A Síndrome da Imunodeficiência Adquirida (AIDS) é uma doença de amplo espectro de manifestações, sendo razão de preocupação para qualquer autoridade sanitária. A terapêutica da AIDS é complexa sendo utilizados vários medicamentos, diversas vezes ao dia. Deste modo, objetivou-se o desenvolvimento de formas farmacêuticas sólidas como comprimidos tamponados mastigáveis (CTM), comprimidos com revestimento gastro-resistentes (CRGR) e pellets (PEL) para a veiculação de didanosina (ddl). Seis especialidades farmacêuticas na forma de CTM foram estudadas quanto ao perfil de dissolução, pH do meio e capacidade neutralizante ácida (CNA). Formulações teste de CTM foram propostas visando obter CNAs e perfis de dissolução adequados. Também foram testadas formulações de comprimidos e de pellets para posterior revestimento com filme gastro-resistente derivado do ácido metacrílico. Os ensaios de dissolução das amostras de CTM revelaram diferenças nas características de liberação do fármaco. Também foram observadas diferenças relacionadas a CNA. As formulações de CTM propostas apresentaram, na maioria dos casos, adequados perfis de dissolução e CNA. As formulações CRGR que receberam revestimento gastro-resistente apresentaram perfis de dissolução de ddl adequados, entretanto os comprimidos testados intumesceram em meio ácido, indicando descontinuidade do filme polimérico sobre os comprimidos. Testes para a produção de pellets veiculando ddl mostraram-se adequados quanto à morfologia e dissolução do fármaco, o mesmo sendo observado após o revestimento com filme gastro-resistente. / The Acquired Immune Deficiency Syndrome (AIDS) is a disease that manifests itself in a myriad of ways. Because of this, the condition has been subject of concern to all sanitary authorities. The treatment of AIDS is complex and many types of medicine are used, many times a day. The objective of the present study was to develop solid pharmaceutical dosage forms such as buffered chewable tablets (CTM), gastro-resistant coating tablets (CRGR) and pellets (PEL) for the loading of didanosine (ddl). Six pharmaceutical specialties in the form of CTM were studied so as to identify the profile of the dissolution, the pH of the environment, and the neutralizing acid capacity (CNA). The use of CTM tests formulations was proposed with the objective of obtaining adequate CNA and dissolution profiles. Different compositions of tablets and pellets were tested for a later addition of gastro-resistant film derived from the methacrylic acid. The experiments on the dissolution of the sample of CTM showed differences in the characteristic of the release of the substance. Differences related to the CNA were also observed. The formulations of the CTM proposed showed to have, in the most number of the cases, both adequate dissolution behavior and CNA. The formulations of the CRGR that had received the gastro-resistant coating showed adequate profile of ddl dissolution; the tested tablets, however, swelled in the acid environment, therefore indicating a lack of continuity of the polymeric film over the tablets. The tests for the production of pellets showed adequate results as to its morphology and dissolution of ddl. The same was observed after coating the pellets with gastro-resistant film.

Introdução: O Câncer de próstata metastático (mCaP) é uma doença incurável com progressão para o mCaP resistente à castração (mCPRC) após terapia de deprivação androgênica. Os microRNAs (miR) -23b e -27b tem ação antioncogênica e são suprimidos neste contexto. O gene da ciclina G1 (CCNG1) codifica uma quinase dependente de ciclina com potencial de inibição do crescimento e é um dos alvos dos miR-23b/-27b. Objetivos: Estimular os miR-23b/-27b isoladamente e em conjunto para avaliar e comparar o crescimento tumoral e a expressão do gene alvo CCNG1 em relação ao grupo controle em xenenxertos de PC-3M-luc-C6 em camundongos atímicos castrados. Métodos: Xenoenxertos subcutâneos da linhagem celular PC-3M-luc-C6 foram implantados em camundongos machos BALB/c nude. Os animais foram castrados 10 dias após o implante e utilizamos injeções intratumorais para induzir o aumento da expressão dos miR-23b/-27b separadamente e em conjunto através de Pre-miR® específicos. Realizamos avaliações semanais da bioluminescência (BLI) para avaliar o crescimento tumoral após a castração. Utilizamos a reação em cadeia de polimerase reversa em tempo real (qRT-PCR) para analisar a expressão da CCNG1 e os animais foram sacrificados 21 dias após a castração. Dividimos um total de 21 xenoenxertos nos seguintes grupos de tratamento: 4 no grupo controle, 5 no grupo Pró miR-23b, 6 no grupo Pró miR-27 e 6 no grupo Pró miR-23b associado ao Pró miR-27b. Resultados: Confirmamos o sucesso da transfecção dos miRs por qRT-PCR, e apresentamos o achado de superexpressão relativa da CCNG1 em relação ao grupo controle em: 9% (p=0,76), 46% (p=0,05) e 203% (p=0,01) nos grupos Pró miR-23b, Pró miR-27b e Pró miR-23b associado ao Pró miR-27b respectivamente. Comparamos o crescimento proporcional de cada tumor através da BLI, por meio da leitura no momento da castração ao final do experimento. Obtivemos um crescimento de 13,5; 8,69; 5,96 e 9,98 vezes nos grupos: controle, Pró miR-23b, Pró miR-27b e Pró miR-23b associado ao Pró miR-27b respectivamente. Conclusão: Demonstramos um modelo in vivo de CPRC que apresentou supreexpressão da CCNG1 após o tratamento intratumoral que aumentou a expressão dos miRs -23b e -27b. Este conjunto de miRs tem ação antioncogênica descrita no contexto do mCPRC e a sua estimulação neste contexto aumentou a expressão da CCNG1. Nosso estudo sugere que a CCNG1 deve apresentar uma ação pró-apoptótica quando superexpresso pelos miRs-23b/-27 no CPRC / Introduction: Metastatic prostate cancer (mPCa) is an incurable disease that invariably progresses to castration-resistant mPCa (mCRPC) after androgen deprivation therapy. The microRNAs miR-23b/-27b have been reported as tumor suppressors and are underexpressed in this context. The cyclin G1 gene (CCNG1) encodes a cyclin-dependent kinase with potential growth inhibitory activity that is a potential target of miR-23b/-27b. Objectives: We aim to explore a bioluminescent xenograft model of CRPC in castrated mice the effect positive modulation of the miR-23b/-27b on CCNG1 expression and mCRPC growth. Material and Methods: We injected subcutaneous xenografts of PC-3M-luc-C6 PCa cell line in BALB/c nude male mice. We neutered the animals after 10 days and used intratumoral injections up-regulating miR-23b/-27b separately and simultaneously through specific Pre-miRTM. We used weekly bioluminescence imaging (BLI) to assess tumor growth after castration and real-time polymerase chain reaction (qRT-PCR) to analyze the expression of CCNG1. We sacrificed the animals 21 days after castration. We randomized 21 xenografts in experimental groups as follows: n=4 in the negative control group; n=5 in Pro miR-23b group; n=6 in Pro miR-27b group and an n=6 tumors in the Pro miR-23b plus Pro miR-27b. Results: We confirmed successful transfection of both miRNAs with overexpression of CCNG1 of 9% (p=0.76), 46% (p=0.05) and 203% (p=0.01) in the Pro miR-23b, Pro miR-27b and Pro miR-23b plus -27b groups respectively. We compared the fold-change in BLI growth by the end of experiment finding an increase of 13.5-fold, 8.69-fold, 5.96-fold and 9.98-fold in groups Pro miR-negative control, Pro miR-23b, Pro miR-27b and Pro miR-23b plus Pro miR-27b groups respectively. Conclusions: We showed an in vivo model with overexpression of CCNG1 upon artificial upregulation of miR-23b and -27b in CRPC. This cluster of antineoplastic miRNA increased the expression of this cyclin, often described as oncogenic. Our study suggests that CCNG1 has a pro-apoptotic role when up-regulated by miR-23b/-27b in CPRC

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The present study reports a serologic survey of the main viral infections of dogs in Santa Maria, RS, Brazil, and the production of cell lines of canine, swine and leporine origin resistant to bovine viral diarrhea virus (BVDV). Canine distemper virus (CDV), parvovirus (CPV), adenovirus (CAV) and coronavirus (CCoV) infections have been associated with significant morbidity and mortality among dogs worldwide. The aim of this study was to determine the prevalence of antibodies against these viruses in the canine population of Santa Maria. To this purpose, 817 blood samples were collected from non-vaccinated dogs of 14 neighborhoods and tested by virus neutralization (CDV, CAV and CCoV) and by hemagglutining inhibition (CPV). Specific antibodies to CDV were detected in 27.3% (223/817) of the samples, to CPV in 68.7% (561/817), to CAV in 43% (353/817) and to CCoV in 50.4% (412/817) of the dogs. These results indicate that CDV, CPV, CAV and CCoV infections are spread among dogs in Santa Maria. However, a significant part of the population is seronegative and therefore unprotected against these viruses. This indicates a need for extending the vaccination programs against these viruses. During the standardization of serologic tests and expansion of cell cultures for virus amplification, the canine MDCK cell line was found to be contaminated with BVDV, the main viral contaminant of cultured cells. The inadverted contamination of cultured cells with BVDV may represent a serious problem for diagnostic virology, research and production of biologicals. The second part of this dissertation reports the production and characterization of three cell lines resistant BVDV, obtained out of each parental cell line (canine MDCK, porcine PK-15 and leporine RK-13) that were contaminated with BVDV. Initially, the cells were submitted to four rounds of infection with a highly cytolytic BVDV strain. The cells surviving infection were then cloned out, expanded and assayed for their susceptibility to BVDV. The resistance to BVDV was investigated by search for viral proteins by immunofluorescence and by cocultivation with susceptible cells following inoculation of BVDV at high titers. All three cell lines were resistant to three standard BVDV strains (Singer, NADL e Oregon) and 10 field isolates. Inoculation of these cells with BVDV at a multiplicity of infection of 10 TCID50/cell resulted in frequencies of infection of <10-5 for MDCK-R and PK-15R cells and of 3,3x10-4 for RK- 13R. Compared to the parental ones, the resistant cells were >10.000 (MDCK-R), >20.000 (PK-15R) and 600 (RK-13R) times less susceptible to BVDV. The inoculation of virus in the resistant cells in the presence of polyethylene-glicol (PEG) resulted in an increase in susceptibility in the order of >437 (MDCK-R), >346 (PK-15R) and 87 (RK-13R) times. These results indicate that the resistance of these cell lines is probably due to a block in viral entry which can be overcome by addition of PEG. On the other hand, each resistant cell line retained the susceptibility to other three viruses of interest which replicate in the parental cells. Thus, these cells may be useful for virology diagnostic, virus propagation and for vaccine production, without the risk of being inadvertedly contaminated with BVDV. / O presente trabalho relata um inquérito sorológico das principais infecções víricas de cães em Santa Maria, RS, Brasil e a obtenção de linhagens celulares de origem canina, suína e leporina resistentes ao vírus da Diarréia Viral Bovina (BVDV). As infecções pelo vírus da cinomose (CDV), parvovírus (CPV), adenovírus (CAV) e coronavírus (CCoV) são importantes causas de morbidade e de mortalidade em cães em todo o mundo. Com o objetivo de determinar a prevalência de anticorpos contra esses vírus na população canina da cidade de Santa Maria, coletou-se amostras de sangue de 817 cães não-vacinados, em 14 bairros. Estas foram testadas pela técnica de soroneutralização (CDV, CAV e CCoV) ou inibição da hemaglutinação (CPV). Anticorpos específicos contra o CDV foram detectados em 27,3% (223/817) das amostras, contra o CPV em 68,7% (561/817), contra o CAV em 43% (353/817) e contra o CCoV em 50,4% (412/817) dos cães. Esses resultados demonstram que esses vírus estão difundidos na população canina dos bairros da cidade. Por outro lado, demonstram também que uma parte considerável da população é soronegativa e, portanto está desprotegida contra esses agentes, indicando a necessidade de se ampliar os programas de vacinação para essas infecções. Durante a padronização das técnicas sorológicas e expansão dos cultivos celulares para amplificação dos vírus, detectou-se a contaminação da linhagem de células caninas MDCK com o BVDV, o principal vírus contaminante de cultivos celulares. A contaminação inadvertida de cultivos celulares com o BVDV pode representar um sério problema para o diagnóstico virológico, pesquisa e produção de imunobiológicos. A segunda parte dessa dissertação descreve a produção e caracterização de três linhagens celulares resistentes ao BVDV, obtidas a partir das células parentais de origem canina (MDCK), suína (PK-15) e leporina (RK-13) que estavam contaminadas com o BVDV. Essas células foram submetidas a quatro ciclos de infecção com uma cepa citolítica de BVDV. As células que sobreviveram a infecção lítica foram clonadas, expandidas e testadas para a sua susceptibilidade ao BVDV e outros vírus de interesse. A resistência ao BVDV foi investigada pela pesquisa de antígenos virais por imunofluorescência indireta e por cocultivo com células susceptíveis após a inoculação do vírus em altos títulos. As três linhagens celulares demonstraram ser resistentes a três cepas-padrão (Singer, NADL e Oregon) e a 10 isolados de campo do BVDV. A inoculação do BVDV nessas células com uma multiplicidade de infecção de 10 DICC50/célula resultou em freqüências de infecção de <10-5 para as células MDCK-R e PK-15R; e de 3,3x10-4 para as células RK-13R. Comparando-se com as células parentais, verificou-se que as linhagens resistentes são >10.000 (MDCK-R), >20.000 (PK-15R) e 600 (RK-13R) vezes menos susceptíveis ao BVDV. A inoculação do vírus nas células resistentes na presença de polietilenoglicol (PEG) resultou em um aumento na susceptibilidade dessas células na ordem de >437 (MDCK-R), >346 (PK-15R) e 87 vezes (RK-13R). Esses resultados indicam que a resistência dessas linhagens ao BVDV reside em um bloqueio na penetração do vírus, que pode ser parcialmente revertido pela adição do PEG. Por outro lado, cada linhagem resistente conservou a susceptibilidade a outros três vírus que replicam nas células parentais. Essas características tornam essas linhagens celulares potencialmente úteis para o diagnóstico, amplificação de vírus e produção de vacinas, sem o risco de contaminação acidental com o BVDV.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-10-14T12:30:34Z No. of bitstreams: 1 vanessadealmeidabelo.pdf: 412659 bytes, checksum: 2be0607436379c3a9ca0f2415972f9be (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2016-10-22T13:05:05Z (GMT) No. of bitstreams: 1 vanessadealmeidabelo.pdf: 412659 bytes, checksum: 2be0607436379c3a9ca0f2415972f9be (MD5) / Made available in DSpace on 2016-10-22T13:05:05Z (GMT). No. of bitstreams: 1 vanessadealmeidabelo.pdf: 412659 bytes, checksum: 2be0607436379c3a9ca0f2415972f9be (MD5) Previous issue date: 2008-02-15 / Nos países tropicais, as perdas causadas pela infestação de carrapatos em bovinos acarretam um grande impacto no sistema de produção animal. Recentes estudos têm mostrado a importância de fatores genéticos ligados a resistência a carrapato em Bos taurus indicus e Bos taurus taurus e que as citocinas têm um papel crítico na prevenção ou progressão de doenças. O objetivo desse trabalho foi avaliar os níveis de expressão dos genes IL-10 e IL-4 relacionados ao perfil imunológico Th2 associado à susceptibilidade ao carrapato e os genes IL-2 e IFN- relacionados ao perfil imunológico Th1 associado à resistência ao parasito. Além destes genes, analisou-se o perfil de expressão do gene TLR-2, importante no processo de reconhecimento de patógenos e os genes IL-8 e TNF-α importantes no processo inflamatório inicial. Seis animais mais resistentes e seis animais mais susceptíveis de uma população F2 de 332 animais, originária do cruzamento de animais F1(½ Holandês: ½ Gir), foram selecionados baseado na contagem de carrapatos e valor genético. Amostras de tecido foram coletadas de pele no 5° e 12° dias após a infestação para extração de RNA total. As PCRs em tempo real foram realizadas usando o gene GAPDH como controle endógeno. Os animais resistentes e susceptíveis apresentaram aumento de expressão do gene IL-10 no 5° (p<0,01) e 12 ° dias após a infestação (p<0,05). O gene IL-2, nos animais resistentes e susceptíveis, no 5° dia após a infestação não apresentou alteração da expressão sendo que 12° dia, em ambos os grupos de animais, este gene passou a ser mais expresso em relação ao animal controle sugerindo um perfil de resposta imunológica do tipo de Th2 nos animais resistentes e susceptíveis nos primeiros dias após a infestação. O gene IL-4 apresentou uma tendência ao aumento de expressão nos animais resistentes e susceptíveis em relação ao controle, sendo o perfil Th2 sugerido atribuído a IL-10 produzida por linfócitos T regulatórios (p>0,05). O gene TNF- apresentou aumento de expressão nos animais susceptíveis no 5° dia após a infestação com posterior diminuição no 12° dia após a infestação (p<0,05). Nos animais resistentes não foi observada alteração da expressão deste gene, isto sugere que ele possa estar mais atuante no início do processo inflamatório, logo após a fixação do carrapato. A mesma observação estende-se para o gene IL-8, em que não foi verificada alteração de expressão nos animais resistentes, embora nos animais susceptíveis este gene apresentou diminuição da expressão no 12° dia após a infestação (p<0,05). Quanto ao gene IFN-, não houve diferença de expressão entre os animais resistentes e susceptíveis, sendo que este gene parece não estar relacionado ao mecanismo de resistência. O gene TLR-2 apresentou diminuição da expressão em ambos os grupos de animais. Estes resultados sugerem que a resposta imune adquirida avaliada neste trabalho não apresenta papel preponderante no mecanismo de resistência e que resposta imune inata poderia está envolvida no mecanismo de resistência ao carrapato. Portanto, avaliação da resposta imunológica horas após a fixação do carrapato poderia nos fornecer resultados mais conclusivos. / In tropical countries losses caused by tick infestation in cattle lead to a major impact on animal production systems. Recent studies have shown the importance of genetic factors linked to tick resistance in Bos indicus and Bos taurus as well as the critical role in the prevention or progression of diseases mediated by cytokines. The aim of this work was to evaluate gene expression of IL-10 and IL-4 in relation to tick susceptibility associated with the Th2 profile and gene expression of IL-2 and IFN- in relation to tick resistance associated with the Th1 profile. In addition, the expression of TLR-2, important in the process the recognition of pathogens, and TNF-α and IL-8 genes, important in the initial inflammatory process, were evaluated. Six tick-resistant and six tick-susceptible animals from a F2 population of 332 animals, originated from the cross of F1 animals (½ Holstein: ½ Gir), were selected based on tick count and breeding value for tick resistance. Skin biopsies were collected in the 5th and 12th days after tick infestation. The GAPDH was used as endogenous control to normalize the amount of starting cDNA target in the real-time PCR assay. Both resistant and susceptible animals showed increased gene expression of IL-10 in the 5th and 12th days after infestation in relation to control animal (p<0.05). The IL-2 gene showed no change of expression in the 5th day after infestation for the resistant and susceptible animals. In the 12th post infestation, both resistant and susceptible animals showed increased gene expression in relation to control animal. These results suggest an enhancement of Th2 profile through the increase of IL-10 mRNA levels and a possible inhibition of the Th1 pattern in both groups (resistant and susceptible) starting 5 days after infestation and return to normal by day 12. Despite our results suggest the occurrence of the Th2 profile, the susceptible and resistant animals did not show variation on gene expression for IL-4 in relation to control animal. The susceptible animals showed increased expression of TNF-α in the 5th day after infestation. However, in the 12th day post infestation it was noted a decrease in the gene expression level. The resistant animals showed no change in the expression of this gene in relation to control animals suggesting that TNF-α could be more actively expressed in the early steps of the inflammatory process. Similarly, the resistant animals showed no variation in the expression of IL-8 while the susceptible animals showed increased expression in the 12th day post infestation. There were no differences of expression between resistant and susceptible animals in relation to IFN-γ what suggests that this gene might not be involved in the resistance mechanism. The TLR-2 gene showed decreased expression in both resistant and susceptible animals (p<0.05). Finally, there was no difference in expression between susceptible and resistant animals in relation to all selected genes in the 5th and 12th days after infestation. These results suggest that the acquired immunity evaluated in this work might not have preponderant role in the resistance mechanism. The innate immunity might be playing a major role in the bovine tick resistance/susceptibility mechanism in early hours after infestation.

Algunas especies vegetales se consideran malezas debido a que alcanzan niveles poblacionales tales, que al competir con los cultivos causan perjuicios sobre su producción. Por este motivo, en determinadas ocasiones es necesario realizar operaciones de control sobre las poblaciones de malezas para reducir su infestación hasta valores que permitan obtener niveles de producción deseados. A nivel mundial el control químico ha sido el método más práctico y eficiente para controlar malezas, especialmente en cultivos extensivos. Sin embargo, el uso continuo de herbicidas desde hace más de 40 años trajo aparejado un impacto negativo sobre el medio ambiente, la salud y la proliferación de malezas resistentes. Estas consecuencias se deben tener en cuenta para definir manejos adecuados a largo plazo. Desde una perspectiva estratégica, un programa sostenible de manejo de malezas debería basarse en una combinación de métodos tanto preventivos como curativos que apliquen principios basados en el conocimiento, dando lugar a lo que se conoce como Manejo Integrado de Malezas, MIM. En este contexto, el modelado matemático se presenta como una herramienta apropiada para ayudar a guiar el proceso de toma de decisiones asociado al MIM. En esta tesis se propone un modelo de simulación para asistir en la toma de decisiones relacionadas con el MIM. El modelo se desarrolló en colaboración con profesionales extensionistas y fue concebido como una herramienta flexible y adaptable a diversos sistemas de producción agrícola, poseyendo un mayor nivel de detalle que otros modelos similares. Dicho modelo permite simular la dinámica multianual de una maleza en competencia con el cultivo. El ciclo de vida de la maleza se representa a través de los componentes demográficos típicos (ej. banco de semillas, plántulas, adultos en estado vegetativo y reproductivo, producción de semillas). El desarrollo del cultivo se simula de forma simplificada a fin de cuantificar diariamente los efectos de la competencia interespecífica. La simulación de distintas estrategias de MIM permite calcular y comparar indicadores económicos, ambientales y agronómicos. Palabras claves: Manejo Integrado de Malezas, Modelado de sistemas agrícolas, Avena fatua L., Euphorbia davidii Subils., Trigo, Cebada, Soja, Competencia de malezas, Asistencia a la toma de decisiones, Margen bruto, Valor actual, Impacto ambiental, Malezas resistentes, Dinámica poblacional de malezas. / Some species considered weeds reach population levels that impact on crops’ production. For this reason, on certain occasions, it is necessary to carry out control operations to reduce weed infestations to levels compatible with reasonable crops’ yields. Worldwide, chemical control has been the most practical and efficient method for controlling weeds, especially in extensive crops. However, the continuous use of herbicides for over 40 years generated a well-known negative impact on environment and health and the proliferation of resistant species. These environmental consequences must be taken into account to define appropriate long-term management practices. From a strategic perspective, a sustainable weed management program should be based on a combination of preventive and curative methods that apply knowledge-based principles, leading to as the so-called Integrated Weed Management (IWM). In this context, mathematical modelling arises as an appropriate tool to assist in the decision- making process associated with IWM. This thesis proposes a simulation model to support decision-making related to IWM. The model was developed in collaboration with extensionists and was conceived as a flexible and adaptable tool for various agricultural production systems, possessing a higher level of detail than similar models. This model simulates multi-year dynamics of a weed in competition with a crop. The weed's life cycle is represented through typical demographic components (e.g. seed bank, seedlings, vegetative and reproductive individuals, and seed production). The development of the crop is simulated in a simplified manner in order to quantify the daily effects of interspecific competition. The simulation of different IWM strategies allows for the quantification and comparison of economic, environmental, and agronomic indexes.

Este trabalho traz contribuições à linha de pesquisa \'Nova Família de Ligas Baseada no Sistema Ni-Al-Cr-C Resistentes ao Desgaste em Elevadas Temperaturas\', estudando o comportamento mecânico, tribológico e a estabilidade superficial destas ligas. Esta família de ligas fundidas, denominada NICRALC - busca conjugar algumas características das superligas à base de níquel e dos ferros fundidos brancos, aliando ao comportamento mecânico anômalo do Ni3Al - aumento da resistência mecânica com a temperatura, até valores da ordem de 800°C - a uma dispersão de carbonetos de cromo de alta dureza. Desta forma, busca-se desenvolver uma alternativa às ligas ferrosas nas temperaturas acima das quais estas ligas perdem significativamente sua resistência mecânica (notadamente 600 °C), assim como uma alternativa mais econômica às ligas a base de cobalto resistentes ao desgaste, em virtude das altas cotações e instabilidades no preço deste elemento. Neste trabalho foram avaliadas as propriedades mecânicas em elevadas temperaturas (compressão e dureza), tenacidade à fratura na temperatura ambiente, comportamento tribológico (cavitação, abrasão, deslizamento e erosão) e a resistência à oxidação ao ar e à carburação em atmosfera redutora, em temperaturas elevadas, de ligas Ni-Al-Cr-C (NICRALC) fundidas. Ligas NICRALC fundidas com diferentes teores de carbono e submetidas a diferentes tratamentos tiveram seu comportamento mecânico e tribológico comparado ao comportamento do STELLITE 6 fundido, a uma liga NICRALC produzida por deposição por \'SPRAY\', a um ferro fundido branco alto cromo fundido convencionalmente e a um ferro fundido branco alto cromo depositado por \'SPRAY\'. Os ensaios de compressão confirmaram o comportamento anômalo da matriz ordenada Ni3Al. As ligas NICRALC apresentaram aumento ou manutenção da resistência ao escoamento com o aumento da temperatura de ensaio. A mesma tendência foi encontrada nos ensaios de dureza a quente. As demais ligas apresentaram tendência de queda da resistência ao escoamento e da dureza com o aumento da temperatura de ensaio. Esta tendência é mais acentuada na liga STELLITE, desta forma, as ligas NICRALC apresentaram em altas temperaturas maior resistência ao escoamento e maior dureza que o STELLITE. Os diferentes ensaios de desgaste mostraram, em geral, uma maior resistência do STELLITE em comparação com as ligas NICRALC na temperatura ambiente. Nestas condições verifica-se que a menor resistência mecânica da matriz ordenada nas ligas NICRALC é determinante para definir uma menor resistência ao desgaste na temperatura ambiente. Nos ensaios de erosão-oxidativa realizados a 600 e 800°C as ligas NICRALC mostraram perdas de massa menores que as experimentadas pelo STELLITE e pelos ferros fundidos. As ligas NICRALC com microestrutura de carbonetos mais homogênea, próxima do eutético, mostraram melhor comportamento sob desgaste abrasivo e erosivo se comparadas às ligas NICRALC 05 e 13. Os ensaios de resistência à oxidação mostraram que as ligas NICRALC são mais resistentes à oxidação que o STELLITE. Além disso, observou-se grande propensão ao destacamento dos filmes de óxidos formados nos STELLITES em temperaturas acima de 800°C. Os ensaios de resistência à carburação mostraram que no caso das ligas NICRALC ocorreu a precipitação de um depósito de grafita e, subjacente a esta, a formação de uma zona de fragmentação microestrutural, na qual se observa empobrecimento ora do Al ora do Cr, que aparentemente impede o avanço do processo. No caso do STELLITE verificou-se a ocorrência do aumento da fração volumétrica de carbonetos, típico dos casos clássicos de carburação. Os procedimentos de simulação termodinâmica utilizados indicam a necessidade de se implementar a descrição da solubilidade do carbono na fase ordenada de forma a permitir o pleno uso do software THERMOCALC no projeto e aprimoramento de ligas NICRALC. / This work contributes to the research line \'New Family of High Temperature Wear Resistant Alloys based on the Ni-Al-Cr-C System\', studying the mechanical and tribological behaviour and the surface stability of these alloys. This family of foundry alloys, called NICRALC, tries to unite some of the characteristics of the Ni based superalloys and the high-chromium-white-cast-irons, associating the anomalous behaviour of the ordered intermetallic phase Ni3Al - which increases its strength with the increase of temperature - with a dispersion of hard chromium carbides. The aim is to develop an alternative to iron-based wear resistant alloys at temperatures where they loose significantly their strength, as well as substituting cobalt based high temperature wear resistant alloys, which suffer from the instability and high cost of the Co metal. The high temperature mechanical properties (hardness and compression), room temperature fracture toughness, tribological behaviour (cavitation, abrasion, sliding and erosion) and resistance to high temperature oxidation and carburization of cast Ni-Al-Cr-C (NICRALC) alloys are studied. Cast NICRALC alloys with different C contents and different heat treatments were compared with a cast STELLITE 6, a conventionally cast high chromium white cast iron, a spray formed high chromium white cast iron and a spray formed NICRALC. Mechanical tests in compression confirmed that NICRALC alloys share the anomalous behaviour of the ordered intermetallic phase Ni3Al, increasing or maintaining their yield strength with increased testing temperature. The same occurred with hot hardness tests. STELLITE and all other alloys showed loss of strength with increased testing temperatures. Thus NICRALC alloys showed higher strength and hardness than STELLITE at high temperatures. All wear tests showed a higher wear resistance of STELLITE in comparison with NICRALC at room temperature. This result can be explained by the higher matrix hardness of STELLITE at room temperature. Oxidative-erosion tests run at 600 and 800° C showed that NICRALC suffered smaller mass loss than STELLITE and the white cast irons. NICRALC alloys with more homogeneous carbide distributions (eutectic alloys) obtained the best results under severe oxidation-erosion conditions. NICRALC alloys were more oxidation resistant than STELLITE alloy, which tended to break and detach the oxide layer formed above 800°C under air. During carburization essays in a reducing atmosphere the NICRALC alloys tended to form a coke-like graphite layer, over a layer with a fragmented microstructure depleted alternatively in chromium and aluminum, which effectively stopped the advance of the process. The STELLITE alloy suffered an increase in carbide volume fraction, a classic carburizing behaviour. The thermodynamic simulation results show that it is still needed to introduce the solubility of carbon on the ordered Ni3Al phase in the model in order to be able to fully calculate the NICRALC phase diagrams.

Made available in DSpace on 2014-07-29T15:26:22Z (GMT). No. of bitstreams: 1 TeseMariaAparecidaSVieira2010.pdf: 800058 bytes, checksum: 8dafcb160a1972a06f2d836a5f52d813 (MD5) Previous issue date: 2010-07-05 / Nasal carriage of Staphylococcus aureus methicillin-resistant (MRSA) is known to be a risk for subsequent infection. The MRSA carriers are an emergent and hidden reservoir in community and in the health-care environment. The aim of this investigation were to assess the prevalence and risk factors for MRSA nasal carriage in children attending emergency departments (ED) and Neonatal Intensive Care Units (NICU), and to describe the molecular features of such isolates. Methods: Nasal swabs were obtained from children less than 60 months of age attending ED, and from newborns of the four NICUs of Goiânia city, central Brazil, in 2007 and 2008. The definition of MRSA followed the CLSI criteria. Exposure variables to S. aureus and MRSA carriers were gathered through in-person interviews with mothers and hospital records. Univariate and multivariate logistic regression were performed to identify risk factors for S. aureus and MRSA carriage. Molecular typing was evaluated by pulsed field gel electrophoresis, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence type (MLST). Results: A total of 2,735 children were enrolled. At the ED (n=2.034), the prevalence respectively of nasal carriages for S. aureus and MRSA were 20% (n=408) and 0.2% (n=4). Among NICUs (total of infants = 701), the prevalence of nasal carriage ranged from 0.03% to 15.7% for S. aureus and, from 0.0% to 2.0% for MRSA. At the ED, MRSA carriage was independently associated with child-care attendance in the previous 6 months (OR=10.6; p=0.045) and congenital malformation (OR=26.8; p=0.002). All nasal carriers at NICUs were from private hospitals. Only length of hospitalization was associated with MRSA nasal carriage at NICUs (p=0.023). Among four MRSA nasal carrier at ED, one harbored SCCmec type III, and three SCCmec type IV. Among four children from at the NICUs two infants harbored SCCmec type III, and two SCCmec type IV. All MRSASCCmec type III were multidrug-resistants. Strains related to Pediatric/USA800 and Brazilian MRSA clones were detected in both, ED and NICUs. One MRSA cluster related to Western Australia/USA400 was detected in ED. Conclusions: Children visiting ED, especially those reporting day-care attendance, and neonates from NICUs may play a role in spreading MRSA in healthcare settings. The study suggests cross transmission of MRSA type III and type IV between ED and hospital environments. / Portador nasal de Methicillin-resistant Staphylococcus aureus (MRSA) é um preditor de infecção subseqüente. Portadores de MRSA são um reservatório oculto, emergente na comunidade e nos serviços de saúde. Os objetivos deste estudo foram avaliar a prevalência e fatores de risco do portador nasal por S. aureus e MRSA em crianças atendidas em Pronto Atendimento (PA) e admitidas em Unidades de Cuidado Intensivo Neonatal (UCINs) e descrever as características moleculares de tais isolados. Método: Swabs nasais foram obtidos de crianças menores de 60 meses atendidas em PA e de neonatos de quatro UCINs do município de Goiânia, Brasil, em 2007 e 2008. A definição de MRSA seguiu critérios definidos pelo CLSI. Variáveis de exposição para portadores de S. aureus e MRSA foram obtidas por meio de entrevistas com mães e registros hospitalares. Regressão logística multivariada foram realizadas para identificar fatores de risco. A tipagem molecular foi feita por meio de staphylococcal cassette chromosome mec (SCCmec) typing, Pulsed Field Gel Electrophoresis (PFGE) e seqüenciamento de multilocus enzimáticos (MLST). Resultados: Um total de 2.735 crianças foi recrutado. No PA (n=2.034), as prevalências de portador nasal para S. aureus e MRSA foram de 20,1% (n=408) e de 0,2% (n=4), respectivamente. Nas UCINs (n= 701), a prevalência de portador nasal variou de 0,03% a 15,7% para S. aureus (n=64) e, de 0,0% a 2,0% para MRSA (n=4). No PA, o portador de MRSA foi independentemente associado à frequência de creche nos últimos 6 meses (OR=10,6; p=0,045) e malformação congênita (OR=26,8; p=0,002). Todos os portadores nasais de MRSA nas UCINs eram crianças internadas em hospitais privados e a única variável associada ao portador MRSA foi tempo de internação (p=0,023). Das quatro crianças portadores de MRSA no PA, uma portava SCCmec tipo III e, três, SCCmec tipo IV. Nas UCINs, duas crianças eram SCCmec tipo III e duas, SCCmec tipo IV. Todas as cepas SCCmec tipo III eram multidrogarresistentes (CLSI). Cepas MRSA relacionadas ao clones pediátrico/USA800 e epidêmico brasileiro foram detectados no PA e nas UCINs. Um cluster MRSA relacionado ao clone Western Australia/WA-1/ USA400 foi encontrado no PA. Conclusão: Crianças atendidas no pronto atendimento, especialmente aquelas que freqüentaram creche, e neonatos de UCINs apresentam potencial para disseminação de MRSA nos serviços de saúde. O estudo sugere transmissão cruzada de MRSA SCCmec tipo III e tipo IV entre serviços de emergência e hospitais.

Este trabalho traz contribuições à linha de pesquisa \'Nova Família de Ligas Baseada no Sistema Ni-Al-Cr-C Resistentes ao Desgaste em Elevadas Temperaturas\', estudando o comportamento mecânico, tribológico e a estabilidade superficial destas ligas. Esta família de ligas fundidas, denominada NICRALC - busca conjugar algumas características das superligas à base de níquel e dos ferros fundidos brancos, aliando ao comportamento mecânico anômalo do Ni3Al - aumento da resistência mecânica com a temperatura, até valores da ordem de 800°C - a uma dispersão de carbonetos de cromo de alta dureza. Desta forma, busca-se desenvolver uma alternativa às ligas ferrosas nas temperaturas acima das quais estas ligas perdem significativamente sua resistência mecânica (notadamente 600 °C), assim como uma alternativa mais econômica às ligas a base de cobalto resistentes ao desgaste, em virtude das altas cotações e instabilidades no preço deste elemento. Neste trabalho foram avaliadas as propriedades mecânicas em elevadas temperaturas (compressão e dureza), tenacidade à fratura na temperatura ambiente, comportamento tribológico (cavitação, abrasão, deslizamento e erosão) e a resistência à oxidação ao ar e à carburação em atmosfera redutora, em temperaturas elevadas, de ligas Ni-Al-Cr-C (NICRALC) fundidas. Ligas NICRALC fundidas com diferentes teores de carbono e submetidas a diferentes tratamentos tiveram seu comportamento mecânico e tribológico comparado ao comportamento do STELLITE 6 fundido, a uma liga NICRALC produzida por deposição por \'SPRAY\', a um ferro fundido branco alto cromo fundido convencionalmente e a um ferro fundido branco alto cromo depositado por \'SPRAY\'. Os ensaios de compressão confirmaram o comportamento anômalo da matriz ordenada Ni3Al. As ligas NICRALC apresentaram aumento ou manutenção da resistência ao escoamento com o aumento da temperatura de ensaio. A mesma tendência foi encontrada nos ensaios de dureza a quente. As demais ligas apresentaram tendência de queda da resistência ao escoamento e da dureza com o aumento da temperatura de ensaio. Esta tendência é mais acentuada na liga STELLITE, desta forma, as ligas NICRALC apresentaram em altas temperaturas maior resistência ao escoamento e maior dureza que o STELLITE. Os diferentes ensaios de desgaste mostraram, em geral, uma maior resistência do STELLITE em comparação com as ligas NICRALC na temperatura ambiente. Nestas condições verifica-se que a menor resistência mecânica da matriz ordenada nas ligas NICRALC é determinante para definir uma menor resistência ao desgaste na temperatura ambiente. Nos ensaios de erosão-oxidativa realizados a 600 e 800°C as ligas NICRALC mostraram perdas de massa menores que as experimentadas pelo STELLITE e pelos ferros fundidos. As ligas NICRALC com microestrutura de carbonetos mais homogênea, próxima do eutético, mostraram melhor comportamento sob desgaste abrasivo e erosivo se comparadas às ligas NICRALC 05 e 13. Os ensaios de resistência à oxidação mostraram que as ligas NICRALC são mais resistentes à oxidação que o STELLITE. Além disso, observou-se grande propensão ao destacamento dos filmes de óxidos formados nos STELLITES em temperaturas acima de 800°C. Os ensaios de resistência à carburação mostraram que no caso das ligas NICRALC ocorreu a precipitação de um depósito de grafita e, subjacente a esta, a formação de uma zona de fragmentação microestrutural, na qual se observa empobrecimento ora do Al ora do Cr, que aparentemente impede o avanço do processo. No caso do STELLITE verificou-se a ocorrência do aumento da fração volumétrica de carbonetos, típico dos casos clássicos de carburação. Os procedimentos de simulação termodinâmica utilizados indicam a necessidade de se implementar a descrição da solubilidade do carbono na fase ordenada de forma a permitir o pleno uso do software THERMOCALC no projeto e aprimoramento de ligas NICRALC. / This work contributes to the research line \'New Family of High Temperature Wear Resistant Alloys based on the Ni-Al-Cr-C System\', studying the mechanical and tribological behaviour and the surface stability of these alloys. This family of foundry alloys, called NICRALC, tries to unite some of the characteristics of the Ni based superalloys and the high-chromium-white-cast-irons, associating the anomalous behaviour of the ordered intermetallic phase Ni3Al - which increases its strength with the increase of temperature - with a dispersion of hard chromium carbides. The aim is to develop an alternative to iron-based wear resistant alloys at temperatures where they loose significantly their strength, as well as substituting cobalt based high temperature wear resistant alloys, which suffer from the instability and high cost of the Co metal. The high temperature mechanical properties (hardness and compression), room temperature fracture toughness, tribological behaviour (cavitation, abrasion, sliding and erosion) and resistance to high temperature oxidation and carburization of cast Ni-Al-Cr-C (NICRALC) alloys are studied. Cast NICRALC alloys with different C contents and different heat treatments were compared with a cast STELLITE 6, a conventionally cast high chromium white cast iron, a spray formed high chromium white cast iron and a spray formed NICRALC. Mechanical tests in compression confirmed that NICRALC alloys share the anomalous behaviour of the ordered intermetallic phase Ni3Al, increasing or maintaining their yield strength with increased testing temperature. The same occurred with hot hardness tests. STELLITE and all other alloys showed loss of strength with increased testing temperatures. Thus NICRALC alloys showed higher strength and hardness than STELLITE at high temperatures. All wear tests showed a higher wear resistance of STELLITE in comparison with NICRALC at room temperature. This result can be explained by the higher matrix hardness of STELLITE at room temperature. Oxidative-erosion tests run at 600 and 800° C showed that NICRALC suffered smaller mass loss than STELLITE and the white cast irons. NICRALC alloys with more homogeneous carbide distributions (eutectic alloys) obtained the best results under severe oxidation-erosion conditions. NICRALC alloys were more oxidation resistant than STELLITE alloy, which tended to break and detach the oxide layer formed above 800°C under air. During carburization essays in a reducing atmosphere the NICRALC alloys tended to form a coke-like graphite layer, over a layer with a fragmented microstructure depleted alternatively in chromium and aluminum, which effectively stopped the advance of the process. The STELLITE alloy suffered an increase in carbide volume fraction, a classic carburizing behaviour. The thermodynamic simulation results show that it is still needed to introduce the solubility of carbon on the ordered Ni3Al phase in the model in order to be able to fully calculate the NICRALC phase diagrams.

In the present work, the load-bearing behaviour of Textile Reinforced Concrete (TRC), which is a composite of a fine-grained concrete matrix and a reinforcement of high-performance fibres processed to textiles, exposed to uniaxial tensile loading was investigated based on numerical simulations. The investigations are focussed on reinforcement of multi-filament yarns of alkali-resistant glass. When embedded in concrete, these yarns are not entirely penetrated with cementitious matrix, which leads associated with the heterogeneity of the concrete and the yarns to a complex load-bearing and failure behaviour of the composite. The main objective of the work was the theoretical investigation of effects in the load-bearing behaviour of TRC, which cannot be explained solely by available experimental results. Therefore, a model was developed, which can describe the tensile behaviour of TRC in different experimental test setups with a unified approach. Neglecting effects resulting from Poisson’s effect, a one-dimensional model implemented within the framework of the Finite Element Method was established. Nevertheless, the model takes also transverse effects into account by a subdivision of the reinforcement yarns into so-called segments. The model incorporates two types of finite elements: bar and bond elements. In longitudinal direction, the bar elements are arranged in series to represent the load-bearing behaviour of matrix or reinforcement. In transverse direction these bar element chains are connected with bond elements. The model gains most of its complexity from non-linearities arising from the constitutive relations, e. g., limited tensile strength of concrete and reinforcement, tension softening of the concrete, waviness of the reinforcement and non-linear bond laws. Besides a deterministic description of the material behaviour, also a stochastic formulation based on a random field approach was introduced in the model. The model has a number of advantageous features, which are provided in this combination only in a few of the existing models concerning TRC. It provides stress distributions in the reinforcement and the concrete as well as properties of concrete crack development like crack spacing and crack widths, which are in some of the existing models input parameters and not a result of the simulations. Moreover, the successive failure of the reinforcement can be studied with the model. The model was applied to three types of tests, the filament pull-out test, the yarn pull-out test and tensile tests with multiple concrete cracking. The results of the simulations regarding the filament pull-out tests showed good correspondence with experimental data. Parametric studies were performed to investigate the influence of geometrical properties in these tests like embedding and free lengths of the filament as well as bond properties between filament and matrix. The presented results of simulations of yarn pull-out tests demonstrated the applicability of the model to this type of test. It has been shown that a relatively fine subdivision of the reinforcement is necessary to represent the successive failure of the reinforcement yarns appropriately. The presented results showed that the model can provide the distribution of failure positions in the reinforcement and the degradation development of yarns during loading. One of the main objectives of the work was to investigate effects concerning the tensile material behaviour of TRC, which could not be explained, hitherto, based solely on experimental results. Hence, a large number of parametric studies was performed concerning tensile tests with multiple concrete cracking, which reflect the tensile behaviour of TRC as occurring in practice. The results of the simulations showed that the model is able to reproduce the typical tripartite stress-strain response of TRC consisting of the uncracked state, the state of multiple matrix cracking and the post-cracking state as known from experimental investigations. The best agreement between simulated and experimental results was achieved considering scatter in the material properties of concrete as well as concrete tension softening and reinforcement waviness. / Die vorliegende Arbeit beschäftigt sich mit Untersuchungen zum einaxialen Zugtragverhalten von Textilbeton. Textilbeton ist ein Verbundwerkstoff bestehend aus einer Matrix aus Feinbeton und einer Bewehrung aus Multifilamentgarnen aus Hochleistungsfasern, welche zu textilen Strukturen verarbeitet sind. Die Untersuchungen konzentrieren sich auf Bewehrungen aus alkali-resistentem Glas. Das Tragverhalten des Verbundwerkstoffs ist komplex, was aus der Heterogenität der Matrix und der Garne sowie der unvollständigen Durchdringung der Garne mit Matrix resultiert. Das Hauptziel der Arbeit ist die theoretische Untersuchung von Effekten und Mechanismen innerhalb des Lastabtragverhaltens von Textilbeton, welche nicht vollständig anhand verfügbarer experimenteller Ergebnisse erklärt werden können. Das entsprechende Modell zur Beschreibung des Zugtragverhaltens von Textilbeton soll verschiedene experimentelle Versuchstypen mit einem einheitlichen Modell abbilden können. Unter Vernachlässigung von Querdehneffekten wurde ein eindimensionales Modell entwickelt und im Rahmen der Finite-Elemente-Methode numerisch implementiert. Es werden jedoch auch Lastabtragmechanismen in Querrichtung durch eine Unterteilung der Bewehrungsgarne in sogenannte Segmente berücksichtigt. Das Modell enthält zwei Typen von finiten Elementen: Stabelemente und Verbundelemente. In Längsrichtung werden Stabelemente kettenförmig angeordnet, um das Tragverhalten von Matrix und Bewehrung abzubilden. In Querrichtung sind die Stabelementketten mit Verbundelementen gekoppelt. Das Modell erhält seine Komplexität hauptsächlich aus Nichtlinearitäten in der Materialbeschreibung, z.B. durch begrenzte Zugfestigkeiten von Matrix und Bewehrung, Zugentfestigung der Matrix, Welligkeit der Bewehrung und nichtlineare Verbundgesetze. Neben einer deterministischen Beschreibung des Materialverhaltens beinhaltet das Modell auch eine stochastische Beschreibung auf Grundlage eines Zufallsfeldansatzes. Mit dem Modell können Spannungsverteilungen im Verbundwerkstoff und Eigenschaften der Betonrissentwicklung, z.B. in Form von Rissbreiten und Rissabständen untersucht werden, was in dieser Kombination nur mit wenigen der existierenden Modelle für Textilbeton möglich ist. In vielen der vorhandenen Modelle sind diese Eigenschaften Eingangsgrößen für die Berechnungen und keine Ergebnisse. Darüber hinaus kann anhand des Modells auch das sukzessive Versagen der Bewehrungsgarne studiert werden. Das Modell wurde auf drei verschiedene Versuchstypen angewendet: den Filamentauszugversuch, den Garnauszugversuch und Dehnkörperversuche. Die Berechnungsergebnisse zu den Filamentauszugversuchen zeigten eine gute Übereinstimmung mit experimentellen Resultaten. Zudem wurden Parameterstudien durchgeführt, um Einflüsse aus Geometrieeigenschaften wie der eingebetteten und freien Filamentlänge sowie Materialeigenschaften wie dem Verbund zwischen Matrix und Filament zu untersuchen. Die Berechnungsergebnisse zum Garnauszugversuch demonstrierten die Anwendbarkeit des Modells auf diesen Versuchstyp. Es wurde gezeigt, dass für eine realitätsnahe Abbildung des Versagensverhaltens der Bewehrungsgarne eine relativ feine Auflösung der Bewehrung notwendig ist. Die Berechnungen lieferten die Verteilung von Versagenspositionen in der Bewehrung und die Entwicklung der Degradation der Garne im Belastungsverlauf. Ein Hauptziel der Arbeit war die Untersuchung von Effekten im Zugtragverhalten von Textilbeton, die bisher nicht durch experimentelle Untersuchungen erklärt werden konnten. Daher wurde eine Vielzahl von Parameterstudien zu Dehnkörpern mit mehrfacher Matrixrissbildung, welche das Zugtragverhalten von Textilbeton ähnlich praktischen Anwendungen abbilden, durchgeführt. Die Berechnungsergebnisse zeigten, dass der experimentell beobachtete dreigeteilte Verlauf der Spannungs-Dehnungs-Beziehung von Textilbeton bestehend aus dem ungerissenen Zustand, dem Zustand der Matrixrissbildung und dem Zustand der abgeschlossenen Rissbildung vom Modell wiedergegeben wird. Die beste Übereinstimmung zwischen berechneten und experimentellen Ergebnissen ergab sich unter Einbeziehung von Streuungen in den Materialeigenschaften der Matrix, der Zugentfestigung der Matrix und der Welligkeit der Bewehrung.

In the present work, the load-bearing behaviour of Textile Reinforced Concrete (TRC), which is a composite of a fine-grained concrete matrix and a reinforcement of high-performance fibres processed to textiles, exposed to uniaxial tensile loading was investigated based on numerical simulations. The investigations are focussed on reinforcement of multi-filament yarns of alkali-resistant glass. When embedded in concrete, these yarns are not entirely penetrated with cementitious matrix, which leads associated with the heterogeneity of the concrete and the yarns to a complex load-bearing and failure behaviour of the composite. The main objective of the work was the theoretical investigation of effects in the load-bearing behaviour of TRC, which cannot be explained solely by available experimental results. Therefore, a model was developed, which can describe the tensile behaviour of TRC in different experimental test setups with a unified approach. Neglecting effects resulting from Poisson’s effect, a one-dimensional model implemented within the framework of the Finite Element Method was established. Nevertheless, the model takes also transverse effects into account by a subdivision of the reinforcement yarns into so-called segments. The model incorporates two types of finite elements: bar and bond elements. In longitudinal direction, the bar elements are arranged in series to represent the load-bearing behaviour of matrix or reinforcement. In transverse direction these bar element chains are connected with bond elements. The model gains most of its complexity from non-linearities arising from the constitutive relations, e. g., limited tensile strength of concrete and reinforcement, tension softening of the concrete, waviness of the reinforcement and non-linear bond laws. Besides a deterministic description of the material behaviour, also a stochastic formulation based on a random field approach was introduced in the model. The model has a number of advantageous features, which are provided in this combination only in a few of the existing models concerning TRC. It provides stress distributions in the reinforcement and the concrete as well as properties of concrete crack development like crack spacing and crack widths, which are in some of the existing models input parameters and not a result of the simulations. Moreover, the successive failure of the reinforcement can be studied with the model. The model was applied to three types of tests, the filament pull-out test, the yarn pull-out test and tensile tests with multiple concrete cracking. The results of the simulations regarding the filament pull-out tests showed good correspondence with experimental data. Parametric studies were performed to investigate the influence of geometrical properties in these tests like embedding and free lengths of the filament as well as bond properties between filament and matrix. The presented results of simulations of yarn pull-out tests demonstrated the applicability of the model to this type of test. It has been shown that a relatively fine subdivision of the reinforcement is necessary to represent the successive failure of the reinforcement yarns appropriately. The presented results showed that the model can provide the distribution of failure positions in the reinforcement and the degradation development of yarns during loading. One of the main objectives of the work was to investigate effects concerning the tensile material behaviour of TRC, which could not be explained, hitherto, based solely on experimental results. Hence, a large number of parametric studies was performed concerning tensile tests with multiple concrete cracking, which reflect the tensile behaviour of TRC as occurring in practice. The results of the simulations showed that the model is able to reproduce the typical tripartite stress-strain response of TRC consisting of the uncracked state, the state of multiple matrix cracking and the post-cracking state as known from experimental investigations. The best agreement between simulated and experimental results was achieved considering scatter in the material properties of concrete as well as concrete tension softening and reinforcement waviness. / Die vorliegende Arbeit beschäftigt sich mit Untersuchungen zum einaxialen Zugtragverhalten von Textilbeton. Textilbeton ist ein Verbundwerkstoff bestehend aus einer Matrix aus Feinbeton und einer Bewehrung aus Multifilamentgarnen aus Hochleistungsfasern, welche zu textilen Strukturen verarbeitet sind. Die Untersuchungen konzentrieren sich auf Bewehrungen aus alkali-resistentem Glas. Das Tragverhalten des Verbundwerkstoffs ist komplex, was aus der Heterogenität der Matrix und der Garne sowie der unvollständigen Durchdringung der Garne mit Matrix resultiert. Das Hauptziel der Arbeit ist die theoretische Untersuchung von Effekten und Mechanismen innerhalb des Lastabtragverhaltens von Textilbeton, welche nicht vollständig anhand verfügbarer experimenteller Ergebnisse erklärt werden können. Das entsprechende Modell zur Beschreibung des Zugtragverhaltens von Textilbeton soll verschiedene experimentelle Versuchstypen mit einem einheitlichen Modell abbilden können. Unter Vernachlässigung von Querdehneffekten wurde ein eindimensionales Modell entwickelt und im Rahmen der Finite-Elemente-Methode numerisch implementiert. Es werden jedoch auch Lastabtragmechanismen in Querrichtung durch eine Unterteilung der Bewehrungsgarne in sogenannte Segmente berücksichtigt. Das Modell enthält zwei Typen von finiten Elementen: Stabelemente und Verbundelemente. In Längsrichtung werden Stabelemente kettenförmig angeordnet, um das Tragverhalten von Matrix und Bewehrung abzubilden. In Querrichtung sind die Stabelementketten mit Verbundelementen gekoppelt. Das Modell erhält seine Komplexität hauptsächlich aus Nichtlinearitäten in der Materialbeschreibung, z.B. durch begrenzte Zugfestigkeiten von Matrix und Bewehrung, Zugentfestigung der Matrix, Welligkeit der Bewehrung und nichtlineare Verbundgesetze. Neben einer deterministischen Beschreibung des Materialverhaltens beinhaltet das Modell auch eine stochastische Beschreibung auf Grundlage eines Zufallsfeldansatzes. Mit dem Modell können Spannungsverteilungen im Verbundwerkstoff und Eigenschaften der Betonrissentwicklung, z.B. in Form von Rissbreiten und Rissabständen untersucht werden, was in dieser Kombination nur mit wenigen der existierenden Modelle für Textilbeton möglich ist. In vielen der vorhandenen Modelle sind diese Eigenschaften Eingangsgrößen für die Berechnungen und keine Ergebnisse. Darüber hinaus kann anhand des Modells auch das sukzessive Versagen der Bewehrungsgarne studiert werden. Das Modell wurde auf drei verschiedene Versuchstypen angewendet: den Filamentauszugversuch, den Garnauszugversuch und Dehnkörperversuche. Die Berechnungsergebnisse zu den Filamentauszugversuchen zeigten eine gute Übereinstimmung mit experimentellen Resultaten. Zudem wurden Parameterstudien durchgeführt, um Einflüsse aus Geometrieeigenschaften wie der eingebetteten und freien Filamentlänge sowie Materialeigenschaften wie dem Verbund zwischen Matrix und Filament zu untersuchen. Die Berechnungsergebnisse zum Garnauszugversuch demonstrierten die Anwendbarkeit des Modells auf diesen Versuchstyp. Es wurde gezeigt, dass für eine realitätsnahe Abbildung des Versagensverhaltens der Bewehrungsgarne eine relativ feine Auflösung der Bewehrung notwendig ist. Die Berechnungen lieferten die Verteilung von Versagenspositionen in der Bewehrung und die Entwicklung der Degradation der Garne im Belastungsverlauf. Ein Hauptziel der Arbeit war die Untersuchung von Effekten im Zugtragverhalten von Textilbeton, die bisher nicht durch experimentelle Untersuchungen erklärt werden konnten. Daher wurde eine Vielzahl von Parameterstudien zu Dehnkörpern mit mehrfacher Matrixrissbildung, welche das Zugtragverhalten von Textilbeton ähnlich praktischen Anwendungen abbilden, durchgeführt. Die Berechnungsergebnisse zeigten, dass der experimentell beobachtete dreigeteilte Verlauf der Spannungs-Dehnungs-Beziehung von Textilbeton bestehend aus dem ungerissenen Zustand, dem Zustand der Matrixrissbildung und dem Zustand der abgeschlossenen Rissbildung vom Modell wiedergegeben wird. Die beste Übereinstimmung zwischen berechneten und experimentellen Ergebnissen ergab sich unter Einbeziehung von Streuungen in den Materialeigenschaften der Matrix, der Zugentfestigung der Matrix und der Welligkeit der Bewehrung.