Advanced communication with capabilities such as voice, data, and messaging usually requires an infrastructure with base stations, servers, and etcetera. The TETRA technology offers such communication not only in TMO (infrastructure-based network) but also in DMO where all nodes communicate directly (or via a repeater) with each other. This master’s thesis concerns messaging (specifically short messages) in a dynamic multi link TETRA DMO network. It examines what type of messaging technique to use and how to do path selection. The messages will be clear text, status, and GPS location information. The solution is implemented as a part of the ISIS software (which is developed by Know IT Dataunit). The planned multi link-part of the thesis could not be tested, so there is no implementation or evaluation of this. The evaluation of the implementation concerning sending and reception of messages shows that the proposed solution fulfils the demands for this kind of product. During a four day long test, messages (short text messages and positioning messages) were sent and received while a normal number of voice conversations took place, without packet loss. / Avancerad kommunikation med funktioner såsom röstsamtal, dataöverföring samt meddelandetjänster kräver ofta en infrastruktur med basstationer, servrar etcetera. TETRA-tekniken erbjuder sådan kommunikation, inte bara i TMO (infrastrukturbaserade nätverk), utan även i DMO där alla noder kommunicerar direkt (eller via en repeater) med varandra. Detta examensarbete undersöker hanteringen av meddelanden (framförallt korta meddelanden) i ett dynamiskt multilänkat TETRA nätverk. Det som behandlas är vilken typ av meddelande som bör användas samt hur man väljer väg. De meddelanden som hanteras är klartextmeddelanden, status samt GPS positionsmeddelanden. Den lösning som tagits fram är implementerad som en del i ISIS programvaran (som är utvecklad av Know IT Dataunit). Multilänkdelen kunde inte testas, därför gjordes aldrig någon implementering eller utvärdering. Utvärderingen av implementeringen som hanterar sändning och mottagning av meddelanden visar att den föreslagna lösningen uppfyller de krav man kan ställa på en sådan produkt. Under ett fyra dagar långt test skickades meddelanden (korta textmeddelanden samt positioneringsmeddelanden) medan ett normalt antal röstsamtal pågick, utan någon förlust av paket.

No description available.

Traditionally, and up to this date, Land Mobile Radio (LMR) systems have been interconnected via leased lines and microwave links across circuit-switched networks. With the recent deployment of digital LMR standards such as the Association of Public and Communications Officials (APCO) Project 25 and the Terrestrial Trunked Radio (TETRA), traffic exchange has become more bursty and non-uniform, and as such, less suitable for circuit-switched networks. This thesis proposes a framework for a packet-switched Inter-System Interface (ISI) for LMR systems. Packet-switched networks have the advantage of supporting traffic integration, utilize capacity efficiently, scale easily and seamlessly, and eliminate single points of failure by providing a distributed architecture. Session Initiation Protocol (SIP) signaling messages are defined for setting up and tearing down unit-to-unit calls across the ISI. The Session Description Protocol (SDP) is used to describe how the voice calls are encoded. Voice packets are exchanged between LMR users using the Real-Time Transport Protocol (RTP). Based on the proposed framework, we develop a simulation model to investigate the performance of the ISI when different numbers of LMR users try to establish unit-to-unit calls across the packet-switched ISI. Three packet transport technologies providing Wide Area Network (WAN) connectivity are considered, IP, ATM, and Frame Relay. The results indicate that a packet-switched ISI can take advantage of statistical multiplexing techniques to distribute network resources more efficiently. Quantitative results are obtained for throughput and link utilization. When using an access link providing T1 service, we show that the End-To-End (ETE) delay, and delay variation can be controlled at levels capable of supporting the timely delivery of real-time voice packets. Assuming link utilization is maintained below 100%, the maximum ETE delay experienced in all three packet transport technologies considered is 58 ms and the maximum call setup time is less than 300 ms. An ATM WAN provides the best performance for all time-dependent metrics considered, i.e., ETE delay, delay variation, and call setup time. An IP WAN provides the highest bandwidth efficiency. Selecting the appropriate packet transport technology for the WAN is a tradeoff between the delay that can be tolerated by the voice packets traversing the LMR network and the cost of bandwidth on the access link. / Master of Science

Biomimetic tactile sensing was previously mostly performed in medical situations, such as when locating tumors in patients’ bodies. This thesis examined the effectiveness of using a biomimetic tactile sensory equipment for examining pressure distribution throughout carton board packages, made in two different carton board qualities. The purpose was to examine to what extent biomimetic tactile sensing was able to mimic the results of a group of human test subjects evaluations. Eight packages, made from two different materials, were tested. There were four packages of each of the materials. Each package had four points where displacement measurements with a force of 6N were conducted. The packages were then measured twice on a single point on the edge of the package, with the force of 12N.  The packages at disposal were compressed using a uniaxial-tensile-testing machine alongside with the aforementioned equipment. The pressure sensitive film was placed on top of the packages and a limit on the maximum force to be applied was set on the testing machine. Two limits on the applied forces were set, the first to see the distribution of pressure within the range of elastic deformation, so that no lasting deformation would have occurred. The second force limit was set to see the moment where the elastic deformation area transformed into the plastic deformation area, to see whether or not there was a difference in the distribution of pressure pre- or post-plastic deformation. From the results from compression tests, it was clear that there was a difference in pressure distribution before and after the plastic deformation had occurred. The experimental diagrams showed that the curves were vastly different in both cases. It was also clear that there was a significant difference in the distribution of pressure, depending on if the pressure was applied closer to the middle compared to closer to the center of the package (single vs multiple concentration of forces, respectively). Inspecting results from packages made in both carton board qualities, there were no clear results as the same trends could be seen throughout the tests.  It was concluded that the BioTac could be used to accurately identify concentrations of forces, differences in pressure distribution and the location of deformation. This means that the BioTac will be useful in future experiments, when objectively evaluating and defining grip stiffness, with the help of methods such as the finite-element method. / Biomimetiskt taktilt avkännande var något som tidigare mestadels utfördes för medicinala syften, såsom för att lokalisera tumörer i patienters kroppar. Detta examensarbete undersökte effektiviteten av att använda en biomimetisk taktil avkännare för att granska tryckfördelningen genom kartongförpackningar, gjorda av material från två olika kartongkvaliteter. Totalt åtta förpackningar, gjorda av två olika material, provades. Det var fyra av varje materialtyp. Varje förpackning mättes på fyra punkter med 6N och sedan två gånger på samma punkt med 12N. Förpackningarna till förfogande trycktes ihop med en enaxlad drag- och tryckprovare samt den tidigare nämnda avkännaren. En tryckkänslig film användes mellan avkännaren och förpackningen för att tydligt se tryckfördelningen på alla förpackningarna. Två gränser på den maximala tillåtna kraften upprättades i tryckmaskinen, 6N och 12N. Den lägre nivån sattes för att undersöka tryckfördelningen inom det elastiska deformationsområdet, utan att en kvarstående plastisk deformation uppstått. Den högre gränsen sattes för att undersöka skedet där det elastiska deformationsområdet övergår till det plastiska. Båda gränsvärdena valdes för att undersöka om det gick att urskilja en skillnad i tryckfördelningen innan och efter plastisk deformation, eller inte.  Från resultaten av dessa kompressionstest var det tydligt att det fanns en skillnad i tryckfördelning före och efter den plastiska deformationen uppstått. De uppställda diagrammen visade att kurvorna var mycket annorlunda i båda fallen. Det var, dessutom, relativt tydligt att det fanns en skillnad i tryckfördelning beroende av om trycket var applicerat närmre mitten av paketet jämfört med om det var applicerat närmre kanten av paketet (enskilda kraftkoncentrationer vid mitten av förpackningarna och multipla koncentrationer vid kanten av förpackningarna). Genom att undersöka resultat från förpackningar gjorda av båda kartongkvaliteter, upptäcktes ingen tydlig skillnad i förpackningarna, då liknande trender uppstod i båda materialen. Slutsatsen drogs att BioTac kunde användas för att finna kraftkoncentrationer, skillnader i tryckfördelning, samt området för deformation. Detta betyder att BioTac-sensorn kan vara nyttig vid framtida experiment, för att objektivt utvärdera och definiera greppstyvhet, med metoder som finita-element-metoden.

As reações de metatese assim como o desenvolvimento de complexos metal-carbeno (W, Mo, Ru) se tornaram amplamente conhecidos na química graças aos avanços que trouxeram. Na química orgânica, possibilitou a síntese de macrocíclos por meio da RCM e na química de polímeros a ROMP permitiu um alto grau de controle da polimerização além da alta reatividade dos catalisadores. Isso fez desta reação uma poderosa ferramenta no desenvolvimento de novos materias. A tentativa de combinar a processabilidade dos polímeros com as propriedades luminescentes de determinandos compostos tem sido tema de muitas pesquisas.O objetivo deste trabalho é derivatizar compostos luminescentes , como o complexo metálico [Ru(bpy)3] (PF6)2e a porfirina tetrakis-(pentafluorfenil)porfirina (TPPF20), com o monômero norborneno (NBE) para obtenção de monômeros lumiscentes passíveis de serem polimerizados via ROMP. Aqui será apresentado e discutido a síntese e a caracterização desses compostos partindo-se dos reagentes: ácido-5-norborneno-2carboxílico; etilenodiamina e o ácido-2,2\'-bipiridina-4,4\'-dicarboxílico, cis-[RuCl2(bpy)2] e a porfirina TPPF20. Os compostos foram sintetizados com rendimentos superiores a 60%, com exceção da obtenção da porfirina, e caracterizados por RMN - 1H e 13C; FTIR; UV-vis. Os estudos de copolimerização entre NEN (norborneno-etilenodiamina-norborneno), NBE-TPPF e [Ru(bpy)2(NBbpy)](PF6)2 foram feitos usando o catalisador de Grubbs segunda gereação. Os polímeros gerados foram caracterizados usando TG-FTIR e RMN-1H, as massas moleculares não puderam ser calculadas por GPC porém os estudos indicam obtenção de baixo peso molelcular. O material obtido foi testado como sensibilizador em vidro condutor ITO, porém não houve adsorção do composto necessitam algumas modificações no metalomonômero. / The metathesis reactions as well as the development of metal-carbene complexes (W, Mo, Ru) became widely known in chemistry thanks to advancements brought. In organic chemistry, it enabled the synthesis of macrocycle by RCM. Useful for the synthesis of natural products. In polymer chemistry, ROMP, allow a high degree of polymerization control. This made the ROMP reaction a powerful tool in the development of new materials. In attempt to combine the processability of polymers with luminescent properties of such compounds has been the subject of several researchs. The aim of this work is to synthesize luminescent compounds based on [Ru(bpy)3] (PF6)2 metal complex and on the porphyrin tetrakis-(pentafluorphenyl)porphyrin (TPPF20), funcionalized with pendant norbornene monomer (NBE) to obtain lumiscentes monomers which can undergo ROMP polymerization. Here will be discussed the synthesis and characterization of these compounds using as starting materials: 5-norbornene-2-carboxylic acid; ethylenediamine; 2,2\'-bipyridine-4,4\'dicarboxylic acid, cis-[RuCl2(bpy)2] and the porphyrin TPPF20. The compounds were synthesized with yields higher than 60%, with the exception of the porphyrin, and characterized by 1H and 13C-NMR; FTIR; UV-vis. Copolymerization reaction between NEN (Norbornene-Ethylenediamine-Norbornene), NBE-TPPFe [Ru(bpy)2(NBbpy)] (PF6)2 were performed using Grubbs second generation catalyst. Resultinf polymers were characterized by TG-FTIR and 1H-NMR, molecular weight could not be measured by GPC, since polymers were insoluble, but studies indicate low molelcular weight. The material obtained was tested as sensitizer in conductive glass ITO. The polymer didn\'t adsorb in to the ITO surface. Some changes in the compound molecular structure are required to improve adsorption.

As reações de metatese assim como o desenvolvimento de complexos metal-carbeno (W, Mo, Ru) se tornaram amplamente conhecidos na química graças aos avanços que trouxeram. Na química orgânica, possibilitou a síntese de macrocíclos por meio da RCM e na química de polímeros a ROMP permitiu um alto grau de controle da polimerização além da alta reatividade dos catalisadores. Isso fez desta reação uma poderosa ferramenta no desenvolvimento de novos materias. A tentativa de combinar a processabilidade dos polímeros com as propriedades luminescentes de determinandos compostos tem sido tema de muitas pesquisas.O objetivo deste trabalho é derivatizar compostos luminescentes , como o complexo metálico [Ru(bpy)3] (PF6)2e a porfirina tetrakis-(pentafluorfenil)porfirina (TPPF20), com o monômero norborneno (NBE) para obtenção de monômeros lumiscentes passíveis de serem polimerizados via ROMP. Aqui será apresentado e discutido a síntese e a caracterização desses compostos partindo-se dos reagentes: ácido-5-norborneno-2carboxílico; etilenodiamina e o ácido-2,2\'-bipiridina-4,4\'-dicarboxílico, cis-[RuCl2(bpy)2] e a porfirina TPPF20. Os compostos foram sintetizados com rendimentos superiores a 60%, com exceção da obtenção da porfirina, e caracterizados por RMN - 1H e 13C; FTIR; UV-vis. Os estudos de copolimerização entre NEN (norborneno-etilenodiamina-norborneno), NBE-TPPF e [Ru(bpy)2(NBbpy)](PF6)2 foram feitos usando o catalisador de Grubbs segunda gereação. Os polímeros gerados foram caracterizados usando TG-FTIR e RMN-1H, as massas moleculares não puderam ser calculadas por GPC porém os estudos indicam obtenção de baixo peso molelcular. O material obtido foi testado como sensibilizador em vidro condutor ITO, porém não houve adsorção do composto necessitam algumas modificações no metalomonômero. / The metathesis reactions as well as the development of metal-carbene complexes (W, Mo, Ru) became widely known in chemistry thanks to advancements brought. In organic chemistry, it enabled the synthesis of macrocycle by RCM. Useful for the synthesis of natural products. In polymer chemistry, ROMP, allow a high degree of polymerization control. This made the ROMP reaction a powerful tool in the development of new materials. In attempt to combine the processability of polymers with luminescent properties of such compounds has been the subject of several researchs. The aim of this work is to synthesize luminescent compounds based on [Ru(bpy)3] (PF6)2 metal complex and on the porphyrin tetrakis-(pentafluorphenyl)porphyrin (TPPF20), funcionalized with pendant norbornene monomer (NBE) to obtain lumiscentes monomers which can undergo ROMP polymerization. Here will be discussed the synthesis and characterization of these compounds using as starting materials: 5-norbornene-2-carboxylic acid; ethylenediamine; 2,2\'-bipyridine-4,4\'dicarboxylic acid, cis-[RuCl2(bpy)2] and the porphyrin TPPF20. The compounds were synthesized with yields higher than 60%, with the exception of the porphyrin, and characterized by 1H and 13C-NMR; FTIR; UV-vis. Copolymerization reaction between NEN (Norbornene-Ethylenediamine-Norbornene), NBE-TPPFe [Ru(bpy)2(NBbpy)] (PF6)2 were performed using Grubbs second generation catalyst. Resultinf polymers were characterized by TG-FTIR and 1H-NMR, molecular weight could not be measured by GPC, since polymers were insoluble, but studies indicate low molelcular weight. The material obtained was tested as sensitizer in conductive glass ITO. The polymer didn\'t adsorb in to the ITO surface. Some changes in the compound molecular structure are required to improve adsorption.

Hydrogen is currently considered by many as the “fuel of the future”. It is particularly favoured as a replacement for fossil fuels due to its clean-burning properties; the waste product of combustion being water. While hydrogen is relatively easy to produce, there is currently a lack of practical storage methods for molecular H2, and this is greatly hindering the use of hydrogen as a fuel. Gases are normally stored in vessels under only moderate pressures and in liquid form where possible, which yields the highest energy density. However, to store reasonable quantities of hydrogen in similar volume containers, cryogenic temperatures or extreme pressure are required. Many potential hydrogen storage technologies are currently under investigation, including adsorption on metal hydrides, nanotubes and glass microspheres, and the chemical breakdown of compounds containing hydrogen to release H2. Recent studies have sparked interest in hydrates as a potential hydrogen storage material. The molecular storage of hydrogen in clathrate hydrates could offer significant benefits with regard to ease of formation/regeneration, cost and safety, as compared to other storage materials currently under investigation. Here, we present new experimental hydrate stability data for sII forming hydrogen–water (up to pressures of 180 MPa) and hydrogen–water–tetrahydrofuran systems, the structure-H forming hydrogen–water–methyclycohexane system, and semi-clathrate forming hydrogen–water–tetra-n-butyl ammonium bromide/tetra–n-butyl ammonium fluoride systems.

Student Number : 0009404H - PhD thesis - School of Chemistry - Faculty of Science / Six-coordinate ruthenium complexes of the type ttt-RuX2(RNC)2(PPh3)2 (X = Cl ; R = tBu, 2,6-xylyl, benzyl, 2-OMe-4-Clphenyl and iPr; X = Br; R = = tBu, 2,6-xylyl, benzyl, iPr ) were synthesized and fully characterised by IR, NMR spectroscopy and elemental analysis. These complexes were all found to undergo solid-state isomerisation from the ttt-isomer to the cct-isomer. It was found that solid state isomerisation occurred before melting i.e. in the solid state for all the ttt-RuX2(RNC)2(PPh3)2 complexes investigated . The thermal solid state isomerisation process was monitored by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), thermomicroscopy and X-ray powder diffraction techniques. The results revealed that the isomerisation process occurred exothermally with darkening of the crystal. A mechanism which involved rotation of small ligands was proposed to explain the solid-state isomerisation process. Kinetic studies of the solid-state isomerisation of some of the ttt-RuCl2(RNC)2(PPh3)2 complexes were carried out and monitored by NMR spectroscopy and compared with kinetic studies performed by XRD methods. The results are similar and also suggest that the isomerisation reaction follows first order kinetics. The crystal and molecular structure of ttt-RuCl2(2,6-xylylNC)2(PPh3)2 was determined by X-ray single crystal diffraction techniques and its structure was compared with related ttt- RuX2(RNC)2(PPh3)2 complexes. Inter-molecular and intra-molecular interactions in these molecules in the solid state were analysed and used to rationalise the solid-state isomerisation results. The results obtained for the ruthenium complexes suggested that other six-coordinate complexes could undergo solid state isomerisation reactions and this study was extended to molybdenum complexes of the type cis-Mo(CO)4L2. These complexes undergo cis to trans isomerisation in solution. The solid state reactivity of these complexes was investigated for the first time. The complexes with L = PPh2Me and PPh2Et isomerised in the melt whist complexes with L = PPh3 amd P(m-tolyl)3 were indeed found to undergo solid state isomerisation reactions. Structural studies and thermomicroscopic investigations were done on cis- Mo(CO)4(PPh3)2 and Mo(CO)4{P(m-tolyl)3)2 and the results were used to rationalize the solid state reactivity of these complexes.

O Glioblastoma é um dos tumores mais agressivos do sistema nervoso central e entre as diversas neoplasias possui um dos piores prognósticos. Mesmo com as novas estratégias de tratamento, a sobrevida de pacientes portadores de glioblastoma continua sendo muito baixa, sendo a temozolomida (TMZ) o agente mais comum usado no seu tratamento. O ácido tetra-o-metil nordihidroguaiarético (M4N), é um novo agente terapêutico que funciona como um repressor transcricional global de genes dependentes do fator de transcrição Sp1, tais como Survivina e Cdk1. No presente estudo, foram investigados os níveis de expressão do gene Survivina, suas variantes gênicas por splicing alternativo e Cdk1 em amostras tumorais e linhagens celulares de GBM. Adicionalmente, foram investigados os efeitos do M4N em combinação ou não com TMZ e/ou radiação em culturas primárias e linhagens celulares de GBM. Ensaios de qRT-PCR foram realizados para determiner a expressão de mRNA das variantes gênicas de Survivina e Cdk1. A proliferação celular foi analisada pelo ensaio XTT e os niveis de apoptose e variações do ciclo celular foram determinados por citometría de fluxo. Analises de combinação de drogas utilizando diferentes estratégias de administração (simultânea e seqüencial) foram realizados baseados no método de Chou-Talalay em linhagens celulares e culturas primárias de GBM. Para os ensaios de sobrevivência clonogênica, foram utilizadas as doses de 2, 4 e 6 Gy de radiação gamma. Todas as variantes por splicing alternativo de Survivina e o gene Cdk1 foram expressos em amostras (n=16) e linhagens celulares (n=6) de GBM, exceto a variante Survivina-2B que apenas foi expressa nas linhagens celulares de GBM. O tratamento com M4N diminuiu a expressão de Cdk1, Survivina e a variante Survivina-Ex3, enquanto que houve um aumento da expressão da variante Survivina-2B. O M4N diminuiu a proliferação celular de forma isolada e sinérgicamente quando combinada com TMZ. Além disso, o M4N aumentou os efeitos da radiação, principalmente quando associado com TMZ. O M4N causou morte celular apoptótica, diminuição do índice mitótico e parada do ciclo celular principalmente na fase x G2/M. Os resultados do presente estudo sugerem a potencial aplicação clínica de M4N em combinação com TMZ e radiação no tratamento do GBM. / Glioblastoma (GBM), one of the most human malignant neoplasia, responds poorly to current treatment modalities, being temozolomide (TMZ) the most used drug in its treatment. TetraO-methyl Nordihydroguaiaretic Acid (M4N) is a global transcriptional repressor of genes dependents of Sp1 transcription factor, such as Survivin and Cdk1. In this study was evaluated the gene expression of Survivin, their spliced-variants and Cdk1 in GBM samples and cell lines. Moreover, it was investigated the effects of M4N combined or not with TMZ and/or radiation on primary cultures and cell lines of GBM. qRT-PCR assays were performed to determine the Survivin-spliced variants and Cdk1 gene mRNA expression in GBM tumor samples and cell lines. Cell proliferation was measured by XTT assay and cell cycle and apoptosis were determined by flow cytometry. Drug combination analyzes using different schedules of administration (simultaneous and sequential) were performed based in ChouTalalay method on GBM cell lines and primary cultures. For clonogenic survival, it was used the doses of 2, 4, and 6 Gy of gamma radiation. All Survivin-spliced variants and Cdk1 gene were expressed in GBM samples (n=16) and cell lines (n=6), except the Survivin-2B variant that was only expressed in GBM cell lines. M4N treatment down regulated the expression of Cdk1, Survivin and Survivin-Ex3 variant, while the Survivin-2B variant was up-regulated. M4N decreased the cell proliferation separately and synergistically with TMZ, moreover it enhanced the radiation effects, mainly when associated with TMZ. M4N also induced apoptotic cell death, decreased mitotic index and arrested the cell cycle mainly in G2/M phase. Our results suggest a potential clinical application of M4N in combination with TMZ and radiation in GB treatment.

<p>Enligt rådets förordning 4064/89, om kontroll av företagskoncentrationer, är det kommissionen som kontrollerar och granskar företagskoncentrationer. Den så kallade ”one stop shop-principen” innebär att företag som genomför en fusion med gemenskapsdimension endast ska behöva vända sig till en instans, det vill säga kommissionen för att få en bedömning avseende fusionens förenlighet med den gemensamma marknaden. Eftersom kommissionen är ensam beslutsinstans är det därmed också kommissionen som har fått utforma standarden för vilken bevisning och vilka antaganden som anses tillräckliga för att bevisa att en skadlig företagskoncentration föreligger. Förstainstansrätten har sedan den installerades 1989 haft funktionen att handlägga överklaganden av kommissionens beslut i konkurrensärenden. Fram tills nyligen har förstainstansrätten endast kommenterat formella felaktigheter i kommissionens beslut. I tre nya avgöranden, Airtours plc mot kommissionen, Schneider Electric SA mot kommissionen samt Tetra Laval BV mot kommissionen, har förstainstansrätten emellertid upphävt kommissionens beslut med anledning av materiella felaktigheter. Förstainstansrätten har underkänt kommissionens bevisning och slutsatsdragande och konstaterat att någon skadlig företagskoncentration inte var förhanden i något av de tre fallen. Det ovan beskrivna kan tolkas som att det finns en uppfattning från förstainstansrättens sida att kommissionen har varit för skönsmässig i sina bedömningar och att en standard måste fastställas när det gäller kontrollen av företagskoncentrationer. Problemformulering Har förstainstansrätten lagt grunden för nya standarder när det gäller kraven för vad som är godtagbar bevisning och godtagbart slutsatsdragande i mål om företagsfusioner?</p>