Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T13:19:50Z No. of bitstreams: 1 TES_VIRGINIA_CARLA_DE_ALMEIDA_FALCAO_COMPLETO.pdf: 3588046 bytes, checksum: 337be6be89fea4ea58787c070a072a51 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T13:19:59Z (GMT) No. of bitstreams: 1 TES_VIRGINIA_CARLA_DE_ALMEIDA_FALCAO_COMPLETO.pdf: 3588046 bytes, checksum: 337be6be89fea4ea58787c070a072a51 (MD5) / Made available in DSpace on 2017-06-30T13:20:08Z (GMT). No. of bitstreams: 1 TES_VIRGINIA_CARLA_DE_ALMEIDA_FALCAO_COMPLETO.pdf: 3588046 bytes, checksum: 337be6be89fea4ea58787c070a072a51 (MD5) Previous issue date: 2017-03-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul (FAPERGS) / Tuberculosis (TB) has become the leading global cause of death from infectious diseases. In 2015, according to WHO, 10.4 million new cases of tuberculosis worldwide have emerged. Currently the commonly used treatments are not effective against the forms of disease resistant to the most effective anti-TB drugs, and drugs with new mechanisms of action are needed. Mycobacterium tuberculosis dihydroneopterin aldolase (MtDHNA /FolB) is a folate enzyme encoded by the folB gene, which has important properties that make it a potential target for the synthesis of new antimicrobial agents. As a first step for target validation in the antimicrobial drug development pipeline, it is important to prove that the gene encoding a putative target is essential for pathogen?s viability. In this study, using site directed mutagenesis, biochemical analyzes and gene knockout experiments, we demonstrated that the folB gene is essential for the survival of Mtb, and furthermore we prove that this essentiality depends on the aldolase/epimerase activities of the MtFolB protein. The wild-type gene (wt) and the point mutants K99A and Y54F were cloned and expressed, and the corresponding recombinant proteins were purified and monitored for the activities of aldolase, epimerase and oxygenase using HPLC. In contrast to the wild-type MtFolB (wt) enzyme, both mutants had neither aldolase nor epimerase activities under the conditions tested. The Y54F mutant maintained oxygenase activity, whereas for the K99A mutant it was possible to detect oxygenase activity only in the presence of HP and GA as substrates. Knockout experiments showed that the folB gene is essential for the survival of Mtb under the conditions tested. However, unlike the wild-type copy, when the sequences encoding the K99A or Y54F mutants were used for complementation, no viable colonies were obtained, indicating that these point mutants could not rescue the cells after the folB knockout. These results indicate that aldolase and/or epimerase activities are crucial for the survival of Mtb. The construction of Mycobacterium tuberculosis folB-GFP fusion (Mtb) strains containing wild-type folB gene sequence or a deleted C-terminal mutant (folB?C), devoid of the sequence presumably necessary for anchoring the enzyme within nanocage compartments, were performed and together with other cell biology methods described in this work will be used for a better understanding of MtDHNA/FolB cellular functions and for the validation of this enzyme as a therapeutic target. / A tuberculose (TB) tornou-se a principal causa mundial de morte por doen?as infecciosas. Em 2015, de acordo com a OMS, surgiram 10,4 milh?es de novos casos de tuberculose no mundo. Atualmente os tratamentos comumente utilizados n?o s?o eficientes contra as formas da doen?a resistentes aos f?rmacos anti-TB mais eficazes, sendo necess?rios f?rmacos com novos mecanismos de a??o. A di-hidroneopterina aldolase de Mycobacterium tuberculosis (MtDHNA/FolB) ? uma enzima da via do folato, codificada pelo gene folB, que apresenta caracter?sticas importantes que a tornam um potencial alvo para s?ntese de novos agentes antimicrobianos. Neste estudo, por meio de mutag?nese s?tio-direcionada, an?lises bioqu?micas e experimentos de nocaute g?nico, demostramos que o gene folB ? essencial para a sobreviv?ncia de Mtb, e al?m disso provamos que essa essencialidade depende das atividades de aldolase/epimerase da prote?na MtFolB. O gene do tipo selvagem (wt) e os mutantes pontuais K99A e Y54F foram clonados e expressos, e as prote?nas recombinantes correspondentes foram purificadas e monitoradas para as atividades de aldolase, epimerase e oxigenase utilizando HPLC. Em contraste com a enzima MtFolB selvagem (wt), ambas as mutantes n?o apresentaram atividade de aldolase nem de epimerase nas condi??es testadas. A mutante Y54F manteve a atividade da oxigenase, enquanto que para a mutante K99A foi poss?vel detectar a atividade de oxigenase apenas na presen?a de HP e GA como substratos. Os experimentos de nocaute mostraram que o gene folB ? essencial para a sobreviv?ncia de Mtb sob as condi??es testadas. Entretanto, diferentemente da c?pia selvagem, quando as sequ?ncias que codificam os mutantes K99A ou Y54F foram utilizadas para complementa??o, n?o foram obtidas col?nias vi?veis, indicando que estes mutantes pontuais n?o poderiam resgatar as c?lulas ap?s o nocaute do gene folB. Esses resultados indicam que as atividades de aldolase e/ou epimerase s?o cruciais para a sobreviv?ncia de Mtb. A constru??o de cepas com fus?o folB-GFP de Mycobacterium tuberculosis (Mtb) que cont?m a sequ?ncia do tipo selvagem do gene folB ou um mutante com o C-terminal deletado (folB?C), desprovida da sequ?ncia supostamente necess?ria para a ancoragem da enzima dentro dos compartimentos de nanocargas, foram realizadas e juntamente com outros m?todos de biologia celular descritos neste trabalho tamb?m poder?o ser utilizados para um melhor entendimento das fun??es celulares apresentadas por MtDHNA/FolB e para valida??o dessa enzima como potencial alvo terap?utico.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-06-27T13:42:26Z No. of bitstreams: 1 DEBORAH_STONA_TES.pdf: 479572 bytes, checksum: 09946538cb3d70a32dd07508ec13bcce (MD5) / Rejected by Sheila Dias (sheila.dias@pucrs.br), reason: Trabalho devolvido devido constar no sum?rio o t?pico "Hip?teses", mas no corpo do trabalho n?o aparece, do t?pico "Objetivos" passa para "Materiais e m?todos" no arquivo PDF. on 2018-07-04T12:49:48Z (GMT) / Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-07-19T10:52:46Z No. of bitstreams: 1 DEBORAH_STONA_TES.pdf: 842939 bytes, checksum: 8b8eda0f9181f560f275f85833a4faec (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-07-24T12:53:45Z (GMT) No. of bitstreams: 1 DEBORAH_STONA_TES.pdf: 842939 bytes, checksum: 8b8eda0f9181f560f275f85833a4faec (MD5) / Made available in DSpace on 2018-07-24T13:02:33Z (GMT). No. of bitstreams: 1 DEBORAH_STONA_TES.pdf: 842939 bytes, checksum: 8b8eda0f9181f560f275f85833a4faec (MD5) Previous issue date: 2018-03-26 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Aims: 1) to evaluate the fracture resistance of posterior full-contour crowns of lithium dissilicate, lithium silicate reinforced by zirconia or translucent zirconia cemented on solid abutments; 2) to evaluate if the presence of a zirconia coping can increase the fracture resistance of lithium dissilicate crowns compared to monolithic ceramics made in lithium dissilicate, lithium silicate reinforced by zirconia or translucent zirconia. Materials and methods: Forty RN (regular-neck, Straumann) implant analogs and 4.0 mm solid abutments (Straumann) were embedded in acrylic resin and divided into 5 groups (n = 8): 1) e.max CAD; 2) Celtra Duo; 3) Incoris zirconia TZI; 4) 0.6 mm zirconia coping + e.max CAD; 5) 0.3 mm zirconia coping + e.max CAD. After preparation of the specimens, they were subjected to mechanical fatigue testing with 200N and 500,000 cycles. Following, the samples were submitted to the fracture resistance testing. Results: the means in Newtons (N) were as follows: e.max CAD 210.6Na ? 1447; Celtra Duo 2026Nb ? 355.8; zirconia TZI 2850Nc ? 287.6; 0.6 mm coping + e.max CAD 851Nd ? 108.1; 0.3 mm coping + e.max CAD 980Nd ? 133. Conclusion: monolithic translucent zirconia crowns cemented on solid abutments showed the higher fracture resistance in comparison to lithium dissilicate (e.max CAD) and lithium silicate reinforced by zirconia (Celtra Duo). The use of a zirconia coping associated with lithium dissilicate does not promote superior fracture resistance than monolithic crowns regardless of the thickness of coping and veneering ceramics. / Objetivos: 1) Avaliar a resist?ncia ? fratura de coroas cer?micas de dissilicato de l?tio, silicato de l?tio refor?ada por zirc?nia e zirc?nia transl?cida cimentadas sobre pilares s?lidos; 2) Avaliar se a presen?a de um coping em zirc?nia pode aumentar a resist?ncia ? fratura de coroas de dissilicato de l?tio quando comparadas ?s cer?micas monol?ticas confeccionadas em dissilicato de l?tio, zirc?nia transl?cida ou silicato de l?tio refor?ada por zirc?nia cimentadas sobre pilares s?lidos. Materiais e m?todos: Quarenta an?logos de implantes RN (regular neck, Straumann) e pilares s?lidos 4.0mm (Straumann) foram inclu?dos em resina acr?lica e divididos em 5 grupos (n=8) os quais receberam as seguintes coroas prot?ticas cimentadas: 1) e.max CAD; 2) Celtra Duo; 3) zirc?nia Incoris TZI; 4) coping zirc?nia 0,6mm + e.max CAD; 5) coping zirc?nia 0,3mm + e.max CAD. Ap?s confec??o dos corpos de prova os mesmos foram submetidos ? fadiga mec?nica com carga de 200N e 500.000 ciclos. Na sequ?ncia foram submetidos ao ensaio de carga m?xima de carregamento. Resultados: as m?dias de resist?ncia ? fratura obtidas foram: e.max CAD 1447Na ? 210,6; Celtra Duo 2026Nb ? 355,8; zirc?nia TZI 2850Nc ? 287,6; coping 0,6mm + e.max CAD 851Nd ? 108,1; coping 0,3mm + e.max CAD 980Nd ? 133. Conclus?o: Coroas monol?ticas realizadas em zirc?nia transl?cida cimentadas sobre pilares s?lidos apresentaram a maior resist?ncia ? fratura quando comparadas ao dissilicato de l?tio (e.max CAD) e silicato de l?tio refor?ado por zirc?nia (Celtra Duo). O uso de um coping de zirc?nia associado ao dissilicato de l?tio n?o promove resist?ncia ? fratura superior as coroas monol?ticas independentemente das espessuras do coping e da cer?mica de cobertura.

Made available in DSpace on 2014-12-17T15:38:46Z (GMT). No. of bitstreams: 1 OdaraSF.pdf: 1396799 bytes, checksum: 2e5cb33729ffd1f32ef8505042d653ee (MD5) Previous issue date: 2006-03-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The interest for understanding the relationship of the child with its environment has increased in the whole world during the last few years. Several researchers, using Environmental Psychology as basis, have analyzed the implications of this relation for the child development and the organization of playful spaces. Being a place where children spend a great part of their time and develop many intellectual and social abilities, the school becomes one of the main focus of this research. This study investigated different sectors of the outdoor area of NEI -UFRN, during the recreation time, in which the use of space and the interaction between children were analyzed, through the observation of the child behavior (place-centered and individual-centered mapping). The results had disclosed that the school s outdoor area and its equipments presents a great range of choices possible to the children, however its occupation is not uniform: there are areas very used and others almost without use. Generally, this heterogeneity happens again in relation to the distribution of the interaction states in the sectors, the friendly associative behavior being the most present. The observation of children behaviors favored a better understanding of the use of the spaces, and contributed for discussion about the environment what these users really need for a healthy development, including differences in related to gender, age and daytime. In spite of the studied outdoor areas being vast, pleasant and varied, it needs a better distribution of its equipments and a plan that allows greater children participation in the place organization / O interesse pela compreens?o do relacionamento da crian?a com seu ambiente vem aumentando nos ?ltimos anos, em todo o mundo. Diversos pesquisadores, utilizando a Psicologia Ambiental como base, t?m analisado a import?ncia dessas rela??es para o desenvolvimento infantil e suas implica??es na organiza??o de espa?os l?dicos. Por ser um local onde as crian?as passam grande parte do seu tempo e desenvolvem muitas habilidades intelectuais e sociais, a escola torna-se um dos principais focos dessas pesquisas. Partindo desse pressuposto, investigou-se as diferentes ?reas do p?tio do N?cleo de Educa??o Infantil (NEI), durante o recreio, analisando a utiliza??o do espa?o e os comportamentos de intera??o entre crian?as nesses locais, atrav?s da observa??o do comportamento infantil (mapeamento centrado-no-lugar e centrado-na-pessoa). Os resultados revelaram que a ?rea livre da escola e seus equipamentos permitem grande possibilidade de escolha ?s crian?as, contudo sua ocupa??o n?o ? uniforme, existindo ?reas muito utilizadas e outras quase sem uso. De modo geral, essa heterogeneidade se repete em rela??o ? distribui??o dos estados interacionais pelos setores, sendo o comportamento associativo amig?vel o mais encontrado. A observa??o do comportamento das crian?as n?o s? favoreceu a uma melhor compreens?o da utiliza??o dos espa?os, como tamb?m, contribuiu para a discuss?o sobre os ambientes de que esses usu?rios necessitam para um desenvolvimento saud?vel, at? mesmo no que se refere ? diferen?as em rela??o a g?nero, idade e turnos. Apesar do p?tio estudado, ser grande, agrad?vel e variado, ele necessita de uma melhor distribui??o dos seus equipamentos e de um planejamento que permita maior participa??o das crian?as na organiza??o do lugar

Στην παρούσα εργασία μελετάται η αντίδραση αναγωγής των οξειδίων του αζώτου με προπυλένιο σε ισχυρά οξειδωτικές συνθήκες με χρήση καταλυτών Rh υποστηριγμένων σε τροποποιημένους φορείς TiO2 με ιόντα διαφορετικού σθένους (W6+ ή Ca2+) από το μητρικό κατιόν (Ti4+). Η τροποποίηση του φορέα επιτεύχθηκε με την διάχυση των ενισχυτικών κατιόντων στο κρυσταλλικό πλέγμα του TiO2 σε υψηλές θερμοκρασίες. Στους φορείς που παρασκευάστηκαν πραγματοποιήθηκαν μετρήσεις ειδικής επιφάνειας (ΒΕΤ), κρυσταλλικής δομής (XRD), ειδικής αγωγιμότητας (AC-Impedance) καθώς και μετρήσεις επιφανειακής οξύτητας (TPD-MS). Σε γενικές γραμμές η ενίσχυση του φορέα TiO2 έχει σαν αποτέλεσμα την διατήρηση της ειδικής επιφάνειας, την αύξηση της επιφανειακής οξύτητας, και την αλλαγή της ειδικής αγωγιμότητας σε σχέση με τον μη ενισχυμένο φορέα. Οι υποστηριγμένοι καταλύτες Rh παρασκευάστηκαν με την μέθοδο του υγρού εμποτισμού από νιτρική πρόδρομη ένωση. Οι καταλύτες που παρασκευάστηκαν χαρακτηρίστηκαν ως προς την διασπορά της ενεργού φάσης καθώς και του μέσου μεγέθους των κρυσταλλιτών του μετάλλου με χημορόφηση H2. Η σύγκριση των καταλυτών με βάση την ενεργότητα τους για την αναγωγή του ΝΟ καθώς και την εκλεκτικότητά τους σε Ν2, έδειξε δύο διαφορετικές δράσεις της ενίσχυσης. Ενίσχυση με ιόντα W6+, οδηγεί σε αύξηση της ενεργότητας με παράλληλη ελαφρά αύξηση της εκλεκτικότητας σε Ν2. Η ενίσχυση του καταλύτη με ιόντα Ca2+ οδηγεί σε αύξηση της ενεργότητας για την αναγωγή του ΝΟ με παράλληλη όμως, σημαντική μείωση της εκλεκτικότητας σε Ν2. Η συμπεριφορά αυτή ερμηνεύτηκε με βάση το θεωρητικό υπόβαθρο που περιγράφει τις τροποποιήσεις των ηλεκτρονιακών ιδιοτήτων του φορέα και είναι γνωστό ως φαινομένου DIMSI. Η βελτίωση των καταλυτικών ιδιοτήτων που παρουσιάζουν οι ενισχυμένοι καταλύτες έδωσε το ερέθισμα για την μελέτη του μηχανισμού της αντίδρασης για την αναγωγή του ΝΟ με προπυλένιο στους καταλύτες αυτούς καθώς και τις διαφορές που παρουσιάζουν σε σχέση με τον μη-ενισχυμένο καταλύτη. Η μελέτη του μηχανισμού πραγματοποιήθηκε με χρήση των τεχνικών υπέρυθρης φασματοσκοπίας (FTΙR) και φασματοσκοπίας μάζας (Mass Spectroscopy). Σε γενικές γραμμές τα αποτελέσματα δείχνουν ότι το ΝΟ ροφάται μοριακά σε κέντρα Rh. Παρουσία ανηγμένων κέντρων το ΝΟ διασπάται προς ατομικά ροφημένα είδη Ν και Ο. Ανα-συνδυασμένη εκρόφηση των ειδών Ν οδηγούν στον σχηματισμό Ν2 στην αέρια φάση, ενώ παραγωγή του Ν2Ο σχετίζεται με την δημιουργία του συμπλόκου είδους Rh(NO)2. Η αναγέννηση των ενεργών κέντρων επιτυγχάνεται με την μερική οξείδωση του προπυλενίου σε οξειδωμένα κέντρα Rh αρχικά προς ακρολεϊνη. Περαιτέρω οξείδωση της ακρολεϊνης οδηγεί στον σχηματισμό θραυσμάτων COO- και CHx με τον ενδιάμεσο σχηματισμό ειδών του τύπου του ακρυλικού οξέος. / In the present study, the catalytic reduction of NO with propylene in the presence of excess oxygen was examined, over Rh catalysts supported on doped TiO2 carriers with tungsten or calcium cations. Doped TiO2 carriers were prepared employing the solid state diffusion technique at elevated temperatures. Characterization of doped carriers consisted of specific surface (BET) area and crystalline mode (XRD) measurements as well as measurements of the specific conductivity (AC-Impedance) and surface acidity (TPD-MS). In general, doping TiO2 results in an increase of the surface acidity, in alterations of specific conductivity and in the preservation of the specific surface in a manner, which depends on dopant concentration and calcinations temperature. Supported Rh catalysts were prepared following the wet impregnation method using Rh(NO3)3 as the precursor metal compound. Characterization of the prepared catalysts consisted of dispersion and mean metal crystalline size measurements, employing hydrogen chemisorption at ambient temperature. Comparison of the doped catalysts, as far as the activity toward NO reduction and the selectivity to N2 is concerned, shows two different behaviors. Doping with W6+ cations results in higher NO reduction activity with a slightly increase of selectivity toward dinitrogen formation. Doping with Ca2+ leads to higher catalytic activity for the reduction of NO, while the selectivity was found to decrease upon increasing calcium content. The above observations are explained by the theory of dopant induced metal support interactions (DIMSI). The mechanistic pathways of the above mentioned reaction over Rh/TiO2 catalysts were studied employing infrared (IR) and mass spectroscopy (MS). The results showed, that NO is molecularly absorbed on the Rh. In the presence of reduced Rh sites NO dissociation occurs, resulting in N and O adspecies. Recombination of N species results in the production of dinitrogen in the gas phase. N2O formation is correlated with the presence of the dinitrosyl complex species on the catalytic surface. Reaction of the latter with NO from the gas phase leads to N2O production. Regeneration of oxidized Rh sites is achieved via oxidation of propylene to acrolein. Further oxidation of the latter leads to the formation of carboxylates.

Les travaux de cette thèse concernent l'étude de l'influence des conditions de dépôt sur la microstructure et les propriétés optiques de monocouches dans le but de réaliser des filtres optiques particuliers commercialisables. La méthode de dépôt utilisée est l'évaporation par canon à électrons avec assistance ionique. L'un des deux projets a été de vérifier la faisabilité de filtres optiques infrarouges dans la gamme [2-15] µm avec des matériaux non toxiques. Les monocouches des trois fluorures étudiées ont montré des comportements différents suivant les conditions de dépôt, notamment suivant la température du substrat qui influe fortement sur leur microstructure. L'association du BaF₂ au germanium, avec des réglages optimisés, montre pour différents filtres réalisés des résultats proches de la théorie. Ces filtres sont donc commercialisables. Le second projet a été de développer une machine de dépôt dans le but de pouvoir réaliser des filtres complexes d'une centaine de couches d'épaisseur variable (de 15 à 300 nm) associant des monocouches de TiO₂ à des monocouches de SiO₂. L'étude de l'influence des paramètres de dépôts (pression, assistance ionique, préchauffage du matériau) au travers différentes caractérisations (MEB, AFM, ellipsométrie) a permis une optimisation des réglages de l'enceinte de dépôt plus efficace et adaptée en fonction des spécifications des filtres optiques à réaliser. Puis, une étude ellipsométrique poussée de l'indice de réfraction des monocouches de TiO₂ en fonction de leur épaisseur a conduit à une nette évolution des résultats pour la réalisation du filtre complexe.

Submitted by Emanuel Varela Cardoso (emanuel.varela@ufcg.edu.br) on 2018-06-13T19:56:21Z No. of bitstreams: 1 FRANCISCO PINTO FILHO – TESE (PPGEP) 2016.pdf: 2405186 bytes, checksum: d7b5b1d68600f138fc5134159b39d6fc (MD5) / Made available in DSpace on 2018-06-13T19:56:21Z (GMT). No. of bitstreams: 1 FRANCISCO PINTO FILHO – TESE (PPGEP) 2016.pdf: 2405186 bytes, checksum: d7b5b1d68600f138fc5134159b39d6fc (MD5) Previous issue date: 2016-08-29 / Capes / A busca por inovações tecnológicas nos últimos anos cada vez mais se intensifica. Neste contexto, dois importantes grupos, os lantanídeos e as hidantoínas se destacam com importantes contribuições em diversas áreas de pesquisas apresentando vasto campo de aplicações. Nesses termos, esta pesquisa teve como objetivo sintetizar e caracterizar nanocomplexos dos íons lantanídicos: Eu +3, Er+3 e Nd+3 com 5-(4-metilfenil)-3-fenil-2-tioxo-imidazolidin-4-ona (HPA) e um segundo ligante, 1,10-fenatrolina (Phen), almejando obter complexos com propriedades biológicas. Partindo da reação entre os cloretos dos respectivos lantanídeos com os ligantes orgânicos, sob refluxo de aproximadamente 8 horas e temperatura de 60°C, foram obtidos os complexos de európio, érbio e neodímio. Os nanocomplexos em pó foram caracterizados utilizando técnicas de análises espectroscópicas, térmicas e estrutural. Os complexos de íons lantanídicos apresentaram resultados da análise elementar conforme a estequiometria proposta (1:3:1). A coordenação dos ligantes com os íons lantanídicos ocorreu através dos átomos de oxi gênio e enxofre da estrutura do 5-(4-metilfenil)-3-fenil-2-tioxo-imidazolidin-4-ona (HPA) e dos átomos de nitrogênio (C=N) da 1,10’- fenantrolina, que podem ser observados nos espectros de infravermelho, através dos deslocamentos dessas bandas presentes nos ligantes livres e coordenados. Os espectros UV-Vis apresentaram discretos deslocamentos e mudanças nas intensidades das bandas de absorção dos complexos em relação ao ligante HPA. As curvas TG/DTG do ligante HPA apresentou 3 etapas de decomposição, enquanto, que os complexos apresentaram 4 etapas. O nanocomplexo de Eu+3 apresentou menor estabilidade térmica, o que possibilita o uso destes pós como fármacos. As curvas DSC dos nanocomplexos apresentaram uma série de eventos endotérmicos e exotérmicos correspondentes a processos de decomposição, desidratação, fusão e volatilização que corroboram com as curvas termogravimétricas. Todos os complexos lantanídicos obtidos apresentaram dimensões nanométricas, e o complexo de neodímio apresentou a estrutura mais cristalina. / The search for technological innovations in recent years increasingly intensifies. In this context, two major groups, the lanthanides and hydantoins stand out with important contributions in many areas of research showing wide field of applications. In these terms, this research aims to synthesize and characterize complex of lanthanide ions: Eu+3, Ho+3, Er+3 and Nd+3 with 5-(4-methyl-phenyl)-3-phenyl-2-thioxo-imidazolidin-4-one (HPA) and a second linker, 1,10-fenatrolina (Phen), aiming to obtain complexes with biological properties. From the reaction between the chlorides of lanthanides with the respective organic ligands under reflux for approximately 8 hours and 60°C, were obtained complexes of europium, holmium, erbium and neodymium. The powder complexes were characterized using spectroscopic techniques, thermal and structural analyzes. The complexes of lanthanide ions showed elemental analysis results according to the proposed stoichiometry (1:3:1). The coordination of the ligands with lanthanide ions occurred through oxygen and sulfur atoms in the structure of 5 - (4-methylphenyl) -3-phenyl-2-thioxo-imidazolidin-4-one (HPA) and nitrogen atoms (C = N) of 1,10'- phenanthroline, which can be observed in the infrared spectra, through the displacement of these bands present in free and coordinated ligands. UV-Vis spectra showed discrete displacements and changes in the intensities of the absorption bands of the complex compared to the HPA binder. The TG/ DTG curves HPA binder 3 had decomposition steps, while the complexes showed 4 and 5 steps. The complex synthesized Er+3 showed higher thermal stability. The DSC curves of the complexes showed a series of endothermic and exothermic events corresponding to decomposition processes, dehydration, fusion and volatilization, corroborating the thermogravimetric curves. All lanthanide complexes obtained showed nanometric dimensions, and neodymium complex presented the most crystalline structure.

Made available in DSpace on 2014-12-17T15:14:48Z (GMT). No. of bitstreams: 1 SumaiaSVB.pdf: 841526 bytes, checksum: 44daba23a52822d6285186c75da646a8 (MD5) Previous issue date: 2007-10-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Important advances have been made along the last decade in the study of the lithium behavior in solar-type stars. Among the most important discoveries what attracts attention is that the distribution of lithium abundance in the late F-type giant stars tends to be discontinuous, at the same time of a sudden decline in rotation and a gradual decline according to the temperature for giant red stars of such spectral type. Other studies have also shown that synchronized binary systems with evolved components seem to keep more of their original lithium than the unsynchronized systems. evertheless, the connection between rotation and lithium abundance as well as the role of tidal effects on lithium dilution seem to be more complicated matters, depending on mass, metallicity and age. This work brings an unprecedented study about the behavior of lithium abundance in solartype evolved stars based on an unique sample of 1067 subgiant, giant and supergiant stars, 236 of them presenting spectroscopic binary characteristics, with precise lithium abundance and projected rotational speed. Now the lithium-rotation connection for single and binary evolved stars is analyzed taking into account the role of mass and stellar age / Importantes avan?os foram feitos ao longo da d?cada passada no estudo do comportamento do l?tio em estrelas do tipo solar. Entre as descobertas mais importantes pode-se salientar a tend?ncia para uma descontinuidade na distribui??o da abund?ncia do l?tio em estrelas gigantes do tipo F tardio, paralelamente a um r?pido decl?nio na rota??o e um decl?nio gradual em fun??o da temperatura para as estrelas gigantes vermelhas de tipos espectrais F, G e K. Diferentes estudos tamb?m mostraram que os sistemas bin?rios sincronizados com componentes evolu?das parecem reter mais de seu l?tio original do que sistemas n?o sincronizados. No entanto, a conex?o entre a rota??o e a abund?ncia do l?tio, bem como a fun??o do efeito de mar? na dilui??o do l?tio, parecem ser quest?es mais complicadas, dependendo da massa, da metalicidade e da idade. O presente trabalho traz um estudo in?dito sobre o comportamento da abund?ncia do l?tio para estrelas evolu?das do tipo solar, baseado em uma amostra original de 1067 estrelas gigantes, subgigantes e supergigantes, onde 236 delas apresentam caracter?sticas de bin?rias espectrosc?picas, com abund?ncia precisa do l?tio e velocidade rotacional projetada. A conex?o l?tio-rota??o para estrelas evolu?das simples e bin?rias ? agora analisada verificando-se o papel da massa e da idade estelar sobre tal conex?o.

Made available in DSpace on 2014-12-17T15:15:04Z (GMT). No. of bitstreams: 1 BrunoLCM.pdf: 1188835 bytes, checksum: 99a2796bad65a15f2018815f59859e68 (MD5) Previous issue date: 2003-12-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The connection between rotation, CaII emission flux and lithium abundance is analyzed for a sample of subgiant stars, with evolutionary status was determined from the Toulouse-Geneve code and HlPPARCOS trigonometric parallax measurements. We noted that the distribution of rotation and CaII emission flux, as a function of effective temperature, shows a discontinuity located around the same spectral type, F8IV. Stars located blueward of this spectral type, exhibit a large spread of values of rotation and CaII flux, whereas stars redward of F8lV show essentially low ratation anel low CaII flux. The strength of these declines nevertheless, depends on stellar mass. The distribution of lithium abundances also shows a discontinuity, however with behavior a little more complex for subgiants with mass lower than about 1.2 Solar Masses, this decrease is observed later than that in rotation and CaII flux, whereas for masses higher than 1.2 Solar Masses the decrease in lithium abundance is located around the spectral type F8IV. The discrepancy between the location of the discontinuities of rotation and CaII flux and log n(Li) for stars with masses lower than 102 Solar Masses, seems to reflect the sensitivity of these phenomena to the mass of the convective envelope. The drop in rotation, which results mostly from a magnetic braking, requires an increase in the mass of the convective envelope less than that required for the decrease in lithium abundance The location of the discontinuity in log n( Li) in the same region of the discontinuity ties in rotation and CaII flux, for stars with masses higher than 1.2 Solar Masses, may also be explained by the behavior of the deepening of the convective envelope. In contrast to the relationship between rotation and CaII flux the relationship between lithium abundance and rotation shows no dear tendency toward linear behavior. Similarly, the same tendency is observed in the relationship between lithium abundance and CaII flux in spite of these facts, subgiants with high lithium content also have high rotation and high CaII emission flux. We also observed that stars with high lithium content present, in its majority, an undeveloped convective envelope, whereas stars with low lithium content have a developed convective envelope. In the case of the rotation, stars with undeveloped convective envelope, show rotational velocities as much high as low, whereas stars with developed convective envelope only present low rotation / A conex?o entre rota??o, fluxo de emiss?o de CaII e abund?ncia de l?tio ? analisada para uma amostra de estrelas subgigantes, cujo status evolucion?rio foi determinado a partir do c?digo de Toulouse-Geneve e de medidas trigonom?tricas de paralaxe do HIPPARCOS. Observamos que a distribui??o da rota??o e do fluxo de emiss?o de CaII, como fun??o da temperatura efetiva, mostra uma descontinuidade localizada em torno do mesmo tipo espectral, F8IV. Estrelas localizadas no lado azul deste tipo espectral exibem uma elevada dispers?o nos valores de rota??o e de fluxo de CaII, enquanto que estrelas localizadas no lado vermelho de F8IV mostram essencialmente baixa rota??o e baixo fluxo de CaII. A intensidade deste decl?nio, entretanto, depende da massa estelar. A distribui??o das abund?ncias de l?tio tamb?m apresenta descontinuidade, por?m, com um comportamento um pouco mais complexo. Para subgigantes com massa menor ou em torno de 1.2 Massas Solares, esse decl?nio ? observado mais tarde do que aquele na rota??o e no fluxo de CaII, enquanto que para massas maiores do que 1.2 Massas Solares o decrescimento na abund?ncia do l?tio ? localizado ao redor do tipo espectral F8IV. A discrep?ncia entre a localiza??o das descontinuidades da rota??o e da emiss?o do fluxo de CaII e do log n(Li), para estrelas com massas menores do que 1.2 Massas Solares, parece refletir a sensibilidade dos fen?menos em rela??o ? massa da envolt?ria convectiva. A diminui??o abrupta na rota??o, que resulta principalmente de uma desacelera??o magn?tica, requer um aumento na massa da envolt?ria convectiva menor do que o requerido para o decrescimento nas abund?ncias de l?tio. A localiza??o da descontinuidade em log n(Li), na mesma regi?o das descontinuidades na rota??o e na emiss?o de fluxo de CaII para estrelas com massas maiores do que 1.2 Massas Solares, pode tamb?m ser explicada atrav?s do comportamento da profundidade da envolt?ria convectiva. Em contraste com a rela??o entre rota??o e fluxo de CaII, a rela??o entre abund?ncia de l?tio e rota??o mostra uma tend?ncia n?o muito clara para um comportamento linear. Similarmente, a mesma tend?ncia ? observada na rela??o entre abund?ncia de l?tio e fluxo de CaII. Apesar destes fatos, subgigantes com alto conte?do de l?tio tamb?m possuem alta rota??o e alta emiss?o de fluxo de CaII. Observamos tamb?m que estrelas com alto conte?do de l?tio apresentam, em sua maioria, uma envolt?ria convectiva pouco desenvolvida, enquanto que estrelas com baixo conte?do de l?tio possuem uma envolt?ria convectiva bastante desenvolvida. No caso da rota??o, estrelas com a envolt?ria convectiva pouco desenvolvida apresentam velocidades rotacionais tanto altas como baixas, enquanto que estrelas com a envolt?ria convectiva bem desenvolvida apresentam apenas baixa rota??o

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-22T19:34:38Z No. of bitstreams: 1 FranciscoRochaVasconcelosNeto_DISSERT.pdf: 36977983 bytes, checksum: 6906f10c6a683d1d25aa6f43a6f02c1d (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-07T21:50:12Z (GMT) No. of bitstreams: 1 FranciscoRochaVasconcelosNeto_DISSERT.pdf: 36977983 bytes, checksum: 6906f10c6a683d1d25aa6f43a6f02c1d (MD5) / Made available in DSpace on 2017-03-07T21:50:12Z (GMT). No. of bitstreams: 1 FranciscoRochaVasconcelosNeto_DISSERT.pdf: 36977983 bytes, checksum: 6906f10c6a683d1d25aa6f43a6f02c1d (MD5) Previous issue date: 2016-08-19 / A pesquisa de mestrado procura evidenciar e contribuir para a import?ncia da constru??o de espa?os, com ?nfase na urbanidade, a partir da cria??o de um projeto de arquitetura que integra e redesenha a malha urbana por meio da implanta??o de um edif?cio. O trabalho consiste no desenvolvimento do anteprojeto de arquitetura do Cais da Mem?ria, um complexo arquitet?nico para viv?ncias afetivas com o ambiente de Natal, que se dariam por meio da ocupa??o e deslocamento pelo territ?rio da cidade por terra, ?gua e ar, via um edif?cio de escala e espacialidade urbanas, com fun??es de centro de atividades culturais e esta??o intermodal do sistema de transporte p?blico de passageiros. Implantado ?s margens do rio Potengi, entre o centro antigo tombado, o monumento da Pedra do Ros?rio e a comunidade AEIS Passo da P?tria, o projeto objetivou a valoriza??o e integra??o dessas quatro ?reas entrincheiradas e de grande valor simb?lico e hist?rico para a cidade. O trabalho baseia-se e estabelece uma espinha dorsal a partir da estrutura curricular, dos conte?dos program?ticos das disciplinas cursadas no Programa de P?s-Gradua??o em Arquitetura, Projeto e Meio Ambiente da UFRN e bibliografia espec?fica. Dividido em seis cap?tulos, a pesquisa se inicia no referencial te?rico, extraindo as caracter?sticas espaciais para a proposta a partir do estudo de projetos antecedentes sobre o tema e, na sequ?ncia, estabelece a rela??o entre componentes e pr?-dimensionamento na programa??o arquitet?nica. Na seguinte etapa dos condicionamentos de projeto, determina o universo de estudo (terreno), analisa normas e legisla??es, realiza coleta de dados, submete os esbo?os a avalia??es clim?ticas e de ordem tect?nica e determina o conceito da proposta. Por fim, projeta com o lugar, elaborando e dissecando a proposta a partir repert?rio estudado. Dessa maneira, a pesquisa contribuiu para o aperfei?oamento de solu??es de arquitetura para complexos e edif?cios de atividades culturais, estabelecendo poss?veis caracter?sticas que devem possuir um espa?o dessa natureza para permitir uma urbanidade sadia por meio da acessibilidade, do encontro, da sociabilidade e da apropria??o de um lugar, usando-as como estrat?gia de revitaliza??o, renova??o e requalifica??o urbana por meio do desenho e ocupa??o de um edif?cio. / This thesis seeks to discuss urban quality through the city spatial development. The work is a preliminary study for the architectural project for the place called ?Cais da Mem?ria? (Memory Pier). This is an intermodal cultural complex for the affective experience of the city of Natal that happens by way of occupation and travel by land, water, and air. The building is designed at the urban scale and functions as a center for cultural activities and a station for public transport of diverse kinds. Located on the banks of the Potengi river, between the historic center, the monument called Pedra do Ros?rio, and the community known as AEIS Passo da P?tria, the project seeks to integrate and add value to these four areas of great symbolic and historic value to the city. The thesis is organized around the academic curriculum and the programmatic content of the classes taken during the Graduate Studies Program in Architecture, Design, and Environment of the Federal University of Rio Grande do Norte (UFRN) AND specific bibliography. The thesis is made up of five chapters. It begins with a preliminary study of the spatial characteristics, building on prior projects on the topic. Then establishes a relation between the components and a preliminary architectural design. Therefore, determines the universe of the study (the land), analyzes norms and laws, includes a collection of statistics, submits sketches and climatic and technical evaluations, and determines the concept of the proposal. Finally, the design of the place, elaborating and dissecting the proposal, given the repertoire studied. In this way, the research contributed to the improvement of architectural solutions for buildings with urban significance and cultural uses, with solutions that embrace and redesign the existing urban network and establish a possible profile that public space should have in order to allow the development of a healthier urban context through the meeting, sociability, and appropriation of the space.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-09T19:50:49Z No. of bitstreams: 1 TharcisyoSaESousaDuarte_TESE.pdf: 4000536 bytes, checksum: 76f14cacb9f32138dca1e02cb150ad3b (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-13T19:38:20Z (GMT) No. of bitstreams: 1 TharcisyoSaESousaDuarte_TESE.pdf: 4000536 bytes, checksum: 76f14cacb9f32138dca1e02cb150ad3b (MD5) / Made available in DSpace on 2017-03-13T19:38:21Z (GMT). No. of bitstreams: 1 TharcisyoSaESousaDuarte_TESE.pdf: 4000536 bytes, checksum: 76f14cacb9f32138dca1e02cb150ad3b (MD5) Previous issue date: 2016-05-20 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O estudo das estrelas do tipo-solar inclui naturalmente as estrelas an?logas e g?meas, que s?o estrelas id?nticas ao Sol. Estes objetos desempenham um papel fundamental no ?mbito da astrof?sica moderna, principalmente, na investiga??o da nossa estrela como um objeto comum. Dentre os diversos par?metros f?sicos observ?veis, a atividade magn?tica e cromosf?rica - para um conjunto de estrelas muito similares ao Sol (an?logas e g?meas) - s?o essenciais para compreendermos a evolu??o din?mica da atividade estelar em escalas de tempo da ordem de v?rios bilh?es de anos, isto ? tempo de vida de uma estrela do tipo-solar sobre a sequ?ncia principal. Neste trabalho, entre outros aspectos, investigaremos as rela??es existentes entre o per?odo de rota??o, abund?ncia de l?tio, atividade magn?tica e cromosf?rica, massa e idades destes grupos de estrelas. Analisaremos ainda as determina??es das idades de acordo com a t?cnica da girocronologia. O objetivo principal do nosso trabalho ? investigar a lei de decaimento de cada um dos destes par?metros com base em uma ampla amostra de estrelas classificadas como an?logas e g?meas solares. Nossos resultados deram origem a quatro publica??es em revistas indexadas, dos quais dois j? se encontram em modo ?impress?. Estes resultados mostram que as leis de evolu??o (decaimento da abund?ncia de l?tio, da rota??o e do campo magn?tico) s?o fortemente dependentes do tipo-estelar, mostrando-se mais nitidez para as estrelas an?logas e g?meas. / The interest in studying the objects similar to the Sun, stars labeled as solar-type stars, analogs and solar twins, brings in its essence an attempt to end out another reference star and, furthermore, provides an investigation of evolutionary dynamic of our star as a function of various parameters. For this, we used three distinct samples of observable data, 170 solar-type stars from BCool catalog and observed with spectropolarimeters ESPaDOnS e NARVAL, 88 solar-twin stars of HARPS surveys, and 20 solar-analog stars from Kepler. From these data, we have investigated mainly the correlation among the rotation period, lithium abundance and stellar age. For the BCool stars and solar-twin from HARPS, we have used the rotation period determined through of chromospheric activity, in the case of Kepler solar analogs, the rotation period it is derived from photometric modulation. The lithium abundance for most of the solar-type and solar-twin stars have been collected from literature, while for the solar analogs, the lithium abundance were determined in the LTE regime using Kurucz atmospheric models and the MOOG code. For stellar age, we have used the gyrochronology method, which was calibrated using the Sun and a selection of open clusters, to redetermine them and comparing them with those derived from standard isochronal. Our results indicate that exist a decay law for the rotation period as a function of lithium abundance. This correlation becomes more clear for the solar-analog and solartwin stars, even the rotation period being determined through distinct mechanisms for each case. For stellar ages, measured from standard isochronal and gyrochronology, we realized that they diverge considerably when the stars are older than the Sun. This result has also been investigated by van Saders et al. (2016) and reect our limitation about thestellar evolution and mixing mechanisms. Our work has resulted in five publications in indexed journals, two already in print format, one recently submitted and other in final stage of conclusion.