Upon ageing, a progressive disruption of protein homeostasis often leads to extensive protein aggregation and neurodegeneration. It is therefore important to study at the proteome level the origins and consequences of such disruption, which so far have remained elusive. Addressing this problem has recently become possible by major advances in mass spectrometry-based (MS) proteomics, which allows the identifications and quantification of thousands of proteins in a variety of biological samples. In the first part of this thesis, I analyse proteome-wide MS data for the nematode worm C. elegans upon ageing, in wild type (WT), long-lived and short-lived mutant strains. By comparing the total abundance and the soluble abundance for nearly 4000 proteins, I provide extensive evidence that proteins are expressed in adult worms at levels close to their solubility limits. With the use of sequence-based prediction tools, I then identify specific physico-chemical properties associated with this age-related protein homeostasis impairment. The results that I obtained reveal that the total intracellular protein content remains constant, in spite of the fact that the proteome undergoes wide remodeling upon ageing, resulting into severe protein homeostasis disruption and widespread protein aggregation. These results suggest a protein-dependent decrease in solubility associated with the protein homeostasis failure. In the second part of the thesis, I determine and classify potential interactions of misfolded protein oligomers with other proteins. This phenomenon is widely believed to give rise to cytotoxicity, although the mechanisms by which this happens are not fully understood. To address this question, I process and analyse MS data from structurally different oligomers (toxic type A and nontoxic type B) of the protein HypF-N, incubated in vitro with proteins extracted from murine cell cultures. I find that more than 2500 proteins are pulled down with the misfolded oligomers. These results indicate that the two types of oligomers interact with the same pool of proteins and differ only in the degree of binding. Functional annotation analysis on the groups reveals a preference of the oligomers to bind proteins in specific biological pathways and categories, including in particular mitochondrial membrane proteins, RNA-binding proteins and molecular chaperones. Overall, in this study I complement the powerful and high-throughput experimental approach of MS proteomics with bioinformatics analyses and prediction algorithms to define the physical, chemical and biological features of protein homeostasis disruption upon ageing and the interactome of misfolded oligomers.

Conselho Nacional de Desenvolvimento Científico e Tecnológico / The impact of retorted oil shale (ROS) addition on the dynamics of carbon (C) of crop residues (CR) in the soil is little known. Under laboratory conditions, the present study aimed to evaluate the residual and immediate effects of ROS on C mineralization of CR, water stability aggregates and storage C in the particulate (>53 μm) and associated minerals fractions (<53μm) of organic matter (OM) of a Hapludalf. In experiment 1, treatments consisted of soil samples from plots that received four additions of ROS, totaling 0, 6,000 and 12,000 kg ha-1. In experiment 2, treatments consisted of soil with no history of application of ROS (0 kg ha-1), that in the laboratory received three doses of ROS (0, 1,500 and 3,000 kg ha-1). In both experiments, the treatments were evaluated in the presence (3,000 kg ha-1) and absence of leaves and stalks of soybeans. The release of C-CO2 was evaluated for 80 days and at the end of the experiment determined the aggregate stability and C storage in different OM fractions. The addition of CR in soil with a history of ROS addition did not alter the release of C-CO2 and also did not reduce the apparent mineralization of C of RC compared to addition of these residues in soil with no history. In experiment 2, there was a reduction in the release of C-CO2 compared to the control only the treatment with stalks + 3000 kg ROS ha-1. In experiment 2, the treatment stalks + 3000 kg ROS ha-1 provided an increase in aggregate stability compared with treatment with only stalks. In experiment 1, the ROS addition tended to higher retention of C stalks and leaves in the soil. In experiment 2, the application of CR + ROS promoted greater retention of soil C only in the treatment with leaves. Under laboratory conditions, the soil with a history of ROS addition did not affect mineralization and retention of crop residue C added to soil. Besides, the ROS showed an immediate effect, reducing C mineralization from stalks and increasing retention of C from leaves added to the soil. / O impacto da adição de xisto retortado (XR) sobre a dinâmica do carbono (C) de resíduos culturais (RC) no solo é pouco conhecido. Em condições de laboratório, o presente estudo objetivou avaliar o efeito residual e imediato do XR sobre a mineralização do C de RC, estabilidade de agregados em água e armazenamento de C nas frações particulada (>53 μm) e associada aos minerais (<53 μm) da matéria orgânica (MO) de um Argissolo Vermelho Distrófico arênico. No experimento 1, os tratamentos foram compostos por amostras de solo de parcelas que receberam quatro aplicações de XR, totalizando 0, 6.000 e 12.000 kg ha-1. Já no experimento 2, os tratamentos foram constituídos com solo somente da parcela sem histórico de aplicação de XR (0 kg ha-1), que no laboratório recebeu três doses de XR equivalentes a 0, 1.500 e 3.000 kg ha-1. Em ambos os experimentos os tratamentos foram avaliados na presença (3.000 kg ha-1) e ausência de folhas e talos de soja. A liberação de C-CO2 foi avaliada durante 80 dias e ao final do experimento determinados a estabilidade de agregados e o armazenamento de C nas diferentes frações da MO. A adição de RC no solo com histórico de adição de XR não alterou a liberação de C-CO2 e também não reduziu a mineralização aparente do C dos RC comparado a adição desses resíduos em solo sem histórico. No experimento 2, houve redução na liberação de C-CO2 em relação ao controle somente no tratamento com talos + 3.000 kg de XR ha-1. No experimento 2, o tratamento talos + 3.000 kg de XR ha-1 proporcionou incremento na estabilidade de agregados em comparação com o tratamento somente com talos. No experimento 1, a adição de XR apresentou tendência de maior retenção de C dos talos e folhas no solo. No experimento 2, a aplicação conjunta de resíduos e XR promoveu maior retenção de C no solo apenas no tratamento com folhas. Em condições de laboratório, o solo com histórico de aplicação de XR não afetou a mineralização e a retenção do C de resíduos culturais adicionados ao solo. Além disso, o XR apresentou efeito imediato, reduzindo a mineralização do C de talos e aumentando a retenção do C de folhas adicionadas ao solo.

In some species, including humans, red blood cells (RBCs) under low shear stress tend to clump together and form into regular stacks called rouleaux. These stacks are not static, and constantly move and break apart. This phenomenon is referred to as red blood cell aggregation and disaggregation. When modelled as a single liquid, blood behaves as a non-Newtonian fluid. Its viscosity varies, mainly due to the aggregation of RBCs. The aim of this research is to develop a mesoscale computational model for the simulation of RBCs in plasma. This model considers RBC interaction and aggregation to predict blood-flow characteristics such as viscosity, rouleaux size and velocity distribution. In this work, the population-balance modelling (PBM) approach is utilized to model the RBC aggregation process. The PBM approach is a known method that is used for modelling agglomeration and breakage in two-phase flow fluid mechanics to find aggregate size. The PBM model is coupled to the incompressible Navier-Stokes equations for the plasma. Both models are numerically solved simultaneously. The population-balance equation has been used previously in a more restricted form, the Smoluchowski equation, to model blood viscosity, but it has never been fully coupled with the Navier-Stokes equation directly for the numerical modelling of blood flow. This approach results in a comprehensive model which aims to predict RBC aggregate size and their velocities for different flow configurations, as well as their effects on the apparent macro-scale viscosity. The PBM approach does not treat the microscopic physics of aggregation directly but rather uses experimental correlations for aggregation and disaggregation rates to account for the effects of aggregation on the bulk. To find the aggregation rate, a series of experiments on RBC sedimentation due to gravity is designed. In these tests, aggregated RBCs (rouleaux) tend to settle faster than single RBCs and, due to low shear stresses, disaggregation is very low and can be neglected. A high-speed camera is used to acquire video-microscopic pictures of the process. The size of the aggregates and their velocities are extracted using image processing techniques. For image processing, a general Matlab program is developed which can analyze all the images and report the velocity and size distribution of rouleaux. An experimental correlation for disaggregation rate is found using results from a previous steady-state Couette flow experiment. Aggregation and disaggregation rates from these experiments are used to complete the PBM model. Pressure-driven channel flow experiments are then used for the final validation of the model. Comparisons of the apparent viscosity of whole blood in previous experiments show reasonable agreement with the developed model. This model fills a gap between micro-scale and macro-scale treatments and should be more accurate than traditional macro-scale models while being cheaper than direct treatment of RBCs at the micro-scale.

Nos últimos anos, complexos de irídio tem sido sugeridos para uso em materiais luminescentes, tais como diodos orgânicos emissores de luz (OLED\'s) e células eletroquímicas emissoras de luz (LEEC\'s). Suas potenciais utilidades como dispositivo é devido as suas características físico-químicas e fotofísicas, as quais são caracterizadas por tempos de vida curtos para o tripleto, estabilidade térmica, além da possibilidade de modificar a emissão sobre uma vasta gama de cores do espectro. Atualmente, muitas pesquisas tentam encontrar complexos de irídio com emissão de luz azul. No entanto, embora emissores de luz azul já tenham sido desenvolvidos, existe o problema relacionado a agregação destes complexos. Para resolver este o problema da agregação é necessário modificar a estrutura padrão do complexo com cadeias carbônicas saturadas para evitar empilhamento &pi;-&pi;. Experimentalmente esta é uma tarefa um tanto difícil. Dessa forma, métodos computacionais têm sido viáveis como uma abordagem para entender a estrutura e propriedades eletrônicas dos sistemas estudados. Aqui, é apresentado um estudo teórico baseado na teoria do funcional da densidade (DFT) para investigar a agregação de complexos de irídio, além de predizer como esta pode ser controlada com o uso de grupos substituintes adequados. Os cálculos foram realizados usando funcional PBE0 e conjunto de base 6-31G*, o qual provou ser adequada na descrição das propriedades do complexo. Portanto, cálculos subsequentes mostraram bons resultados, onde os estados excitados foram previstos ser de natureza predominantemente MLCT (transferência de carga do metal para o ligante) para o complexo 1 e LC (carga centrada no ligante) para o complexo 2. Alterações no ambiente químico provou ter grande influência sobre os estados excitados, onde a inclusão do solvente favoreceu a estabilidade, mantendo os estados excitados tripletos com energia entre 3.01 eV e 3.03 eV. Além disso, o uso dos grupos substituintes provou ser de grande importância para prevenir a agregação, especialmente no complexo 2, em que a partir do dímero matriz (sem substituintes) para o complexo substituído 2-1 (com metil) houve uma desestabilização da energia de interação entre os monômeros no dímero por ~ 19.78 kcal/mol, sendo que a energia do primeiro foi estimada ser -39.78 kcal/mol, enquanto o segundo foi -20 kcal/mol. Nossos resultados sugerem que a modificação do complexo torna-se bastante promissora, fazendo uso de pequenos grupos substituintes e cadeias carbônicas alifáticas, evitando assim, a agregação por empilhamento &pi;-&pi;. / In the last few years, iridium complexes have been suggested for use in luminescent materials such as organic light-emitting diodes (OLEDs) and light emitting electrochemical cells (LEECs)1-3. Their potential utility as luminescent devices is due to the physicochemical and photophysical properties, which are featured by short triplet emitting lifetimes (microseconds time scale), thermal stability, besides of possibility to tune the emission over desired spectral range. Actually, many researches try to find iridium complexes with efficient deep-blue emission, in order to use in these devices. Although many deep-blue emitters have been developed, there is still a big problem related to aggregation. So far, little has been reported about iridium complexes in solid environments, such as inorganic matrix of OLEDs and LEECs devices. To solve this issue is needed tune the structure of the complex in order to prevent the aggregation (steric hindrance), by means of drawing saturated carbon chains on the ligands to avoid the &pi;-&pi; stacking. Experimentally, this is a rather challenging and expensive task. In this aspect, computational methods have been performed as approaches to gain deeper insights about the structure and electronic properties of the studied systems. Here, we present a theoretical study based on density functional theory to investigate the aggregation iridium complexes, in addition to predict how this can be controlled with the use of suitable substituent groups. Calculations were performed using functional PBE0 and 6-31G* basis functions, which proved adequate in describing the properties of the complex. Therefore, subsequent calculations showed good results, where the excited state of the complex are provided, these being predominantly MLCT (metal to ligand charge transfer) nature for the complex 1 and LC (ligand centered) nature to complex 2. Changes in the chemical environment proved to have great influence on the excited states, where the inclusion of the solvent favored its stability, keeping the triplet excited states with energy between 3.01 and 3.03 eV. Furthermore, the use of substituent groups proved to be of great importance to prevent aggregation, especially in complex 2, wherein from the matrix dimer (no substituted) to complex with methyl groups there was a destabilization of the interaction energy between the monomers in the dimer by ~ 19.78 kcal / mol, being that the energy of the first was -39.78 kcal / mol, while the second was to -20 kcal / mol. Our results suggest that the modification of the complex becomes quite promising, making use of small groups and aliphatic carbon chains, thus avoiding aggregation by stacking.

L'analyse des systèmes de grande taille est confrontée à des difficultés d'ordre syntaxique et sémantique : comment observer un million d'entités distribuées et asynchrones ? Comment interpréter le désordre résultant de l'observation microscopique de ces entités ? Comment produire et manipuler des abstractions pertinentes pour l'analyse macroscopique des systèmes ? Face à l'échec de l'approche analytique, le concept d'émergence épistémique - relatif à la nature de la connaissance - nous permet de définir une stratégie d'analyse alternative, motivée par le constat suivant : l'activité scientifique repose sur des processus d'abstraction fournissant des éléments de description macroscopique pour aborder la complexité des systèmes. Cette thèse s'intéresse plus particulièrement à la production d'abstractions spatiales et temporelles par agrégation de données. Afin d'engendrer des représentations exploitables lors du passage à l'échelle, il apparaît nécessaire de contrôler deux aspects essentiels du processus d'abstraction. Premièrement, la complexité et le contenu informationnel des représentations macroscopiques doivent être conjointement optimisés afin de préserver les détails pertinents pour l'observateur, tout en minimisant le coût de l'analyse. Nous proposons des mesures de qualité (critères internes) permettant d'évaluer, de comparer et de sélectionner les représentations en fonction du contexte et des objectifs de l'analyse. Deuxièmement, afin de conserver leur pouvoir explicatif, les abstractions engendrées doivent être cohérentes avec les connaissances mobilisées par l'observateur lors de l'analyse. Nous proposons d'utiliser les propriétés organisationnelles, structurelles et topologiques du système (critères externes) pour contraindre le processus d'agrégation et pour engendrer des représentations viables sur les plans syntaxique et sémantique. Par conséquent, l'automatisation du processus d'agrégation nécessite de résoudre un problème d'optimisation sous contraintes. Nous proposons dans cette thèse un algorithme de résolution générique, s'adaptant aux critères formulés par l'observateur. De plus, nous montrons que la complexité de ce problème d'optimisation dépend directement de ces critères. L'approche macroscopique défendue dans cette thèse est évaluée sur deux classes de systèmes. Premièrement, le processus d'agrégation est appliqué à la visualisation d'applications parallèles de grande taille pour l'analyse de performance. Il permet de détecter les anomalies présentes à plusieurs niveaux de granularité dans les traces d'exécution et d'expliquer ces anomalies à partir des propriétés syntaxiques du système. Deuxièmement, le processus est appliqué à l'agrégation de données médiatiques pour l'analyse des relations internationales. L'agrégation géographique et temporelle de l'attention médiatique permet de définir des évènements macroscopiques pertinents sur le plan sémantique pour l'analyse du système international. Pour autant, nous pensons que l'approche et les outils présentés dans cette thèse peuvent être généralisés à de nombreux autres domaines d'application. / The analysis of large-scale systems faces syntactic and semantic difficulties: How to observe millions of distributed and asynchronous entities? How to interpret the disorder that results from the microscopic observation of such entities? How to produce and handle relevant abstractions for the systems' macroscopic analysis? Faced with the failure of the analytic approach, the concept of epistemic emergence - related to the nature of knowledge - allows us to define an alternative strategy. This strategy is motivated by the observation that scientific activity relies on abstraction processes that provide macroscopic descriptions to broach the systems' complexity. This thesis is more specifically interested in the production of spatial and temporal abstractions through data aggregation. In order to generate scalable representations, the control of two essential aspects of the aggregation process is necessary. Firstly, the complexity and the information content of macroscopic representations should be jointly optimized in order to preserve the relevant details for the observer, while minimizing the cost of the analysis. We propose several measures of quality (internal criteria) to evaluate, compare and select the representations depending on the context and the objectives of the analysis. Secondly, in order to preserve their explanatory power, the generated abstractions should be consistent with the background knowledge exploited by the observer for the analysis. We propose to exploit the systems' organisational, structural and topological properties (external criteria) to constrain the aggregation process and to generate syntactically and semantically consistent representations. Consequently, the automation of the aggregation process requires solving a constrained optimization problem. We propose a generic algorithm that adapts to the criteria expressed by the observer. Furthermore, we show that the complexity of this optimization problem directly depend on these criteria. The macroscopic approach supported by this thesis is evaluated on two classes of systems. Firstly, the aggregation process is applied to the visualisation of large-scale distributed applications for performance analysis. It allows the detection of anomalies at several scales in the execution traces and the explanation of these anomalies according to the system syntactic properties. Secondly, the process is applied to the aggregation of news for the analysis of international relations. The geographical and temporal aggregation of media attention allows the definition of semantically consistent macroscopic events for the analysis of the international system. Furthermore, we believe that the approach and the tools presented in this thesis can be extended to a wider class of application domains.

Made available in DSpace on 2014-06-11T19:28:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-24Bitstream added on 2014-06-13T18:34:42Z : No. of bitstreams: 1 martins_mr_me_jabo.pdf: 875154 bytes, checksum: 45796fe2b99e06de29ac7820c0a96c73 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A adaptação do sistema de semeadura direta (SSD) depende da escolha adequada da seqüência de culturas, que devem contribuir para melhorar os atributos solo. O objetivo do presente trabalho foi avaliar o efeito de seqüências de culturas na agregação do solo e no teor de carbono orgânico e polissacarídeos em diferentes classes de agregados estáveis em água de um Latossolo Vermelho eutrófico sob SSD. Um experimento foi implantado em 2002 em Jaboticabal, SP. Os tratamentos foram constituídos pela combinação de quatro seqüências de culturas de verão e sete culturas de inverno. As seqüências de culturas de verão, semeadas em outubro/novembro, foram: monocultura de milho; monocultura de soja; cultivos intercalados ano a ano de soja e milho; seqüência de cultivos de arroz/feijão/algodão/feijão. As culturas de inverno, semeadas em fevereiro/março, repetidas todos os anos nas mesmas parcelas, foram: milho, girassol, nabo forrageiro, milheto, feijão guandu, sorgo granífero e crotalária. A amostragem do solo foi realizada após o quarto ano de condução do experimento, em outubro de 2006. O cultivo de milho em monocultura no verão favoreceu a formação de agregados estáveis em água com diâmetro entre 6,30–2,00 mm e proporcionou o maior teor de COT e PAD nessa classe de tamanho de agregados. Isso indica que a influência das culturas sobre a estabilidade de agregados foi intermediada pelos teores de COT e PAD. Não foi verificada diferença na agregação do solo entre culturas de inverno utilizadas. Os maiores teores de COT, PST e PAD foram verificados nos agregados com diâmetro entre 2,00–1,00 mm e os menores teores nos agregados <0,25 mm. / A better performance of the no-tillage system in tropical regions depends on the choice of suitable crop sequences in summer and winter. These crops should contribute to improvement of soil properties. The objective of this work was to assess crop sequences effects on soil aggregation and organic carbon and polysaccharide contents in water-stable aggregate size classes of a Rhodic Oxisol under no-tillage. An experiment was established in Jaboticabal town, São Paulo state, in 2002. Treatments were constituted for a combination of four crop sequences in summer and seven crop sequences in the winter. Crop sequences in the summer were: corn monoculture (CC); soybean monoculture (SS); soybean/corn/soybean/corn sequence (SC) and rice/bean/cotton/bean sequence (RB), seeded in October/November. Winter crops were: corn, sunflower, oilseed radish, millet, pigeonpea, sorghum and sunn hemp, seeded in February/March. Soil sampling took place after forth year after experiment implantation, in October 2006. The MV sequence in summer increased the percentage of 6,30–2,00 mm water-stable aggregates and provided the highest total organic carbon and diluted-acid-extractable polysaccharides contents in the same aggregate size class. These results suggest that crop effects on soil aggregate stability can be mediated by total organic carbon and diluted-acid-extractable polysaccharides. The winter crops do not influence soil aggregation. The highest and lowest total organic carbon, total polysaccharides and diluted-acid-extractable polysaccharides contents was verified, respectively, in 2,00–1,00 mm and <0,25 mm water-aggregate soil size classes.

Made available in DSpace on 2014-06-11T19:23:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-02Bitstream added on 2014-06-13T20:29:42Z : No. of bitstreams: 1 freitas_dv_me_rcla.pdf: 971560 bytes, checksum: 12bf77581a5c1c40a7b070f7afebb636 (MD5) / Estudos abrangendo aspectos da ecologia populacional de plantas fornecem informações importantes sobre os processos de regeneração, ocorrência de perturbações e os meios pelos quais as espécies exploram seus ambientes. Estudos dessa natureza em Florestas de Restinga, feições marcantes no litoral brasileiro, ainda são escassos. Assim, no presente trabalho foram investigados alguns aspectos da ecologia populacional de Calophyllum brasiliense Cambess. (Clusiaceae), uma espécie de ampla ocorrência em ambienteis sujeitos à inundação nas florestas Neotropicais, em 0,5 ha de Floresta de Restinga, localizada no Parque Estadual da Serra do Mar, Núcleo Picinguaba, Ubatuba, SP. Foi realizada uma análise temporal da estrutura de tamanho e do padrão de distribuição espacial da população, visando compreender como a heterogeneidade ambiental, relacionada às variações na microtopografia (mosaico de regiões sobre e entre cordões arenosos), na abertura do dossel da floresta e nas condições de drenagem do solo, afeta os parâmetros da estrutura populacional analisados. Observou-se que a população apresenta estrutura com predomínio de indivíduos nas menores classes de tamanho e distribuição agregada em todas as classes (plântulas, jovens, imaturos e adultos). A saturação hídrica do solo e as diferenças na microtopografia dos cordões arenosos influenciaram o padrão encontrado e afetaram os parâmetros demográficos, entretanto, não foram encontradas correlações entre a abertura de dossel e os parâmetros analisados. Além disso, a análise da estrutura de tamanho em subclasses de altura revelou ausência de indivíduos com alturas entre três e 15 metros, o que pode ser um indicativo de que apesar da população apresentar representantes em todas as classes de tamanho, o acesso dos indivíduos à classe reprodutiva está comprometido... / Studies concerning plant population ecology provide important information about status of regeneration, occurrence of disturbances and the means by which the species exploit the environment. Studies like these, in the “Restingas” forests, striking features in the Brazilian coast, are still scarce. Therefore, we investigated some aspects of population ecology of Calophyllum brasiliense Cambess. (Clusiaceae), in 0.5 ha of the Restinga Forest, in the “Parque Estadual da Serra do Mar”, “Núcleo Picinguaba”, Municipality of Ubatuba, São Paulo State. We performed a temporal analysis of size structure and spatial pattern, to understand how the environmental heterogeneity, such as microtopography variations, light availability and soil drainage conditions can affect the population structure. Population structure presented dominance of individuals in lower size classes as well as aggregated distribution. These patterns result of both drainage and microtopography conditions, however, no correlations were found between canopy openness and the parameters analyzed. In addition, the size structure analysis, performed into different height classes, showed no individuals with heights between 3 and 15 m, which can suggest that the access to reproductive class seems to be compromised, even though there were individuals within all size classes. It was also observed a high frequency of herbivory damage; however, such damage did not contribute to increase the mortality rate of the population. In general, it could be concluded that environmental heterogeneity has a major influence on the size structure and spatial distribution, mainly in smaller size classes, and that the population seems to have trouble reaching the reproductive stage, which can lead, as a long term, to its disappearance in the area.

The thesis objective was to evaluate the forms of accumulation and release of phosphorus from cover crops waste under long time of different soil management systems and its relationship with the soil phosphorus forms. The experiment was established in 1986 on an Oxisol in the IAPAR experimental station in Pato Branco, Paraná State, Brazil. The treatments consisted of six winter cover crops (blue lupine, vetch, oats, radish, rye and wheat) and the winter fallow treatment, cultivated under no-tillage (NT) and conventional (CT). In September 2011 were collected soil samples (0-5 and 5-10 cm) and the shoots of winter cover crops. Confected litterbags (0.2 x 0.2 m) were collected at 7, 14, 28, 56 and 112 days after the cover crops management. In the residues collected, we assessed the accumulation of C, N, P, and held the fractionation of P. In the soil samples, we made the separation of aggregates by wet way and in the original soil and aggregates we evaluated the content of total C, N, P, the P forms, and the acid phosphatase activity. The distribution of P forms in the cover crops tissue varies among species and their accumulation was a function of nutrient soil P availability, as in NT. But for all of them, the soluble inorganic P was the principal storage form. The release of P from plant residues depends not only on its rate of decomposition, but also on the content of total and soluble P. The vetch and rye have great potential to recycle soil P, and this amount of P should be considered when prescribing phosphorus fertilization for the culture implanted in sequence. However, the long term cultivation of different winter cover crops did not affected the soil P forms in highly weathered clay soils, managed under NT and CT. On the other hand, practices that favor the accumulation of organic residues on the soil surface as the NT system, increase the acid phosphatase enzyme activity and labile organic and inorganic P forms, besides the formation of larger aggregates, which have higher levels of C, N, total P and organic labile P. / O objetivo desta tese foi avaliar as formas de acúmulo e liberação de fósforo dos resíduos de plantas de cobertura sob diferentes sistemas de manejo de solo por longa duração e sua relação com as formas de fósforo no solo. O experimento foi instalado em 1986 sobre um Latossolo Vermelho distroférrico da estação experimental do IAPAR em Pato Branco, estado do Paraná. Os tratamentos consistem em seis plantas de cobertura de inverno (tremoço azul, ervilhaca comum, aveia preta, nabo forrageiro, centeio e trigo) mais uma parcela que permanece em pousio no período de inverno, manejadas sob sistema plantio direto (SPD) e sistema de cultivo convencional (SCC). Em setembro de 2011, coletaram-se amostras de solo (0-5 e 5-10 cm) e da parte aérea das plantas de cobertura de inverno. Confeccionaram-se bolsas de decomposição (0,2 x 0,2m), que foram coletadas aos 7, 14, 28, 56 e 112 dias após o manejo das plantas de cobertura. Nos resíduos coletados, avaliou-se o acúmulo de C, N, P e o realizou-se o fracionamento do P. Nas amostras de solo, realizou-se a separação de agregados por via úmida e, no solo original e nos agregados, realizou-se a avaliação dos teores de C, N, P e o fracionamento de P, e da atividade de fosfatase ácida. A distribuição das formas de P no tecido das plantas de cobertura varia entre as espécies e a sua acumulação foi função da disponibilidade de P no solo. Mas, para todas elas, o P inorgânico solúvel foi a principal forma de armazenamento. A liberação de P dos resíduos das plantas de cobertura não depende apenas da sua taxa de decomposição, mas também do teor total e solúvel de P. A ervilhaca comum e o centeio possuem grande potencial para reciclar P do solo, sendo que essa quantidade de P deve ser considerada na prescrição da adubação fosfatada da cultura implantada em sequência. Contudo, o cultivo de plantas de cobertura de inverno por longo período não alterou as formas de P em solo argiloso altamente intemperizado, manejado sob SPD e sob SCC. Por outro lado, práticas que propiciem o acúmulo de resíduos orgânicos na superfície do solo, como o SPD, elevam a atividade de fosfatase ácida e as formas lábeis orgânicas e inorgânicas de P, além da formação de agregados maiores, os quais apresentam teores mais elevados de C, N, P total e P orgânico lábil.

Submitted by Maike Costa (maiksebas@gmail.com) on 2017-01-31T12:37:20Z No. of bitstreams: 1 arquivototal.pdf: 2719783 bytes, checksum: 00456e5b8ca69f853baec641a28cd6f2 (MD5) / Made available in DSpace on 2017-01-31T12:37:20Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2719783 bytes, checksum: 00456e5b8ca69f853baec641a28cd6f2 (MD5) Previous issue date: 2016-04-01 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The β-D-glucans are polysaccharides constituting the cell wall of yeasts such as Saccharomyces cerevisiae. Evidence shows polymers have several beneficial effects, mainly related to immunomodulation. However, their action on platelet and endothelial function is understudied. This study aimed to evaluate the effect of (1-3) (1-6) β-D-glucan extracted from S. cerevisiae and derivative carboxymethylated (CM-G) on vascular and platelet function in rats. The animals were treated orally with CM-G and BG-Sc at a dose of 20 mg/kg daily for eight days and the controls received saline as placebo. At end, were collected blood and thoracic aortic artery to study platelet activation and aggregation by light transmission, flow cytometry, vascular reactivity and cytokine assay. In vitro studies of platelet activation and aggregation induced by adenosine diphosphate (ADP) and collagen were conducted with CM-G at different concentrations (100 and 300 μg/mL). Significant reduction in IL-8 levels (4,18 ± 0,72 pg/mL) in the group treated with CM-G was also found when compared to control (22,7 ± 6,9 pg/mL) and the BG-Sc group (16,4 ± 2,4 pg/mL). The reactivity studies shows BG-Sc and CM-G had no influence on vascular response to phenylephrine (PHE) compared to the control. In the group treated with BG-SC, the response to vasorelaxantes agents such as acetylcholine (ACH; Emax = 90,2 ± 14,1%; pD2 = 6,36 ± 0,30 M) and sodium nitroprusside (SNP; Emax = 92,3 ± 2,4%; pD2 = 10,21 ± 0,10M) were impaired compared to controls (ACH: Emax = 100 ± 7,5%; pD2 = 8,17 ± 0,25 M; SNP: Emax = 100 ± 9,5%; pD2 = 11,18 ± 0,27 M). However, treatment with CM-G (max = 93,1 ± 2,7%; pD2 = 11,71 ± 0,07 M) increased the potency of NPS when compared with the control (Emax = 100 ± 9.5%; pD2 = 11.18 ± 0.27). In vitro studies of platelet aggregation by light transmission reveals CM-G inhibited the aggregation stimulated by ADP in doses of 100 and 300 mg/ml, reaching 25,7 ± 2,7% and 14,8 ± 3,2% of aggregation, respectively. When aggregation was induced by collagen, only the dose 300 mg/ml had inhibitory activity (33 ± 6,2%). The antiplatelet effect was similar to acetylsalicylic acid when the aggregation was generate by ADP. Aggregation inhibition was also demonstrated by flow cytometry, however was observed that CM-G had no effect on platelet activation. In treated animals, there was inhibition of platelet aggregation induced by ADP (45% ± 3,9 and 45 ± 6%, respectively). However, when induced by collagen, antiplatelet effect was observed only in animals receiving the BG-Sc (22,6 ± 7,7%). The treatment and in vitro aggregation assays suggest that CM-G appears to be more selective for ADP. The findings indicate the CM-G and BG-Sc feature antiplatelet effect and modulating vascular function. Thus, the use of these polysaccharides may be a possible tool for the prevention of cardiovascular diseases / As β-D-glucanas são polissacarídeos constituintes da parede celular de leveduras, como a Saccharomyces Cerevisae. Evidências mostram que esses polímeros possuem diversos efeitos benéficos, principalmente relacionados a imunomodulação. Entretanto, a sua ação sobre a função plaquetária e endotelial é pouco estudada. Assim, este estudo teve como objetivo avaliar o efeito da (1-3) (1-6) β-d-glucana extraída de S. cerevisae e do seu derivado carboximetilado (CM-G) sobre a função vascular e plaquetária em ratos. Os animais foram tratados por via oral com CM-G e BG-Sc na dose de 20 mg/Kg, diariamente, durante oito dias, e os controles receberam solução salina como placebo. Ao final, foram coletados o sangue e a artéria aorta torácica para estudos de agregação e ativação plaquetária por transmissão luminosa, citometria de fluxo, reatividade vascular e dosagem de citocinas. Foram realizados estudos in vitro de agregação e ativação plaquetária induzida por adenosina difosfato (ADP) e colágeno com a CM-G em diferentes concentrações (100 e 300 μg/mL). Constatou-se redução significativa dos níveis de IL-8 (4,18±0,72 pg/mL) no grupo tratado com CM-G ao se comparar com o grupo controle (22,7±6,9 pg/mL) e com o grupo BG-Sc (16,4±2,4 pg/mL). Os estudos de reatividade mostraram que a BG-Sc e a CM-G não exerceram influência sobre a resposta vascular da fenilefrina (PHE) em relação ao controle. No grupo tratado com BG-SC, a resposta aos agentes vasorelaxantes acetilcolina (ACH; Emax = 90,2 ± 14,1%; pD2 = 6,36 ± 0,30 M) e nitroprussiato de sódio (NPS; Emax = 92,3± 2,4%; pD2 = 10,21 ± 0,10M) foram prejudicadas em relação aos controles (ACH: Emax = 100 ± 7,5%; pD2 = 8,17 ± 0,25 M; NPS: Emax = 100 ± 9,5%; pD2 = 11,18 ± 0,27 M). Porém, o tratamento com a CM-G (Emax = 93,1 ± 2,7 %; pD2 = 11,71 ± 0,07 M) aumentou a potência do NPS quando comparado com o controle (Emax = 100 ± 9,5%; pD2 = 11,18 ± 0,27 M). Os estudos de agregação plaquetária in vitro por transmissão luminosa revelam que a CM-G inibiu a agregação estimulada por ADP nas doses de 100 e 300 μg/mL, atingindo 25.7±2.7% e 14.8±3.2% de agregação, respectivamente. Quando a agregação foi induzida pelo colágeno, apenas a dose 300 μg/mL teve ação inibitória (33 ± 6,2%). O efeito antiagregante foi similar ao ácido acetilsalicílico quando a agregação foi induzida por ADP. Inibição da agregação também foi demonstrada na técnica de citometria de fluxo, porém observou-se que a CM-G não afetou a ativação plaquetária. Nos animais tratados, verificou-se inibição da agregação plaquetária induzida por ADP (45 ± 3.9% e 45 ± 6 %, respectivamente). Entretanto, quando induzido por colágeno, o efeito antiagregante foi visto apenas nos animais que receberam BG-Sc (22.6 ± 7.7%). O tratamento e os ensaios de agregação in vitro sugerem que a CM-G parece ser mais seletiva para o ADP. Os achados indicam que a CM-G e a BG-Sc apresentam efeito antiagregante e moduladora da função vascular. Assim, o uso desses polissacarídeos pode ser uma possível ferramenta para a prevenção de doenças cardiovasculares.

Made available in DSpace on 2015-05-14T12:09:41Z (GMT). No. of bitstreams: 1 parte1.pdf: 1889710 bytes, checksum: a173fa12e136e0893e402eea33c32629 (MD5) Previous issue date: 2008-09-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This work analyzes the constructive actuations of the Real Estate Portfolio of Montepio Paraiba State in the statal housing production in the city of João Pessoa, from 1932 to 1963, established between the institutional designation for the production of housing in benefit of the public functionalism and its last popular realization before the work of BNH. Through exhausting documental research, done in local collections and especially through the newspaper A União , official record of the realizations of the state executive, data was found regarding the realizations of the housings by the institution, identifying the groups of popular housing and later on classifying the built unities and the reconstitution of the house plans and the front elevation. And through the study of the introduction of these popular units and of most of the residential housings, as were called the houses that were built separately and that were not considered popular, we managed to determine the relation between the built patrimony by Montepio and the urban evolution of João Pessoa. And even without grand urbanistic plans or big aggregations as other federal institutions, the Montepio consolidated the residential use in the central region of the city, near Solon de Lucena Park; Attracted the interest of the government to the installation of infra-structure after the introduction of the cities and aggregations although the statal response took more time; it was responsible for the initial incursions in districts like Torre and Expedicionários that later on would become residential districts through the doings of other public institutions and, finally, consolidated the peripheral introduction of the statal housings with the construction of the State public employees city in the district of Oitizeiro. / Esta dissertação objetiva o exame da atuação construtiva da Carteira Imobiliária do Montepio do Estado da Paraíba na produção estatal de habitação na cidade de João Pessoa, de 1932 a 1963, período estabelecido entre a designação da instituição para produção de moradias em benefício do funcionalismo público até a última realização popular dela antes do exercício do BNH. Através de exaustiva pesquisa documental, realizada em acervos locais e tendo como principal fonte o Jornal A União, registro oficial das realizações do poder executivo estadual, foram recolhidos dados sobre as realizações habitacionais do instituto, possibilitando a identificação das suas vilas e conjuntos populares e posteriormente a classificação das unidades construídas e a reconstituição da planta e fachada originais. E pelo estudo da implantação das unidades populares e das casas residenciais, como eram chamadas as casas que eram construídas isoladamente e não tinham caráter popular, conseguimos determinar a relação entre o patrimônio edificado pelo Montepio e a evolução urbana de João Pessoa. E mesmo sem avassaladores planos urbanísticos ou grandes conjuntos como os de outros institutos federais, o Montepio consolidou o uso residencial na região central da cidade, próxima ao Parque Solon de Lucena; atraiu o interesse do governo para provisão da infraestrutura após a instalação das vilas e conjuntos embora as respostas estatais tenham sido comumente mais demoradas; foi responsável pelas incursões iniciais em bairros como Torre e Expedicionários que mais tarde se firmariam como bairros residenciais pela ação de outros institutos também públicos e, finalmente, consolidou a implantação periférica de habitação estatal ao conceber a Cidade do Funcionário Público Estadual no Bairro de Oitizeiro.