I detta projekt har uttrycket av Affibodymolekyler i bioreaktorer av olika volymetriska skalor jämförts för att fastställa om ett tillförlitligt samband mellan de olika bioreaktorernas prestanda kan etableras för att möjliggöra utvecklingen av en nedskalad produktionsmodell för Affibodymolekyler. Baslinjen för jämförelsen i denna studie har varit en enliters- stirred tank reactor (STR) som de andra (mindre) bioreaktorernas prestanda jämfördes med. Jämförelsen av prestanda gjordes genom odling och uttryck av sex olika Affibodymolekyler i replikat i varje bioreaktorstorlek. Prestandan i detta fall hänvisar till produktionen av Affibodymolekyler (mg/L Cellodling) under odlingen, som fastställdes genom ett protokoll för proteinrening av lösligt intracellulärt protein genom affinitetskromatografi och kvantifiering genom absorbans vid 280 nm. De sex olika Affibodymolekyler som har studerats i detta projekt hade tidigare visat sig ha olika uttrycksnivåer, och har här jämförts med varandra i de olika bioreaktorskalorna. De två olika bioreaktorstorlekarna som bedömdes var en 300 mL skakkolv med 50 mL arbetsvolym och en mikrotiterplatta (MTP) med 3 mL arbetsvolym. Dessutom innebar studien en bedömning av två system för en långsam frisättning av kolkälla i odlingarna, ett i varje nedskalad bioreaktorstorlek. Detta utfördes för att delvis efterlikna den kontrollerade fed-batch-kulturen i STR, där koltillförsel kontrollerades med hjälp av en feedprofil. Resultaten visade att proteinuttrycket av metoderna med en långsam frisättning av kolkällor överensstämde närmast med proteinuttrycket i STR. Anpassningen av dessa resultat mot proteinuttrycket hos STR gav i en linjär regression ett R2 på 99,69 % i 3 ml MTP och 97,46 % i 50 ml skakkolven. Slutsatserna som drogs var att SMFP08003 FeedPlate var den bästa kandidaten för en nedskalad modellering av uppströmsprocessen för produktion av Affibodymolekyler. Samt att faktorn att använda en fed-batch-process istället för en batch-process har en större inverkan på proteinuttrycket än skalan av processerna. / In this project the expression of Affibody® molecules in bioreactors of different volumetric scales has been compared, to determine if a reliable relation between the performance of the different bioreactors can be established to allow for the development of a scale-down Affibody® molecule production protocol. The baseline of comparison in this study has been a one litre Stirred Tank Reactor (STR) to which the other (smaller) bioreactors' performance were compared. The performance comparison was achieved by the cultivation and subsequent expression of six different Affibody® molecules in replicates in each bioreactor size. Performance in this case refers to Affibody® molecule production (mg/L culture) during the cultivation, which is assessed by a protocol of protein purification of soluble intracellular protein by affinity chromatography and quantification by 280 nm absorbance. The six different Affibody® molecules studied in this project had previously been found to have different expression levels, and were in this project compared to each other in the different bioreactor scales. The two different bioreactor sizes which were assessed were a 300 mL shake flask with 50 mL working volume and a 3 mL working volume microtiter plate (MTP). In addition, the study involved an assessment of the use of two systems for a slow carbon source release in the cultivations, one in each scale-down bioreactor size. This was performed to partly mimic the controlled feed systems in STRs. The results showed that the protein expression of the methods with a slow carbon source release corresponded most closely with the protein expression in the STR. The fit of these results onto the protein expression of the STR yielded a R2 of 99.69% in the 3 mL MTP and 97.46% in the 50 mL shake flask. The conclusions drawn were that SMFP08003 FeedPlate was the best candidate for scale-down modelling of the upstream process for production of Affibody® Molecules and that the factor of using a fed-batch process instead of a batch process has a larger impact on the protein expression than the scale of either process.

Les jeux vidéo font aujourd'hui partie de la culture de nombreux étudiants au même titre que la télévision, les films ou les livres. Or depuis quelques années, les étudiants se détournent des sciences. La recherche dans le domaine de l'enseignement de l'informatique aborde les problèmes du recrutement et du maintien des étudiants dans les formations informatiques. Une approche prometteuse consiste à utiliser la culture vidéoludique des étudiants pour les motiver à investir du temps dans la pratique de la programmation. Dans ce cadre, les travaux présentés portent sur la conception, la réalisation et l'évaluation d'un jeu sérieux pour l'apprentissage des fondamentaux de la programmation. Ce jeu est basé sur un jeu de stratégie temps réel où la programmation est un moyen d'interaction. Grâce au système Prog&Play, le jeu sérieux a pu être déployé et évalué dans différents contextes d'enseignements.

Pagal Darbo kodekso 109 straipsnį terminuota darbo sutartis nuolatinio pobūdžio darbui atlikti gali būti sudaroma tik išimtiniais atvejais, nustatytais Darbo kodekso 109 straipsnio 2 dalyje. Sudaryti terminuotą darbo sutartį paprastai yra naudingiau darbdaviui, darbuotojo teisės ir interesai, esant terminuotai darbo sutarčiai, tampa labiau pažeidžiami. Viešąjį interesą daugiau atitinka darbuotojo ir darbdavio interesų pusiausvyrą užtikrinančios neterminuotos darbo sutartys, todėl kad nebūtų piktnaudžiaujama terminuotomis sutartimis, įstatymuose nustatyti tam tikri imperatyvieji reikalavimai. Šiame magistro darbe analizuojami terminuotų darbo sutarčių sudarymo, vykdymo bei nutraukimo ypatumai. Magistro darbą sudaro trys pagrindinės dalys. Pirmoje dalyje nagrinėjama bendrosios terminuotos darbo sutarties samprata, požymiai, prielaidos ir ribojimai. Antrojoje magistro darbo dalyje analizuojamas terminuotos darbo sutarties sudarymas, kai darbuotojas priimamas į naujai steigiamą darbo vietą. Atsižvelgiant į Darbo kodekso 109 straipsnio 2 dalies papildymus aptariamos terminuotos darbo sutarties prielaidos, termino nustatymo klausimai, akcentuojant maksimalią terminuotų darbo sutarčių trukmę ir termino nustatymo būdus apžvelgiamas terminuotos darbo sutarties vykdymas. Trečiojoje magistro darbo dalyje nagrinėjamas terminuotos darbo sutarties pasibaigimas. Šioje dalyje, visų pirma, analizuojamos termino pasibaigimo pagrindai, garantijos ir apribojimai bei teisinės terminuotos darbo... [toliau žr. visą tekstą] / The fixed-term employment contract of a permanent nature to do the job can be awarded only in exceptional circumstances set out in Article 109 of the Labour Code, Part 2. To create a fixed-term employment contract is generally beneficial to the employeror the employee's rights and interests under a fixed-term employment contract, it becomes more vulnerable. The public interest is more consistent with open-ended contracts so that were no misuse of fixed-term contracts with certain statutory requirements. This thesis analyzes the fixed-term contracts, performance and terminationfeatures. Master's thesis consists of three main parts. The first part deals with the general concept of fixed-term contract, signs, assumptions and limitations. The second part analyzes the fixed-term employment contract, the worker admitted to a newly set up job. In view of the Labour Code, Article 109 paragraph 2 additions to the contract in question termintuotos assumptions, the term of issues with emphasis on the maximum duration of fixed-term contracts and the term gives an overview of the methods of fixed-term contract. The third part deals with fixed-term contract ends. In this section, in particular, theanalysis of the expiry of the basics, warranties and limitations of the legaltermination of the contract term consequences.

Usage of genetic markers in non-recombining part of chromosome Y has been shown as a eligible tool for a study of history, diversity and migration of population. Applicable markers of chromosome Y are SNP and STR polymorphisms. There were collected 53 unique samples of DNA as a object of this work from unrelated origin males from 9 villages around Klatovy. Samples have been analyzed and its values have been determined by using the 17 STR markers by AmpFLSTR® Yfiler® Direct Kit. In total I have observed 7 different haplogroups. I have resulted samples from villages around Klatovy and they were analyzed by AMOVA. I have compared samples with the surrounding populations in neighborly Federal Republic of Germany, Austria, Central and South Bohemia. There were no significant differences founded in the genetic profile of this population to the surrounding populations.

Este trabalho tem como objetivo a determinação da freqüência alélica de 12 microssatélites do cromossomo Y na população de Araraquara e da Grande São Paulo, tendo em vista a necessidade de ampliação dos dados referentes a estes marcadores devido a sua crescente aplicação em diferentes áreas, entre elas a forense na qual a utilização destes microssatélites torna-se muitas vezes a única ferramenta disponível para resolução de casos. Para isto foram tipados 200 indivíduos, que não apresentavam relação de parentesco, divididos em quatro grupos de acordo com autoclassificação de cor (branco, preto, pardo ou oriental). Foram coletadas destes indivíduos amostras de sangue ou saliva a partir das quais foi feita extração do DNA utilizando diferentes protocolos de acordo com o tipo de amostra, seguida da amplificação dos 12 locos do cromossomo Y através do PowerPlex® Y System (Promega) de acordo com instruções do fabricante. Os produtos da amplificação foram submetidos a eletroforese em gel de poliacrilamida desnaturante a 6%, no seqüenciador ABI377 (Applied Biosystems) para obtenção dos perfis de cada loco. Os quais foram analisados com a utilização do software GeneScan ver. 2.1 (Applied Biosystems). Foi realizado o cálculo das freqüências alélicas e diversidade gênica de cada loco, assim como da diversidade haplotípica e capacidade de discriminação para cada grupo e para a amostra total. A comparação entre os resultados obtidos demonstrou que a variação dentro de cada grupo é maior que a variação entre os grupos. Os resultados obtidos foram enviados ao banco de dados mundial do cromossomo Y (Y-STR Haplotype Reference Database). / The aim of this study is to determine the allelic frequency of 12 microsatellites of the Y chromosome in Grande São Paulo and Araraquara population, in face of the amplification necessity of these markers data due to the increasing application of these markers on different fields, including the forensic on which the use of them is sometimes the only way to solve crime cases. For this purpose it was typed 200 unrelated individuals divided according to self report in four groups based on color skin (white, black, mulatto or yellow). Blood or buccal swab samples were collected and submitted to DNA extraction with different protocols according to the kind of sample. Subsequent amplification of 12 Y-STR was proceeded using the PowerPlex® Y System (Promega) following the manufacture’s protocol. The amplification products were submitted to electrophoresis in 6% polyacrilamid gel on ABI377 sequencer (Applied Biosystems) to obtain the profile of each locus. The results were analyzed with GeneScan ver. 2.1 software (Applied Biosystems). The allelic frequency and gene diversity of each locus as well as the haplotypic diversity and discrimination capacity was calculated for each group and for total sample. The comparison among the results showed that the variation inside the groups is higher than between groups. The haplotypes observed on this sample were sent to Y-STR Haplotype Reference Database.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:31:20Z No. of bitstreams: 1 TES_ANDRE_ZORATTO_GASTALDO_COMPLETO.pdf: 1361129 bytes, checksum: dbc7c8c4f5ab004118129ecf678044b8 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:31:27Z (GMT) No. of bitstreams: 1 TES_ANDRE_ZORATTO_GASTALDO_COMPLETO.pdf: 1361129 bytes, checksum: dbc7c8c4f5ab004118129ecf678044b8 (MD5) / Made available in DSpace on 2017-06-30T18:31:34Z (GMT). No. of bitstreams: 1 TES_ANDRE_ZORATTO_GASTALDO_COMPLETO.pdf: 1361129 bytes, checksum: dbc7c8c4f5ab004118129ecf678044b8 (MD5) Previous issue date: 2017-03-21 / The evolution in human identification through the study of genetic markers STR (Short Tandem Repeats) induced and propitiated the growth also of animal genetic identification. However, contrary to what occurs in the human domain, animal molecular identification still lacks validation of analysis systems and population studies to be considered reliable to the degree required. Only recently the International Society for Animal Genetics (ISAG) has recommended STR markers suitable for individual discrimination in domestic animal species. Accurate animal genetic identification is sought to provide entities, associations, breeders and owners in general with assurances that their animals are in fact derived from reliable breeders. Only reliable and authentic genealogical records will ensure, for example, efficiency in genetic breeding processes. Even though it is a promising area, there are currently only two options in the market for commercial kits for animal genotyping. But its use has been deferred by specialized laboratories in the field, which end up preparing their own identification panels, using the recommended genetic markers to meet their local demands more specifically. However, if the frequencies of the alleles present in these genetic markers in the study population are not known and the real power of discrimination that they provide is not estimated, the analyzes performed and the results obtained may generate misleading or imprecise interpretations. In the present study, three panels of STR genetic markers were used with all loci recommended by ISAG, capable of identifying equine, bovine and canine individuals. Allelic frequencies and other parameters such as heterozygosity, polymorphism information content, power of exclusion and power of discrimination were obtained from significant samples in breeds present in Rio Grande do Sul (Southern Brazil), Uruguay and Paraguay, to be used in the practice of animal genetics for identification purposes. In addition, comparative population studies within and among breeds were also carried out, in order to evaluate the genetic diversity of the animal populations studied. / O avan?o da identifica??o molecular humana pelo estudo dos marcadores gen?ticos STR (Short Tandem Repeats) induziu e propiciou o crescimento tamb?m da identifica??o gen?tica animal. Contudo, ao contr?rio da ?rea humana, a identifica??o molecular animal ainda carece de valida??es dos sistemas de an?lise e de estudos populacionais para que seja considerada fidedigna no grau que se necessita. Apenas recentemente a ISAG (International Society for Animal Genetics) recomendou marcadores STR adequados para discrimina??o individual em esp?cies de animais dom?sticos. A identifica??o gen?tica animal exata e precisa ? buscada para fornecer, a entidades, associa??es, criadores e propriet?rios em geral, garantias de que seus animais s?o, de fato, oriundos de reprodutores confi?veis. Apenas registros geneal?gicos fidedignos e aut?nticos garantir?o, por exemplo, efici?ncia nos processos de melhoramento gen?tico. Mesmo sendo uma ?rea promissora, neste momento h? somente duas op??es no mercado de kits comerciais para genotipagem animal. Mas seu uso tem sido preterido por laborat?rios do ramo, os quais acabam preparando seus pr?prios pain?is (in house) de identifica??o, utilizando os marcadores gen?ticos recomendados para atender suas demandas locais de forma customizada. Contudo, se n?o forem conhecidas as frequ?ncias dos alelos presentes nestes marcadores gen?ticos na popula??o em estudo e n?o for estimado o real poder de discrimina??o que estes fornecem, as an?lises realizadas e os resultados obtidos poder?o gerar interpreta??es equivocadas ou imprecisas. No presente trabalho, foram utilizados tr?s pain?is de marcadores gen?ticos STR com todos os loci recomendados pela ISAG, capazes de identificar indiv?duos equinos, bovinos e caninos. Frequ?ncias al?licas e outros par?metros como heterozigosidade, conte?do de informa??o de polimorfismo, poder de exclus?o e de discrimina??o foram obtidos a partir de amostras significativas em ra?as presentes no Rio Grande do Sul/Brasil, no Uruguai e no Paraguai, para que sejam usados na pr?tica da gen?tica animal com fins de identifica??o. Al?m disso, estudos populacionais comparativos dentro e entre ra?as tamb?m foram realizados, com o intuito de avaliar a diversidade gen?tica das popula??es animais estudadas.

Submitted by Erika Demachki (erikademachki@gmail.com) on 2015-02-27T18:12:53Z No. of bitstreams: 2 Tese - José Santana da Silva - 2014.pdf: 2049842 bytes, checksum: 513ce3d0c825870ec2294bff10b4a1f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2015-02-27T18:13:14Z (GMT) No. of bitstreams: 2 Tese - José Santana da Silva - 2014.pdf: 2049842 bytes, checksum: 513ce3d0c825870ec2294bff10b4a1f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2015-02-27T18:13:14Z (GMT). No. of bitstreams: 2 Tese - José Santana da Silva - 2014.pdf: 2049842 bytes, checksum: 513ce3d0c825870ec2294bff10b4a1f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-10-30 / Outros / This thesis is a history of union organization of workers in agriculture in the state of Goiás, between 1963 and 1992, that is, the foundation of the first unions to extinction of Rural Workers Trade Union Opposition. The starting point is the origin of the unions in the history of modern societies, created by the working class to combat the exploitation of its workforce. The Following are the definitions of bureaucracy, while social class relations and bureaucratic red tape, with the fundamental references formulations of Marx, Viana and Tragtenberg. The contributions of Weber and Lefort are secondary references. In characterizing the bureaucratization of unions, the analyzes of Luxembourg and Pannekoek are basic. Reconstitution of the bureaucratization of the union organization process in Brazil is based on works of national authors and analysis of trade union legislation. The theoretical and historical explanation is performed in the first chapter. The second is devoted to the emergence of rural unionism in the state of Goiás In the third and fourth story is addressed to the Federation of Agricultural Workers in the State of Goiás – Fetaeg – including the relationship with the Trade Union Opposition and the struggles of peasants and agricultural workers. In addition to bibliographical sources, journalistic sources, official documents of the Ministry of Labor, the state government, the unions of rural workers and Fetaeg were used. Some oral testimonies were used in a complementary manner. / A presente tese consiste numa história da organização sindical dos trabalhadores na agricultura no estado de Goiás, entre 1963 e 1992, isto é, da fundação dos primeiros sindicatos à extinção da Oposição Sindical dos Trabalhadores Rurais. O ponto de partida é a origem dos sindicatos na história das sociedades modernas, criados pela classe operária para combater a exploração da sua força de trabalho. A seguir, são apresentadas as definições dos conceitos de burocracia, enquanto classe social, relações burocráticas e burocratização, tendo como referências fundamentais as formulações de Marx, Viana e Tragtenberg. As contribuições de Weber e de Lefort são referências secundárias. Na caracterização da burocratização dos sindicatos, as análises de Luxemburgo e de Pannekoek são basilares. A reconstituição do processo de burocratização da organização sindical no Brasil é feita com base em trabalhos de autores nacionais e na análise da legislação sindical. A explanação teórica e histórica é realizada no primeiro capítulo. O segundo é dedicado ao surgimento do sindicalismo rural no estado de Goiás. No terceiro e no quarto é abordada a história da Federação dos Trabalhadores na Agricultura no Estado de Goiás – FETAEG –, incluindo a relação com a Oposição Sindical e com as lutas dos camponeses e assalariados agrícolas. Além das fontes bibliográficas, foram utilizadas fontes jornalísticas, documentos oficiais do Ministério do Trabalho, do governo estadual, dos sindicatos de trabalhadores rurais e da FETAEG. Alguns depoimentos orais foram usados em caráter complementar.

No description available.

Europos Bendrija nuo įkūrimo, kuriuo buvo siekiama išimtinai ekonominių tikslų, žymiai pasikeitė. Dabar ji yra įvardijama kaip savita teisinė sistema, sukurianti teisies ir pareigas ne tik valstybėms narėms, bet ir privatiems asmenims. Europos Bendrijoje, paremtoje teisės viršenybės principu, turi būti numatytas Bendrijos teisės aktų teisėtumo priežiūros procedūrų mechanizmas. Privačių asmenų teisė inicijuoti teisėtumo priežiūros procedūras bei locus standi (teisės į ieškinį) sąlygos yra tiesiogiai numatytos Sutarties 230(4) straipsnyje. Lietuviškoje literatūroje privačių asmenų teisė pateikti ieškinį yra aptarta labai glaustai. Apsiribota tik trumpu kliūčių pateikti ieškinį dėl panaikinimo aprašymu bei pagrindinių bylų paminėjimu. Ir nors užsienio literatūroje privačių asmenų locus standi sąlygos yra plačiai išnagrinėtos, dar nėra atlikta tyrimų dėl naujoje Lisabonos sutartyje numatytų pakeitimų. Šiame moksliniame tiriamajame darbe siekiama atskleisti privataus asmens sąvoką, išanalizuoti reikalavimus teisės aktams, dėl kurių yra galimi ieškiniai dėl panaikinimo, išnagrinėti locus standi sąlygas, kurias turi atitikti privatus asmuo pateikiantis ieškinį dėl Bendrijos teisės akto panaikinimo. Taip pat aptariama 234 ir 241 straipsnių, kaip papildančių teisėtumo priežiūros Bendrijoje sistemą, reikšmė bei 230(4) straipsnio pakeitimų, numatytų Lisabonos sutartyje prasmė. Šio darbo tikslas yra išanalizuoti privačių asmenų galimybes inicijuoti teisėtumo priežiūros procedūras... [toliau žr. visą tekstą] / The European Community (EC) since its establishment as a pure economic community with the goal to bring about economic integration between few European countries now has created a platform for international corporation and evolved in having influence on private persons. As based on the rule of law, the Community must have a mechanism for testing the substantial and procedural legality of measures, adopted by Community institutions. The legal basis for bringing an action for annulment is the 230 article of the EC Treaty. The fourth paragraph of this article determines the circumstances under which natural and legal persons may bring an action for annulment. In foreign literature the question of standing of private applicants is often analysed, though no studies are yet made on the amendments of Lisabon treaty. As for Lithuanian literature, the standing of private parties is not much discussed, so the analysis made in this paper is practicable for Lithuanian individuals. The master’s thesis seeks to analyse what is a private person, the measures, that may be challenged and the conditions a private applicant must fulfill in order to have locus standi (a right to bring an action). It also studies the purpose and importance of articles 234 and 241 EC, as these are also measures for initiating a review procedure in the Court of Justice. The purpose of this paper is to settle weather the obstacles and difficulties a private person meets in order to bring an action for annulment... [to full text]

It is common practice for DNA STR profiles to be analyzed using an analytical threshold (AT), but as more low template DNA (LT-DNA) samples are tested it has become evident that these thresholds do not adequately separate signal from noise. In order to confidently examine LT-DNA samples, the behavior and characteristics of the background noise of STR profiles must be better understood. Thus, the background noise of single source LT-DNA STR profiles were examined to characterize the noise distribution and determine how it changes with DNA template mass and injection time. Current noise models typically assume the noise is independent of fragment size but, given the tendency of the baseline noise to increase with template amount, it is important to establish whether the baseline noise is randomly found throughout the capillary electrophoresis (CE) run or whether it is situated in specific regions of the electropherogram. While it has been shown that the baseline noise of negative samples does not behave similarly to the baseline noise of profiles generated using optimal levels of DNA, the ATs determined using negative samples have shown to be similar to those developed with near-zero, low template mass samples. The distinction between low-template samples, where the noise is consistent regardless of target mass, and standard samples could be made at approximately 0.063 ng for samples amplified using the Identifiler^TM Plus amplification kit (29 cycle protocol), and injected for 5 and 10 seconds. At amplification target masses greater than 0.063 ng, the average noise peak height increased and began to plateau between 0.5 and 1.0 ng for samples injected for 5 and 10 seconds. To examine the time dependent nature of the baseline noise, the baselines of over 400 profiles were combined onto one axis for each target mass and each injection time. Areas of reproducibly higher noise peak heights were identified as areas of potential non-specific amplified product. When the samples were injected for five seconds, the baseline noise did not appear to be time dependent. However, when the samples were injected for either 10 or 20 seconds, there were three areas that exhibited an increase in noise; these areas were identified at 118 bases in green, 231 bases in yellow, and 106 bases in red. If a probabilistic analysis or AT is to be employed for DNA interpretation, consideration must be given as to how the validation or calibration samples are prepared. Ideally the validation data should include all the variation seen within typical samples. To this end, a study was performed to examine possible sources of variation in the baseline noise within the electropherogram. Specifically, three samples were prepared at seven target masses using four different kit lots, four capillary lots, in four amplification batches or four injection batches. The distribution of the noise peak heights in the blue and green channels for samples with variable capillary lots, amplifications, and injections were similar, but the distribution of the noise heights for samples with variable kit lots was shifted. This shift in the distribution of the samples with variable kit lots was due to the average peak height of the individual kit lots varying by approximately two. The yellow and red channels showed a general agreement between the distributions of the samples run with variable kit lots, amplifications, and injections, but the samples run with various capillary lots had a distribution shifted to the left. When the distribution of the noise height for each capillary was examined, the average peak height variation was less than two RFU between capillary lots. Use of a probabilistic method requires an accurate description of the distribution of the baseline noise. Three distributions were tested: Gaussian, log-normal, and Poisson. The Poisson distribution did not approximate the noise distributions well. The log-normal distribution was a better approximation than the Gaussian resulting in a smaller sum of the residuals squared. It was also shown that the distributions impacted the probability that a peak was noise; though how significant of an impact this difference makes on the final probability of an entire STR profile was not determined and may be of interest for future studies.