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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
51

Změny v preferencích heterozygotnosti MHC genů v průběhu menstruačního cykluu / Changes in preferences for heterozygosity in MHC genes across the menstrual cycle

Ptáčková, Kateřina January 2010 (has links)
5 Abstract Products of major histocompatibility complex (MHC) plays key role in immune system of vertebrates. Prior studies on different vertebrate species show, that heterozygosity in MHC genes is linked to more efficient immune system and preferred in mate choice. Results of human studies are ambivalent, which can be due to the effect of some modulating factors like reproductive status. Therefore, our aim was to test heterozygosity related preferences in faces, odor and voice across menstrual cycle. Our sample consisted of 51 men and 52 women, from which 23 used hormonal contraception and 29 had natural cycle. They were genotyped in -A, -B and -DR alleles. All odor stimuli, face photos and voice records were rated on seven-point scale in both follicular and luteal phase. Repeated measures ANOVA was used for the analysis. Changes in ratings across the menstrual cycle and heterozygosity were most discernible on voice ratings. Voices of homozygous males were rated more attractive than voices of heterozygous males especially in follicular phase. Similar shift to higher ratings in follicular phase was manifested in ratings of homozygous male faces, but the difference between homozygous males and heterozygous males was not significant. Women with natural cycle also rated voices higher in their follicular phase...
52

Population Genetics of Greater Sage-Grouse in Strawberry Valley, Utah

Dunken, Paula S. 01 July 2014 (has links) (PDF)
This study examined population genetics of greater sage-grouse (Centrocercus urophasianus) in Strawberry Valley, Utah located in the north-central part of the state. The Strawberry Valley population of sage-grouse experienced a severe population decline with estimates of abundance in 1998 less than 5% (~150 individuals) of similar estimates from the 1930s (>3,000 individuals). Given the population decline and reduced genetic diversity, recovery team partners translocated sage-grouse from four different populations into Strawberry Valley over 6 years (2003-2008). Translocations have been used as a strategy to increase both population size and genetic diversity in wildlife populations. We assessed whether genetic diversity increased following the translocation of sage-grouse into Strawberry Valley by looking at both nuclear and mitochondrial DNA indices. We observed an overall increase of 16 microsatellite alleles across the 15 loci studied (x̅ =1.04 alleles per locus increase, SE ± 0.25). Haplotype diversity increased from 4 to 5. Levels of genetic diversity increased for both nuclear and mitochondrial DNA (16% and 25% increases for allelic richness and haplotype diversity, respectively). These results show that translocations of greater sage grouse into a wild population can be an effective tool to increase not only population size but also genetic diversity.Second, we studied fitness-related traits and related them to genetic diversity indices in a population of greater sage-grouse in Strawberry Valley, Utah from 2005 to 2013. We captured 93 sage-grouse in Strawberry Valley and fitted them with a radio collar and drew and preserved blood. We monitored sage-grouse weekly, throughout each year. From blood, we extracted and amplified DNA with 15 microsatellite loci. We determined genetic diversity as multilocus heterozygosity and mean d2. To determine if there was a relationship between genetic diversity and survival, we used known-fate models in Program MARK. We also determined if there was a relationship between genetic diversity measures and nest initiation, nest success, clutch size, and number of eggs hatched using generalized linear models where reproductive measures were modeled as a function of genetic diversity. We found no significant relationship between mean d2 and microsatellite heterozygosity with measures of survival or reproductive fitness. Overall, these results suggest that the often-reported strong heterozygosity-fitness correlations detected in small, inbred populations do not reflect a general phenomenon of increasing individual survival and reproductive fitness with increasing heterozygosity.
53

Movements, relatedness and modeled genetic manipulation of white-tailed deer

Webb, Stephen Lance 11 December 2009 (has links)
White-tailed deer (Odocoileus virginianus) have been intensively studied across their range. However, many aspects of the white-tailed deer’s ecology have not been studied or are difficult to study. The advent of global positioning system (GPS) collar technology and molecular genetics techniques now allows researchers to collect fine-scale and cryptic phenomena. In addition, selective harvest of male white-tailed deer, based on antler size, has not been critically evaluated. Thus, development and use of quantitative genetics models will be useful for elucidating the effects of selective harvest on mean population antler size. I used GPS collar technology to further understand white-tailed deer movement ecology. First, I determined the efficacy and influence of a high-tensile electric fence (HTEF) on deer movements. The HTEF controlled deer movements when properly maintained and had little influence on deer spatial dynamics, making it a safe and cost-effective alternative to traditional fencing. Second, I studied fine-scale deer movements using GPS collars collecting locations every 15 minutes. Hourly deer movements were greatest in the morning and evening. Parturition and rut influenced movements of females and males, respectively whereas weather and moon phase had minimal influence on movements. Molecular genetics techniques are becoming more widespread and accessible, which may allow insight into the link between genetics and antler size. I found deer in 3 diverse populations from Mississippi, Oklahoma and Texas were relatively heterozygous and unrelated. Groups of deer with similar antler characteristics did not appear to be inbred or share common ancestors. In addition, there was not a strong link between individual multi-locus heterozygosity and antler points or score. Selective harvest has been implicated in causing negative evolutionary and biological responses in several ungulate species. To better determine how selective harvest (i.e., culling; the removal of deer with inferior antlers) affects white-tailed deer antler size, I used quantitative genetic models to simulate response of deer antlers to selection. In simulated controlled breeding situations response to selection was rapid, resulting in improvement in antler size. In simulated free-ranging populations response of antler size to selection was slow and only resulted in minimal increases in antler points after 20 years.
54

Analyse von Mikrosatelliteninstabilität und hMSH2-Expression bei Patienten mit akuter myeloischer Leukämie / Analysis of microsatellite instability and hMSH2 expression in patients with acute myeloid leukemia

Kohaus, Petra 20 June 2017 (has links)
No description available.
55

Avaliação genômica da infertilidade masculina idiopática por azoospermia não obstrutiva / Genomic assessment of idiopathic male infertility by nonobstructive azoospermia

Grangeiro, Carlos Henrique Paiva 10 April 2018 (has links)
Infertilidade conjugal é uma doença do sistema reprodutivo que acomete cerca de 20% dos casais e na qual o fator masculino responde por metade desses casos. A infertilidade masculina é um fenótipo complexo que abrange diferentes fatores. Os fatores genéticos envolvidos variam desde mutações pontuais, microdeleções no cromossomo Y, até alterações cromossômicas, como a Síndrome de Klinefelter. Mesmo após avaliação clínicolaboratorial detalhada, metade dos pacientes permanece sem a identificação de um fator causal, caracterizando a infertilidade idiopática. Nesse grupo, observamos com maior frequência os pacientes com falha espermatogênica primária, que clinicamente apresentam oligozoospermia grave ou azoospermia não obstrutiva (ANO) e, no qual, preponderam fatores genéticos ainda desconhecidos. Para auxiliar na compreensão de possíveis alterações genômicas, sejam as variantes de número de cópias (CNVs) ou as regiões de perda de heterozigosidade (LOHs), envolvidas com infertilidade masculina idiopática, 16 pacientes com ANO e 6 controles foram investigados pela técnica de hibridação genômica comparativa (aCGH) utilizando a plataforma 4x180 CGH+SNP Agilent® com análise dos dados pelo software Nexus 8.0. Não foram observadas diferenças significativas tanto no número, como no tamanho das alterações genômicas em ambos os grupos. Foram descritas 18 novas alterações genômicas com efeito sobre a produção espermática, distribuídas na forma de 12 ganhos, 3 perdas e 3 LOHs. Os ganhos mais significativos para o fenótipo azoospermia não obstrutiva foram descritos em 7q36.3, 17q21.33, Xq21.1 e Yp11.2. Nessas regiões, os genes com maior impacto sobre o fenótipo foram, respectivamente, SHH, COL1A1, COX7B e LINC00279. Ganhos envolvendo a sub-banda Yq11.223 e contendo cópias dos genes DAZ1 e DAZ4 foram considerados benignos. As três perdas detectadas em 2q31.1, 3p21.1-21.31 e 15q11.2, contendo, respectivamente, os genes DLX1, CACNA2D2 e representantes da família de receptores olfatórios foram consideradas relevantes. A análise das LOHs em fenótipos complexos é escassa e desafiadora. No presente trabalho, foram descritas 3 dessas alterações, localizadas em 1p31.1, 7q21.1 e 12q21.1-21.2 e compartilhadas por mais de um indivíduo infértil. A descrição dessas alterações genômicas contribui para a compreensão de mecanismos complexos e ainda pouco estudados, que resultam em azoospermia não obstrutiva decorrente da falha espermatogênica primária. / Infertility is a disease of the reproductive system that affects about 20% of all couples, with half of the cases being related to the male factor. Male infertility is a complex phenotype associated with an interaction of different factors. The genetic factors involved may range from point mutations, microdeletions on the Y chromosome to chromosomal changes such as Klinefelter syndrome. Even after detailed clinical-laboratory evaluation, the etiology may remain unknown in approximately half of the patients, and, in such cases, the infertility can be classified as idiopathic. This group of patients more frequently present with primary spermatogenic failure, with severe oligozoospermia or non-obstructive azoospermia (NOA). Nevertheless, the underlying genetic factors are still largely unknown. In order to better understand the potential genomic changes involved with idiopathic male infertility, sixteen patients with NOA and 6 controls were investigated in this study. Copy number variants (CNVs) and regions of loss of heterozygosity (LOHs) were assessed by array comparative genomic hybridization technique (aCGH), using the Agilent® 4x180 CGH + SNP platform. Data analyses was performed using Nexus 8.0 software. No significant differences between the groups were observed in relation to either the number or the size of the genomic changes. Eighteen new genomic alterations were described that were associated with sperm production (12 gains, 3 losses and 3 LOHs). The most important gains for the nonobstructive azoospermia phenotype were observed in 7q36.3, 17q21.33, Xq21.1 and Yp11.2. In these regions, the genes related to greatest impact on the phenotype were SHH, COL1A1, COX7B and LINC00279, respectively. Gains involving the Yq11.223 sub-band and containing copies of the DAZ1 and DAZ4 genes were considered benign. All 3 losses detected in 2q31.1, 3p21.1-21.31 and 15q11.2, containing, respectively, the DLX1, CACNA2D2 genes and representatives of the olfactory receptor family were considered relevant. Analysis of LOHs in complex phenotypes such as male infertility has been infrequently reported and is challenging. In the present study, three significants LOHs were found (1p31.1, 7q21.1 and 12q21.1-21.2) and were identified in more than one infertile individual. The description of these genomic alterations contributes to a better understanding of this complex and poorly explored mechanisms that results in non-obstructive azoospermia due to primary spermatogenic failure.
56

Perfil de variação no número de cópias do DNA e regiões de perda de heterozigose na susceptibilidade ao lúpus eritematoso sistêmico / DNA copy number variation and loss of heterozygosity profiles in susceptibility to systemic lupus erythematosus

Barbosa, Fernanda Bueno 20 July 2017 (has links)
O lúpus eritematoso sistêmico (LES) é uma doença autoimune com forte componente genético, caracterizada por inflamação crônica e produção de autoanticorpos. O objetivo deste trabalho foi determinar o perfil de variação no número de cópias (CNVs) e de regiões de perda de heterozigose (LOH) na patogênese do LES. A detecção de CNVs e LOH foi feita pela metodologia Cytoscan HD array em pacientes com LES (n = 23) e indivíduos saudáveis (n = 110). Devido à formação tri-híbrida da população brasileira, foi desenvolvido e validado um painel de 345 marcadores informativos de ancestralidade, a partir dados provenientes do próprio array, para estimar as proporções de ancestralidade individual e, em última instância, inseri-las nos modelos de regressão logística como variável de controle nas análises de distribuição de CNVs e LOH. O perfil de CNVs evidenciou que o número e o tamanho de duplicações são maiores nos indivíduos saudáveis do que nos pacientes com LES. Duplicações nos genes FCGR3B e ADAM3A foram descritas como fator de proteção ao LES, quando tais genes foram avaliados por PCR quantitativa em maior grupo amostral de pacientes (n = 135) e controles (n = 200). Além disso, mostrou-se o efeito sinérgico da presença da deleção em ambos os loci FCGR3B e ADAM3A no aumento do risco para desenvolver a doença. Deleções em pacientes com LES envolvendo os genes CFHR4, CFHR5 e HLA-DPB2, previamente descritos em associação com o LES na literatura, foram identificadas por array e confirmadas por PCR digital. O protocolo desenvolvido para identificação de variantes raras, resultou em um conjunto de 21 CNVs raras em pacientes com LES. Em relação às regiões de perda de heterozigose, não foram encontradas evidências de que o número médio e a extensão dos segmentos LOH seja diferente entre pacientes e indivíduos saudáveis. No entanto, os cromossomos 6 e 12 em pacientes exibem regiões de perda de heterozigose em maior quantidade e tamanho do que os de indivíduos saudáveis, além de apresentarem 17 segmentos LOH restritos ao grupo de pacientes com LES. Os resultados aqui descritos evidenciam que novos loci de susceptibilidade ao LES podem ser encontrados quando a distribuição de CNVs é analisada em todo o genoma, em que a investigação de sua relação com a patogênese pode contribuir para a compreensão da base genética da doença. / Systemic lupus erythematosus (SLE) is an autoimmune disease with a strong genetic background characterized by chronic inflammation and autoantibody production. The purpose of this study was to determine the copy number variation (CNV) and loss of heterozygosity (LOH) profiles in the susceptibility to SLE. The detection of CNVs and LOH was performed by the Cytoscan HD array methodology in SLE patients (n = 23) and healthy subjects (n = 110). Due to the tri-hybrid composition of the Brazilian population, a panel of 345 ancestral informative markers was developed and validated, based on data from the array itself, to estimate the proportions of individual ancestry and, ultimately, to insert them into the logistic regression models as a control variable in the analysis of CNV and LOH distribution. The CNVs profile showed that the burden and the size of duplications are higher in healthy individuals than in SLE patients. Duplications in FCGR3B and ADAM3A genes were described as a protective factor for SLE, when these genes were evaluated by quantitative PCR in a larger SLE (n = 135) and control (n = 200) groups. In addition, the synergistic effect of the presence of deletion in both FCGR3B and ADAM3A loci increase the risk of developing the disease. Deletions in SLE patients encompassing the CFHR4, CFHR5 and HLA-DPB2 genes, previously described in the literature in association to SLE, were identified by the array and confirmed by droplet digital PCR. The pipeline developed here for the identification of rare variants resulted in a set of 21 rare CNVs in SLE patients. Regarding the loss of heterozygosity regions, no evidence was found that the mean number and extent of LOH segments is different between patients and healthy individuals. However, the chromosomes 6 and 12 in SLE patients exhibit greater quantity and size of LOH than those of healthy individuals, besides showing 17 LOH segments restricted to the group of SLE. The results described here show that novel susceptibility loci to SLE can be found once the distribution of variants is analyzed throughout the genome, in which the investigation of its relation to the pathogenesis may contribute to the understanding of the genetic basis of the disease.
57

"Estudo das alterações dos microssatélites D6S251 e D6S252 no carcinoma basocelular esporádico" / Study of alterations in microsatellites D6S251 and D6S252 in sporadic basal cell carcinoma

Martinez, Marcos Antonio Rodrigues 29 March 2006 (has links)
Existe grande interesse na determinação das bases genéticas do carcinoma basocelular (CBC) que expliquem seu fenótipo pouco agressivo e comportamento metastático infreqüente. Investigamos a instabilidade de microssatélites (MSI) e perda de heterozigosidade (LOH) nos microssatélites D6S251 (6q14) e D6S252 (6q16) de CBCs esporádicos de alto e baixo risco histológico através da análise de bandas obtidas pelo gel de poliacrilamida após PCR em comparação com o tecido normal. Não houve alteração do microssatélite D6S252 nas 15 amostras estudadas. Para o microssatélite D6S251, houve alterações em 6 das 26 amostras estudadas (23,07%). MSI e LOH ocorreram em 46,15% das amostras de alto risco (respectivamente 15,38% e 30,76), o que sugere o provável envolvimento da região 6q14 na diferenciação histológica do CBC / A lot of interest lies in determining the genetic basis of basal cell carcinoma (BCC) to explain the lack of aggressive phenotype and infrequent metastatic behavior. We have analyzed the microsatellite instability (MSI) and loss of instability (LOH) in the D6S251 (6q14) and D6S252 (6q16) microsatellites patterns of histological low- high-risk sporadic BCC tumor samples using PCR-based assay in comparison with normal tissue. We have not found any alteration in D6S252 microsatellite 15 samples studied. We have encountered D6S251 alterations in 6 of 26 BCC samples (23.07%).MSI and LOH occurred in 46.15% of high-risk samples (15.38% and 30.76%), These results probably suggests participation of 6q14 region in histological differentiation of BCC
58

Análise do status somático dos genes MEN1, AIP e p27Kip1 em tumores de pacientes com neoplasia endócrina múltipla tipo 1 / Analysis of the status of somatic and p27Kip1 genes in tumors from patients with multiple endocrine neoplasia type 1

Moraes, Michelle Buscarilli de 06 June 2012 (has links)
Aproximadamente 80% dos casos com Neoplasia endócrina múltipla tipo 1 (NEM1) possuem mutações germinativas no gene supressor de tumor MEN1, que os predispõem a tumores nas glândulas paratireóides, pâncreas endócrino e hipófise, além de outros tumores não endócrinos. A tumorigênese dos mais de 20 diferentes tipos de neoplasias já descritas na NEM1 ocorre pela presença da mutação germinativa MEN1 associadas a um segundo evento mutacional nas células desses tecidos, levando à perda de heterozigose (LOH) do locus do gene MEN1 (11q13) e à inativação da proteína supressora de tumor codificada por esse gene, a proteína MENIN. Recentemente, mutações germinativas em outros genes foram descritas em casos com NEM1 sem mutações no gene MEN1. Esses novos genes (CDKN1A, CDKN1B, CDNK2B e CDKN2C) codificam proteínas envolvidas no controle do ciclo celular (p21, p27, p15 e p18), chamadas proteínas inibidoras de quinases dependentes de ciclinas. Outro gene, chamado AIP, que codifica uma proteína chaperona de mesmo nome, também foi recentemente descrito associado à NEM1. Esses trabalhos descreveram o papel desses novos genes na NEM1, em nível germinativo, entretanto não esclareceu se esses novos genes estão inativados nos tumores de pacientes com NEM1 com mutação MEN1. O presente estudo investigou, pela primeira vez, o status somático do gene p27Kip1 em pacientes com mutação MEN1 e identificou quatro possíveis perda de heterozigose (LOH) em tumores de paratireóides e pâncreas, sugerindo que além de 11q13LOH, os tumores NEM1 podem sofrer raras perdas adicionais do gene supressor tumoral p27Kip1. Essas são as primeiras evidências na literatura de um processo de tumorigênese multi-step na NEM1, envolvendo três eventos genéticos: 1- Mutação germinativa MEN1; 2- 11q13-LOH; 3- Perda somática do gene supressor de tumor p27Kip1/CDKN1B / Approximately 80% of cases with multiple endocrine neoplasia type 1 (MEN1) harbor a germline mutation in the tumor suppressor gene MEN1, which predisposes these patients to tumors comprehending the parathyroid and pituitary glands, endocrine pancreas and others non-endocrine tumors. The tumorigenesis of the more than 20 different types of tumors already described in the MEN1 syndrome occurs due to a MEN1 germline mutation associated with a second mutational event in the cells of these tissues, leading to loss of heterozygosity (LOH) of the MEN1 gene locus (11q13) and therefore inactivation of tumor suppressor protein encoded by this gene, MENIN protein. Recently, germline mutations in other genes have been described in cases with MEN1 without any detectable mutations in the MEN1 gene. These novel genes (CDKN1A, CDKN1B, CDNK2B and CDKN2C) encode proteins involved in the control of the cell cycle (p21, p27, p15 and p18), called cyclin dependent kinases inhibitors. Another gene, called AIP, which encodes a chaperon protein with the same name, was recently described associated with MEN1 phenotypes. These data described a role for these novel genes in the germline level, however whether they are inactivated in tumors of patients with MEN1 mutation is so far not clarified. The present study investigated for the first time, the somatic status of p27KIP1 gene mutation in patients with MEN1 and identified four possible loss of heterozygosity (LOH) in tumors of the parathyroid and pancreas, suggesting that in addition 11q13-LOH, MEN1 tumors may suffer rare loss additional tumor suppressor gene p27KIP1. These are the first evidence in the literature of a process of tumorigenesis in MEN1 multi-step, involving three genetic events: 1-MEN1germlinemutation; 2-11q13LOH; 3-Loss of somatic tumor suppressor gene p27Kip1/CDKN1B
59

Loss of heterozygosity on chromosome 1 in cervical cancer.

January 1998 (has links)
Poon Cho Sun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 83-91). / Abstract also in Chinese. / ACKNOWLEDGEMENT --- p.v / ABSTRACT --- p.vi / LIST OF ABBREVIATIONS --- p.x / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.5 / Chapter 2.1 --- Epidemiology and aetiology of cervical cancer --- p.5 / Chapter 2.1.1 --- Incidence and mortality --- p.5 / Chapter 2.1.2 --- Aetiology --- p.6 / Chapter 2.1.2.1 --- Oral contraceptive pills and cervical cancer --- p.7 / Chapter 2.1.2.2 --- Human papilloma virus (HPV) and cervical cancer --- p.7 / Chapter 2.1.2.3 --- Immunity and cervical cancer --- p.8 / Chapter 2.1.2.4 --- Socio-economic differences and cervical cancer --- p.9 / Chapter 2.1.2.5 --- Smoking and cervical cancer --- p.9 / Chapter 2.1.2.6 --- Male role and cervical cancer --- p.9 / Chapter 2.1.2.7 --- Nutrition and cervical cancer --- p.10 / Chapter 2.2 --- Oncogenes and tumour suppressor genes --- p.10 / Chapter 2.2.1 --- Oncogene --- p.10 / Chapter 2.2.2 --- Tumour suppressor gene --- p.13 / Chapter 2.2.3 --- Alterations of oncogene in cervical cancer --- p.16 / Chapter 2.2.4 --- Alterations of tumour suppressor genes in cervical cancer --- p.18 / Chapter 2.3 --- Alterations of chromosome 1 in cervical cancer --- p.19 / Chapter 2.3.1 --- Cytogenetic tudy --- p.19 / Chapter 2.3.2 --- Molecular genetic study --- p.21 / Chapter 2.4 --- Loss of heterozygosity (LOH) --- p.21 / Chapter Chapter 3 --- Materials and methods --- p.24 / Chapter 3.1 --- Materials --- p.24 / Chapter 3.1.1 --- Patients --- p.24 / Chapter 3.1.2 --- Specimens --- p.24 / Chapter 3.1.2.1 --- Blood samples --- p.24 / Chapter 3.1.2.2 --- Tumour tissue specimens --- p.24 / Chapter 3.1.3 --- Chemicals and reagents --- p.25 / Chapter 3.1.3.1 --- Chemicals --- p.25 / Chapter 3.1.3.2 --- Reagents --- p.27 / Chapter 3.1.3.3 --- Markers --- p.29 / Chapter 3.1.4 --- Major equipment --- p.33 / Chapter 3.2 --- Methodology --- p.33 / Chapter 3.2.1 --- DNA extraction --- p.33 / Chapter 3.2.2 --- DNA amplification --- p.35 / Chapter 3.2.2.1 --- Validation of PCR primers and optimisation of PCR condition --- p.35 / Chapter 3.2.2.2 --- End labelling of the primer by (γ-32p)ATP --- p.35 / Chapter 3.2.2.3 --- PCR for LOH detection --- p.36 / Chapter 3.2.2.4 --- Electrophoresis --- p.37 / Chapter 3.2.2.5 --- Gel dry and radioautography --- p.38 / Chapter 3.2.2.6 --- PCR analysis of the D1S80 and D1S76 loci --- p.39 / Chapter 3.3 --- Determination of Loss of heterozygosity (LOH) --- p.39 / Chapter 3.4 --- Statistical analysis --- p.40 / Chapter Chapter 4 --- Results --- p.41 / Chapter 4.1 --- LOH analysis in cervical cancer --- p.41 / Chapter 4.2 --- LOH and age in cervical cancer --- p.60 / Chapter 4.3 --- LOH and pathological grade in cervical cancer --- p.62 / Chapter 4.4 --- LOH and clinical stage in cervical cancer --- p.64 / Chapter 4.5 --- LOH and clinical status in cervical cancer --- p.66 / Chapter Chapter 5 --- Discussion --- p.68 / Chapter 5.1 --- Microsatellite markers --- p.69 / Chapter 5.2 --- PCR condition --- p.70 / Chapter 5.3 --- LOH in cervical cancer --- p.72 / Chapter 5.4 --- Correlation of LOH with clinico-pathologic characteristics of cervical cancer --- p.76 / Chapter 5.4.1 --- LOH and age --- p.78 / Chapter 5.4.2 --- LOH and clinical stage --- p.78 / Chapter 5.4.3 --- LOH and pathologic grade --- p.79 / Chapter 5.4.4 --- LOH and clinical status --- p.79 / Chapter Chapter 6 --- Conclusion --- p.80 / Chapter Chapter 7 --- References --- p.83
60

A instabilidade genômica como fator prognóstico e diagnóstico na progressão de queratose actínica para carcinoma espinocelular humano / Genomic instability as a prognostic and diagnostic factor on the progression of human actinic keratosis, to squamous cell carcinoma

Cabral, Luciana Sanches 19 June 2007 (has links)
A instabilidade genômica tem sido amplamente usada para caracterizar células cancerosas. Alterações genéticas em queratose actínica (QA) e carcinoma espinocelular (CEC) foram investigadas pelo método de random amplified polymorphic DNA (RAPD) e análise de microssatélites com o objetivo de encontrar marcadores moleculares para auxiliar o prognóstico e o diagnóstico médico. O DNA foi obtido de pacientes brasileiros cirurgiados e tratados no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, totalizando oito QAs, 24 CECs, e tecidos normais e/ou leucócitos correspondentes. Os microssatélites estudados foram D6S251, D6S252, D9S15, D9S50, D9S52, D9S180, D9S196, D9S280 e D9S287, tendo em vista a detecção de instabilidade genômica representada por perda de heterozigosidade (LOH) e instabilidade de microssatélites (MSI). Os \"primers\" usados para comparar os padrões de RAPD foram OPA-2, OPA-7, OPA-13, OPA-17, OPB-8, OPB-13, OPB-17 e OPB-19. Foi obtida correlação significativa na progressão de QA (1/8) para CEC (5/22) referente ao microssatélite D6S251. As diferenças nos padrões de DNA obtidos pelo método RAPD comparados aos controles foram maiores em lesões com maior grau de severidade segundo critério histológico. O mesmo padrão RAPD foi observado no controle e no tumor em 27% QA, 24% CEC I, 9% CEC II e 0% CEC III. Estes resultados mostram que o microssatélite D6S251 e o método de RAPD são informativos, podendo ser potenciais candidatos para auxílio no diagnóstico e prognóstico de QA e CEC. / Genomic instability has been widely used to characterize cancer cells. Genetic alterations in human actinic keratosis (AK) and squamous cell carcinomas (SCC) were investigated by the random amplified polymorphic DNA (RAPD) method, and microsatellite analysis. DNA was obtained from Brazilian patients diagnosed and treated in the School of Medicine of University of Sao Paulo out Clinics Hospital. Eight AKs, 24 SCCs, and 4 BCCs, matched to normal skin tissue and/or leukocytes were studied. Microsatellite patterns were obtained with primers specific to amplify D6S251, D6S252, D9S15, D9S50, D9S52, D9S180, D9S196, D9S280, and D9S287, in search of detection Loss of heterozygosity (LOH) and Microsatellite instability (MSI). The RAPD primers were: OPA-2, OPA-7, OPA-13, OPA-17, OPB-8, OPB-13, OPB-17, and OPB-19. A significant correlation was obtained regarding the progress of AK (1/8) to SCC (5/22) detected with the D6S251 microsatellite. DNA fingerprint obtained with RAPD primers were altered in increasing number of samples, according to their histological degree of differentiation. Similar RAPD patterns were observed in tumor and control in 27% AK, 24% SCC I, 9% SCC II, and zero SCC III. These results suggest microsatellite D6S251 and RAPD method to be potential tools in diagnosis and prognosis of AK and SCC.

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