Human α-N-acetylglucosaminidase (Naglu) is a lysosomal enzyme implicated in the rare metabolic storage disorder Mucopolysaccharidosis III type B (MPS IIIB). A deficiency in Naglu results in a buildup of heparan sulfate in lysosomes, which is most detrimental in the central nervous system, causing mental retardation and a shortened lifespan. Enzyme replacement therapy is currently ineffective in treating the neurological symptoms of MPS IIIB due to the inability of Naglu to cross the blood-brain barrier. This laboratory uses a Spodoptera frugiperda insect cell system to express recombinant Naglu conjugated to a synthetic protein transduction domain with the intent to allow Naglu to cross the blood-brain barrier and treat the neurological symptoms. In the present study, we aimed to purify a recombinant Naglu-PTD4 fusion protein in order to assess its capacity to cross cellular membranes. A three-step method involving multi-modal, hydrophobic interaction, and gel filtration chromatography was optimized to achieve pure Naglu-PTD4, in good yield. Cellular uptake by human MPSIIIB fibroblasts of Naglu-PTD4 was not detectable. It is hypothesized that additional amino acids, including a hexahistidine domain, following the PTD4 domain limited the fusion protein’s membrane transduction capacity. Future studies will focus on removing the additional amino acids and adjusting the purification method as necessary. The ultimate goal of this research is to develop a large-scale recombinant Naglu production protocol for enzyme replacement therapy of MPS IIIB. / Graduate

Scholars have been conflicted whether descriptive representation of women leads to substantive representation. A new way of measuring this relationship is through the relationship between women movements and female parliamentarians. Thus, this paper develops from the rethinking of the critical mass theory and uses the feminist demands stated by women organisations in Uganda. This in order to establish whether or not there exists an alliance between inside and outside actors as a measure of substantive representation of women. By applying this approach to transcripts from plenary debates in the Ugandan parliament, the study finds that several gender-related issues are addressed by female MPs. Using a frame analysis comparing the framing of problems between the women organisations and the female MPs, the paper discovers that the majority of issues addressed in the parliament is framed less radically by the female MPs. While the organisations frame the problems as being gender-related, mainly affecting rural women and girls, the MPs frame them more of concern for the whole population and as problems with economic implications for the country.

Perfectionism has long been recognized as a factor that is central to understanding psychological disorders, as it is significantly higher in the majority of psychological disorders compared to the general population. The construct of perfectionism was examined in this research by exploring differences between positive and negative perfectionism. The literature to date has focused almost exclusively on perfectionism as a maladaptive construct, with little research examining if perfectionism can be a positive factor. The implication of the study was to determine if some factors identified may be potentially important in future treatments targeted towards perfectionism, as there is some evidence to suggest that perfectionism may predict poorer response to standard cognitive behavioural treatment. This research compared three different groups; (i) a clinical group with diagnoses of anxiety and depression (n = 40); (ii) a group of athletes (n = 111) and (iii) a student control group (n = 101). The research consisted of 5 studies. In Study 1, evidence was found for the validity, consistency of factor structure and internal consistency of the Positive and Negative Perfectionism Subscale (PANPS; Terry-Short, Owens, Slade, & Dewey, 1995). In Study 2, clinical participants with a range of diagnoses were found to have significantly higher overall perfectionism and negative perfectionism compared to athletes and controls. Rigidity predicted higher positive perfectionism. Dichotomous thinking accounted for a large proportion of variance in negative perfectionism, and was argued to be an important factor distinguishing between positive and negative perfectionism. In Study 3, positive perfectionism was found to relate to faster performance time in athletes competing in triathlons, and negative perfectionism was not found to impede sporting performance. / The Big Five personality domains were investigated in Study 4, and Agreeableness was found to be a significant predictor of negative perfectionism in the clinical group. In comparing clinical and athlete groups, athletes had significantly lower Neuroticism, and higher Extraversion and Conscientiousness. Study 5 was a clinical descriptive study that examined motivation to change and cognitions about failure in a select sample of clinical participants with extreme high scores on negative perfectionism and athletes with extreme low scores. The clinical participants reported many negative consequences, yet despite this recognition, the majority reported they did not wish to change perfectionism. Also, as the level of negative perfectionism increased, the degree of diagnostic comorbidity increased. It was concluded that it may be more useful to distinguish between positive achievement striving and negative perfectionism rather than positive and negative perfectionism. Clinical implications were outlined which included targeting dichotomous thinking and resistance to change in the development of treatments for perfectionism.

This report is a result of a project that been conducted on System Andersson. System Andersson sells and develops MPS-systems for small and medium sized manufacturing companies. System Andersson are today located in the central parts of Jönköping and has about 20 employees. The goal for the project is to develop a new version of Andersson Qwick mps. The project has been divided into a number of smaller projects were the authors been part of the planning module project. The purpose of this part of the project is to investigate which functions that is going to be included in the new MPS-system. The authors part of the projects started off from formulations of questions that been stated in the initial phase of the project.   Two perspectives is brought forward in this report were the authors present what functions  that is available in system today and also which functions that System Anderssons future customers have the need for. The methods used are mapping, interviews and also literature studies that been of great use to answer the questions stated. A mapping of five MPS-systems, including System Andersson, Jeeves, Pyramid, EQ-Plan and Monitor has been conducted.   The results from the review of the mapping shows that System Andersson has the least amount of functions, 21 out of 51. Jeeves offered the greatest amount of functions and thereafter Monitor, Pyramid and EQ-Plan. The area where System Andersson performed best was in the material planning section. Beyond this mapping a prioritisation of the functions has been made by employees of System Andersson. This prioritization showed that many of the 51 functions received a high priority and a small amount of functions received a low priority. If the mapping and the prioritisation is brought together the results show that there was a great amount of functions that received a  high priority that were not included in the system used and sold by System Andersson today. The second part of the results is a number of interviews were the purpose is to create a reference base were parallels can be drawn between the mapping and the prioritisation made by System Andersson. The study show where System Andersson today is positioned in the volym/variant matrix and were the competitors is situated. The results shows that System Andersson miss a number of functions compared to their target group to supply other manufacturing methods and demands of adjacent markets.   The functions that are desirable to implement in the new system to gain more market shares in adjacent markets is:   <ul type="disc">A Graphical planning tool A net requirement rapport Daily planning tool

Inriktningen på detta examensarbete är kapacitetsbeläggning och uppdragsgivaren är System Andersson som utvecklar, tillverkar och installerar verkstadssystem. Detta arbete är ett led i skapandet av förslag på verkstadssystem som är under utveckling och bakgrunden är att kraven från marknaden har ökat. Syftet med arbetet har varit att ge förslag på hur beläggningsdelen i ett alternativt system kan se ut. Som utgångspunkt har ett antal frågeställningar använts. Exempelvis vad som händer om kapaciteten eller planeringen inte håller samt hur kunskapen om planerarens vardag kan användas. För att genomföra arbetet har författarna använt sig av litteraturstudie som grundar sig på tidigare kurslitteratur på programmet, intervjuer av personal på System Andersson samt egna idéer. System Anderssons kunder är främst små och medelstora svenska tillverkande företag. Det vanligaste är att de arbetar efter principen funktionell verkstad och tillverkning mot order. Inom detta segment, som System Andersson vill fortsätta verka inom, är det viktigt att verkstadssystemet kan ändra operationsföljden och optimera kapacitetsutnyttjandet hos maskinparken och personalen. För att produkten ska bli konkurrenskraftigt måste den även vara anpassad efter de personer som använder sig av det. En del av resultatet består av två versioner av beläggningsmodulen, Det som är nytt i detta förslag mot tidigare såld version är: fler funktioner, dagsplanering, kapacitetstak och grafiskt planeringsverktyg. Författarna har kommit med idéer i programmet om hur detta skall vara lösbart. Programmet ska ur planerarens synvinkel hjälpa till med det dagliga arbete och de problemen som skall lösas innan de uppkommer eller vara smidigare att hantera. Ur System Anderssons synvinkel skall detta kunna användas i marknadsförings- och säljarsyfte när de samtalar med kunder. Light och Advanced. Den förstnämnda är inriktad mot de små företagen och innehåller därför endast de viktigaste funktionerna för beläggningsplanering, i övrigt är den så avskalad som möjligt. Den sistnämnda versionen, Advanced, är anpassad för de något större företagen och kan därför utföra ett större antal uppgifter. Det ska vara lämpat för en heltidsanställd planerare med allt som det innebär. Som komplement till dessa två versioner har författarna utformat en stämpelklocka och en körplan för att skapa en bättre helhetsbild av beläggningsplaneringen. Stämpelklockan används för att samla in information om tillgänglig personalkapacitet och körplanen för att kommunicera med produktionen och uppdatera den tillgängliga maskinkapaciteten. Författarnas fokus har varit att rita upp de övergripande skisserna och inte programmeringen som ligger bakom. / This thesis has been carried out in cooperation with System Andersson that develops, manufactures and installs engineering system. This is a proposal on how a system can be designed because the market has become more demanding. This thesis subject is to come with ideas on how a system might look like. As a starting point, a number of questions were made. For example, what happens if the capacity or planning does not agree with the plane, and how does a planner’s normal day look like. To carry out the work the authors have used the literature review based on previous literature in their program, interviews of personnel at System Andersson and own ideas. System Anderssons customers are mainly small and medium-sized, Swedish manufacturing companies. The most common is that the customers work in a job shop and manufacturing that order. In this segment, that System Andersson will continue to work in, it is important to the engineering system can change the operation sequence and optimize the capacity utilization of machinery and personnel. For the product to be competitive, it must also be designed for the people who use the program. Some of the results consist of two versions of the coating module, What is new in this proposal to the version they are selling today are: more features, daily planning, capacity planning and graphic. The authors have come up with ideas in the program on how this should be soluble. The program is from the scheduler point of view to help with the daily work and solve the problems before they arise or be more flexible to manage. From System Anderssons point of view, this could be used in marketing and promoting their programming when they talk with customers. Light, and Advanced. The first is targeted to small businesses and therefore contains only the most important functions for the coating design; the remainder is as scaled as possible. The latter version, Advanced, is adapted to the somewhat larger firms and can therefore perform a wider range of tasks. It should be suitable for a full-time planner with all that that implies. In addition to these two versions the authors have designed a time clock and a dispatch list to create a better overall picture of occupancy planning. The time clock is used to collect information on available staff capacity and dispatch list to communicate to the manufacturing and update the available machine capacity. The authors' focus has been to draw up the overall sketches and not the program behind.

<p>I dagens hårda konkurrenssituation krävs det mer och mer av företags informations-system. Detta gör att fler och fler företag väljer att implementera ett integrerat affärs-system. Integreringen gör att företag får en enhetlig systemmiljö för hela företagets informationsbehandling. Implementeringen är dock en kostsam och tidskrävande process (Ekstrand, 1998). Mitt arbete behandlar frågeställningarna om varför företag väljer att implementera ett affärssystem, hur implementeringen förlöpte samt hur affärssystemet fungerar när implementeringen är färdig.</p><p>Bland de företag jag vände mig till så var den främsta anledningen till att företag installerar ett affärssystem att systemen är omfattande och integrerade.</p><p>Jag kom även till slutsatsen att det nästan är omöjligt att genomföra en implementering av ett affärssystem helt smärtfritt. Problemen beror dock inte alltid på affärssystemet utan på t.ex. dålig beredskap från företagens sida.</p><p>Sammanfattningsvis fick jag intrycket att de flesta företag är nöjda med sitt val av system och de som inte ännu såg nyttan med systemen trodde att de inom kort skulle göra det.</p>

<p>This thesis describes the development of a SOA-based architecture for integrating large EDI-using manufacturing companies purchasing processes with smaller non-EDI capable manufacturing companies, using online services.</p><p>The underlying need for this project lies with the fact that these small manufacturing companies risk to miss out on business, due to their inability to communicate via the industry standard EDI format. At the same time, getting EDI-capable involves significant investments in software licenses, connectivity services and consulting or training that these small companies may not be ready to make. The mentor company of this thesis project, System Andersson, produces resource planning software for this type of companies and would like to be able to provide them with an easy to use way of “jacking in” EDI support into their businesses, without having to make such significant investments. Ideally this feature would be developed as a standalone subscription based service that could be provided to users in such a way that their existing System Andersson software could connect to it and no further hard- or software would be needed on site. This EDI-enabling should be as easy as to be entirely transparent for the end-user companies. The task handed to the author was thus to develop an architecture for how such a subscription based service could be developed. Furthermore, in order to promote re-use and simplify development, the architecture was to be based on SOA concepts.</p><p>As a result of the project, such an architecture has been developed. The architecture details two services for translating and storing for later delivery a number of EDI message types of the EDIFACT variety. The architecture also specifies communications protocols (SOAP over HTTPS and AS2 over HTTPS) and APIs (web services) for how to communicate with these services. These specifications can be used to implement a system that performs the necessary integration, so that the smaller companies may indeed communicate via EDI.</p><p>The fitness of the developed architecture has been tested by implementing a prototype version of such a system based on it. It has also been validated by way of comparing to how well it adheres to SOA design principles. All in all, this design appears to be quite sound and presents a working solution to the studied problem.</p>

<p>This report is a result of a project that been conducted on System Andersson. System Andersson sells and develops MPS-systems for small and medium sized manufacturing companies. System Andersson are today located in the central parts of Jönköping and has about 20 employees. The goal for the project is to develop a new version of Andersson Qwick mps. The project has been divided into a number of smaller projects were the authors been part of the planning module project. The purpose of this part of the project is to investigate which functions that is going to be included in the new MPS-system. The authors part of the projects started off from formulations of questions that been stated in the initial phase of the project.</p><p> </p><p>Two perspectives is brought forward in this report were the authors present what functions  that is available in system today and also which functions that System Anderssons future customers have the need for. The methods used are mapping, interviews and also literature studies that been of great use to answer the questions stated. A mapping of five MPS-systems, including System Andersson, Jeeves, Pyramid, EQ-Plan and Monitor has been conducted.</p><p> </p><p>The results from the review of the mapping shows that System Andersson has the least amount of functions, 21 out of 51. Jeeves offered the greatest amount of functions and thereafter Monitor, Pyramid and EQ-Plan. The area where System Andersson performed best was in the material planning section. Beyond this mapping a prioritisation of the functions has been made by employees of System Andersson. This prioritization showed that many of the 51 functions received a high priority and a small amount of functions received a low priority. If the mapping and the prioritisation is brought together the results show that there was a great amount of functions that received a  high priority that were not included in the system used and sold by System Andersson today. The second part of the results is a number of interviews were the purpose is to create a reference base were parallels can be drawn between the mapping and the prioritisation made by System Andersson. The study show where System Andersson today is positioned in the volym/variant matrix and were the competitors is situated. The results shows that System Andersson miss a number of functions compared to their target group to supply other manufacturing methods and demands of adjacent markets.</p><p> </p><p>The functions that are desirable to implement in the new system to gain more market shares in adjacent markets is:</p><p> </p><ul type="disc"><li>A Graphical planning tool</li><li>A net requirement rapport</li><li>Daily planning tool</li></ul>

Made available in DSpace on 2014-06-12T15:57:17Z (GMT). No. of bitstreams: 2 arquivo3178_1.pdf: 2284280 bytes, checksum: f355230864026f0ced9535d89e5b2f39 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2008 / A Melhoria de Processos de Software (MPS) tem se mostrado, na prática, uma abordagem viável e eficiente para a melhoria das organizações. Contudo, a adoção da MPS envolve inúmeros desafios e dificuldades, os quais contribuem para que várias iniciativas de adoção da MPS acabem fracassando. A MPS baseada em modelos da qualidade (model based software process improvement) com níveis fixos de maturidade, não atendem a diversidade de empresas da indústria de software. A MDPEK têm se apresentado como uma forma alternativa e bastante promissora para a adoção de MPS. Na melhoria dirigida a Perfil de Capacidade de Processo (PCP) os processos a serem melhorados não estão prefixados, estes devem ser definidos de acordo com os objetivos, estratégias e contextos de negócio das organizações. No contexto das empresas dirigidas a evolução de produtos de software, é bastante nítida a dificuldade em implantação dos modelos da qualidade baseados em níveis pré-fixados de maturidade. Muito embora, os benefícios obtidos com adoção destes modelos são reconhecidos. Este tipo de empresa apresenta características particulares que dificultam a adoção da MPS de forma tradicional. Esta dissertação aplica os conceitos da MDPEK para a definição de um PCP, baseado no CMMI, e direcionado ao domínio específico das empresas dirigidas à evolução de produto de software. A abordagem PRO2PI é utilizada como referência para guiar a execução das atividades de definição do PCP. O qual é proposto tomando como base as observações e estudos realizados em um grupo de oito empresas de dirigidas à evolução de produto de software, e posteriormente validado em duas empresas que aplicaram a MPS e foram, recentemente, avaliadas no nível 2 de maturidade do CMMI

This thesis describes the development of a SOA-based architecture for integrating large EDI-using manufacturing companies purchasing processes with smaller non-EDI capable manufacturing companies, using online services. The underlying need for this project lies with the fact that these small manufacturing companies risk to miss out on business, due to their inability to communicate via the industry standard EDI format. At the same time, getting EDI-capable involves significant investments in software licenses, connectivity services and consulting or training that these small companies may not be ready to make. The mentor company of this thesis project, System Andersson, produces resource planning software for this type of companies and would like to be able to provide them with an easy to use way of “jacking in” EDI support into their businesses, without having to make such significant investments. Ideally this feature would be developed as a standalone subscription based service that could be provided to users in such a way that their existing System Andersson software could connect to it and no further hard- or software would be needed on site. This EDI-enabling should be as easy as to be entirely transparent for the end-user companies. The task handed to the author was thus to develop an architecture for how such a subscription based service could be developed. Furthermore, in order to promote re-use and simplify development, the architecture was to be based on SOA concepts. As a result of the project, such an architecture has been developed. The architecture details two services for translating and storing for later delivery a number of EDI message types of the EDIFACT variety. The architecture also specifies communications protocols (SOAP over HTTPS and AS2 over HTTPS) and APIs (web services) for how to communicate with these services. These specifications can be used to implement a system that performs the necessary integration, so that the smaller companies may indeed communicate via EDI. The fitness of the developed architecture has been tested by implementing a prototype version of such a system based on it. It has also been validated by way of comparing to how well it adheres to SOA design principles. All in all, this design appears to be quite sound and presents a working solution to the studied problem.