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An investigation into the role of human mesoderm induction-early response 1 (hMI-ER1) in regulating a histone acetyltransferase, a chromatin remodeling enzyme /Blackmore, Tina, January 2004 (has links)
Thesis (M.Sc.)--Memorial University of Newfoundland, 2004. / Bibliography: leaves 82-89.
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Étude du rôle des méthyltransférases de la Lysine 20 de l’Histone H4 dans la dynamique de la chromatine au cours du cycle cellulaire / Study of Histone H4 Lysine 20 methyltransferases functions in chromatin dynamics during the cell cycleIzard, Fanny 21 November 2017 (has links)
Dans les cellules eucaryotes l’organisation de l’ADN en chromatine n’assure pas seulement la compaction de l’ADN dans le noyau mais sert aussi de structure dynamique qui permet la régulation des processus pour lesquels l’ADN est une matrice comme la transcription, la réplication et la réparation de l’ADN. L’unité de base de la chromatine est le nucléosome qui est constitué de 147pb d’ADN qui s’enroule autour d’un octamère d’histones. Le nucléosome possède une structure flexible régulée par les modifications post-traductionnelles d’histones. Les modifications d’histones contribuent à la régulation des fonctions du génome en modérant directement la structure de la chromatine ou bien via le recrutement de protéines spécifiques liant la chromatine. La lysine 20 de l’histone H4 (H4K20) peut être modifiée pour générer 3 niveaux de méthylation : mono- (me1), di- (me2), et tri-méthylation (me3), chaque niveau de méthylation est associé à des fonctions spécifiques. PR-Set7 (aussi appelée Set8, Setd8 ou KMT5A) est l’unique enzyme connue pour catalyser la mono-méthylation H4K20 alors que les niveaux de di- et tri-méthylation sont le résultat de l’activité des enzymes Suv4-20h qui requièrent la mono-méthylation H4K20 induite par PR-Set7 comme substrat. Ces enzymes sont essentielles puisque des études de Knock-Out ont montré que PR-Set7 et les Suv4-20h sont requises pour le développement de la souris et que leur perte dans des modèles cellulaires entraine des dommages ADN et des défauts de cycle cellulaire. Toutefois, les fonctions des différents niveaux de méthylation et des enzymes associées restent peu claires. Le travail réalisé pendant cette thèse a révélé que l’action concertée des enzymes PR-Set7 et Suv4-20h est requise pour le contrôle (i) de l’assemblage de l’hétérochromatine sur l’ADN naissant, (ii) de la liaison du pre-RC sur un groupe d’origines de réplication tardives nécessaires pour la réplication de régions d’hétérochromatine dans le cycle cellulaire suivant. Les deux fonctions dépendent de la conversion de la mono-méthylation H4K20 en tri-méthylation H4K20 et du recrutement spécifique de la protéine liant la méthylation H4K20 ORCA/LRWD1. Ainsi, la déplétion de PR-Set7 par siRNA entraine des défauts de compaction de la chromatine en interphase des cellules qui sortent de mitose, ce qui favorise la fixation non spécifique des sous-unités MCMs et ORCs des complexes pre-RC. Finalement et étant donné le rôle clé de la méthylation H4K20 dans la formation de l’hétérochromatine et la réplication, mon travail de thèse a contribué à révéler que la surexpression de PR-Set7 est un facteur de mauvais pronostic dans le myélome multiple et que l’inhibition de cette enzyme par des composés chimiques pourrait dans le futur avoir un grand intérêt pour le traitement du cancer. / In eukaryotic cells, the organization of DNA into chromatin not only ensures its compaction into nucleus, but also serves as a dynamic structure that offers a range of possibilities for regulating DNA transactions, such as transcription, DNA replication and repair. The basic unit of chromatin is the nucleosome, which is constituted of 147 bp of DNA wrapped with an octamer composed of histone proteins. This nucleosome structure is versatile showing distinct variations, including post-translational modifications of histone proteins. Histone modifications contribute to the regulation of genome functions by altering directly the nucleosome structure or through the recruitment of specific chromatin-binding proteins. In this regard, the lysine 20 of histone H4 (H4K20) can be modified to generate three different methylation states: mono- (me1), di- (me2), and trimethylation (me3), with a unique activity being coupled to the specific extent of methylation on this lysine residue. PR-Set7 (also known as SET8 or SETD8) is the sole enzyme that catalyzes H4K20me1, whereas H4K20me2 and H4K20me3 occur through the action of Suv4-20h, which requires PR-Set7-induced H4K20me1 as a substrate. These enzymes are essential since knockout studies have shown that both PR-Set7 and Suv4-20h are required for mouse development and their loss causes DNA damage and cell cycle defects. However, the functions of different H4K20 methylation states and the associated enzymes still remain poorly understood.The work carried out during this thesis reveals that the concerted activity of PR-Set7 and Suv4-20h is required for the timely control of (i) heterochromatin assembly on nascent DNA and (ii) the licensing of a critical subset of late-firing origins necessary for the replication of heterochromatin regions in the following cell cycle. Both functions depend on the conversion of H4K20me1 to H4K20me3 and the specific recruitment of the H4K20me-binding protein LRWD1/ORCA. Accordingly, siRNA-mediated PR-Set7 depletion triggers a defective interphase chromatin compaction in cells that exit of mitosis, which in turn favor a non-specific chromatin loading of ORC and MCMs subunits of pre-replication complexes. Finally and consistent with a key role of H4K20 methylation in heterochromatin formation and replication, my thesis work contributes to reveal that up-regulation of PR-Set7 is a poor prognosis factor in multiple myeloma and that its inhibition by specific chemical compounds might be a great interest for cancer treatment in near future.
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Mécanismes de régulation épigénétique chez l'insecte holocentrique ravageur de culture Spodoptera frugiperd, Lépidoptera, Noctuidae / Epigenetic regulation mecanisms in holocentric pest crop Spdoptera frugiperda, Lepidoptera, NoctuidaeNhim, Sandra 26 November 2018 (has links)
Chez les eucaryotes, l’ADN est empaqueté dans des complexes protéiques d’histones nommés nucléosomes qui assurent sa conformation. Cet arrangement est hétérogène à travers le génome et peut être dynamiquement modifié. La régulation de l’architecture chromatinienne joue un rôle essentiel dans la stabilité des génomes ainsi que la dynamique transcriptionnelle. Certaines régions qualifiées d’ ‘’heterochromatine constitutive’’ sont toutefois connues pour être maintenues à l’état condensé. Régionalisées aux extrémités et centres des chromosomes, l’hétérochromatine constitutive participe des fonctions télomériques et centromériques.Spodoptera frugiperda (S.fru, Lépidoptère, Noctuelle) est un ravageur de culture endémique du continent américain, récemment invasif dans le continent africain. Comme tous les Lépidoptères, S.fru est une espèce holocentrique dont le centromère est réparti le long des chromosomes et non restreint en un point unique. Cette disposition interroge sur l’établissement, la distribution ainsi que la fonction conservée de l’HC puisque cette dernière est principalement décrite pour être majoritairement localisée dans de larges régions péricentriques. Comprendre l’architecture chromatinienne chez S.fru peut avoir un intérêt en lutte biologique mais également permettre d’approfondir les connaissances en épigénétique chez un organisme non-modèle.Dans le cadre de la thèse, nous nous sommes demandés si la diméthylation de la lysine 9 de l’histone 3 (H3K9me2), marqueur de l’hétérochromatine constitutive, possédait un rôle conservé chez S.fru. Pour ce faire, nous avons comparé des données de ChIP-seq d’H3K9me2 sur cellules et larves entières après avoir annoté les gènes et l’ensemble des éléments répétés du génome, susceptibles d’être enrichis par cette marque. Parallèlement, des échantillons d’ARN-seq ont été étudiés afin de questionner le statut répressif de l’hétérochromatine constitutive. Nos résultats suggèrent un invariable maintien d’H3K9me2 dans les régions (sub)télomériques transcriptionnellement inactives ainsi qu’une forte association aux locus répétés d'ADN ribosomal (rDNA). Ces séquences ne constituent toutefois qu’une minorité des régions enrichies, le reste étant retrouvé dans des séquences répétées ainsi que dans le corps des gènes, indifféremment de leur état transcriptionnel. La persistante association d’H3K9me2 aux télomères et rDNA présagerait d’un maintien de la marque à proximité des centromères dont nous proposons un modèle d’établissement.La disposition de l’hétérochromatine constitutive questionne celle des régions euchromatiniennes, pauvres en nucléosomes, transcriptionnellement active et dynamiquement modifiées au cours du développement, du cycle cellulaire et des conditions environnementales. Afin de tester l’antagonisme de ces conformations, nous avons respectivement étudié la répartition des zones ouvertes et fermées du génome de la larve au stade L4 par approches de FAIRE-seq et de MAINE-seq. Ces structures ont été décrites dans la littérature pour être enrichies par de spécifiques modifications d’histones. Ainsi nous avons mis au point le protocole de native ChIP-seq d’H3K4me3 (marque active) et H3K9me2, H3K9me3, H3K27me3 (marques répressives). L’analyse en cours de l’ensemble de ces données de séquençages permettra d’avoir une vue intégrée de l’architecture chromatinienne au stade ravageur. / In eukaryotes, DNA is arranged in histones proteins complexes called nucleosomes that shape its conformation. This arrangement is heterogeneous across genomes and can be dynamically modified. Regulation of chromatin architecture plays an essential role in genome stability and transcription dynamics. Some regions named ‘’constitutive heterochromatin’’ are nonetheless known to remain highly condensed, regardless of conditions. Regionalized at extremities and chromosomes centers, constitutive heterochromatin contributes to telomeric and centromeric functions.Spodoptera frugiperda (S.fru, Lepidoptera, Noctuidae) is major crop pest in the Americas that recently invaded Africa. Like all Lepidopteran, S.fru is holocentric which means that its centromere is spread along chromosome and not restricted to a uniq point. This disposition question about establishment, distribution but also conserved function of constitutive heterochromatin since its usually and mainly localized in large pericentric regions.Deciphering chromatinian architecture in S.fru can be of interest in biological control but also allow to deepen epigenetic knowledge in a non-model organism.During my phD, we questionned the role of histone 3 lysine 9 demethylated (H3K9me2) in S.fru, a histone modification known in other yet described organisms to be a constitutive constitutive heterochromatinian hallmark.We compared H3K9me2 ChIP-seq data on cells and larvae after overall genomic functional annotation, potentially enriched for this mark. In parallel, RNA-seq samples were analyzed to question the putative repressive status of constitutive heterochromatin.Our results suggest an invariant retention of H3K9me2 in (sub)telomeric regions transcriptionally inactive but also a strong association of this mark in repeated ribosomal DNA locus (rDNA).These sequences constitutes nonetheless a minority of enriched regions since most of them regionalize in repeated sequences like transposons and tandem array but also gene bodies, independently of their transcriptional states.Persistent H3K9me2 association to telomeres and rDNA could predict of the conserved expression of this mark near centromeres. Based on literature and bioinformatics analysis, we proposed a model for S.fru holocentromeres.Constitutive heterochromatin questions euchromatin arrangement, described to be nucleosome poor, transcriptionally active and dynamically modified across development, cell cycle and environmental conditions. In order to test these structural antagonisms, we respectively studied open and closed genome conformations by FAIRE-seq and MAINE in larvae. These structures are reported to be associated to specific histones marks. We developed a native ChIP-seq protocol on H3K4me3 (active mark) and H3K9me2, H3K9me3, H3K27me3 (repressives marks). Overall analysis of these NGS data would help to picture an integrative view of chromatin architecture during larval pest stage.
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Characterizing the Molecular Changes of Austrofundulus limnaeus As It Develops Towards and Enters Diapause IIToni, Lee S. 12 1900 (has links)
Austrofundulus limnaeus is a species of annual killifish which inhabits ephemeral ponds in South America. The species is able to survive seasonally desiccating ponds due to their ability to produce robust embryos. The embryos of this species are capable of entering a developmental arrest, termed diapause II, which precedes the onset of drought. While in this arrested state embryos exhibit the greatest tolerance to anoxia of any characterized vertebrate at 25ºC. Furthermore, when raised at 30ºC, embryos escape the entrance to diapause II and go on to develop directly. Currently, little is known about the molecular mechanisms which induce and maintain this developmentally arrested state. In this study I have developed methods to analyze changes in histone modifications in the context of diapause II. Histone modifications were chosen due to their extreme conservation and well characterized role as modulators of gene expression in other systems. Results utilizing adapted immunobased assays show significant changes in the global amount of H3S10P, H3K27me and H3K4me, as the embryos progress from early embryogenesis through the exit of diapause. Additionally, it is revealed that there exists a degree of phenotypic plasticity with regards to the entrance into diapause II which is modulated by the environment (severe hypoxia 0.1% O2). This work builds a foundation for future histone modification studies and contributes the development of several tools to the field. This study contributes to a greater molecular understanding of the cue(s) which influence the remarkable phenomenon of obligate developmental arrest in a vertebrate embryo.
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Quantitative proteomics methods for the analysis of histone post-translational modificationsAbshiru, Nebiyu 09 1900 (has links)
Les histones sont des protéines nucléaires hautement conservées chez les cellules des eucaryotes. Elles permettent d’organiser et de compacter l’ADN sous la forme de nucléosomes, ceux-ci representant les sous unités de base de la chromatine. Les histones peuvent être modifiées par de nombreuses modifications post-traductionnelles (PTMs) telles que l’acétylation, la méthylation et la phosphorylation. Ces modifications jouent un rôle essentiel dans la réplication de l’ADN, la transcription et l’assemblage de la chromatine. L’abondance de ces modifications peut varier de facon significative lors du developpement des maladies incluant plusieurs types de cancer. Par exemple, la perte totale de la triméthylation sur H4K20 ainsi que l’acétylation sur H4K16 sont des marqueurs tumoraux spécifiques a certains types de cancer chez l’humain. Par conséquent, l’étude de ces modifications et des événements determinant la dynamique des leurs changements d’abondance sont des atouts importants pour mieux comprendre les fonctions cellulaires et moléculaires lors du développement de la maladie.
De manière générale, les modifications des histones sont étudiées par des approches biochimiques telles que les immuno-buvardage de type Western ou les méthodes d’immunoprécipitation de la chromatine (ChIP). Cependant, ces approches présentent plusieurs inconvénients telles que le manque de spécificité ou la disponibilité des anticorps, leur coût ou encore la difficulté de les produire et de les valider. Au cours des dernières décennies, la spectrométrie de masse (MS) s’est avérée être une méthode performante pour la caractérisation et la quantification des modifications d’histones. La MS offre de nombreux avantages par rapport aux techniques traditionnelles. Entre autre, elle permet d’effectuer des analyses reproductibles, spécifiques et facilite l’etude d’un large spectre de PTMs en une seule analyse. Dans cette thèse, nous présenterons le développement et l’application de nouveaux outils analytiques pour l’identification et à la quantification des PTMs modifiant les histones.
Dans un premier temps, une méthode a été développée pour mesurer les changements d’acétylation spécifiques à certains sites des histones. Cette méthode combine l’analyse des histones intactes et les méthodes de séquençage peptidique afin de déterminer les changements d’acétylation suite à la réaction in vitro par l’histone acétyltransférase (HAT) de levure Rtt109 en présence de ses chaperonnes (Asf1 ou Vps75).
Dans un second temps, nous avons développé une méthode d’analyse des peptides isomériques des histones. Cette méthode combine la LC-MS/MS à haute résolution et un nouvel outil informatique appelé Iso-PeptidAce qui permet de déconvoluer les spectres mixtes de peptides isomériques. Nous avons évalué Iso-PeptidAce avec un mélange de peptides synthétiques isomériques. Nous avons également validé les performances de cette approche avec des histones isolées de cellules humaines érythroleucémiques (K562) traitées avec des inhibiteurs d’histones désacétylases (HDACi) utilisés en clinique, et des histones de Saccharomyces cerevisiae liées au facteur d’assemblage de la chromatine (CAF-1) purifiées par chromatographie d’affinité. Enfin, en utilisant la méthode présentée précédemment, nous avons fait une analyse approfondie de la spécificité de plusieurs HATs et HDACs chez Schizosaccharomyces pombe. Nous avons donc déterminé les niveaux d’acétylation d’histones purifiées à partir de cellules contrôles ou de souches mutantes auxquelles il manque une HAT ou HDAC. Notre analyse nous a permis de valider plusieurs cibles connues des HATs et HDACs et d’en identifier de nouvelles. Nos données ont également permis de définir le rôle des différentes HATs et HDACs dans le maintien de l’équilibre d’acétylation des histones. Dans l’ensemble, nous anticipons que les méthodes décrites dans cette thèse permettront de résoudre certains défis rencontrés dans l’étude de la chromatine. De plus, ces données apportent de nouvelles connaissances pour l’élaboration d’études génétiques et biochimiques utilisant S. pombe. / Histones are highly conserved, basic proteins found in eukaryotic cell nuclei. They organize and package DNA strands into nucleosome core particles (NCPs), the fundamental repeating units of eukaryotic chromatin. The histones are subject to a wide variety of posttranslational modifications (PTMs) including acetylation, methylation and phosphorylation. These PTMs play an essential role in DNA-replication, transcription, and chromatin assembly. Alterations in histone PTM abundances have been implicated in several types of cancer. For example, the global loss of trimethylation at H4K20 and acetylation at H4K16 is a hallmark of human cancers. Thus, characterization of histone PTMs and their dynamics is extremely useful for elucidating normal cellular functions and molecular pathways that lead to diseases.
Traditionally, histone PTMs are analyzed using antibody-based approaches such as western blot and chromatin immunoprecipitation (ChIP) assays. These methods, however, suffer from several limitations including antibody cross-reactivity, epitope occlusion, and the cost and difficulty in producing and validating antibodies. Over the last decade, mass spectrometry (MS) has emerged as a powerful technique for the characterization and quantification of histone PTMs. MS offers several advantages over the traditional approaches including reproducibility, specificity, and ability to rapidly analyze numerous PTMs in a single experiment. In this thesis, the development and applications of novel analytical tools for the identification and quantification of histone PTMs are presented.
First, a method useful for measuring the global and site specific changes in histone acetylation is described. This method combines intact mass analysis and peptide sequencing approaches to study the global and site specific changes in histone acetylation during in vitro assays with yeast Rtt109 and its chaperone (Asf1 or Vps75). Second, a method for analysis of isomeric histone peptides is presented. This method combines a high resolution LC-MS/MS with a novel bioinformatics tool called Iso-PeptidAce to deconvolute mixed spectra of co-eluting isomeric peptides. We benchmarked Iso-PeptidAce using mixtures of synthetic isomeric peptides. We demonstrated its capability in histones isolated from human erythroleukemic (K562) cells treated with clinically relevant histone deacetylase inhibitors (HDACi) and in affinity-purified S. cerevisiae histones bound to chromatin assembly factor-1 (CAF-1). Third, by employing the above methods, an in-depth quantitative analysis of the substrate specificities of several fission yeast HATs and HDACs was assessed. We determined the acetylation site occupancy of multiple lysines in histones isolated from a control or mutant strains lacking specific HAT or HDAC activities. Our analysis identified several known and novel HAT and HDAC target sites. Our data also defined the division of labor between the different HATs and HDACs in maintaining the steady-state level of histone acetylation. Overall, we anticipate that the methods described in this thesis will address some of the existing challenges facing the chromatin field. Moreover, the data presented will provide valuable insights for future genetic and biochemical studies involving the fission yeast.
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Organic codes and their identification : is the histone code a true organic codeKühn, Stefan 03 1900 (has links)
Thesis (MSc)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Codes are ubiquitous in culture|and, by implication, in nature. Code
biology is the study of these codes. However, the term `code' has assumed
a variety of meanings, sowing confusion and cynicism. The rst aim of this
study is therefore to de ne what an organic code is. Following from this, I
establish a set of criteria that a putative code has to conform to in order
to be recognised as a true code. I then o er an information theoretical
perspective on how organic codes present a viable method of dealing with
biological information, as a logical extension thereof.
Once this framework has been established, I proceed to review several of
the current organic codes in an attempt to demonstrate how the de nition
of and criteria for identifying an organic code may be used to separate the
wheat from the cha . I then introduce the `regulatory code' in an e ort
to demonstrate how the code biological framework may be applied to novel
codes to test their suitability as organic codes and whether they warrant
further investigation.
Despite the prevalence of codes in the biological world, only a few have
been de nitely established as organic codes. I therefore turn to the main
aim of this study which is to cement the status of the histone code as a
true organic code in the sense of the genetic or signal transduction codes.
I provide a full review and analysis of the major histone post-translational modi cations, their biological e ects, and which protein domains are responsible
for the translation between these two phenomena. Subsequently
I show how these elements can be reliably mapped onto the theoretical
framework of code biology.
Lastly I discuss the validity of an algorithm-based approach to identifying
organic codes developed by G orlich and Dittrich. Unfortunately,
the current state of this algorithm and the operationalised de nition of
an organic code is such that the process of identifying codes, without the
neccessary investigation by a scientist with a biochemical background, is
currently not viable.
This study therefore demonstrates the utility of code biology as a theoretical
framework that provides a synthesis between molecular biology and
information theory. It cements the status of the histone code as a true
organic code, and criticises the G orlich and Dittrich's method for nding
codes by an algorithm based on reaction networks and contingency criteria. / AFRIKAANSE OPSOMMING: Kodes is alomteenwoordig in kultuur|en by implikasie ook in die natuur.
Kodebiologie is die studie van hierdie kodes. Tog het die term `kode' 'n
verskeidenheid van betekenisse en interpretasies wat heelwat verwarring
veroorsaak. Die eerste doel van hierdie studie is dus om te bepaal wat
'n organiese kode is en 'n stel kriteria te formuleer wat 'n vermeende kode
aan moet voldoen om as 'n ware kode erken te word. Ek ontwikkel dan 'n
inligtings-teoretiese perspektief op hoe organiese kodes `n manier bied om
biologiese inligting te hanteer as 'n logiese uitbreiding daarvan.
Met hierdie raamwerk as agtergrond gee ek `n oorsig van 'n aantal van
die huidige organiese kodes in 'n poging om aan te toon hoe die de nisie
van en kriteria vir 'n organiese kode gebruik kan word om die koring van
die kaf te skei. Ek stel die `regulering kode' voor in 'n poging om te wys
hoe die kode-biologiese raamwerk op nuwe kodes toegepas kan word om hul
geskiktheid as organiese kodes te toets en of dit die moeite werd is om hulle
verder te ondersoek.
Ten spyte daarvan dat kodes algemeen in die biologiese w^ereld voorkom,
is relatief min van hulle onomwonde bevestig as organiese kodes. Die hoofdoel
van hierdie studie is om vas te stel of die histoonkode 'n ware organiese
kode is in die sin van die genetiese of seintransduksie kodes. Ek verskaf 'n
volledige oorsig en ontleding van die belangrikste histoon post-translasionele modi kasies, hul biologiese e ekte, en watter prote endomeine verantwoordelik
vir die vertaling tussen hierdie twee verskynsels. Ek wys dan hoe
hierdie elemente perfek inpas in die teoretiese raamwerk van kodebiologie.
Laastens bespreek ek die geldigheid van 'n algoritme-gebaseerde benadering
tot die identi sering van organiese kodes wat deur G orlich en
Dittrich ontwikkel is. Dit blyk dat hierdie algoritme en die geoperasionaliseerde
de nisie van 'n organiese kode sodanig is dat die proses van die
identi sering van kodes sonder die nodige ondersoek deur 'n wetenskaplike
met 'n biochemiese agtergrond tans nie haalbaar is nie.
Hierdie studie bevestig dus die nut van kodebiologie as 'n teoretiese
raamwerk vir 'n sintese tussen molekul^ere biologie en inligtingsteorie, bevestig
die status van die histoonkode as 'n ware organiese kode, en kritiseer
G orlich en Dittrich se poging om organiese kodes te identi seer met 'n algoritme
wat gebaseer is op reaksienetwerke en `n kontingensie kriterium.
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Régulation du gène "steroidogenic acute regulatory protein" par le cholestérol dans l'ovaire porcinDeneault, Eric January 2003 (has links)
Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.
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Caracterização e funcionalidade das enzimas modificadoras de histona desacetilases (HDAC) e arginina peptidil deiminase 4 (PADI4) no desenvolvimento embrionário /Oliveira, Clara Slade. January 2012 (has links)
Orientador: Joaquim Mansano Garcia / Coorientador: Flavia Lombardi Lopes / Coorientador: Daniel Robert Arnold / Banca: Flávio Vieira Meirelles / Banca: Luiz Sérgio de Almeida Camargo / Banca: Gisele Zoccal Mingoti / Banca: Naiara Zoccal Saraiva / Resumo: Modificações pós-translacionais de histonas são importantes componentes do código epigenético, e contribuem para o controle da transcrição gênica de cada célula. O presente trabalho descreve a participação de duas modificações de histonas no desenvolvimento embrionário pré-implantacional: a acetilação de lisinas e a citrulinização de argininas. No capítulo 1, avaliamos a presença de duas modificações de histona H3, K9ac (permissiva) e K27me3 (repressiva) em embriões bovinos no ciclo de ativação do genoma embrionário (AGE), por imunofluorescência. Ambas as marcas estão presentes e apresentam alto coeficiente de correlação, e dois perfis de embriões (com alta e baixa variação dos níveis das modificações entre blastômeros) foram descritos. A acetilação de histonas está relacionada à ativação da expressão gênica, portanto no capítulo 2 hipotetizamos que a manipulação de seus níveis em embriões bovinos poderia influenciar a ativação do genoma embrionário, o desenvolvimento de blastocistos e a inativação do cromossomo X em fêmeas. Foram testadas concentrações do inibidor das histona desacetilases tricostatina A variando de 5 a 50nM, aplicadas por 12 a 144h, iniciando 70h após a FIV. Três protocolos foram selecionados: 5nM 48h, 5nM 144h e 15nM 48h. Após, foi utilizado sêmen sexado para estudar os efeitos da TSA sobre embriões fêmeas e machos separadamente. Por imunofluorescência para H3K9ac, foi observado aumento na acetilação de histonas em ambas as concentrações (5 e 15nM), sendo 5nM mais eficaz em fêmeas do que machos. O tratamento com 15nM 48h reduziu a produção de blastocistos em machos e fêmeas, e 5nM 144h em machos. A taxa de apoptose, avaliada pelo ensaio TUNEL, foi elevada em embriões fêmeas (grupos 5nM144h e 15nM48h), e em machos (grupo 15nM48h), mas tal aumento... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Histone post translational modifications are important components of the epigenetic code, and contribute to the control of gene transcription in each cell. This work describes the participation of two histone modifications in embryonic preimplantation development: acetylation of lysines and citrullination of arginines. In chapter 1, we evaluated the presence of two modifications of histone H3, K9ac (permissive) and K27me3 (repressive) in bovine embryos in the cycle of embryonic genome activation (EGA), by immunofluorescence. Both marks are present and show a high correlation coefficient, and two profiles of embryos were described, displaying high and low variation of modifications level between blastomeres. The acetylation of histones is related to gene expression activation, so in Chapter 2 we hypothesized that the manipulation of acetylation levels in bovine embryos could influence embryonic genome activation, blastocyst development and X chromosome inactivation in females. Five concentrations of the histone deacetylase inhibitor trichostatin A (TSA), ranging from 5 to 50nM beginning 70 hours after FIV were applied per 12 to 144h. Three protocols were selected: 5nM 48h, 5nM 144h and 15nm 48h. After, sexed semen was used to study the effects of TSA on male and female embryos separately. Immunofluorescence of H3K9ac showed increased histone acetylation at both concentrations (5 and 15nM). 5nM TSA was more effective in females than in males. Treatment with 15nM 48h reduced male and female blastocyst yield, and 5nM 144h reduced male blastocyst yield. Apoptosis rate was measured by the TUNEL assay. Female 5nM144h and 15nM48h groups, and male 15nM48h group displayed higher apoptosis levels, but this increase was not observed in low quality embryos. In female embryos, TSA did not affect... (Complete abstract click electronic access below) / Doutor
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Histonas de glândulas salivares de Rhynchosciara americana: caracterização e síntese / Histone salivary glands Rhynchosciara americana: characterization and synthesisPueyo, Manuel Troyano 26 May 1976 (has links)
Histonas de glândulas salivares de larvas de Rhynchosciara americana foram extraídas de preparações de núcleos e de cromatina purificada e analisadas por eletroforese em géis de acrilamida. As mobilidades eletroforéticas das histonas ARE e GRR são idênticas e as das histonas KAP, LAR e KAS são levemente diferentes das histonas correspondentes encontradas em vertebrados. É particularmente interessante o comportamento da histona KAP de R. americana, que possui em géis de poliacrilamida mobilidade menor que a histona correspondente do timo de bezerro. Verificou-se, por análise em géis de poliacrilamida-SDS que o peso molecular desta histona é igual a 33.000 daltons, valor maior que o da histona correspondente de timo de bezerro e de outros vertebrados (21.000 a 25.000 daltons). Este resultado coincide com dados obtidos por outros investigadores em Drosophila e Acheta. Isto sugere que o maior peso molecular da histona KAP possa ser uma característica geral dos insetos. Verificou-se, por experimentos da dupla marcação com lísina- 14 C e triptofano-3 H, que a síntese de histonas em glândulas salivares de larvas de R. americana, ocorre no citoplasma em pequenos polirríbossomos contendo 3-4 ribossomos. Estes polirribissomos são particularmente ativos na síntese de histonas por ocasião da abertura máxima do puff B-2 na extremidade proximal da glândula,quando é máxima a síntese de DNA. O estudo da síntese das várias frações de histonas durante os 6-7 dias finais do período larval mostra um estreito acoplamento entre a síntese dessas frações e a do DNA. Não foram detectadas variações relativas na síntese de nenhuma das frações de histonas durante este período. Os resultados deste estudo sugerem também que algumas das frações de histonas são modificadas durante o desenvolvimento. Estas modificações podem ser correlacionadas com o aparecimento dos \"puffs de DNA\" nos cromossomos das células glandulares. Foi também estudada a síntese de histonas em larvas jovens (fim do segundo período do 4o estádio) injetadas com ecdisterona. Observou-se, conforme esperado de resultados anteriores, que a injeção do hormônio causou grande estímulo na síntese de DNA. Contudo, não houve estímulo apreciável na síntese de histonas. Estes resultados sugerem que a cromatina formada nestas condições é deficiente em histonas. Nestas experiências notou-se também, que após tratamentos com hormônio durante 44 horas ocorre uma profunda modificação nos perfis de radioatividade de histonas recém sintetizadas analisadas em géis de poliacrilamida. Sugere-se que a alteração desses perfis se deva à modificação química de alguma histona induzida pelo hormônio. / The histones of the salivary gland of Rhynchosciara americana larvae were extracted from both nuclearand purified chromatin preparations and then analyzed by polyacrylamide gel electrophoresis.The electrophoretic mobilities of the ARE and GRK histones are identical and those from the KAP, LAK and KAS histones are slightly different from the corresponding calf thrymus histones The behavior of the Rhynchosciara KAP histone is particularly interesting. In normal polyacrylamide gels it has a smaller mobility than that observed for the same protein from calf thymus.It was found by analysis in SDS-polyacrylamide gels that the molecular weight for this histone is approximately 33000 dalton, a value which is higher than that found for the same histone obtained from calf thymus and other vertebrates (21-250 00 dalton). This result is in agreement with those observed in Drosophila and Acheta, and suggests that the molecular weight of the KAP histone could be a characteristic feature for insects. Double label experiments with 14C-lysine and 3H-tryptophan show that histone synthesis in Rhynchoschiara salivary glands occurs in the cytoplasm in small polyribosomes containing 3-4 ribosomes. These polysomes are particularly active in the synthesis of these proteins at the time when the degree of opening of the 2B puff is maximal in the proximal cells of the gland, i.e when the DNA synthesis in the gland reach a maximum. The study of the synthesis of the different histone fractions during the last 6-7 days of the larval period shows a tight coupling between the synthesis of these fractions and that of DNA. However, variations in the relative proportions of those proteins during this period of larval development was not detected. The results of this study also suggest that a histone or histones are chemically modified during the development. These modifications can be correlated with the appearance of DNA puffs in the salivary gland. The study of histone synthesis was also carried out with younger larvae (end of the 2nd period of the 4th instar) treated with ecdysterone. It was found, as expected from previous results, that the hormone caused a great increase in the rate of DNA synthesis in the gland. Despite this, there was not an appreciable increase in the amount of newly synthesized histones which can be extracted from gland chromatin. These results indicate that the chromatin formed in the gland under these conditions might be deficient in histones In these experiments it was observed a great modification in the radioactivity profile of newly synthesided histones as analysed by polyacrylamide gel electrophoresis. This change occurs 44 hours after ecdysterone treatment. Tentatively it is suggested that this alteration in the radioactivity profiles would be a result of a chemical modification of some histone fraction which was induced by the hormone
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Understanding the soybean response to salinity stress: from the viewpoint of proteomics and histone modifications. / CUHK electronic theses & dissertations collectionJanuary 2010 (has links)
Histone modifications and histone variants are of importance in many biological processes. Whether they play some roles in regulating soybean salinity stress response is unknown. Previously, no study of histone modifications and histone variants in soybean were reported. In this study, I elucidated that in soybean leaves, mono-, di- and tri-methylation at Lysine (K) 4, 27 and 36, and acetylation at Lysine 14, 18 and 23 were present in histone H3. Moreover, H3K27 methylation and H3K36 methylation usually excluded each other. Although H3K79 methylation was not reported in Arabidopsis, they were detected in soybean. In soybean histone H4, Lysine 8 and 12 were acetylated. In addition, the variants of histone H3 and H4 and their modifications were also determined. The variants of histone H3 were different at positions of A31F41S87S90 (histone variant H3.1) and T31Y41H87 L90 (histone variant H3.2), respectively. Lysine 4 and 36 methylation were only detected in histone H3.2, suggesting that histone variant H3.2 might associate with actively transcribing genes. The two variants of histone H4 (H4.1 and H4.2) were different at amino acid 60. Moreover, I also found that the abundance of most of the histone modifications and histone variants did not change under the salinity stress except that H3K79 methylation would be up-regulated by the salinity stress. / In a parallel study, a PHD (plant homeodomain) finger domain containing protein, GmPHD1, was able to decipher the 'code' underlying H3K4 methylation. GmPHD1 was ubiquitously expressed in soybean and its expression increased upon salinity stress. GmPHD1 could bind to histone H3K4 methylation, with the preference to H3K4 dimethylation. It could then recruit several proteins, which were GmGNAT1, GmElongin A, and GmISWI. The interaction between GmPHD1 and GmGNAT1 was regulated by the self-acetylation of GmGNAT1. GmGNAT1 could also acetylate histone H3; GmElongin A was a transcription elongation factor; and GmISWI was a chromatin remodeling protein. Our data also indicated that the GmPHD1 located at the promoter of several soybean salt stress inducible genes. Therefore, the GmPHD1 recruited proteins to remodel the chromatin structure and facilitate the transcription of those salt stress inducible genes. Moreover, GmGNAT1 exhibited the preference to acetylate histone H3K14, therefore representing a kind of histone crosstalk between H3K4 methylation and H3K14 acetylation. / Proteomics studies with 2-DE revealed that salt treatment may affect soybean photosynthesis and chloroplast formation. Comparison between the proteomic profiles of salt tolerant soybean variety (wild type) and salt sensitive soybean variety (cultivated, Union) indicated that protein levels in the detoxification and defense pathway as well as energy metabolism were higher in the wild type soybean, while the process of protein metabolism was less active. In addition, proteomic profiles of the cultivated soybean roots at different developmental stages were also compared to identify proteins related to soybean development. The expression of proteins which play critical roles in detoxification and defense pathways were higher at the seedling stage, especially the proteins which regulated the formation of ROS. / Soybean is an important economic crop and its production can be severely affected by salinity stress. At present, the soybean response to salinity stress is not clear. In my studies, I tried to understand this process from the perspective of proteomics and epigenetics, especially histone modifications. / Wu, Tao. / Advisers: Njai Sai Ming; Lam Hon Ming. / Source: Dissertation Abstracts International, Volume: 73-02, Section: B, page: . / Thesis (Ph.D.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 126-151). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.
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