Cardiovascular disease is the leading cause of death in the United States, and coronary artery disease (CAD) kills over 370,000 people annually. There are available therapies that offer a temporary solution; however, only a heart transplant can fully resolve heart failure, and donor organ shortages severely limit this therapy. C-kit+ human cardiac stem cells (hCSCs) offers a viable alternative therapy to treat cardiovascular disease by replacing damaged cardiac tissue; however, low cell viability, low retention/engraftment, and uncontrollable in vivo differentiation after transplantation has limited the efficacy of stem cell therapy. Tissue engineering solutions offer potential tools to overcome current limitations of stem cell therapy. Materials derived from natural sources such as keratin from human hair offers innate cellular compatibility, bioactivity, and low immunogenicity. Keratin proteins extracted using oxidative chemistry known as keratose (KOS) have shown therapeutic potential in a wide range of applications including cardiac regeneration. My studies utilize KOS hydrogels to modulate c-kit+ hCSC differentiation, and explore the capability of differentiated cells to regenerate vascular tissue. In the first Chapter, we reviewed literature relevant to keratin-based biomaterials and their biomedical applications, the use of stem cells in cardiovascular research, and the differentiation of vascular smooth muscle cells (VSMCs). The section on biomedical applications of keratin biomaterials focuses on the oxidized form of keratin known as keratose (KOS), because this was the material used for our research. Since we planned to use this material in conjunction with c-kit+ hCSCs, we also briefly explored the use of stem cells in cardiovascular research. Additionally, we examined some key signaling pathways, developmental origins, and the cell phenotype of VSMCs for reasons that will become clear after observing results from chapters 2 and 3. Based upon our review of the literature, KOS biomaterials and c-kit+ hCSCs were determined to be promising as a combined therapeutic for the regeneration of cardiac tissue. Research in Chapter 2 focused on characterizing the effects of KOS hydrogel on c-kit+ hCSC cell viability, proliferation, morphology, and differentiation. Results demonstrated that KOS hydrogels could maintain hCSC viability without any observable toxic effects, but it modulated cell size, proliferation, and differentiation compared to standard tissue culture polystyrene cell culture (TCPS). KOS hydrogel produced gene and protein expression consistent with a VSMC phenotype. Further, we also observed novel "endothelial cell tube-like" microstructures formed by differentiated VSMCs only on KOS hydrogel, suggesting a potential capability of the hCSC-derived VSMCs for in vitro angiogenesis. Results from this study lead us to question what signaling pathways might be responsible for the apparent VSMC differentiation pattern we observed on KOS hydrogels. Research in Chapter 3 explored the time course of VSMC differentiation, cell contractility, inhibition of VSMC differentiation, and measured protein expression of transforming growth factor beta 1 (TGF-β1) and its associated peptides for hCSCs cultured on KOS hydrogels, tissue culture polystyrene, and collagen hydrogels. A review of VSMC differentiation signaling pathways informed our decision to investigate the role of TGF-β1 in VSMC differentiation. Results demonstrated that KOS hydrogel differentiated hCSCs significantly increased expression for all three vascular smooth muscle (VSM) markers compared to TCPS differentiated cells. Additionally, KOS differentiated hCSCs were significantly more contractile than cells differentiated on TCPS. Recombinant human (rh) TGF-β1 was able to induce VSM differentiation on TCPS. VSM differentiation was successfully inhibited using TGF-β NABs and A83-01. Enzyme-Linked Immunosorbent Assay (ELISA) analysis revealed that both TCPS and KOS hydrogel differentiated cells produced TGF-β1, with higher levels being measured at early time points on TCPS and later time points on KOS hydrogels. Results from supplementing rhTGF-β1 to TCPS and KOS hydrogels revealed that KOS seems to interact with TGF-β to a greater extent than TCPS. Western blot results revealed that latency TGFβ binding protein (LTBP-1) and latency associated peptide (LAP) had elevated levels early during differentiation. Further, the levels of LTBP-1 and LAP were higher on KOS differentiated hCSCs than TCPS hCSCs. This study reaffirms previous results of a VSM phenotype observed on KOS hydrogels, and provides convincing evidence for TGF-β1 inducing VSM differentiation on KOS hydrogels. Additionally, results from ELISA and western blot provide evidence that KOS plays a direct role in this pathway via interactions with TGF-β]1 and its associated proteins LTBP-1 and LAP. Results from chapter 2 and 3 offered significant evidence that our cells exhibited a VSMC phenotype, and that TGF-β1 signaling was a key contributor for the observed phenotype, but we still needed an animal model to explore the therapeutic potential of our putative VSMCs. Research in Chapter 4 investigated a disease model to test the ability of KOS hydrogel differentiated cells to regenerate vascular tissue. To measure vascular regenerative capability, we selected a murine model of critical limb ischemia (CLI). CLI was induced in 3 groups (n=15/group) of adult mixed gender NSG mice by excising the femoral artery and vein, and then treated the mice with either PBS (termed as PBS-treated), Cells differentiated on TCPS (termed as Cells from TCPS), or KOS hydrogel-derived VSMCs (termed as Cells from KOS). Blood perfusion of the hind limbs was measured immediately before and after surgery, then 14, and 28 days after surgery using Laser Doppler analysis. Tissue vascularization, cell engraftment, and skeletal muscle regeneration were measured using immunohistochemistry, 1,1'-Dioctadecyl3,3,3',3'-Tetramethylindocarbocyanine Perchlorate (DiL) vessel painting, and hematoxylin and eosin (HandE) pathohistological staining. During the 4-week period, both cell treatment groups showed significant increases in blood perfusion compared to the PBS-treated control, and at day 28 the Cells from KOS group had significantly better blood flow than the Cells from TCPS group. Additionally, the Cells from KOS group demonstrated a significant increase in the ratio of DiL positive vessels, capillary density, and a greater density of small diameter arterioles compared to the PBS-treated group. Further, both cell-treated groups had similar levels of engraftment into the host tissue. We conclude that Cells from KOS therapy increases blood perfusion in an NSG model of CLI, but does not lead to increased cell engraftment compared to other cell based therapies. Overall, the results from this dissertation demonstrated that KOS hydrogels produce VSMC differentiation from c-kit+ hCSCs mediated by TGF-β1 signaling, and that the differentiated cells are able to increase blood perfusion in a CLI model by increasing capillary density, suggesting enhanced angiogenesis. Future studies should explore potential protein-protein interactions between KOS, TGF-β1 and its associated proteins. Additionally, we should plan animal studies that examine the efficacy of our cells to regenerate cardiac tissue following an acute myocardial infarction (AMI). / PHD

In der vorliegenden Arbeit werden der Entwurf, die Technologie und die Parameteridentifikation von Silizium basierten mikroelektromechanischen Systemen (MEMS) mit piezoelektrischen Dünnschicht-Aluminiumnitrid (AlN) vorgestellt. Auf Basis des AlNs als elektromechanischer Wandler erfolgt die Fertigung eines MEMS Technologiedemonstrators für energiearme Inertialsensoren. Das AlN wird über einen reaktiven Sputterprozess auf einer Wachstumsschicht abgeschieden. Durch Parametervariation des reaktiven Sputterprozesses und der Wachstumsschicht werden die piezoelektrischen Eigenschaften des AlNs optimiert. Die Entwicklung einer Gesamttechnologie führt zu einer Integration des Dünnschicht-AlNs in Silizium-Mikromechaniken. Die Röntgenbeugung (XRD) ermöglicht die Kristallstruktur des AlNs zu qualifizieren. Darüber hinaus werden weitere Analysemethoden vorgestellt, die eine hoch genaue und reproduzierbare messtechnische Bestimmung der piezoelektrischen Koeffizienten aus mikromechanischen Messstrukturen ermöglichen. Die Determination der piezoelektrischen Koeffizienten des Dünnschicht-AlNs aus den Messstrukturen erfolgt mittels analytischen und FE Modellen sowie der Laser-Doppler-Vibrometrie (LDV). Der Fokus der Arbeit liegt hierbei auf der Identifikation der longitudinalen und transversalen piezoelektrischen Ladungskoeffizienten des AlNs. Als Technologiedemonstrator wird ein einachsiger Inertialsensor mit integriertem piezoelektrischen Dünnschicht-AlN vorgestellt. Das MEMS generiert aufgrund des piezoelektrischen Wandlers intrinsisch elektrische Ladungen bei Einwirkung einer mechanischen Energie. Dadurch ist keine elektrische Energiezufuhr für die Messung eines inertialen Ereignisses notwendig. Der vorgestellte Demonstrator wird hinsichtlich seiner Ladungs- und Spannungssensitivität optimiert. Zur theoretischen Beschreibung der Funktionsweise werden analytische, sowie FE und SPICE Modelle genutzt. Eine Charakterisierung des MEMS Bauelements erfolgt hinsichtlich der mechanischen und elektrischen Eigenschaften. / The thesis includes the design, the technology and the parameter identification of silicon-based microelectromechanical systems (MEMS) with piezoelectric thin film of aluminum nitride (AlN). A low-energy inertial sensor as technology demonstrator based on AlN as an electromechanical transducer a MEMS manufacturing process is shown. The AlN is deposited via a reactive sputtering on a growth layer. By varying parameters of the reactive sputtering and the growth layer of AlN, the piezoelectric properties can be optimized. The development of an overall technology results to an integration of the thin film AlNs in silicon micromechanics. X-ray diffraction (XRD) allows to qualify the crystal structure of AlN. Further methods are developed that enable a highly accurate and repeatable metrological determination of piezoelectric coefficients measurement structures. The determination of piezoelectric coefficients of the thin film AlN from the measurement structures is resulting from analytical methods and FE models and the laser Doppler vibrometry (LDV). The identification of the longitudinal and transverse piezoelectric charge coefficient of AlN is one main focus of this work. A uniaxial inertial sensor with an integrated piezoelectric thin film of AlN is presented as technology demonstrator. The piezoelectric transducer of the MEMS is generating electric charges intrinsically as reaction of mechanical stress. Thus, no electric power supply for the measurement of an inertial event is necessary. The presented demonstrator has been optimized with respect to its charge and voltage sensitivity. For a theoretical description analytical and FE and SPICE models are used. A characterization of the MEMS device is carried out with regard to the mechanical and electrical properties.

L’étude présentée dans cette thèse relève de la mécanique des fluides expérimentale et numérique appliquée aux écoulements pariétaux de refroidissement de chambres de combustion aéronautiques. En présence de phénomènes thermo-acoustiques, comme les instabilités de combustion, il est important d’évaluer si les capacités de l’écoulement pariétal à protéger les parois de chambre restent suffisantes. C’est ainsi que nous nous sommes intéressés aux écoulements de paroi multiperforée soumis à une excitation acoustique. Dans ce but, le banc d’essais MAVERIC a été amélioré grâce à l’installation d’un système qui permet de forcer acoustiquement l’écoulement transverse dans lequel les jets pariétaux débouchent. Nous avons pu alors mettre en évidence la forte sensibilité de ce type d’écoulements à l’excitation acoustique. Le bon accord entre les résultats expérimentaux et les simulations numériques aux grandes échelles (LES) effectuées est très encourageant dans le cas d’un forçage par onde stationnaire. Le forçage par onde progressive, étudié uniquement par simulations numériques, s’est révélé être capable de modifier significativement la topologie de l’écoulement. Enfin, à partir de l’outil numérique AVBP-AVTP qui permet le couplage de calculs fluide-solide, nous avons réalisé une étude de l’influence de la présence d’une excitation acoustique sur le comportement thermique de l’écoulement autour d’une paroi multiperforée de chambre de combustion. / This experimental and numerical study in the field of fluid mechanics deals with jets-in cross flow configurations that are relevant for the cooling of aero engine combustion chambers. Indeed, in presence of instabilities it is important to determine to which extent the film cooling is able to do its job of preserving the combustion chamber walls from the thermal load. The test facility MAVERIC has been upgraded in order to acoustically force the crossflow in which the jets are discharging. The strong sensitivity of the overall flow unsteady properties to the presence of the acoustic forcing has been clearly evidenced. The agreement between the experimental results and large-eddy simulations proved to be quite encouraging for a stationary acoustic wave whereas the case of a propagating acoustic wave investigated only numerically reveals also quite a significant change of the flow topology. In this context, the effect of the acoustic forcing on the wall thermal behavior has been analyzed thanks to the use of the fluid-solid coupled AVBP-AVTP solver.

Τα ολοκληρωμένα ηλιακά συστήματα συλλέκτη αποθήκης αποτελούνται από μία δεξαμενή αποθήκευσης, της οποίας τμήμα της επιφάνειας της χρησιμοποιείται σαν ηλιακός συλλέκτης. Συνήθως το ρευστό της αποθήκης είναι το νερό χρήσης. Στο υπό εξέταση σύστημα το νερό χρήσης θερμαίνεται έμμεσα, διερχόμενο μέσα από σωληνώσεις εναλλάκτη θερμότητας που τοποθετείται στο εσωτερικό της παραλληλεπίπεδης δεξαμενής. Για την εντατικοποίηση της μετάδοσης θερμότητας προς το νερό χρήσης, δημιουργείται ανάδευση του ρευστού του δοχείου μέσω κυκλοφορητή, ο οποίος τίθεται σε λειτουργία μόνο όταν υπάρχει ζήτηση ζεστού νερού. Προς αποφυγή παραμορφώσεων τοποθετούνται πτερύγια συγκράτησης που ενώνουν τις δύο μεγάλες επιφάνειες της δεξαμενής. Στην παρούσα διδακτορική διατριβή διερευνήθηκαν τα ροϊκά φαινόμενα στο εσωτερικό του ICS που έχει περιγραφεί προηγουμένως, με στόχο τη μεγιστοποίηση της θερμανταλλαγής μεταξύ των δύο κυκλωμάτων νερού. Για την παρατήρηση του ροϊκού πεδίου καθώς και την λήψη μετρήσεων ταχυτήτων κατασκευάστηκε πειραματική συσκευή με διαφανή τοιχώματα από Plexiglas. Ελήφθησαν μετρήσεις ταχυτήτων και διακυμάνσεων με χρήση συστήματος Laser Doppler διπλής ακτίνας. Για την οπτικοποίηση του ροϊκού πεδίου τοποθετήθηκαν σωματίδια πολυστερίνης στο εσωτερικό της συσκευής. Ελήφθησαν ψηφιακές φωτογραφίες και βιντεοσκοπήσεις του ροϊκού πεδίου. Για την υπολογιστική προσομοίωση χρησιμοποιήθηκε το εμπορικό λογισμικό FLUENT. Αναπτύχθηκε υπολογιστικό μοντέλο και επιλύθηκε με όλα τα διαθέσιμα μοντέλα τύρβης. Στη συνέχεια πραγματοποιήθηκε σειρά υπολογιστικών προσομοιώσεων, στις οποίες διερευνήθηκε η βέλτιστη θέση και το μέγεθος των στομίων ανακυκλοφορίας, η βέλτιστη διάταξη των πτερυγίων συγκράτησης και η βέλτιστη θέση του εναλλάκτη. Επιπλέον προσδιορίστηκε υπολογιστικά και πειραματικά ο χρόνος αποκατάστασης του ροϊκού πεδίου. Τέλος, τα αποτελέσματα συγκρίθηκαν και με πειραματικά αποτελέσματα άλλων εργασιών. Τα συμπεράσματα που εξάγονται έχουν ως ακολούθως: Το μοντέλο τύρβης standard k-ω δίνει τα πιο αξιόπιστα αποτελέσματα. Το υπολογιστικό μοντέλο θεωρείται πιστοποιημένο μετά από πειραματική επιβεβαίωση ταχυτήτων και θερμοκρασιών. Το στόμιο ανακυκλοφορίας δεν πρέπει να τοποθετείται κάθετα στις μεγάλες επιφάνειες της δεξαμενής, ενώ η διάμετρος του πρέπει να είναι 1/2" ή και μικρότερη. Ο χρόνος αποκατάστασης του ροϊκού πεδίου είναι περίπου 35 s. Τα πτερύγια συγκράτησης πρέπει να ακολουθούν τις ροικές γραμμές. Ως βέλτιστη θέση του εναλλάκτη θεωρείται όταν τοποθετείται σε επαφή με το τοίχωμα. / Integrated Collector Storage (ICS) solar systems use part of the hot water storage as collector, i.e. half of the storage surface is used as absorber. Usually, the storage medium serves also as the energy transfer medium (service hot water). In the examined ICS, the service water is heated indirectly, passing through a serpentine heat exchanger placed inside the tank. The heat transfer from the stored water to the service water is intensified by the agitation of the stored water. A simple solution is the recirculation of the stored water by a small pump, which is functioning whenever a request for hot water exists. Fins in suitable positions, connect the front and back surface of the ICS, to withstand the deformation due to pressures by the tank water. In the present PhD thesis, the flow phenomena inside the ICS previous mentioned, are investigated. The aim is the maximization of the heat transfer between the two water circuits. An experimental device was constructed by transparent Plexiglas, for flow visualization and velocity measurements. A dual beam Laser Doppler Velocimetry (LDV) system was used to measure velocities. Polystyrene particles were added in the comprised water, for the visualization of the path lines. Photographs and video films were also taken. The commercial code FLUENT is used for the Computational Fluid Dynamics (CFD) simulations. A CFD model is developed and solutions are obtained using all the available turbulence models. Three main factors that influence the performance are optimized: the position and size of the recirculation ports, the arrangement and size of the interconnecting fins and the heat exchanger placement. The settling time, i.e., the time required for the flow field to be fully developed, is computed both numerically and experimentally. The previous analysis leads to the following conclusions: The standard k–ω model is selected as the most appropriate. The model is validated, with good agreement, against experimental measurements of velocities and temperatures. The placement of the inlet recirculation port perpendicular to the main surfaces of the ICS should be avoided, while its diameter should be 1/2" or less. The settling time is computed about 35s. The interconnecting fins, of the two main ICS surfaces should follow the flow filed path lines. The optimal placement of the tube heat exchanger is in contact with the two major surfaces of the storage tank.

Les dispositifs d’isolation galvanique intégrés au sein des systèmes de commande d’interrupteurs de puissance doivent répondre à une demande accrue en performance, facilité d’intégration et efficacité énergétique. Les transducteurs ultrasonores capacitifs micro-usinés (cMUT : capacitive Micromachined Ultrasonic Transducer), capables d’émettre et de recevoir des ondes ultrasonores, semblent une alternative tout à fait nouvelle à la fonction d’isolation galvanique. Ces travaux de thèse ont pour objectif de démontrer la faisabilité d’un dispositif basé sur la technologie cMUT. Le principe de fonctionnement consiste à transmettre une information grâce à une communication par onde acoustique de volume entre deux réseaux de cMUT placés de part et d’autre d’un substrat. Nous focalisons, en premier lieu, ces travaux sur le processus de fabrication par micro-usinage de surface des cMUT ainsi que les techniques de réalisation des dispositifs en structure double face sur substrat de silicium. L’étude permet d’identifier le collage de substrat comme une solution de fabrication industrialisable. Suite à la réalisation des dispositifs, la caractérisation électro-mécanique des cMUT est une étapeessentielle à la validation de leur fonctionnalité en tant que dispositifs émetteurs. L’étude débute par uneévaluation des propriétés mécaniques du matériau constituant la membrane et qui impactent directementle comportement global des cMUT. Puis, la caractérisation du comportement statique et dynamique descMUT permet d’extraire les paramètres tels que la fréquence de résonance, la tension de collapse etl’efficacité électro-mécanique qui définissent le mode de pilotage d’un tel système.Finalement, la validation du concept de transmission et de détection d’ondes ultrasonores est réaliséegrâce à des mesures de vibrométrie laser Doppler. Les résultats apportent des éléments de réponse quantau mode de propagation des ondes et permettent d’identifier les topologies de meilleure efficacité entransmission acoustique. Enfin, l’intégration du prototype dans l’application de commanded’interrupteur de puissance démontre la faisabilité du concept de transformateur acoustique basé sur latechnologie cMUT. / Galvanic isolation devices integrated into switch command systems must be able to answer all of the increasing demand for performance, energetic efficiency and integration easiness. The capacitive micro machined ultrasonic transducers (cMUT), able to emit and receive ultrasounds, could be an entirely new alternative to the function of galvanic isolation. This work aims to demonstrate the feasibility of a cMUT-based device. The operating principle consists in transmitting information thanks to a bulk acoustic wave between two cMUT arrays located on both sides of a substrate. We first focus on cMUT surface micromachining fabrication process and techniques of double-side device manufacturing. Our study allows us to identify wafer bonding as a realistic industrial solution. After device fabrication, electro-mechanical of cMUT is an essential step to validate their functionality as ultrasonic emitters. The study starts with the mechanical properties evaluation of the membrane material. These properties directly impact the global behavior of cMUT. Then, the characterization of cMUT static and dynamic behavior allows extracting parameters as resonance frequency, collapsing voltage and electro-mechanical efficiency which define the actuation mode of such a system. Finally, the validation of transmission and reception of ultrasonic waves is evaluated by vibrometer laser Doppler measurements. Results bring elements concerning the waves propagation modes and allow identifying the best acoustical efficiency in regard to the topology. In conclusion, the prototype integration in the application of power switch command demonstrates the feasibility of acoustic transformer concept based on cMUT technology.

Afin d’alléger les moteurs d’avions et diminuer la consommation de carburant, les industriels tendent à rendre plus compact le système de compression de leurs moteurs, qui représente environ 40% de la masse totale. Or, à taux de compression global égal, la réduction du nombre d’étages implique une charge aérodynamique plus élevée par étage. Cela augmente d’autant les risques de décollements sur les aubes et la dégradation des performances. L’aspiration de la couche limite sur les aubages s’est révélée très prometteuse pour supprimer ces décollements néfastes et satisfaire aux besoins de charge aérodynamique élevée. Cependant, l’aspiration modifie fortement la distribution de pression statique à la paroi des aubes, rendant les approches de conception traditionnelles inadaptées. L’objectif de ce travail de thèse est donc de proposer une nouvelle méthode et de nouveaux critères de conception d’aubages fortement chargés, intégrant l’aspiration de la couche limite. Cette méthode repose sur une stratégie d’aspiration en deux étapes. Dans un premier temps, un contrôle passif, par courbure et diffusion, de la position du point de décollement est effectué dans le but de la rendre insensible aux conditions de fonctionnement. Dans un second temps, un contrôle actif par aspiration vise à placer la fente d’aspiration par rapport au point de décollement de manière à minimiser le taux d’aspiration nécessaire au recollement de la couche limite. Afin de mettre en pratique cette stratégie, une technique de dessin d’aubages par prescription de la distribution de courbure de l’extrados et de la variation de section du canal inter-aubes, est ainsi développée. Associée à un outil de pré-dimensionnement rapide ainsi qu’une évaluation des pertes de pression totale incluant la présence d’aspiration, cette méthode permet ainsi de concevoir une grille de stator aspirée subsonique réalisant une déflexion fluide de 60 degrés, pour un nombre de Mach amont de 0,5, correspondant à un facteur de diffusion de 0,73. Cette performance au point nominal est obtenue avec un coefficient de pertes de pression totale de 2,5%, en aspirant 1,1% du débit entrant dans la grille. Ces valeurs peuvent néanmoins être réduites respectivement à 2,1% et 0,8% par l’emploi d’une fente d’aspiration à bords arrondis. Cette étude numérique bidimensionnelle est effectuée à l’aide du code de calcul elsA de l’ONERA. Afin de valider expérimentalement cette méthode de conception ainsi que les outils numériques associés, une grille d’aubes plane est construite et testée à basse vitesse au laboratoire de Mécanique de Fluides et d’Acoustique de l’Ecole Centrale de Lyon. A mi-envergure, les résultats issus de l’expérience et de simulations numériques 3D confirment la pertinence de la stratégie d’aspiration et la démarche de conception adoptée. Cette confrontation met alors en évidence l’impact de la distribution du taux d’aspiration suivant l’envergure sur l’efficacité de l’aspiration. Etant donné l’importance des écoulements tridimensionnels rencontrés, une généralisation en trois dimensions de la stratégie d’aspiration est proposée et est appliquée numériquement sur cette même grille d’aubes. En contrôlant simultanément les couches limites se développant sur l’aube et sur les parois latérales du canal de compression, il est alors possible de supprimer presque totalement les décollements de coins présents dans celui-ci. En contrepartie, le taux d’aspiration voit sa valeur augmenter très fortement, tempérant ce bénéfice. L’épaisseur des couches limites entrantes se révèle alors également être un facteur déterminant pour le succès du contrôle des couches limites par aspiration, dans un cadre tridimensionnel. / In order to reduce the mass of aircraft jet engines as well as their fuel consumption, manufacturers tend to make the compression system of their engines more compact, since this component represents approximately 40% of the total mass. However, for a given overall pressure ratio, decreasing the number of stages implies increasing the aerodynamic load per stage. This all the more increases the risk of flow separation on the blades ultimately resulting in a decrease in performance. Boundary layer suction on the blade has proven to be very promising to suppress this deleterious flow separation and meet the needs of high aerodynamic loads. Nevertheless, boundary layer suction significantly modifies the static pressure distribution on the blades, making traditional design approaches unsuitable. Therefore, the objective of this Ph.D. work is to develop a new method and new criteria for the design of highly loaded compressor blades, integrating boundary layer suction into the design process. This design method relies on a two-step aspiration strategy. First, passive control of the separation point location is applied via curvature and diffusion in order to make it insensitive to operating conditions. Second, active control through boundary layer suction aims at placing the suction slot with respect to the separation point location, in order to minimize the necessary suction mass flow rate required to reattach the flow. To put this strategy into practice, a blading technique that consists of prescribing the curvature distribution on the suction side of the blade and the cross-section distribution of the blade passage is developed. In association with a fast pre-design tool, as well as an overall total pressure loss coefficient including aspiration, this method allows the design of a subsonic aspirated stator cascade with flow turning of 60 degrees, for an inlet Mach number of 0.5,giving a Diffusion Factor of 0.73. This performance at the design point is obtained for an overall total pressure loss coefficient of 2.5%, aspirating 1.1% of the inlet mass flow rate. Nevertheless, these two values can be respectively reduced to 2.1% and 0.8% by rounding the edges of the suction slot. This bi-dimensional numerical study has been carried out with the elsA solver from ONERA. To experimentally validate this design method and the associated numerical tools, a planar cascade is built and tested at low speed at the Laboratoire de Mécanique de Fluides et d’Acoustique at the Ecole Centrale de Lyon. At mid-span, results from the experiment and from tri-dimensional numerical simulations confirm the relevance of the design approach. This comparison then discloses the impact of the suction mass flow rate distribution along the span, on the efficiency of aspiration. Given the importance of tri-dimensional flows encountered in the experiment and simulations, a generalization in three dimensions of the aspiration strategy is proposed and numerically applied on the same cascade. By simultaneously controlling the boundary layers developing on the blades and on the endwalls,it is possible to almost entirely suppress the corner separations present in the blade passage. However, one disadvantage is that the suction mass flow rate undergoes a strong increase, moderating this benefit. The thickness of the inlet boundary layers appears to be also a key factor in the success of boundary layer control by aspiration, in a tri-dimensional context.

Les recherches sur les instationnarités des Interactions Ondes de Choc Couches Limites (IOCCL) turbulentes ont permis une description détaillée de celles-ci tant expérimentalement que numériquement . Ceci a conduit à plusieurs schémas susceptibles d'expliquer les respirations à basses fréquences observées dans de tels écoulements. Les configurations avec des conditions amont laminaires ou transitionnelles ont été moins étudiées.Dans le cadre du programme Européen TFAST, un important effort a été mené afin de développer des dispositifs expérimentaux, conjointement à des simulations numériques, permettant une étude détaillée de ces configurations. Dans le cadre de cette thèse, on a mis en place une configuration de réflexion d'onde de choc sur une couche limite laminaire pour un nombre de Mach de 1.68. L'utilisation des métrologies classiques (Anémométrie Laser Doppler, Anémométrie Fil Chaud), adaptées à ces conditions expérimentales particulières, a permis de décrire les propriétés spatio-temporelles de ces écoulements. Le champ moyen a été caractérisé et comparé aux théories classique et aux résultats obtenus dans différentes souffleries.Un schéma décrivant le mécanisme de transition à la turbulence au sein de l'interaction a été développé. Sa sensibilité aux conditions amont a été étudiée en plaçant des perturbations en amont de l'interaction. Dans tous les cas, des instationnarités convectives (haute fréquence) et stationnaires (basse fréquence) ont été observées et comparées à celles existantes pour les configurations amont turbulentes. Une gamme intermédiaire d'instationnarités convectives (moyenne fréquence) a été mise en évidence et caractérisée. / Research dedicated to the study of the unsteadiness of turbulent Shock Wave Boundary Layer Interaction (SWBLI) has allowed a detailed description of this kind of interaction both experimentally and numerically. Several scenario were proposed to explain the low frequency unsteadiness observed in separated SWBLI. Nevertheless, the literature on this kind of flow involving either upstream laminar or transitional conditions is quite reduce. Within the framework of the European TFAST program, an important effort was made to develop experimental devices, in conjunction with numerical simulations, allowing a detailed study of these laminar or transitional configurations. In particular, within the framework of this thesis, a shock wave reflection configuration on a laminar boundary layer was set-up, with a nominal free stream Mach number of 1.68. Using classical metrology (Laser Doppler Anemometry, Hot WireAnemometry) that have been adapted to these particular experimental conditions, we have been able to describe the spatio-temporal properties of the interaction. The mean field has been characterized and compared with the classical theories and the results obtained in other configurations.A model describing the transition mechanisms to turbulence within the interaction has been developed. Its sensitivity to upstream conditions was studied by placing perturbations upstream of the interaction. In all cases, convective (high frequency) and stationary (low frequency) unsteadiness were observed and compared with those existing for upstream turbulent configurations. An intermediate range of convective unsteadiness (medium frequency) has been demonstrated and characterized.

In der vorliegenden Arbeit werden der Entwurf, die Technologie und die Parameteridentifikation von Silizium basierten mikroelektromechanischen Systemen (MEMS) mit piezoelektrischen Dünnschicht-Aluminiumnitrid (AlN) vorgestellt. Auf Basis des AlNs als elektromechanischer Wandler erfolgt die Fertigung eines MEMS Technologiedemonstrators für energiearme Inertialsensoren. Das AlN wird über einen reaktiven Sputterprozess auf einer Wachstumsschicht abgeschieden. Durch Parametervariation des reaktiven Sputterprozesses und der Wachstumsschicht werden die piezoelektrischen Eigenschaften des AlNs optimiert. Die Entwicklung einer Gesamttechnologie führt zu einer Integration des Dünnschicht-AlNs in Silizium-Mikromechaniken. Die Röntgenbeugung (XRD) ermöglicht die Kristallstruktur des AlNs zu qualifizieren. Darüber hinaus werden weitere Analysemethoden vorgestellt, die eine hoch genaue und reproduzierbare messtechnische Bestimmung der piezoelektrischen Koeffizienten aus mikromechanischen Messstrukturen ermöglichen. Die Determination der piezoelektrischen Koeffizienten des Dünnschicht-AlNs aus den Messstrukturen erfolgt mittels analytischen und FE Modellen sowie der Laser-Doppler-Vibrometrie (LDV). Der Fokus der Arbeit liegt hierbei auf der Identifikation der longitudinalen und transversalen piezoelektrischen Ladungskoeffizienten des AlNs. Als Technologiedemonstrator wird ein einachsiger Inertialsensor mit integriertem piezoelektrischen Dünnschicht-AlN vorgestellt. Das MEMS generiert aufgrund des piezoelektrischen Wandlers intrinsisch elektrische Ladungen bei Einwirkung einer mechanischen Energie. Dadurch ist keine elektrische Energiezufuhr für die Messung eines inertialen Ereignisses notwendig. Der vorgestellte Demonstrator wird hinsichtlich seiner Ladungs- und Spannungssensitivität optimiert. Zur theoretischen Beschreibung der Funktionsweise werden analytische, sowie FE und SPICE Modelle genutzt. Eine Charakterisierung des MEMS Bauelements erfolgt hinsichtlich der mechanischen und elektrischen Eigenschaften. / The thesis includes the design, the technology and the parameter identification of silicon-based microelectromechanical systems (MEMS) with piezoelectric thin film of aluminum nitride (AlN). A low-energy inertial sensor as technology demonstrator based on AlN as an electromechanical transducer a MEMS manufacturing process is shown. The AlN is deposited via a reactive sputtering on a growth layer. By varying parameters of the reactive sputtering and the growth layer of AlN, the piezoelectric properties can be optimized. The development of an overall technology results to an integration of the thin film AlNs in silicon micromechanics. X-ray diffraction (XRD) allows to qualify the crystal structure of AlN. Further methods are developed that enable a highly accurate and repeatable metrological determination of piezoelectric coefficients measurement structures. The determination of piezoelectric coefficients of the thin film AlN from the measurement structures is resulting from analytical methods and FE models and the laser Doppler vibrometry (LDV). The identification of the longitudinal and transverse piezoelectric charge coefficient of AlN is one main focus of this work. A uniaxial inertial sensor with an integrated piezoelectric thin film of AlN is presented as technology demonstrator. The piezoelectric transducer of the MEMS is generating electric charges intrinsically as reaction of mechanical stress. Thus, no electric power supply for the measurement of an inertial event is necessary. The presented demonstrator has been optimized with respect to its charge and voltage sensitivity. For a theoretical description analytical and FE and SPICE models are used. A characterization of the MEMS device is carried out with regard to the mechanical and electrical properties.

In recent years, application of confluent jets for design of ventilation supply devices has been studied. Similarly, numerus studies have been made on the potential and application of variable air volume (VAV) in order to reduce the energy demand of ventilation systems. This study investigates the combination of supply devices based on confluent jets and VAV, both in terms of the nearfield flow behavior of the device and the impact on thermal comfort, indoor air quality and energy efficiency on a classroom-level space when the airflow rate is varied. The method used in this study is an experimental field study where the confluent jets-based supply devices were compared to the previously installed displacement ventilation. The field study evaluated the energy efficiency, thermal comfort and indoor air quality of the two systems. In the case of the confluent jets supply devices, airflow rate was varied in order to see what impact the variation had on the performance of the system for each airflow rate. Furthermore, the confluent jets supply devices were investigated both experimentally and numerically in a well insulated test room to get high resolution data on the particular flow characteristics for this type of supply device when the airflow rate is varied. The results from the field study show nearly uniform distribution of the local mean age of air in the occupied zone, even in the cases of relatively low airflow rates. The airflow rates have no significant effect on the degree of mixing. The thermal comfort in the classroom was increased when the airflow rate was adapted to the heat load compared to the displacement system. The results lead to the conclusion that the combination of supply devices based on confluent jets can reduce energy usage in the school while maintaining indoor air quality and increasing the thermal comfort in the occupied zone. The results from the experimental and numerical study show that the flow pattern and velocity in each nozzle is directly dependent on the total airflow rate. However, the flow pattern does not vary between the three different airflow rates. The numerical investigation shows that velocity profiles for each nozzle have the same pattern regardless of the airflow rate, but the magnitude of the velocity profile increases as the airflow increases. Thus, a supply device of this kind could be used for variable air volume and produce confluent jets for different airflow rates. The results from both studies show that the airflow rate does not affect the distribution of the airflow on both near-field and room level. The distribution of air is nearly uniform in the case of the near-field results and the room-level measurement shows a completely uniform degree of mixing and air quality in the occupied zone for each airflow rate. This means that there is potential for combining these two schemes for designing air distribution systems with high energy efficiency and high thermal comfort and indoor air quality. / Under senare tid har applikation av Confluent jets för design av tilluftsdon studerats. Många studier har även utförts över potentialen av att applicera variabelt luftflöde (VAV) för att minska energianvändningen i ventilationssystem. Denna studie undersöker möjligheten att kombinera Confluent jets-don med VAV, både med avseende på den lokala flödesbilden och dess påverkan på termisk komfort, luftkvalitet och energieffektivitet i en klassrumsmiljö där luftflödes varieras. Denna studie baseras dels på en experimentell fältstudie där tilluftsdon baserade på Confluents jets jämfördes med befintliga deplacerande tilluftsdon. Fältstudien utvärderade energieffektiviteten, den termiska komforten och luftkvaliteten för båda typerna av tillluftsdon. Confluent jets-donen testades under varierat luftflöde för att se påverkan av flödesvariationen på ventilationens prestation under de olika flödena. Utöver fältstudien testades Confluent jets-donen experimentellt och numeriskt i ett välisolerat test-rum för få den detaljerade flödeskarakteristiken för den här typen tilluftsdon vid varierat luftflöde. Resultaten från fältstudien visar på en jämn fördelning av den lokala luftsmedelåldern i vistelsezonen, även för fallen med relativt låga luftflöden. Luftflöden har ingen signifikant effekt på omblandningen. Den termiska komforten i klassrummet ökade när luftflödet anpassades efter värmelasten jämfört med de deplacerande donen. Slutsatsen från fältstudien är att kombinationen av VAV och Confluent jets-don kan användas för att minska energianvändningen på skolan och bevara luftkvaliteten och den termiska komforten i vistelsezonen. Resultaten från den experimental och numeriska studien visar luftflödet och lufthastigheten i varje enskild dysa är direkt beroende på det totala luftflödet genom donet. Dock är flödesfördelningen mellan dysorna oberoende av de tre olika luftflödena. Den numeriska undersökningen visar att flödesprofilen för varje dysa är konstant trots att flödet varieras, men amplituden för varje profil ökar med en höjning av luftflödet. Det betyder att tilluftsdon av den här typen kan användas med VAV för att producera Confluent jets för olika luftflöden. Resultaten från båda studierna visar att luftflöde inte påverkar fördelningen av luften vare sig längs luftdonen eller på rumsnivå. Fördelningen av luften är nästan helt jämn längs donen och på rumsnivå är omblandningen och luftkvalitet den samma för varje luftflöde. Det betyder att det finns potential för att kombinera det här två teknikerna för att designa luftdistribueringssystem med hög energieffektivitet och hög termisk komfort med god luftkvalitet.

[ES] En un presente marcado por la continua lucha contra la contaminación y el cambio climático, la investigación en mejoras tecnológicas que permitan una transición aceptable para la sociedad hacia un futuro más ecológico ocupa un papel fundamental. En concreto, la aviación es un foco constante de innovación, ya que es considerada una función indispensable en una sociedad tan globalizada como la actual, pero con unos niveles de contaminación preocupantes. En este aspecto, el desarrollo de motores con altas eficiencias es un paso clave para la transición medioambiental. Sin embargo, estas alternativas presentan un reto tecnológico en cuanto a su gestión térmica basado, principalmente, en la necesidad de aumentar la refrigeración. En este contexto e impulsada por el proyecto "Aerodynamic upgrade of Surface Air Cooled Oil Cooler (SACOC)" de Clean Sky 2, esta tesis doctoral se centra en el estudio experimental de geometrías de intercambiadores de calor de superficie para la refrigeración del aceite motor mediante el uso del aire del flujo secundario del turbofan. Actualmente, existen una serie de limitaciones en cuanto a las capacidades para llevar a cabo un análisis y diseño detallado de este tipo de intercambiadores de calor debido a la falta de instalaciones que permitan un correcta, completa y robusta caracterización experimental. Las principales fuentes de datos se basan en cálculos numéricos validados a partir de extrapolaciones en condiciones de cuestionable aplicabilidad. A lo largo de la tesis se presentan los resultados obtenidos tras una detallada caracterización de cuatro diferentes geometrías de intercambiadores de calor empleando tanto técnicas intrusivas como ópticas. Se utiliza un banco de flujo capaz de generar una corriente de aire típica alrededor de los intercambiadores, mientras que un sistema de acondicionamiento de aceite controla el punto de operación por el lado caliente. Para recrear de manera más realista las condiciones de funcionamiento, se presenta una metodología para generar de manera automática pantallas de distorsión que pueden reproducir una distribución bidimensional de velocidades objetivo mediante la manufactura aditiva de paneles de porosidad variable. Este modelo, analizado mediante CFD y validado experimentalmente, se utiliza para reproducir el perfil de velocidades típico presente en torno al intercambiador en una circunstancia real de operación. Tras definir métricas relevantes que permitan analizar el comportamiento de las distintas geometrías, se llega a la conclusión de que los problemas aerodinámico y térmico están altamente acoplados en estos dispositivos, demostrando la necesidad de un cuidadoso diseño para mejorar las actuaciones del intercambiador. Los resultados muestran que puede llegar a obtenerse una mejora de más de un 12% en la caída de presión y casi un 20% en el intercambio de calor. Además, se ha confirmado el impacto del uso de la pantalla de distorsión, con variaciones del orden de 10% en ambas variables. Los resultados también muestran que es posible realizar una caracterización preliminar de manera fiable con un modelo impreso en 3D, en cuanto campos de velocidades, pérdidas de presión y frecuencias propias corregidas. Con el análisis llevado a cabo en esta tesis, se puede concluir que es fundamental tener una instalación experimental que reproduzca las condiciones de funcionamiento reales de un motor para realizar estudios relevantes de intercambiadores de calor. Además, es necesario el uso de métricas adecuadas junto con el desarrollo de una metodología exhaustiva, fiable y robusta. Los resultados y metodología presentados en en esta investigación pueden llegar a tener un impacto importante tanto a nivel académico como industrial, ya que abren la puerta a desarrollar sistemas de gestión térmica más eficiente en unas etapas de diseño preliminares que son más asequibles económicamente, consumen menos tiempo y tienen mayor flexibilidad para introducir modificaciones. / [CAT] En un present marcat per la lluita contínua contra la contaminació i el canvi climàtic, la recerca en millores tecnològiques que permetin una transició acceptable per a la societat cap a un futur més ecològic ocupa un paper fonamental. En concret, l'aviació és un focus constant d'innovació, ja que és considerada una funció indispensable en una societat tan globalitzada com l'actual però amb uns nivells de contaminació preocupants. En aquest aspecte, el desenvolupament de motors amb altes eficiències és un pas clau per a la transició mediambiental. Tot i això, aquestes alternatives presenten un repte tecnològic quant a la seva gestió tèrmica basat, principalment, en la necessitat d'augmentar la refrigeració. En aquest context i impulsada pel projecte "Aerodynamic upgrade of Surface Air Cooled Oil Cooler (SACOC)" de Clean Sky 2, aquesta tesi doctoral se centra en l'estudi experimental de geometries d'intercanviadors de calor de superfície per a la refrigeració de l'oli motor mitjançant l'ús de l'aire del flux secundari del turbofan. Actualment, hi ha una sèrie de limitacions quant a les capacitats per dur a terme una anàlisi i disseny detallat d'aquest tipus d'intercanviadors de calor a causa de la manca d'instal·lacions que permetin una caracterització experimental correcta, completa i robusta. Les fonts de dades principals es basen en càlculs numèrics validats a partir d'extrapolacions en condicions de qüestionable aplicabilitat. Al llarg de la tesi es presenten els resultats obtinguts després d'una detallada caracterització de quatre geometries diferents d'intercanviadors de calor emprant tant tècniques intrusives com òptiques. Sutilitza un banc de flux capaç de generar un corrent daire típic al voltant dels intercanviadors, mentre que un sistema de condicionament doli controla el punt doperació pel costat calent. Per recrear de manera més realista les condicions de funcionament, es presenta una metodologia per generar de manera automàtica pantalles de distorsió que poden reproduir una distribució bidimensional de velocitats objectiu mitjançant la manufactura additiva de panells de porositat variable. Aquest model, analitzat mitjançant CFD i validat experimentalment, sutilitza per reproduir el perfil de velocitats típic present al voltant de lintercanviador en una circumstància real doperació. Després de definir mètriques rellevants que permetin analitzar el comportament de les diferents geometries, s'arriba a la conclusió que els problemes aerodinàmic i tèrmic estan altament acoblats en aquests dispositius, demostrant la necessitat d'un disseny acurat per millorar les actuacions de l'intercanviador. Els resultats mostren que es pot arribar a obtenir una millora de més d'un 12% a la caiguda de pressió i gairebé un 20% a l'intercanvi de calor. A més, s'ha confirmat l'impacte de l'ús de la pantalla de distorsió, amb variacions de l'ordre del 10% a les dues variables. Els resultats també mostren que és possible fer una caracterització preliminar de manera fiable amb un model imprès en 3D, en tant que camps de velocitats, pèrdues de pressió i freqüències pròpies corregides. Amb l'anàlisi duta a terme en aquesta tesi, es pot concloure que és fonamental tenir una instal·lació experimental que reprodueixi les condicions de funcionament reals d'un motor per fer estudis rellevants d'intercanviadors de calor. A més, cal fer servir mètriques adequades juntament amb el desenvolupament d'una metodologia exhaustiva, fiable i robusta. Els resultats i metodologia presentats en aquesta investigació poden arribar a tenir un impacte important tant a nivell acadèmic com industrial, ja que obren la porta a desenvolupar sistemes de gestió tèrmica més eficient en unes etapes de disseny preliminars que són més assequibles econòmicament, consumeixen menys temps i tenen més flexibilitat per introduir modificacions. / [EN] In a present marked by the continuous fight against pollution and climate change, research into technological improvements that allow an acceptable transition for society towards a greener future occupies a fundamental role. Specifically, aviation is a constant focus of innovation, since it is considered an essential function in a society as globalized as today's, but with worrying levels of pollution. In this regard, the development of motors with high efficiencies is a key step for the environmental transition. However, these alternatives present a technological challenge in terms of their thermal management, based mainly on the need to increase cooling. In this context and promoted by the Clean Sky 2 "Aerodynamic upgrade of Surface Air Cooled Oil Cooler (SACOC)" project, this doctoral thesis focuses on the experimental study of surface heat exchanger geometries for engine oil cooling using the use of secondary flow air from the turbofan. Currently, there are a number of limitations regarding the capacity to carry out a detailed analysis and design of this type of heat exchanger due to the lack of facilities that allow a correct, complete and robust experimental characterization. The main data sources are based on numerical calculations validated from extrapolations under conditions of questionable applicability. The thesis presents results after a detailed characterization of four different geometries of heat exchangers using both intrusive and optical techniques. A flow bench capable of generating a typical air current around the exchangers is used, while an oil conditioning system controls the point of operation on the hot side. To more realistically recreate operating conditions, a methodology is presented to automatically generate distortion screens that can reproduce a two-dimensional distribution of target velocities through additive manufacturing of variable porosity panels. This model, analyzed by means of CFD and validated experimentally, is used to reproduce the typical speed profile present around the exchanger in a real operating circumstance. After defining relevant metrics that allow analyzing the behaviour of the different geometries, it is concluded that aerodynamic and thermal problems are highly coupled in these devices, demonstrating the need for careful design to improve the exchanger's performance. The results show that an improvement of more than 12% in pressure drop and almost 20% in heat exchange can be obtained. In addition, the impact of using the distortion screen has been confirmed, with variations of the order of 10% in both variables. The results also show that it is possible to carry out a preliminary characterization in a reliable way with a 3D printed model, in terms of velocity fields, pressure losses and corrected eigenfrequencies. With the analysis carried out in this thesis, it can be concluded that it is essential to have an experimental installation that reproduces the real operating conditions of an engine to carry out relevant studies of heat exchangers. In addition, the use of appropriate metrics is necessary together with the development of a comprehensive, reliable and robust methodology. The results and methodology presented in this research can have a significant impact both at an academic and industrial level, since they open the door to developing more efficient thermal management systems in preliminary design stages that are more affordable, consume less time and have more flexibility to make changes. / The respondent wishes to acknowledge the financial support received through the Programa de Apoyo para la Investigación y Desarrollo (PAID) of Univer- sitat Politècnica de València under grant PAID-01-20 n◦ 21589. This project has received funding from the Clean Sky 2 Joint Undertak- ing under the European Unions Horizon 2020 research and innovation pro- gramme under grant agreement No 831977 Aerodynamic upgrade of Sur- face Air-Cooled Oil Coolers (SACOC) / Felgueroso Rodríguez, A. (2023). Aerothermal Characterisation of Surface Heat Exchangers for Turbofans [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/195852