Submitted by Marcia Silva (marcia@latec.uff.br) on 2016-05-31T20:17:29Z No. of bitstreams: 1 Dissert Chrystian Lopes da Silva.pdf: 2912107 bytes, checksum: 9aa2bd0c9ef0cd350da43cb060444799 (MD5) / Made available in DSpace on 2016-05-31T20:17:29Z (GMT). No. of bitstreams: 1 Dissert Chrystian Lopes da Silva.pdf: 2912107 bytes, checksum: 9aa2bd0c9ef0cd350da43cb060444799 (MD5) Previous issue date: 2015-12-14 / A Lei de Incentivo ao Esporte (Lei nº 11.438/2006) estabelece uma política pública de fomento ao esporte através de incentivos fiscais. A pesquisa analisa a efetividade da Lei na Costa do Sol/RJ, considerando a importância econômica da região em face da baixa utilização dos recursos incentivados pela Lei de Incentivo ao Esporte em projetos esportivos. O estudo fundamenta-se em duas vertentes: uma teórica, suportada pela revisão da literatura e outra empírica que analisa a compreensão da Lei de Incentivo ao Esporte a partir dos segundo e terceiro setores socioeconômicos. Os resultados sugerem que a efetividade da Lei de Incentivo ao Esporte está relacionada com o grau de aproximação dos três setores da sociedade. Palavras-chave: Lei de Incentivo ao Esporte (Lei nº 11.438/2006). Política pública de fomento ao esporte. Efetividade. / The Sports Incentive Law (Law 11.438/2006) establishes a public policy of promoting sport through tax incentives. The research analyzes the effectiveness of the Law in the Costa do Sol/RJ, considering the economic importance of the region in view of the low use of resources encouraged by the Sports Incentive Law in sports projects. The study is based on two components: theoretical, supported by literature review, and empirical that analyzes the understanding of the Sports Incentive Law based in the second and third socio-economic sectors. The results suggest that the effectiveness of the Sports Incentive Law is related to the degree of approximation of the three sectors of society. Keywords: Sports Incentive Law (Law 11.438/2006). Public policy of promoting sport. Effectiveness.

Orientador: Marcelo Carvalho Minhoto Teixeira Filho / Resumo: Alternative management practices are needed to minimize the need for chemical fertilizer use in non-leguminous cropping systems. The use of biological agents which can fix atmospheric nitrogen and promote plant growth has shown potential as an option to improve soil nutrient availability to grass crops. In recent years, studies investigating the effects of silicon (Si) have increased substantially, especially in grain crops. This increased interest in Si is likely due to the beneficial effects of Si application on plant resistance to abiotic and biotic stresses, reflecting on greater plant development. This research was developed to investigate if inoculation of corn and wheat with Azospirillum brasilense associated with Si can enhance nitrogen use efficiency (NUE) and improve plant nutrition and yield, leading to a greater economic profit. The study was set up in a Rhodic Haplustox under no-till system, located in Selvíria, state of Mato Grosso do Sul, Brazil. The field trial took place during the 2015/16 (November-March), 2016 (April-September), 2016/17 (November-March) and 2017 April-September) seasons, with corn and wheat crops in sucession (spring/summer and winter seasons for corn and wheat, respectively). Treatments were tested in a randomized completely block design with four replicates, arranged in a full factorial design 5 × 2 × 2 and included: i) five N application rates, as urea source, applied in side-dressing (0, 50, 100, 150 and 200 kg ha-1); ii) two liming sou... (Resumo completo, clicar acesso eletrônico abaixo) / Doutor

Bakgrund och problemformulering: I hälso- och sjukvårdsorganisationer är konflikter oundvikliga, ständigt förekommande och en naturlig del av arbetsplatsen. Ökad ohälsa i form av arbetsrelaterade sjukdomar och stress kan härledas till konflikter på arbetsplatsen. Trots det upplever många chefer ett obehag och svårigheter med hur konflikter ska hanteras. Syfte: Sammanställa aktuell forskning och få en samlad bild av olika konflikthanteringsstrategier med relevans för hälso- och sjukvårdsadministration under åren 2010-2021. Detta för att bidra till en hållbar hantering av mellanmänskliga konflikter inom hälso- och sjukvård för att främja en hälsofrämjande arbetsmiljö. Metod: Systematisk litteraturstudie med kvalitativ ansats. Analysen utfördes genom att syntetisera och sammanställa innehållet i tolv vetenskapliga primärstudier med både kvalitativ och kvantitativ ansats. Resultat: Studiens resultat indikerar att det finns ett orsakssamband mellan demografiska faktorer och val av konflikthanteringsstrategi. Vidare visar resultatet på att det finns en medvetenhet hos chefer om att en kompromissande, integrerande och aktiv konflikthantering huvudsakligen är det mest effektiva, men vissa studier indikerar att undvikande och dominerade strategier är förekommande. Resultatet visar även att chefer använder sig utav flera olika konflikthanteringsstrategier. Diskussion: Vi anser att konflikthantering bör vara ett prioriterat område för chefer som arbetar i verksamheter inom vård och omsorg för de tydligt positiva effekterna en effektiv konflikthantering har på hälsa för anställda, ökad effektivitet och vårdkvalitet. Samtidigt saknas det studier baserat på svensk kontext, orsakssamband och val av konflikthanteringsstrategier samt koppling till arbetsmiljö. Därför anser vi det nödvändigt med ytterligare forskning för att nå och möjliggöra ett hållbart och hälsofrämjande ledarskap. / Background: In health care organizations, conflicts are inevitable, constantly occurring and a natural part of the workplace. Decreased health in the form of occupational illnesses and stress can be attributed to unhandled workplace conflicts. Despite this, many nurse managers experience discomfort and difficulties with how conflicts should be handled. Aim: This study aimed to compile current research and present an overall picture of different conflict management strategies relevant to health care administration during the years 2010-2021. This is to contribute to the sustainable management of interpersonal conflicts in health care in order to promote a health-promoting work environment. Method: Systematic literature study with a qualitative approach. The analysis was performed by synthesizing and compiling twelve primary scientific studies with both a qualitative and quantitative approach. Results: Our result indicates a causal relationship between demographic factors and the choice of conflict management strategy. Furthermore, the results show that there is an awareness among managers that compromising, integrative and active conflict management generally is the most effective, but some studies indicate that avoidant and dominated strategies is prevalent. The results also show that managers use several different conflict management strategies. Conclusion/Discussion: Effective conflict management positively affects occupational health, organizational efficiency, and the quality of care. Therefore, we believe that conflict management should be a priority area for managers and management training in healthcare. There is a lack of studies based on the Swedish context, causation and choice of conflict management strategies, and connection to occupational health. Further research is needed to achieve and enable sustainable and health-promoting leadership.

Hälso- och sjukvården har under 2000-talet genomgått kraftiga organisatoriska förändringar samtidigt som det ställts ökade krav på högkvalitativ vård. Detta påverkar anställdas arbetssituation och kan leda till ökad arbetsbelastning och stress. Forskning visar att en god organisatorisk och social arbetsmiljö bidrar till välbefinnande, trivsel och välmående. Något som får anställda att stanna kvar. Tidigare forskning samt arbetsgivares insatser har i huvudsak fokuserat på risker och negativa faktorer som bidrar till sjukskrivningar. Ett hälsofrämjande perspektiv, där organisationen istället lägger fokus på det som främjar det goda på arbetsplatsen, skapar välbefinnande och främjar organisatorisk och social hållbarhet över tid. Hälsofrämjande arbetsmiljöarbete behöver ske i flera dimensioner där den psykosociala arbetsmiljön är fokus för välbefinnandet. Organisatorisk och social arbetsmiljö kännetecknas av faktorer som till skillnad mot fysisk arbetsmiljö är subjektivt abstrakta och som behöver omfattas av det hälsofrämjande arbetet. Denna studie sammanställer kunskapsläget kring vad som skapar en hälsofrämjande organisatorisk och social arbetsmiljö inom hälso- och sjukvård genom en systematisk litteraturstudie. Studiens huvudfynd visade att organisatoriska förutsättningar tillsammans med resurser som socialt stöd, autonomi, meningsfullhet är nyckelfaktorer till en hälsofrämjande arbetsmiljö. Dessa samverkar och överlappar varandra. De påverkar och påverkas av varandra både enskilt och tillsammans. Detta argumenterar för att det finns många vinster för organisationer att investera i anställdas välbefinnande. Hälsofrämjande organisatoriskt och socialt arbete behöver ske inom organisationen som ram, eftersom det utgör en determinant för anställdas välbefinnande och trivsel. Organisationen behöver stötta individens autonomi och även erbjuda socialt stöd och andra stödjande funktioner. Organisationen behöver också delaktiggöra medarbetare och hjälpa dem att öka sin känsla av meningsfullhet. / During the 2000s, the health service has undergone major organizational changes at the same time as they have faced increased demands for high-quality care. This affects the employees' work situation and can lead to increased workload and stress. Research shows that a good organizational and social work environment contributes to well-being. Something that makes people stay. Previous research and employers' efforts have mostly focused on risks and negative factors that contribute to sick leave. A health-promoting perspective, where the organization focuses on what promotes the good in the workplace, creates well-being and promotes organizational and social sustainability over time. Health-promoting environment work needs to take place in several dimensions where the psychosocial work environment is the focus for well-being. Organizational and social work environment is characterized by factors which, unlike the physical work environment, are subjectively abstract and which need to be included in the health promotion work. This study compiles the state of knowledge about what creates a health-promoting organizational and social work environment in health care, through a systematic literature study. The findings that were made were that Organizational conditions and workplace-related resources such as social support, autonomy, purpose (meaningfulness) are key factors in a health-promoting work environment. These interact and overlap systematically. They affect and are influenced by each other both individually and together. This argues that there are many benefits for organizations in investing in employee well-being. Health-promoting organizational and social work must take place within the organization as it constitutes a determinant of employee well-being and well-being. The organization needs to support the individual's autonomy and also offer social support and other supportive functions. The organization needs to involve employees and help them increase their sense of meaning.

Some microorganisms are known to form mutualistic symbiosis with plant roots and by their impact they can improve some plant parameters. These symbiotic microorganisms, which are able to improve some plant parameters, include especially mycorrhizal fungi, plant growth promoting bacteria and some saprotrophic mycoparasitical fungi. Mechanisms of changes of these parameters, as influenced by symbiotic microorganisms, are known only in part and nowadays are being actively researched. Aims of this work were to find out if selected microbial treatments influence selected growth, physiological and yield parameters of plants and contents of selected substances in fruits. Within this work were made three pot greenhouse experiments (experiments 1, 2 and 3) and three pilot greenhouse experiments (experiments 4, 5 and 6), performed on tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) plants. Plants were grown in hydroponics using a carrier of rockwool and they were watered by nutrient solution. As microbial treatments for plants in experiments have been used a mixture of arbuscular mycorrhizal fungi (AM), mixture of plant growth promoting bacteria (PGPB), saprotrophic mycoparasitical fungus Trichoderma harzianum (Th) and various mutual combinations of these treatments. There have been observed...

Le virus de l’immunodéficience humaine de type 1 (VIH-1) est l’agent étiologique du SIDA, un rétrovirus complexe encodant les protéines accessoires : Nef, Vif, Vpr et Vpu. La fonction principale de ces protéines est de moduler l’environnement cellulaire afin de promouvoir la réplication virale. Les travaux présentés dans cette thèse portent sur la protéine virale Vpr, une protéine bien connue pour son activité d’arrêt du cycle cellulaire en phase G2/M dans les cellules en division et pour l’avantage réplicatif qu’elle confère au virus durant l’infection de cellules myéloïdes. Les évènements sous-jacents à ces deux activités restent pour l’heure mal compris. Le but des travaux regroupés dans cet ouvrage est d’identifier de nouveaux facteurs cellulaires pouvant éventuellement expliquer les activités de Vpr précédemment décrites. Pour ce faire, nous avons utilisé une approche d’identification des partenaires de proximité par biotinylation, appelée BioID. L’avantage du BioID est de permettre un marquage in cellulo des protéines à proximité de la protéine d’intérêt. La mise en place et la caractérisation de cette approche font l’objet de la première section de cette thèse. En utilisant cette approche, nous avons défini un réseau de 352 partenaires cellulaires de la protéine Vpr. Parmi ces partenaires de Vpr, plusieurs sont organisés sous forme de complexes ou réseaux protéiques incluant notamment le complexe promoteur de l’anaphase/cyclosome (APC/C) et les centrosomes. Étant donné que le complexe APC/C est l’un des principaux régulateurs du cycle cellulaire, nous avons décidé d’analyser sa relation avec Vpr. Nous avons découvert que Vpr formait un complexe non seulement avec APC1, une sous-unité essentielle du complexe APC/C, mais aussi avec les coactivateurs (CDH1 et CDC20) de ce complexe. Nous avons par la suite démontré que Vpr induisait la dégradation d’APC1 et que celle-ci pouvait être prévenue par une double-mutation N28S-G41N de Vpr. Cette dégradation d’APC1 ne semblerait pas être reliée aux activités précédemment décrites de Vpr. Ces travaux font l’objet de la seconde section de cette thèse. Enfin, dans une troisième section, des travaux effectués en collaboration et analysant la relation entre les centrosomes et Vpr sont présentés. Cette thèse identifie 200 nouveaux partenaires de Vpr, ouvrant la porte à l’exploration de nouvelles cibles et activités de Vpr. Elle décrit également une nouvelle cible de Vpr : le complexe APC/C. Globalement nos résultats contribuent à une meilleure compréhension de la façon dont le VIH-1 manipule l’environnement cellulaire de l’hôte à travers la protéine virale Vpr. / Human immunodeficiency virus (HIV-1) is the AIDS causal agent. This complex retrovirus encodes several accessory proteins; namely Nef, Vif, Vpr and Vpu; whose functions are to manipulate the cellular host environment in order to favor HIV-1 viral replication. This thesis focused on Vpr whose main activities are to induce a cell cycle arrest in the G2/M phase in dividing cells and to provide a replicative advantage to HIV-1 during infection of myeloid cells such as macrophages. The cellular mechanisms underlying these two activities are up to now misunderstood. The main goal of the work presented in this thesis is to identify new cellular factors that could potentially explain the previously described Vpr activities. To do so, we used the proximity labelling approach called BioID. The main strength of BioID is to tag in cellulo partners of the protein of interest. The development as well as optimization of the BioID approach is presented in the thesis first section. Using BioID, we defined a network containing 352 cellular partners in close proximity with the viral protein Vpr. Amongst these cellular partners, several were organized into protein complexes or networks such as the anaphase promoting complex/cyclosome (APC/C) or the centrosome. Given that APC/C is a cell cycle master-regulator, we analyzed the interplay governing Vpr and APC/C interactions. We first demonstrated that Vpr could form a complex containing the scaffolding subunit APC1. APC/C coactivators, namely CDH1 and CDC20, could also be found in association with Vpr. We next showed that Vpr was inducing APC1 degradation and that Vpr residues N28 and G41 were essential to this activity. Surprisingly, the APC1-Vpr interplay does not relate to previously described Vpr activities. This work is presented in the second section of this thesis. Lastly, in the third section, a work done in collaboration analyzed the interplay between Vpr and the centrosomes. In this thesis we identified 200 new potential partners of Vpr, opening the doors to discover novel Vpr targets and activities. This thesis also defined APC/C as new Vpr target. Taken together our results allow a better understanding on how HIV-1 modulates the cellular environment by using the viral accessory protein Vpr.

This thesis is a case study of measures promoting gender equality in RWE CZ company. Dissertation introduces Czech labor market reflecting the existence gender inequalities and defines their impact. Summarizes Czech institutional and legislative measures promoting gender equality on the national level. Empirical part focuses on the critical analyses of chosen measures from the gender perspective and suggests measures that could increase efficiency of current approaches adapted by RWE CZ company.

This dissertation is a case study of Česká spořitelna, a. s. It shows the significance of gender audit by asserting equal opportunities for women and men. The theoretical part introduces how gender equality could be asserted and also summarizes how the concept has (not) been practically fulfilled (particularly in the area of labour market). It emphasizes the gender audit as one of the possible tools of change when asserting the gender equality. The empirical part of this dissertation is investigating the significance of gender audit for asserting the equal opportunities in Česká spořitelna, a. s. Therefore it analyzes a final report of the first gender audit and compares its design with another final report that came out of the second implemented gender audit. It also analyzes the processes the company adopted after the first gender audit to promote the equal opportunities for women and men. The impacts and the efficiency of the measures (either proposed or implemented) are analyzed critically with regard to their influence on the gender equality.

L'intensification des activités industrielles et les besoins en énergie font des hydrocarbures pétroliers (HP) un enjeu majeur mondial mais augmentent aussi considérablement les risques environnementaux dans divers écosystèmes. La phytoremédiation est une phytotechnologie qui a fait ses preuves en tant que solution verte pour faire face aux contaminations des sols par des HP. La phytoremédiation des sols contaminés par les HP repose principalement sur l’activité des communautés microbiennes associées aux racines des plantes au niveau de la rhizosphère, qui peuvent non seulement favoriser la croissance des plantes hôtes mais aussi augmenter leur tolérance à divers stress biotiques et abiotiques. Parmi les défis majeurs de la phytoremédiation des sols contaminés par les HP, on compte la forte toxicité de certains composés des HP qui entravent la croissance des plantes et par conséquent l’efficacité de la phytoremédiation. Cependant, la croissance des plantes peut être positivement stimulée par la présence de rhizobactéries favorisant leur croissance (PGPR) qui sont capables d'atténuer le stress des plantes par divers mécanismes. Dans cette thèse, un total de 438 bactéries PGPR dégradant les hydrocarbures pétroliers, ont été isolées de la rhizosphère et du sol de deux espèces de plantes, Salix purpurea et Eleocharis obusta, dans un site d'une ancienne raffinerie pétrochimique à Varennes, QC, Canada. Les isolats bactériens ont été classés en 62 genres, appartenant aux phylums Actinobacteria, Bacteroidetes, Firmicutes et aux sous-groupes Alpha-, Beta- et Gamma-Proteobacteria. De plus, cette collection de cultures contient 438 isolats bactériens avec de multiples caractéristiques de dégradation et de stimulation de croissance (PGPR), représentant une diversité fonctionnelle de dégradation des HP et de caractéristiques PGPR qui pourraient être utilisées dans la phytoremédiation assistée par les bactéries, des sols contaminés par les HP. Parmi ces 438 isolats bactériens, 50 isolats représentant une large diversité taxonomique, ont été sélectionnées pour une caractérisation approfondie supplémentaire concernant leur capacité à favoriser la croissance des plantes en présence de différentes concentrations de n-hexadécane (0%, 1%, 2%, 3%) dans des conditions contrôlées. Les résultats ont indiqué que les isolats bactériens Nocardia sp. (WB46), Pseudomonas plecoglossicida (ET27), Stenotrophomonas pavanii (EB31), Bacillus megaterium (WT10) et Gordonia amicalis (WT12) ont significativement augmenté la croissance des plantes cultivées dans 3% de n-hexadécane par rapport au traitement témoin. De plus, ces isolats possèdent plusieurs traits favorisant la croissance des plantes (PGPR) tels que l'activité 1-aminocyclopropane-1-carboxylate (ACC) désaminase (ACCD), la production d'acide indole-3-acétique (IAA) et la fixation de l'azote. De plus, ces isolats étaient capables d'utiliser le n-hexadécane comme seule source de carbone et possédaient des gènes cataboliques liés à la dégradation des hydrocarbures tels que le gène de l'alcane monooxygénase (alkB), le cytochrome P450 hydroxylase (CYP153) et le gène de la naphtalène dioxygénase (nah1). Nocardia sp. isolate WB46, a été sélectionné pour le séquençage de son génome afin de déterminer sa diversité génétique et fonctionnelle relatives à la dégradation des HP et les potentiels PGPR. Les résultats ont indiqué que, sur la base des analyses du gène de l'ARNr 16S, l'hybridation ADN-ADN in silico (DDH) et l'identité moyenne des nucléotides (ANI), Nocardia sp. isolate WB46 représente une nouvelle espèce bactérienne. De plus, l'annotation fonctionnelle de son génome révèle que celui-ci contient de nombreux gènes responsables de la dégradation des hydrocarbures pétroliers tels que l'alcane 1-monooxygénase (alkB) et la naphtalène dioxygénase (ndo) ainsi que d'autres gènes liés à ses potentiels PGPR. En conclusion, la rhizosphère des espèces S. purpurea et E. obusta poussant dans un site fortement pollué par les HP représente un biotope diversifié et comprenant des bactéries PGPR avec de multiples potentiels de dégradation des HP. De plus, plusieurs isolats bactériens tels que Nocardia sp. (WB46), Pseudomonas plecoglossicida (ET27) et Stenotrophomonas pavanii (EB31) démontrent un potentiel d'utilisation comme bioinoculants pour de futures études de phytoremédiation à grande échelle. / Petroleum hydrocarbons (PHCs), as a result of intensification of industrial activities, are a global environmental issue especially in soil environments. Phytoremediation represents an ideal solution to tackle this global crisis. Phytoremediation of PHC-contaminated soils proceeds mainly through the activities of microbial communities that colonize the plant rhizosphere which might promote host plants growth and increase its tolerance to various biotic and abiotic stresses. A main challenge in phytoremediation of PHC-contaminated soils is the high toxicity of PHCs which hinder plant growth and reduce the efficiency of phytoremediation. However, plant growth may be positively stimulated by the presence of plant growth-promoting rhizobacteria (PGPR) that are able to alleviate stresses in plants through various mechanisms. In this thesis, a total of 438 petroleum hydrocarbons degrading-PGPR bacterial isolates were recovered from the rhizosphere and the surrounding bulk soil of Salix purpurea and Eleocharis obusta plants from the site of a former petrochemical plant in Varennes, QC, Canada. Bacterial isolates were classified into 62 genera, belonging to the phyla Actinobacteria, Bacteroidetes, Firmicutes and the Alpha, Beta and Gamma-subgroups of Proteobacteria. Additionally, this culture collection holds 438 bacterial isolates with multiple degradative and PGP features, representing a rich reservoir of metabolically versatile PGPR-PHC degraders that could be used in holistic, bacterial-aided phytomanagement of PHC-contaminated soils. Among the above 438 bacterial isolates, 50 bacterial strains representing a wide phylogenetic range were selected for an additional in-depth characterization regarding their ability to promote plant growth under the presence of different concentrations of n-hexadecane (0%, 1%, 2%, 3%) under gnotobiotic conditions. Results indicated that bacterial isolates Nocardia sp. (WB46), Pseudomonas plecoglossicida (ET27), Stenotrophomonas pavanii (EB31), Bacillus megaterium (WT10) and Gordonia amicalis (WT12) significantly increased the growth of plants grown in 3% n-hexadecane compared with the control treatment. Additionally, these isolates possess several plant-growth-promoting (PGP) traits such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD) activity, indole-3-acetic acid (IAA) production and nitrogen fixation. Also, these isolates were able to use n-hexadecane as sole source of carbon and have catabolic genes related to hydrocarbon degradation such alkane monooxygenase (alkB) gene, the cytochrome P450 hydroxylase (CYP153) and the naphthalene dioxygenase (nah1) gene. The isolate that showed the highest growth stimulation of plants grown in 3% n-hexadecane under gnotobiotic conditions, Nocardia sp. isolate WB46, was selected for de novo genome sequencing to unveil its genetic versatility and the mechanisms of PHCs biodegradation and PGP potentials. Results indicated that based on the 16S rRNA gene analyses, in silico DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) Nocardia sp. isolate WB46 is a new species. Additionally, the functional annotation of the genome of Nocardia sp. isolate WB46 reveals that its genome contains many genes responsible for petroleum hydrocarbon degradation such as alkane 1-monooxygenase (alkB) and naphthalene dioxygenase (ndo) as well as other genes related to its PGP potentials. In conclusion, S. purpurea and E. obusta growing in a site highly polluted with PHCs are rich reservoir of diverse PGPR with multiple PHC-degradation and PGP potentials. In addition, several bacterial isolates such as Nocardia sp. (WB46), Pseudomonas plecoglossicida (ET27) and Stenotrophomonas pavanii (EB31) demonstrate potential for use as bioinoculants in future large-scale phytoremediation studies.

Till följd av NPM (New public management) och de ökade kraven på produktivitet och effektivitet har sjuksköterskors arbetssituation försämrats alltmer under de senaste decennierna. Det har resulterat i en försämrad hälsa hos sjuksköterskorna och många sjuksköterskor har valt att lämna yrket. Det har bland annat genererat brist på erfarna sjuksköterskor på akutvårdsavdelningarna som bedrivs dygnet runt i regionerna och detta anser Socialstyrelsen utgöra ett hot mot patientsäkerheten. Ett bra verksamhetsnära ledarskap, i regionerna benämns dessa som första linjens chefer, är en betydande faktor för sjuksköterskors hälsa och vilja att stanna kvar på sin arbetsplats. Det är därför av intresse att undersöka vilka ledaregenskaper som krävs för att erfarna sjuksköterskor skall arbeta kvar på vårdavdelningar som är öppna dygnet runt. Syftet med studien är att utröna vad som är viktiga hörnstenar i det hälsofrämjande ledarskapet för att bibehålla erfarna sjuksköterskor i dygnet runt verksamhet. Som metod användes semistrukturerade kvalitativa intervjuer med en induktiv ansats, åtta intervjuer utfördes totalt. Råmaterialet analyserades med hjälp av innehållsanalys och meningsbärande enheter, koder, kategorier och teman identifierades. Resultatet utmynnade i tre teman: individuellt stöd, teamarbete och kommunikation. Informanterna lyfte bland annat individanpassade scheman, kompetensutveckling och temaarbete som viktiga aspekter för att kvarstanna på arbetsplatsen. Ett salutogent förhållningssätt och specifika hälsofrämjande ledarskapsegenskaper är grunderna för att bibehålla erfarna sjuksköterskor i verksamheter som bedrivs dygnet runt. Cheferna i en dygnet runt-verksamhet bör besitta följande förmågor: rak och tydlig kommunikation, förmåga att skapa tillit, vara lyhörda, kunna entusiasmera, prestigelöshet, vara drivna och målfokuserade samt inneha ett coachande förhållningssätt. Definition av erfarna sjuksköterskor, se sida 7 under Metod. / As a result of NPM (New public management) and the increased demands on productivity and efficiency, the work situation of nurses has deteriorated more and more in recent decades. This has resulted in a deterioration in the health of the nurses and a number of staff have chosen to leave the profession. Among other things, it has generated a shortage of experienced nurses in the Swedish emergency departments with twenty-four seven operations in the regions, and the Swedish National Board of Health and Welfare considers this to be a threat to patient safety. A leadership with the adjacent connection to the ward and the staff, are referred to as first-line managers in the Swedish regions. These mangers are significant factors for nurses' health and willingness to stay in their workplace. It is therefore of interest to investigate what leadership qualities are required for experienced1 nurses to continue working in wards that are open twenty-four seven. The purpose of the study is to find out what are important cornerstones in the health promoting leadership in order to retain experienced nurses in twenty-four seven operations. As a method, semi-structured qualitative interviews with an inductive approach were used, eight interviews were performed. The raw material was analyzed using content analysis and meaningful units, codes, categories and themes were identified. The result resulted in three themes: individual support, teamwork and communication. The informants highlighted, among other things, individualized schedules, skills development and team work as important aspects for staying in the workplace. The result was that specific health promoting leadership qualities and a salutogenic approach are the foundations for retaining experienced nurses in twenty-four seven operations. The managers in a twenty-four seven operations should possess the following abilities: straight forward and clear communication, the ability to create trust, be responsive, be able to enthuse, lack prestige, be driven and goal focused and have a coaching attitude. For a definition of experienced nurses, see page 7 under Method.