Diet and movements in fishes are often logistically challenging to study. Trace element and stable isotope analyses have advanced these fields considerably, but are still constrained by methodological impediments, such as the tendency towards lethal sampling. Studying endangered fishes is particularly challenging as representative samples are difficult to obtain. However, the information gained from such studies is often critical to the recovery of endangered fishes as knowledge of life history attributes has the potential to greatly influence the success of management strategies. I tested the viability of using fin rays in fishes as a non-lethal approach to study diet and movement patterns over time. I then applied the methods I developed to study the life history of the critically endangered Goliath Grouper, Epinephelus itajara. Fin ray analyses have traditionally been used in age and growth studies, as well as in a limited number of projects that study the chemical constituents of the ray itself. Therefore, I first tested whether fin rays could be used as chronological recorders of chemical properties over time using a pseudo-experimental design. By using samples from various aquaria, I had documentation of the time of capture of every animal used. Based on the assumption that the otolith in fishes represents a conserved, chronological matrix, I compared trace element concentrations between the otolith and the fin ray of each individual. In addition I tested whether stable isotope values of δ13C and δ15N differed between the wild and captive life phases of each individual. Divalent ions and positively-charged transition metals (e.g., Fe, Co) in particular showed strong associations between the two structures, suggesting conservation of material. Stable isotope values of δ13C and δ15N differed between the wild and captive life phases in most of the fishes sampled, also suggestive of conserved matrices. I then tested and modeled the differences in δ15N values over time between the populations of Goliath Groupers on the west and east coasts of Florida. In general, individuals on the west coast had lower overall values and a larger difference between juvenile and adult values. The mechanism that caused the differences between coastal populations may have been an artifact of the environment, rather than different feeding behaviors. Last, I investigated different nursery habitats for Goliath Groupers in southwestern Florida. I characterized juvenile nurseries based on the chemical fingerprints of trace elements within the inorganic matrix of the fin rays. Groupings based on these fingerprints were surprisingly accurate and can be used to identify essential nursery habitat for the species in years to come. This research demonstrated the efficacy of novel techniques that were used to gather information on the life history of a critically endangered fish in the state of Florida. The results can be used to influence management strategies in the future, particularly with regard to nursery habitat use.

This master´s thesis describes the design and processing of a business plan for the establishment of private nursery school in Jihlava. The proposal responds to a market gab in providing nursery school services.The theoretical part defines the basic concepts related to business in this field and analyzes the structure of the business plan. The practical part is focused on the analysis of the environment and the specific development of a business plan including risk assessment and feasibility evaluation of projects.

När barn tar steget över från förskola till förskoleklass är det för många av dem början till nya kunskaper. De byter fysisk miljö, lärare och behöver också många gånger skapa nya kompisrelationer. Vår intervjustudie med lärare i förskoleklass och förskollärare i förskolan ska bidra med kunskap kring hur de samverkar med varandra vid barns övergång från förskola till förskoleklass för att kunna skapa kontinuitet i undervisningen. Detta är en kvalitativ studie med semistrukturerade intervjuer som underlag där vi intervjuat tre förskollärare och tre lärare i förskoleklass. Vi har använt oss av en kvalitativ metod där det är respondenternas svar som står i centrum. Genom att repetitivt läsa materialet har vi sedan kunnat skapa olika kategorier utifrån respondenternas svar.   Vår studie visar att förskollärare och lärare i förskoleklass tycker det är viktigt att förbereda barnen inför övergång från förskola till förskoleklass och att samverkan är viktigt för att kunna skapa en röd tråd i undervisningen. Vår studie visar också att det finns flera hinder i samverkan mellan förskola och förskoleklass. Dessa hinder kan vara av organisationsmässig karaktär och att lärare i förskoleklass och förskollärare inte har tillräckligt med insyn i varandras verksamheter. Detta leder i sin tur till att all information inte kommer fram och att det blir svårt för lärare i förskoleklass att skapa en kontinuitet i undervisningen.

Les écosystèmes côtiers et estuariens sont extrêmement riches d’un point de vue biologique et écologique et abritent des habitats essentiels au maintien et au renouvellement des ressources marines d’intérêt halieutique. Parmi les altérations subies par ces écosystèmes, les proliférations de macroalgues opportunistes sont l’un des signes les plus visibles de l’eutrophisation dans plusieurs régions du monde, et particulièrement dans le nord-ouest de la France. Les processus à l’origine de ces proliférations sont relativement bien identifiés tandis que leurs conséquences écologiques - notamment sur l’ichtyofaune - restent mal connues et peu étudiées. L’objectif de cette thèse est de caractériser les effets des marées vertes sur la fonction d’habitat halieutique essentiel au renouvellement de l’ichtyofaune des zones côtières et estuariennes par une approche multi-échelleLes conséquences des marées vertes sur l’ichtyofaune ont tout d’abord été examinées à l’échelle de la communauté. Ensuite, les processus par lesquels cette perturbation affecte l’ichtyofaune ont été étudiés par une approche à l’échelle de l’individu. Cette étude s’est intéressée aux réponses des juvéniles à travers la sélection de l’habitat et la comparaison de leurs performances physiologiques entre un site contrôle et un site impacté. Au travers de ces deux approches, un impact négatif écologiquement significatif des marées vertes sur la qualité des habitats essentiels au renouvellement des ressources d’intérêt halieutique a été mis en évidence. Les conséquences des marées vertes sur l’ichtyofaune sont modul / Coastal and estuarine systems are highly productive areas that provide unique biological and ecological services and shelter essential habitats to numerous fisheries resources. Among the perturbations experienced by these ecosystems, proliferations of opportunistic macroalgae, commonly known as ‘green tides’, are one of the most obvious signs of eutrophication in coastal and estuarine areas, worldwide, and in Northwestern France in particular. The processes underlying macroalgal proliferations are almost identified whereas their ecological consequences - especially for ichthyofauna - remain poorly understood and understudied. The objective of this thesis is to characterise the effects of green tides on the role of coastal and estuarine habitats for ichthyofauna, on both the community and the individual scales.First, we investigated the consequences of green tides on ichthyofauna on the community scale. Then, we examined the processes which affect the ichthyofauna during green tides, on the individual scale. Marine juvenile fish responses were investigated based on habitat selection and comparison of individual performances between a control and an impacted site. Based on these two complementary approaches, we evidence ecological significant negative effects of green tides on the quality of essential fish habitats. The effects of green tides on ichthyofauna are modulated by the intensity and nature of macroalgal blooms, and are related to the fish habitat use and ecology. A decreasing gradient of sensibility to green tides is emphasised, from benthic to demersal and pelagi

This diploma thesis deals with the design of the kindergarten in Uničov. The object is designed as a two-storey, non-cellar basement. Because of the almost perfectly flat terrain and purpose of the building, the basement would be unnecessary. The gentle slope which the selected plot shows is oriented to the southeast side. The main entrance to the house is solved from the western side, along the direction of the main entrance to the nursery. It is adjacent to the existing communication with the parking lot towards the Nemocniční housing estate. There are two separate children's wards at the 1st floor, always equipped with a spacious playroom, dressing rooms and a washroom. Furthermore, in the 1st floor there is a large part of the economic zone consisting of a kitchen and related space, a junior flat and a cookery facility. The 2nd floor is reserved for the all-round development of children, both directly attending kindergarten and outside of the interest groups. Therefore, there are spaces such as a language classroom, a workshop room and a hall for other external staff. In addition, there is also a facility for staff and external staff in the form of a meeting room. The east side of the 2nd floor is then reserved for the third children's ward, the backrest consisting of a playroom, dressing rooms, washrooms along with a toilet and, moreover, a lounger. The vertical supporting structures are designed from porous concrete blocks, reinforced concrete and pillar blocks. The ceiling in each level is designed as a reinforced concrete reinforced concrete slab. The building is roofed with a flat single-roofed roof with a slope of 2 %.

The theme of my diploma thesis is processing of project documentation for building a construction of nursery school in Kunštát. Health centre was also designed as a part of the building facility. The capacity of the building are two classrooms in nursery school and two outpatient units. The building is a two-storey building which has basement partly under it. The construction system is bricked from aerated concrete blocks with a ceiling structure made of reinforced concrete prestressed ceiling panels. The perimeter masonry is insulated with an ETICS thermal insulation composite system with mineral wool thermal insulation. The predominant part of the roof of the building is a vegetation extension flat roof.

The Master’s thesis deals with the business plan to found a private kindergarten in the town of Rájec-Jestřebí. The kindergarten will be established as a limited liability company. The thesis consists of three basic parts. The introduction describes the theoretical bases.The second part deals with analyses of the currentmarket situation. The final part contains the specific business plan, which is created on the basis of market gaps in this area in the market.

This master´s thesis is focused on suitable design of a new nursery school building. Nursery school is situated in suburban area, and as such two level design was chosen. The building has U shape outline. Ground floor is intended for basic operation of the nursery school, while the second floor is designed to accommodate various extra-curricular activities. Foundations of the building are made of plain concrete. Porotherm system is used for all walling. Ceilings are made of precast hollow core slabs - Spiroll. Object is covered with a flat roof.

The aim of this thesis was in the range of implement documentation with all the necessary reports and calculations. The project involves the new building nursery school in Bílovice nad Svitavou. Project was created with modern computer applications. This thesis has been processed in accordance with applicable rules and regulations. The thesis is arranged in three parts, separate architectural study, text attachments and drawing part.

The master thesis with the name Nursery school in Sedlejov solves project documentation in the degree in building construction. Nursery school has two floors. The object is without a cellar. As roofing is used a flat roof. Walling system is called POROTHERM with contact insulation from mineral cotton wool.