This thesis aims to develop the basis of market analysis and marketing strategy recommendations for the company MK.LD which provides services to clients in the area of Supply chain management. This strategy should also help to get more clients and retain existing ones and increase the company's competitive advantage in the market.

This Master‘s Thesis is focused on the analysis of the company Kovolit. It is a joint-stock company, which is engaged in industrial production - smithery, foundry. The aim of my thesis is to analyze a company and propose appropriate solutions to improve the situation. Among the analysis include: internal and external environment, Porter's model, fundamental and financial analysis. Lastly, the SWOT analysis. Before practical part is the theoretical section, witch includes the characteristics of these analysis. The outcomes are proposals to improve the efficiency, effectiveness and financial situation.

Made available in DSpace on 2015-04-14T13:46:57Z (GMT). No. of bitstreams: 1 416790.pdf: 6091320 bytes, checksum: 55225ee92f3918e469afad55ec52ed2e (MD5) Previous issue date: 2009-03-30 / Na presente tese foi analisada a postura do governo municipal do Rio Grande e do Estado frente ?s incisivas cr?ticas do jornal Echo do Sul ao descaso dos poderes p?blicos para com a necessidade de implanta??o das pol?ticas de higiene e sa?de, em meio ? epidemia de peste bub?nica que assolou a cidade entre os anos de 1903-1904. Notou-se que o processo de higieniza??o da cidade do Rio Grande, que por for?a de leis e pela necessidade diante da crise, somente se desenvolveu em fins de 1905. Essa iniciativa das autoridades p?blicas, muito incentivada pelo jornal Echo do Sul, n?o apenas foi um instrumento para combater as epidemias, mas principalmente, uma for?a remodeladora do modo de vida da popula??o rio-grandina. Portanto, buscou-se nessa tem?tica compreender as posturas do governo e da imprensa frente ? crise end?mica, como tamb?m a transforma??o social e cultural da popula??o rio-grandina.

Submitted by Irvana Coutinho (irvana@ufpa.br) on 2013-09-06T15:07:41Z No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_VamosVacinaDoencas.pdf: 1757250 bytes, checksum: aaf98b07bc0c0f63a1c0207a8ca2ed0c (MD5) / Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2013-09-16T17:13:41Z (GMT) No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_VamosVacinaDoencas.pdf: 1757250 bytes, checksum: aaf98b07bc0c0f63a1c0207a8ca2ed0c (MD5) / Made available in DSpace on 2013-09-16T17:13:41Z (GMT). No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_VamosVacinaDoencas.pdf: 1757250 bytes, checksum: aaf98b07bc0c0f63a1c0207a8ca2ed0c (MD5) Previous issue date: 2006 / CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico / Desde o final do século XIX e, até, o início do século XX, Belém na fala do intendente Antonio Lemos era conhecida como a “necrópole” paraense. Doenças e epidemias estavam no centro do debate das práticas médico-sanitárias. O higienismo de médicos tornou se discurso recorrente de intervenção no espaço cotidiano dos moradores, onde as campanhas de profilaxias foram alçadas enquanto responsáveis pela cura da cidade. As ações propostas por esculápios cientistas geraram tensões entre moradores e autoridades públicas diante a aliança do saber médico e o poder público, sobre a qual me propus analisar para explicar o dia-a-dia das medidas coercitivas, no intuito de entender essa aliança. Analisando artigos na imprensa, literatos, jornalistas, políticos, relatos médicos, mensagens de governo, relatórios, fotografias e charges foi possível acompanhar os significados atribuídos pelos contemporâneos em relação as epidemias da varíola, tuberculose e febre amarela, por exemplo, por parte dos saberes médico-sanitários. A belle époque em Belém deixou de ser nessa dissertação um cristal historiográfico, diante as adversidade do viver de sujeitos anônimos. Belém tornou-se um laboratório de experiências, os médicos propunham curá-la para alcançar o tão propalado desenvolvimento econômico ou progresso. A consolidação dessa aliança coube à responsabilidade do renomado sanitarista Oswaldo Cruz, que desembarcou, em 1910, na capital paraense para combater a febre amarela, com carta branca do governador João Coelho. Por outro lado, a cura da cidade ou “necrópole” paraense teve significados mais amplos, destacando-se o sepultamento do mal amarílico, como também, concomitantemente, o sepultamento da oligarquia do coronel Antonio Lemos. / Since the end of the nineteenth century until the beginning of the twentieth century, Belém, according to the intendant Antonio Lemos, was known as the “paraense necropolis”. Illnesses and epidemics have been in the focus of the discussion of medical-sanitary practices. The doctor’s hygienism has become recurrent speech of intervention in the inhabitants’ daily Space, where the prophylaxes’ campaigns were considered responsible for the cure of the city. The proposed actions by medical doctors create tensions among inhabitants and public Authorities in face of the alliance between the medical knowledge and the government, topic Which I have chosen to analyze in order to explain the day-to-day of the coercive actions, with The intention of understand such alliance. Analyzing medical articles in the press, literary theoretical, journalists, politicians, Medical reports, government’s messages, photographs and charges, it was possible to follow The meanings attributed by the contemporaries related to epidemics as the smallpox, Tuberculosis and yellow fever, for example, considering the medical knowledge. The Belle Époque in Belém it was no more considered in this paper as a historiographical crystal, in face Of the adversity of anonymous citizens’ way of life. The city has become a laboratory of Experiences that propose to cure it in order to reach such divulged economic development. The city has been left in the doctor’s hands. The consolidation of this alliance has been given To the well-known sanitarian Oswaldo Cruz, that arrived in Belém City in 1910 to fight Against the yellow fever with the complete support of the governor João Coelho. On the other Hand, the cure of the city or “paraense necropolis” has brought out larger significances, Among them, coincidentally, the burial of the amarílico evil, as well as, the burial of colonel Antonio Lemos’ oligarchy.

El trabajo aquí presentado busca, por tanto, comprender el cómo fue usada, socialmente usada, la institución municipal de una villa mediana, tanto desde el punto de vista interno como desde su proyección al exterior. Para hacerlo hemos elegido los años que corrieron como bisagra entre los siglos XVI y XVII y un territorio concreto: la villa de Palomares del Campo en el partido de Huete (Cuenca). El caso de Palomares encaja bien en el sistema que nos permite plantear los supuestos sobre la significación centralizadora de la Monarquía en estos años. Para comprender el marco local es fundamental tener en cuenta las relaciones entre el concejo y la Monarquía y los efectos que los designios de ésta pudieron tener sobre aquél. / The work here presented search, therefore, since there was used, socially secondhand the municipal institution of a medium villa, both from the internal point of view and from the project to do it we have chosen the years that ran as hinge between the XVIth and XVIIth century and a concrete territory the villa of Dovecots of the field in Huete's (Cuenca) .El case of Dovecots fits well in the system that allows us to raise the suppositions on the meaning centralizadora of the Monarchy in these years. To understand the local frame is fundamental bear the relations in mind betweenthe council and the Monarchy and the effects that the plans of this one could have on that one

Peste des petits ruminants virus (PPRV) belongs to the family paramyxoviridae which comprises non segmented negative sense RNA viruses including measles and rinderpest virus. PPRV is the causative agent of peste des petits rumaninats disease (also known as sheep or goat plague disease) in small ruminants. The viral genome contains a non segmented negative sense RNA encapsidated by viral encoded nucleocapsid protein (N-RNA). Viral transcription is carried out by the virus encoded RNA dependent RNA polymerase complex represented by the large protein L and phosphoprotein P. Viral transcription begins at the 3’ end of the genome synthesising all the viral transcripts (3’-N-P-M-F-HN-L-5’). A remarkable feature common to all members of Paramyxoviridae family is the gradient of transcription from 3’ end to the 5’ end due to attenuation of polymerase transcription at each gene junction. The objectives of the present study are characterization of peste des petits ruminants virus transcription and the associated activities required for post transcriptional modification of viral mRNA. In addition, an attempt has been made to develop in vitro transcription with heterologous combination of PPRV and RPV polymerase proteins. The first reaction in capping involves removal of γ-phosphate from triphosphate ended precursor mRNA by RNA triphosphatase. The domain having RNA triphosphatase activity within the L protein has been identified and expressed independently in E. coli. The details of the objectives are presented below. 1. Development of in vitro transcription system for PPRV mRNA synthesis In order to develop an in vitro transcription reconstitution system for PPRV, the viral RNP complex comprising large (L), phospho (P) and N protein encapsidating viral genomic RNA was purified from virus infected Vero cells. The in vitro transcription reconstituted system with RNP complex was able to synthesise all the viral mRNA as analysed by RT-PCR. As a control, total RNA from virus infected cells was isolated and analysed by RT-PCR. In order to refine the in vitro transcription system, separately expressed recombinant polymerase complex was used to reconstitute transcriptional activity in vitro. For this,viral genomic RNA (N-RNA) was purified from PPRV infected cells using CsCl density gradient centrifugation. The recombinant baculovirus for PPRV P protein was earlier generated in the lab. A recombinant baculovirus harbouring the L gene of PPRV was generated in the present study (described in part one). The viral RNA polymerase consisting of L-P complex was expressed in Sf21 insect cells and partially purified by ultra centrifugation on 5-20% glycerol gradient. Glycerol gradient fraction containing the L-P complex was found to be active in the in vitro transcription reconstitution system. Further quantitation of transcripts made in vitro and in infected cells has been carried out by real time PCR. Notably, the gradient of polarity of transcription of viral mRNA observed in vitro with the partially purified recombinant L-P complex was similar to the gradient observed in infected cells. Host proteins have been shown to modulate the transcription of many paramyxoviruses. In order to test the role of host factors, uninfected cell lysate of Vero cells was added to the in vitro transcription reaction and the transcript level was measured by real time PCR. The result showed an increase in the transcription by addition of host proteins suggesting the involvement of host factors in viral transcription. Further, the newly developed in vitro reconstitution system was used to test if recombinant L and P proteins of RPV can functionally replace PPRV L and P protein in the in vitro transcription complementation assay. The result presented in part one indicates that the L or P protein of PPRV can be replaced by RPV L and P protein in heterologous transcription reconstitution system ,with a reduced efficiency. However, the homologous polymerase complex of RPV failed to recognise the N-RNA genomic template of PPRV. 2. RNA triphosphatase activity of PPRV L protein and identification of RNA triphosphatase domain Post transcriptional modification of mRNA such as capping and methylation determines the translatability of viral mRNA by cellular ribosome. In negative sense RNA viruses, synthesis of viral mRNA is carried out by the viral encoded RNA polymerase in the host cell cytoplasm. Since the host capping and methylation machinery is localized to the nucleus, viruses should either encode their own mRNA modification enzymes or adopt alternative methods as has been reported for orthomyxoviruses (cap snatching) and picornaviruses (presence of IRES element). In order to test, if PPRV RNA polymerase possesses any of the capping activities, the RNP complex containing the viral N-RNA and RNA polymerase (L-P) were purified from virion. Using the purified RNP complex, the first activity required for mRNA capping, RNA triphosphatase was tested and the results are described in part two. RNP complex purified from virion showed both RNA triphosphatase (RTPase) activity. The RNA triphosphatase from viruses, fungi and other eukaryotes have been classified into two groups, metal dependent and metal independent. The cleavage of the γ-phosphate from triphosphate ended precursor mRNA by L protein of PPRV was found to be metal dependent. So, by the metal dependency of the RTPase reaction, PPRV L protein was assigned to the metal dependent RTPase tunnel family. One of the key features of metal dependent RTPase group members is the ability to hydrolyse γ-β phosphoanhydride bond of NTPs. PPRV L protein associated with RNP complex also was also able to cleave γ-β phosphoanhydride bond of NTPs. Owing to the large size of L protein (240 KDa), it is conceivable that the L protein functions in a modular fashion for different activities pertaining to mRNA synthesis and post transcriptional modification. Sequence comparison of L proteins from different morbilliviruses revealed the presence of three conserved domains namely domain I (aa 1-606), domain II (aa 650-1694) and domain III (aa 1717-2183). Domain II has the catalytic motif for viral RNA dependent RNA polymerase. Multiple sequence alignment of PPRV L protein with known RNA triphosphatases predicted a two hundred amino acid long region on L protein comprising the C terminus of domain II and N terminus of DIII as a possible candidate for RNA triphosphatase domain. The above predicted domain was cloned and expressed in E. coli. The ability of the purified recombinant RTPase domain to cleave γ-β phosphoanhydride bond of RNA was tested. The results described in part two suggest that the predicted RTPase domain has RNA triphosphatase activity. In addition to RNA triphosphatase, the RTPase domain also has the NTPase activity. The RNA triphosphatase of DNA viruses, yeasts and other fungi have three motifs essential for enzyme activity. Motif A and motif C are rich in glutamate and are involved in metal binding. Motif B is rich in basic amino acids and forms the centre for catalysis. The glutamate residue (E1647) of motif A of PPRV L protein RTPase domain was converted to alanine and the loss of RTPase activity was assessed. The results summarised in appendix 1 shows that the E1647A mutant has reduced RNA triphosphatase and NTPase activity.

Les maladies émergentes à transmission vectorielle sont une préoccupation croissante et particulièrement lorsqu’elles affectent les chevaux, une population spécifiquement à risque vis-à-vis de la propagation de maladies. En effet, les chevaux voyagent fréquemment et, malgré l’impact sanitaire et économique des maladies équines, les règlementations sanitaires et les principes de biosécurité et de traçabilité censés assurer la sécurité des mouvements d'équidés ne sont pas toujours en place. Notre travail propose d'améliorer la surveillance des maladies à transmission vectorielle chez les chevaux en utilisant différentes méthodes pour estimer la probabilité d'émergence d'une maladie. Tout d'abord, nous avons développé un modèle quantitatif et spatio-temporel combinant différentes probabilités pour estimer les risques d'introduction de la peste équine et de l’encéphalose équine. Ces combinaisons permettent d’obtenir une image plus détaillée du risque posé par ces agents pathogènes. Nous avons ensuite évalué des systèmes de surveillance syndromique par deux approches méthodologiques: l'approche classique avec un seuil d'alarme basé sur un multiple de l'erreur standard de prédiction, et l'approche bayésienne basée sur le rapport de vraisemblance. Nous avons travaillé ici principalement sur la détection précoce du virus West Nile en utilisant les symptômes nerveux des chevaux. Les deux approches ont fourni des résultats prometteurs, mais l’approche bayésienne était particulièrement intéressante pour obtenir un résultat quantitatif et pour combiner différentes informations épidémiologiques. Pour finir, l'approche bayésienne a été utilisée pour combiner quantitativement différentes sources d'estimation du risque : surveillance syndromique multivariée, et combinaison de la surveillance syndromique avec les résultats d’analyses de risques. Ces combinaisons ont données des résultats prometteurs. Ce travail, basé sur des estimations de risque, contribue à améliorer la surveillance des maladies à transmission vectorielle chez les chevaux et facilite la prise de décision. Les principales perspectives de ce travail sont d'améliorer la collecte et le partage de données, de mettre en oeuvre une évaluation complète des performances des systèmes de surveillance multivariés, et de favoriser l'adoption de ce genre d’approche par les décideurs en utilisant une interface conviviale et en mettant en place un transfert de connaissance. / Emerging vector-borne diseases are a growing concern, especially for horse populations, which are at particular risk for disease spread. In general, horses travel widely and frequently and, despite the health and economic impacts of equine diseases, effective health regulations and biosecurity systems to ensure safe equine movements are not always in place. The present work proposes to improve the surveillance of vector-borne diseases in horses through the use of different approaches that assess the probability of occurrence of a newly introduced epidemic. First, we developed a spatiotemporal quantitative model which combined various probabilities in order to estimate the risk of introduction of African horse sickness and equine encephalosis. Such combinations of risk provided more a detailed picture of the true risk posed by these pathogens. Second, we assessed syndromic surveillance systems using two approaches: a classical approach with the alarm threshold based on the standard error of prediction, and a Bayesian approach based on a likelihood ratio. We focused particularly on the early detection of West Nile virus using reports of nervous symptoms in horses. Both approaches provided interesting results but Bayes’ rule was especially useful as it provided a quantitative output and was able to combine different epidemiological information. Finally, a Bayesian approach was also used to quantitatively combine various sources of risk estimation in a multivariate syndromic surveillance system, as well as a combination of quantitative risk assessment with syndromic surveillance (applied to West Nile virus and equine encephalosis, respectively). Combining evidence provided promising results. This work, based on risk estimations, strengthens the surveillance of VBDs in horses and can support public health decision making. It also, however, highlights the need to improve data collection and data sharing, to implement full performance assessments of complex surveillance systems, and to use effective communication and training to promote the adoption of these approaches.

Submitted by Caroline Falcao (caroline.rfalcao@ufpe.br) on 2017-06-19T17:21:04Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) 2012-Tese-GlaubiaArrudaSilva.pdf: 6824713 bytes, checksum: 821241186cb31ac2e399105bacd70cde (MD5) / Made available in DSpace on 2017-06-19T17:21:04Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) 2012-Tese-GlaubiaArrudaSilva.pdf: 6824713 bytes, checksum: 821241186cb31ac2e399105bacd70cde (MD5) Previous issue date: 2012-08-13 / Os estudos e as pesquisas históricas acerca das doenças constituem-se em caminhos por meio dos quais é possível construir novas perspectivas de análise das sociedades em tempos e espaços diversos. Essa tese de doutorado acerca da epidemia de malária, ocorrida entre os anos de 1937 e 1942, tem como um dos seus enfoques centrais a análise de como a população dos municípios localizados na área denominada Baixo Jaguaribe, no estado do Ceará, vivenciou este surto epidêmico. Outro caminho perseguido foi o de analisar os momentos em que a malária deixava de ser apenas um problema do indivíduo, da família e tornava-se alvo de políticas públicas dos governos municipal, estadual e federal, além disso, passavam também a ser negociadas com uma instituição dos EUA, a Fundação Rockefeller. Dessa forma, outro foco de análise foramas ações empreendidas pelo governo municipal, estadual, federal e pela Fundação Rockefeller nas tentativas de erradicar o mosquito transmissor da doença, Anopheles gambiae,através, por exemplo, de campanhas como o Serviço de Obras Contra a Malária (SOCM) e, posteriormente, pelo Serviço de Malária do Nordeste (SMNE). E, por fim, outra dimensão pesquisada e analisada nessa tese foram as relações estabelecidas entre os moradores locais eum saber institucionalizado pela ciência no combate a doença, confrontando, assim, os tratamentos e os saberes daquela população. / Studies andhistorical researchconcerning thediseases arepathsthrough whichone canbuild newperspectives on society’s analysisindifferenttimes and places. Thisdoctorate thesison themalaria epidemic, which occurred between1937 and1942,has asone of itscentralfocusesthe analysison how thepopulation of the municipalitieslocatedin the area calledBaixo Jaguaribein the state ofCeará,experiencedthisoutbreak. Anotherpath pursuedwas to analyzethe moments in whichmalariawas no longerjust a problem ofthe individual or its familyand becamethe target ofpublic policiesof municipal,state and federal government, also beingnegotiated withaU.S. institution, the Rockefeller Foundation.Thus, another focus of the present analysis was the actions taken by the municipal, state and federal government, and the Rockefeller Foundation in attempts to eradicate the mosquito that transmits the disease, Anopheles gambiae, through, for example, the campaigns such as the Serviço de Obras Contra a Malária (SOCM) and later by theServiço de Malária do Nordeste (SMNE). Finally, another dimension researched and analyzed in this thesis was the relation between local residents and a scientific institutionalized knowledge to fight the disease, thus comparing the treatments and knowledge of that population.

The subject of this diploma thesis is online marketing communication using search engine marketing tools. It specifies what online marketing and search engine marketing are, describes its tools and explains its use. The analytical part of the work uses tools such as analysis of macro and micro environment, analysis of marketing mix and associated SEO analysis. Last but not least, the development of website traffic and the subsequent possible development of this data in the future will also be examined. Based on the findings from the analytical part, in the last part of the work will be designed online marketing communication using search engine marketing.

MACHÁTOVÁ, M. Risk Assessment of Trading on International Markets. Diploma thesis. Brno: 2014. This Diploma thesis deals with the analysis of risks and benefits that may occur when trading in selected international markets. The aim of this thesis is to suggest appropriate measures to eliminate risks. In the teoretical part, problem of international trade is defined and important concepts of the risk management are explained. Later on a concrete enterprise and its entrepreneurship are introduced. The practical part analyzes the internal and external environment of the company and its market position compared to the competition. Furthermore the situation on the market is evaluated in economic, political, legal and cultural perspective. Various economic and risk-management methods are beeing used for the evaluation. Based on the results of analysis the markets are ranked from the most suitable to the least suitable for international trade.