0 and 7.1 g C-CO2 m-2 month-1 respectively) which is the period with the highest discharge due to snow melt and the initiation of spring production. As such, conventional methods (gas chambers and the gradient method) may underestimate the soil respiration up to 50% during periods of high flow, as they exclude groundwater-absorbed carbon. CO2 consumption was observed in September 2014 and October 2015 (-0.2 and -0.7 g C-CO2 m-2 month-1 respectively) and may be explained by a major amount of the soil respiration being emitted instead of diluted in the groundwater during periods of low groundwater levels. It can be concludedthat, regardless of season, the riparian zone is a major source of CO2 to the headwater stream. / En signifikant mängd koldioxid (CO2) är lagrad i skog och marken. Marken i barrskogsregionernaförvarar en signifikant mängd CO2 där det partiella trycket av CO2 varierar mellan ~10 000 – 50 000 ppm i jämförelse med atmosfären (400 ppm). Mättnaden av CO2 gör att mycket avdunstar tillbaka till atmosfären. Dock absorberas en del CO2 av grundvattnet; vilket resulterar i en naturlig transport av CO2 vidare till ytvattnen där det kapillära nätverket av bäckar är största recipienten. Det är fortfarande oklart hur transporten av CO2 är distribuerad i ett vattenavrinningsområde vilket medför brister i förståelsen av en viktig processväg som kan komma att spela en större roll i framtidens kolkretslopp på grund av den globala uppvärmningen. Därför är en kvantifiering av olika områdens bidrag av CO2 till bäckarna nödvändig. Två betydande zoner i ett vattenavrinningsområde som troligen bidrar olika är: the riparian zone som är närmast bäcken och består av fina sediment med hög organisk halt och, the hillslope som är resterande område och består av grovkorniga jordar med låg organisk halt. Den förstnämnda misstänks transportera mer CO2 via grundvattnet på grund av dess närhet till bäcken, höga halter av CO2 och höga vattenmättnad men detta är ännu inte verifierat. Jag evaluerar the riparian zone som en viktig källa till CO2 i ett vattenavrinningsområde genom att kvantifiera transporten av CO2 från de två zonerna. För att förklara varför transporten varierar presenterar jag en ny modell (GVR) som beräknar den månatliga fluktuationen av den del av CO2-produktionen som absorberas i grundvattnet i the riparian zone. Mätningar av data utfördes i Västrabäcken, ett mindre vattenavrinningsområde i ett större vid namn Krycklan, i norra Sverige. En transekt av tre mätstationer (i bäcken, the riparian zone och the hillslope) installerades i den förmodade grundvattenströmningsriktningen. Resultaten visar på en hög produktion av CO2 under vårfloden (maj) då en hög grundvattenyta troligen absorberar en signifikant mängd CO2. Detta kan betyda att jordrespiration under våren underskattas då dagens mätmetoder är begränsade till mätningar i jorden av CO2 ovan grundvattenytan. Fortsatta studier rekommenderas där GVR-modellen och andra mätmetoder utförs samtidigt för att vidare utröna den kvantitativa underskattningen under perioder med hög grundvattenyta (speciellt under våren). Bidraget från the riparian zone till den totala laterala transporten av CO2 till bäcken under ett år varierar mellan 58-89 % och det månatliga transportmönstret kunde förklaras med resultaten från GVR-modellen. Resultaten verifierar att oberoende av säsong så är the riparian zone den huvudsakliga laterala koltransporten från landvegetationen; medan the hillslope procentuellt bidrar med mer CO2 under höga grundvattenflöden.