Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:35Z No. of bitstreams: 5 47.pdf: 2283518 bytes, checksum: 0cb380e74b0df649714290d50610a24f (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T19:01:57Z (GMT) No. of bitstreams: 5 47.pdf: 2283518 bytes, checksum: 0cb380e74b0df649714290d50610a24f (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T19:01:57Z (GMT). No. of bitstreams: 5 47.pdf: 2283518 bytes, checksum: 0cb380e74b0df649714290d50610a24f (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2012 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / (Fenologia, biologia reprodutiva, germina??o e desenvolvimento inicial de Cipocereus minensis subsp. leiocarpus N.P.Taylor & Zappi (Cactaceae) no planalto de Diamantina-MG). No Brasil a fam?lia Cactaceae ? representada por 160 esp?cies, das quais 26% ocorrem em campos rupestres. Cipocereus minensis ? uma Cactaceae end?mica dos campos rupestres da por??o mineira da cadeia do Espinha?o. Neste estudo pretendeu-se avaliar os ritmos de produ??o de flores e frutos da esp?cie, sua biologia floral e reprodutiva, al?m de conhecer seus visitantes florais e prov?veis dispersores de sementes, numa ?rea de campo rupestre do planalto de Diamantina-MG. Foram realizados testes de germina??o de sementes e avaliado o desenvolvimento inicial das plantas em diferentes substratos. A esp?cie apresentou caracter?sticas florais como antese noturna, flores brancas, volume abundante de n?ctar e estruturas do perianto r?gidas que sugeriram poliniza??o por morcegos. No entanto, observou-se tamb?m a presen?a de polinizadores diurnos como beija-flores. O sistema reprodutivo de C. minensis ? alog?mico, auto-incompat?vel e os visitantes noturnos foram mais eficientes que os diurnos na forma??o de frutos. O padr?o fenol?gico de flora??o e frutifica??o ? subanual, com picos de flora??o no in?cio e meio da esta??o seca, em abril e julho, respectivamente. Um outro pico foi observado na esta??o ?mida, em novembro, com menor produ??o de flores, por?m com maior taxa de convers?o flor/fruto. C. minensis ? uma esp?cie bem adaptada ? sazonalidade clim?tica. Os frutos apresentaram diferen?as significativas com rela??o ? ?poca de coleta quanto ao comprimento, di?metro e peso, com maiores valores na esta??o ?mida. A m?dia do n?mero de sementes por fruto foi de 958,63 ? 369,35, o peso de 1000 sementes foi de 0,53 g e a m?dia do comprimento das sementes foi de 1,5 mm. As maiores taxas de germina??o foram obtidas ap?s nove e doze meses de armazenamento (75% e 72%, respectivamente), ou em frutos coletados no est?gio mais avan?ado de matura??o (72%). No crescimento inicial as plantas apresentaram maiores di?metros e alturas no substrato preparado na propor??o 1:1:1, composto de areia, solo vermelho e esterco de boi. O armazenamento favoreceu a germina??o, que tamb?m foi alta em frutos coletados em est?gios mais avan?ados de matura??o. Substratos com melhor drenagem e aera??o favoreceram um melhor desenvolvimento inicial de plantas dessa esp?cie. Estas informa??es dar?o subs?dios para o desenvolvimento de estrat?gias de conserva??o e manejo da esp?cie. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT In Brazil the Cactaceae is represented by 160 species from which 26% occur on the rocky fields. Cipocereus minensis is an endemic Cactaceae from the rocky fields in the Minas Gerais? portion of the Espinha?o chain. In this research it was intended to evaluate the rhythms of flower and fruit production of the species, its floral and reproductive biology, and also know the floral visitors and the likely seed dispersers, in an area of the rocky fields of the Diamantina ? MG?s plateau. Seed germination tests have been performed and the initial plant growth on different substrates was analyzed. The species had floral traits such as nocturnal anthesis, white flowers, abundant volume of nectar and rigid perianth structures that suggested pollination by bats. However daily visitors as hummingbirds were also observed. The reproductive system of C. minensis is alogamic and self incompatible and the night visitors were more efficient than the daytime ones in the formation of fruits. The phenologic pattern of flowering and fruiting is sub-annual, with peak of flowering at the beginning and middle of the dry season, in april and july, respectively. Another peak was observed in the wet season in november, with lower production of flowers, but with a higher flower/fruit conversion rate . C. minensis is well adapted to the climatic seasonality. The fruits showed significant differences with respect to collection time for the length, diameter and weight, with higher measures in the wet season. The average number of seeds per fruit was 958.63 ? 369.35, the weight of 1000 seeds was 0.53 g and the mean length of the seeds was 1.5 mm. The highest germination rates were found after nine and twelve months of storage (75% and 72%, respectively) or in fruits collected with a more advanced stage of ripening (72%).In the initial growth the plants showed a higher diameter and height on the substrate prepared in the ratio 1:1:1, composed of sand, red soil and cow dung. The store promotes germination, but also in fruits collected in a more advanced stage of maturation. Substrates with better drainage and aeration favoring better initial development of this plant species. This information will provide subsidies for the development of strategies of conservation and management of the species.

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Educação, Programa de Pós-Graduação em Educação, Florianópolis, 2015. / Made available in DSpace on 2016-05-24T17:30:02Z (GMT). No. of bitstreams: 1 338113.pdf: 946750 bytes, checksum: e5ab1f9633beba8d309f80f3b3234c75 (MD5) Previous issue date: 2015 / Analisaremos, neste trabalho, duas imagens que configuram tipos distintos de mestre traçados por Nietzsche, a fim de pensarmos a questão da escola contemporânea, uma vez que está contido, nas duas ideias de mestria, o problema da cultura - logo, o da formação humana. Inicialmente apresentaremos a figura do mestre inspirado em sua juventude e que figuraria um período estético romântico de sua obra. Outra representação estratégica nos escritos de Nietzsche, que exerce importante papel ao analisarmos os tipos distintos de mestre, é o protagonista de Assim falou Zaratustra. Com base nessa obra, demonstraremos que o conceito de mestre é desconstruído, para compor outra ideia de sabedoria ou ensino. Trata-se da evocação da imagem do andarilho (Wanderer), representada pela experiência de travessia do mestre - profeta que, ao traçar caminhos diversos, rompe valores basilares, ou seja, valores morais embasados no que Nietzsche denominou "ideais ascéticos". Pois Zaratustra, absorto em reflexões, intenta cancelar tábuas avoengas, a fim de averiguar questões do conhecimento sobre a formação da cultura e criação conceitual sobre a ideia de homem.<br> / Abstract : In this work, we are going to analyse two images that sets up the different kinds of master strokes by Nietzsche, in order to think in the contemporary school, since it contained, in both ideas of mastery, the problem of the culture - before long, the human formation. Initially we are going to introduce the figure of the master inspired in his youth that would figure in a romantic aesthetic period of his work.Another strategic representation in Nietzsche written, that exercises an important role in the analyse of the diferent kinds of master, is the leading figure of "Also sprach Zarathustra". Based on this work, we are going to demonstrate that the concept of master is deconstructed to compose another idea of wisdom or teaching. It is the evocation of the image of the Wandering, represented by the experience of the master crossing that trace different paths, tear basic values, in other words, values based in what Nietzsche called "ascetic ideals". Because Zarastustra, absorbed in reflections, attempts to destroy older boards. Therefore, we are going to use the track lived by Zarastustra, in order to inquire the problem of knowledge about the formation of the man and the creation about the idea of the man.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-11-12T14:48:35Z No. of bitstreams: 1 MariaDoSocorroBezerraDaSilva_DISSERT.pdf: 8242612 bytes, checksum: 0bf088500a19fde1a0ca76c1dbc40bd5 (MD5) / Approved for entry into archive by Elisangela Moura (lilaalves@gmail.com) on 2015-11-16T14:22:56Z (GMT) No. of bitstreams: 1 MariaDoSocorroBezerraDaSilva_DISSERT.pdf: 8242612 bytes, checksum: 0bf088500a19fde1a0ca76c1dbc40bd5 (MD5) / Made available in DSpace on 2015-11-16T14:22:56Z (GMT). No. of bitstreams: 1 MariaDoSocorroBezerraDaSilva_DISSERT.pdf: 8242612 bytes, checksum: 0bf088500a19fde1a0ca76c1dbc40bd5 (MD5) Previous issue date: 2014-02-13 / A explora??o e a produ??o de hidrocarbonetos exigem metodologias muito avan?adas para se conseguir trabalhar em condi??es cada vez mais adversas. Dentro destas novas metodologias de extra??o de petr?leo surge ? inje??o de solu??es polim?ricas, que torna-se eficiente no controle da mobilidade do fluido deslocante (?gua), e sob condi??es favor?veis, implica na antecipa??o da recupera??o de ?leo e, em alguns casos, fatores de recupera??o maiores. Este m?todo consiste em adicionar pol?meros ? ?gua de inje??o para aumentar sua viscosidade, fazendo com que a ?gua se difunda mais no meio poroso e aumentando a efici?ncia de varrido no reservat?rio. Neste trabalho, estuda-se atrav?s de simula??o num?rica, a aplica??o da inje??o de solu??o polim?rica em um reservat?rio homog?neo, semissint?tico com caracter?sticas do Nordeste Brasileiro, as simula??es num?ricas foram realizadas atrav?s do simulador t?rmico STARS da CMG (Computer Modelling Group). O trabalho teve como objetivo analisar a influ?ncia de alguns par?metros de reservat?rio sobre o comportamento de produ??o do ?leo, tendo como resposta a produ??o acumulada. As simula??es da inje??o de solu??o polim?rica, al?m da simula??o da inje??o de ?gua, consistiram em diversas etapas de an?lise de sensibilidade de par?metros de reservat?rio e operacionais. Foram realizadas simula??es para analisar a influ?ncia da inje??o de ?gua, solu??o polim?rica e inje??o alternada de bancos de ?gua e de solu??o polim?rica, comparando os resultados para cada condi??o simulada. As principais vari?veis avaliadas foram: viscosidade do ?leo, porcentagem de pol?mero injetado, viscosidade do pol?mero e vaz?o de inje??o de ?gua. A avalia??o da influ?ncia das vari?veis consistiu de um planejamento experimental completo seguido de uma an?lise por Diagrama de Pareto com o objetivo de apontar quais as vari?veis seriam mais influentes sobre a resposta representada pela produ??o acumulada do ?leo. Encontrou-se que todas as vari?veis influenciaram significativamente na recupera??o de ?leo e que a inje??o de solu??o polim?rica de forma cont?nua se mostrou mais eficiente para a produ??o acumulada quando comparada a recupera??o do ?leo por inje??o cont?nua de ?gua. A recupera??o prim?ria apresentou baixos n?veis de produ??o de ?leo, a inje??o de ?gua melhora significativamente a produ??o de ?leo no reservat?rio, mas a inje??o de solu??o polim?rica surge como uma nova metodologia para o incremento da produ??o de ?leo, aumento da vida ?til do po?o e poss?vel diminui??o de ?gua produzida. / Developing an efficient methodology for oil recovery is extremely important . Within the range of enh anced oil recovery, known as EOR, the injection of polymer solutions becomes effective in controlling the mobility of displacing fluid . This method consists of adding polymers to the injection water to increase its viscosity, so that more water diffuses in to the porous medium and increasing the sweep efficiency in the reservoir. This work is studied by numerical simulation , application of the injection polymer solution in a homogeneous reservoir , semisynthetic with similar characteristics to the reservoirs of the Brazilian Northeast , numerical simulations were performed using thermal simulator STARS from CMG (Computer Modelling Group ). The study aimed to analyze the influence of some parameters on the behavior of reservoir oil production, with the response to cumulative production. Simulations were performed to analyze the influence of water injection, polymer solution and alternating injection of water banks and polymer solution, comparing the results for each simulated condition. The primary outcomes were: oil viscosity, percentage of injected polymer, polymer viscosity and flow rate of water injection. The evaluation of the influence of variables consisted of a complete experimental design followed a Pareto analysis for the purpose of pointing out which va riables would be most influential on the response represented b y the cumulative oil production . It was found that all variables significantly influenced the recovery of oil and the injection of polymer solution on an ongoing basis is more efficient for the cumulative production compared to oil recovery by continuous water injection. The primary recovery show ed low levels of oil production , water injection significantly improves the pro duction of oil in the reservoir , but the injection of polymer solution em erges as a new methodology to increase the production of oil, increasing the life of the well and possible reduction of water produced.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-15T14:35:59Z No. of bitstreams: 1 EdmirDosSantosJesus_TESE.pdf: 4442960 bytes, checksum: fde709d3dd873f5006c58ab41efad001 (MD5) / Approved for entry into archive by Elisangela Moura (lilaalves@gmail.com) on 2016-01-25T12:29:40Z (GMT) No. of bitstreams: 1 EdmirDosSantosJesus_TESE.pdf: 4442960 bytes, checksum: fde709d3dd873f5006c58ab41efad001 (MD5) / Made available in DSpace on 2016-01-25T12:29:42Z (GMT). No. of bitstreams: 1 EdmirDosSantosJesus_TESE.pdf: 4442960 bytes, checksum: fde709d3dd873f5006c58ab41efad001 (MD5) Previous issue date: 2014-02-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / O objetivo desta pesquisa foi estimar avaliar a evapotranspira??o na microrregi?o do Serid? a partir de dados pontuais de alguns munic?pios pertencentes para verificar os potenciais evapotranspirom?tricos da regi?o. Os resultados apresentados pelos principais m?todos de estimativa deram conta de que o m?todo de Hagreaves-Samani foi o que apresentou melhor ajuste em rela??o ao m?todo de Penman-Monteith (FAO 56), mostrando ser vi?vel de utiliza??o, apresentando ?muito bom? desempenho conforme os dados medidos e estimados para as localidades estudadas. Os m?todos de Penman, Thornthwaite e Romanenko n?o responderam muito bem em pelo menos 4 das localidades estudadas, ficando com o ?p?ssimo? desempenho para o estudo devido a an?lise estat?stica ter mostrado resultados menos significativos e n?o significativos em rela??o ao m?todo padr?o de Penman-Monteith. A simula??o de cen?rios segundo o IPCC foram plotados conforme os aumentos na temperatura m?dia do ar, e utilizando dos m?todos de evapotranspira??o mostraram que os valores resultantes apontam alta na metade oeste da regi?o do Serid?. E que devido a essas previs?es de aumento gradativo na temperatura e conseq?entemente na evapotranspira??o, os munic?pios pertencentes a essa regi?o poder?o ser castigados com o alto valor no d?ficit h?drico. Em complemento a esses resultados foram levantadas quest?es com rela??o ao cont?nuo desenvolvimento da regi?o, pois com o aumento nos altos valores de Eto poder? acarretar s?rios problemas ?s comunidades que necessitam da ?gua dispon?vel em a?udes e reservat?rios destinados ao abastecimento para consumo, agricultura familiar e a agropecu?ria.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-10T20:59:53Z No. of bitstreams: 1 GleidsonLimaPinheiro_DISSERT.pdf: 2590622 bytes, checksum: 06de3b4091ef1d22ceaba6df4aead4d0 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-05-12T19:33:54Z (GMT) No. of bitstreams: 1 GleidsonLimaPinheiro_DISSERT.pdf: 2590622 bytes, checksum: 06de3b4091ef1d22ceaba6df4aead4d0 (MD5) / Made available in DSpace on 2016-05-12T19:33:54Z (GMT). No. of bitstreams: 1 GleidsonLimaPinheiro_DISSERT.pdf: 2590622 bytes, checksum: 06de3b4091ef1d22ceaba6df4aead4d0 (MD5) Previous issue date: 2014-08-15 / Oleodutos sofrem corros?o do tipo qu?mica, devido a tens?o, entre outros mecanismos, e eletroqu?mica devido a grande quantidade de salmoura (?gua e cloretos), presentes nos fluidos produzidos na forma??o. Este trabalho tem como objetivo estudar a efici?ncia de um inibidor de corros?o comercial, usado na ind?stria do petr?leo, na inibi??o de corros?o agindo isoladamente e em mistura com microemuls?o (ME) na propor??o [1:1]. A ME foi obtida a partir dos seguintes constituintes: ?gua de torneira (fase aquosa), querosene (fase oleosa), n-butanol (cotensoativo) e UNT L90 (tensoativo). O inibidor possui mecanismo de prote??o tipo f?lmico, na prote??o do a?o carbono API 5L A210, muito usado na constru??o de oleodutos terrestres. Os experimentos foram formulados em solu??es salinas com 3% de NaCl que funcionam como meio agressivo, na presen?a e aus?ncia de borbulhamento do g?s (CO2), em temperatura ambiente (25?C). O inibidor estudado apresenta em sua fase ativa compostos de am?nio quatern?rio e derivados de imidazolina, e foi caracterizado como um inibidor formador de filme. Os resultados de efici?ncia de inibi??o foram avaliados por dados experimentais de densidades de corrente de corros?o atrav?s da t?cnica eletroqu?mica polariza??o linear, com extrapola??o de Tafel. O inibidor comercial agindo isoladamente mostrou-se mais eficiente no combate ? corros?o do que em microemuls?o quando n?o houve borbulhamento de CO2. Quando ocorreu a satura??o da solu??o de trabalho com CO2, observou-se uma eleva??o da inibi??o ao misturar-se o aditivo comercial com a microemuls?o. Tamb?m se constatou que o excesso de CO2 dissolvido na solu??o de trabalho implicou em uma eleva??o da taxa de corros?o, comparado aos ensaios an?logos realizados na aus?ncia do borbulhamento cont?nuo de CO2. Os ensaios foram realizados em concentra??es de inibidores de 10, 20, 40, 100, 200 e 400 ppm. As melhores efici?ncias foram observadas nos ensaios com concentra??o de 40 ppm, na aus?ncia do borbulhamento, e 200 ppm, quando o ensaio foi realizado na presen?a de borbulhamento com CO2. Os dados experimentais se ajustaram ao modelo de Langmuir, sugerindo a forma??o de um filme de monocamadas. / Pipelines suffer from chemical, under tension, and other kinds of corrosion mechanisms, as well as electrochemica l corrosion caused by the existing large amount of brine (water and chlorides) in the produced fluids. The goal of this work is to research the efficiency of a commercial corrosion inhibitor, used in pipelines on the oil industry, on the corrosion inhibition of the API 5L A210 iron, which is widely used on the overland pipelines constructions, acting alone and in a mixture with micro emulsion (ME) on a [1:1] ratio. The ME was obtained from the following constituents: tap water (aqueous phase), kerosene (oily phase), n-butanol (co-surfactant), and UNT L90 (surfactant). We observed the formation of protective film on the steel surface as an inhibitor?s protection mechanism. The experiments were formulated in saline solutions with 3% of NaCl that functioned as aggressive environment, on the presence and absence of gas bubbling (CO2), in room temperature (25?C). The studied inhibitor exhibits in its active phase quaternary ammonium compounds and imidazoline derivatives, which means it is a film-forming inhibitor. The inhibition efficiency results are evaluated by experimental data of corrosion current density by the electrochemical technique of linear polarization with Talef extrapolation. The inhibitor exhibit two distinct behaviors: in medium of saturation with CO2 we observed an increase of inhibition by blending the commercial additive with the micro-emulsion, and in medium without saturation of CO2 we observed a higher efficiency when the inhibitor was acting singly. We also could find that the excess of dissolved CO2 in the working solution resulted in an increase of the corrosion rate, compared to similar experiments in the absence of continuous bubbling of CO2. The best efficiencies were observed in the experiments with concentration of 40ppm without bubbling, and 200ppm when the experiments were performed in the presence of bubbling with CO2. The experimental data fitted to the Langmuir model, suggesting the formation of a film made of monolayers.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-04-02T16:35:08Z No. of bitstreams: 1 AlbanoRochaDaCosta_DISSERT.pdf: 6363234 bytes, checksum: 2eccacf967f070fdd15308af643337d4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-04-09T22:53:58Z (GMT) No. of bitstreams: 1 AlbanoRochaDaCosta_DISSERT.pdf: 6363234 bytes, checksum: 2eccacf967f070fdd15308af643337d4 (MD5) / Made available in DSpace on 2018-04-09T22:53:58Z (GMT). No. of bitstreams: 1 AlbanoRochaDaCosta_DISSERT.pdf: 6363234 bytes, checksum: 2eccacf967f070fdd15308af643337d4 (MD5) Previous issue date: 2018-02-16 / Foram 12 anos de trabalhos com Tecnologia, Educa??o Empreendedora, Startups e Inova??o, que permitiram a viv?ncia e comprova??o da efic?cia de pr?ticas, metodologias e ferramentas utilizadas na condu??o de projetos das mais variadas ?reas, escopos e objetivos. Nesse ambiente, foi poss?vel identificar que o maior esfor?o de um projeto est? no seu in?cio e que ? significante o n?mero de pessoas que ainda erram na escolha ou na aplica??o de m?todos e ferramentas, seja para iniciar ou para inovar em seus projetos. Tais constata??es, aliadas ao objetivo de automatizar os processos internos das empreitadas, resultaram no desenvolvimento de um framework funcional para dar o pontap? inicial em projetos de forma pr?tica e ?gil, buscando reduzir os riscos, por?m, ao mesmo tempo, mirando a inova??o. Nasce, assim, a Estilinguy, uma empresa criada para ajudar pessoas a realizarem os seus sonhos. Desenvolvendo e aplicando m?todos e ferramentas de inova??o para que, de forma simples e objetiva, qualquer pessoa possa idealizar, planejar e executar suas ideias e seus projetos mirabolantes. / It was 12 years of working with Technology, Entrepreneurial Education, Startups and Innovation, that allowed the experience and evidence of the effectiveness of practices, methodologies and tools for the conduction of projects from the most varied areas, scopes and objectives. In this environment, it was identified that the greatest effort of a project is at its beggining and that the number of people that still make mistakes in the choice or application of methods and tools is very significant, either to start or to innovate in their projects. All these findings, allied to the goal of automating these internal processes, resulted in the developement of a practical framework to kick-start projects in a practical and agile way, trying to reduce risks, but at the same time, aiming at innovations. This is how Estilinguy was born, a company created to help people make their dreams come true. By developing and applying innovative methods and tools so that, in an easy and objective way, anyone may idealize, plan and execute their crazy ideas and projects.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-23T18:06:12Z No. of bitstreams: 1 MariaFrancineteDamasceno_TESE.pdf: 6648933 bytes, checksum: 865fa3a7d40f91ddc21e393b3a751df2 (MD5) / Approved for entry into archive by Monica Paiva (monicalpaiva@hotmail.com) on 2017-02-23T18:16:54Z (GMT) No. of bitstreams: 1 MariaFrancineteDamasceno_TESE.pdf: 6648933 bytes, checksum: 865fa3a7d40f91ddc21e393b3a751df2 (MD5) / Made available in DSpace on 2017-02-23T18:16:54Z (GMT). No. of bitstreams: 1 MariaFrancineteDamasceno_TESE.pdf: 6648933 bytes, checksum: 865fa3a7d40f91ddc21e393b3a751df2 (MD5) Previous issue date: 2014-11-14 / Nesta pesquisa, temos como objeto de estudo o fazer da Educa??o a Dist?ncia (EaD) em sua implica??o com a a??o docente presencial. Partimos do pressuposto de que os sentidos atribu?dos pelos formandos nessa modalidade relacionam-se a um fazer da EaD que implica em mudan?as na sua a??o como docente. Temos, assim, o objetivo de compreender os sentidos atribu?dos ? EaD por professores que s?o alunos - em segunda forma??o e nesta modalidade - e a implica??o desta forma??o, em processo, com a sua a??o docente. Delimitamos a pesquisa para o estudo dos sentidos atribu?dos pelos formandos do Polo de Apoio Presencial da Universidade Aberta do Brasil (UAB) de Floriano (PI), cujos cursos s?o ofertados pela Universidade Federal do Piau? (UFPI) por meio da pol?tica p?blica do sistema UAB. Consideramos importante estudar o fazer e as implica??es de uma modalidade de educa??o, impulsionada e amplamente adotada atrav?s de uma pol?tica p?blica nacional para atender n?o exclusivamente, mas prioritariamente, a forma??o de professores, o que pode influenciar na qualidade da educa??o no Brasil. Para tanto, utilizamos como unidade principal de refer?ncia entrevistas realizadas e interpretadas por meio da metodologia da Entrevista Compreensiva. Desvelamos, a partir da falas, que embora o fazer da EaD tenha pontos que mere?am ser revistos, seu processo formativo implica em mudan?a positiva na a??o do docente. Com este estudo, visamos contribuir para as pesquisas sobre a EaD e o uso das TICs na educa??o, o que pode apontar caminhos novos para o processo reflexivo e decis?rio no que concerne ao fazer da EaD e aos processos de forma??o docente. / Nesta pesquisa, temos como objeto de estudo o fazer da Educa??o a Dist?ncia (EaD) em sua implica??o com a a??o docente presencial. Partimos do pressuposto de que os sentidos atribu?dos pelos formandos nessa modalidade relacionam-se a um fazer da EaD que implica em mudan?as na sua a??o como docente. Temos, assim, o objetivo de compreender os sentidos atribu?dos ? EaD por professores que s?o alunos - em segunda forma??o e nesta modalidade - e a implica??o desta forma??o, em processo, com a sua a??o docente. Delimitamos a pesquisa para o estudo dos sentidos atribu?dos pelos formandos do Polo de Apoio Presencial da Universidade Aberta do Brasil (UAB) de Floriano (PI), cujos cursos s?o ofertados pela Universidade Federal do Piau? (UFPI) por meio da pol?tica p?blica do sistema UAB. Consideramos importante estudar o fazer e as implica??es de uma modalidade de educa??o, impulsionada e amplamente adotada atrav?s de uma pol?tica p?blica nacional para atender n?o exclusivamente, mas prioritariamente, a forma??o de professores, o que pode influenciar na qualidade da educa??o no Brasil. Para tanto, utilizamos como unidade principal de refer?ncia entrevistas realizadas e interpretadas por meio da metodologia da Entrevista Compreensiva. Desvelamos, a partir da falas, que embora o fazer da EaD tenha pontos que mere?am ser revistos, seu processo formativo implica em mudan?a positiva na a??o do docente. Com este estudo, visamos contribuir para as pesquisas sobre a EaD e o uso das TICs na educa??o, o que pode apontar caminhos novos para o processo reflexivo e decis?rio no que concerne ao fazer da EaD e aos processos de forma??o docente.

Pas de résumé en français / One of the hallmarks of cancer cells is to exhibit increased uptake and consumption of glucose.3-5% of the glucose entering into the cell leads to a minor pathway of the glucose metabolismknown as the hexosamine biosynthetic pathway (HBP). UDP-N-acetylglucosamine is the endproduct of HBP and is used as substrate by OGT (O-GlcNAc transferase) to modify diverserange of nuclear and cytoplasmic proteins with a recently characterized post-translationalmodification called O-GlcNAcylation. It corresponds to the addition of sugar moiety O-linked β-N-acetylglucosamine (O-GlcNAc) on serine or threonine residue of proteins. This process isantagonized by another enzyme called O-GlcNAcase (OGA). Recent studies indicated thepresence of increased O-GlcNAcylation level in several cancer cells. Moreover, inhibition ofOGT has been shown to reduce in vivo and in vitro tumor growth of breast cancer cells.However, the relationship between O-GlcNAcylation and the response to anti-cancer therapy hasnot been studied. Tamoxifen is the oldest and most prescribed selective-estrogen receptormodulator (SERM) for patients with estrogen receptor (ER)-positive breast cancer. Tamoxifen isknown to reduce tumor growth and invasion. Despite its beneficial effects de novo and acquiredresistance are great obstacles in its clinical effectiveness. We found that O-GlcNAc elevation inMCF-7 cells protected them from tamoxifen-induced cell death. Increased O-GlcNAc alsoincreased PI3-K/Akt signaling. However, the protective effect of PUGNAc+glucosamine fromtamoxifen-induced cell death is independent of PI3K/Akt pathway. Increased O-GlcNAcylationalso led to reduced ESR1 promoter activity and decreased expression of ERα at mRNA andprotein levels. The decrease in ERα expression is correlated with a reduced expression of twotamoxifen regulated genes i.e. early growth response 1 and p21 Waf1/Cip1. In conclusion, thisstudy showed for the first time the involvement of O-GlcNAcylation in reducing tamoxifen142sensitivity in MCF-7 cells. Thus, OGT can act as a novel therapeutic target for treatment oftamoxifen resistant cells.

Les démences séniles sont caractérisées, au niveau moléculaire, par l’agrégation de quelquesprotéines-clés. On peut donner comme exemple l’α-synucléine, dans la maladie de Parkinson,ou le peptide β-amyloïde et la protéine Tau, dans la maladie d’Alzheimer. Le mauvaisrepliement de ces protéines est souvent évoqué, même si elles n’adoptent pas une structurestable lorsqu’elles sont isolées en solution. La protéine Tau est une protéine neuronaleappartenant à la famille des protéines MAP (protéines associées aux microtubules). Saprincipale fonction est de stimuler la polymérisation de la tubuline en microtubules, assurantainsi le transport cytoplasmique au sein d’une cellule. Tau ainsi que des mutants de Tau ont étéimpliqués dans de nombreuses pathologies neurodégénératives regroupées sous le terme detauopathies, dont la plus connue est la maladie d’Alzheimer. Bien qu’aucune mutation de Taun’ait été décrite dans la maladie d’Alzheimer, Tau joue un rôle-clé dans cette pathologie. Elleest caractérisée par la présence d’agrégats fibrillaires de protéines Tau hyperphosphoryléesnommés PHF (Paired Helical Filaments) qui envahissent progressivement le cerveau. Cesobservations sont associées à un déséquilibre entre phosphorylation et déphosphorylation.Malgré les différentes études menées sur le sujet, le mécanisme d’agrégation n’est pas compriset plusieurs pistes sont envisagées, notamment l’implication des modifications posttraductionnelles.Nous avons déterminé le profil de modifications de la protéine Tau parspectroscopie RMN et étudier leur implication dans le processus d’agrégation parspectrophotométrie. Parmi les modifications covalentes existantes, nous avons étudiél’acétylation des résidus lysine et l'O-β-N-acétylglucosaminylation des résidus sérine etthréonine. La troisième est une modification conformationnelle qui consiste à stimulerl’échange conformationnel des prolines par des enzymes à activité peptidyl prolyl cis-transisomérase (PPIase). / Senile dementia are characterized by protein aggregation such as α-synuclein in Parkinsondisease or β-amyloid peptide and Tau protein in Alzheimer disease. Protein misfolding has beenevoked in the pathological processes even though these proteins do not adopt a stable threedimensionalstructure in solution. Tau protein belongs to the MAP (Microtubule-AssociatedProtein) family. It stimulates tubulin polymerization into microtubule allowing for cytoplasmictransport in cell. Tau and its mutated forms are found in neurodegenerative diseases,collectively referred to as tauopathy, the most famous being Alzheimer’s disease. Thesepathologies are characterized by fibrillary aggregates of hyperphosphorylated Tau namedPaired Helical Filaments (PHF) that invades gradually throughout the brain. These observationsare correlated with imbalance between phosphorylation and dephosphorylation in AD brains.Despites extensive studies, the aggregation mechanism is still not understood and severalpathways were considered, especially posttranslational modifications other thanphosphorylation. We have investigated the modifications of Tau protein by NMR spectroscopyand studied effect of these modifications on aggregation process by spectrophotometry. Amongcovalent modifications, we have studied lysine acetylation and Ser/Thr O-β-Nacétylglucosaminylation.The third investigated modification is the conformational switch ofproline residues catalyzed by a peptidyl prolyl cis trans isomerase.

Le glioblastome multiforme (GBM) est la tumeur cérébrale maligne la plus fréquente et la plus agressive chez l’adulte. Malgré l’utilisation concomitante de la chirurgie avec la radiothérapie et la chimiothérapie (protocole de Stupp), le pronostic des patients reste sombre avec un taux de survie à 5 ans inférieur à 10 %. La présence de cellules souches cancéreuses (CSC) favorise le maintien de la tumeur puis l’échappement tumoral responsable de la rechute des patients. Il est donc nécessaire d’identifier de nouvelles cibles thérapeutiques pour améliorer la prise en charge de ces patients. Dans ce contexte, l’immunothérapie, stratégie utilisant le système immunitaire pour traiter le cancer, représente une voie prometteuse.Nous identifions ici le OAcGD2 comme un nouvel antigène tumoral du GBM et a partir de biopsie tumorale provenant d’une cohorte de 37 patients. L’utilisation de primoculture de GBM nous permet de plus de démontrer que ce marqueur est également exprimé par les cellules souches de GBM. Nous apportons la preuve de concept de l’immunothérapie du GBM à l’aide d’anticorps monoclonaux anti-OAcGD2 in vitro et in vivo. A côté de la cytotoxicté immunologique, nous identifions un nouveau mécanisme de mort programmé, l’oncose, mis en jeu par les AcM anti-OAcGD2 permettant de sensibiliser ces cellules aux agents de chimiothérapie comme le témolozomide. L’utilisation de l’immunothérapie ciblant le marqueur gangliosidique tumoral OAcGD2 pourrait ainsi permettre d’améliorer l’efficacité de la thérapeutique actuelle (protocole de Stupp) du GBM / Glioblastoma multiforme (GBM) is the most common and agressive primary brain tumors in adults. Despite the concomitant use of surgery with radiotherapy and chemotherapy, the prognosis of patients remains extremely low. The presence of cancer stem cells (CSC) promotes the maintenance of the tumor and then the tumor escape responsible for the relapse of the patients. Therefore it is necessary to identify new therapeutic targets to improve the management of these patients. In the past few decades, immunotherapy represents an important part of treating certain type of cancer. It uses the immune system to treat cancer. Here, we found that O-acetyl GD2 (OAcGD2) is expressed in surgically resected human glioblastoma tissue. In addition, we demonstrated that 8B6 monoclonal antibody specific for OAcGD2 could effectively inhibit glioblastoma cell proliferation in vitro and in vivo. Mostly, we found that OAcGD2 was expressed on the GBM stem cells. We also observed that mAb 8B6 promoted the elimination of GBM via a oncosis-like mechanism. Moreover, this mechanism of programmed cell death induced by anti-OAcGD2 mAbs, sensitizes GBM cells and CSCs to chemotherapy agents such as temolozomide (TMZ). Taken together, these results indicate that O-acetylated GD2 represents a novel antigen for immunotherapeutic-based treatment of high-grade gliomas, and that anti-OAcGD2 mAbs combined with TMZ could enhance therapeutic response in GBM.