Come and meet Bee Bot—a friendly, easy-to-operate robot that engages students in learning math. We will present ideas for using Bee Bots to teach standards in the K-2 curriculum, with videos of them in action in the classroom. Free resources will be shared to bring this learning back to your classroom to excite your students

Come to this session to find out how you can meet demands to introduce coding in the K-2 classroom while still teaching your math standards!

Kontrollbalansräkningen är ett borgenärsskydd vars funktion är att särskilja livsdugliga företag från de som inte är det. Frågan om livsduglighet avgörs av företagets nettotillgångar. Vilka tillgångar som tas upp och hur tillgångarna värderas får därmed en avgörande betydelse för ett aktiebolags fortsätta existensen. Lagen ger ingen närmare beskrivning för hur redovisning av tillgångar ska ske, utan hänvisar till god redovisningssed. Vad som utgör god redovisningssed blir således viktigt för att avgöra nettotillgångarnas storlek och i förlängingen företags livsduglighet. Särskilda problem inom redovisningen återfinns vid värdering av immateriella tillgångar. Studiens syfte är därför att undersöka hur immateriella tillgångar kan klassificeras och värderas enligt god redovisningssed i kontrollbalansräkningar.För att besvara studiens syfte gjordes en kvalitativ innehållsanalys. Dokument som ansågs svara för god redovisningssed i form av RedR 2, K2, K3 samt rättsfall analyserades för att undersöka vad som torde vara de lege lata vid redovisning av immateriella tillgångar i kontrollbalansräkningar.Vår slutsats är att företag som i den ordinarie redovisningen tillämpar K2-regelverket i kontrollbalansräkningar har rätt att välja att redovisa immateriella tillgångar enligt K3-regelverket. Immateriella tillgångar med ett påvisbart försäljningsvärde som i den ordinarie redovisningen inte får tas upp kan redovisas i en kontrollbalansräkning. Utöver ovan slutsatser har även framförts vad som borde gälla de lege ferenda, då immateriella anläggningstillgångar i vissa fall torde kunna värderas till nyttjandevärde. / Program: Civilekonomprogrammet

Made available in DSpace on 2014-12-17T14:56:21Z (GMT). No. of bitstreams: 1 AdersonCP.pdf: 441948 bytes, checksum: 3ac355b4df6f67d2c5c0a9bb8f35c95a (MD5) Previous issue date: 2006-08-30 / Bayesian networks are powerful tools as they represent probability distributions as graphs. They work with uncertainties of real systems. Since last decade there is a special interest in learning network structures from data. However learning the best network structure is a NP-Hard problem, so many heuristics algorithms to generate network structures from data were created. Many of these algorithms use score metrics to generate the network model. This thesis compare three of most used score metrics. The K-2 algorithm and two pattern benchmarks, ASIA and ALARM, were used to carry out the comparison. Results show that score metrics with hyperparameters that strength the tendency to select simpler network structures are better than score metrics with weaker tendency to select simpler network structures for both metrics (Heckerman-Geiger and modified MDL). Heckerman-Geiger Bayesian score metric works better than MDL with large datasets and MDL works better than Heckerman-Geiger with small datasets. The modified MDL gives similar results to Heckerman-Geiger for large datasets and close results to MDL for small datasets with stronger tendency to select simpler network structures / Redes Bayesianas s?o poderosas ferramentas de representa??o gr?fica de distribui??es de probabilidade. Tais redes manipulam incertezas existentes em sistemas do mundo real. A partir da ?ltima d?cada, especial interesse no aprendizado de sua estrutura a partir de um conjunto de dados. Entretanto, o aprendizado da estrutura ? um problema NP-Dif?cil, o que gerou a cria??o de Algoritmos heur?sticos de busca. Muitos desses Algoritmos s?o baseados em m?tricas de pontua??o para estimar o modelo. Este trabalho procura comparar tr?s das m?tricas mais utilizadas. Para gerar os resul tados foram utilizadas as redes ASIA e ALARM, que s?o dois dos benchmarks padr?es e o Algoritmo de busca K-2. A m?trica Bayesiana Heckerman-Geiger com hiperpar?metros que dificultam a gera??o de arestas apresentam melhores resultados que ?quelas que flexibilizam a gera??o de arestas, acontecendo o mesmo com a m?trica MDL modificada. A compara??o das duas m?tricas mostrou que a m?trica Bayesiana ? superior ? m?trica MDL com grandes conjuntos de dados e inferior, caso contr?rio. A modifica??o na m?trica MDL resultou em estruturas mais pr?ximas ?s apresentadas pela MDL para um conjunto reduzido de dados e mais pr?ximas ? Heckerman-Geiger para um grande conjunto de dados, quando seus par?metros restrigem a cria??o de arestas

No description available.

<p>Silicon-based electric chip arrays were developed for characterization of Bacillus</p><p>cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. The purpose of this work was to develop a fast method for determination of the presence of these genes in colonies from primary enrichment cultures. The specific DNA detection was based on immobilization of DNA capture probes, which hybridize to specific sites on the target genes. Biotin-labeled detection probes were designed to hybridize with the target DNA adjacent to the capture probes. An extravidin - alkaline phosphatase complex was subsequently bound to the hybridized detection probes. Finally, p-aminophenyl phosphate was added as substrate for the enzyme, and the product p-aminophenol was brought in contact with the interdigitated gold electrode on the silicon chips surface. The p-aminophenol was oxidized at the anode to quinoneimine, which was then reduced back to paminophenol at the cathode. This redox recycling generates a current that was used as the DNA-chip response to the target DNA. Two versions of the assay were used. In the first version the capture probes were immobilized on magnetic beads and all</p><p>chemical reactions until and including the enzymatic reaction took place in an</p><p>eppendorf tube while the redox recycling was used to measure the amount of paminophenol produced after transfer from the tube to the silicon chip surface. In the second version a silicon chip array was used with 16 parallel electrode positions, each activated by immobilization of one type of capture probes on the gold electrodes. With this system all chemical reactions took place at the chip surface. The kinetics of cell disruption and DNA fragmentation from B. cereus by ultrasonication was determined. Maximum cell disruption was achieved within 5 min and the chip response increased in proportion to the ultrasonic time. Further ultrasonication up to 10 min resulted in further increasing current although no further cell disruption was observed. If the sonication time was extended above 10 min the signal declined. Based on analysis of the DNA size distribution by early end-point PCR and gel electrophoresis, it is suggested that the first 5 min ultrasonication increased the signal by increasing the release of target DNA molecules. Thereafter the signal was increased by fragmentation of target DNA which increases the diffusion rate and also the accessibility of the hybridization site. Finally, the DNA fragment sizes approached that of the hybridization site (51-bp) which may reduce the signal because of cleavage of the target DNA in the hybridization region. These studies were performed with the bead-based hybridization assay. The assay was highly specific to the target gene (hblC) of both B. cereus and B. thuringiensis with no response from negative control</p><p>cells of B. subtilis. The 16 positions of the silicon chip array were activated by</p><p>immobilization of all known toxin-coding genes of B. cereus and also included both a positive control and a negative control electrode positions. When these chips were exposed to ultrasonicated B. cereus, the gold electrodes were fouled by some component in DNA cell lysates. To circumvent this, the released large DNA was first extracted and then ultrasonicated again, since the extract mainly contains large molecular weight DNA. This DNA extract was applied to characterize one “diarrheal” and one “emetic” strain of B. cereus with the DNA chip arrays. The results agreed with PCR control analysis which means that these electric DNA chip arrays can be used to characterize bacterial colonies with respect to the genes coding of all known toxins of B. cereus: haemolysin (hblA, hblC, hblD), non-haemolytic enterotoxin (nheA, nheB, nheC), cytotoxin K-2 (cytK-2), and cereulide (ces). The chip assay required about 30 min after application of DNA samples. Due to the generic properties of the chips, this technique should also be applicable for characterization of the pathogenicity potential of many other organisms. Keywords: Bacillus cereus, haemolysin, non-haemolytic enterotoxin, cytotoxin K-2, cereulide, toxin-coding genes, bacterial colony, electric DNA chip, ultrasonication, DNA fragmentation.</p>

Silicon-based electric chip arrays were developed for characterization of Bacillus cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. The purpose of this work was to develop a fast method for determination of the presence of these genes in colonies from primary enrichment cultures. The specific DNA detection was based on immobilization of DNA capture probes, which hybridize to specific sites on the target genes. Biotin-labeled detection probes were designed to hybridize with the target DNA adjacent to the capture probes. An extravidin - alkaline phosphatase complex was subsequently bound to the hybridized detection probes. Finally, p-aminophenyl phosphate was added as substrate for the enzyme, and the product p-aminophenol was brought in contact with the interdigitated gold electrode on the silicon chips surface. The p-aminophenol was oxidized at the anode to quinoneimine, which was then reduced back to paminophenol at the cathode. This redox recycling generates a current that was used as the DNA-chip response to the target DNA. Two versions of the assay were used. In the first version the capture probes were immobilized on magnetic beads and all chemical reactions until and including the enzymatic reaction took place in an eppendorf tube while the redox recycling was used to measure the amount of paminophenol produced after transfer from the tube to the silicon chip surface. In the second version a silicon chip array was used with 16 parallel electrode positions, each activated by immobilization of one type of capture probes on the gold electrodes. With this system all chemical reactions took place at the chip surface. The kinetics of cell disruption and DNA fragmentation from B. cereus by ultrasonication was determined. Maximum cell disruption was achieved within 5 min and the chip response increased in proportion to the ultrasonic time. Further ultrasonication up to 10 min resulted in further increasing current although no further cell disruption was observed. If the sonication time was extended above 10 min the signal declined. Based on analysis of the DNA size distribution by early end-point PCR and gel electrophoresis, it is suggested that the first 5 min ultrasonication increased the signal by increasing the release of target DNA molecules. Thereafter the signal was increased by fragmentation of target DNA which increases the diffusion rate and also the accessibility of the hybridization site. Finally, the DNA fragment sizes approached that of the hybridization site (51-bp) which may reduce the signal because of cleavage of the target DNA in the hybridization region. These studies were performed with the bead-based hybridization assay. The assay was highly specific to the target gene (hblC) of both B. cereus and B. thuringiensis with no response from negative control cells of B. subtilis. The 16 positions of the silicon chip array were activated by immobilization of all known toxin-coding genes of B. cereus and also included both a positive control and a negative control electrode positions. When these chips were exposed to ultrasonicated B. cereus, the gold electrodes were fouled by some component in DNA cell lysates. To circumvent this, the released large DNA was first extracted and then ultrasonicated again, since the extract mainly contains large molecular weight DNA. This DNA extract was applied to characterize one “diarrheal” and one “emetic” strain of B. cereus with the DNA chip arrays. The results agreed with PCR control analysis which means that these electric DNA chip arrays can be used to characterize bacterial colonies with respect to the genes coding of all known toxins of B. cereus: haemolysin (hblA, hblC, hblD), non-haemolytic enterotoxin (nheA, nheB, nheC), cytotoxin K-2 (cytK-2), and cereulide (ces). The chip assay required about 30 min after application of DNA samples. Due to the generic properties of the chips, this technique should also be applicable for characterization of the pathogenicity potential of many other organisms. Keywords: Bacillus cereus, haemolysin, non-haemolytic enterotoxin, cytotoxin K-2, cereulide, toxin-coding genes, bacterial colony, electric DNA chip, ultrasonication, DNA fragmentation. / QC 20101111

No description available.