The thesis " Physiotherapy of soft hand weavings disorders resulting from postneuritic over freight" is bent on weightiest consequence of over freight of hand flexor sinews, which is rupture and on one of weightiest consequences of accident states of sinews, which is transcision. Rupture and transcision of hand flexor sinews is cured in a similar surgical way, by suture and coincident is as well the subsequent alteration postoperative care. The aim of my diploma work that ties on my baccalaureate work, is rounded up from the expert literature, especially the foreign one, researching the information of possibilities and meaning of rehabilitative care in those specific problems and these information then virtually compare with the choice study sample inmates. I hereto directed everything, so as I could in the end answers the questions: what meaning has rehabilitation and whether there is any universal method that could be applied in the appropriate manner. In its experimental parts the diploma work succeeded to prove the evidence, that the rehabilitation holds irreplaceable space in therapy of hand flexor sinews after effected sutures. The study proves the obvious difference in results between the group that passed the rehabilitation therapy and the group that the rehabilitation didn't undergo. The tracked...

A durotomia acidental, na cirurgia da coluna lombar, é uma das mais comuns complicações, com uma prevalência de 1 a 17%. As fraturas da coluna também podem apresentar-se com lesão dural, chegando a 19% nas fraturas tipo explosão da coluna torácica ou lombar com lesão associada da lâmina vertebral. Procurou-se avaliar as alterações medulares que ocorreram apos a reparação de lesão dural com pontos do tipo simples, com cola de fibrina e com colágeno bovino, através de análise histológica. Manteve-se um grupo controle, sem reparação. Utilizaram-se 70 ratos da raça Wistar, sendo que 34 foram excluídos por problemas anestésicos ou intra-operatórios. Mantiveram-se nove ratos por grupo. Abordaram-se os segmentos vertebrais T8 e T9, para efetuar a lesão, que foi reparada pelos três diferentes métodos avaliados. Os animais permaneceram confinados por 24 dias, sendo submetidos à eutanásia com a coleta de material para análise histológica. O Serviço de Patologia avaliou e graduou (ausente, discreto, moderado e acentuado) os casos quanto à hiperemia, degeneração medular, necrose e infiltrado celular. De forma geral, as técnicas de reparo apresentaram resultados com maior grau de alteração, principalmente em relação ao infiltrado celular, onde todas as técnicas mostraram resultados bem piores. A sutura apresentou graus mais severos de necrose, hiperemia e infiltrado celular. A membrana de colágeno apresentou resultados com graus mais elevados de alteração em relação à hiperemia, à degeneração da substância nervosa e ao infiltrado celular. A reparação com cola de fibrina apresentou piora em relação ao grau de necrose e de infiltrado celular. A presença de alterações medulares em todos os grupos, até mesmo no grupo controle, levou a cogitar algumas possíveis causas para os resultados: as alterações medulares seriam geradas pela técnica cirúrgica ocorrendo uma lesão iatrogênica medular, mas sem repercussão clinica; os materiais estudados poderiam causar alterações no tecido neural; o simples contato do tecido neural com o meio extra-dural, após a lesão, seria a causa das alterações levando à mesma cascata de alterações que ocorre nas lesões medulares traumáticas. Apesar de todos os grupos apresentarem alterações medulares, ficou evidente que as de maior intensidade ocorreram nos grupos que utilizaram materiais de fechamento dural. Os métodos de reparação dural ainda não são os ideais, mas existe o consenso que a lesão deve ser reparada sempre na forma aguda, a fim de evitar e prevenir complicações e seqüelas. / This study aimed to evaluate spinal cord alterations after dural repair with: simple interrupted suture, collagen membrane or fibrin glue using histopathogical analysis. All activities were performed in the São Paulo University Medical School General Hospital Traumatology and Orthopedic Division. Seventy Wistar rats were used, but 34 were excluded due to anesthetic or surgery problems. Animals were kept isolated for 24 days, then killed and histological samples collected. Pathology Division assessed and graduated hyperemia, spinal cord degeneration, necrosis and cellular infiltration. Repair techniques provided higher alteration, mostly in cellular infiltration. Suture showed more severe necrosis, hyperemia and cellular infiltrates graduations. Collagen membrane provided higher alterations related to hyperemia, nerve degeneration and cellular infiltrates. Fibrin glue reparation had worse results in necrosis and cellular infiltrates graduation.Spine cord alterations in all groups, including control group, made us suppose possible reasons for the results: spine cord changes would be caused by surgical technique as iatrogenic injury, without clinical meaning; studied material would cause alterations in neural tissue; contact of neural tissue with extra dural environment, after injury, leading to the same mechanisms present in spinal cord traumatic injury. Despite all groups showed spinal cord alterations it was clear a higher intensity of injury when dural closing material were used. There is no ideal choice for dural repair material and more research is warranted to find better form of repairing dura mater.

The Southeastern US is an ideal location to explore the interactions between mountain-building, rifting, magmatism and intraplate deformation. It experienced the formation of the Southern Appalachians over multiple episodes of orogenesis, continental rifting that formed the South Georgia Rift Basin, and widespread magmatism associated with the Central Atlantic Magmatic Province (CAMP). CAMP was followed by the breakup of Pangea, but the suture between Laurentia and Gondwana from the Appalachian orogeny is preserved in the crust of the Southeastern US. Intraplate seismicity indicates ongoing deformation in the Southeastern US today, but the mechanisms controlling this seismicity are poorly understood. This thesis uses seismic constraints to examine the tectonic history of the Southeastern United States (US). We use new wide-angle refraction seismic data to model crustal and upper mantle velocities in order to investigate the structures formed by mountain-building, rifting, and magmatism. Broadband seismic data are then used to detect and characterize earthquakes in the central Georgia-South Carolina region. Wide-angle seismic data were collected on three profiles crossing major geological features in Georgia to investigate the tectonic history of the Southeastern United States as a part of the SUwanee Suture and GA Rift basin experiment (SUGAR) project. We model VP and VS of the crust and upper mantle on SUGAR Line 2, which extends from the Inner Piedmont to the Georgia coast. We identify a north to south decrease in upper crustal VP/VS at the Higgins-Zietz magnetic boundary, which other recent studies have identified as the location of the suture between Laurentia and Gondwana. This boundary also lies near the northwestern edge of the South Georgia Rift Basin, the southeastern termination of the low velocity zone interpreted as the Appalachian detachment, and localized crustal thinning. Together, these results provide new evidence in support of the Alleghanian suture being located at the Higgins-Zietz magnetic boundary, and suggest that this orogenic boundary influenced the location of subsequent rifting. We compare the VP structures of two SUGAR wide-angle transects that cross western and eastern segments of the South Georgia Rift, respectively. Elevated (>7.0 km/s) lower crustal velocities are observed where the rift basin sedimentary fill is thickest and the crust is most thinned. The quantities of mafic magmatic intrusions are consistent with decompression melting at modestly elevated mantle potential temperatures, such as those estimated for CAMP intrusions. These results suggest that, in contrast with the widespread CAMP-aged magmatism at the Earth’s surface, lower crustal magmatic intrusions in the Southeastern US are limited and localized in areas that experienced extension. These new constraints on the velocity structure and tectonic history of the Southeastern United States are then applied to understand earthquakes in the region today. Using broadband seismic data, we find that earthquakes southeast of the Eastern Tennessee Seismic Zone are concentrated within the Carolina Terrane, a particularly heterogeneous accreted terrane of the Southern Appalachians. Within this terrane, seismicity concentrates near rivers and reservoirs, including a sequence of earthquakes in 2013 associated with an increase in water levels at Thurmond Lake on the Georgia-South Carolina border. Focal mechanisms suggest that the earthquakes are occurring on structures that are oblique to the trend of the Appalachians that are more optimally oriented in the modern stress regime.

BACKGROUND: Pleiotropy is a biological phenomenon of a single gene exhibiting influence over several different seemingly disparate phenotypes. This phenomenon poses significant challenges to fully understanding the etiologies of many different Mendelian diseases. Two such Mendelian diseases are Idiopathic Hypogonadotropic Hypogonadism (IHH) and Craniosynostosis (CS). IHH results from the failure of differentiation, migration, secretion, or action of the GnRH neurons resulting in absent puberty and infertility. CS is characterized by premature fusion of one or more of the cranial sutures resulting in dysmorphic shape of the skull that can lead to life-threatening raised intercranial pressure requiring surgical intervention. Thus far, 77 genes have been implicated in IHH and 128 genes have been implicated CS, both representing ~50% of the cases in their respective diseases. Recent research has suggested a shared molecular landscape in CS and IHH but the full ensemble of this overlap is not known. OBJECTIVE: This study will attempt to utilize human genetics, bioinformatics, statistics, phenotype data of IHH patients, and the prior literature in order to ascertain the full extent of the shared biology of IHH and CS. METHODS: The gene sets of both IHH and CS were used in gene overlap statistical analysis to investigate shared genetics. Whole exome sequencing data from 1,395 patients from the IHH cohort of the Massachusetts General Hospital were used for gene-variant burden analysis to determine genetic overlap with CS. Detailed physician notes from this cohort were used to determine phenotypic presence of CS in IHH. Conversely, evidence of reproductive phenotypes in genetically characterized CS patients was gathered from the reported CS gene literature. The CS and IHH gene sets were also bioinformatically analyzed using both the Metascape and DAVID bioinformatic platforms for pathway annotation, protein-protein interaction (PPI), and functional interactions to provide evidence for the mechanism of shared biology. RESULTS: Of the 128 CS genes and 77 IHH genes, 4 were determined to be causal for both diseases with a further 3 considered as potentially causal candidates for both diseases. The 4 overlapping causal genes were tested using three different methods and this overlap was determined to be of statistical significance (p<0.05). Furthermore, the phenotypic review revealed that while there was not a significant enrichment for CS phenotypes in the IHH cohort, the literature review yielded 49 of 128 CS genes that were reported with phenotypic evidence of failure of the hypothalamic-pituitary portion of the HPG axis. Gene-variant burden analysis yielded nominal (p<0.05) enrichment in the IHH cohort for 17 CS genes, of which 3 were significant after Bonferroni multiple testing correction (p<0.00039). The CS/IHH gene sets were both enriched in 44 shared pathways according to Metascape and 17 shared pathways according to DAVID. PPI analysis yielded 3 shared communities between the two disorders with enrichment in fibroblast signaling, ossification, and cardiac chamber development. CONCLUSIONS: The shared biology between IHH and CS was significantly greater than what was previously appreciated. Shared pathways of the two gene sets point toward the neural crest origin of subpopulations of the GnRH neuron and cranial suture osteoblast as a possible foundation for this shared biology, as well as the migratory nature of these two cells and the role that many genes in both gene sets play in cellular motility. Several CS genes emerge as candidates for IHH and must be individually evaluated. Functional studies should be used to confirm and further unravel the underlying mechanisms for the biological overlap between these two diseases. This study may provide a model for preemptive in silico work prior to more expensive in vitro or in vivo studies of pleiotropy.

Objetivo: Evaluación de la osificación de la sutura media palatina y la discrepancia transversal maxilar en pacientes de 18 a 40 años de un centro radiológico de Lima - Perú. Materiales y métodos: Se realizó un estudio observacional de corte transversal. La muestra estuvo constituída por 234 tomografías computarizadas de haz cónico en pacientes de 18 a 40 años de edad, donde se realizó el análisis de Penn CBCT para la evaluación de la discrepancia transversal maxilar mientras que la clasificación de Angelieri se utilizó para evaluar la osificación de la sutura media palatina. Se utilizaron las pruebas de Chi cuadrado, U Mann Whitney y Correlación de Spearman para evaluar si existe relación entre las variables de estudio. Resultados: La discrepancia transversal fue de 7.27mm. Por otro lado, se encontró que en el rango de 18 a 28 años de edad prevalece el estado B con 65 pacientes, mientras que en el rango de 29 a 40 años hubieron 50 pacientes del mismo estado. Así mismo, existe asociación entre la discrepancia transversal maxilar con el género, mientras que la osificación de la sutura media palatina no está relacionada al género. Conclusiones: Se concluye que no se encontró una asociación entre la osificación de la sutura media palatina, la discrepancia transversal y la edad, esto indica que cada variable es independiente. Por otro lado, existe asociación entre la discrepancia transversal maxilar y el género. / Objective: Evaluation of the ossification of the mid-palatal suture and the maxillary transverse discrepancy in patients aged 18 to 40 years from a radiological center in Lima-Peru. Materials and methods: An observational cross-sectional study was conducted. The sample consisted of 234 conical beam CT scans in patients aged 18 to 40 years, where the CBCT Penn analysis was performed for the evaluation of the maxillary transverse discrepancy, while the Angelieri classification was used to evaluate the ossification of the mid palatal suture. Chi Square, Mann–Whitney U, and Spearman Correlation tests were used to assess whether there was a relationship between the study variables. Results: The transverse discrepancy was 7.27 mm. On the other hand, it was found that in the range of 18 to 28 years of age, state B prevails with 65 patients, while in the range of 29 to 40 years there were 50 patients of the same state. Likewise, there was an association between the maxillary transverse discrepancy with the gender, while the ossification of the mid-palatal suture was not related with the gender. Conclusions: It was concluded that no association was found between the ossification of the mid-palatal suture, the transverse discrepancy, and age; this indicates that each variable is independent. On the other hand, there was an association between maxillary transversal discrepancy and gender. / Tesis

Introduction: Skeletal expansion has been a treatment modality in orthodontics and orthopedics to correct skeletal transverse discrepancies with maxillary constriction. The utilization of microimplants in conjunction with these palatal expanders offers a higher degree of pure skeletal expansion and minimizes the dental side effects. The purpose of this study is to evaluate the changes of the hard and soft tissues of the face after skeletal expansion for orthodontics. Methods: 36 patients who had received successful expansion with a microimplant assisted rapid palatal expander were compared to their pre-expansion records. All patients received CBCTs from which a 3-D analysis configuration was created to trace hard and soft tissue landmarks of the midface and nasal cavity regions. 3 judges analyzed each set of records and the average was used to calculate the amount of expansion experienced at each anatomical region. A paired T-test and Wilcoxon signed-rank test were used for statistical comparison between time points. Results: Expansion can affect all of the midfacial hard tissues that support the overlying soft tissues. Increases in skeletal width from the Frontozygomatic suture down to the maxillary alveolar bone were all significant. The nasal cavity increased in width in all locations measured. Soft tissue changes were significant at the base of the ala suggesting a widening of the nose with expansion therapy. Conclusion: Maxillary expansion with microimplant assisted expanders can have skeletal changes throughout the entire midface and may affect the width of the nasal cavity. Soft tissue changes were less pronounced, and though a widening of the base of the nose may be expected this may not be noticed by the patient.

The aim of this project was to address the food waste problem, particularly bread waste, to some extent by producing monofilaments obtained from wet spinning of fungal hydrogel through the cultivation of Rhizopus delemar on bread waste. The project had two phases. Firstly, the possibility of production of chitosan fiber with wet spinning (using different acids) was evaluated, the process was optimized, and then applied to the production of fungal fiber. Regarding first stage of the project, adipic acid, a non-toxic solvent with two carboxyl groups, was used as acting physical crosslinker between the chitosan chains, resulting in improving properties of the monofilaments. Adipic acid performance was compared with conventional solvents, such as citric, lactic, and acetic acids. By injecting chitosan solutions into a coagulation bath (EtOH or NaOH 1M or EtOH-NaOH or H2SO4-EtOH), monofilaments were formed. Scanning electron microscopy showed that uniform chitosan monofilaments with smooth surface were formed using adipic and lactic acids. In general, fibers obtained from adipic acid displayed higher mechanical strength (Young’s modulus of 4.45 GPa and tensile strength of 147.9 MPa) than that of monofilaments produced using conventional solvents. Fiber dewatering with EtOH before drying led to greater fiber diameter and lower mechanical strength. As the second stage of this study, Rhizopus delemar was cultivated on bread waste in shake flasks and 1.3 M3 bioreactor. While different combinations of ground bread and K2HPO4 was used as the substrate for shake flask cultivations, white bread waste without K2HPO4 was utilized for scaling up the process, mostly due to the Glucosamine (GlcN) and N-acetyl-glucosamine (GlcNAc) content in the fungal cell wall. GlcN and GlcNA content obtained from ground bread was remarkably higher than that of obtained from combinations of ground bread and K2HPO4 as the substrate. Cultivation in 1.3 M3 bioreactor resulted in about 36 kg wet biomass with a mean of 14.88% dry weight, indicating 5.95 g biomass/L. The biomass yield of 0.15 g dry biomass/g dry bread was achieved. Alkali insoluble material (AIM) was obtained by alkali treatment of biomass. Fungal hydrogel was prepared by adding adipic and lactic acid to AIM, followed by grinding treatment. While hydrogels treated with lactic acid showed better spinnability and gelling ability, the one from adipic acid was not uniform to be wet spun. Considering hydrogels treated with lactic acid, the optimum grinding cycle for more spinnable hydrogel was 6 negative cycles, contributing to the fibers with the tensile strength of around 82 MPa. Such fibers showed antibacterial property against Escherichia coli, making them as a good option for suture applications. However, further in vitro and in vivo trials are essential to test the fungal fiber for such applications.

No description available.

Manuscript No. 1 Recent research has indicated that low nest success and juvenile survival of Greater Sage-Grouse may be responsible for population declines. Recent technological advances in micro-transmitters have made radio-telemetry studies on Sage-Grouse chicks more common. Radio-telemetry enables monitoring of individual chicks and broods during a critical period of their life history. The exact cause of low chick recruitment in Strawberry Valley has not been well understood. In 2006, a chick mortality study using micro-transmitters was initiated to (1) determine the causes of chick mortality, (2) calculate overall chick survival, (3) compare chick survival in the Strawberry Valley population to published reports, (4) monitor brood movements, and (5) suggest management strategies for mitigation of chick mortality. Survival data on radio-marked chicks were analyzed using a known fate model in program MARK. Chick survival in Strawberry Valley was greater than all reported estimates from other studies. Our study did not identify any unsuspected causes of chick mortality, and the cumulative effect of stressing chicks, hens, and broods was not deemed worth the benefit, especially in a population recovery setting like Strawberry Valley. We do not recommend the use of radio-telemetry on Sage-Grouse chicks in recovering or sensitive populations. Manuscript No. 2 In 2003, we began translocating Greater Sage-Grouse into the Strawberry Valley of central Utah, in an attempt to recover the dwindling population found therein. Prior to 2006 all translocated Sage-Grouse were released within 250 m of the only active lek in Strawberry Valley while males were actively strutting. A prolonged winter in 2006 delayed normal lekking activity in Strawberry Valley. As a result 61 (59%) of the 103 sage-grouse translocated in 2006 were not released near an active lek. We analyzed the influence that release timing, hen age, body mass, and source population had on mortality, flocking, and dispersal distance of translocated hens in 2006. We found that mortality and flocking rates were not influenced by release timing, hen age, body mass, or source population. Dispersal distances for hens released near a lek with actively strutting males were significantly less than distances of hens released near an inactive lek. We believe that releasing translocated Sage-Grouse near a lek with actively strutting males is an essential technique for Greater Sage-Grouse translocations. We recommend that other Sage-Grouse translocation efforts employ this method to increase the likelihood of success.

Objective: To evaluate the relative position of the femur and tibia in cranial cruciate ligament (CCL) intact stifles, CCL deficient stifles, and stifles following a novel extracapsular procedure (lateral extracapsular suture system or LESSa) under load at specific joint angles. Study Design: In vitro biomechanical study. Methods: Twenty pelvic limbs from 11 dogs were used to evaluate the relative position of the femur and tibia between 3 stifle conditions (CCL intact, CCL deficient, and LESSa treated) at a load of 30 % and stifle angles of 125", 135", and 145" using electromagnetic tracking sensors. Results: Cranial cruciate ligament deficient stifles had significantly greater (p <0.0001) cranial displacement and internal rotation of the tibia relative to the femur than CCL intact stifles or LESSa treated stifles at all stifle angles. Cranial displacement of the tibia relative to the femur for CCL intact and LESSa treated were not significantly different from one another at stifle angles of 125", but were significantly different at stifle angles of 135" (p = 0.0182) and 145" (p = 0.0012). There was no significant difference in internal rotation of the tibia relative to the femur between CCL intact and LESSa treated stifles at any of the stifle angles. Conclusion: LESSa effectively decreases cranial tibial displacement and eliminates internal rotation of the tibia relative / Master of Science