Les synapses fibres moussues de l‘hippocampe entre le gyrus denté et les cellulespyramidales de CA3 sont caractérisées par leur morphologie particulière, et par leurspropriétés distinctives de transmission synaptique et de plasticité présynaptique. Cessynapses sont parfois appelées «détonatrices» pour leur rôle fonctionnel dansl‘encodage de la mémoire épisodique. Cependant, les mécanismes moléculaires à labase des propriétés spécifiques de ces synapses restent peu connus. Ce travail estcomposé de deux parties principales:1) Phénotypage des synapses fibres moussues de l'hippocampe chez les sourisVAMP7 KOVAMP7 est une protéine SNARE vésiculaire de la famille des longins, qui joue unrôle dans la croissance des neurites durant le développement. Dans le cerveauadulte, VAMP7 est enrichi dans un sous-ensemble de terminaisons nerveuses, enparticulier dans les fibres moussues de l‗hippocampe. Nous avons analysé lafonction de VAMP7 dans la libération de neurotransmetteurs par une caractérisationextensive de la transmission synaptique et des mécanismes de plasticité de cettesynapse. L'absence de VAMP7 ne cause pas de graves déficits développementauxou neuronaux (Sato et al., 2011; Danglot et al., 2012). Les mécanismesprésynaptiques de la plasticité à court terme de la fibre moussue de l‘hippocampesemblent également normaux, pour des raisons éventuelles qui seront discutées.2) Circuits du CA3 examinés par traçage viral et enregistrements de pairesNous avons développé une technique pour établir des enregistrements en pairesentre cellules en grain du gyrus denté connectées et cellules pyramidales CA3 (GCCA3),sur des cultures organotypiques de tranches d'hippocampe de souris. Pouridentifier les partenaires présynaptiques directs à une cellule pyramidale CA3 ciblée,nous avons combiné l‘électroporation cellulaire unitaire et le traçage mono-transsynaptiquebasé sur un virus de la rage recombinant et pseudotypé. Nous avonstransfecté une cellule pyramidale CA3 unique par tranche avec les plasmides codantla glycoprotéine d‘enveloppe du virus de la rage (RG), un rapporteur fluorescent, etla protéine TVA (récepteur de surface apparenté au EnvA, qui n'a pas d‘homologuechez les cellules de mammifères). Les tranches ont ensuite été infectées avec levirus de la rage recombinant et pseudotypé. Après 3-4 jours, le traçage mono-transsynaptiquerévèle les entrées présynaptiques de ce neurone unique. Ensuite, nousavons pu établir des enregistrements de paires entre les cellules en grain-CA3connectés, ainsi que de quantifier les partenaires présynaptiques de la cellulepyramidale CA3 de départ. / The hippocampal mossy fiber is characterized by its particular morphology, distinctsynaptic transmission and presynaptic plasticity. Moreover, this synapse has beencalled ―teacher‖ or ―detonator‖ for its proposed functional role in episodic memoryencoding. Nevertheless, the molecular mechanisms underlying its specific functionalproperties remain elusive. This work is composed of two main parts:1) Phenotyping Hippocampal Mossy Fiber Synapses in VAMP7 KO MiceVAMP7 is a vesicle SNARE of the longin family important in neurite growth duringdevelopment. In the adult brain, VAMP7 is enriched in a subset of nerve terminals,particularly at the hippocampal mossy fiber. We analyzed VAMP7 function inneurotransmitter release by characterizing basal and evoked transmission at thissynapse in KO mice and fully tested hypotheses relevant to short-term plasticity.Loss of VAMP7 has been previously reported not to cause major developmental orneurological deficits (Sato et al., 2011; Danglot et al., 2012). Presynapticmechanisms of short-term plasticity at the hippocampal mossy fiber also seemunaffected for potential reasons that will be discussed.2) CA3 Circuits Probed with RABV-Tracing and Paired RecordingsWe developed a technique to establish paired recordings between connected dentategyrus granule cells and CA3 pyramidal cells (GC-CA3) in mouse hippocampalorganotypic slice cultures. To identify direct presynaptic partners to a defined targetCA3 pyramidal cell, we combined single-cell electroporation (SCE) and mono-transsynaptictracing based on a pseudotyped, recombinant rabies virus (EnvApseudotyped RABV ΔG). Using SCE we transfected a single CA3 pyramidal cell perslice with the plasmids encoding: the RABV envelope glycoprotein (RG), afluorescent reporter, and TVA (the EnvA cognate surface receptor, which has nohomologue in mammalian cells). The slices were subsequently infected with EnvApseudotyped RABV ΔG. After 3-4 days, the RABV mono-trans-synaptic tracingrevealed the presynaptic inputs of that single neuron. Then, we were able toestablish paired recordings between connected GC-CA3 cells, as well as to quantifythe presynaptic partners of the starter CA3 pyramidal cell. / De mosvezel van de hippocampus kenmerkt zich door een bijzondere morfologie,uitzonderlijke synaptische transmissie en presynaptische plasticiteit. De synapswordt ook wel "leraar" of "detonator" genoemd vanwege zijn waarschijnlijke rol in decodering van het episodisch geheugen. Toch blijven de specifieke moleculairemechanismen van dit synaps onbekend. Dit werk bestaat uit twee delen:1) Fenotypering van mosvezel synapsen van de hippocampus in VAMP7 KO muizenVAMP7 is een vesicle-SNARE van de longin familie van belang bij de groei vanneurieten tijdens de ontwikkeling. In de volwassen hersenen, wordt VAMP7 verrijkt ineen subset van zenuwuiteinden, vooral in de mosvezel van de hippocampus. Weanalyseerden VAMP7 functie in neurotransmitter afgifte door het karakteriseren vanbasale en opgeroepen transmissie bij deze synaps in KO muizen. Eerder is algesteld dat gebrek aan VAMP7 niet leidt tot grote ontwikkelings- of neurologischeafwijkingen (Sato et al., 2011; Danglot et al., 2012). Presynaptische mechanismenvan korte termijn plasticiteit in de mosvezel van de hippocampus lijken ookonaangetast te zijn, de mogelijke redenen hiervoor zullen worden besproken.2) CA3 circuits onderzocht met behulp van RABV-tracing en gekoppelde opnamesWe ontwikkelden een techniek om gekoppelde opnames tussen korelcellen van degyrus dentatus en aangesloten CA3 piramidale cellen (KC-CA3) op zogenaamde‗mouse hippocampal organotypic slice cultures‘ te meten. Om rechtstreeksepresynaptische partners te identificeren van een specifieke CA3 piramidale cel,combineerden we single-cell electroporation (SCE) en mono-trans-synaptic tracingop basis van een pseudo-typed, recombinant rabiësvirus (EnvA pseudogetypedRABV ΔG). Met behulp van SCE transfecteerde we één CA3 piramidale cel per slicemet plasmiden die coderen voor: het RABV glycoproteïne-envelop (RG), eenfluorescerende reporter, en TVA (de aan EnvA verwante oppervlakte receptor diegeen homoloog in zoogdiercellen heeft). De slices werden vervolgens geïnfecteerdmet ENVA pseudogetyped RABV ΔG. Na 3-4 dagen bracht de RABV mono-transsynaptischetracing de presynaptische ingangen van die ene neuron aan het licht.Hierna konden we gekoppelde opnames doen tussen verbonden KC-CA3 cellen.Daarnaast konden we de presynaptische partners van de starter CA3 pyramidale celkwantificeren.

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Building physics as a branch of architecture must ensure an indoor comfort of each user and inhabitant of a building object. This involves, acoustics, indoor thermal conditions and among others also daylighting and artificial lighting of buildings. Light as a particle and an electromagnetic wave, is required by the different aspects of the human organism. It allows the living beings to see, influences skin and bones, the biorhythms, etc. Therefore, it is necessary for the engineering community to predict the correct illuminance and luminance levels acting insides. The thesis deals with such issues. More precisely, it assesses the quality of design tools and methodologies, either against CIE reference cases described in CIE 171/2006 and against real measurements done over the working plane of an indoor space located in the attic of Building D of the Institute of Building Structures, Faculty of Civil Engineering, Brno University of Technology, too. The tools tested throughout the solution of the dissertation did involve three computer programs: RADIANCE, WDLS v3.1 and WDLS v4.1, and one numerographical approach, namely the Daniljuk’s innovated methodology (sometimes even combined with the theories of BRS). In addition several software’s have had been created alongside the process assessment, just to mention the “RADIANCE Script”, “RADIANCE Data Evaluation Script” or “MuuLUX“. The later was written as a communication software allowing the connection of the KONICA-MINOLTA T10 illuminance meter to a computer with the aim of data collection while long term observation. The solution did also require the establishment of a measuring element for the determination of the light reflectance values of surfaces. The solutions, results and conclusions do describe how well did the design approaches deal while predicting the resulting awaited daylight factor levels in points over the working plane.

完善的崩塌地資料庫有助於地區土地利用的適宜性評估、與環境保護措施之研訂。目前，崩塌地地理空間資料(Geospatial data)的產生方法主要為：人為判釋經正射糾正(Ortho-rectification)的遙測影像，基於該影像，將辨識目標數位化(Digitizing)。然而，遙測影像的「正射糾正」與「人為判釋」往往不敷災後的緊急需求。為促進資料收集效率，本研究試圖發展一套自動化流程：以「未經糾正的遙測影像」為判釋對象，判釋作業以「物件式影像分類(Object-based classification)技術」進行，並利用「現存地形資料」，實現自動判釋所產生之辨識成果的地理對位(Georeferencing)與過濾篩選；最後，以「與現存各類輔助資料的套疊分析成果」為其屬性，以便利崩塌地地理空間資料的後續應用。 物件式影像分類分為為「影像分割(Image segmentation)」與「物件分類」兩步驟。於影像分割階段，採用多重解析度分割法(Multiresolution segmentation algorithm)─由於陰影下各類地物的影像光譜差異較不明顯，為避免陰影區之錯誤分割，賦予陰影區較小的尺度參數(Scale parameter)；於物件分類階段，基於訓練資料，以「線性核函數的支持向量機(Support Vector Machine, SVM, with a linear kernel)」為分類器，偵測「非雲與植被區」，並輸出為向量式資料(Vector data)。而後基於現存地形資料，以光線追蹤法(Ray-tracing algorithm)進行分類器輸出向量式資料的地理對位，並自訂第二階段的地形特徵過濾準則。實驗成果顯示，此自動化流程產出的崩塌地地理空間資料─其生產者精度(Producer’s accuracy)與使用者精度(User’s accuracy)分別介於0.85~0.99與0.44~0.96。

Simuler efficacement l'éclairage global est l'un des problèmes ouverts les plus importants en infographie. Calculer avec précision les effets de l'éclairage indirect, causés par des rebonds secondaires de la lumière sur des surfaces d'une scène 3D, est généralement un processus coûteux et souvent résolu en utilisant des algorithmes tels que le path tracing ou photon mapping. Ces techniquesrésolvent numériquement l'équation du rendu en utilisant un lancer de rayons Monte Carlo. Ward et al. ont proposé une technique nommée irradiance caching afin d'accélérer les techniques précédentes lors du calcul de la composante indirecte de l'éclairage global sur les surfaces diffuses. Krivanek a étendu l'approche de Ward et Heckbert pour traiter le cas plus complexe des surfaces spéculaires, en introduisant une approche nommée radiance caching. Jarosz et al. et Schwarzhaupt et al. ont proposé un modèle utilisant le hessien et l'information de visibilité pour raffiner le positionnement des points de la cache dans la scène, raffiner de manière significative la qualité et la performance des approches précédentes. Dans ce mémoire, nous avons étendu les approches introduites dans les travaux précédents au problème du radiance caching pour améliorer le positionnement des éléments de la cache. Nous avons aussi découvert un problème important négligé dans les travaux précédents en raison du choix des scènes de test. Nous avons fait une étude préliminaire sur ce problème et nous avons trouvé deux solutions potentielles qui méritent une recherche plus approfondie. / Efficiently simulating global illumination is one of the most important open problems in computer graphics. Accurately computing the effects of indirect illumination, caused by secondary bounces of light off surfaces in a 3D scene, is generally an expensive process and often solved using algorithms such as path tracing or photon mapping. These approaches numerically solve the rendering equation using stochastic Monte Carlo ray tracing. Ward et al. proposed irradiance caching to accelerate these techniques when computing the indirect illumination component on diffuse surfaces. Krivanek extended the approach of Ward and Heckbert to handle the more complex case of glossy surfaces, introducing an approach referred to as radiance caching. Jarosz et al. and Schwarzhaupt et al. proposed a more accurate visibility-aware Hessian-based model to greatly improve the placement of records in the scene for use in an irradiance caching context, significantly increasing the quality and performance of the baseline approach. In this thesis, we extended similar approaches introduced in these aforementioned work to the problem of radiance caching to improve the placement of records. We also discovered a crucial problem overlooked in the previous work due to the choice of test scenes. We did a preliminary study of this problem, and found several potential solutions worth further investigation.

Propriété, droit réel sur la chose d’autrui, opposabilité, droit de suite, droit de préférence, exclusivité, meubles, immeubles, accession, accessoire, subrogation réelle, fongibilité, universalité, etc. la liste est longue des concepts forgés par le droit des biens que sollicitent les sûretés réelles, non parfois sans les malmener, afin d’atteindre leur finalité propre qu’est la garantie du paiement des créances de somme d’argent. Après plusieurs thèses, articles et monographies consacrés à tel ou tel aspect de ces interactions, la présente thèse livre une étude globale des rapports noués entre les deux disciplines afin de mettre en évidence leurs points de rencontre, leurs points de divergence et, le cas échéant, de possibles enrichissements réciproques. / Property, real rights, tracing, exclusive possession, movables, immovables, accession, accessory obligation, real subrogation, fungibles, funds, etc. are a few of the many concepts forged by property law that are used and sometimes misused, in the law relating to security transactions. The misuse of such concepts in the latter area of law can be attributed to the different purpose that underlies such transactions, being specifically the guaranteeing of debt repayment. After several theses, essays and monographs have been devoted to particular aspects of these interactions between property law and the law surrounding security transactions, this thesis delivers a comprehensive study about the relationship established between the two areas of law, and will highlight their points of convergence, points of divergence and mutual efficiency.

Goal of this work is to write an optimized program for visualization of 3D scenes using ray tracing method. First, the theory of ray tracing together with particular techniques are presented. Next part focuses on different approaches to accelerate the algorithm. These are space partitioning structures, fast ray-triangle intersection technique and possibilities to parallelize the whole ray tracing method. A standalone chapter addresses the design and implementation of the ray tracing program.

>Magister Scientiae - MSc / An increasing number of measurements reveal the presence of a low-energy enhancement in the gamma-ray strength function (GSF). The GSF, which is the ability of nuclei to absorb or emit rays, provides insight into the statistical properties of atomic nuclei. For this project the GSF was studied for 74Ge which was populated in the reaction 74Ge(p,p')74Ge* at a beam energy of 18 MeV. The data were collected with the STARS-LIBERACE array at Lawrence Berkeley National Laboratory. Silicon detector telescopes were used for particle identi cation and rays in coincidence were detected with 5 clover-type high-purity germanium detectors. Through the analysis particle- - coincidence events were constructed. These events, together with well-known energy levels, were used to identify primary rays from the quasicontinuum. Primary rays from a broad excitation energy region, which decay to six 2+ states could be identi ed. These states and the associated primary rays are used to measure the GSF for 74Ge with the Ratio Method [1], which entails taking ratios of e ciency-corrected primary -ray intensities from the quasicontinuum. Results from the analysis of the data and focus on the existence of the low-energy enhancement in 74Ge will be discussed. The results are further discussed in the context of other work done on 74Ge using the ( , ') [2], (3He,3He') [3] and ( , ') [4] reactions.

[pt] O problema de localização de estações móveis pessoais em sistemas celulares de comunicações vem recebendo grande atenção nos últimos anos, tanto por questões ligadas à segurança como por suas amplas aplicações comerciais no desenvolvimento de novos serviços e aplicações. Nesta dissertação foi desenvolvido um ambiente de simulação de localização de estações móveis em ambiente micro celulares empregando um programa de traçado de raios pelo método da força bruta (lançamento de raios), já disponível, para estimar os comprimentos de percursos e tempos de chegada entre diversas estações rádio base e a estação móvel em cenários urbanos modelados por sólidos multifacetados. Os perfis de retardo gerados por este programa são usados como dados de entrada para um programa desenvolvido nesta dissertação que estima a localização dos móveis utilizando os métodos de Taylor e de Chan. O processo desenvolvido foi testado em ambientes de geometria simples fornecendo resultados bastante consistentes e mostrando que a técnica de traçado de raios é uma ferramenta útil para a simulação e desenvolvimento de algoritmos de localização, cujo teste em situações reais exige grande volume de medidas de alta complexidade cujos exemplos na literatura técnica são escassos. Com base nas simulações foi investigadas a influência do número de estações rádio base na precisão das estimativas de localização e realizada uma comparação do desempenho dos métodos em situações com visibilidade (LOS) e sem visibilidade (NLOS). Foi analisado ainda o efeito da altura das estações na precisão dos resultados de localização. / [en] The location of mobile terminals in mobile cellular systems has been receiving increasing attention in the last few years. This interest in focused not only in security aspects but also in the development of new services for commercial application. In this Dissertation a simulation environment for mobile stations location in microcellular systems was developed. The simulation tools include a ray tracing software, previously implemented using the ray launching technique, to estimate the path lengths and time of arrival of signals from the mobile station to several radio base stations, and new software implementing terminal location methods using the Taylor linearization and the Chan methods. The simulation tools were tested in scenarios of simple geometry producing consistent results and showing that ray tracing can be a useful tool for simulation and development of location algorithms. The simulations allowed the investigation of location precision dependence on the number of radio bases employed and the evaluation of the estimation methods in visibility (LOS) and non- visibility (NLOS) conditions. The influence of base station antennas heights was also investigated.