A rapidly melting ice sheet produces a distinctive geometry of sea level (SL) change. Thus, a network of SL observations may, in principle, be used to infer sources of meltwater flux. We outline a new method, based on a Kalman smoother, for using tide gauge observations to estimate the individual sources of global SL change. The Kalman smoother technique iteratively calculates the maximum likelihood estimate of Greenland and West Antarctic ice sheet melt rates at each time step, and it allows for data gaps while also permitting the estimation of non-linear trends. We have also implemented a fixed multi-model Kalman filter that allows us to rigorously account for additional contributions to SL changes, such as glacial isostatic adjustment and thermal expansion. We report on a series of detection experiments based on synthetic SL data that explore the feasibility of extracting source information from SL records before applying the new methodology to historical tide gauge records. In the historical tide gauge study we infer a global mean SL rise of ~1.5 ± 0.5 mm/yr up to 1970, followed by an acceleration to a rate of ~2.0 ± 0.5 mm/yr in 2008. In addition to its connection to SL, Greenland and its large ice sheet act as a barrier to storm systems traversing the North Atlantic. As a result of the interaction with Greenland, low-pressure systems located in the Irminger Sea, between Iceland and Greenland, often produce strong low-level winds. Through a combination of modeling and the analysis of rare in-situ observations, we explore the evolution of a lee cyclone that resulted in three high-speed-wind events in November 2004. Understanding Greenland’s role in these events is critical in our understanding of local weather in this region.

Self-assembling peptides have emerged as new nanobiomaterials and received considerable attention in the areas of nanoscience and biomedical engineering. In this category are ionic-complementary peptides, which contain a repeating charge distribution and alternating hydrophobic and hydrophilic residues in the amino acid sequence, leading to the unusual combination of amphiphilicity and ionic complementarity. Although their self-assembled nanostructures have been successfully applied as scaffoldings for tissue engineering, novel materials for regenerative medicine and nanocarriers for drug and gene/siRNA delivery, aspects of the assembly process remain unclear. Since many of these applications involve peptide-modified interfaces and surfaces, a better understanding and control of the peptide assembly on a surface are very crucial for future development of peptide-based applications in nano-biotechnology. This thesis contains two major parts: (i) fundamental study of the assembly of a model ionic-complementary peptide EAK16-II on surfaces and (ii) potential applications of such a peptide in surface modification and nanofabrication. In the fundamental study, EAK16-II assembly on negatively charged mica was first investigated via in-situ Atomic Force Microscopy (AFM). It was found that EAK16-II nanofiber growth on mica is surface-assisted and follows a nucleation and growth mechanism involving two steps: (i) adsorption of nanofibers and fiber clusters (from the bulk solution) on the surface to serve as the seeds and (ii) fiber elongation from the active ends of the seeds. Such a process can be controlled by adjusting the solution pH since it modulates the adsorption of the seeds and the growth rates. Unlike what is observed on mica, EAK16-II formed well-ordered nanofiber patterns with preferential orientations at angles of 60° or 120° to each other on hydrophobic highly ordered pyrolytic graphite (HOPG) surfaces, resembling the crystallographic structure of the graphite. Nanofiber formation on HOPG is also surface-assisted and adopts a nucleation and growth mechanism that can be affected by solution pH. The pH-dependent adsorption of peptides to HOPG is attributed to the resulting changes in peptide hydrophobicity. It was also found that EAK16-II assembly can be induced by the mechanical force of a tapping AFM tip. It occurs when the tip cuts the adsorbed EAK16-II nanofibers into segments that then serve as seeds for new nanofiber growth. This finding allows one to locally grow nanofibers at specific regions of the surface. The tip cutting has been combined with the effect that solution pH has on peptide assembly to develop a new AFM lithography method to fabricate local patterned peptide nanostructures on HOPG. To study the use of EAK16-II for surface modification applications, the wettability and stability of the peptide-modified surfaces were characterized. EAK16-II-modified mica becomes slightly hydrophobic as the water contact angle increases from <10° to 20.3 ± 2.9°. However, the hydrophobicity of the HOPG surface is significantly reduced, as reflected in a contact angle change from 71.2 ± 11.1° to 39.4 ± 4.3°. The EAK16-II-modified mica surface is stable in acidic solution, while the modified HOPG surface is stable in both acidic and alkaline solutions. The peptide-modified HOPG shows potential as a biocompatible electrode for (bio)molecular sensing. The ability of EAK16-II to form nanofibers on surfaces has also promoted research on peptide-based metallic nanowire fabrication. Our approach is to provide EAK16-II with metal ion binding ability by adding a GGH motif to the C-terminus. This new peptide EAK16(II)GGH has been found to form one-dimensional nanofibers while binding to Cu2+ ions. The dimensions of the nanofibers were significantly affected by the nature of the anions (SO42-, Cl- and NO3-) in the copper salt solution. This work demonstrates the potential usage of EAK16-II for nanowire fabrication.

Atlas Copco Surface Drilling Equipment is one of the leading manufacturers of surface drill rigs. To stay in the top segment it is of great importance to have a well functioning development strategy as well as rig functions that makes the work as easy as possible for the operator. In this master thesis one development strategy has been evaluated and a dub tip control has been developed from idea to test on rig.   Today the conventional method to position the drill is to use two joysticks with three axes each where each axis corresponds to one hydraulic actuator on the boom and feeder structure. The dub tip control system enables the operator to position the drill in Cartesian coordinates with only one 3-axes joystick. After the definition of the desired drill angle is done, the control system makes sure that this angle is obtained throughout the positioning motion. This system makes it considerably easier for an inexperienced operator to position the drill.   For development, simulation and verification of the control algorithms and regulators Matlab/Simulink has been used. To test the control system on rig, a configuration with LabVIEW together with a compactDAQ has been evaluated. LabVIEW is chosen because it provides the opportunity to create a user friendly graphical user interface. To use this configuration is however not recommended for persons with little or none experience from using LabVIEW.   This development strategy can be used for tests and verifications of control algorithms, but since neither Windows nor the compactDAQ are real time systems, there are solutions that are better but of course to a higher price.   The master thesis work has shown that it is possible to implement a dub tip control on a rig of this dimension. It has also concluded that compensated valves are necessary to achieve optimal performance of a velocity controlled dub tip positioning.

Self-assembling peptides have emerged as new nanobiomaterials and received considerable attention in the areas of nanoscience and biomedical engineering. In this category are ionic-complementary peptides, which contain a repeating charge distribution and alternating hydrophobic and hydrophilic residues in the amino acid sequence, leading to the unusual combination of amphiphilicity and ionic complementarity. Although their self-assembled nanostructures have been successfully applied as scaffoldings for tissue engineering, novel materials for regenerative medicine and nanocarriers for drug and gene/siRNA delivery, aspects of the assembly process remain unclear. Since many of these applications involve peptide-modified interfaces and surfaces, a better understanding and control of the peptide assembly on a surface are very crucial for future development of peptide-based applications in nano-biotechnology. This thesis contains two major parts: (i) fundamental study of the assembly of a model ionic-complementary peptide EAK16-II on surfaces and (ii) potential applications of such a peptide in surface modification and nanofabrication. In the fundamental study, EAK16-II assembly on negatively charged mica was first investigated via in-situ Atomic Force Microscopy (AFM). It was found that EAK16-II nanofiber growth on mica is surface-assisted and follows a nucleation and growth mechanism involving two steps: (i) adsorption of nanofibers and fiber clusters (from the bulk solution) on the surface to serve as the seeds and (ii) fiber elongation from the active ends of the seeds. Such a process can be controlled by adjusting the solution pH since it modulates the adsorption of the seeds and the growth rates. Unlike what is observed on mica, EAK16-II formed well-ordered nanofiber patterns with preferential orientations at angles of 60° or 120° to each other on hydrophobic highly ordered pyrolytic graphite (HOPG) surfaces, resembling the crystallographic structure of the graphite. Nanofiber formation on HOPG is also surface-assisted and adopts a nucleation and growth mechanism that can be affected by solution pH. The pH-dependent adsorption of peptides to HOPG is attributed to the resulting changes in peptide hydrophobicity. It was also found that EAK16-II assembly can be induced by the mechanical force of a tapping AFM tip. It occurs when the tip cuts the adsorbed EAK16-II nanofibers into segments that then serve as seeds for new nanofiber growth. This finding allows one to locally grow nanofibers at specific regions of the surface. The tip cutting has been combined with the effect that solution pH has on peptide assembly to develop a new AFM lithography method to fabricate local patterned peptide nanostructures on HOPG. To study the use of EAK16-II for surface modification applications, the wettability and stability of the peptide-modified surfaces were characterized. EAK16-II-modified mica becomes slightly hydrophobic as the water contact angle increases from <10° to 20.3 ± 2.9°. However, the hydrophobicity of the HOPG surface is significantly reduced, as reflected in a contact angle change from 71.2 ± 11.1° to 39.4 ± 4.3°. The EAK16-II-modified mica surface is stable in acidic solution, while the modified HOPG surface is stable in both acidic and alkaline solutions. The peptide-modified HOPG shows potential as a biocompatible electrode for (bio)molecular sensing. The ability of EAK16-II to form nanofibers on surfaces has also promoted research on peptide-based metallic nanowire fabrication. Our approach is to provide EAK16-II with metal ion binding ability by adding a GGH motif to the C-terminus. This new peptide EAK16(II)GGH has been found to form one-dimensional nanofibers while binding to Cu2+ ions. The dimensions of the nanofibers were significantly affected by the nature of the anions (SO42-, Cl- and NO3-) in the copper salt solution. This work demonstrates the potential usage of EAK16-II for nanowire fabrication.

A method to find solutions to multi-objective design problems that involve poor information available was proposed. The method quantified the designers intuition in a systematic manner, and utilized it to approximate inaccurate and/or vague numbers. In the context of possibility theory, uncertain values were expressed through possibility distributions, i.e. fuzzy membership functions. Based on the membership functions of the value, levels of confidence of the solutions to multi-objective problems were defined through the notions of possibility and necessity. An evolutionary algorithm was modified to find sets of solutions that allow certain levels of confidence instead of the crisp sets of the solutions. The method was applied to a design problem of the gyrodyne configuration and sets of the solutions of the specified possibility and necessity were found. The results of the design problem and the suggestions for future research were discussed.

Film cooling effectiveness on a gas turbine blade tip on the near tip pressure side and on the squealer cavity floor is investigated. Optimal arrangement of film cooling holes, effect of a full squealer and a cutback squealer, varying blowing ratios and squealer cavity depth are also examined on film cooling effectiveness. The film-cooling effectiveness distributions are measured on the blade tip, near tip pressure side and the inner pressure and suction side rim walls using a Pressure Sensitive Paint (PSP) technique. A blowing ratio of 1.0 is found to give best results on the pressure side whereas the other tip surfaces give best results for blowing ratios of 2. Film cooling effectiveness tests are also performed on the span of a fully-cooled high pressure turbine blade in a 5 bladed linear cascade using the PSP technique. Film cooling effectiveness over the entire blade region is determined from full coverage film cooling, showerhead cooling and from each individual row with and without an upstream wake. The effect of superposition of film cooling effectiveness from each individual row is then compared with full coverage film cooling. Results show that an upstream wake can result in lower film cooling effectiveness on the blade. Effectiveness magnitudes from superposition of effectiveness data from individual rows are comparable with that from full coverage film cooling. Internal heat transfer measurements are also performed in a high aspect ratio channel and from jet array impingement on a turbulated target wall at large Reynolds numbers. For the channel, three dimple and one discrete rib configurations are tested on one of the wide walls for Reynolds numbers up to 1.3 million. The presence of a turbulated wall and its effect on heat transfer enhancement against a smooth surface is investigated. Heat transfer enhancement is found to decrease at high Re with the discrete rib configurations providing the best enhancement but highest pressure losses. Experiments to investigate heat transfer and pressure loss from jet array impingement are also performed on the target wall at Reynolds numbers up to 450,000. The heat transfer from a turbulated target wall and two jet plates is investigated. A target wall with short pins provides the best heat transfer with the dimpled target wall giving the lowest heat transfer among the three geometries studied.

This study was conducted to determine the survival of Escherichia coli O157:H7 and Salmonella Typhimurium in marinade that was used to vacuum tumble beef inside skirts and beef tri-tip roasts. The depth of penetration of each microorganism into the individual meat products, and the survival of these microorganisms in the products as well as marinade stored over time were evaluated. Two commercial marinades were used, Reo TAMU Fajita Marinade and Legg's Cajun Style Marinade. Eighteen beef inside skirts and 18 tri-tips were used during this study. Both inside skirts and tri-tips were vacuum tumbled for a total of 1 h. Samples of products were tested immediately following tumbling (day 0), or were vacuum packaged and stored in the cooler (approximately 2 degrees C) to be tested 7 and 14 days following tumbling. Samples of the spent marinade were taken and tested initially following tumbling (day 0), and were also stored in a cooler and tested 3 and 7 days after the marinade was used. The results of the study showed that with both marinades S. Typhimurium and E. coli O157:H7 penetrated throughout the skirt meat. After having been stored for 7 days following tumbling, the log value of both S. Typhimurium and E. coli O157:H7 decreased in the meat. After 14 days of storage following tumbling, the log value of both S. Typhimurium and E. coli O157:H7 continued to decrease; however, both pathogens were still detectable. The penetration of the pathogens in the tri-tip roast varied depending on the thickness of the roast. The thicker roasts had undetectable levels of both pathogens in the geometric center; however, the thinner tri-tip roasts had detectable levels at the geometric center. The spent marinade tested on day 0, 3, and 7 showed that the microorganisms were able to survive in the marinade at refrigerated temperatures. The results of this study demonstrated that pathogens may penetrate into the interior of beef skirts and tri-tips during vacuum tumbling with contaminated marinade, and that pathogens survive during refrigerated storage of spent marinade. Industry should consider these data when evaluating potential food safety risks associated with the production of vacuum tumbling beef products.

The inlet temperature of modern gas turbine engines has been increased to achieve higher thermal efficiency and increased output. The blade edge regions, including the blade tip, the leading edge, and the platform, are exposed to the most extreme heat loads, and therefore, must be adequately cooled to maintain safety. For the blade tip, there is tip leakage flow due to the pressure gradient across the tip. This leakage flow not only reduces the blade aerodynamic performance, but also yields a high heat load due to the thin boundary layer and high speed. Various tip configurations, such as plane tip, double side squealer tip, and single suction side squealer tip, have been studied to find which one is the best configuration to reduce the tip leakage flow and the heat load. In addition to the flow and heat transfer on the blade tip, film cooling with various arrangements, including camber line, upstream, and two row configurations, have been studied. Besides these cases of low inlet/outlet pressure ratio, low temperature, non-rotating, the high inlet/outlet pressure ratio, high temperature, and rotating cases have been investigated, since they are closer to real turbine working conditions. The leading edge of the rotor blade experiences high heat transfer because of the stagnation flow. Film cooling on the rotor leading edge in a 1-1/2 turbine stage has been numerically studied for the design and off-design conditions. Simulations find that the increasing rotating speed shifts the stagnation line from the pressure side, to the leading edge and the suction side, while film cooling protection moves in the reverse direction with decreasing cooling effectiveness. Film cooling brings a high unsteady intensity of the heat transfer coefficient, especially on the suction side. The unsteady intensity of film cooling effectiveness is higher than that of the heat transfer coefficient. The film cooling on the rotor platform has gained significant attention due to the usage of low-aspect ratio and low-solidity turbine designs. Film cooling and its heat transfer are strongly influenced by the secondary flow of the end-wall and the stator-rotor interaction. Numerical predictions have been performed for the film cooling on the rotating platform of a whole turbine stage. The design conditions yield a high cooling effectiveness and decrease the cooling effectiveness unsteady intensity, while the high rpm condition dramatically reduces the film cooling effectiveness. High purge flow rates provide a better cooling protection. In addition, the impact of the turbine work process on film cooling effectiveness and heat transfer coefficient has been investigated. The overall cooling effectiveness shows a higher value than the adiabatic effectiveness does.

Detailed film cooling effectiveness distributions were measured on the stationary blade tip and on the leading edge region of a rotating blade using a Pressure Sensitive Paint technique. Air and nitrogen gas were used as the film cooling gases and the oxygen concentration distribution for each case was measured. The film cooling effectiveness information was obtained from the difference of the oxygen concentration between air and nitrogen gas cases by applying the mass transfer analogy. In the case of the stationary blade tip, plane tip and squealer tip blades were used while the film cooling holes were located (a) along the camber line on the tip or (b) along the span of the pressure side. The average blowing ratio of the cooling gas was controlled to be 0.5, 1.0, and 2.0. Tests were conducted in a five-bladed linear cascade with a blow down facility. The free stream Reynolds number, based on the axial chord length and the exit velocity, was 1,100,000 and the inlet and the exit Mach number were 0.25 and 0.59, respectively. Turbulence intensity level at the cascade inlet was 9.7%. All measurements were made at three different tip gap clearances of 1%, 1.5%, and 2.5% of blade span. Results show that the locations of the film cooling holes and the presence of squealer have significant effects on surface static pressure and film-cooling effectiveness. Same technique was applied to the rotating turbine blade leading edge region. Tests were conducted on the first stage rotor of a 3-stage axial turbine. The Reynolds number based on the axial chord length and the exit velocity was 200,000 and the total to exit pressure ratio was 1.12 for the first rotor. The effects of the rotational speed and the blowing ratio were studied. The rotational speed was controlled to be 2400, 2550, and 3000 rpm and the blowing ratio was 0.5, 1.0, and 2.0. Two different film cooling hole geometries were used; 2-row and 3-row film cooling holes. Results show that the rotational speed changes the directions of the coolant flows. Blowing ratio also changes the distributions of the coolant flows. The results of this study will be helpful in understanding the physical phenomena regarding the film injection and designing more efficient turbine blades.

This thesis investigated a tip-over stability of mobile boom cranes with swinging payloads. Base and crane motion presents a tip-over problem. Attaching complex payloads further complicates the problem. They study began with a single-pendulum payload to analyze a tip-over stability characteristics under different conditions. A simple tip-over prediction model was developed with a goal of limiting a computational cost to a minimum. The stability was characterized by a tip-over stability margin method. The crane's tip-over stability was also represented by the maximum possible payload it can carry throughout the workspace. In a static stability analysis, mobile boom crane was assumed to be stationary, thus with no payload swing. The study provided basic understanding on the relationship between tip-over stability and boom configuration. In a pseudo-dynamic stability analysis, the method incorporated payload swing into the analysis by adding estimated maximum payload swing due to motions. To estimate the angles, differential equations of motions of payload swings were derived. The thesis extended the study to a double-pendulum payload. The maximum swing angles estimated in the single-pendulum case were directly applied to the double-pendulum case. To validate the analytical methods, a full dynamic multi-body simulation model of a mobile boom crane was developed. The predictions from the previous analysis were verified by the simulation results. The prediction model and the analytical methods in the thesis provide a significant tool for practical application of tip-over stability analysis on mobile boom cranes. The experimental results increase the confidence of the study's accuracy.