Mucopolissacaridoses (MPS) são erros inatos do metabolismo, fazem parte das doenças lisossômicas de depósito e ocorrem devido à deficiência na atividade de enzimas que catalisam a degradação de glicosaminoglicanos (GAGs). São doenças crônicas e progressivas causadas pelo acúmulo de um ou mais GAGs nos lisossomos das células. A MPS do tipo IV (síndrome de Morquio) ocorre pelo acúmulo de queratan sulfato e condoitin-6-sulfato, causado pela deficiência de duas diferentes enzimas, que distinguem essa MPS em dois subtipos, deficiência de N-acetilgalactosamina-6-sulfatase (MPS IV tipo A) ou de β-galactosidase (MPS IV tipo B). Na MPS IV A as características clínicas predominantes são relacionadas ao sistema esquelético e na maioria das vezes o paciente tem inteligência normal; não há uma terapia efetiva para a doença, deste modo o diagnóstico precoce direciona para o início de tratamentos paliativos. A investigação inicial dessa MPS se baseia em avaliação clínica, radiografias, quantificação e identificação de GAGs urinários e o diagnóstico definitivo pela medida da atividade enzimática da N-acetilgalactosamina-6-sulfatase (GALNS) em leucócitos ou fibroblastos. O objetivo desse trabalho foi estudar alguns parâmetros bioquímicos da enzima GALNS em leucócitos de indivíduos normais e adaptar a técnica de medida dessa atividade enzimática para sangue impregnado em papel filtro. O Km e a Vmáx da GALNS em leucócitos de indivíduos normais foi de 7,16mM e 77,3nmol/17h/mg de proteína, respectivamente. Também adaptamos a técnica da medida da atividade enzimática dessa enzima utilizando menor concentração de substrato (3mM), esta concentração teve correlação positiva com a concentração 10 mM, que é empregada na técnica original. A partir disto, foram estabelecidos os valores de referência em leucócitos para indivíduos normais e indivíduos afetados com MPS IV A. Os mesmos foram, respectivamente: 11,0 a 101,4 e 0,1 a 0,2 nmol/17h/mg de proteína. Foi também observado que, com o passar do tempo, a temperatura de 70ºC causa uma inativação gradual da atividade enzimática. Por último, apesar de várias alterações na técnica original em leucócitos, não foi possível adaptá-la para ser utilizada na medida da atividade da GALNS em sangue impregnado em papel filtro. A determinação dos parâmetros bioquímicos da GALNS é conveniente devido ao avanço nos estudos clínicos de desenvolvimento da terapia de reposição enzimática aos indivíduos afetados com MPS IV A e na distinção de indivíduos saudáveis, afetados e heterozigotos. Desenvolver técnicas fluorimétricas em amostras de sangue impregnado em papel filtro é de grande importância para serem utilizadas em triagem de recém-nascidos e populações de alto risco para MPS. Devendo ser confirmado por testes de atividade da enzima em leucócitos ou fibroblastos cultivados. A partir dos nossos protocolos, não foi possível desenvolver e padronizar uma técnica para medida da atividade da enzima N-acetilgalactosamina-6-sulfatase em sangue impregnado em papel filtro de modo a triarmos indivíduos com suspeita de MPS IV A. / Mucopolysaccharidosis (MPS) are inborn errors of metabolism, are part of the lysosomal storage diseases and occur due to the deficiency in the activity of enzymes that catalyze the degradation of glycosaminoglycans (GAGs). They are chronic and progressive diseases caused by the accumulation of one or more GAGs in the lysosomes of cells. The MPS type IV (Morquio syndrome) occurs by the accumulation of keratan sulfate and condoitin-6-sulfate, caused by two different enzyme deficiencies, which distinguish this MPS into two subtypes, deficiency of N-acetylgalactosamine-6-sulfatase (MPS IV type A) or the enzyme β-galactosidase (MPS type IV B). In MPS IVA the predominant clinical features are related to the skeletal system and in most cases the patient has normal intelligence. There is no effective therapy for the disease, so early diagnosis directs for the beginning of palliative treatments. The initial investigation of this MPS is based on clinical evaluation, radiographs, quantification and identification of urinary GAGs and the definitive diagnosis by measure of N-acetylgalactosamine-6-sulfatase (GALNS) activity in leukocytes or fibroblasts. The aim of this work was to study some biochemical parameters of the GALNS in leukocytes of normal individuals and adapt the technique of measuring this enzyme activity to blood impregnated on filter paper. The Km and Vmax of GALNS in leukocytes from normal subjects was 7.16 mM and 77.3 nmol/17h/mg of protein, respectively. We also adapted the technique of measuring the enzymatic activity of this enzyme using lower substrate concentration (3mM). This concentration had a positive correlation with 10 mM concentration, which is used in the original technique. From this, the reference values for normal and individuals affected with MPS IVA in leukocytes were established. They were 11.0 to 101.4 and 0.1 to 0.2 of nmol/17h/mg of protein, respectively. It was also observed that over time the temperature of 70°C causes a gradual inactivation of enzyme activity. Finally, despite several changes to the original technique in leukocytes, it has not been possible to adapt it to be used in measuring the activity of GALNS blood impregnated on filter paper. The determination of biochemical parameters of GALNS is convenient due to the advancement in clinical studies for the development of enzyme replacement therapy for individuals affected with MPS IVA and to distinguish between healthy and affected heterozygous individuals. The development of fluorometric techniques in blood impregnated on filter paper is of great importance for use in newborn screening and in high risk populations to MPS. It should be confirmed by tests of enzyme activity in leukocytes or cultured fibroblasts. Unfortunately, from our protocols, it was not possible to develop and standardize a technique for measuring the activity of the enzyme N- acetylgalactosamine-6-sulfatase in blood impregnated on filter paper for to screen individuals suspected of MPS IVA.

Mucopolissacaridoses (MPS) são erros inatos do metabolismo, fazem parte das doenças lisossômicas de depósito e ocorrem devido à deficiência na atividade de enzimas que catalisam a degradação de glicosaminoglicanos (GAGs). São doenças crônicas e progressivas causadas pelo acúmulo de um ou mais GAGs nos lisossomos das células. A MPS do tipo IV (síndrome de Morquio) ocorre pelo acúmulo de queratan sulfato e condoitin-6-sulfato, causado pela deficiência de duas diferentes enzimas, que distinguem essa MPS em dois subtipos, deficiência de N-acetilgalactosamina-6-sulfatase (MPS IV tipo A) ou de β-galactosidase (MPS IV tipo B). Na MPS IV A as características clínicas predominantes são relacionadas ao sistema esquelético e na maioria das vezes o paciente tem inteligência normal; não há uma terapia efetiva para a doença, deste modo o diagnóstico precoce direciona para o início de tratamentos paliativos. A investigação inicial dessa MPS se baseia em avaliação clínica, radiografias, quantificação e identificação de GAGs urinários e o diagnóstico definitivo pela medida da atividade enzimática da N-acetilgalactosamina-6-sulfatase (GALNS) em leucócitos ou fibroblastos. O objetivo desse trabalho foi estudar alguns parâmetros bioquímicos da enzima GALNS em leucócitos de indivíduos normais e adaptar a técnica de medida dessa atividade enzimática para sangue impregnado em papel filtro. O Km e a Vmáx da GALNS em leucócitos de indivíduos normais foi de 7,16mM e 77,3nmol/17h/mg de proteína, respectivamente. Também adaptamos a técnica da medida da atividade enzimática dessa enzima utilizando menor concentração de substrato (3mM), esta concentração teve correlação positiva com a concentração 10 mM, que é empregada na técnica original. A partir disto, foram estabelecidos os valores de referência em leucócitos para indivíduos normais e indivíduos afetados com MPS IV A. Os mesmos foram, respectivamente: 11,0 a 101,4 e 0,1 a 0,2 nmol/17h/mg de proteína. Foi também observado que, com o passar do tempo, a temperatura de 70ºC causa uma inativação gradual da atividade enzimática. Por último, apesar de várias alterações na técnica original em leucócitos, não foi possível adaptá-la para ser utilizada na medida da atividade da GALNS em sangue impregnado em papel filtro. A determinação dos parâmetros bioquímicos da GALNS é conveniente devido ao avanço nos estudos clínicos de desenvolvimento da terapia de reposição enzimática aos indivíduos afetados com MPS IV A e na distinção de indivíduos saudáveis, afetados e heterozigotos. Desenvolver técnicas fluorimétricas em amostras de sangue impregnado em papel filtro é de grande importância para serem utilizadas em triagem de recém-nascidos e populações de alto risco para MPS. Devendo ser confirmado por testes de atividade da enzima em leucócitos ou fibroblastos cultivados. A partir dos nossos protocolos, não foi possível desenvolver e padronizar uma técnica para medida da atividade da enzima N-acetilgalactosamina-6-sulfatase em sangue impregnado em papel filtro de modo a triarmos indivíduos com suspeita de MPS IV A. / Mucopolysaccharidosis (MPS) are inborn errors of metabolism, are part of the lysosomal storage diseases and occur due to the deficiency in the activity of enzymes that catalyze the degradation of glycosaminoglycans (GAGs). They are chronic and progressive diseases caused by the accumulation of one or more GAGs in the lysosomes of cells. The MPS type IV (Morquio syndrome) occurs by the accumulation of keratan sulfate and condoitin-6-sulfate, caused by two different enzyme deficiencies, which distinguish this MPS into two subtypes, deficiency of N-acetylgalactosamine-6-sulfatase (MPS IV type A) or the enzyme β-galactosidase (MPS type IV B). In MPS IVA the predominant clinical features are related to the skeletal system and in most cases the patient has normal intelligence. There is no effective therapy for the disease, so early diagnosis directs for the beginning of palliative treatments. The initial investigation of this MPS is based on clinical evaluation, radiographs, quantification and identification of urinary GAGs and the definitive diagnosis by measure of N-acetylgalactosamine-6-sulfatase (GALNS) activity in leukocytes or fibroblasts. The aim of this work was to study some biochemical parameters of the GALNS in leukocytes of normal individuals and adapt the technique of measuring this enzyme activity to blood impregnated on filter paper. The Km and Vmax of GALNS in leukocytes from normal subjects was 7.16 mM and 77.3 nmol/17h/mg of protein, respectively. We also adapted the technique of measuring the enzymatic activity of this enzyme using lower substrate concentration (3mM). This concentration had a positive correlation with 10 mM concentration, which is used in the original technique. From this, the reference values for normal and individuals affected with MPS IVA in leukocytes were established. They were 11.0 to 101.4 and 0.1 to 0.2 of nmol/17h/mg of protein, respectively. It was also observed that over time the temperature of 70°C causes a gradual inactivation of enzyme activity. Finally, despite several changes to the original technique in leukocytes, it has not been possible to adapt it to be used in measuring the activity of GALNS blood impregnated on filter paper. The determination of biochemical parameters of GALNS is convenient due to the advancement in clinical studies for the development of enzyme replacement therapy for individuals affected with MPS IVA and to distinguish between healthy and affected heterozygous individuals. The development of fluorometric techniques in blood impregnated on filter paper is of great importance for use in newborn screening and in high risk populations to MPS. It should be confirmed by tests of enzyme activity in leukocytes or cultured fibroblasts. Unfortunately, from our protocols, it was not possible to develop and standardize a technique for measuring the activity of the enzyme N- acetylgalactosamine-6-sulfatase in blood impregnated on filter paper for to screen individuals suspected of MPS IVA.

京都大学 / 新制・課程博士 / 博士(工学) / 甲第23477号 / 工博第4889号 / 新制||工||1764(附属図書館) / 京都大学大学院工学研究科社会基盤工学専攻 / (主査)教授 後藤 仁志, 教授 戸田 圭一, 准教授 原田 英治 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

This thesis deals with the occurrence of microplastics in water, the reasons for their potential elimination, and the possibilities leading to that. More specifically, it focuses on flotation as a process for removing microplastics from water. Flotation is further described from a theoretical point of view and its mathematical model is developed. The wettability of selected polymers is measured. The experiment performed to determine the efficiency of flotation as a process of treating water from microplastics is described. This efficiency is further compared to measured wettability.

Die Fetale Akinesia Deformation Sequence (FADS) umfasst ein breites klinisches Spektrum. Dieses reicht von Tot- und Fehlgeburten, fetalen Ödemen bis hin zu Kontrakturen, Pterygien und Atemschwäche. Die Ätiologie der FADS ist sehr heterogen. Der Fokus dieser Forschungsarbeit lag in der Mutationsanalyse in Genen des Acetylcholin-Rezeptor-Pathways. Bekannt war das homozygote missense und nonsense Mutationen in den Genen der fetalen Untereinheit CHRNG des Rezeptors mit dem klinischen Bild des multiple Pterygien-Syndrom (MPS) und Letalen multiplen Pterygien-Syndrom (LMPS) einhergehen. Missense Mutationen in weiteren Genen des AChR-Komplexes präsentieren sich klinisch als Congenitales Myasthenes Syndrom (CMS). Vermutet, aber nicht bewiesen war das homozygote nonsense Mutationen in diesen weiteren Genen letal verlaufen und ursächlich für das letale Pterygiensyndrom sein können. Wir konnten diese Hypothese bestätigen.:1. Einführung in das Thema 1.1. Prävalenz und Relevanz fetaler Bewegungsstörungen und angeborener Kontrakturen 1.2. Das klinische Bild 1.3. Ursachen verminderter fetaler Bewegung 1.4. Der nicotinerge Acetylcholinrezeptor 1.5. Mutationssuche in den Genen der α1-, β1- und δ-Untereinheit (CHRNA1, CHRNB1 und CHRND) sowie in dem Rezeptor assoziierten RAPSN-Gen 2. Publikation 3. Zusammenfassung der Arbeit Literaturverzeichnis Anlagen Darstellung des eigenen wissenschaftlichen Beitrags Erklärung über die eigenständige Abfassung der Arbeit

Contrast agents for magnetic resonance imaging (MRI) are but one of a variety of nanosystems that have incredible potential for the detection and diagnosis of cancer. Nanosystems share a common disadvantage: they are quickly sequestered by biological processes that clear foreign material from the body, requiring ever larger doses to accumulate in targets, and reducing their overall effectiveness and viability. This thesis explores a pair of strategies for nanomaterials to boost their evasiveness from these defensive systems in the context of lanthanide MRI contrast agents, in an attempt to increase their probability to collect in cancerous tissue. Chapter 1 provides precedent and rationale for the modification of two parameters regarding novel nanosystem design: aspect ratio and zeta potential. Chapter 2 details the controlled syntheses and analysis of sodium dysprosium fluoride nanomaterials at a range of aspect ratios. Chapter 3 concerns the construction of tunable zwitterionic polymer coatings for synthesized nanomaterials to demonstrate control over the zeta potential in aqueous dispersion. Chapter 4 tests polymer-coated spherical nanoparticles and nanorods for internalization into or adsorbance onto a cancerous cell line. Chapter 5 summarizes the work of the previous chapters and suggests future research approaches. Though internalization or adsorbance onto HeLa cells was not observed for prepared nanomaterials, control over their aspect ratio at the synthetic level and zeta potential via constructed zwitterionic polymers was demonstrated, with implications for application to a plethora of nanosystems. / Graduate

Syftet med den här studien är att undersöka lutning och höjd för vägräcken och hur dessa faktorer påverkar trafiksäkerheten. Rapporten syftar även till att ta upp den säkerhetsproblematik som rör MC-trafikanter då underglidningsskydd inte får plats att monteras under vägräckena. Genom forskning i Trafikverkets och Statens väg- och transportinstituts databaser samt intervjuer tillsammans med föreningarna SMC (Svenska MotorCyklister) och SVBRF (Sveriges bro- och vägräckesförening) har det framkommit överensstämmande resultat gällande den nationella, svenska standarden SS EN-1317. Den standarden vi använder idag, SS EN-1317 ger en monteringshöjd på 450 millimeter till bärande balk. Vilket ger problematik vid montering av underglidningsskydd och påverkar trafiksäkerheten negativt. I nuläget hålls en vetenskaplig forskning gällande underglidningsskydd som testas på fyra olika vägar i Sverige, bland annat i Åkersberga, Stockholm. / The purpose of this study is to survey the incline and height of crash barriers and how these two factors affect traffic security. The study also serves the purpose of bringing up problems in traffic security due to lack of space under crash barriers for installation of slide guards for motorcyclists. Research in the databases of the Swedish Transportation Administration (Trafikverket), Road and Transport Institute (VTI), the Associations of Swedish Motor Cyclists (föreningen Sveriges MotorCyklister), and Swedish Bridge and Crash Barrier Association (Sveriges Bro- och Vägräckesförening) has shown corresponding results with the Swedish standard SS EN-1317. The standard in use today, SS EN-1317, affects traffic security negatively due to the height of assemblage of the carrying beam in crash barriers being 450 millimeters. At the time of writing research is being conducted regarding slide guards on four different roads in Sweden with one located in Åkersberga, Stockholm.

Made available in DSpace on 2016-03-15T19:32:20Z (GMT). No. of bitstreams: 1 Eduardo Frare.pdf: 961050 bytes, checksum: ebc8f1878e5b39a9e1c7c67c56a33eed (MD5) Previous issue date: 2010-08-10 / Fundo Mackenzie de Pesquisa / In the late 1990s, in the context of reform of the Brazilian state was created the model of Social Organization with the aim of expanding the managerial capacity of institutions operating public resources, but whose activities are not exclusive state. In mid 1997, the Brazilian Association of Synchrotron Light Technology - ABTLuS was qualified as an OS to operate the Brazilian Synchrotron Light Laboratory - LNLS, organization object of this Case Study. The adoption of the OS model and the requirements for establishing the parameters for results through the Management Contract signed between the ABTLuS and the Ministry of Science and Technology - MCT, has led to new artifacts management were deployed in the LNLS. Among the major contributions of the OS model is the identification of measures to monitor performance in organizations of Science, Technology and Innovation - C, T & I. The survey was conducted using the qualitative method, exploratory and applied using a procedure to collect data, apply in-depth interviews with a member of the Board, four directors or former directors of LNLS, two group leaders and three agents of the external evaluation organization. The collected data were analyzed using content analysis with triangulation. The research identified key performance measures for monitoring the LNLS which were grouped into two perspectives: i) the strategic and ii) of inducing results. The accompanying measures of the strategy are: i) Legitimacy, ii) Research Results; iii) Reliability of Facilities and iv) Open of Facilities. Accompanying measures known inducers of results was composed by the aggregation of the following categories: i) Human Resources, ii) Financial Resources and iii) Managerial Efficiency. Finally, the thesis presents a conceptual model for monitoring the performance measures for organizations, C, T & I and the practical point of view, suggesting a new framework of performance indicators for the LNLS. / No final da década de 1990, no contexto da Reforma do Estado brasileiro, foi criado o modelo de Organização Social com o objetivo de ampliar a capacidade gerencial de instituições que operam recursos públicos, mas cujas atividades não são exclusivas de Estado. Em meados de 1997, a Associação Brasileira de Tecnologia de Luz Síncrotron ABTLuS foi qualificada como OS para operar o Laboratório Nacional de Luz Síncrotron LNLS, entidade objeto desse estudo de caso. A adoção do modelo de OS e as exigências de estabelecer os parâmetros de resultados por meio do contrato de gestão, firmado entre a ABTLuS e o Ministério da Ciência e Tecnologia - MCT, fez com que novos artefatos gerenciais fossem implantados no LNLS. Dentre as principais contribuições do modelo de OS é a identificação de medidas para o acompanhamento de desempenho em organizações de Ciência, Tecnologia e Inovação C,T&I. A pesquisa foi realizada com o uso do método qualitativo, do tipo exploratório e aplicado, utilizando como procedimento de coleta de dados, entrevistas em profundidade aplicadas com um membro do Conselho de Administração, quatro dirigentes ou ex-dirigentes do LNLS, dois líderes de grupo, e três agentes de avaliação externos à organização. Os dados coletados foram analisados por meio de análise de conteúdo, com triangulação. A pesquisa identificou as principais medidas de desempenho para o acompanhamento do LNLS que foram agrupadas em duas perspectivas: i) a da estratégica propriamente dita e ii) a de indutores de resultados. As medidas de acompanhamento da estratégia foram: i) Legitimidade, ii) Resultados Científicos; iii) Confiabilidade das Instalações; iv) Disponibilidade das Instalações. As medidas de acompanhamento denominadas indutores de resultados foram compostas pela agregação das seguintes categorias: i) Recursos Humanos; ii) Recursos Financeiros; e iii) Eficiência Gerencial. Por fim, a dissertação, apresenta um modelo conceitual para acompanhamento das medidas de desempenho para organizações de C,T&I e do ponto de vista prático sugere-se um novo quadro de indicadores de desempenho para o LNLS.

Quantification of stochastic or quantum systems by a joint probability density or wave function is a notoriously difficult computational problem, since the solution depends on all possible states (or realizations) of the system. Due to this combinatorial flavor, even a system containing as few as ten particles may yield as many as $10^{10}$ discretized states. None of even modern supercomputers are capable to cope with this curse of dimensionality straightforwardly, when the amount of quantum particles, for example, grows up to more or less interesting order of hundreds. A traditional approach for a long time was to avoid models formulated in terms of probabilistic functions, and simulate particular system realizations in a randomized process. Since different times in different communities, data-sparse methods came into play. Generally, they aim to define all data points indirectly, by a map from a low amount of representers, and recast all operations (e.g. linear system solution) from the initial data to the effective parameters. The most advanced techniques can be applied (at least, tried) to any given array, and do not rely explicitly on its origin. The current work contributes further progress to this area in the particular direction: tensor product methods for separation of variables. The separation of variables has a long history, and is based on the following elementary concept: a function of many variables may be expanded as a product of univariate functions. On the discrete level, a function is encoded by an array of its values, or a tensor. Therefore, instead of a huge initial array, the separation of variables allows to work with univariate factors with much less efforts. The dissertation contains a short overview of existing tensor representations: canonical PARAFAC, Hierarchical Tucker, Tensor Train (TT) formats, as well as the artificial tensorisation, resulting in the Quantized Tensor Train (QTT) approximation method. The contribution of the dissertation consists in both theoretical constructions and practical numerical algorithms for high-dimensional models, illustrated on the examples of the Fokker-Planck and the chemical master equations. Both arise from stochastic dynamical processes in multiconfigurational systems, and govern the evolution of the probability function in time. A special focus is put on time propagation schemes and their properties related to tensor product methods. We show that these applications yield large-scale systems of linear equations, and prove analytical separable representations of the involved functions and operators. We propose a new combined tensor format (QTT-Tucker), which descends from the TT format (hence TT algorithms may be generalized smoothly), but provides complexity reduction by an order of magnitude. We develop a robust iterative solution algorithm, constituting most advantageous properties of the classical iterative methods from numerical analysis and alternating density matrix renormalization group (DMRG) techniques from quantum physics. Numerical experiments confirm that the new method is preferable to DMRG algorithms. It is as fast as the simplest alternating schemes, but as reliable and accurate as the Krylov methods in linear algebra.

Eine wichtige Eigenschaft von Leistungsdioden ist ihre Stoßstromrobustheit, also die Fähigkeit, einem kurzeitigen hohen Strom standzuhalten. Bei Dioden aus SiC wird dabei häufig auf die MPS-Struktur zurückgegriffen. In dieser Arbeit wird das Stoßstromverhalten von neuen 1200V-SiC-MPS-Dioden von Infineon untersucht. Dabei wird gemessen, welchem Strom die Dioden bei einem Halbsinus-Puls von 10ms standhalten. Das Ergebnis wird mit der Robustheit herkömmlicher SiC-Schottky-Dioden verglichen. Die Stoßstromrobustheit bei Parallelschaltung wird untersucht. Mittels elektro-thermischer Simulationen werden Möglichkeiten zur Verbesserung der Stoßstromrobustheit der Dioden erörtert. Hierbei wird die Schichtdicke erhöht und Kupfer statt Aluminium als Anodenmetallisierung angenommen. Des Weiteren wird das Simulationsmodell hinsichtlich der Lotschicht und eines reduzierten SiC-Substrats variiert. Die MPS-Dioden weisen bei hohem Strom einen negativen differentiellen Widerstand auf, hervorgerufen durch die Injektion von Minoritätsladungsträgern aus den p-dotierten Gebieten. Die Aktivierung der Injektion von Minoritätsladungsträgern in Abhängigkeit der Größe der p-dotierten Gebiete wird ebenfalls mittels Simulationen untersucht. Ein Vergleich verschiedener Caughey-Thomas-Parameter zur Modellierung der Ladungsträgermobilität in FEM-Bauelementsimulatoren wird durchgeführt. Das Ausschaltverhalten der MPS-Dioden wird unter verschiedenen Bedingungen gemessen. Dazu zählen das Abschalten unter Anwendungsbedingungen, das Abschalten unter Überlast von bis zum fünfzehnfachen Nennstrom, das Abschalten mit hohen Strom- und Spannungssteilheiten und das Abschalten in Parallelschaltung und mit zusätzlicher parasitärer Induktivität. Die Untersuchungen zeigen eine hohe Robustheit der neuen 1200V-SiC-MPS-Dioden. / The surge current ruggedness is an import property of power diodes. In case of SiC diodes this is realized by a Merged-pin-Schottky (MPS) structure. In this thesis the surge current ruggedness of novel 1200V SiC MPS diodes from Infineon is investigated. The maximum current during a half sine surge current pulse of 10ms is determined in measurements for various diodes and compared to the surge current ruggedness of conventional Schottky diodes. Furthermore, the surge current ruggedness in parallel arrangement is measured. By the means of electro-thermal simulations options to improve the surge current ruggedness are investigated. The simulation model is varied concerning the diodes anode metalization layer thickness and material. The layer thickness is increased and the typical aluminum is replaced by copper. Additionally, the influence of the silicon carbide substrate thickness and the solder layer thickness and material on the surge current ruggedness is simulated. The MPS diodes possess a negative differential resistance at high currents caused by the injection of minority carriers by the p-doped regions. The injection of minority carriers in dependence of the size of the p-regions is also examined in simulations. Furthermore, different parameter sets of the the Caughey-Thomas formula to describe the carrier mobility are compared. The turn-off behavior of the diodes is measured under different conditions such as the turn-off from fifteen times the rated current, the turn-off with high current and voltage slopes and the turn-off in parallel arrangement and with additional inductance. The investigation show a high robustness of the novel 1200V SiC MPS diodes.