<p>It is difficult for designers to explain what they do. In addition,those unfamiliar with design do not understand the rigor and logic of design thinking and process .We can’t formulate pre defined model for design process because every design situation is unique and new situation. But I tried to formulate my own model for design process as common with Inspirations from my supervisors/Lectures. I tried to describe what is design? And what is design process? In this paper.This personal position paper explores the personal improvement throughout the course work and what I learnt though out the course work.</p>

<p>Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. These reporter molecules can be designed to include tag sequences that can be analyzed by techniques for nucleic acid analysis. Herein, I present a dual-tag microarray (DTM) platform that is suitable for high-performance analyses of DNA reporter molecule libraries, generated by padlock and proximity probing reactions. The DTM platform was applied for analysis of mRNA transcripts using padlock probes, and of cytokines using proximity ligation. The platform drastically improved specificity of detection, and it allowed precise measurements of proteins and nucleic acids over wide dynamic ranges.</p><p>The thesis also presents two techniques for multi-probe analyses of biomolecules: the triple-specific proximity ligation assay (3PLA) for protein analyses, and the spliceotyping assay for mRNA analyses. 3PLA allows highly specific measurements of as little as hundreds of target protein molecules by interrogating three target epitopes simultaneously. In spliceotyping the exon composition of individual transcripts are represented as a series of tag sequences in DNA reporter molecules, via a series of target-dependent ligation reactions. Next, the splicing patterns along individual transcripts can be revealed by amplified single molecule detection and step-wise decoding.</p>

Geometric Circle Packings are of interest not only for their aesthetic appeal but also their relation to discrete analytic function theory. This thesis presents new computational methods which enable additional practical applications for circle packing geometry along with providing a new discrete analytic interpretation of the classical Schwarzian derivative and traditional univalence criterion of classical analytic function theory. To this end I present a new method of computing the maximal packing and solving the circle packing layout problem for a simplicial 2-complex along with additional geometric variants and applications. This thesis also presents a geometric discrete Schwarzian quantity whose value is associated with the classical Schwarzian derivative. Following Hille, I present a characterization of circle packings as the ratio of two linearly independent solutions of a discrete difference equation taking the discrete Schwarzian as a parameter. This characterization then gives a discrete interpretation of the classical univalence criterion of Nehari in the circle packing setting.

A new integrated layout optimization method is proposed here for the design of multi-component systems. By introducing movable components into the design domain, the components layout and the supporting structural topology are optimized simultaneously. The developed design procedure mainly consists of three parts: (i). Introduction of non-overlap constraints between components. The Finite Circle Method (FCM) is used to avoid the components overlaps and also overlaps between components and the design domain boundaries. It proceeds by approximating geometries of components and the design domain with numbers of circles. The distance constraints between the circles of different components are then imposed as non-overlap constraints. (ii). Layout optimization of the components and supporting structure. Locations and orientations of the components are assumed as geometrical design variables for the optimal placement. Topology design variables of the supporting structure are defined by the density points. Meanwhile, embedded meshing techniques are developed to take into account the finite element mesh change caused by the component movements. Moreover, to account for the complicated requirements from aerospace structural system designs, design-dependent loads related to the inertial load or the structural self-weight and the design constraint related to the system gravity center position are taken into account in the problem formulation. (iii). Consistent material interpolation scheme between element stiffness and inertial load. The common SIMP material interpolation model is improved to avoid the singularity of localized deformation due to the presence of design dependent loading when the element stiffness and the involved inertial load are weakened with the element material removal. Finally, to validate the proposed design procedure, a variety of multi-component system layout design problems are tested and solved on account of inertia loads and gravity center position constraint.

This thesis describes a new approach to biomolecular analysis, called the volume-amplified magnetic nanobead detection assay (VAM-DNA). It is a sensitive, specific magnetic bioassay that offers a potential platform for the development of low-cost, easy-to-use diagnostic devices. The VAM-NDA consists of three basic steps: biomolecular target recognition, enzymatic amplification of the probe-target complex using the rolling circle amplification (RCA) technique, and addition of target complementary probe-tagged magnetic nanobeads which exhibit Brownian relaxation behavior. Target detection is demonstrated by measuring the frequency-dependent complex magnetization of the magnetic beads. The binding of the RCA products (target DNA-sequence coils) to the bead surface causes a dramatic increase in the bead size, corresponding essentially to the size of the DNA coil (typically around one micrometer). This causes a decrease in the Brownian relaxation frequency, since it is inversely proportional to the hydrodynamic size of the beads. The concentration of the DNA coils is monitored by measuring the decrease in amplitude of the Brownian relaxation peaks of free beads. The parameters oligonucleotide surface coverage, bead concentration, bead size and RCA times were investigated in this thesis to characterize features of the assay. It was found that all of these parameters affect the outcome and efficiency of the assay. The possibility of implementing the assay on a portable, highly sensitive AC susceptometer platform was also investigated. The performance of the assay under these circumstances was compared with that using a superconducting quantum interference device (SQUID); the sensitivity of the assay was similar for both platforms. It is concluded that, the VAM-NDA opens up the possibility to perform biomolecular detection in point-of-care and outpatient settings on portable platforms similar to the one tested in this thesis. Finally, the VAM-NDA was used to detect Escherichia coli bacteria and the spores of Bacillus globigii, the non-pathogenic simulant of Bacillus anthracis. A limit of detection of at least 50 bacteria or spores was achieved. This shows that the assay has great potential for sensitive detection of biomolecules in both environmental and biomedical applications.

Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. These reporter molecules can be designed to include tag sequences that can be analyzed by techniques for nucleic acid analysis. Herein, I present a dual-tag microarray (DTM) platform that is suitable for high-performance analyses of DNA reporter molecule libraries, generated by padlock and proximity probing reactions. The DTM platform was applied for analysis of mRNA transcripts using padlock probes, and of cytokines using proximity ligation. The platform drastically improved specificity of detection, and it allowed precise measurements of proteins and nucleic acids over wide dynamic ranges. The thesis also presents two techniques for multi-probe analyses of biomolecules: the triple-specific proximity ligation assay (3PLA) for protein analyses, and the spliceotyping assay for mRNA analyses. 3PLA allows highly specific measurements of as little as hundreds of target protein molecules by interrogating three target epitopes simultaneously. In spliceotyping the exon composition of individual transcripts are represented as a series of tag sequences in DNA reporter molecules, via a series of target-dependent ligation reactions. Next, the splicing patterns along individual transcripts can be revealed by amplified single molecule detection and step-wise decoding.

It is difficult for designers to explain what they do. In addition,those unfamiliar with design do not understand the rigor and logic of design thinking and process .We can’t formulate pre defined model for design process because every design situation is unique and new situation. But I tried to formulate my own model for design process as common with Inspirations from my supervisors/Lectures. I tried to describe what is design? And what is design process? In this paper.This personal position paper explores the personal improvement throughout the course work and what I learnt though out the course work.

Det är en betydande andel svenska resenärer som idag reser till och runt i Thailand som ”backpackers”. Mitt syfte är att ta reda på om den svenska backpackerdominansen i Thailand skulle kunna betecknas som en kolonisering. Material och metod består av intervjuer och deltagande observation med sju informanter, samt fältundersökningar som bedrivits i Thailand. Slutresultatet tolkas med hjälp av den postkoloniala teoribildningen. Frågeställningarna koncentreras kring interaktionen mellan svenska backpackers och lokala subjekt, återvändandets betydelse, samt om en modern kolonisering i enlighet med Victor Mudimbes terminologi kan appliceras. Jag kommer fram till att svenska backpackers i Thailand visar tendenser och tillämpningar som tyder på en modern kolonisering av platserna de befinner sig på.

The in situ proximity ligation assay (in situ PLA) is an immunoassay that enables directvisualisation of single protein targets or protein interactions in cell or tissue samples. This project revolves around designing and introducing several novel multiplexable components tobe used in conjunction with Olink Bioscience's Duolink product line. In this report, a novel in silico approach to DNA oligomer interaction design is presented. Using this in silico method, a multiplexed system of DNA oligomers has been designed andevaluated using in situ PLA and fluorescence microscopy.

Förskolans läroplan (Lpfö 98) säger att barnet i förskolan ska få en grundläggande förståelse för demokratins innebörd. Den pedagogiska verksamhetens utformning ska möjliggöra att barnet kan ge uttryck för sina intressen och påverka sin situation. Den här studiens syfte är att undersöka hur barnets delaktighet framträder i förskolans verksamhet. Som avgränsat undersökningsområde har förskolans pedagogiska samling valts. Studien har genomförts med en kvalitativ metod. För insamlandet av det empiriska materialet gjordes observationer under samlingstillfällena på en förskoleavdelning med barn i åldrarna fyra till sex år. Det insamlade materialet analyserades med utgångspunkt i ett sociokulturellt perspektiv. Under analysen framkom att barnen uttryckte sina intressen genom verbalt språk, kroppsspråk och handlingar. Barnen gavs möjligheter till delaktighet och inflytande genom att förskollärarna tog hänsyn till deras intressen, värnade om deras aktiva deltagande och gav dem valmöjligheter. Det som barnen genom inflytandet påverkade var samlingens innehåll, dess tidslängd och hur engagemanget i barngruppen blev. Diskussionen behandlar studiens resultat och tidigare forskning. Den belyser vilka konsekvenser barnens uttrycksätt kan få för alla barn under samlingen. Även barnens inflytande på varandra och pedagogens betydelse diskuteras. Studiens slutsats är att barnen i viss mån har inflytande i förskolans samling. Graden av inflytande varierar beroende på barnets förmåga att uttrycka sina intressen och pedagogens uppmärksammande och tillvaratagande av det barnen ger uttryck för. / The Swedish preschool curriculum (Lpfö 98) says that the child in preschool should have a basic understanding of democracy. The educational activity should allow the child to express its own interests and to have a direct influence on the daily activities in preschool. This study aims to examine how children’s participation appears in preschool. Among the daily activities in preschool is circle time. This activity has been chosen as defined study area. The study was conducted with a qualitative approach. For the collection of the empiric material observations were made during the circle time and with children aged four to six years. The collected material was analyzed on the basis of a sociocultural perspective. The analysis revealed that the children expressed their interests through verbal language, body language and actions. The children were given the possibility to participate and to use their influence because the preschool teachers took into account their interests, guarded their active participation and gave them choices. The children influenced the contents and the duration of the circle time as well as the atmosphere in the group. The discussion deals with the study results and previous research and highlights the ways of expression of the children. The children's influence on each other and the importance of the teacher is also discussed. The study concludes that, to some extent, preschool children have an influence on the circle time activity. The degree of influence varies depending on the child's ability to express its interests and the teacher attention and procurement of the children’s influence.