Tato disertační práce zavádí jednostranné gramatiky s nahodilým kontextem jako řízené gramatiky založené na bezkontextových gramatikách. V těchto gramatikách je ke každému pravidlu přiřazena množina povolujících symbolů a množina zakazujících symbolů a množina pravidel je rozdělena na množinu levých pravidel s nahodilým kontextem a množinu pravých pravidel s nahodilým kontextem . Levým pravidlem s nahodilým kontextem lze přepsat neterminál pokud se všechny povolující symboly vyskytují vlevo od přepisovaného neterminálu a žádný zakazující symbol tam přítomen není. Pravé pravidlo s nahodilým kontextem lze aplikovat analogicky, ale ona kontrola na přítomnost a nepřítomnost symbolů je provedena doprava od přepisovaného neterminálu. Práce je rozdělena na tři části. První část uvádí motivaci za zavedením jednostranných gramatik s nahodilým kontextem a umisťuje materiál pokrytý v této práci do vědeckého kontextu. Poté dává přehled základů teorie formálních jazyků a některých méně známých oblastí, jejichž znalost je nutná pro pochopení studovaného tématu. Druhá část tvoří jádro práce. Formálně definuje jednostranné gramatiky s nahodilým kontextem a studuje je z mnoha pohledů. Mezi studovaná témata patří generativní síla, vztah k jiným typům gramatik, redukce, normální formy, nejlevější derivace, zobecněné a LL verze těchto gramatiky. Třetí část této práce zakončuje diskusi několika poznámkami. Mezi ně patří poznámky týkající se aplikovatelnosti zavedených gramatik v praxi, bibliografie a otevřených problémů.

This master's thesis deals with implementation of ISO C99 language interpreter. The goal of this thesis is to provide support of education in C language programming and fast algorithm prototyping. It enables students to create own C programs and to experiment with language constructions without compiling. User interface includes editor and simple debugger. The interpreter is implemented in a novel grammar development environment written in Java language - ANTLRWorks which includes ANTLR language tool.

23000 tonnes de déchets graphites seront générés lors du démantèlement de la première filière de réacteurs en France (9 réacteurs Uranium Naturel Graphite Gas, UNGG). Ces déchets radioactifs sont classés dans la catégorie Faible Activité Vie Longue (FAVL). Dans le cadre de la loi, l’agence nationale pour la gestion des déchets radioactifs (Andra) étudie un concept de stockage à faible profondeur. Cette étude s’intéresse plus particulièrement au carbone 14, qui est un des principaux radionucléides à vie longue (5730 ans) dans les déchets graphite, mais aussi au tritium qui est l’un des principaux contributeurs de la radioactivité à court terme. Ces deux radionucléides ont la particularité d’exister sous différentes formes, aussi bien en phase gaz (14CO2, HT,…) qu’en phase liquide (14CO32-, HTO,…). Leur spéciation va influencer leur migration du stockage vers l’environnement. Des expériences de lixiviation en milieu alcalin (NaOH 0,1mol.L-1, simulant les conditions de stockage), ont été réalisées sur des échantillons de graphites irradiés provenant de deux réacteurs : SLA2 et G2, afin de quantifier leur relâchement et de définir leur spéciation. Les études montrent que le carbone se trouve aussi bien en phase gaz qu’en phase liquide. Dans la phase gaz, le relâchement est faible (< 0,1%), et correspond à des formes oxydables. Le carbone 14 est relâché majoritairement en phase liquide : 65% de la fraction d’inventaire relâchée est sous forme de carbone 14 inorganique, et 35% de carbone 14 organique. Deux formes de tritium ont été identifiées dans la phase gaz : HTO et HT/Tritium Organiquement Lié. Plus de 90% du tritium en phase gaz se trouve sous forme HT/TOL, mais ce relâchement est faible (<0,1%). Majoritairement le tritium est en phase liquide sous forme HTO. / 23000 tons of graphite wastes will be generated during dismantling of the first generation of French reactors (9 gas cooled reactors). These wastes are classified as Long Lived Low Level wastes (LLW-LL). As requested by the law, the French National Radioactive Waste Management Agency (Andra) is studying concepts of low-depth disposals.In this work we focus on carbon 14, the main long-lived radionuclide in graphite waste (5730y), but also on tritium, which is the main contributor to the radioactivity in the short term. Carbon 14 and tritium may be released from graphite waste in many forms in gaseous phase (14CO2, HT…) or in solution (14CO32-, HTO…). Their speciation will strongly affect their migration from the disposal site to the environment. Leaching experiments, in alkaline solution (0.1 M NaOH simulating repository conditions) have been performed on irradiated graphite, from Saint-Laurent A2 and G2 reactors, in order to quantify their release and characterize their speciation. The studies show that carbon 14 exists in both gaseous and aqueous phases. In the gaseous phase, release is weak (<0.1%) and corresponds to oxidizable species. Carbon 14 is mainly released into liquid phase, as both inorganic and organic species. 65% of released fraction is inorganic and 35% organic carbon. Two tritiated species have been identified in gaseous phase: HTO and HT/Organically Bond Tritium. More than 90% of tritium in that phase corresponds to HT/OBT. But release is weak (<0.1%). HTO is mainly in the liquid phase.

Neste trabalho, foram estudadas as propriedades fotoquímica e/ou fotofísica de alguns compostos de coordenação de rênio(l) e ferro(I I). A irradiação dos complexos fac-[Re(CO)3(NN)(trans-L)]+, NN= 4,7-difenil-1,10- fenantrolina (ph2phen) ou 5-cloro-1,10-fenantrolina (Clphen) e L = 1,2-bis(4-piridil)etileno (bpe) ou 4-estirilpiridina (stpy), em acetonitrila ou em filme de poli(metacrilato de metila) (PMMA) resulta em variações espectrais condizentes com a fotoisomerização trans-cis do ligante coordenado. A determinação dos rendimentos quânticos para a fotorreação pela variação espectral resultou em valores aparentes, uma vez que o reagente e o fotoproduto absorvem na mesma região. Para a determinação do rendimento quântico real, &#934;real, utilizou-se a técnica de 1H RMN, na qual os sinais do fotoproduto e do reagente são observados em regiões distintas com diferentes constantes de acoplamento. Os valores de &#934;real obtidos para fac-[Re(CO)3h(NN)(trans-bpe)]+ (ph2phen: &#934;313= 0,43 ± 0,03; &#934;365= 0,44 ± 0,02; &#934;404= 0,43 ± 0,02; Clphen: &#934;313= 0,56 ± 0,03; &#934;365= 0,55 ± 0,04; &#934;404= 0,57 ± 0,06) são independentes do comprimento de onda de irradiação, indicando a existência de um único canal para a população do estado excitado 3ILtrans-bpe. Por outro lado, para fac-[Re(CO)3(NN)(trans-stpy)]+, os valores de &#934;real sob irradiação a 404 nm são menores que os determinados para os demais comprimentos de onda de irradiação (ph2phen: &#934;313= 0,60 ± 0,05; &#934;365= 0,64 ± 0,09; &#934;404= 0,42 ± 0,03; Clphen: &#934;313= 0,52 ± 0,05; &#934;365= 0,58 ± 0,02; &#934;404= 0,41 ± 0,06), indicando que, a energias maiores, em que o Iigante absorve significativamente, deve existir a contribuição de outro canal para a população do estado excitado 3ILtrans-stpy. A eficiência do fotoprocesso foi avaliada por meio da substituição dos ligantes NN e/ou L, e a diferença nos valores de &#934;real entre os complexos deve estar relacionada principalmente com as distintas eficiências de cruzamento intersistemas. o fotoprocesso altera as propriedades fotofísicas desses complexos. Os isômeros trans apresentam fraca ou nenhuma emissão a 298 K, enquanto os fotoprodutos, fac-[Re(CO)3(NN)(cis-L)]+, apresentam intensa luminescência dominada pelo estado excitado 3MLCTRe&#8594;NN, que é sensivel à rigidez do meio. A reatividade fotoquímica dos pentacianoferratos(II) [Fe(CN)5 (NN)]3-, NN= 2aminobenzilamina (aba), 2-aminobenzamida (ab), 2-(dimetilaminometil)-3-hidroxipiridina (dmampy), 2-aminometilpiridina (ampy), 2-aminoetilpiridina (aepy) ou 2-(2metilaminoetil) piridina (maepy), também foi investigada. A irradiação desses complexos resulta na fotossubstituição do CN-, a qual só pode ser detectada quando o ligante possui um segundo grupo coordenante nas proximidades da esfera de coordenação. Os rendimentos quânticos da fotossubstituição são dependentes do comprimento de onda de irradiação (&#934;313= 0,13 ± 0,01; &#934;334= 0,091 ± 0,001; &#934;365= 0,056 ± 0,002; &#934;404= 0,022 ± 0,002; &#934;436= 0,015 ± 0,001, por exemplo, para NN = aba) e indicam a existência de canais distintos pelos quais a fotorreação ocorre ou as diferentes eficiências de cruzamento intersistema para a população do estado excitado reativo. A eficiência do fotoprocesso também depende do Iigante utilizado (&#955;irr= 365 nm: &#934;aba= 0,056, &#934;ab= 0,14, &#934;ampy= 0,046, &#934;aepy= 0,066, &#934;maepy= 0,069 e &#934;dmampy= 0,12). Na série das diaminas, o rendimento quântico é maior para [Fe(CN)5(ab)]3-, que possui dois sítios para ocorrer o fechamento do anel. Na série das aminopiridinas, observa-se a influência do comprimento da cadeia na eficiência do fechamento do anel. A presença de metilas ligadas ao nitrogênio alifático deve ter pouca ou nenhuma influência na eficiência do fotoprocesso. / The photochemical and/or photophysical properties of some coordination compounds of rhenium(l) and iron(II) were investigated. Photolyses of the fac-[Re(CO)3(NN)(trans-L)+ complexes, NN = 4,7-diphenyl-1, 10- phenanthroline (ph2phen) or 5-chloro-1,10-phenanthroline (Clphen), and L = 1,2-bis(4pyridyl) ethylene (bpe) or 4-styrylpyridine (stpy) in acetonitrile or in poly(methylmethacrilate) (PMMA) films led to spectral changes due to the trans-cis photoisomerization process of the coordinated ligand. Determination of quantum yields for the photoreaction from absorption spectral changes resulted in apparent values once the reactant and the photoproduct absorb in the same region. For the true quantum yields determination, &#934;true, the 1H NMR spectroscopy was employed since the signals of the photoproduct and the reactant are observed in distinct regions with different coupling constants. The &#934;true determined for fac-[Re(CO)3(NN)(trans-bpe)+ (ph2phen: &#934;313= 0.43 ± 0.03; &#934;365= 0.44 ± 0.02; &#934;404= 0.43 ± 0.02; Clphen: &#934;313= 0.56 ± 0.03; &#934;365= 0.55 ± 0.04; &#404= 0.57 ± 0.06) are independent on the irradiation wavelength indicating only one pathway for the 3ILtrans-bpe excited state population. On the other hand, for fac-[Re(CO)3(NN)(trans-stpy)+ the &#934;true under 404 nm irradiation are lower than those determined for the others irradiation wavelengths (ph2phen: &#934;313= 0.58 ± 0.04; &#934;365= 0.64 ± 0.09; &#934;404 =0.42 ± 0.04; Clphen: &#934;313= 0.54 ± 0.02; &#934;365= 0.58 ± 0.03; &#934;404= 0.42 ± 0.06) which indicate that at higher energies, where the ligand absorbs significantly, there is another pathway that contributes to the 3ILtrans-stpy excited state population. The photoprocess efficiency was evaluated by changing the NN and/or L Iigands, and the differences between the &#934;true can be rationalized mainly in terms of distinct intersystem crossing efficiencies.The photoprocess changes the photophysical properties of these complexes. While the trans isomers exhibit week or no emission at 298 K, the photoproducts, fac-[Re(CO)3(NN)(cis-L)]+, exhibit intense emission driven by 3MLCTRe&#8594;NN excited state, which is sensitive to the medium rigidity. The photochemical reactivity of pentacyanoferrate(II) complexes [Fe(CN)5(NN)]3-, NN = 2-aminobenzilamine (aba), 2-aminobenzamide (ab), 2-(dimethylaminomethyl)-3hydroxypyridine (dmampy), 2-amonomethylpyridine (ampy), 2-aminoethylpyridine (aepy), or 2-(2-methylaminoethyl)pyridine (maepy), were also investigated. Irradiation of these complexes results in the CN- photosubstitution which is detected only when the ligand has an additional coordinating group available near the coordination sphere. The photosubstitution quantum yields are dependent on the irradiation wavelength (&#934;313= 0.13 ± 0.01; &#934;334= 0.091 ± 0.001; &#934;365= 0.056 ± 0.002; &#934;404= 0.022 ± 0.002; &#934;436= 0.015 ± 0.001 for NN = aba), indicating the existence of distinct pathways in which the photoreaction takes place or the distinct population of the reactive excited state due to different intersystem crossing efficiencies. The efficiency of the photoprocess is also dependent on the Iigand (&#955;Irr= 365 nm: &#934;aba= 0.056, &#934;ab= 0.14, &#934;ampy= 0.046, &#934;aepy= 0.066, &#934;maepy= 0.069 e &#934;dmampy= 0.12). For the diamines complexes, the higher quantum yield is observed for [Fe(CN)5(ab)]3-, which exhibit two sites to occur the ring closure. For the aminopiridines, it is óbserved the importance of the chain length on the closure ring efficiency. And that the presence of methyl groups attached to the nitrogen has week or no influence on the photoprocess efficiency.

Neste trabalho, foram estudadas as propriedades fotoquímica e/ou fotofísica de alguns compostos de coordenação de rênio(l) e ferro(I I). A irradiação dos complexos fac-[Re(CO)3(NN)(trans-L)]+, NN= 4,7-difenil-1,10- fenantrolina (ph2phen) ou 5-cloro-1,10-fenantrolina (Clphen) e L = 1,2-bis(4-piridil)etileno (bpe) ou 4-estirilpiridina (stpy), em acetonitrila ou em filme de poli(metacrilato de metila) (PMMA) resulta em variações espectrais condizentes com a fotoisomerização trans-cis do ligante coordenado. A determinação dos rendimentos quânticos para a fotorreação pela variação espectral resultou em valores aparentes, uma vez que o reagente e o fotoproduto absorvem na mesma região. Para a determinação do rendimento quântico real, &#934;real, utilizou-se a técnica de 1H RMN, na qual os sinais do fotoproduto e do reagente são observados em regiões distintas com diferentes constantes de acoplamento. Os valores de &#934;real obtidos para fac-[Re(CO)3h(NN)(trans-bpe)]+ (ph2phen: &#934;313= 0,43 ± 0,03; &#934;365= 0,44 ± 0,02; &#934;404= 0,43 ± 0,02; Clphen: &#934;313= 0,56 ± 0,03; &#934;365= 0,55 ± 0,04; &#934;404= 0,57 ± 0,06) são independentes do comprimento de onda de irradiação, indicando a existência de um único canal para a população do estado excitado 3ILtrans-bpe. Por outro lado, para fac-[Re(CO)3(NN)(trans-stpy)]+, os valores de &#934;real sob irradiação a 404 nm são menores que os determinados para os demais comprimentos de onda de irradiação (ph2phen: &#934;313= 0,60 ± 0,05; &#934;365= 0,64 ± 0,09; &#934;404= 0,42 ± 0,03; Clphen: &#934;313= 0,52 ± 0,05; &#934;365= 0,58 ± 0,02; &#934;404= 0,41 ± 0,06), indicando que, a energias maiores, em que o Iigante absorve significativamente, deve existir a contribuição de outro canal para a população do estado excitado 3ILtrans-stpy. A eficiência do fotoprocesso foi avaliada por meio da substituição dos ligantes NN e/ou L, e a diferença nos valores de &#934;real entre os complexos deve estar relacionada principalmente com as distintas eficiências de cruzamento intersistemas. o fotoprocesso altera as propriedades fotofísicas desses complexos. Os isômeros trans apresentam fraca ou nenhuma emissão a 298 K, enquanto os fotoprodutos, fac-[Re(CO)3(NN)(cis-L)]+, apresentam intensa luminescência dominada pelo estado excitado 3MLCTRe&#8594;NN, que é sensivel à rigidez do meio. A reatividade fotoquímica dos pentacianoferratos(II) [Fe(CN)5 (NN)]3-, NN= 2aminobenzilamina (aba), 2-aminobenzamida (ab), 2-(dimetilaminometil)-3-hidroxipiridina (dmampy), 2-aminometilpiridina (ampy), 2-aminoetilpiridina (aepy) ou 2-(2metilaminoetil) piridina (maepy), também foi investigada. A irradiação desses complexos resulta na fotossubstituição do CN-, a qual só pode ser detectada quando o ligante possui um segundo grupo coordenante nas proximidades da esfera de coordenação. Os rendimentos quânticos da fotossubstituição são dependentes do comprimento de onda de irradiação (&#934;313= 0,13 ± 0,01; &#934;334= 0,091 ± 0,001; &#934;365= 0,056 ± 0,002; &#934;404= 0,022 ± 0,002; &#934;436= 0,015 ± 0,001, por exemplo, para NN = aba) e indicam a existência de canais distintos pelos quais a fotorreação ocorre ou as diferentes eficiências de cruzamento intersistema para a população do estado excitado reativo. A eficiência do fotoprocesso também depende do Iigante utilizado (&#955;irr= 365 nm: &#934;aba= 0,056, &#934;ab= 0,14, &#934;ampy= 0,046, &#934;aepy= 0,066, &#934;maepy= 0,069 e &#934;dmampy= 0,12). Na série das diaminas, o rendimento quântico é maior para [Fe(CN)5(ab)]3-, que possui dois sítios para ocorrer o fechamento do anel. Na série das aminopiridinas, observa-se a influência do comprimento da cadeia na eficiência do fechamento do anel. A presença de metilas ligadas ao nitrogênio alifático deve ter pouca ou nenhuma influência na eficiência do fotoprocesso. / The photochemical and/or photophysical properties of some coordination compounds of rhenium(l) and iron(II) were investigated. Photolyses of the fac-[Re(CO)3(NN)(trans-L)+ complexes, NN = 4,7-diphenyl-1, 10- phenanthroline (ph2phen) or 5-chloro-1,10-phenanthroline (Clphen), and L = 1,2-bis(4pyridyl) ethylene (bpe) or 4-styrylpyridine (stpy) in acetonitrile or in poly(methylmethacrilate) (PMMA) films led to spectral changes due to the trans-cis photoisomerization process of the coordinated ligand. Determination of quantum yields for the photoreaction from absorption spectral changes resulted in apparent values once the reactant and the photoproduct absorb in the same region. For the true quantum yields determination, &#934;true, the 1H NMR spectroscopy was employed since the signals of the photoproduct and the reactant are observed in distinct regions with different coupling constants. The &#934;true determined for fac-[Re(CO)3(NN)(trans-bpe)+ (ph2phen: &#934;313= 0.43 ± 0.03; &#934;365= 0.44 ± 0.02; &#934;404= 0.43 ± 0.02; Clphen: &#934;313= 0.56 ± 0.03; &#934;365= 0.55 ± 0.04; &#404= 0.57 ± 0.06) are independent on the irradiation wavelength indicating only one pathway for the 3ILtrans-bpe excited state population. On the other hand, for fac-[Re(CO)3(NN)(trans-stpy)+ the &#934;true under 404 nm irradiation are lower than those determined for the others irradiation wavelengths (ph2phen: &#934;313= 0.58 ± 0.04; &#934;365= 0.64 ± 0.09; &#934;404 =0.42 ± 0.04; Clphen: &#934;313= 0.54 ± 0.02; &#934;365= 0.58 ± 0.03; &#934;404= 0.42 ± 0.06) which indicate that at higher energies, where the ligand absorbs significantly, there is another pathway that contributes to the 3ILtrans-stpy excited state population. The photoprocess efficiency was evaluated by changing the NN and/or L Iigands, and the differences between the &#934;true can be rationalized mainly in terms of distinct intersystem crossing efficiencies.The photoprocess changes the photophysical properties of these complexes. While the trans isomers exhibit week or no emission at 298 K, the photoproducts, fac-[Re(CO)3(NN)(cis-L)]+, exhibit intense emission driven by 3MLCTRe&#8594;NN excited state, which is sensitive to the medium rigidity. The photochemical reactivity of pentacyanoferrate(II) complexes [Fe(CN)5(NN)]3-, NN = 2-aminobenzilamine (aba), 2-aminobenzamide (ab), 2-(dimethylaminomethyl)-3hydroxypyridine (dmampy), 2-amonomethylpyridine (ampy), 2-aminoethylpyridine (aepy), or 2-(2-methylaminoethyl)pyridine (maepy), were also investigated. Irradiation of these complexes results in the CN- photosubstitution which is detected only when the ligand has an additional coordinating group available near the coordination sphere. The photosubstitution quantum yields are dependent on the irradiation wavelength (&#934;313= 0.13 ± 0.01; &#934;334= 0.091 ± 0.001; &#934;365= 0.056 ± 0.002; &#934;404= 0.022 ± 0.002; &#934;436= 0.015 ± 0.001 for NN = aba), indicating the existence of distinct pathways in which the photoreaction takes place or the distinct population of the reactive excited state due to different intersystem crossing efficiencies. The efficiency of the photoprocess is also dependent on the Iigand (&#955;Irr= 365 nm: &#934;aba= 0.056, &#934;ab= 0.14, &#934;ampy= 0.046, &#934;aepy= 0.066, &#934;maepy= 0.069 e &#934;dmampy= 0.12). For the diamines complexes, the higher quantum yield is observed for [Fe(CN)5(ab)]3-, which exhibit two sites to occur the ring closure. For the aminopiridines, it is óbserved the importance of the chain length on the closure ring efficiency. And that the presence of methyl groups attached to the nitrogen has week or no influence on the photoprocess efficiency.

Ce travail propose une méthodologie pour l'analyse du fonctionnement global d'un système HPP ainsi qu'une étude théorique et expérimentale des systèmes commutateur à plasma (POS), schéma LL et Compression de Flux. Une modélisation analytique des phases de fonctionnement est donnée pour évaluer leur rendement et les comparer. En s'appuyant sur un développement de la MHD Hall et sur un modèle de distribution du plasma, l'étude POS expose les modes de pénétration de B pendant la conduction et la transition vers la physique de l'ouverture. L'analyse des essais LL montre des spécificités (configuration de B, pertes) et conduit à un modèle électrique complet. L'usage du modèle analytique pour l'étude des essais CF révèle la circulation du courant dans l'épaisseur du plasma et son impact sur l'efficacité du schéma. Enfin, les performances des futures machines sont traduites en caractéristiques spécifiques aux schémas et comparées à l'expérience pour aborder leur viabilité et les travaux à venir.

XML has emerged as leading document format for exchanging data. Because of vast amounts of XML documents available and transfered, there is a strong need to store and query information in these documents. However, the most companies are still using a RDBMS for their data warehouses and it is often necessary to combine legacy data with the ones in XML format, so it might be useful to consider storage possibilities for XML documents in a relation database. In this thesis we focused on structured and semi-structured data-based XML documents, because they are the most common when exchanging data and they can be easily validated against an XML schema. We propose a slightly modified Hybrid algorithm to shred doc- uments into relations using an XSD scheme and we allowed redundancy to make queries faster. Our goal was not to provide an academic solution, but fully working system supporting latest standards, which will beat up native XML databases both by performance and vertical scalability.

Indiana University-Purdue University Indianapolis (IUPUI) / Haemophilus ducreyi is the causative agent of the genital ulcer disease chancroid, which has been shown to facilitate the transmission of HIV. H. ducreyi is likely exposed to multiple sources of antimicrobial peptides in vivo. APs are small, cationic molecules with both bactericidal and immunomodulatory functions. Because H. ducreyi is able to establish and maintain an infection in an environment rich with antimicrobial peptides, we hypothesized that the bacterium was resistant to the bactericidal effects of these peptides. Using a 96-well AP bactericidal assay, we examined H. ducreyi susceptibility to eight human APs likely to be encountered at the site of infection, including the α-defensins human neutrophil peptide-1, human neutrophil peptide-2, human neutrophil peptide-3, and human defensin 5, the β-defensins human β defensin-2, human beta defensin-3, and human beta defensin-4, and the human cathelicidin, LL-37. H. ducreyi survival was compared to the survival of Escherichia coli ML35, a strain known to be susceptible to several antimicrobial peptides. H. ducreyi was significantly more resistant than E. coli ML35 to the bactericidal effects of all peptides tested. Furthermore, we found that representative class I and class II strains of H. ducreyi were each resistant to APs of each functional category, indicating that resistance to antimicrobial peptides could represent a conserved method of pathogenesis for H. ducreyi as a species. The H. ducreyi genome contains a homolog for the Sap influx transporter. To study the role of the H. ducreyi Sap transporter in AP resistance, we generated an isogenic sapA mutant and used the 96-well AP bactericidal assay to compare the AP susceptibility profiles of wild-type H. ducreyi, the sapA mutant and the sapA trans-complement to α-defensins, β-defensins, and LL-37. We observed a 25% decrease in the survival of the sapA mutant when it was exposed to LL-37. These findings suggest that the H. ducreyi Sap transporter plays a role in H. ducreyi resistance to LL-37, but it is likely that other AP resistance mechanisms co-exist within the bacterium.