With World Health Organization estimation of population increase to over 9 billion by 2050, malnutrition and food insecurity are predicated to be two of the main challenges facing the world in the future. Fish is a source of protein that can have a significant contribution for solving the global food crisis. Fish for human consumption is provided by both the aquaculture industry and the capture fisheries, however, the flat-lined production of seafood from capture fisheries means aquaculture production should double in number to provide enough protein by 2050. Culturing fish at higher densities is being used as a way to increase the global aquaculture production. Modern aquaculture intensified practices expose the fish to various stressors that can negatively impact their welfare. Good nutrition can improve the health status of aquacultured fish while accumulation of specific nutrient can add value to the fillet. In line with that, the studies described in this dissertation aimed to examine the vital role of fish in human health and evaluate strategies to improve the sustainable aquaculture production of fish using novel aquafeeds. This was completed by conducting a survey of the essential minerals and heavy metals of commercially available catfish, salmon, and tilapia fillets in the United States marketplace and three fish nutritional trials to evaluate the impacts of novel aquafeeds on production and health of fish and the food quality of their respective fillets. Selenium with the established health benefit for human and fish was the trace mineral of interest in this dissertation. This element is the main component of a group of selenium containing proteins called selenoproteins that are antioxidant and maintain oxidative homeostasis of fish and human. Additionally, selenium is an antagonist for many heavy metals such as mercury. The market study revealed that catfish, salmon and tilapia samples available in United States marketplace are good source of selenium, zinc and phosphorous. The concentration of heavy metals in all the samples were lower than safety standards. Nile tilapia (Oreochromis niloticus) and rainbow trout (Oncorhynchus mykiss), which are two economically important aquacultured fish, were used as animal models in the fish nutritional trials. Different forms of selenium supplemented to Nile tilapia diet. The form of Dietary Se form did not have any significant (p>0.05) effect on growth, and plasma and hepatic GPX activity of Nile tilapia. However, organic selenium supplement showed significantly (p<0.05) higher bioavailability and accumulated in the fillets at the significantly higher concentration. In the second fish trial selenoneine, a novel selenium containing compound, was supplemented at various concentrations to rainbow trout diet. Other than growth parameters multiple other health biomarkers (lysozyme, glutathione peroxidase, superoxide dismutase activities) were examined to determine whether selenoneine supplementation had a positive impact on fish health. Selenoneine enriched diet significantly(p<0.05) increased the weight gain, lysozyme and oxidative enzymes activities of rainbow trout. Lastly rainbow trout feed was supplemented with commercially available probiotic (Actisaf®), prebiotic (Safmmann®), and their mixture (synbiotic). These dietary supplements did not have any significant effect on fish production but led to a significant increase in activity of oxidative enzymes in plasma when compared to control diet. These results suggest that feed supplements used in this dissertation can be used to promote the fish health raised in recirculating aquaculture systems and support an economically and environmentally sustainable aquaculture. / PHD / Fish contributes significantly to human’s diet in terms of high quality, easily digestible animal protein that helps fight malnutrition and food insecurity. More than half of the fish for human consumption is produced by the aquaculture industry and the growth of the industry is very critical for meeting the future needs for protein. Other than production of high-quality protein the aquaculture industry has made a great contribution to people’s livelihood, poverty alleviation, income generation, employment and, trade globally. To continue contributing effectively to global food security aquaculture industry should overcome various obstacles such as disease and sustainable production of high-quality feed. In this dissertation, important roles of fish in human diet was examined by measuring the mineral composition of commercially available catfish, salmon and tilapia in the United States market place. Also diets of Nile tilapia and rainbow trout were supplemented with various forms of selenium, prebiotics, and/or probiotics which have positive effect on health of both human and fish. These supplementations led to production of valued-added fish fillets enriched in selenium and improved the fish growth and health.

Orientador: Tsunao Matsumoto / Banca: Alexandre Ninhaus Silveira / Banca: Katt Reginal Lapa / Resumo: Sistemas de Recirculação para Aquicultura (SRA) possibilitam um meio alternativo de produção de peixes para áreas com limitada disponibilidade de água. Estes sistemas de cultivo intensivo de peixes oferecem potencial de produção em pequena escala assim como em grande escala, devido a recuperação de efluentes e a reutilização da água, produzindo volumes relativamente pequenos de resíduos líquidos e sólidos. O objetivo deste estudo foi determinar a eficácia do tratamento de águas residuárias em relação à remoção de Nitrogênio Amoniacal Total (NAT), Sólidos Totais (ST), Sólidos Suspensos Totais (SST), Demanda Química de Oxigênio (DQO) e Demanda Bioquímica de Oxigênio (DBO5), Nitrogênio total (NT) e Fósforo Total (FT). Para tanto, foram introduzidos Tilápia do Nilo (Oreochromis niloticus), com 0,323kg de peso médio inicial e 17 kg m-3 de densidade de armazenamento inicial no SRA experimental. O SRA era composto de 03 tanques de plástico com capacidade de 0,20 m3, Decantador de Coluna (DC), o reator de leito fluidizado aeróbio com circulação em tubos concêntricos (BAS-CT), tendo como meio de suporte a areia de diâmetro efetivo (D10) = 0,29 mm e na sequência reator de transferência de oxigênio e remoção de CO2. A eficiência na remoção do NAT no reator BAS-CT no período de aclimatação e de estabilização foi 31,0% e 35,2%, respectivamente. A remoção de ST e SST foi de 44,44% e 71,71%, respectivamente. A eficiência alcançada de remoção no SRA da DQO foi de 64,90% e de 48,01% para DBO5. Os valores médios de remoção do NT e FT no SRA foram de 25,02% e 41,05%, respectivamente. Estes resultados indicaram que o tratamento de águas residuárias de aquicultura proposto neste estudo através do BAS-CT associado ao DC tem evitado elevadas concentrações e acumulação de poluentes dentro do SRA / Abstract: Recirculating aquaculture systems (RAS) offer an alternative means of fish production for areas that to have limited water and land availability. RAS are a highly intensive fish culture systems and offer potential production units for small and large scale operations. For effluent recovery and treated water reuse, RAS produce relatively small volumes of liquid and solids wastes. The objective of this study were to determine the efficiency of the wastewater treatment with recommended to removal of Total Ammonia Nitrogen (TAN), Total Solids (TS), Total Suspended Solids (TSS), Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD5), Total Nitrogen (TN) and Total Phosphorus (TP). In this study was cultivating the Nile Tilapia (Oreochromis niloticus) with 0.323kg initial average weight and 17 kg m-3 initial storage density, cultivated in RAS. The RAS was composed with 03 plastic tanks with capacity of 0.20 m3 in each, column settler (CS), aerobic fluidized bed reactor with concentric tubes circulation (BAS-CT) with sand bed support of effective diameter (D10) of 0.29 mm and oxygen transfer-CO2 removal reactor. NAT removal efficiency was in acclimation and stabilized periods in BAS-CT reactor were 31.0% and 35.2% respectively. TS and TSS removal was 44.44% and 71.71% respectively. In this RAS, the COD removal efficiency reached was 64.90% and 48.01% for BOD5. The average removal in the NT and FT in the RAS were 25.02% and 41.05% respectively. These results indicate that aquaculture wastewater treatment proposed in this study by BAS-CT associated to CS prevents high concentrations and accumulating of pollutants within the SRA

Integrated aquaculture systems (IAS) are a type of recirculating aquaculture systems (RAS) where the wastewater is treated and returned to the fish tanks. The important difference between the two is that in an IAS, wastes from the aquaculture component are recovered as fertilizer to produce an agricultural product whereas in an RAS, waste organics, nutrients and solids are treated and discharged. A pilot marine IAS at Mote Aquaculture Research Park in Sarasota, FL was studied for this project. Water quality monitoring, measurements of fish health and growth rates of fish and plants were performed over a two-year period to determine the effectiveness of the system in producing fish and plant products and removing pollutants. The goal of this portion of the project was to develop, calibrate and evaluate a model of the system, to understand the nitrogen transformations within the Mote IAS and to investigate other potential configurations of the Mote IAS. The model was divided into the various compartments to simulate each stage of the system, which included fish tanks, a drum filter for solids removal, and moving bed bioreactor (MBBR) for nitrification and disinfection. A solids tank after the drum filter was used to store the drum filter effluent slurry, which was then divided between three treatment processes: a geotube, a sand filter followed by a plant bed, and a plant bed alone. Nitrogen species modeled were particulate organic nitrogen (PON), dissolved organic nitrogen (DON), ammonium and nitrate. Of the physical components of the IAS, models of the MBBR and the two plant raceways included physical, chemical and biological nitrogen transformation processes. The sand filter, solids tank and geotube models were simple mass balances, incorporating factional removals of each species based on the observed data. Other variables modeled included temperature, dissolved oxygen, volatile suspended solids and chemical oxygen demand concentrations. The model was built in a computer program, STELLATM, to simulate the Mote IAS. The model calibration involved experimental, literature and calibrated parameters. Parameters were adjusted until the model's output was a best fit to the observed data by minimizing the sum of the squared residuals. During the sensitivity analysis, two model parameters caused large variations in the model output. The denitrifier constant caused the most variation to the model's output followed by the denitrifier fraction of volatile suspended solids. Of the removal processes, denitrification was the largest nitrogen removal mechanism from the model, accounting for 59% and 55% of the nitrogen removed from the south and north plant raceways respectively. Plant and soil uptake represented only 0.2% of the overall nitrogen removal processes followed by 0.1% by sedimentation. Finally, the model was used to investigate other treatment designs if the Mote IAS was redesigned. The first option involved a geotube and one plant raceway in series to treat the solid waste while the second option did not have a geotube, but two plant raceways. The first option was the most effective at removing nitrogen while the second was as effective as the original system and would cost less.

No description available.

Removal of nitrogen elements in culture water is one of the major concerns in recirculating aquaculture system (RAS). Maintaining a low concentration of nitrogen compounds is essential for a good quality of aquaculture production. Due to fish is very sensitive to the toxic ammonium/ammonia, nitrification biofiltration tank is often an integrate part of filtration in RAS to remove ammonium via nitrification. However, nitrate accumulation via nitrification in RAS is often observed during the operation, which is usually solved by replacing with the fresh water into the system. With the concern of water consumption, bio-electrochemical system (BES) is introduced in this study to realize simultaneous nitrate removal for the system while generating the electricity through electron transferring. A microbial fuel cell (MFC) with an anion exchange membrane (AEM) was constructed. The removal of nitrate from aquaculture water generated from RAS was achieved by nitrate migration across the AEM and heterotrophic denitrification in the anode chamber. To further investigate the potential application of BES in RAS, the cathode chamber was incubated with biofilm to do the nitrification while the denitrification processing in the anode chamber. The study gave a total inorganic nitrogen removal efficiency of 38.72% ± 4.99, and a COD removal of 86.09% ± 9.83. The average daily electricity generation was 67.98 A m-3 ± 13.91, and nitrate-nitrogen concentration remained at 21.02 ± 2.62 mg L-1 throughout the experiment. These results of treating aquaculture water indicate that BES has a potential to install within RAS for enhanced nitrogen removal. / MS

Fler svenskar vill konsumera mer inhemskt producerad fisk. Scandinavian Aquasystems odlar och försöker introducera fisken tilapia på svenska marknaden. Tilapia är en fisk som precis börjat göra intåg i Europa men är stor runtomkring resten av världen. En smakprofil som berättar hur länge produkten är sensoriskt tjänlig kan vara av hjälp. Denna undersökning syftade till att skapa en sensorisk smakprofil för fisken tilapia och till att se hur smakprofilen förändrades vid lagring i vakuumförpackning. För att skapa smakprofilen användes metoden QDA. Resultatet gav många egenskapsord var av vissa kunde klassificeras som färskhetsrelaterade och vissa som förskämningsrelaterade. Det kunde även konstateras att färsk, odlad tilapia har en antydan av saftigare och mer mjäll textur. Vid lagring finns en möjlighet att tilapia får en intensivare smak av dy och metall, samt en intensivare doft av dy. / A large amount of Swedes has a desire to consume more nationally produced fish. Scandinavian Aquasystems produces and tries to introduce tilapia into the Swedish market. As a fish, tilapia has just begun making ways into Europe but is already a big commodity around the rest of the world. In addition, a sensory profile which tells how long the product remains sensorially suitable could be of help. This study aimed to create a sensory profile and to study its inherent effects of vacuum packaging over time. In creating the sensory profile, a QDA method was used. The study resulted in a variance of different attributes which of some could be categorized as either fresh related or spoilage related. It was found that fresh, farmed tilapia carried an indication of having a juicier and more tender texture. With storage there is chance of tilapia getting a more intense flavor of mud and metal, as well as a more intense odor of mud.

Made available in DSpace on 2014-06-11T19:30:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-08-22Bitstream added on 2014-06-13T20:20:51Z : No. of bitstreams: 1 maigualenriquez_ya_me_ilha.pdf: 1515184 bytes, checksum: 854b45266358b64c6017c5058af88c81 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Sistemas de Recirculação para Aquicultura (SRA) possibilitam um meio alternativo de produção de peixes para áreas com limitada disponibilidade de água. Estes sistemas de cultivo intensivo de peixes oferecem potencial de produção em pequena escala assim como em grande escala, devido a recuperação de efluentes e a reutilização da água, produzindo volumes relativamente pequenos de resíduos líquidos e sólidos. O objetivo deste estudo foi determinar a eficácia do tratamento de águas residuárias em relação à remoção de Nitrogênio Amoniacal Total (NAT), Sólidos Totais (ST), Sólidos Suspensos Totais (SST), Demanda Química de Oxigênio (DQO) e Demanda Bioquímica de Oxigênio (DBO5), Nitrogênio total (NT) e Fósforo Total (FT). Para tanto, foram introduzidos Tilápia do Nilo (Oreochromis niloticus), com 0,323kg de peso médio inicial e 17 kg m-3 de densidade de armazenamento inicial no SRA experimental. O SRA era composto de 03 tanques de plástico com capacidade de 0,20 m3, Decantador de Coluna (DC), o reator de leito fluidizado aeróbio com circulação em tubos concêntricos (BAS-CT), tendo como meio de suporte a areia de diâmetro efetivo (D10) = 0,29 mm e na sequência reator de transferência de oxigênio e remoção de CO2. A eficiência na remoção do NAT no reator BAS-CT no período de aclimatação e de estabilização foi 31,0% e 35,2%, respectivamente. A remoção de ST e SST foi de 44,44% e 71,71%, respectivamente. A eficiência alcançada de remoção no SRA da DQO foi de 64,90% e de 48,01% para DBO5. Os valores médios de remoção do NT e FT no SRA foram de 25,02% e 41,05%, respectivamente. Estes resultados indicaram que o tratamento de águas residuárias de aquicultura proposto neste estudo através do BAS-CT associado ao DC tem evitado elevadas concentrações e acumulação de poluentes dentro do SRA / Recirculating aquaculture systems (RAS) offer an alternative means of fish production for areas that to have limited water and land availability. RAS are a highly intensive fish culture systems and offer potential production units for small and large scale operations. For effluent recovery and treated water reuse, RAS produce relatively small volumes of liquid and solids wastes. The objective of this study were to determine the efficiency of the wastewater treatment with recommended to removal of Total Ammonia Nitrogen (TAN), Total Solids (TS), Total Suspended Solids (TSS), Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD5), Total Nitrogen (TN) and Total Phosphorus (TP). In this study was cultivating the Nile Tilapia (Oreochromis niloticus) with 0.323kg initial average weight and 17 kg m-3 initial storage density, cultivated in RAS. The RAS was composed with 03 plastic tanks with capacity of 0.20 m3 in each, column settler (CS), aerobic fluidized bed reactor with concentric tubes circulation (BAS-CT) with sand bed support of effective diameter (D10) of 0.29 mm and oxygen transfer-CO2 removal reactor. NAT removal efficiency was in acclimation and stabilized periods in BAS-CT reactor were 31.0% and 35.2% respectively. TS and TSS removal was 44.44% and 71.71% respectively. In this RAS, the COD removal efficiency reached was 64.90% and 48.01% for BOD5. The average removal in the NT and FT in the RAS were 25.02% and 41.05% respectively. These results indicate that aquaculture wastewater treatment proposed in this study by BAS-CT associated to CS prevents high concentrations and accumulating of pollutants within the SRA

Made available in DSpace on 2016-06-02T19:29:53Z (GMT). No. of bitstreams: 1 4560.pdf: 1880275 bytes, checksum: 259fd9d9b3aeb99130c54c0ee0f5af82 (MD5) Previous issue date: 2012-05-31 / This study aimed to test a pilot recirculating aquaculture system (RAS) and to evaluate in this system the effects of the stocking density on the production of species of surubim pintado , Pseudoplatystoma corruscans, and its hybrid with P. reticulatum regarding the effect of stocking density and water renewal rate on the hybrid production. The study lasted for 84 days. The physical and chemical conditions in the RAS was evaluated by measuring water consumption (223,6 L/kg of fish produced), temperature (25,8±0,7ºC), dissolved oxygen (5,33±0,04 mg/L O2 in the water inlet to culture tanks), pH (6,75±0,25), total alkalinity (40,0±20,6 mg/L CaCO3), carbon dioxide (16,6±6,1 mg/L CO2), total suspended solids (8,0±4,2 mg/L TSS), total ammonia nitrogen (0,18±0,10 mg/L NH3-N), nitrite (0,163±0,090 mg/L NO2-) and nitrate (58,0±9,7 mg/L NO3-). To evaluate the fish performance, two experiments were conducted simultaneously. In experiment 1, densities of 40, 80 and 120 fish.m-3 (approximately 8.5, 17 and 25.5 kg.m-3) for the pure and the hybrid lineages were tested using a 3 x 2 factorial design (three densities and two lineages). In experiment 2, only the hybrid lineage was used, and three stocking densities were tested: 20, 40 and 60 fish.m-3 (approximately 9, 18 and 27 kg.m-3) and two water renewal rates (1 L/kg.min-1 and 0,5 L/kg.min-1), using a 3 x 2 factorial design (three densities and two water renewal rates). Fish were fed with extruded food (40% crude protein) at a rate of 2.5% of the body weight per day. For both experiments, daily specific growth rate (SGR), final weight, biomass gain, final density, food conversion, final weight and dissolved oxygen levels at the tank outlet are discussed. In experiment 1, both lineages showed to be adapted to the RAS, but the hybrid lineage had a better performance than the pure lineage. In experiment 2, the highest water renewal rate resulted in a better performance, and an increase in density improved the final production. / O objetivo deste trabalho foi testar um sistema piloto de recirculação de água (SRA) e avaliar neste sistema o efeito da densidade de estocagem no cultivo de surubins puros, Pseudoplatystoma corruscans, e do seu híbrido (P. reticulatum x P. corruscans) e o efeito da densidade de estocagem e da taxa de renovação de água no cultivo do híbrido. O trabalho teve duração de 84 dias. O SRA foi avaliado através das medições de consumo de água (223,6 L/kg de peixe produzido), temperatura (25,8±0,7ºC), oxigênio dissolvido (5,33±0,04 mg/L de O2 na água de abastecimento dos tanques de cultivo), pH (6,75±0,25), alcalinidade total (40,0±20,6 mg/L CaCO3), gás carbônico (16,6±6,1 mg/L CO2), sólidos suspensos totais (8,0±4,2 mg/L SST), nitrogênio amoniacal total (0,18±0,10 mg/L NH3-N), nitrito (0,163±0,090 mg/L NO2-) e nitrato (58,0±9,7 mg/L NO3-). Para avaliação do desempenho dos peixes, foram realizados dois experimentos simultaneamente. No experimento 1 foram testadas as densidades de 40, 80 e 120 peixes/m3 (aproximadamente 8,5, 17 e 25,5 Kg/m³) para as linhagens pura e híbrida, num delineamento em fatorial 3 × 2 (três densidades e duas linhagens). No experimento 2 foi utilizada somente a linhagem híbrida, tendo sido testadas 3 densidades de estocagem, 20, 40 e 60 peixes/m3 (aproximadamente 9, 18 e 27 Kg/m³) e duas taxas de renovação de água (1 L/kg.min-1 e 0,5 L/kg.min-1), num delineamento em fatorial 3 × 2 (três densidades de estocagem e duas taxas de renovação de água). A alimentação dos peixes nos dois experimentos foi realizada com ração extrusada (40% de proteína bruta) na proporção de 2,5% da biomassa, ministrada diariamente. Para os dois experimentos foram discutidos dados de taxa de crescimento específico diária, peso final, ganho de biomassa, densidade final produzida, conversão alimentar, peso final e níveis de oxigênio dissolvido na saída dos tanques. No experimento 1, foi observado que as duas linhagens se adaptaram ao SRA, porém a linhagem híbrida teve melhor desempenho que a linhagem pura. No experimento 2, a maior taxa de renovação de água proporcionou melhor desempenho e o aumento de densidade, incrementando a produção final.

In der Aquakultur sind Kreislaufanlagen ein umweltfreundliches und wassersparendes Produktionsverfahren. Hohe Besatzdichten und das Prinzip der Wasserführung im Kreislauf führen jedoch auch zu einem erhöhten Risiko von Infektionskrankheiten. In dem hier beschriebenen Projekt wurde untersucht, wie sich niederfrequenter Ultraschall (nf-US) in Kombination mit der in der Aquakultur bewährten UV-C Bestrahlung zur Kontrolle von Pathogenen einsetzen lässt. Es wurden vergleichende Untersuchungen zur Effizienz von nf-US, UV-C und deren Kombination gegen prokaryotische und eukaryotische Modellorganismen durchgeführt. Während sich UV-C als sehr effektiv gegen Bakterien erwies, konnte die Gesamtkeimzahl mit nf-US nicht reduziert werden. Eine Vorbehandlung des Wassers mit nf-US verringerte jedoch die mittlere Größe der im Wasser suspendierten Partikel und konnte so die Effektivität von UV-C zur Inaktivierung von Bakterien verbessern. Zur Abtötung eukaryotioscher Organismen wird eine deutlich höhere UV-Dosis als zur Kontrolle von Bakterien benötigt. Eine starke Erhöhung der UV-C Dosis ist jedoch durch die dann mögliche photo-induzierte Bildung von Nitrit aus Nitrat limitiert. Alternativ könnte nf-US zur Abtötung eukariotischer Parasiten verwendet werden. Unterschiedlichen Organismen unterscheiden sich stark in ihrer Empfindlichkeit gegenüber nf-US, wobei sich die dosisabhängige Abtötung sehr gut mit Funktionen einer exponentiellen Abnahme beschreiben lässt. Die toxikologische Untersuchung des mit UV-C und/oder nf-US behandelten Wassers mit dem Fischeitest und dem Leuchtbakterientest ergab keinen Hinweis auf die Bildung toxischer Nebenprodukte. Diese Studie zeigt, dass nf-US mit Dosen, die gegen eine Vielzahl an Parasiten wie Ciliaten, Nematoden und Crustaceen wirksam sind, sicher eingesetzt werden kann. Die Kombination von nf-US und UV-C könnte ein geeignetes Verfahren sein, um alle relevanten Pathogene in Kreislaufanlagen zu kontrollieren. / Recirculating aquaculture systems are well-known as environmentally friendly and high water-efficient production systems. The high stocking densities and low water exchange leads to an increased risk of infectious diseases. In this project the combination of low frequency ultrasound (LFUS) with ultraviolet-C (UV-C) light for the control of pathogens was studied. A comparative study about the efficiency of LFUS, UV-C and their combination against prokaryotic and eukaryotic model organisms was performed. Against bacteria, the application of UV-C was very effective, while the application of LFUS was not effective. However, a pretreatment of the water with LFUS decreased the average size of the suspended particles and improved the bactericidal effect of UV-C light. Compared to the low bactericidal dose of UV-C, a much higher UV-C dose was required for inactivation of eukaryotic model organisms. A significant increase of UV-C dose, however, can be limited by the possible photo-induced formation of nitrite from nitrate. Alternatively, LFUS can be used to kill eukaryotic parasites. However, the efficiency of LFUS differed greatly between species and can be well described by functions of an exponential decay. The evaluation of whole effluent toxicity by using the fish egg test and luminescent bacteria test revealed no evidence of toxic disinfection by-products formation during UV-C irradiation and/or LFUS sonication. This study shows that LFUS can be applied safely at energy densities that are effective against a wide range of eukaryotic parasites like ciliates, nematodes and crustaceans. The combination of LFUS and UV-C could provide an appropriate water treatment with respect to all relevant pathogens in recirculating aquaculture systems.

Det kommer ske en stor utbyggnad av elektrolys för produktion av vätgas i Sverigeoch övriga världen. För att sänka produktionskostnaden och därmed göra vätgasenbilligare analyserar denna rapport vilket behov av syrgas och värme som en landbaserad fiskodling har, samt hur mycket syrgas och värme fiskodlingen skulle behövaköpa från en elektrolysör. Analysen visar att de arter som används i studien - tilapia(Oreochromis, Oreochromis,. Alcolapia), regnbåge (Oncorhynchus mykiss) och lax(Salmo salar) - i en odling som producerar 40 ton fisk om året skulle förbruka cirka1,16 procent av syrgasen och cirka 0,35 procent av värmen från en 3 MW PEMelektrolysör. Försäljningsvärdet av syrgasen och värmen från en 3 MW elektrolysörberäknas till cirka 695 000 SEK/år för syrgasen och cirka 1 830 000 SEK/år för värmen. Den genomsnittliga kostnaden för syrgas och värme för arterna i studien i enodling på 40 ton/år beräknas till 8900 SEK/år för syrgasen och 6400 SEK/år förvärmen i en landbaserad fiskodling. / There will be a major expansion of electrolysis for production of hydrogen in Sweden and the rest of the world. To reduce production costs and thus make hydrogencheaper, this report analyzes how much oxygen and heat a fish farm consumes andtherefore would need to buy from an electrolyser. The analysis shows that the species used in the study - tilapia (Oreochromis, Oreochromis, Alcolapia), rainbow(Oncorhynchus mykiss) and salmon (Salmo salar) - in a farm that produces 40 tonsof fish per year would consume 1.16 percent of the oxygen and 0,35 percent of theheat produced from a 3 MW PEM electrolyzer. The value of the oxygen and theheat from a 3 MW electrolyser is calculated at SEK 694,939/year for the oxygenand SEK 1,829,813/year for the heat. The average cost for the species in the studyin a 40 tonne/year fish farm is calculated at SEK 8,900/year for the oxygen and SEK6,400/year for the heat in a land-based fish farm.